Responding to the stochasticity and uncertainty in the power height of distributed photovoltaic power generation.This paper presents a distributed photovoltaic ultra-short-term power forecasting method based on Variat...Responding to the stochasticity and uncertainty in the power height of distributed photovoltaic power generation.This paper presents a distributed photovoltaic ultra-short-term power forecasting method based on Variational Mode Decomposition(VMD)and Channel Attention Mechanism.First,Pearson’s correlation coefficient was utilized to filter out the meteorological factors that had a high impact on historical power.Second,the distributed PV power data were decomposed into a relatively smooth power series with different fluctuation patterns using variational modal decomposition(VMD).Finally,the reconstructed distributed PV power as well as other features are input into the combined CNN-SENet-BiLSTM model.In this model,the convolutional neural network(CNN)and channel attention mechanism dynamically adjust the weights while capturing the spatial features of the input data to improve the discriminative ability of key features.The extracted data is then fed into the bidirectional long short-term memory network(BiLSTM)to capture the time-series features,and the final output is the prediction result.The verification is conducted using a dataset from a distributed photovoltaic power station in the Northwest region of China.The results show that compared with other prediction methods,the method proposed in this paper has a higher prediction accuracy,which helps to improve the proportion of distributed PV access to the grid,and can guarantee the safe and stable operation of the power grid.展开更多
Traditional research believes that the filling body can effectively control stress concentration while ignoring the problems of unknown stability and the complex and changeable stress distribution of the filling body...Traditional research believes that the filling body can effectively control stress concentration while ignoring the problems of unknown stability and the complex and changeable stress distribution of the filling body–surrounding rock combination under high-stress conditions.Current monitoring data processing methods cannot fully consider the complexity of monitoring objects,the diversity of monitoring methods,and the dynamics of monitoring data.To solve this problem,this paper proposes a phase space reconstruction and stability prediction method to process heterogeneous information of backfill–surrounding rock combinations.The three-dimensional monitoring system of a large-area filling body–surrounding rock combination in Longshou Mine was constructed by using drilling stress,multipoint displacement meter,and inclinometer.Varied information,such as the stress and displacement of the filling body–surrounding rock combination,was continuously obtained.Combined with the average mutual information method and the false nearest neighbor point method,the phase space of the heterogeneous information of the filling body–surrounding rock combination was then constructed.In this paper,the distance between the phase point and its nearest point was used as the index evaluation distance to evaluate the stability of the filling body–surrounding rock combination.The evaluated distances(ED)revealed a high sensitivity to the stability of the filling body–surrounding rock combination.The new method was then applied to calculate the time series of historically ED for 12 measuring points located at Longshou Mine.The moments of mutation in these time series were at least 3 months ahead of the roadway return dates.In the ED prediction experiments,the autoregressive integrated moving average model showed a higher prediction accuracy than the deep learning models(long short-term memory and Transformer).Furthermore,the root-mean-square error distribution of the prediction results peaked at 0.26,thus outperforming the no-prediction method in 70%of the cases.展开更多
An effective in vitro propagation protocol was designed for Lavandula angustifolia Miller,a medicinal aromatic plant that is a prominent source of volatile organic compounds(VOCs).Murashige and Skoog media were supple...An effective in vitro propagation protocol was designed for Lavandula angustifolia Miller,a medicinal aromatic plant that is a prominent source of volatile organic compounds(VOCs).Murashige and Skoog media were supplemented with various concentrations of Plant Growth Regulators(PGRs),and the growth parameters of the nodal segments were examined.Nodal explants formed callus when they were supplemented with 2 mg/L of 6-Benzylaminopurine(BAP).The superior hormonal concentration of Murashige and Skoog(MS)media for the proliferation of shoots from