Plants deploy a two-layered immune system:pathogen-associated molecular pattern(PAMP)-triggered immunity(PTl)and effector-triggered immunity(ETI).While PTI is initiated by cell surface receptors,ETI relies on intracel...Plants deploy a two-layered immune system:pathogen-associated molecular pattern(PAMP)-triggered immunity(PTl)and effector-triggered immunity(ETI).While PTI is initiated by cell surface receptors,ETI relies on intracellular NLR receptors that recognize pathogen effectors(Jones et al.,2024).The nucleoporin CONSTITUTIVE EXPRESSER OF PATHOGENESIS-RELATED GENES 5(CPR5)is a key negative regulator of ETI.CPR5 integrates nuclear transport,cell cycle control,and RNA processing to suppress immune signaling(Wang et al.,2014;Gu et al.,2016;Peng et al.,2022).Recent work revealed that CPR5 also modulates immunity through another nucleoporin,GUANYLATE-BINDING PROTEIN-LIKE 3(GBPL3),which interaCtS with PWWP-DOMAIN INTERACTOR OF POLYCOMBS1(PWO1),a key component of the chromatin-associated methyltransferase POLYCOMB REPRESSIVE COMPLEX 2(PRC2)(Reimann et al.,2023;Pan et al.,2025).These findings suggest unexplored roles for chromatin remodeling in the CPR5-mediated immunity.展开更多
A new flatid genus in the tribe Selizini(Hemiptera:Fulgoromorpha:Flatidae)from southwestern China is proposed:Wattlea Peng,Ai&Zhang gen.nov.based on W.distincta Ai,Peng&Zhang sp.nov.and W.wandingensis Peng,Ai&...A new flatid genus in the tribe Selizini(Hemiptera:Fulgoromorpha:Flatidae)from southwestern China is proposed:Wattlea Peng,Ai&Zhang gen.nov.based on W.distincta Ai,Peng&Zhang sp.nov.and W.wandingensis Peng,Ai&Zhang sp.nov.from Yunnan,China.A third new species in a different genus,Zecheuna curva Ai,Peng&Zhang sp.nov.from Hainan,China,is described.Diagnoses,detailed descriptions,and illustrations are provided for each taxon,as well as comparisons with closely-related genera.展开更多
Glutathione S-transferases (GSTs) represent a large and diverse enzyme family ubiquitously distributed across the plant kingdom. These proteins catalyze the conjugation of glutathione (GSH) with electrophilic substrat...Glutathione S-transferases (GSTs) represent a large and diverse enzyme family ubiquitously distributed across the plant kingdom. These proteins catalyze the conjugation of glutathione (GSH) with electrophilic substrates in response to various stress conditions. Beyond their role in stress adaptation, certain GSTs are integral regulators of plant growth and development, contributing to a range of physiological processes. Most GST proteins exhibit dual enzymatic activities, functioning as both transferases and peroxidases, which enables their involvement in diverse cellular processes, including detoxification and stress responses. Recent advancements, particularly in X-ray crystallography, have enabled detailed structural analysis of GST proteins, significantly enhancing our understanding of their biological functions. This review offers a comprehensive overview of the classification and structural characteristics of GSTs in plants. It also highlights recent findings on their roles in plant growth and development, cell signaling, catalytic transport, and stress tolerance. Furthermore, key scientific challenges related to GSTs are discussed, focusing on their potential applications in agriculture. These insights aim to facilitate the screening of functional GST genes and support molecular breeding efforts across diverse crop species.展开更多
Licochalcone A(LCA)is a characteristic compound in licorice Glycyrrhiza inflata and is widely utilized in pharmaceutical and cosmetic industries.However,the biosynthetic pathway and regulatory mechanisms of LCA remain...Licochalcone A(LCA)is a characteristic compound in licorice Glycyrrhiza inflata and is widely utilized in pharmaceutical and cosmetic industries.However,the biosynthetic pathway and regulatory mechanisms of LCA remain poorly understood.In this study,we first found the accumulation of LCA is induced by methyl jasmonate(MeJA).Given that MYB transcriptional factors are well-documented as key regulators of flavonoid biosynthesis,we identified a total of 147 GiR2R3-MYB genes in G.inflata,which were classified into 28 subgroups.The chromosome distributions,sequence characteristics,gene structures,duplication events and cis-acting elements were also investigated.Through integrated analysis of GiR2R3-MYBs expression patterns across different tissues and under MeJA