Alzheimer’s disease is a progressive brain disorder and complex mechanisms are involved in the physiopathology of Alzheimer’s disease.However,there is data suggesting that inflammation plays a role in its developmen...Alzheimer’s disease is a progressive brain disorder and complex mechanisms are involved in the physiopathology of Alzheimer’s disease.However,there is data suggesting that inflammation plays a role in its development and progression.Indeed,some non-steroidal antiinflammatory drugs,such as meloxicam,which act by inhibiting cyclooxygenase-2 have been used as neuroprotective agents in different neurodegenerative disease models.The purpose of this study was to investigate the effects of co-nanoencapsulated curcumin and meloxicam in lipid core nanocapsules(LCN)on cognitive impairment induced by amyloid-beta peptide injection in mice.LCN were prepared by the nanoprecipitation method.Male Swiss mice received a single intracerebroventricular injection of amyloid-beta peptide aggregates(fragment 25–35,3 nmol/3μL)or vehicle and were subsequently treated with curcumin-loaded LCN(10 mg/kg)or meloxicam-loaded LCN(5 mg/kg)or meloxicam+curcumin-co-loaded LCN(5 and 10 mg/kg,respectively).Treatments were given on alternate days for 12 days(i.e.,six doses,once every 48 hours,by intragastric gavage).Our data showed that amyloid-beta peptide infusion caused long-term memory deficits in the inhibitory avoidance and object recognition tests in mice.In the inhibitory avoidance test,both meloxicam and curcumin formulations(oil or co-loaded LCN)improved amyloid-beta-induced memory impairment in mice.However,only meloxicam and curcumin-co-loaded LCN attenuated non-aversive memory impairment in the object recognition test.Moreover,the beneficial effects of meloxicam and curcuminco-loaded LCN could be explained by the anti-inflammatory properties of these drugs through cortical cyclooxygenase-2 downregulation.Our study suggests that the neuroprotective potential of meloxicam and curcumin co-nanoencapsulation is associated with cortical cyclooxygenase-2 modulation.This study was approved by the Committee on Care and Use of Experimental Animal Resources,the Federal University of Pampa,Brazil(approval No.02-2015)on April 16,2015.展开更多
Flaxseed (Linum usitatissimum L.) is composed mainly of bioactive components such as polyphenols, polyunsaturated fatty acids, fiber and lignans. Flaxseed can be found in different presentation forms (grain or flour) ...Flaxseed (Linum usitatissimum L.) is composed mainly of bioactive components such as polyphenols, polyunsaturated fatty acids, fiber and lignans. Flaxseed can be found in different presentation forms (grain or flour) and varieties (brown or golden);however, questions have arisen as to whether the presentation form and/or variety may influence the health effects. The objective of this study was to evaluate the effects on blood pressure, anthropometric and oxidative parameters in healthy human volunteers. All subjects received 40 gram aliquots of flaxseed and were instructed to consume them in their entirety mixed with water in the morning for a period of 14 days. Oxidative parameters showed significant reductions (p < 0.05) in oxidative damage to lipids and proteins via dietary intervention with golden flaxseed grains. There were no significant differences in anthropometric parameters, blood pressure, DNA damage and micronuclei frequency after 14-day supplementation. This research indicates that golden flaxseed grains can be a valuable adjunct for disease prevention and protecting the organism against oxidative damage.展开更多
Cancer remains one of the deadliest diseases globally.While various treatments exist,their side effects often reduce patients’quality of life,and some cancer types resist current therapies.Promising to fill in the po...Cancer remains one of the deadliest diseases globally.While various treatments exist,their side effects often reduce patients’quality of life,and some cancer types resist current therapies.Promising to fill in the possible lack of traditional therapies,evidence from literature has shown that phenolic acids are great candidates for use as adjuvant therapies against cancer.This study used bibliometric analysis to explore the anticancer potential of phenolic acids.Over the past 20 years,research in this field has increased significantly,with approximately 75%of publications being original research.Keywords like“cytotoxicity”,“antioxidant”,“antioxidant activity”,“cancer”,“anticancer activity”,“apoptosis”,“inflammation”,“antiproliferative activity”,“phytochemistry”,“polyphenols”,“cell proliferation”,“oxidative stress”,“caffeic acid”,“gallic acid”,“ferulic acid”,“quercetin”,“NF-κβ”,and“TNF-α”were frequently used.China led in the number of publications and funding.Among the phenolic acids studied,caffeic acid,gallic acid,and ferulic acid showed the most promising anticancer potential.Key mechanisms linked to their effects include apoptosis,oxidative stress,and inflammatory responses.The journals publishing these studies had an average impact factor of 5.75 and were categorized as Q1/Q2 in the journal citation reports(JCR).Thus,for the first time,we showed through this bibliometric study several aspects of phenolic acids and their anticancer potential in the last 20 years.The results provide comprehension that phenolic acids have gained space in the science and possibly represent new horizons in adjuvant therapies against cancer.展开更多