callus cultures(39.33%)was 5 mg/L of 2-Isopentenyl adenine(2iP),and the favorable media for the growth of L.angustifolia callus cultures was 1 and 2 mg/L of BAP,with a 98%for-mation rate in each case.The callus cultures and in vitro propagated L.angustifolia seedlings obtained from var-ious PGR concentrations of MS media were exposed to qualitative and quantitative analysis in terms of phenolic profiles,flavonoids,High-performance liquid chromatography(HPLC)analysis of phenolic acids,and headspace-SPME analysis for volatiles.Such analysis revealed that micropropagated seedlings grown in media containing 1 mg/L of 6-Furfurylaminopurine(KIN)accumulated the highest yield(11.95±0.01 mg GAE/g)of phenolic acids.In contrast,the lowest concentration(2.17±0.04 mg GAE/g)was detected in 0.5 mg/L of BAP+0.5 mg/L of Naphthaleneacetic acid(NAA)media.The plantlets grown in 0.5 mg/L of BAP+0.5 mg/L of NAA media showed the highestflavonoid yield(31.67±0.06μg/g QE/g).In contrast,callus samples exhibited the lowest yield(11.59±0.02μg/g QE/g)offlavonoids in MS media supplemented with a concentration of 0.5 mg/L of BAP.HPLC analysis revealed the variability of phenolic acid contents within the callus cultures as well as plantlets,with gallic acid,4-OH benzoic acid,chlorogenic acid,vanillic acid,caffeic acid,cinnamic acid,and rosmarinic acid being the prominent constituents.The presence of twenty-two chemicals was revealed by headspace-SPME analysis.Eucalyptol,nonanal,borneol,carvone,andβ-caryophyllene were the most abundant.This study demonstrated that micropropagation of L.angustifolia may be an effective method to produce large numbers of genetically identical plantlets for the production of high-value bio compounds.展开更多
In order to decisively determine the adsorption selectivity of zirconium MOF(UiO-66) towards anionic versus cationic species, the adsorptive removal of the anionic dyes(Alizarin Red S.(ARS), Eosin(E), Fuchsin Acid(FA)...In order to decisively determine the adsorption selectivity of zirconium MOF(UiO-66) towards anionic versus cationic species, the adsorptive removal of the anionic dyes(Alizarin Red S.(ARS), Eosin(E), Fuchsin Acid(FA)and Methyl Orange(MO)) and the cationic dyes(Neutral Red(NR), Fuchsin Basic(FB), Methylene Blue(MB),and Safranine T(ST)) has been evaluated. The results clearly reveal a significant selectivity towards anionic dyes. Such an observation agrees with a plethora of reports of UiO-66 superior affinity towards other anionic species(Floride, PO_4^(3-), Diclofenac sodium, Methylchlorophenoxy-propionic acid, Phenols, CrO_4^(2-), SeO_3^(2-), and AsO_4^-). The adsorption process of ARS as an example has been optimized using the central composite design(CCD). The resultant statistical model indicates a crucial effect of both pH and sorbent mass. The optimum conditions were determined to be initial dye concentration 11.82 mg.L^(-1), adsorbent amount 0.0248 g, shaking time of 36 min and pH 2. The adsorption process proceeds via pseudo-second order kinetics(R^2= 0.999). The equilibrium data were fit to Langmuir and Tempkin models(R^2= 0.999 and 0.997 respectively). The results reveal an exceptional removal for the anionic dye(Alizarin Red S.) with a record adsorption capacity of400 mg·g^(-1). The significantly high adsorption capacity of UiO-66 towards ARS adds further evidence to the recently reported exceptional performance of MOFs in pollutants removal from water.展开更多
Based on the characteristics of the deep circular tunnel, the surrounding rock was divided into three regions： the cracked region, the plastic region and the viscoelastic region. The process of rock stress deformatio...Based on the characteristics of the deep circular tunnel, the surrounding rock was divided into three regions： the cracked region, the plastic region and the viscoelastic region. The process of rock stress deformation and change was divided into three stages after the roadway excavation. By using the elastic-plastic mechanics theory, the analytical solutions of the surrounding stress and displacement at different stages and the radii of cracked and plastic regions were formulated. We additionally explained the surrounding rock stress and displacement which appeared in practical project. Simultaneously, based on the problem which emerged from a mine in Xuzhou during the excavating process of rock roadway＇s transport, we got the theoretical solutions for the stress and displacement in the process of rock roadway＇s excavation and considered that the broken area of rock roadway was largely loosing circle. The results indicate that according to the rheological characteristics of surrounding rock, in the primeval excavation of rock roadway, we should increase the length of anchor bolt and cooperate it with anchor nets cable-U steel supporting frame. In addition, when the deformation rate of the surrounding rock is descending after the 15 days＇ excavation, we should use the ＂three anchor＂ supporting method （anchor bolt spray, anchor note and anchor rove） and set aside about 20 cm as the reserved deformation laver.展开更多
