The increased incidence of invasive and opportunistic mycoses is probably related to the growth of the immunocompromised population, such as people living with HIV. This study is a literature review that aims to analy...The increased incidence of invasive and opportunistic mycoses is probably related to the growth of the immunocompromised population, such as people living with HIV. This study is a literature review that aims to analyze the frequency of invasive fungal infections in people living with HIV. In most studies evaluated, <em>Pneumocystis</em> pneumonia was the most frequent invasive fungal infection among people living with HIV, and cryptococcosis was the second most frequent. Invasive fungal infections are associated with greater morbidity and mortality in people living with HIV. The most important highlighted information is that the lack of epidemiological data on fungal infections in the studied populations was reported by most studies. Therefore, there is a need for further studies to assess the frequency of invasive fungal infection in people living with HIV, which may serve as subsidies for the implementation of strategies for the prevention and management, with a consequent increase in the quality of life and reduction of morbidity/mortality in this population.展开更多
Background:Edible vaccines produced in genetically modified plants represent a novel approach to immunization,to express antigens capable of eliciting immune responses at mucosal surfaces.This method provides a potent...Background:Edible vaccines produced in genetically modified plants represent a novel approach to immunization,to express antigens capable of eliciting immune responses at mucosal surfaces.This method provides a potential cost-effective solution for vaccine delivery,particularly beneficial in resource-limited settings.Aim:This review aims to assess the efficacy of edible vaccines derived from transgenic plants in eliciting immune responses in animal models against various infectious and parasitic diseases.Methods:A comprehensive search of PubMed,Embase,and Scopus up to June 2024 identified 138 references.Following Cochrane guidelines,PRISMA standards,and the CAMARADES risk of bias checklist,16 studies met the inclusion criteria and focused on edible vaccines for animal immunization.Results:The findings indicate that edible vaccines can effectively elicit both humoral and mucosal immune responses in animals.For instance,transgenic plants expressing antigens for hepatitis B and human papillomavirus demonstrated high efficacy,with significant increases in antigen-specific antibody levels post-immunization.Studies on bacterial diseases using LT-B antigens from Escherichia coli expressed in corn showed robust,sustained antibody responses.Additionally,for parasitic diseases,transgenic rice expressing Ascaris antigen reduced larval burden,indicating protective efficacy.However,variations in immune response depending on plant type and antigen stability highlight the need for further optimization to achieve consistent efficacy across different diseases.展开更多
Multifunctional scaffolds with host defense peptides designed for regenerative endodontics are desirable nanobiotechnological tools for dentistry.Here,different scaffolds were tested for use during the pulp revascular...Multifunctional scaffolds with host defense peptides designed for regenerative endodontics are desirable nanobiotechnological tools for dentistry.Here,different scaffolds were tested for use during the pulp revascularization process,including poly(vinyl alcohol)-PVA hydrogels or resins,collagen hydrogels and poly(vinyl alcohol)PVA/Chitosan(PVA/CS)nanofibers.Based on time to degradation(21 days),nanofibers were chosen to be incorporated with ciprofloxacin and IDR-1002(each at 50 mg/g).Nanofibers containing ciprofloxacin and IDR-1002 had anti-biofilm activity against Enterococcus faecalis,Staphylococcus aureus and a multispecies oral biofilm,besides anti-inflammatory activities.The in vivo subcutaneous tissue response to tooth fragments filled with nanofibers demonstrated a pulp-like tissue formation,when compared to empty teeth fragments.Thus,we designed a strong antimicrobial,immunomodulatory and regenerative candidate for pulp revascularization and regeneration procedures.展开更多
文摘The increased incidence of invasive and opportunistic mycoses is probably related to the growth of the immunocompromised population, such as people living with HIV. This study is a literature review that aims to analyze the frequency of invasive fungal infections in people living with HIV. In most studies evaluated, <em>Pneumocystis</em> pneumonia was the most frequent invasive fungal infection among people living with HIV, and cryptococcosis was the second most frequent. Invasive fungal infections are associated with greater morbidity and mortality in people living with HIV. The most important highlighted information is that the lack of epidemiological data on fungal infections in the studied populations was reported by most studies. Therefore, there is a need for further studies to assess the frequency of invasive fungal infection in people living with HIV, which may serve as subsidies for the implementation of strategies for the prevention and management, with a consequent increase in the quality of life and reduction of morbidity/mortality in this population.
