For the quantitative determination of Fulvestrant, Benzyl alcohol, and Benzyl benzoate in Fulvestrant injection, an original RP-HPLC approach was developed. The gradient method was developed using HPLC and a Phenomene...For the quantitative determination of Fulvestrant, Benzyl alcohol, and Benzyl benzoate in Fulvestrant injection, an original RP-HPLC approach was developed. The gradient method was developed using HPLC and a Phenomenex Luna C8, 150 × 4.6 mm, i.d 3.0 μm particle size column with a gradient programme of mobile phases A and B. With a flow rate of 1.5 mL/minute, injection volume of 10 μL, and column temperature of 35°C, UV wavelength detection at 254 nm for Benzyl alcohol and Benzoyl Benzoate and 280 nm for Fulvestrant, mobile phase-A consists of DI water and mobile phase-B consists of Acetonitrile. The current study describes a single HPLC method for developing a Fulvestrant (Active), Benzyl alcohol (Cosolvent), and Benzyl Benzoate (Cosolvent) assay for Fulvestrant injection. The assay method was determined to be suitable for quantifying three components in the pharmaceutical product and was verified according to ICH guidelines.展开更多
The invasion of phytopathogens impacts the composition and associations of the internal microbial inhabitants.Leaf spot is one of the most devastating diseases in eggplant var.Mattu Gulla which is unique in terms of g...The invasion of phytopathogens impacts the composition and associations of the internal microbial inhabitants.Leaf spot is one of the most devastating diseases in eggplant var.Mattu Gulla which is unique in terms of geographic indication(GI)status.Leaf spot samples(asymptomatic and symptomatic)were collected to characterize the fungal community associated with them using culture-based and next-generation ITS rRNA-based metabarcoding approaches.Both methods showed that Ascomycota and Basidiomycota were the predominant phyla in both groups.In the asymptomatic group,Didymosphaeriaceae,Pleosporaceae,Trichomeriaceae,and Capnodiaceae were the most differentially abundant families.In contrast,Phaeosphaeriaceae,Pleosporaceae,Didymellaceae,Rhynchogastremataceae,and Bulleribasidiaceae were the most differentially abundant families in the symptomatic group.At the genus level,Cladosporium was the most differentially abundant genus in the asymptomatic group.In the symptomatic group Alternaria,Remotididymella,Vishniacozyma,Bulleribasidium,Occultifur,Epicoccum,and Loratospora were the abundant genera.The pathotroph-saprotrophic mode was the most common mode identified in both groups,with an increased abundance in the symptomatic group.Seven fungal families and two genera were identified as common according to the culture-based method and NGS analysis based on ITS rRNA metabarcoding.Our study indicated that the composition of the core microbial community varies with plant health status,and a combination of culturable and next-generation ITS rRNA-based metabarcoding approaches could be a reliable option for obtaining a detailed understanding of plant-associated fungal communities.展开更多
文摘For the quantitative determination of Fulvestrant, Benzyl alcohol, and Benzyl benzoate in Fulvestrant injection, an original RP-HPLC approach was developed. The gradient method was developed using HPLC and a Phenomenex Luna C8, 150 × 4.6 mm, i.d 3.0 μm particle size column with a gradient programme of mobile phases A and B. With a flow rate of 1.5 mL/minute, injection volume of 10 μL, and column temperature of 35°C, UV wavelength detection at 254 nm for Benzyl alcohol and Benzoyl Benzoate and 280 nm for Fulvestrant, mobile phase-A consists of DI water and mobile phase-B consists of Acetonitrile. The current study describes a single HPLC method for developing a Fulvestrant (Active), Benzyl alcohol (Cosolvent), and Benzyl Benzoate (Cosolvent) assay for Fulvestrant injection. The assay method was determined to be suitable for quantifying three components in the pharmaceutical product and was verified according to ICH guidelines.
基金the Manipal Academy of Higher Education(MAHE)and the Manipal School of Life Sciences,Manipal,Karnataka,India,and TIFAC-CORE and FIST,DST New Delhi,DBT-BUILDER grant(BT/INF/22/SP43065/2021)K-FIST,VGST,Govt.
文摘The invasion of phytopathogens impacts the composition and associations of the internal microbial inhabitants.Leaf spot is one of the most devastating diseases in eggplant var.Mattu Gulla which is unique in terms of geographic indication(GI)status.Leaf spot samples(asymptomatic and symptomatic)were collected to characterize the fungal community associated with them using culture-based and next-generation ITS rRNA-based metabarcoding approaches.Both methods showed that Ascomycota and Basidiomycota were the predominant phyla in both groups.In the asymptomatic group,Didymosphaeriaceae,Pleosporaceae,Trichomeriaceae,and Capnodiaceae were the most differentially abundant families.In contrast,Phaeosphaeriaceae,Pleosporaceae,Didymellaceae,Rhynchogastremataceae,and Bulleribasidiaceae were the most differentially abundant families in the symptomatic group.At the genus level,Cladosporium was the most differentially abundant genus in the asymptomatic group.In the symptomatic group Alternaria,Remotididymella,Vishniacozyma,Bulleribasidium,Occultifur,Epicoccum,and Loratospora were the abundant genera.The pathotroph-saprotrophic mode was the most common mode identified in both groups,with an increased abundance in the symptomatic group.Seven fungal families and two genera were identified as common according to the culture-based method and NGS analysis based on ITS rRNA metabarcoding.Our study indicated that the composition of the core microbial community varies with plant health status,and a combination of culturable and next-generation ITS rRNA-based metabarcoding approaches could be a reliable option for obtaining a detailed understanding of plant-associated fungal communities.