[Objective] The aim was to discuss the effects of probiotics on the growth of Cyprinus carpiod and water quality. [Method] Taking C. carpiod as the research object, probiotics were supplemented in the fodder and water...[Objective] The aim was to discuss the effects of probiotics on the growth of Cyprinus carpiod and water quality. [Method] Taking C. carpiod as the research object, probiotics were supplemented in the fodder and water to study their effects on the growth of C. carpiod and water quality. [Result] Probiotics had promoting effects on the growth of C. carpiod and its optimum dosage was 6%. pH, ammonia nitrogen content and nitrite content in water body in experimental groups were all lower than those in control group. [Conclusion] Compound probiotics had a broad application foreground in the aquatic breeding industry.展开更多
DNA analysis is the core of biotechnology applied in petroleum resources and engineering. Traditionally accurate determination of DNA purity and concentration by spectrometer is the first and critical step for downstr...DNA analysis is the core of biotechnology applied in petroleum resources and engineering. Traditionally accurate determination of DNA purity and concentration by spectrometer is the first and critical step for downstream molecular biology research. In this study, three different spectrophotometry methods, BPM, NDTT and NPMTTZ were compared for their performance in determining DNA concentration and purity in 32 oil samples, and molecule methods like quantitative real-time PCR (qPCR) and high-throughput sequence were also performed to help assess the accuracy of the three methods in determining DNA concentration and purity. For ordinary heavy oil (OHO), extra heavy oil (EHO) and super heavy oil (SHO), the characteristics of high viscosity (η), density (ρ) and resin plus asphaltene content will affect the DNA extraction and UV determination. The DNA concentration was decreased as density increased: OHO (11.46 ± 18.34 ng/μL), EHO (6.68 ± 9.67 ng/μL) and SHO (6.20 ± 7.83 ng/μL), and the DNA purity was on the reverse: OHO (1.31 ± 0.27), EHO (1.54 ± 0.20), and SHO (1.83 ± 0.32). The results suggest that spectrophotometry such as BPM and NPMTTZ are qualitatively favorite methods as the quick non-consumable methods in determining DNA concentration and purity of medium oil and heavy oil.展开更多
The α-Al_2O_3 platelets were prepared via solid-state reactions and the effect of the amount of lithium nitrate additive on the property of the platelets was investigated. The ICP results indicated that the high temp...The α-Al_2O_3 platelets were prepared via solid-state reactions and the effect of the amount of lithium nitrate additive on the property of the platelets was investigated. The ICP results indicated that the high temperature calcination process resulted in a large loss of lithium species because of volatilization, but there was still a small amount of residual lithium species in the α-Al_2O_3 platelets. The SEM micrographs showed that lithium nitrate led to decrease in the thickness of α-Al_2O_3 platelets and irregular morphology of aggregates. Pore structures results exhibited that addition of lithium nitrate led to decrease in the pore size and increase in the specific surface area of aggregates of α-Al_2O_3 platelets. The XRD and IR patterns suggested that the residual lithium and aluminum oxide formed LiAl_5O_8. The existence of LiAl_5O_8 was the basic reason for the changed performance of α-Al_2O_3 platelets.展开更多
Black liquor is obtained as a by-product of the pulping process,which is used to convert biomass into pulp by removing lignin,hemicelluloses and other extractives from wood to free cellulose fibers.Lignin represents a...Black liquor is obtained as a by-product of the pulping process,which is used to convert biomass into pulp by removing lignin,hemicelluloses and other extractives from wood to free cellulose fibers.Lignin represents a major constituent in black liquor,with quantities varying from 20%to 30%,of which a very low share is used for manufacturing value-added products,while the rest is mainly burned for energy purposes,thus underestimating its great potential as a raw material.Therefore,it is essential to establish new isolation and extraction methods to increase lignin valorization in the development of bio-based chemicals.The aim of this research work was to determine the effect of KOH or ethanol concentration as an isolation agent