As the main component of electronic products,plastics contain complex and diverse metal additives.Recycling process is not conducive to stable existence of metal additives in electronic plastics.Once the e-waste plast...As the main component of electronic products,plastics contain complex and diverse metal additives.Recycling process is not conducive to stable existence of metal additives in electronic plastics.Once the e-waste plastics enter the environment,they will continue to release harmful metals into environment after aging,causing serious hazards.This study delved into the analysis and comparison of metal content of e-waste plastics,elucidating aging process and metal leaching behavior over a 112-day natural light exposure period.The findings underscored that metal content in recycled plastics surpassed that in their new counterparts.Specifically,Ti content in new plastics remained below 100 mg/kg,while recycled plastics exhibited Ti content surpassing 100 mg/kg threshold.Throughout prolonged natural light exposure,metals such as Zn,Ba and Sb demonstrated a heightened likelihood of release from electronic plastics in comparison to other metals.The aging process during light exposure led to fragmentation of electronic plastics,accompanied by a reduction in particle size.Notably,the particle size reduction was more pronounced in poly acrylonitrile butadiene styrene(ABS)and recycled ABS,experiencing reductions of 40µm and 85µm,respectively.This phenomenon was attributed to the presence of polybutadiene structural units,which proved more susceptible to aging.Along with the breaking of plastics,the ABS plastics released metal species such as Pb,Cd,Ni,Al that had not been detected in other plastics solutions.The collective evidence from this study suggested that ABS and recycled ABS electronic plastics might pose a heightened potential environmental risk compared to other electronic plastics.展开更多
The simultaneous sorption behavior and characteristics of cadmium (Cd) and sulfamethoxazole (SMX) on rice straw biochar were investigated. Isotherms of Cd and SMX were well modeled by the Langmuir equation (R2 >0.9...The simultaneous sorption behavior and characteristics of cadmium (Cd) and sulfamethoxazole (SMX) on rice straw biochar were investigated. Isotherms of Cd and SMX were well modeled by the Langmuir equation (R2 >0.95). The calculated maximum adsorption parameter (Q) of Cd was similar in single and binary systems (34129.69 and 35919.54 mg/kg, respectively). However, the Q of SMX in a binary system (9182.74 mg/kg) was much higher than that in a single system (1827.82 mg/kg). The presence of Cd significantly promoted the sorption of SMX on rice straw biochar. When the pH ranged from 3 to 7.5, the sorption of Cd had the characteristics of a parabola pattern with maximum adsorption at pH 5, while the adsorption quantity of SMX decreased with increasing pH, with maximum adsorption at pH 3. The amount of SMX adsorbed on biochar was positively correlated with the surface area of the biochar, and the maximum adsorption occurred with d 250 biochar (biochar with a diameter of 150-250 μm). Scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR) showed that the removal of Cd and SMX by rice straw biochar may be attributed to precipitation and the formation of surface complexes between Cd or SMX and carboxyl or hydroxyl groups. The results of this study indicate that rice straw biochar has the potential for simultaneous removal of Cd and SMX from co-contaminated water.展开更多
In order to investigate the behavior characteristics of asphalt components during the process of nano-cracks temperature self-healing(NTS),molecular simulation technology was used to simulate the temperature self-heal...In order to investigate the behavior characteristics of asphalt components during the process of nano-cracks temperature self-healing(NTS),molecular simulation technology was used to simulate the temperature self-healing of asphalt.Based on the determination of asphalts(virgin asphalt and aged asphalt),the proportional changes of asphalt components were confirmed.The distribution characteristics of asphalt components were obtained by marking different components in the process of NTS.At the same time,the rationality of the micro simulation findings was confirmed by asphalt performance tests and infrared spectroscopy results.The results show that different asphalt components have different behavior characteristics in the process of NTS.The content of asphaltene and resin plays a key role in the process of NTS,while saturation and aromatics play an active role in the temperature self-healing before and after aging.The NTS is the result of the comprehensive action of different components,and the self-healing efficiency is proportional to the relative molecular mass.The effect of aging on the NTS is also realized by changing the proportion of asphalt components.展开更多
