OBJECTIVE To investigate the neuroprotective effect and possible mechanisms of lute.olin-7-O-β-D-glucuronide(LGU) against focalcerebral ischemic injury.METHODS The focal cerebral ischemic injury model was established...OBJECTIVE To investigate the neuroprotective effect and possible mechanisms of lute.olin-7-O-β-D-glucuronide(LGU) against focalcerebral ischemic injury.METHODS The focal cerebral ischemic injury model was established by middle cerebral artery occlusion(MCAO).Male Sprague Dawley rats were randomly divided into sham group,model group(MCAO),LGU group(0.24,0.72 and2.16 mg·kg^(-1)) and positive control group(Edaravone at 5 mg·kg^(-1)).LGU was injected intravenously 30 min after MCAO.Neurological severity score,infarct volume and brain water content were detected 24 h after MCAO and the levels of Na+-K+ ATPase,Ca2 + ATPase,TNF-α and IL-1β were detected to explore the possible mechanisms.For the therapeutic time window test,LGU(0.72 mg·kg^(-1)) was injected intrave.nously 0.5,2,4,6,8,10 and 12 h respectively after MCAO.To evaluate motion behavior,LGU were injected intravenously 30 min after MCAO and once per day during detection period.The changes of motor coordination were detected by rotating rod method and grip strength analysis,and the changes of gaits were detected using DigiGait Imaging System.RESULTS LGU improved the neurological severity score,infarct volume ratio and brain water content.The therapeutic time window of LGU for cerebral infarction and brain edema was at least 6 h and for neurological dysfunction was 12 h.LGU also prolonged the latency on rotarod,increased the forelimb tension and improved 8 gait parameters,including stance duration,stride length,stance width,paw area,paw area variability,gait symmetry,ataxia coefficient and tau propulsion.Furthermore,LGU increased Na^+-K^+-ATPase and Ca^(2+)-ATPase levels in the cortex and hippocampus in the ischemic side,reduced the levels of TNF-α and IL-1β in the serum.CONCLUSION LGU has a significant neuroprotective effect against cerebral ischemic injury via improving energy metabolism and reducing inflammation.展开更多
OBJECTIVE To investigate the protective effect and mechanisms of luteolin-7-O-β-dglucuronide(LGU) on oxygen glucose deprivation(OGD)-induced H9C2 cardiomyocytes injury.METH.ODS The protective effect of LGU on OGD-ind...OBJECTIVE To investigate the protective effect and mechanisms of luteolin-7-O-β-dglucuronide(LGU) on oxygen glucose deprivation(OGD)-induced H9C2 cardiomyocytes injury.METH.ODS The protective effect of LGU on OGD-induced H9C2 cardiomyocytes death were investigated by MTT assay.The microfilament change of H9C2 cardiomyocytes was detected by phalloidin staining and the lactate dehydrogenase(LDH) leakage rate was also detected by LDH kit.In order to explore the possible mechanisms of LGU,ATP content,intracellular Ca^(2+) fluorescent intensity and concentra.tion,mitochondrial membrane potential(MMP)and the expressions of apoptosis-related proteins were detected by ATP kit,CLSM(Fluo-3/AM probe),Ca^(2+) kit,CLSM(JC-1 probe) and western blotting meth.od,respectively.RESULTS The inhibition of H9C2 cardiomyocyte survival rate inducedby OGD was improvedby pretreated with LGU in a concentrationdependent manner.The microfilaments injury as well as the increase of LDH leakage rate were also improvedby pretreated with LGU.The ATP content was significantly decreased,intracellular Ca^(2+) fluorescent intensity and concentration were significantly increased and the MMP was significantly decreased 4 hafter OGD.LGU significantly reversed the de.crease of intracellular ATP content,the increase of Ca^(2+) fluorescent intensity and concentration and the decrease of MMP.The release of cytochrome C,the expressionsof caspase-9 and caspase-3 in H9C2 cardiomyocytes were increased 16 h after OGD.LGUsignificantly inhibited the changes of these apop.tosis-related proteins.CONCLUSION LGU has a significant protective effect against OGD-induced H9C2 cardiomyocytes injury through inhibiting calcium overload,increasing ATP content,improving mi.tochondrial function and inhibiting apoptosis.展开更多