on lignin yields and the chemical characteristics of lignin isolated from formacell black liquor of oil palm empty fruit bunch(OPEFB).Isolation of lignin was carried out using KOH with various concentrations ranging from 5%to 15%(w/v).Ethanol was also used to precipitate lignin from black liquor at concentrations varying from 5%to 30%(v/v).The results obtained showed that the addition of KOH solution at 12.5%and 15%concentrations resulted in better lignin yield and chemical properties of lignin,i.e.,pH values of 3.86 and 4.27,lignin yield of 12.78%and 14.95%,methoxyl content of 11.33%and 10.13%,and lignin equivalent weights of 476.25 and 427.03,respectively.Due to its phenolic structure and rich functional groups that are favorable for modifications,lignin has the potential to be used as a green additive in the development of advanced biocomposite products in various applications to replace current fossil fuel-based material,ranging from fillers,fire retardants,formaldehyde scavengers,carbon fibers,aerogels,and wood adhesives.展开更多
1 Introduction In 2006,plankton investigation was carried out in the lakes in Ali district,Tibet.7 planktonic crustaceans were identified and reported.Metadiaptomus asiaticus Uljanirs was reported for the first time o...1 Introduction In 2006,plankton investigation was carried out in the lakes in Ali district,Tibet.7 planktonic crustaceans were identified and reported.Metadiaptomus asiaticus Uljanirs was reported for the first time on the QTP(Yuan et al.,2007).However,detailed data on this species,especially its ecological significance,were not mentioned.In this展开更多
In this article, the results obtained from a study carried out on the some elements-incorporated diamond-like carbon (DLC) films are reported. All the films were deposited using plasma-based ion implantation (PBII) te...In this article, the results obtained from a study carried out on the some elements-incorporated diamond-like carbon (DLC) films are reported. All the films were deposited using plasma-based ion implantation (PBII) technique. The deposited films were annealed at 400℃, 650℃ and 900℃ in an air atmosphere for 1 hour. The effects of adding hydrogen, silicon/oxygen and silicon/nitrogen into the DLC film on chemical composition, friction coefficient and corrosion resistance were investigated. The films coated micro end mills performance was also assessed. The results indicate that all the films showed almost constant atomic contents of C, Si, O and N until annealing at 400℃. However, the films were completely destroyed at 650℃ with the increased Si and O contents, while the C content decreased. The incorporation of silicon/oxygen and silicon/nitrogen into the DLC exhibited lower values of friction coefficients than the hydrogenated DLC (DLC and H-DLC) before and after annealing at 400℃, whereas all the films presented the same values of friction coefficients after annealing at 650℃ due to the completely destroy of the films. Furthermore, the incorporation of silicon/nitrogen into the DLC also exhibited better corrosion resistance and unbroken micro end mills performance on their surfaces. Thus, the incorporation of silicon/nitrogen into the DLC film can be considered beneficial in improving the micro end mills performance.展开更多
This experiment aimed to determine how Cd exposure impacts CD4 cell activation,macrophage cells,pinocytosis activity,metallothionine expression,and Cd levels in juvenile Gambusia affinis gills.Four treatment groups we...This experiment aimed to determine how Cd exposure impacts CD4 cell activation,macrophage cells,pinocytosis activity,metallothionine expression,and Cd levels in juvenile Gambusia affinis gills.Four treatment groups were used,with one control and treatments A,B,C,and D at dosages of 0.03 mg/L,0.023 mg/L,0.015 mg/L,and 0.008 mg/L,respectively.The results showed that the number of CD4 with CD8 cell markers differed significantly from each treatment compared to the control(0.33%).The value in A,namely 0.54%,was the highest,followed by B,C,and D of 0.46%,0.44%,and 0.42%,respectively.The number of macrophages increased as the Cd level of the medium increased,as did the activity of pinocytosis.Furthermore,the immunofluorescence test on the gills with the Anti-MT Mouse and goat IgG fluoresce in Rhodamine on the gills showed that luminescence increased with increasing Cd levels in the gills.Similarly,the MT intensity increased at Cd-exposed gills as compared to the control.As the Cd level in the gills increased,the MT levels ascended significantly.展开更多
基金supported by the Inner Mongolia Power Company 2024 Staff Innovation Studio Innovation Project“Research on Cluster Output Prediction and Group Control Technology for County-Wide Distributed Photovoltaic Construction”.