treatment,along with phylogenetic relationship,we identified GiMYB76—a MeJA-inducible MYB transcription factor—as a potential regulator of LCA accumulation.Functional validation showed that transgenic hairy roots overexpressing GiMYB76 exhibited a significant increase in LCA content.DAP-seq analysis of GiMYB76 revealed potential target genes involved in flavonoid biosynthesis regulation.Subsequent promoter activity assay verified that GiMYB76 can bind to the promoter and activate the expression of GiCHS4.Consistently,overexpression of GiCHS4 in G.inflata hairy roots also significantly enhanced LCA production.This study not only clarifies that GiMYB76 transcriptionally activated GiCHS4 to promote LCA biosynthesis but also provides valuable insights for basic research on licorice and the development of related industries.展开更多
Ammonium toxicity in plants remains poorly understood despite extensive research.While nitrate is known to benefit plant growth,the synergistic effects of nitrate in mitigating ammonium toxicity,even at low concentrat...Ammonium toxicity in plants remains poorly understood despite extensive research.While nitrate is known to benefit plant growth,the synergistic effects of nitrate in mitigating ammonium toxicity,even at low concentrations,are not fully elucidated.This review delves into the physiological and molecular nature of this phenomenon.To date,nitrate-dependent alleviation of ammonium toxicity is the result of cumulative consequences of the role of nitrate as a nutrient and signal in plant performance.The ability to counteract the ammonium-induced acidification through nitrate uptake and metabolism,the enhancement of potassium uptake as an essential nitrate counterion,and the nitratedependent signaling of key factors involved in ammonium assimilation,ROS scavenging,and growth hormone biosynthesis,are the most relevant hallmarks.In addition,evidence suggests that the availability of nitrate and ammonium has driven ecological selection in plants,determining current N preferences,and may have led to the selection of nitrate-dependent and ammonium-sensitive domesticated crops and the inefficient use of N fertilizers in agriculture.As ammonium toxicity limits N fertilization options and reduces agricultural yields,when it could be a more sustainable and cheaper alternative to nitrate,this review provides a better understanding of how plants use nitrate to counteract the problematic aspects of ammonium nutrition.展开更多
The Loxostege sticticalis(Lepidoptera:Pyralidae)is a major migratory pest of agriculture and animal husbandry in Asia and Europe.Utilizing plant volatile organic compounds(pVOCs)as attractants for monitoring and contr...The Loxostege sticticalis(Lepidoptera:Pyralidae)is a major migratory pest of agriculture and animal husbandry in Asia and Europe.Utilizing plant volatile organic compounds(pVOCs)as attractants for monitoring and controlling pests is considered an environmentally friendly and effective method.However,limited knowledge exists regarding applying pVOCs to manage L.sticticalis.Here,volatile compounds released by Chenopodium album,Setaria viridis,and Medicago sativa,the three preferred oviposition plants for L.sticticalis females,were collected using dynamic headspace sampling techniques.A total of 55 distinct compounds were identified through gas chromatography-mass spectrometry(GC-MS),and 16 compounds in the concentration range from 0.001 to 100µgµL–1 elicited consistently enhanced electrophysiological responses in both male and female L.sticticalis.Subsequently,the attraction potential of four bioactive compounds-linalool,cis-anethole,trans-2-hexenal,and 1-octen-3-ol-were further confirmed by indoor behavioral bioassays.The blends of linalool,cis-anethole,trans-2-hexenal,and 1-octen-3-ol mixed at ratios of 5:1:5:10(formulation No.25)and 5:1:1:10(formulation No.21)were highly attractive to L.sticticalis adults.Field-trapping assays indicated that lure No.2 baited with formulation 21 demonstrated superior efficacy in field trapping.These findings suggest that pVOC-based attractants can be effectively employed for monitoring and mass trapping L.sticticalis adults,providing insights into the development of botanical attractants.展开更多