It is known that large amounts of residual lignin are generated in the pulp and paper industry.A new alternative for Kraft lignin valorization,which consists of first a chemical modification using a diisocyanate and t...It is known that large amounts of residual lignin are generated in the pulp and paper industry.A new alternative for Kraft lignin valorization,which consists of first a chemical modification using a diisocyanate and then the efficient dispersion in castor oil to achieve stable gel-like systems,is proposed in this work.Rheological properties and microstructure of these materials were determined by means of small amplitude oscillatory shear tests and viscous flow measurements and atomic force microscopy observations,respectively.Moreover,both standardized penetration tests and tribological assays,usually performed in the lubricant industry,were carried out to evaluate the performance characteristics as lubricating greases.Linear viscoelasticity functions are affected by the lignin/diisocyanate ratio and thickener concentration.The thermorheological response evidenced a softening temperature of around 105°C.The microstructure of these gel-like dispersions is composed of interconnected thin fibers,homogeneously distributed in castor oil.Moreover,the NCO-functionalized lignin gel-like dispersions studied show lower friction coefficients than traditional lubricating greases.展开更多
Modified polyimides(MPIs)show great potential towards 5G communication applications,due to its excellent thermal stability,mechanical property and chemical stability as compared to most of polymers.Introducing fluorid...Modified polyimides(MPIs)show great potential towards 5G communication applications,due to its excellent thermal stability,mechanical property and chemical stability as compared to most of polymers.Introducing fluoride groups or porous structure is favorable to ultra-low dielectric constant(D_(k))and dielectric loss(D_(f)).However,the cost of the fluorinated MPIs is high and their synthetic processes are complicated,and porous MPIs suffer poor mechanical properties.Also,increasing the fraction of free volume is a very effective way to lower D_(k)through introducing more ultra-low-D_(k)air component.However,most of this kind of MPIs lag far behind the fluorinated MPIs and the porous MPIs in terms of ultra-low D_(f),hindering the application of MPIs in high-speed communication devices.Thus,it is highly desirable to develop intrinsic ultra-low-D_(k)/D_(f)MPIs at high frequency with less fluoric groups and nonporous structure.Herein,we introduce a facile and effective strategy to lower D_(k)and D_(f)through introducing rigid and large sterically hindered aromatic groups into MPIs.On the one hand,their large steric hindrance effect leads to low D_(k)by increasing intrinsic free volume.On the other hand,the resulting highly stiff polymer chain and strong intermolecular interaction are favorable to reduce D_(f)by inhibiting dipole orientations.Based on this strategy,the spirobifluorene groups are preferred.The as-prepared MPIs show excellent dielectric performance with low D_(k)of 2.74–2.76 and low D_(f)of 0.00599 at 10 GHz,to some extent,exceeding the multiple fluorinated MPI with D_(k)/D_(f)of 2.67/0.00663 at 10 GHz.展开更多
In order to further study the reliability of macro evaluation indexes,molecular dynamics (MD) was applied to the evaluation of asphalt binder.Micro evaluation indexes (potential energy,surface free energy,solubility p...In order to further study the reliability of macro evaluation indexes,molecular dynamics (MD) was applied to the evaluation of asphalt binder.Micro evaluation indexes (potential energy,surface free energy,solubility parameter and diffusion coefficient) of asphalt binder in different service phases (virgin,modified,aged and rejuvenated) were simulated.Combined with the variation characteristics of asphalt binder macro evaluation indexes (permeability,ductility,viscosity and softening point) in different service phases,the cross-scale correlation of macro-micro evaluation indexes was explored.The results show that the macro and micro evaluation indexes of asphalt binder have different characteristics in different service phases.The essence of the variation in the properties of asphalt binders is the difference in micro composition.In addition,there is a certain correlation between macro and micro evaluation indexes,which can be described by the gray relation theory.The cross-scale correlation of macro-micro evaluation indexes can provide a certain theoretical basis for the development of asphalt binder.展开更多