文摘Responding to the stochasticity and uncertainty in the power height of distributed photovoltaic power generation.This paper presents a distributed photovoltaic ultra-short-term power forecasting method based on Variational Mode Decomposition(VMD)and Channel Attention Mechanism.First,Pearson’s correlation coefficient was utilized to filter out the meteorological factors that had a high impact on historical power.Second,the distributed PV power data were decomposed into a relatively smooth power series with different fluctuation patterns using variational modal decomposition(VMD).Finally,the reconstructed distributed PV power as well as other features are input into the combined CNN-SENet-BiLSTM model.In this model,the convolutional neural network(CNN)and channel attention mechanism dynamically adjust the weights while capturing the spatial features of the input data to improve the discriminative ability of key features.The extracted data is then fed into the bidirectional long short-term memory network(BiLSTM)to capture the time-series features,and the final output is the prediction result.The verification is conducted using a dataset from a distributed photovoltaic power station in the Northwest region of China.The results show that compared with other prediction methods,the method proposed in this paper has a higher prediction accuracy,which helps to improve the proportion of distributed PV access to the grid,and can guarantee the safe and stable operation of the power grid.
基金the National Key R&D Program of China(No.2022YFC2904103)the Key Program of the National Natural Science Foundation of China(No.52034001)+1 种基金the 111 Project(No.B20041)the China National Postdoctoral Program for Innovative Talents(No.BX20230041)。
文摘Traditional research believes that the filling body can effectively control stress concentration while ignoring the problems of unknown stability and the complex and changeable stress distribution of the filling body–surrounding rock combination under high-stress conditions.Current monitoring data processing methods cannot fully consider the complexity of monitoring objects,the diversity of monitoring methods,and the dynamics of monitoring data.To solve this problem,this paper proposes a phase space reconstruction and stability prediction method to process heterogeneous information of backfill–surrounding rock combinations.The three-dimensional monitoring system of a large-area filling body–surrounding rock combination in Longshou Mine was constructed by using drilling stress,multipoint displacement meter,and inclinometer.Varied information,such as the stress and displacement of the filling body–surrounding rock combination,was continuously obtained.Combined with the average mutual information method and the false nearest neighbor point method,the phase space of the heterogeneous information of the filling body–surrounding rock combination was then constructed.In this paper,the distance between the phase point and its nearest point was used as the index evaluation distance to evaluate the stability of the filling body–surrounding rock combination.The evaluated distances(ED)revealed a high sensitivity to the stability of the filling body–surrounding rock combination.The new method was then applied to calculate the time series of historically ED for 12 measuring points located at Longshou Mine.The moments of mutation in these time series were at least 3 months ahead of the roadway return dates.In the ED prediction experiments,the autoregressive integrated moving average model showed a higher prediction accuracy than the deep learning models(long short-term memory and Transformer).Furthermore,the root-mean-square error distribution of the prediction results peaked at 0.26,thus outperforming the no-prediction method in 70%of the cases.
文摘An effective in vitro propagation protocol was designed for Lavandula angustifolia Miller,a medicinal aromatic plant that is a prominent source of volatile organic compounds(VOCs).Murashige and Skoog media were supplemented with various concentrations of Plant Growth Regulators(PGRs),and the growth parameters of the nodal segments were examined.Nodal explants formed callus when they were supplemented with 2 mg/L of 6-Benzylaminopurine(BAP).The superior hormonal concentration of Murashige and Skoog(MS)media for the proliferation of shoots from callus cultures(39.33%)was 5 mg/L of 2-Isopentenyl adenine(2iP),and the favorable media for the growth of L.angustifolia callus cultures was 1 and 2 mg/L of BAP,with a 98%for-mation rate in each case.The callus cultures and in vitro propagated L.angustifolia seedlings obtained from var-ious PGR concentrations of MS media were exposed to qualitative and quantitative analysis in terms of phenolic profiles,flavonoids,High-performance liquid chromatography(HPLC)analysis of phenolic acids,and headspace-SPME analysis for volatiles.Such analysis revealed that micropropagated seedlings grown in media containing 1 mg/L of 6-Furfurylaminopurine(KIN)accumulated the highest yield(11.95±0.01 mg GAE/g)of phenolic acids.In contrast,the lowest concentration(2.17±0.04 mg GAE/g)was detected in 0.5 mg/L of BAP+0.5 mg/L of Naphthaleneacetic acid(NAA)media.The plantlets grown in 0.5 mg/L of BAP+0.5 mg/L of NAA media showed the highestflavonoid yield(31.67±0.06μg/g QE/g).In contrast,callus samples exhibited the lowest yield(11.59±0.02μg/g QE/g)offlavonoids in MS media supplemented with a concentration of 0.5 mg/L of BAP.HPLC analysis revealed the variability of phenolic acid contents within the callus cultures as well as plantlets,with gallic acid,4-OH benzoic acid,chlorogenic acid,vanillic acid,caffeic acid,cinnamic acid,and rosmarinic acid being the prominent constituents.The presence of twenty-two chemicals was revealed by headspace-SPME analysis.Eucalyptol,nonanal,borneol,carvone,andβ-caryophyllene were the most abundant.This study demonstrated that micropropagation of L.angustifolia may be an effective method to produce large numbers of genetically identical plantlets for the production of high-value bio compounds.