Tea plant(Camellia sinensis(L.)O.Kuntze)is a cold-sensitive leaf-harvesting crop whose growth,yield,and processed tea quality are all inhibited by low temperatures.Therefore,identifying the regulatory genes involved i...Tea plant(Camellia sinensis(L.)O.Kuntze)is a cold-sensitive leaf-harvesting crop whose growth,yield,and processed tea quality are all inhibited by low temperatures.Therefore,identifying the regulatory genes involved in tea plant growth and freezing tolerance is crucial for genetic improvement.WRKY transcription factors regulate various plant processes,including growth and development,stress responses,and metabolite biosynthesis.However,the molecular network through which WRKY coordinates these pathways in tea plants remains unclear.In this study,we revealed that CsWRKY57L,a cold-inducible WRKY IIc subfamily member,positively regulated freezing tolerance by directly promoting flavonoid accumulation in tea plants.Transient suppression of CsWRKY57L weakened the freezing tolerance of tea plants by reducing flavonoid content and suppressing the C-repeat-binding factor(CBF)-cold-responsive(COR)gene pathway.In contrast,heterologous overexpression of CsWRKY57L in Arabidopsis had the opposite effect.Additionally,overexpression of CsWRKY57L inhibited reproductive development and accelerated senescence in Arabidopsis.Interaction analysis revealed that CsWRKY57L directly binds to the promoters of CsSWEET1a,CsSWEET15,and AtSWEET15,which encode sugar transporters essential for plant reproductive development,and inhibits their transcription.Overall,the study revealed a dual role of CsWRKY57L in promoting freezing tolerance via flavonoid biosynthesis and inhibiting reproductive development by regulating SWEETs expression.This study uncovers a novel mechanism whereby CsWRKY57L coordinately regulates both stress responses and growth in tea plants,providing a molecular basis for breeding low-temperature-tolerant varieties with restricted reproductive development.展开更多
Ascorbate(Asc),commonly known as vitamin C,is a vital molecule for plant growth,development,and stress resilience.It is also known to play a crucial role in various physiological processes,including photosynthesis,cel...Ascorbate(Asc),commonly known as vitamin C,is a vital molecule for plant growth,development,and stress resilience.It is also known to play a crucial role in various physiological processes,including photosynthesis,cell division,and differentiation.This article thoroughly explores the processes governing the metabolism of Asc in plants and its roles in physiological functions.It lays down a robust theoretical groundwork for delving into Asc production,transportation,functions,and its potential applications in stress alleviation and horticulture.Furthermore,recent studies indicate that Asc plays a role in regulating fruit development and affecting postharvest storage characteristics,thereby influencing fruit ripening and resilience to stress.Hence,there is a growing importance in studying the synthesis and utilization of Asc in plants.Although the critical role of Asc in controlling plant redox signals has been extensively studied,the precise mechanisms by which it manages cellular redox homeostasis to maintain the equilibrium between reactive oxygen scavenging and cell redox signaling remain elusive.This gap in knowledge presents fresh opportunities to explore how the production of Asc in plants is regulated and how plants react to environmental stressors.Furthermore,this article delves into the potential for a comprehensive investigation into the essential function of Asc in fruits,the development of Asc-rich fruits,and the enhancement of postharvest storage properties.展开更多
Heat stress reduces theanine content in tea plants,but the underlying molecular mechanism remains unclear.In this study,a temperature gradient treatment(20℃,25℃,30℃,and 35℃)was performed to unveil the effect of he...Heat stress reduces theanine content in tea plants,but the underlying molecular mechanism remains unclear.In this study,a temperature gradient treatment(20℃,25℃,30℃,and 35℃)was performed to unveil the effect of heat stress on biosynthesis and accumulation of theanine.We found that heat stress induced metabolic changes,characterized by decreased theanine content and increased catechin levels.In addition,heat stress up-regulated the expression of the class B heat shock transcription factor gene CsHSFB2c,while significantly suppressing the transcription of key theanine biosynthetic genes CsTS1 and CsGS1.Functional studies showed that silencing CsHSFB2c increased theanine content,while its overexpression significantly decreased theanine levels.Consistent with these changes,silencing CsHSFB2c upregulated the expression of CsTS1 and CsGS1,while overexpression of CsHSFB2c downregulated their expression.Yeast one-hybrid(Y1H)and dual-luciferase reporter gene(Dual-LUC)assays showed that CsHSFB2c directly binds to the promoters of CsTS1 and CsGS1 and inhibits their expression.These results demonstrate that CsHSFB2c mediates heat-induced suppression of theanine biosynthesis by directly inhibiting the expression of CsTS1 and CsGS1.This study provides a theoretical basis for improving the heat resistance and quality of tea plants via molecular breeding.展开更多