Soil salinity greatly impairs plant growth and crop productivity.Rice(Oryza sativa L.)is a salt-sensitive crop.To better understand the molecular mechanisms of salt tolerance in roots,the BGISEQ-500 sequencing platfor...Soil salinity greatly impairs plant growth and crop productivity.Rice(Oryza sativa L.)is a salt-sensitive crop.To better understand the molecular mechanisms of salt tolerance in roots,the BGISEQ-500 sequencing platform was employed to elucidate transcriptome changes in rice roots after 0,3,24,and 72 h of salt stress.The results showed that root K+content decreased and Na+content increased rapidly after the initial stage of salt stress,but that fresh and dry weight in root did not significantly reduce.Compared to the control(no salt stress),1,292,453,and 486 differentially expressed genes(DEGs)were upregulated,respectively,and 939,894,and 646 DEGs were downregulated,respectively,after 3,24,and 72 h of salt treatment.The number of DEGs was higher during the early stage of salt stress(3 h)than in later stages(24 and 72 h).A number of DEGs involved in the response and adaptation to salt stress were related to protein kinase and calcium-binding,plant hormone signaling and metabolism,transcriptional regulation,metabolic pathways,antioxidant activity,and ion transport.Many of these DEGs were activated during the early stage of salt stress(3 h).The present study reports candidate salt-stressresponsive genes with the potential to genetically improve salt tolerance in rice elsewhere.展开更多
Fermented blueberry wine(BW)contains organic acids that may contribute to gastrointestinal discomfort,but the specific compounds responsible for diarrhea remain unclear.This study aimed to identify the key diarrhea-in...Fermented blueberry wine(BW)contains organic acids that may contribute to gastrointestinal discomfort,but the specific compounds responsible for diarrhea remain unclear.This study aimed to identify the key diarrhea-inducing organic acids by establishing a spectrum-effect relationship between chemical composition and biological activity.Ultra-high performance liquid chromatography(UHPLC)analysis revealed eight major organic acids in BW,with quinic acid predominating.Eight anion-exchange resin pretreatments combined with UHPLC-diode array detector successfully characterized the organic acid profiles of BW.Subsequent studies in mice demonstrated that resin treatment significantly alleviated BW-induced diarrhea in mice,reducing the diarrhea index and attenuating intestinal propulsion.Histopathological and biochemical analyses demonstrated restored intestinal integrity,improved gut barrier function,and normalized serum electrolytes,confirming that organic acids are the causative agents.Further spectrum-effect relationship was established through grey relational,orthogonal partial least squares,and Spearman rank correlation analysis,revealing quinic acid as exhibiting the strongest positive correlation with diarrhea incidence.The findings suggest that quinic acid is a primary contributor to diarrheal effects observed in BW.This study provides critical insights into regulating components in BW production to mitigate adverse gastrointestinal effects and a methodological foundation for material basis research in functional foods.展开更多
Isolation of high-purity milk-derived extracellular vesicles(miEV)is complicated by protein impurities,such as casein.The focus of this study was to investigate whether the treatment of milk with acetic acid(AA),ethyl...Isolation of high-purity milk-derived extracellular vesicles(miEV)is complicated by protein impurities,such as casein.The focus of this study was to investigate whether the treatment of milk with acetic acid(AA),ethylenediaminetetraacetic acid(EDTA),and sodium citrate(SC),which are known chemicals to deplete caseins from miEV,alters their physicochemical and functional characteristics differently.For this purpose,following the treatment of milk with AA,EDTA,and SC,various subpopulations(12 K,35 K,70 K,and 100 K)of miEV were isolated by differential ultracentrifugation and characterized by protein quantification assay,gel electrophoresis,western blotting,dynamic light scattering,and scanning electron microscopy.In addition,the cellular uptake and immunomodulatory potential of miEV were also assessed.The results indicated that protein impurities,including caseins,were differentially depleted from miEV subpopulations by AA,EDTA,and SC.However,the acidification of milk induced partial protein aggregation.Additionally,miEV from both AA-and EDTA-treated milk exhibited a less smooth surface and higher uptake compared to those from SC-treated milk.Although all miEV significantly reduced T cell proliferation,a higher immunomodulatory potential was found in miEV from AA-treated milk.In conclusion,AA,SC,and EDTA have different efficiencies in improving the purity of miEV and can affect their physicochemical and functional characteristics differently.This issue may be critical for the efficient isolation of pure subpopulations of miEV for biomedical research.展开更多