Cancer is a leading cause of death worldwide. Cancer treatments by chemotherapeutic agents, surgery, and radiation have not been highly effective in reducing the incidence of cancers and increasing the survival rate o...Cancer is a leading cause of death worldwide. Cancer treatments by chemotherapeutic agents, surgery, and radiation have not been highly effective in reducing the incidence of cancers and increasing the survival rate of cancer patients. In recent years, plant-derived compounds have attracted considerable attention as alternative cancer remedies for enhancing cancer prevention and treatment because of their low toxicities, low costs, and low side effects. Ellagic acid(EA) is a natural phenolic constituent. Recent in vitro and in vivo experiments have revealed that EA elicits anticarcinogenic effects by inhibiting tumor cell proliferation, inducing apoptosis, breaking DNA binding to carcinogens, blocking virus infection, and disturbing inflammation, angiogenesis, and drug-resistance processes required for tumor growth and metastasis. This review enumerates the anticarcinogenic actions and mechanisms of EA. It also discusses future directions on the applications of EA.展开更多
Sulphur dioxide is used in the vinification process as an antimicrobial agent, but it is also considered as an important risk factor for liver diseases. Stilbenes have inhibitory effects on the growth of lactic acid b...Sulphur dioxide is used in the vinification process as an antimicrobial agent, but it is also considered as an important risk factor for liver diseases. Stilbenes have inhibitory effects on the growth of lactic acid bacteria strains isolated from wine. The authors investigated the viability of obtaining free-SO2 resveratrol-enriched red wines. Substitution of sulfite （7 g/100kg） for resveratrol （150 mg/L and 300 mg/L） in the vinification process did not change the basic physical and chemical properties of wine or its sensorial profile To the best of the authors＇ knowledge this is the first time that this type of vinification procedure is reported.展开更多
Wine is the product of the alcoholic fermentation of the grape (Vitis vinifera). As such, it is a hydroalcoholic solution with a variable content of nutritional and functional (polyphenols) molecules, the latter invol...Wine is the product of the alcoholic fermentation of the grape (Vitis vinifera). As such, it is a hydroalcoholic solution with a variable content of nutritional and functional (polyphenols) molecules, the latter involved in its antioxidant potential. The organoleptic variables of wine, together with its potential positive effects on health of a light-moderate intake, have always been topics of great interest within the cultures. In the label of wine, alcohol is the only declared variable. On the other hand, there is no information about the content of “positive” molecules, such as those associated with the antioxidant power. This value could be very important to classify the wines, helping oenologist and nutritionist in qualifying them as a component of Mediterranean diet. Moreover, one of the most critical aspects in evaluating the role of wine in human health through epidemiological prospective studies is the quality of the products used and their antioxidant potential. This research aimed to optimize and validate an analytical approach based on a portable device (SCIO?), using NIRS (near-infrared reflectance spectroscopy). It allows the measure of TAP (total antioxidant power) of wine through the glass. Research findings are promising. To the best of our knowledge, this is the first time that an easy-to-use and cheap hand-held scanner is validated to measure the TAP of a beverage.展开更多
Objective To develop a new antimicrobial sensitivity test model for oral products in vitro.Methods A biofilm artificial mouth model for antimicrobial sensitivity tests was established by modifying the LKB chromatograp...Objective To develop a new antimicrobial sensitivity test model for oral products in vitro.Methods A biofilm artificial mouth model for antimicrobial sensitivity tests was established by modifying the LKB chromatography chamber. Using sodium fluoride and Tea polyphenol as antimicrobial agent and Streptococcus mu-tans as target, sensitivity tests were studied. Results The modeling biofilm assay resulted in a MIC of 1. 28mg/ ml for fluoride against S. mutans, which was 32 times the MIC for broth maco-dilution method. The differential resistance of bacteria bioflim to antimicrobial agent relative to planktonic cells was also demonstrated. Conclusion The biofilm artificial mouth model may be useful in oral products test.展开更多