Plant tissue culture represents an advanced biotechnological technique for propagating and conserving threatened plant species efficiently.This method enables the rapid production of genetically identical plants under...Plant tissue culture represents an advanced biotechnological technique for propagating and conserving threatened plant species efficiently.This method enables the rapid production of genetically identical plants under controlled sterile laboratory conditions(in vitro).Its applications span forestry,horticulture,and,crucially,plant breeding.Nanoparticles have emerged as innovative tools to address limitations in conventional plant tissue culture,offering diverse functionalities based on their unique physicochemical properties.This review focuses on the utilization of nanotechnology in enhancing various aspects of plant tissue culture.Nanoparticles,such as silver and zinc oxide,have demonstrated significant roles as antimicrobial agents and anti-browning agents.They also serve as elicitors,stimulating callus proliferation,root elongation,rapid shoot formation,and the enhanced production of bioactive compounds on a large scale.Furthermore,nanoparticles contribute to mitigating oxidative stress within cells,thereby promoting increased callus formation,elongated roots,and elevated production of secondary metabolites.Their influence extends to somaclonal variation and genetic transformation processes within plant tissue culture.These contributions collectively underscore the potential of nanoparticles to foster more efficient,sustainable,and scalable biotechnological solutions in in vitro culture.The implications extend to reducing resource dependency and mitigating environmental impacts,positioning nanotechnology as a transformative approach in sustainable plant biotechnology.展开更多
[Objective]To systematically isolate and purify the polysaccharide from the mycelium of Streptomyces rochei D74(SRP),elucidate its fine structure,and evaluate the effect of the purified polysaccharide fraction on the ...[Objective]To systematically isolate and purify the polysaccharide from the mycelium of Streptomyces rochei D74(SRP),elucidate its fine structure,and evaluate the effect of the purified polysaccharide fraction on the growth of Salvia miltiorrhiza hairy roots and the biosynthesis of tanshinones,along with the underlying mechanism.[Methods]The crude polysaccharide was extracted using hot water,which was followed by ethanol precipitation and deproteinization via the Sevag method.Further purification was performed using DEAE-52 anionexchange chromatography and Sephadex G-100 gel filtration chromatography.The physicochemical properties and structural features of the main active fraction,SRP-W-2,were systematically characterized by Fourier transform infrared spectroscopy(FTIR),high performance liquid chromatography-mass spectrometry(HPLC-MS),and nuclear magnetic resonance(NMR).The effects of SRP-W-2 on hairy root growth and the biosynthesis of tanshinones were assessed by measuring biomass,tanshinone content,and the expression levels of key biosynthetic genes.[Results]SRP-W-2 was obtained with a yield of 2.41%.It was primarily composed of glucose and galactose at a molar ratio of 12.53:1.Structural analysis revealed that the backbone of SRP-W-2 consisted of→4)-α-D-Glcp-(1→and→4)-α-D-Galp-(1→residues,with branching points at→4,6)-α-D-Glcp-(1→and→4,6)-α-D-Galp-(1→.The side chain was identified asα-D-Glcp-(1→4)-α-DGlcp-(1→.Bioactivity assays demonstrated that SRP-W-2 significantly enhanced both the biomass of S.miltiorrhiza hairy roots and the accumulation of tanshinones.After 15 d of treatment with 50 mg/L SRP-W-2,the dry weight of the hairy roots increased by 37.52%.Meanwhile,the content of cryptotanshinone(CT),dihydrotanshinone I(DT-I),tanshinone I(T-I),and tanshinone IIA(TIIA)was increased by 19.0-fold,6.4-fold,2.8-fold,and 4.8-fold,respectively.Gene expression analysis further indicated that SRP-W-2 up-regulated key genes involved in the tanshinone biosynthetic pathway,including HMGR,DXS,DXR,and GGPPS.[Conclusion]The polysaccharide fraction SRP-W-2 from S.rochei D74 simultaneously promoted the growth of S.miltiorrhiza hairy roots and the biosynthesis of tanshinones,demonstrating its potential as an effective elicitor.This study provided a new strategy for the utilization and development of S.miltiorrhiza resources.展开更多
