AIM:To study the anti-neoplastic effect of Haimiding and its mechanisms of action.METHODS:Experiments using MIT and colony formation were carried out to study the in vitro anti-neoplastic action of Haimiding, its in v...AIM:To study the anti-neoplastic effect of Haimiding and its mechanisms of action.METHODS:Experiments using MIT and colony formation were carried out to study the in vitro anti-neoplastic action of Haimiding, its in vivo anti-neoplastic action was studied by observing its effect on the weight of tumors in FC mice and S180, H22 tumor bearing mice, as well as their life spans.The effect of Haimiding on cell apoptosis and different stages of cell cycles in human gastric carcinoma cells were studied by flow cytometry. Its effect on [Ca^2+]i of human gastric carcinoma cells and the source of Ca^2+ during the change of [Ca^2+]i were observed by confocal laser scanning technique.RESULTS:Haimiding showed a definite cytotoxicity to 8 human tumor cell lines, which was most prominent against BGC-823, Eca-109 and HCT-8 tumor cells. It also exhibited an obvious inhibition on colony formation of the above tumor cell lines, which was most prominent in Eca-109 tumor cells. It showed obvious inhibition on the growth of tumor in FC mice and S180 bearing mice as well as prolonged the life span of H22 bearing mice. It was able to induce apoptosis and elevate intracellular [Ca^2+]i concentration of tumor cells.The source of Ca^2+ came from both extracellular Ca^2+ influx and intracellular Ca^2+ release.CONCLUSION:Haimiding is composed of a TCM preparation and 5-flurouracil.Its anti-neoplastic potency is highly enhanced by synergism as compared with either one of its components. Its mechanisms of anti-neoplastic action can be attributed to its action to initiate apoptosis of tumor cells by opening the membrane calcium channel and inducing intracellular Ca^2+ release to elevate [Ca^2+]i of the tumor cells.展开更多
AIM: To study the effect of Haimiding on the functioning of red cell membrane of FC and H22 tumor-bearing mice. METHODS: The membrane fluidity of red cells is measured with DPH fluorescence probe as a marker; the amou...AIM: To study the effect of Haimiding on the functioning of red cell membrane of FC and H22 tumor-bearing mice. METHODS: The membrane fluidity of red cells is measured with DPH fluorescence probe as a marker; the amount of red cell membrane proteins is measured using polyacrylamide gel electrophoresis; the amount of sialic acid (SA) on the surface of red cell membrane and the scalability of these cells are measured using colorimetric analysis. RESULTS: Haimiding can lower the membrane fluidity of red cells in tumor-bearing mice and the amount of their membrane proteins, while increasing the amount of sialic acid in the membrane of red cells in these mice and enhancing the ability of the membrane of their red cells to reseal. CONCLUSION: The anti-tumor effect of Haimiding on tumor-bearing mice is due to its ability to improve and restore the functions of the membrane of their red cell and to enhance the immune effect of the organisms.展开更多
基金Supported by the National Natural Science Foundation of China,No.30271598Outstanding Post Doctoral Research Foundation of China,No.17(1999)+1 种基金Heiiongjiang Province Foundation for Talented Youth No.J 9906Natural Science Foundation of Heilongjiang Provi
文摘AIM:To study the anti-neoplastic effect of Haimiding and its mechanisms of action.METHODS:Experiments using MIT and colony formation were carried out to study the in vitro anti-neoplastic action of Haimiding, its in vivo anti-neoplastic action was studied by observing its effect on the weight of tumors in FC mice and S180, H22 tumor bearing mice, as well as their life spans.The effect of Haimiding on cell apoptosis and different stages of cell cycles in human gastric carcinoma cells were studied by flow cytometry. Its effect on [Ca^2+]i of human gastric carcinoma cells and the source of Ca^2+ during the change of [Ca^2+]i were observed by confocal laser scanning technique.RESULTS:Haimiding showed a definite cytotoxicity to 8 human tumor cell lines, which was most prominent against BGC-823, Eca-109 and HCT-8 tumor cells. It also exhibited an obvious inhibition on colony formation of the above tumor cell lines, which was most prominent in Eca-109 tumor cells. It showed obvious inhibition on the growth of tumor in FC mice and S180 bearing mice as well as prolonged the life span of H22 bearing mice. It was able to induce apoptosis and elevate intracellular [Ca^2+]i concentration of tumor cells.The source of Ca^2+ came from both extracellular Ca^2+ influx and intracellular Ca^2+ release.CONCLUSION:Haimiding is composed of a TCM preparation and 5-flurouracil.Its anti-neoplastic potency is highly enhanced by synergism as compared with either one of its components. Its mechanisms of anti-neoplastic action can be attributed to its action to initiate apoptosis of tumor cells by opening the membrane calcium channel and inducing intracellular Ca^2+ release to elevate [Ca^2+]i of the tumor cells.
基金Supported by National Science Foundation of China (No. 30271598,30300284)Heilongjiang Science Foundation (No. ZJY03-04)Ministry of Information Industry (No. 01XK230002)Heilongjiang Department of Education (Major Project No. 9551z006)Innovative Fund for Distinguished University and College Teachers of Heilongjiang Province, No. 2001015
文摘AIM: To study the effect of Haimiding on the functioning of red cell membrane of FC and H22 tumor-bearing mice. METHODS: The membrane fluidity of red cells is measured with DPH fluorescence probe as a marker; the amount of red cell membrane proteins is measured using polyacrylamide gel electrophoresis; the amount of sialic acid (SA) on the surface of red cell membrane and the scalability of these cells are measured using colorimetric analysis. RESULTS: Haimiding can lower the membrane fluidity of red cells in tumor-bearing mice and the amount of their membrane proteins, while increasing the amount of sialic acid in the membrane of red cells in these mice and enhancing the ability of the membrane of their red cells to reseal. CONCLUSION: The anti-tumor effect of Haimiding on tumor-bearing mice is due to its ability to improve and restore the functions of the membrane of their red cell and to enhance the immune effect of the organisms.