基金supported by Young and Middle-aged Teacher Career Development Support Plan of Shenyang Pharmaceutical University(ZQN2016002) and Science and Technology Funds from Department of Education of Liaoning province(2016101633L3)
文摘OBJECTIVE To investigate the neuroprotective effect and possible mechanisms of lute.olin-7-O-β-D-glucuronide(LGU) against focalcerebral ischemic injury.METHODS The focal cerebral ischemic injury model was established by middle cerebral artery occlusion(MCAO).Male Sprague Dawley rats were randomly divided into sham group,model group(MCAO),LGU group(0.24,0.72 and2.16 mg·kg^(-1)) and positive control group(Edaravone at 5 mg·kg^(-1)).LGU was injected intravenously 30 min after MCAO.Neurological severity score,infarct volume and brain water content were detected 24 h after MCAO and the levels of Na+-K+ ATPase,Ca2 + ATPase,TNF-α and IL-1β were detected to explore the possible mechanisms.For the therapeutic time window test,LGU(0.72 mg·kg^(-1)) was injected intrave.nously 0.5,2,4,6,8,10 and 12 h respectively after MCAO.To evaluate motion behavior,LGU were injected intravenously 30 min after MCAO and once per day during detection period.The changes of motor coordination were detected by rotating rod method and grip strength analysis,and the changes of gaits were detected using DigiGait Imaging System.RESULTS LGU improved the neurological severity score,infarct volume ratio and brain water content.The therapeutic time window of LGU for cerebral infarction and brain edema was at least 6 h and for neurological dysfunction was 12 h.LGU also prolonged the latency on rotarod,increased the forelimb tension and improved 8 gait parameters,including stance duration,stride length,stance width,paw area,paw area variability,gait symmetry,ataxia coefficient and tau propulsion.Furthermore,LGU increased Na^+-K^+-ATPase and Ca^(2+)-ATPase levels in the cortex and hippocampus in the ischemic side,reduced the levels of TNF-α and IL-1β in the serum.CONCLUSION LGU has a significant neuroprotective effect against cerebral ischemic injury via improving energy metabolism and reducing inflammation.
基金supported by Young and Middle-aged Teacher Career Development Support Plan of Shenyang Pharmaceutical University(ZQN2016002) Science and Technology Funds from Department of Education of Liaoning Province(2016101633L3)
文摘OBJECTIVE To investigate the protective effect and mechanisms of luteolin-7-O-β-dglucuronide(LGU) on oxygen glucose deprivation(OGD)-induced H9C2 cardiomyocytes injury.METH.ODS The protective effect of LGU on OGD-induced H9C2 cardiomyocytes death were investigated by MTT assay.The microfilament change of H9C2 cardiomyocytes was detected by phalloidin staining and the lactate dehydrogenase(LDH) leakage rate was also detected by LDH kit.In order to explore the possible mechanisms of LGU,ATP content,intracellular Ca^(2+) fluorescent intensity and concentra.tion,mitochondrial membrane potential(MMP)and the expressions of apoptosis-related proteins were detected by ATP kit,CLSM(Fluo-3/AM probe),Ca^(2+) kit,CLSM(JC-1 probe) and western blotting meth.od,respectively.RESULTS The inhibition of H9C2 cardiomyocyte survival rate inducedby OGD was improvedby pretreated with LGU in a concentrationdependent manner.The microfilaments injury as well as the increase of LDH leakage rate were also improvedby pretreated with LGU.The ATP content was significantly decreased,intracellular Ca^(2+) fluorescent intensity and concentration were significantly increased and the MMP was significantly decreased 4 hafter OGD.LGU significantly reversed the de.crease of intracellular ATP content,the increase of Ca^(2+) fluorescent intensity and concentration and the decrease of MMP.The release of cytochrome C,the expressionsof caspase-9 and caspase-3 in H9C2 cardiomyocytes were increased 16 h after OGD.LGUsignificantly inhibited the changes of these apop.tosis-related proteins.CONCLUSION LGU has a significant protective effect against OGD-induced H9C2 cardiomyocytes injury through inhibiting calcium overload,increasing ATP content,improving mi.tochondrial function and inhibiting apoptosis.