Maintaining optimal crop nutritional levels is crucial for maximizing yield and enhancing stress resistance.In addition to the 17 essential nutrients,there are many plant-beneficial elements:silicon,aluminum,selenium,...Maintaining optimal crop nutritional levels is crucial for maximizing yield and enhancing stress resistance.In addition to the 17 essential nutrients,there are many plant-beneficial elements:silicon,aluminum,selenium,titanium,iodine,vanadium,cobalt,sodium,and rare earth elements.They are not essential for all plants,but some are crucial for specific plant species.However,the mechanisms of action of many beneficial elements are still unclear,and products containing beneficial elements have not been widely accepted and used by the public.This review systematically summarizes the current knowledge of plant-beneficial elements.Most importantly,we offer suggestions for future research on beneficial elements,which include integrating cross-disciplinary and innovative technologies,expanding the scope of application and elemental spies,broadening the spatial and temporal scales of research,incorporating beneficial elements into the soil health evaluation system,and shifting from single to multi-element applications.In the future,research on beneficial elements should be closely centered around“mechanism+application”to meet the ever-increasing demands driven by population growth,improve human health,tackle environmental challenges,and promote rural economic development.展开更多
Existing quantitative trait locus(QTL)mapping had low efficiency in identifying small-effect and closely linked QTL-by-environment interactions(QEIs)in recombinant inbred lines(RILs),especially in the era of global cl...Existing quantitative trait locus(QTL)mapping had low efficiency in identifying small-effect and closely linked QTL-by-environment interactions(QEIs)in recombinant inbred lines(RILs),especially in the era of global climate change.To address this challenge,here we integrate the compressed variance component mixed model with our GCIM to propose 3vGCIM for identifying QEIs in RILs,and extend 3vGCIM-random to 3vGCIM-fixed.3vGCIM integrates genome-wide scanning with machine learning,significantly improving power.In the mixed full model,we consider all possible effects and control for all possible polygenic backgrounds.In simulation studies,3vGCIM exhibits higher power(∼92.00%),higher accuracy of the estimates for QTL position(∼1.900 cM2)and effect(∼0.050),and lower false positive rate(∼0.48‰)and false negative rate(<8.10%)in three environments of 300 RILs each than ICIM(47.57%;3.607 cM2,0.583;2.81‰;52.43%)and MCIM(60.30%;5.279 cM2,0.274;2.17‰;39.70%).In the real data analysis of rice yield-related traits in 240 RILs,3vGCIM mines more known genes(57–60)and known gene-by-environment interactions(GEIs)(14–19)and candidate GEIs(21–23)than ICIM(27,2,and 7),and MCIM(21,1,and 3),especially in small-effect and linked QTLs and QEIs.This makes 3vGCIM a powerful and sensitive tool for QTL mapping and molecular QTL mapping.展开更多
Transitioning from outcrossing to self-fertilization is a widespread reproductive strategy in plants,especially in environments where pollination is limited.Despite its prevalence,this transition has rarely been exami...Transitioning from outcrossing to self-fertilization is a widespread reproductive strategy in plants,especially in environments where pollination is limited.Despite its prevalence,this transition has rarely been examined using transplant experiments,and previous studies have overlooked the contribution of the male parent in elucidating mating diversity.In this study,six transplanted populations were generated to investigate the relationship of the pollination environment with plant mating patterns and fecundity in Primula oreodoxa,a species that exhibits both