Biochar is a promising technology for carbon storage and greenhouse gas(GHG)reduction,but optimizing it is challenging due to the complexity of natural systems.Machine learning(ML)and natural language processing(NLP)o...Biochar is a promising technology for carbon storage and greenhouse gas(GHG)reduction,but optimizing it is challenging due to the complexity of natural systems.Machine learning(ML)and natural language processing(NLP)offer solutions through enhanced data analysis and pattern recognition,ushering in a new era of biochar research.展开更多
Plasma spray-physical vapor deposition(i.e.,PS-PVD)is a promising method for obtaining advanced environmental barrier coatings(EBCs).The EBCs must meet some requirements in the application,in which the thermal cycle p...Plasma spray-physical vapor deposition(i.e.,PS-PVD)is a promising method for obtaining advanced environmental barrier coatings(EBCs).The EBCs must meet some requirements in the application,in which the thermal cycle performance affects the service lifetime.The preparation of artificial vertical cracks in Yb_(2)SiO_(5) coatings is an effective approach for meeting the requirements above because vertical cracks provide a strain tolerance.To clarify the formation mechanism of vertical cracks during the PSPVD,the effects of coating thickness and substrate temperature on the formation of vertical cracks were investigated.In addition,the interactions of spray powder and plasma flame during coating deposition were also characterized by optical spectroscopy.It is indicated that vertical cracks are formed due to a thermal expansion mismatch between Yb_(2)SiO_(5) and mullite coating,transient cooling after deposition and the nucleation of evaporated Yb_(2)SiO_(5) as well.展开更多
基金the Natural Science Foundation of Guangdong Province(No.2021B1515020041)the National Natural Science Foundation of China(No.42277403)+4 种基金the Projects of International Cooperation and Exchange of the National Natural Science Foundation of China(NSFC-UNEP)(No:32261143459)the Guangdong Provincial Key Laboratory of Soil and Groundwater Pollution Control(No.2023B1212060002)the Guangdong Major Project of Basic and Applied Basic Research(No.2023B0303000024)the High-level University Special Fund(No.G03050K001)the Research Projects of the General Administration of Customs(No.2022HK060)for financial support.
文摘As the main component of electronic products,plastics contain complex and diverse metal additives.Recycling process is not conducive to stable existence of metal additives in electronic plastics.Once the e-waste plastics enter the environment,they will continue to release harmful metals into environment after aging,causing serious hazards.This study delved into the analysis and comparison of metal content of e-waste plastics,elucidating aging process and metal leaching behavior over a 112-day natural light exposure period.The findings underscored that metal content in recycled plastics surpassed that in their new counterparts.Specifically,Ti content in new plastics remained below 100 mg/kg,while recycled plastics exhibited Ti content surpassing 100 mg/kg threshold.Throughout prolonged natural light exposure,metals such as Zn,Ba and Sb demonstrated a heightened likelihood of release from electronic plastics in comparison to other metals.The aging process during light exposure led to fragmentation of electronic plastics,accompanied by a reduction in particle size.Notably,the particle size reduction was more pronounced in poly acrylonitrile butadiene styrene(ABS)and recycled ABS,experiencing reductions of 40µm and 85µm,respectively.This phenomenon was attributed to the presence of polybutadiene structural units,which proved more susceptible to aging.Along with the breaking of plastics,the ABS plastics released metal species such as Pb,Cd,Ni,Al that had not been detected in other plastics solutions.The collective evidence from this study suggested that ABS and recycled ABS electronic plastics might pose a heightened potential environmental risk compared to other electronic plastics.