The use of Stevia rebaudiana stems leaves can negatively interfere with the process of obtaining and purifying sweeteners.If separated,they account for 30%of the plant’s biomass,besides being used as a supplement to ...The use of Stevia rebaudiana stems leaves can negatively interfere with the process of obtaining and purifying sweeteners.If separated,they account for 30%of the plant’s biomass,besides being used as a supplement to animal feed.This pioneering study performed commonly used extractions from the leaves.We characterized the extracts(methanolic and hydroalcoholic)and their fractions(ethyl acetate)by UHPLC-MS/MS.Besides,we evaluated their antioxidant,antidiabetic,and antiobesity capacity in vitro.The extracts had 17%of phenolics,with antioxidant activity of about 65%.The fraction with 26%of phenolics showed an antioxidant capacity of 89%.The lipase inhibition rate was higher than the fraction,about 2.5 times.The same happened with the enzymesα-amylase andα-glucosidase,where the hydroalcoholic extract showed 30 and 90%inhibition,respectively.These results show that the stem is a valuable co-product for the Stevia production chain.展开更多
The valorization of winery pomace,traditionally considered waste,offers a sustainable approach to harnessing its bioactive compounds.In this study,we investigated the antiproliferative potential of winery pomace extra...The valorization of winery pomace,traditionally considered waste,offers a sustainable approach to harnessing its bioactive compounds.In this study,we investigated the antiproliferative potential of winery pomace extract against thyroid cancer cells.An ultrasound-assisted extraction technique was employed to extract the bioactive compounds.The optimal conditions for this process were determined through a Rotational Central Composite Design(50℃,4 g/L solid-to-liquid ratio,40%ethanol concentration,and 79 W ultrasound power).The resulting extract demonstrated robust antioxidant activity,reflected in the elevated total phenolic content(TPC)and free radical scavenging ability.Furthermore,the effect of the extract on peripheral blood mononuclear cells(PBMCs)and TPC-1 cancer cells was assessed.PBMCs exposed to the extract at 10 mg/mL concentration exhibited enhanced viability,whereas cancer cells displayed concentration-dependent cytotoxic responses,indicating a selective anticancer effect.These findings underscore the potential of winery pomace extract as a natural antioxidant with promising anticancer properties,warranting further investigation for potential applications in cancer therapy.Moreover,to the best of the authors’knowledge,this work represents one of the first publications to demonstrate the potential of wine pomace extract to inhibit thyroid cancer cells.展开更多
基金This study was supported by the Rio Grande do Sul Science Foundation(FAPERGS),grants#16/2551-0000207-0 and 16/0526-5(PRONUPEQ)(to SP)National Counsel of Technological and Scientific Development(CNPq)(Universal grants#423435/2016-7 and 460122/2014-2)and for student scholarships(to CL,SEH and DSÁ).
文摘Alzheimer’s disease is a progressive brain disorder and complex mechanisms are involved in the physiopathology of Alzheimer’s disease.However,there is data suggesting that inflammation plays a role in its development and progression.Indeed,some non-steroidal antiinflammatory drugs,such as meloxicam,which act by inhibiting cyclooxygenase-2 have been used as neuroprotective agents in different neurodegenerative disease models.The purpose of this study was to investigate the effects of co-nanoencapsulated curcumin and meloxicam in lipid core nanocapsules(LCN)on cognitive impairment induced by amyloid-beta peptide injection in mice.LCN were prepared by the nanoprecipitation method.Male Swiss mice received a single intracerebroventricular injection of amyloid-beta peptide aggregates(fragment 25–35,3 nmol/3μL)or vehicle and were subsequently treated with curcumin-loaded LCN(10 mg/kg)or meloxicam-loaded LCN(5 mg/kg)or meloxicam+curcumin-co-loaded LCN(5 and 10 mg/kg,respectively).Treatments were given on alternate days for 12 days(i.e.,six doses,once every 48 hours,by intragastric gavage).Our data showed that amyloid-beta peptide infusion caused long-term memory deficits in the inhibitory avoidance and object recognition tests in mice.In the inhibitory avoidance test,both meloxicam and curcumin formulations(oil or co-loaded LCN)improved amyloid-beta-induced memory impairment in mice.However,only meloxicam and curcumin-co-loaded LCN attenuated non-aversive memory impairment in the object recognition test.Moreover,the beneficial effects of meloxicam and curcuminco-loaded LCN could be explained by the anti-inflammatory properties of these drugs through cortical cyclooxygenase-2 downregulation.Our study suggests that the neuroprotective potential of meloxicam and curcumin co-nanoencapsulation is associated with cortical cyclooxygenase-2 modulation.This study was approved by the Committee on Care and Use of Experimental Animal Resources,the Federal University of Pampa,Brazil(approval No.02-2015)on April 16,2015.