文摘Background:Edible vaccines produced in genetically modified plants represent a novel approach to immunization,to express antigens capable of eliciting immune responses at mucosal surfaces.This method provides a potential cost-effective solution for vaccine delivery,particularly beneficial in resource-limited settings.Aim:This review aims to assess the efficacy of edible vaccines derived from transgenic plants in eliciting immune responses in animal models against various infectious and parasitic diseases.Methods:A comprehensive search of PubMed,Embase,and Scopus up to June 2024 identified 138 references.Following Cochrane guidelines,PRISMA standards,and the CAMARADES risk of bias checklist,16 studies met the inclusion criteria and focused on edible vaccines for animal immunization.Results:The findings indicate that edible vaccines can effectively elicit both humoral and mucosal immune responses in animals.For instance,transgenic plants expressing antigens for hepatitis B and human papillomavirus demonstrated high efficacy,with significant increases in antigen-specific antibody levels post-immunization.Studies on bacterial diseases using LT-B antigens from Escherichia coli expressed in corn showed robust,sustained antibody responses.Additionally,for parasitic diseases,transgenic rice expressing Ascaris antigen reduced larval burden,indicating protective efficacy.However,variations in immune response depending on plant type and antigen stability highlight the need for further optimization to achieve consistent efficacy across different diseases.
基金supported by Conselho Nacional de Desenvolvimento Científico e Tecnologico(CNPq)(409196/2018-5),Coordenaçao de Aperfeiçoamento de Pessoal de Nível Superior(CAPES)(88887.202222/2018-00)Fundaçao de Apoioa Pesquisa do Distrito Federal(FAPDF)(00193-00000782/2021-63),and Fundaçao de Apoio ao Desenvolvimento do Ensino,Ciˆencia e Tecnologia do Estado de Mato Grosso do Sul(FUNDECT)(59/300.397/2015+2 种基金022/2018028973)In addition,this study was supported by a Canadian Institutes of Health Research Foundation grant FDN-154287 to R.E.W.Hancock.HE is the recipient of a UBC Killam Fellowship and a Research Trainee Award from the Michael Smith Foundation for Health Research(MSFHR).R.E.W.Hancock is a Canada Research Chair in Health and Genomics and a UBC Killam Professor.We also acknowledge Professor Marcelo Oliveira Rodrigues and Chemistry Institute(University of Brasilia)for all support.
文摘Multifunctional scaffolds with host defense peptides designed for regenerative endodontics are desirable nanobiotechnological tools for dentistry.Here,different scaffolds were tested for use during the pulp revascularization process,including poly(vinyl alcohol)-PVA hydrogels or resins,collagen hydrogels and poly(vinyl alcohol)PVA/Chitosan(PVA/CS)nanofibers.Based on time to degradation(21 days),nanofibers were chosen to be incorporated with ciprofloxacin and IDR-1002(each at 50 mg/g).Nanofibers containing ciprofloxacin and IDR-1002 had anti-biofilm activity against Enterococcus faecalis,Staphylococcus aureus and a multispecies oral biofilm,besides anti-inflammatory activities.The in vivo subcutaneous tissue response to tooth fragments filled with nanofibers demonstrated a pulp-like tissue formation,when compared to empty teeth fragments.Thus,we designed a strong antimicrobial,immunomodulatory and regenerative candidate for pulp revascularization and regeneration procedures.