基金supported by the National Key Technology R&D Program of China (No. 2012BAC17B04)the Fundamental Research Funds for the Central Universities, China
文摘The simultaneous sorption behavior and characteristics of cadmium (Cd) and sulfamethoxazole (SMX) on rice straw biochar were investigated. Isotherms of Cd and SMX were well modeled by the Langmuir equation (R2 >0.95). The calculated maximum adsorption parameter (Q) of Cd was similar in single and binary systems (34129.69 and 35919.54 mg/kg, respectively). However, the Q of SMX in a binary system (9182.74 mg/kg) was much higher than that in a single system (1827.82 mg/kg). The presence of Cd significantly promoted the sorption of SMX on rice straw biochar. When the pH ranged from 3 to 7.5, the sorption of Cd had the characteristics of a parabola pattern with maximum adsorption at pH 5, while the adsorption quantity of SMX decreased with increasing pH, with maximum adsorption at pH 3. The amount of SMX adsorbed on biochar was positively correlated with the surface area of the biochar, and the maximum adsorption occurred with d 250 biochar (biochar with a diameter of 150-250 μm). Scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR) showed that the removal of Cd and SMX by rice straw biochar may be attributed to precipitation and the formation of surface complexes between Cd or SMX and carboxyl or hydroxyl groups. The results of this study indicate that rice straw biochar has the potential for simultaneous removal of Cd and SMX from co-contaminated water.
基金Funded by the Fundamental Research Funds for the Central Universities(No.2572021AW10)。
文摘In order to investigate the behavior characteristics of asphalt components during the process of nano-cracks temperature self-healing(NTS),molecular simulation technology was used to simulate the temperature self-healing of asphalt.Based on the determination of asphalts(virgin asphalt and aged asphalt),the proportional changes of asphalt components were confirmed.The distribution characteristics of asphalt components were obtained by marking different components in the process of NTS.At the same time,the rationality of the micro simulation findings was confirmed by asphalt performance tests and infrared spectroscopy results.The results show that different asphalt components have different behavior characteristics in the process of NTS.The content of asphaltene and resin plays a key role in the process of NTS,while saturation and aromatics play an active role in the temperature self-healing before and after aging.The NTS is the result of the comprehensive action of different components,and the self-healing efficiency is proportional to the relative molecular mass.The effect of aging on the NTS is also realized by changing the proportion of asphalt components.
文摘It is known that large amounts of residual lignin are generated in the pulp and paper industry.A new alternative for Kraft lignin valorization,which consists of first a chemical modification using a diisocyanate and then the efficient dispersion in castor oil to achieve stable gel-like systems,is proposed in this work.Rheological properties and microstructure of these materials were determined by means of small amplitude oscillatory shear tests and viscous flow measurements and atomic force microscopy observations,respectively.Moreover,both standardized penetration tests and tribological assays,usually performed in the lubricant industry,were carried out to evaluate the performance characteristics as lubricating greases.Linear viscoelasticity functions are affected by the lignin/diisocyanate ratio and thickener concentration.The thermorheological response evidenced a softening temperature of around 105°C.The microstructure of these gel-like dispersions is composed of interconnected thin fibers,homogeneously distributed in castor oil.Moreover,the NCO-functionalized lignin gel-like dispersions studied show lower friction coefficients than traditional lubricating greases.
基金Key-Area Research and Development Program of Guangdong Province(No.2020B010182001)the National Natural Science Foundation of China(Nos.U20A20340 and 52001068)+3 种基金National Key R&D Program of China(No.2020YFB0408100)Guangdong Innovative and Entrepreneurial Research Team Program(No.2016ZT06C412)the“One-Hundred Young Talents”of Guangdong University of Technology(No.220413236)Foshan Introducing Innovative and Entrepreneurial Teams(No.1920001000108)。
文摘Modified polyimides(MPIs)show great potential towards 5G communication applications,due to its excellent thermal stability,mechanical property and chemical stability as compared to most of polymers.Introducing fluoride groups or porous structure is favorable to ultra-low dielectric constant(D_(k))and dielectric loss(D_(f)).However,the cost of the fluorinated MPIs is high and their synthetic processes are complicated,and porous MPIs suffer poor mechanical properties.Also,increasing the fraction of free volume is a very effective way to lower D_(k)through introducing more ultra-low-D_(k)air component.However,most of this kind of MPIs lag far behind the fluorinated MPIs and the porous MPIs in terms of ultra-low D_(f),hindering the application of MPIs in high-speed communication devices.Thus,it is highly desirable to develop intrinsic ultra-low-D_(k)/D_(f)MPIs at high frequency with less fluoric groups and nonporous structure.Herein,we introduce a facile and effective strategy to lower D_(k)and D_(f)through introducing rigid and large sterically hindered aromatic groups into MPIs.On the one hand,their large steric hindrance effect leads to low D_(k)by increasing intrinsic free volume.On the other hand,the resulting highly stiff polymer chain and strong intermolecular interaction are favorable to reduce D_(f)by inhibiting dipole orientations.Based on this strategy,the spirobifluorene groups are preferred.The as-prepared MPIs show excellent dielectric performance with low D_(k)of 2.74–2.76 and low D_(f)of 0.00599 at 10 GHz,to some extent,exceeding the multiple fluorinated MPI with D_(k)/D_(f)of 2.67/0.00663 at 10 GHz.