distyly(predominantly outcrossing)and homostyly(predominantly selfing),based on data from 3582 individuals and 11 SSR markers.Homostylous plants had fruit and seed sets comparable to those of distylous plants at lower elevations but exhibited a clear reproductive advantage at higher elevations,particularly compared with the S morph.As elevation increased,the populational selfing rates increased,and the genetic diversity among the progeny was reduced.Furthermore,the visitation frequency of long-tongued pollinators was negatively and positively correlated with the selfing rate and number of mates,respectively,in the L and S morphs.In contrast,short-tongued pollinator visitation showed opposite correlations with the selfing rate and number of mates in homostylous morphs.In most populations,individuals functioned consistently as both female and male,and mating occurred randomly,suggesting a breakdown of the distyly polymorphism.Overall,our results provide experimental validation of the reproductive advantages of homostyly at high elevations by revealing that pollinator visitation shapes the selfing rate and mating diversity within populations,potentially driving the divergence of mating systems along environmental gradients.展开更多
Despite Morocco's reliance on sunflower as an oilseed crop,little is known about its agronomic performance when sown in autumn or early winter.This knowledge gap is critical,as spring-sown varieties have shown dec...Despite Morocco's reliance on sunflower as an oilseed crop,little is known about its agronomic performance when sown in autumn or early winter.This knowledge gap is critical,as spring-sown varieties have shown declining performance in recent years under intensifying climate stress.Therefore,targeted breeding strategies could discover genotypes suitable for autumn or early winter sowing,with cold tolerance as a key selection criterion.Currently,‘Ichraq'is the only autumn-planted sunflower variety officially registered in Morocco,although efforts to release additional tolerant varieties are underway.This study evaluated 31 genotypes(MGB1to MGB31)selected from various environments under autumn planting conditions and conserved in the Moroccan Gene Bank.These genotypes were planted in early winter at a mountainous site known for its pronounced winter cold.Eighteen Morphological,physiological and agronomic parameters including initial vigor,leaf area,seed yield,oil content etc.,were assessed using both univariate and multivariate statistical approaches.Analysis of variance revealed significant genotypic differences across most traits,indicating substantial genetic variation.Notably,seed oil content ranged from 23.28%(MGB26)to 43.88%(MGB5),and seed yield from1400 kg/ha(MGB7)to 5400 kg/ha(MGB8).Principal component analysis(PCA)identified that the first principal component,accounting for over 24%of the total phenotypic variance,exhibits a strong positive loading of yield-related traits and chlorophyll content,while displaying a pronounced negative loading for oil content variables.This opposing gradient indicates a clear trade-off between vegetative productivity and oil accumulation across the evaluated genotypes.Hierarchical cluster analysis resolved the germplasm into two principal clusters with high within-group similarity,each further partitioned into relatively homogeneous subgroups.Notably,several genotypes outperformed the control variety Ichraq,underscoring their potential for autumn or early winter cultivation.Nonetheless,essential multi-environment trials remain to validate their phenotypic stability and to ascertain their value as genetic resources for sunflower breeding programs in Morocco and other Mediterranean agro-ecosystems.展开更多
Reproductive diapause is an insect survival strategy in which reproduction temporarily halts in response to adverse environmental changes.This process is characterized by arrested ovarian development and lipid accumul...Reproductive diapause is an insect survival strategy in which reproduction temporarily halts in response to adverse environmental changes.This process is characterized by arrested ovarian development and lipid accumulation in females.A reduction in juvenile hormone(JH)biosynthesis is known to initiate reproductive diapause,but its regulatory mechanism remains unclear.Seven up(Svp),a transcription factor from the nuclear receptor family,plays a crucial role in various developmental