基金supported in part by grants from The National Science and Technology Foundation for Rural Development in China during the "12th Five-Year-Plan" (Grant No. 2011BAD33B02)The National Natural Science Foundation of China (Grant No. 81300323)
文摘Cancer is a leading cause of death worldwide. Cancer treatments by chemotherapeutic agents, surgery, and radiation have not been highly effective in reducing the incidence of cancers and increasing the survival rate of cancer patients. In recent years, plant-derived compounds have attracted considerable attention as alternative cancer remedies for enhancing cancer prevention and treatment because of their low toxicities, low costs, and low side effects. Ellagic acid(EA) is a natural phenolic constituent. Recent in vitro and in vivo experiments have revealed that EA elicits anticarcinogenic effects by inhibiting tumor cell proliferation, inducing apoptosis, breaking DNA binding to carcinogens, blocking virus infection, and disturbing inflammation, angiogenesis, and drug-resistance processes required for tumor growth and metastasis. This review enumerates the anticarcinogenic actions and mechanisms of EA. It also discusses future directions on the applications of EA.
文摘Sulphur dioxide is used in the vinification process as an antimicrobial agent, but it is also considered as an important risk factor for liver diseases. Stilbenes have inhibitory effects on the growth of lactic acid bacteria strains isolated from wine. The authors investigated the viability of obtaining free-SO2 resveratrol-enriched red wines. Substitution of sulfite （7 g/100kg） for resveratrol （150 mg/L and 300 mg/L） in the vinification process did not change the basic physical and chemical properties of wine or its sensorial profile To the best of the authors＇ knowledge this is the first time that this type of vinification procedure is reported.
文摘Wine is the product of the alcoholic fermentation of the grape (Vitis vinifera). As such, it is a hydroalcoholic solution with a variable content of nutritional and functional (polyphenols) molecules, the latter involved in its antioxidant potential. The organoleptic variables of wine, together with its potential positive effects on health of a light-moderate intake, have always been topics of great interest within the cultures. In the label of wine, alcohol is the only declared variable. On the other hand, there is no information about the content of “positive” molecules, such as those associated with the antioxidant power. This value could be very important to classify the wines, helping oenologist and nutritionist in qualifying them as a component of Mediterranean diet. Moreover, one of the most critical aspects in evaluating the role of wine in human health through epidemiological prospective studies is the quality of the products used and their antioxidant potential. This research aimed to optimize and validate an analytical approach based on a portable device (SCIO?), using NIRS (near-infrared reflectance spectroscopy). It allows the measure of TAP (total antioxidant power) of wine through the glass. Research findings are promising. To the best of our knowledge, this is the first time that an easy-to-use and cheap hand-held scanner is validated to measure the TAP of a beverage.
文摘Objective To develop a new antimicrobial sensitivity test model for oral products in vitro.Methods A biofilm artificial mouth model for antimicrobial sensitivity tests was established by modifying the LKB chromatography chamber. Using sodium fluoride and Tea polyphenol as antimicrobial agent and Streptococcus mu-tans as target, sensitivity tests were studied. Results The modeling biofilm assay resulted in a MIC of 1. 28mg/ ml for fluoride against S. mutans, which was 32 times the MIC for broth maco-dilution method. The differential resistance of bacteria bioflim to antimicrobial agent relative to planktonic cells was also demonstrated. Conclusion The biofilm artificial mouth model may be useful in oral products test.