基金Funded by the Fundamental Research Funds for the Central Universities (No. 2572021AW10)。
文摘In order to further study the reliability of macro evaluation indexes,molecular dynamics (MD) was applied to the evaluation of asphalt binder.Micro evaluation indexes (potential energy,surface free energy,solubility parameter and diffusion coefficient) of asphalt binder in different service phases (virgin,modified,aged and rejuvenated) were simulated.Combined with the variation characteristics of asphalt binder macro evaluation indexes (permeability,ductility,viscosity and softening point) in different service phases,the cross-scale correlation of macro-micro evaluation indexes was explored.The results show that the macro and micro evaluation indexes of asphalt binder have different characteristics in different service phases.The essence of the variation in the properties of asphalt binders is the difference in micro composition.In addition,there is a certain correlation between macro and micro evaluation indexes,which can be described by the gray relation theory.The cross-scale correlation of macro-micro evaluation indexes can provide a certain theoretical basis for the development of asphalt binder.
基金funded by the National Natural Science Foundation of China(Grant No.32101678)Henan Key Scientific Research Project(Grant Nos.22A210009,22B210010)+1 种基金Xinyang Agriculture and Forestry University Youth Fund Project(Grant No.QN2021021)Xinyang Agriculture and Forestry University High-Level Research Incubator Construction Project(Grant No.FCL202011).
文摘Soil salinity greatly impairs plant growth and crop productivity.Rice(Oryza sativa L.)is a salt-sensitive crop.To better understand the molecular mechanisms of salt tolerance in roots,the BGISEQ-500 sequencing platform was employed to elucidate transcriptome changes in rice roots after 0,3,24,and 72 h of salt stress.The results showed that root K+content decreased and Na+content increased rapidly after the initial stage of salt stress,but that fresh and dry weight in root did not significantly reduce.Compared to the control(no salt stress),1,292,453,and 486 differentially expressed genes(DEGs)were upregulated,respectively,and 939,894,and 646 DEGs were downregulated,respectively,after 3,24,and 72 h of salt treatment.The number of DEGs was higher during the early stage of salt stress(3 h)than in later stages(24 and 72 h).A number of DEGs involved in the response and adaptation to salt stress were related to protein kinase and calcium-binding,plant hormone signaling and metabolism,transcriptional regulation,metabolic pathways,antioxidant activity,and ion transport.Many of these DEGs were activated during the early stage of salt stress(3 h).The present study reports candidate salt-stressresponsive genes with the potential to genetically improve salt tolerance in rice elsewhere.
基金funded by the Key Research and Development Program of Jiangxi Province of China(No.20232BBF60026)。
文摘Fermented blueberry wine(BW)contains organic acids that may contribute to gastrointestinal discomfort,but the specific compounds responsible for diarrhea remain unclear.This study aimed to identify the key diarrhea-inducing organic acids by establishing a spectrum-effect relationship between chemical composition and biological activity.Ultra-high performance liquid chromatography(UHPLC)analysis revealed eight major organic acids in BW,with quinic acid predominating.Eight anion-exchange resin pretreatments combined with UHPLC-diode array detector successfully characterized the organic acid profiles of BW.Subsequent studies in mice demonstrated that resin treatment significantly alleviated BW-induced diarrhea in mice,reducing the diarrhea index and attenuating intestinal propulsion.Histopathological and biochemical analyses demonstrated restored intestinal integrity,improved gut barrier function,and normalized serum electrolytes,confirming that organic acids are the causative agents.Further spectrum-effect relationship was established through grey relational,orthogonal partial least squares,and Spearman rank correlation analysis,revealing quinic acid as exhibiting the strongest positive correlation with diarrhea incidence.The findings suggest that quinic acid is a primary contributor to diarrheal effects observed in BW.This study provides critical insights into regulating components in BW production to mitigate adverse gastrointestinal effects and a methodological foundation for material basis research in functional foods.