processes in insects.In this study,using the cabbage beetle Colaphellus bowringi as a model,we observed higher expression of Svp in the heads of female adults under reproductive photoperiodic conditions(short-day[SD])compared to diapause conditions(long-day[LD]).RNA interference-mediated knockdown of Svp in SD females induced typical diapause phenotypes,including ovarian arrest and lipid accumulation.The application of methoprene(ME),a JH receptor agonist,reversed these diapause phenotypes and restored reproduction,indicating that Svp’s regulation of reproductive diapause is dependent on JH signaling.Additionally,Svp knockdown led to the downregulation of JH pathway genes and a reduction in JH titers.Further evidence suggested that Svp regulates the expression of JHAMT1,a critical gene in JH biosynthesis,which determines diapause entry in C.bowringi.These findings suggest that diapause-inducing photoperiods suppress Svp expression,blocking JH production and triggering diapause.This work reveals a critical transcription factor that regulates reproductive diapause initiation through modulating JH production,providing a potential target for controlling pests capable of entering reproductive diapause.展开更多
Toxic heavy metal and metalloid(THMM)contamination poses a major global challenge,threatening human health and sustainable agriculture.The crucial role of the Cytochrome P450(CYP)gene family in plant tolerance to THMM...Toxic heavy metal and metalloid(THMM)contamination poses a major global challenge,threatening human health and sustainable agriculture.The crucial role of the Cytochrome P450(CYP)gene family in plant tolerance to THMMs has been recently highlighted,but there is still a lack of comprehensive understanding,especially in relation to metabolites.This study delved into the identification of CYP genes that are linked to the tolerance mechanisms of plants in response to heavy metal stress.The findings highlight the significant metabolic pathways that contribute to this resilience,using rice and Arabidopsis as exemplars.THMM exposure changed CYP gene expression in plants,and THMM antidotes mitigated its downregulation and that of flavonoid biosynthesis genes.CYP genes involved in THMM responses were predominantly enriched in the pathways associated with flavonoid synthesis,indicating functional adaptations to distinct stresses.Notably,anthocyanin(Ant)accumulation,a type of flavonoid,affected the uptake of various heavy metals in Brassica rapa,with flavonoid biosynthesis-associated genes correlating with Cd or As tolerance and Ant content.These findings highlight the critical importance of flavonoid metabolism and the intricate network of biosynthesis genes in bolstering plant resilience against heavy metal stress.This enhanced understanding paves the way for significant advancements in phytoremediation technologies,offering innovative solutions for soil and water decontamination.展开更多
The global burden of cancer,with over 19 million new cases annually,underscores the urgent need for effective therapies.Among the most promising anticancer compounds is camptothecin(CPT),a monoterpene alkaloid predomi...The global burden of cancer,with over 19 million new cases annually,underscores the urgent need for effective therapies.Among the most promising anticancer compounds is camptothecin(CPT),a monoterpene alkaloid predominantly derived from Nothapodytes species.Despite its significantpharmaceutical value,the exploitation of such Threatened Plant Species with Widespread Distribution(TPSWD),particularly driven by the global demand for natural compounds in anticancer therapies,presents a paradox in which their widespread distribution fails to ensure their secure conservation status.Furthermore,the lack of in-depth biogeographic and systematic studies complicates efforts to balance resource utilization with biodiversity preservation.The asymmetric distribution of CPT within plant taxa,along with limited knowledge of its biosynthetic pathways and the enzymes and genes involved,further hampers sustainable production.Here,we review the current knowledge on the production and protection of Nothapodytes,focusing on their plant resources,active ingredients,and natural drug derivatives.We also explore strategies for rescuing and sustainably utilizing Nothapodytes,including biotechnological advancements and integrated conservation practices.Finally,we propose future directions to address conservation challenges,ensuring a sustainable supply of CPT while safeguarding these TPSWD species.展开更多