文摘Flaxseed (Linum usitatissimum L.) is composed mainly of bioactive components such as polyphenols, polyunsaturated fatty acids, fiber and lignans. Flaxseed can be found in different presentation forms (grain or flour) and varieties (brown or golden);however, questions have arisen as to whether the presentation form and/or variety may influence the health effects. The objective of this study was to evaluate the effects on blood pressure, anthropometric and oxidative parameters in healthy human volunteers. All subjects received 40 gram aliquots of flaxseed and were instructed to consume them in their entirety mixed with water in the morning for a period of 14 days. Oxidative parameters showed significant reductions (p < 0.05) in oxidative damage to lipids and proteins via dietary intervention with golden flaxseed grains. There were no significant differences in anthropometric parameters, blood pressure, DNA damage and micronuclei frequency after 14-day supplementation. This research indicates that golden flaxseed grains can be a valuable adjunct for disease prevention and protecting the organism against oxidative damage.
基金funded by grants from Fundação de Amparo a Pesquisa e Inovação do Estado de Santa Catarina(FAPESC)(APK,Grant:2023TR564).APK and MDB are 2-CNPq Research Fellow.
文摘Cancer remains one of the deadliest diseases globally.While various treatments exist,their side effects often reduce patients’quality of life,and some cancer types resist current therapies.Promising to fill in the possible lack of traditional therapies,evidence from literature has shown that phenolic acids are great candidates for use as adjuvant therapies against cancer.This study used bibliometric analysis to explore the anticancer potential of phenolic acids.Over the past 20 years,research in this field has increased significantly,with approximately 75%of publications being original research.Keywords like“cytotoxicity”,“antioxidant”,“antioxidant activity”,“cancer”,“anticancer activity”,“apoptosis”,“inflammation”,“antiproliferative activity”,“phytochemistry”,“polyphenols”,“cell proliferation”,“oxidative stress”,“caffeic acid”,“gallic acid”,“ferulic acid”,“quercetin”,“NF-κβ”,and“TNF-α”were frequently used.China led in the number of publications and funding.Among the phenolic acids studied,caffeic acid,gallic acid,and ferulic acid showed the most promising anticancer potential.Key mechanisms linked to their effects include apoptosis,oxidative stress,and inflammatory responses.The journals publishing these studies had an average impact factor of 5.75 and were categorized as Q1/Q2 in the journal citation reports(JCR).Thus,for the first time,we showed through this bibliometric study several aspects of phenolic acids and their anticancer potential in the last 20 years.The results provide comprehension that phenolic acids have gained space in the science and possibly represent new horizons in adjuvant therapies against cancer.
基金supported by the Arauc´aria Foundation Agreement(020/21)Postgraduate Program in Biochemistry(PBQ),Dean of Research and Postgraduate Studies(PPG)and a scholarship provided by the Coordination for the Improvement of Higher Education Personnel(CAPES).
文摘The use of Stevia rebaudiana stems leaves can negatively interfere with the process of obtaining and purifying sweeteners.If separated,they account for 30%of the plant’s biomass,besides being used as a supplement to animal feed.This pioneering study performed commonly used extractions from the leaves.We characterized the extracts(methanolic and hydroalcoholic)and their fractions(ethyl acetate)by UHPLC-MS/MS.Besides,we evaluated their antioxidant,antidiabetic,and antiobesity capacity in vitro.The extracts had 17%of phenolics,with antioxidant activity of about 65%.The fraction with 26%of phenolics showed an antioxidant capacity of 89%.The lipase inhibition rate was higher than the fraction,about 2.5 times.The same happened with the enzymesα-amylase andα-glucosidase,where the hydroalcoholic extract showed 30 and 90%inhibition,respectively.These results show that the stem is a valuable co-product for the Stevia production chain.
文摘The valorization of winery pomace,traditionally considered waste,offers a sustainable approach to harnessing its bioactive compounds.In this study,we investigated the antiproliferative potential of winery pomace extract against thyroid cancer cells.An ultrasound-assisted extraction technique was employed to extract the bioactive compounds.The optimal conditions for this process were determined through a Rotational Central Composite Design(50℃,4 g/L solid-to-liquid ratio,40%ethanol concentration,and 79 W ultrasound power).The resulting extract demonstrated robust antioxidant activity,reflected in the elevated total phenolic content(TPC)and free radical scavenging ability.Furthermore,the effect of the extract on peripheral blood mononuclear cells(PBMCs)and TPC-1 cancer cells was assessed.PBMCs exposed to the extract at 10 mg/mL concentration exhibited enhanced viability,whereas cancer cells displayed concentration-dependent cytotoxic responses,indicating a selective anticancer effect.These findings underscore the potential of winery pomace extract as a natural antioxidant with promising anticancer properties,warranting further investigation for potential applications in cancer therapy.Moreover,to the best of the authors’knowledge,this work represents one of the first publications to demonstrate the potential of wine pomace extract to inhibit thyroid cancer cells.