基金supported by Tehran University of Medical Science(Grant No.1400-03-148-55020)Royan Institute(401000177).
文摘Isolation of high-purity milk-derived extracellular vesicles(miEV)is complicated by protein impurities,such as casein.The focus of this study was to investigate whether the treatment of milk with acetic acid(AA),ethylenediaminetetraacetic acid(EDTA),and sodium citrate(SC),which are known chemicals to deplete caseins from miEV,alters their physicochemical and functional characteristics differently.For this purpose,following the treatment of milk with AA,EDTA,and SC,various subpopulations(12 K,35 K,70 K,and 100 K)of miEV were isolated by differential ultracentrifugation and characterized by protein quantification assay,gel electrophoresis,western blotting,dynamic light scattering,and scanning electron microscopy.In addition,the cellular uptake and immunomodulatory potential of miEV were also assessed.The results indicated that protein impurities,including caseins,were differentially depleted from miEV subpopulations by AA,EDTA,and SC.However,the acidification of milk induced partial protein aggregation.Additionally,miEV from both AA-and EDTA-treated milk exhibited a less smooth surface and higher uptake compared to those from SC-treated milk.Although all miEV significantly reduced T cell proliferation,a higher immunomodulatory potential was found in miEV from AA-treated milk.In conclusion,AA,SC,and EDTA have different efficiencies in improving the purity of miEV and can affect their physicochemical and functional characteristics differently.This issue may be critical for the efficient isolation of pure subpopulations of miEV for biomedical research.
基金funded by the the National Key Research and Development Program of China(2023YFD1700800)the National Natural Science Foundation of China(22276031,42107031)+2 种基金the Key Research Platform and Project for Higher Education Institutions in Guangdong Province in 2022(2022KCXTD049)China National Tobacco Corporation Guizhou Provincial Company Science and Technology Program(2022XM11)the Guangdong Foundation for Program of Science and Technology Research,China(2023B1212060044).
文摘Biochar is a promising technology for carbon storage and greenhouse gas(GHG)reduction,but optimizing it is challenging due to the complexity of natural systems.Machine learning(ML)and natural language processing(NLP)offer solutions through enhanced data analysis and pattern recognition,ushering in a new era of biochar research.
基金financial supports from National Natural Science Foundation of China(No.51801034,No.51771059)Guangdong Academy of Sciences Program(No.2020GDASYL-20200104030,No.2018GDASCX-0402,No.2017GDASCX-0202,No.2017GDASCX-0111)+2 种基金Guangdong Technical Research Program(No.2017B090916002,No.2017A070701027,No.2014B07075007)Guangdong Natural Science Foundation(No.2016A030312015,No.2017A030310315)Guangzhou Technical Research Program(No.201906010015,No.201707010455,No.201707010385).
文摘Plasma spray-physical vapor deposition(i.e.,PS-PVD)is a promising method for obtaining advanced environmental barrier coatings(EBCs).The EBCs must meet some requirements in the application,in which the thermal cycle performance affects the service lifetime.The preparation of artificial vertical cracks in Yb_(2)SiO_(5) coatings is an effective approach for meeting the requirements above because vertical cracks provide a strain tolerance.To clarify the formation mechanism of vertical cracks during the PSPVD,the effects of coating thickness and substrate temperature on the formation of vertical cracks were investigated.In addition,the interactions of spray powder and plasma flame during coating deposition were also characterized by optical spectroscopy.It is indicated that vertical cracks are formed due to a thermal expansion mismatch between Yb_(2)SiO_(5) and mullite coating,transient cooling after deposition and the nucleation of evaporated Yb_(2)SiO_(5) as well.