文摘In order to decisively determine the adsorption selectivity of zirconium MOF(UiO-66) towards anionic versus cationic species, the adsorptive removal of the anionic dyes(Alizarin Red S.(ARS), Eosin(E), Fuchsin Acid(FA)and Methyl Orange(MO)) and the cationic dyes(Neutral Red(NR), Fuchsin Basic(FB), Methylene Blue(MB),and Safranine T(ST)) has been evaluated. The results clearly reveal a significant selectivity towards anionic dyes. Such an observation agrees with a plethora of reports of UiO-66 superior affinity towards other anionic species(Floride, PO_4^(3-), Diclofenac sodium, Methylchlorophenoxy-propionic acid, Phenols, CrO_4^(2-), SeO_3^(2-), and AsO_4^-). The adsorption process of ARS as an example has been optimized using the central composite design(CCD). The resultant statistical model indicates a crucial effect of both pH and sorbent mass. The optimum conditions were determined to be initial dye concentration 11.82 mg.L^(-1), adsorbent amount 0.0248 g, shaking time of 36 min and pH 2. The adsorption process proceeds via pseudo-second order kinetics(R^2= 0.999). The equilibrium data were fit to Langmuir and Tempkin models(R^2= 0.999 and 0.997 respectively). The results reveal an exceptional removal for the anionic dye(Alizarin Red S.) with a record adsorption capacity of400 mg·g^(-1). The significantly high adsorption capacity of UiO-66 towards ARS adds further evidence to the recently reported exceptional performance of MOFs in pollutants removal from water.
基金provided by the National Basic Research Program of China (No. 2007CB209408)the National Natural Science Foundation of China (No. 51074166)the Research Innovation Program for College Graduates of Jiangsu (No.CX098B_108Z)
文摘Based on the characteristics of the deep circular tunnel, the surrounding rock was divided into three regions： the cracked region, the plastic region and the viscoelastic region. The process of rock stress deformation and change was divided into three stages after the roadway excavation. By using the elastic-plastic mechanics theory, the analytical solutions of the surrounding stress and displacement at different stages and the radii of cracked and plastic regions were formulated. We additionally explained the surrounding rock stress and displacement which appeared in practical project. Simultaneously, based on the problem which emerged from a mine in Xuzhou during the excavating process of rock roadway＇s transport, we got the theoretical solutions for the stress and displacement in the process of rock roadway＇s excavation and considered that the broken area of rock roadway was largely loosing circle. The results indicate that according to the rheological characteristics of surrounding rock, in the primeval excavation of rock roadway, we should increase the length of anchor bolt and cooperate it with anchor nets cable-U steel supporting frame. In addition, when the deformation rate of the surrounding rock is descending after the 15 days＇ excavation, we should use the ＂three anchor＂ supporting method （anchor bolt spray, anchor note and anchor rove） and set aside about 20 cm as the reserved deformation laver.
