The Raman fingerprint spectral region provides abundant structural information on molecules.However,analyzing vibrational images within this region using coherent Raman imaging remains challenging due to the small Ram...The Raman fingerprint spectral region provides abundant structural information on molecules.However,analyzing vibrational images within this region using coherent Raman imaging remains challenging due to the small Raman cross section and congested spectral features.In this study,we combined ultrabroadband coherent anti-Stokes Raman scattering(CARS)microspectroscopy across the spectral range of 500−4000 cm^(−1)with multivariate curve resolution-alternating least-squares(MCRALS)to reveal hidden Raman bands in the fingerprint region.Applying this method to mouse brain tissue,we extracted information on cholesterol and collagen,leveraging their distinctive molecular signatures,as well as on key molecules such as lipids,proteins,water,and nucleic acids.Moreover,the simultaneous detection of second harmonic generation facilitated labelfree visualization of organelles,including arachnoid membrane and Rootletin filaments.展开更多
基金supported by the Japan Society for the Promotion of Science(JSPS)through a Grant-in-Aid for Scientific Research(A)(KAKENHI,grant number 21H04961)Japan Science and the Technology Agency(JST)through a Mirai Program grant(JPMJMI22G5)+1 种基金It also benefited from a Japan−France bilateral project awarded to H.K.and the support of the French government in the form of a grant managed by the National Research Agency under the Investments for the Future program(ANR-10-LABX-0074 Sigma-LIM)awarded to P.Lsupported by AMED under Grant Number JP21zf0127005.
文摘The Raman fingerprint spectral region provides abundant structural information on molecules.However,analyzing vibrational images within this region using coherent Raman imaging remains challenging due to the small Raman cross section and congested spectral features.In this study,we combined ultrabroadband coherent anti-Stokes Raman scattering(CARS)microspectroscopy across the spectral range of 500−4000 cm^(−1)with multivariate curve resolution-alternating least-squares(MCRALS)to reveal hidden Raman bands in the fingerprint region.Applying this method to mouse brain tissue,we extracted information on cholesterol and collagen,leveraging their distinctive molecular signatures,as well as on key molecules such as lipids,proteins,water,and nucleic acids.Moreover,the simultaneous detection of second harmonic generation facilitated labelfree visualization of organelles,including arachnoid membrane and Rootletin filaments.
