The effect of native medical plant “Clammy Inula” (Inula viscosa L.) in combination with a low dose of the fungicide iprodione (Rovral®) against gray mould disease (Botryis cinerea Pers.) was evaluated in vit...The effect of native medical plant “Clammy Inula” (Inula viscosa L.) in combination with a low dose of the fungicide iprodione (Rovral®) against gray mould disease (Botryis cinerea Pers.) was evaluated in vitro and in vivo. The results revealed that the plant extract had antifungal effect on mycelial growth rate and conidial germiation of pathogen isolates. The plant extract at the concentration of 1% - 4% and the fungicide iprodione at the concentration of 0.2 - 9 μg·ml-1 significantly reduced the mycelium growth and germination. In addition, the plant extract at the concentration of 2% - 4% and iprodione at the concentration of 300 - 600 μg·ml-1 significantly reduced the disease severity (%) on bean plants compared to the controls. The reduction of mycelium growth, germination, and disease severity was positively correlated with increasing plant extract and fungicide concentrations. The combination of a low dose of plant extract and fungicide at (EC50) was able to reduce disease severity of gray mold by 84%. While, plant extract and fungicide alone reduced disease severity by 46% & 39% and by 70% & 76% for the isolate (Bo5-10 and Bc 99), respectively, compared with controls. The application of water extracts of I. viscose in combination of a low dose of an effective fungicide (iprodione) can be a feasible tool in reducing gray mould disease severity, but farther studies are still needed under field conditions to evaluate efficacy against the disease under field conditions.展开更多
The effects of biofungicide formula containing the biocontrol agent Trichoderma harzianum (Jn14) as wettable powder in combination with the fungicides pyrimethanil and a mixture of cyprodinil and flydioxonil on Botryt...The effects of biofungicide formula containing the biocontrol agent Trichoderma harzianum (Jn14) as wettable powder in combination with the fungicides pyrimethanil and a mixture of cyprodinil and flydioxonil on Botrytis cinerea of strawberry in vitro, in vivo and in greenhouse were studied. The pathogen B. cinerea was more affected in vitro by low concentrations of the fungicides tested above 0.4 μg·ml-1 than the bioagent T. harzianum (Jn14). The later was almost insensitive to pyrimethanil. In the same direction, gray mould disease severity on strawberry detached leaves and whole plants was reduced up to 89% by the tested fungicides, compared to the control, while the application of T. harzianum (Jn14) alone reduced disease severity up to 45% on strawberry detached leaves, compared to the control. In the integrated control approach, the combination of T. harzianum (Jn14) with higher concentrations of the tested fungicides (ED50) completely inhibited strawberry gray mould disease severity in pots and the greenhouse.展开更多
[Objective]The paper was to explore application of nativo in control against cucumber powdery mildew.[Method]Taking ＂Zhenghuang 3＂ cucumber as the material,the effects of nativo on growth of cucumber at different pe...[Objective]The paper was to explore application of nativo in control against cucumber powdery mildew.[Method]Taking ＂Zhenghuang 3＂ cucumber as the material,the effects of nativo on growth of cucumber at different periods were studied by soaking seeds and spraying treatment on stems and leaves.The field efficacy trial against powdery mildew was also carried out to comprehensively evaluate control effect of nativo against powdery mildew.[Result]Seed soaking by nativo had good preventive effect on powdery mildew,while plant height,root length and increase rate of stem diameter were significantly lower than that of hexaconazole.Rates of plant height inhibition were 15.62% and 4.65% at seedling and flowering stage after spraying 60mg/L of nativo,respectively, which were significantly lower than that of hexaconazole.The control efficacies of nativo were 73.82%-84.26% in field trial,which was as good as that of hexaconazole but higher than that of Topsin-M.[Conclusion]Nativo is safe to growth of cucumber,with good control effect on powdery mildew.It can be used as an alternative fungicide to control cucumber powdery.展开更多
文摘The effect of native medical plant “Clammy Inula” (Inula viscosa L.) in combination with a low dose of the fungicide iprodione (Rovral®) against gray mould disease (Botryis cinerea Pers.) was evaluated in vitro and in vivo. The results revealed that the plant extract had antifungal effect on mycelial growth rate and conidial germiation of pathogen isolates. The plant extract at the concentration of 1% - 4% and the fungicide iprodione at the concentration of 0.2 - 9 μg·ml-1 significantly reduced the mycelium growth and germination. In addition, the plant extract at the concentration of 2% - 4% and iprodione at the concentration of 300 - 600 μg·ml-1 significantly reduced the disease severity (%) on bean plants compared to the controls. The reduction of mycelium growth, germination, and disease severity was positively correlated with increasing plant extract and fungicide concentrations. The combination of a low dose of plant extract and fungicide at (EC50) was able to reduce disease severity of gray mold by 84%. While, plant extract and fungicide alone reduced disease severity by 46% & 39% and by 70% & 76% for the isolate (Bo5-10 and Bc 99), respectively, compared with controls. The application of water extracts of I. viscose in combination of a low dose of an effective fungicide (iprodione) can be a feasible tool in reducing gray mould disease severity, but farther studies are still needed under field conditions to evaluate efficacy against the disease under field conditions.
文摘The effects of biofungicide formula containing the biocontrol agent Trichoderma harzianum (Jn14) as wettable powder in combination with the fungicides pyrimethanil and a mixture of cyprodinil and flydioxonil on Botrytis cinerea of strawberry in vitro, in vivo and in greenhouse were studied. The pathogen B. cinerea was more affected in vitro by low concentrations of the fungicides tested above 0.4 μg·ml-1 than the bioagent T. harzianum (Jn14). The later was almost insensitive to pyrimethanil. In the same direction, gray mould disease severity on strawberry detached leaves and whole plants was reduced up to 89% by the tested fungicides, compared to the control, while the application of T. harzianum (Jn14) alone reduced disease severity up to 45% on strawberry detached leaves, compared to the control. In the integrated control approach, the combination of T. harzianum (Jn14) with higher concentrations of the tested fungicides (ED50) completely inhibited strawberry gray mould disease severity in pots and the greenhouse.
基金Supported by Scientific and Technological Projects of Henan Province(112102110060)
文摘[Objective]The paper was to explore application of nativo in control against cucumber powdery mildew.[Method]Taking ＂Zhenghuang 3＂ cucumber as the material,the effects of nativo on growth of cucumber at different periods were studied by soaking seeds and spraying treatment on stems and leaves.The field efficacy trial against powdery mildew was also carried out to comprehensively evaluate control effect of nativo against powdery mildew.[Result]Seed soaking by nativo had good preventive effect on powdery mildew,while plant height,root length and increase rate of stem diameter were significantly lower than that of hexaconazole.Rates of plant height inhibition were 15.62% and 4.65% at seedling and flowering stage after spraying 60mg/L of nativo,respectively, which were significantly lower than that of hexaconazole.The control efficacies of nativo were 73.82%-84.26% in field trial,which was as good as that of hexaconazole but higher than that of Topsin-M.[Conclusion]Nativo is safe to growth of cucumber,with good control effect on powdery mildew.It can be used as an alternative fungicide to control cucumber powdery.