基金Supported by the Project of Quality Safety of Agricultural Products Loaned from World Bank of Jilin Province
文摘[Objective] The aim was to discuss the effects of probiotics on the growth of Cyprinus carpiod and water quality. [Method] Taking C. carpiod as the research object, probiotics were supplemented in the fodder and water to study their effects on the growth of C. carpiod and water quality. [Result] Probiotics had promoting effects on the growth of C. carpiod and its optimum dosage was 6%. pH, ammonia nitrogen content and nitrite content in water body in experimental groups were all lower than those in control group. [Conclusion] Compound probiotics had a broad application foreground in the aquatic breeding industry.
基金supported by grants from the PetroChina-CUP Major Strategic Cooperation Projects(ZLZX2020010805,ZLZX2020020405)National Natural Science Foundation of China(41373086)+3 种基金National Science and Technology Major Project(No.2016ZX05050011,2016ZX05040002)Beijing Nova Program and Leading Talent Culturing Cooperative Projects(No.Z161100004916033)Beijing Higher Education Young Elite Teacher Project(No.YETP0670)Outstanding Young Excellent Teachers Foundation of China University of Petroleum(Beijing)(KYJJ2012-01-10).
文摘DNA analysis is the core of biotechnology applied in petroleum resources and engineering. Traditionally accurate determination of DNA purity and concentration by spectrometer is the first and critical step for downstream molecular biology research. In this study, three different spectrophotometry methods, BPM, NDTT and NPMTTZ were compared for their performance in determining DNA concentration and purity in 32 oil samples, and molecule methods like quantitative real-time PCR (qPCR) and high-throughput sequence were also performed to help assess the accuracy of the three methods in determining DNA concentration and purity. For ordinary heavy oil (OHO), extra heavy oil (EHO) and super heavy oil (SHO), the characteristics of high viscosity (η), density (ρ) and resin plus asphaltene content will affect the DNA extraction and UV determination. The DNA concentration was decreased as density increased: OHO (11.46 ± 18.34 ng/μL), EHO (6.68 ± 9.67 ng/μL) and SHO (6.20 ± 7.83 ng/μL), and the DNA purity was on the reverse: OHO (1.31 ± 0.27), EHO (1.54 ± 0.20), and SHO (1.83 ± 0.32). The results suggest that spectrophotometry such as BPM and NPMTTZ are qualitatively favorite methods as the quick non-consumable methods in determining DNA concentration and purity of medium oil and heavy oil.
基金supported by the Technology Development (Commission) Project of SINOPEC Catalyst Co. Ltd. (Grant No. 14-05-01)
文摘The α-Al_2O_3 platelets were prepared via solid-state reactions and the effect of the amount of lithium nitrate additive on the property of the platelets was investigated. The ICP results indicated that the high temperature calcination process resulted in a large loss of lithium species because of volatilization, but there was still a small amount of residual lithium species in the α-Al_2O_3 platelets. The SEM micrographs showed that lithium nitrate led to decrease in the thickness of α-Al_2O_3 platelets and irregular morphology of aggregates. Pore structures results exhibited that addition of lithium nitrate led to decrease in the pore size and increase in the specific surface area of aggregates of α-Al_2O_3 platelets. The XRD and IR patterns suggested that the residual lithium and aluminum oxide formed LiAl_5O_8. The existence of LiAl_5O_8 was the basic reason for the changed performance of α-Al_2O_3 platelets.
基金This work was also supported by the Project“Development,Properties,and Application of Eco-Friendly Wood-Based Composites”,No.HИC-Б-1145/04.2021,carried out at the University of Forestry,Sofia,Bulgaria.The authors would like to acknowledge the Fundamental Research Grant Scheme(FRGS 2018-1)Reference Code:FRGS/1/2018/WAB07/UPM/1 provided by the Ministry of Higher Education,Malaysia.
