Studies in recent years have shown that aquatic pollution by microplastics(MPs)can be considered to pose additional stress to amphibian populations.However,our knowledge of how MPs affect amphibians is very rudimentar...Studies in recent years have shown that aquatic pollution by microplastics(MPs)can be considered to pose additional stress to amphibian populations.However,our knowledge of how MPs affect amphibians is very rudimentary,and even more limited is our understanding of their effects in combination with other emerging pollutants.Thus,we aimed to evaluate the possible toxicity of polyethylene MPs(PE-MPs)(alone or in combination with a mix of pollutants)on the health of Physalaemus cuvieri tadpoles.After 30 days of exposure,multiple biomarkers were measured,including morphological,biometric,and developmental indices,behavioral parameters,mutagenicity,cytotoxicity,antioxidant and cholinesterase responses,as well as the uptake and accumulation of PE-MPs in animals.Based on the results,there was no significant change in any of the parameters measured in tadpoles exposed to treatments,but induced stress was observed in tadpoles exposed to PE-MPs combined with themixture of pollutants,reflecting significant changes in physiological and biochemical responses.Through principal component analysis(PCA)and integrated biomarker response(IBR)assessment,effects induced by pollutants in each test group were distinguished,confirming that the exposure of P.cuvieri tadpoles to the PE-MPs in combination with a mix of emerging pollutants induces an enhanced stress response,although the uptake and accumulation of PE-MPs in these animalswas reduced.Thus,our study provides newinsight into the danger to amphibians of MPs coexisting with other pollutants in aquatic environments.展开更多
Recent evidence suggests that Brazilian Cerrado has been largely converted, mainly due to agribusiness activities and urbanization. Thus, the present work aimed to obtain the diagnosis of a watershed characterized by ...Recent evidence suggests that Brazilian Cerrado has been largely converted, mainly due to agribusiness activities and urbanization. Thus, the present work aimed to obtain the diagnosis of a watershed characterized by the expansion of sugar cane fields in the Brazilian Cerrado (Southeastern), employing integrity landscape indicators (Hemeroby, Urbanity Index, Landscape Vulnerability Index, and Water Quality Index) to verify the landscape naturalness, and the consequences of these actions to the ecosystem services provision. Remote sensing data for the years 1998, 2008, and 2018 were employed to verify the land-use changes in the Feijão River watershed (southeastern Brazil), as well as a matrix that considers the land use typologies and their capability to provide ecosystem services. The study area is classified as mixed land use, with the main categories related to agribusiness (sugar cane, pasture, bare soil). The Hemeroby and Urban Index denote naturalness declines over the years, and the observed patterns negatively impact ecosystem service provision. Landscape Vulnerability Index and Water Quality Index demonstrated that remaining vegetation cannot effectively retain pollutants, and severe impairments in the resilience of natural components of key ecosystem service providers (i.e. water and native vegetation) were observed. The results allowed the identification of priority areas for recovery and the maintenance of ecosystem services in agricultural lands.展开更多
基金supporting the financing of this study(Process#307743/2018–7)the granting of research productivity grants to Dr.Malafaia G.(Proc.#307743/2018–7)and Dr.Melo e Silva D.(Process#307652/2018-1)+1 种基金for the scientific initiation scholarships to students Silva A.M.and Luz T.M.In addition,the authors thank the Federal Institute of Goiano(GO,Brazil)granting important financial assistance for the publication of this study(Proc.#23219.000139.2022-17).
文摘Studies in recent years have shown that aquatic pollution by microplastics(MPs)can be considered to pose additional stress to amphibian populations.However,our knowledge of how MPs affect amphibians is very rudimentary,and even more limited is our understanding of their effects in combination with other emerging pollutants.Thus,we aimed to evaluate the possible toxicity of polyethylene MPs(PE-MPs)(alone or in combination with a mix of pollutants)on the health of Physalaemus cuvieri tadpoles.After 30 days of exposure,multiple biomarkers were measured,including morphological,biometric,and developmental indices,behavioral parameters,mutagenicity,cytotoxicity,antioxidant and cholinesterase responses,as well as the uptake and accumulation of PE-MPs in animals.Based on the results,there was no significant change in any of the parameters measured in tadpoles exposed to treatments,but induced stress was observed in tadpoles exposed to PE-MPs combined with themixture of pollutants,reflecting significant changes in physiological and biochemical responses.Through principal component analysis(PCA)and integrated biomarker response(IBR)assessment,effects induced by pollutants in each test group were distinguished,confirming that the exposure of P.cuvieri tadpoles to the PE-MPs in combination with a mix of emerging pollutants induces an enhanced stress response,although the uptake and accumulation of PE-MPs in these animalswas reduced.Thus,our study provides newinsight into the danger to amphibians of MPs coexisting with other pollutants in aquatic environments.
文摘Recent evidence suggests that Brazilian Cerrado has been largely converted, mainly due to agribusiness activities and urbanization. Thus, the present work aimed to obtain the diagnosis of a watershed characterized by the expansion of sugar cane fields in the Brazilian Cerrado (Southeastern), employing integrity landscape indicators (Hemeroby, Urbanity Index, Landscape Vulnerability Index, and Water Quality Index) to verify the landscape naturalness, and the consequences of these actions to the ecosystem services provision. Remote sensing data for the years 1998, 2008, and 2018 were employed to verify the land-use changes in the Feijão River watershed (southeastern Brazil), as well as a matrix that considers the land use typologies and their capability to provide ecosystem services. The study area is classified as mixed land use, with the main categories related to agribusiness (sugar cane, pasture, bare soil). The Hemeroby and Urban Index denote naturalness declines over the years, and the observed patterns negatively impact ecosystem service provision. Landscape Vulnerability Index and Water Quality Index demonstrated that remaining vegetation cannot effectively retain pollutants, and severe impairments in the resilience of natural components of key ecosystem service providers (i.e. water and native vegetation) were observed. The results allowed the identification of priority areas for recovery and the maintenance of ecosystem services in agricultural lands.