文摘Black liquor is obtained as a by-product of the pulping process,which is used to convert biomass into pulp by removing lignin,hemicelluloses and other extractives from wood to free cellulose fibers.Lignin represents a major constituent in black liquor,with quantities varying from 20%to 30%,of which a very low share is used for manufacturing value-added products,while the rest is mainly burned for energy purposes,thus underestimating its great potential as a raw material.Therefore,it is essential to establish new isolation and extraction methods to increase lignin valorization in the development of bio-based chemicals.The aim of this research work was to determine the effect of KOH or ethanol concentration as an isolation agent on lignin yields and the chemical characteristics of lignin isolated from formacell black liquor of oil palm empty fruit bunch(OPEFB).Isolation of lignin was carried out using KOH with various concentrations ranging from 5%to 15%(w/v).Ethanol was also used to precipitate lignin from black liquor at concentrations varying from 5%to 30%(v/v).The results obtained showed that the addition of KOH solution at 12.5%and 15%concentrations resulted in better lignin yield and chemical properties of lignin,i.e.,pH values of 3.86 and 4.27,lignin yield of 12.78%and 14.95%,methoxyl content of 11.33%and 10.13%,and lignin equivalent weights of 476.25 and 427.03,respectively.Due to its phenolic structure and rich functional groups that are favorable for modifications,lignin has the potential to be used as a green additive in the development of advanced biocomposite products in various applications to replace current fossil fuel-based material,ranging from fillers,fire retardants,formaldehyde scavengers,carbon fibers,aerogels,and wood adhesives.
基金supported by the National Natural Science Foundation of China (41372179)the Special Funds for Public Welfare Land and Resour ces Scientific Project ( 201311140)
文摘1 Introduction In 2006,plankton investigation was carried out in the lakes in Ali district,Tibet.7 planktonic crustaceans were identified and reported.Metadiaptomus asiaticus Uljanirs was reported for the first time on the QTP(Yuan et al.,2007).However,detailed data on this species,especially its ecological significance,were not mentioned.In this
基金Acknowledgements： The work is supported by National Natural Science Foundation of China （No. 50675047） and Samsung Semiconductor （China） R ＆ D Co., Ltd. The authors would like to thank Professor LI Ming-yu in Shenzhen Graduate School, Harbin Institute of Technology, and Dr. DING Ying, in Microjoining Lab, Schoo） of Materials Science and Engineering, Harbin Institute of Technology for their comments.
文摘In this article, the results obtained from a study carried out on the some elements-incorporated diamond-like carbon (DLC) films are reported. All the films were deposited using plasma-based ion implantation (PBII) technique. The deposited films were annealed at 400℃, 650℃ and 900℃ in an air atmosphere for 1 hour. The effects of adding hydrogen, silicon/oxygen and silicon/nitrogen into the DLC film on chemical composition, friction coefficient and corrosion resistance were investigated. The films coated micro end mills performance was also assessed. The results indicate that all the films showed almost constant atomic contents of C, Si, O and N until annealing at 400℃. However, the films were completely destroyed at 650℃ with the increased Si and O contents, while the C content decreased. The incorporation of silicon/oxygen and silicon/nitrogen into the DLC exhibited lower values of friction coefficients than the hydrogenated DLC (DLC and H-DLC) before and after annealing at 400℃, whereas all the films presented the same values of friction coefficients after annealing at 650℃ due to the completely destroy of the films. Furthermore, the incorporation of silicon/nitrogen into the DLC also exhibited better corrosion resistance and unbroken micro end mills performance on their surfaces. Thus, the incorporation of silicon/nitrogen into the DLC film can be considered beneficial in improving the micro end mills performance.
基金Ministry Education and Culture,Directorate of Research and Technology for Research and Innovation,grant number 028/AMD-SP2H/LT-MULTI-PDPK/LL7/2021.
文摘This experiment aimed to determine how Cd exposure impacts CD4 cell activation,macrophage cells,pinocytosis activity,metallothionine expression,and Cd levels in juvenile Gambusia affinis gills.Four treatment groups were used,with one control and treatments A,B,C,and D at dosages of 0.03 mg/L,0.023 mg/L,0.015 mg/L,and 0.008 mg/L,respectively.The results showed that the number of CD4 with CD8 cell markers differed significantly from each treatment compared to the control(0.33%).The value in A,namely 0.54%,was the highest,followed by B,C,and D of 0.46%,0.44%,and 0.42%,respectively.The number of macrophages increased as the Cd level of the medium increased,as did the activity of pinocytosis.Furthermore,the immunofluorescence test on the gills with the Anti-MT Mouse and goat IgG fluoresce in Rhodamine on the gills showed that luminescence increased with increasing Cd levels in the gills.Similarly,the MT intensity increased at Cd-exposed gills as compared to the control.As the Cd level in the gills increased,the MT levels ascended significantly.