Plants constitute nearly 80%of the planet’s total biomass(Bar-On et al.,2018);however,this vital group is experiencing severe threats,and recent evaluations indicate that approximately 45%of the world's described...Plants constitute nearly 80%of the planet’s total biomass(Bar-On et al.,2018);however,this vital group is experiencing severe threats,and recent evaluations indicate that approximately 45%of the world's described plant species are at risk of extinction(Bachman et al.,2024).The number of plant extinctions has increased by 60%in the last 100 years(Di Marco et al.,2017).Over the past 250 years,571 plant species have gone extinct—more than twice the combined total of extinct birds,mammals,and amphibians(217 species)(Briggs,2019).展开更多
Nicotinamide mononucleotide(NMN),a precursor in nicotinamide adenine dinucleotide(NAD)biosynthesis,has long been recognized for its pivotal role in medicine.Recent investigations have suggested its potential as a plan...Nicotinamide mononucleotide(NMN),a precursor in nicotinamide adenine dinucleotide(NAD)biosynthesis,has long been recognized for its pivotal role in medicine.Recent investigations have suggested its potential as a plant immunity inducer for controlling fungal diseases.However,whether NMN confers plant broad-spectrum resistance against diverse phytopathogens,and its underlying mechanisms remain ambiguous.In this study,we investigate the effect of NMN against multiple phytopathogens in tobacco.Our results demonstrate that tobacco pretreated with NMN exhibits enhanced resistance against Ralstonia solanacearum CQPS-1,Pseudomonas syringae DC3000ΔhopQ1-1,Phytophthora parasitica,and tobacco mosaic virus(TMV).NMN displays effectiveness within the concentration range of 50–600μmol L^(–1),with75μmol L^(–1)NMN exhibiting the most pronounced effect.The impact of NMN pretreatment could persist for up to 10 days.Beyond tobacco,NMN pretreatment enhances disease resistance in tomato and pepper plants against diverse pathogens,underscoring NMN’s capacity to confer broad-spectrum disease resistance in crops.Moreover,RT-qPCR analysis reveals that NMN significantly upregulates the expression of the pattern-triggered immunity(PTI)marker gene NbCYP71D20 and salicylic acid(SA)marker gene NbPR1a.This suggests that NMN enhances plant resistance by inducing both PTI and SA-mediated immunity.Interestingly,the positive impact of NMN on plant disease resistance is not significantly compromised in both NMN adenylyltransferase(NMNAT)-silenced plants and NAD receptor mutant lecrk-I.8,suggesting the existence of NAD-independent signaling pathways for NMN-induced plant immunity.In conclusion,our study establishes that the bioactive molecule NMN imparts broad-spectrum disease resistance in plants,offering a simple,environmental-friendly,and promising strategy for safeguarding crops against diverse phytopathogens.These findings also provide valuable insights for future in-depth studies into the functional mechanisms of NMN.展开更多
基金supported by grants from the National Natural Science Foundation of China(32270290)the Shanghai Engineering Research Center of Plant Germplasm Resources(17DZ2252700).
文摘Plants deploy a two-layered immune system:pathogen-associated molecular pattern(PAMP)-triggered immunity(PTl)and effector-triggered immunity(ETI).While PTI is initiated by cell surface receptors,ETI relies on intracellular NLR receptors that recognize pathogen effectors(Jones et al.,2024).The nucleoporin CONSTITUTIVE EXPRESSER OF PATHOGENESIS-RELATED GENES 5(CPR5)is a key negative regulator of ETI.CPR5 integrates nuclear transport,cell cycle control,and RNA processing to suppress immune signaling(Wang et al.,2014;Gu et al.,2016;Peng et al.,2022).Recent work revealed that CPR5 also modulates immunity through another nucleoporin,GUANYLATE-BINDING PROTEIN-LIKE 3(GBPL3),which interaCtS with PWWP-DOMAIN INTERACTOR OF POLYCOMBS1(PWO1),a key component of the chromatin-associated methyltransferase POLYCOMB REPRESSIVE COMPLEX 2(PRC2)(Reimann et al.,2023;Pan et al.,2025).These findings suggest unexplored roles for chromatin remodeling in the CPR5-mediated immunity.
基金supported by the Ministry of Science and Technology of China(2015FY210300)the National Natural Science Foundation of China(31420103911)the National Animal Collection Resource Center.
文摘A new flatid genus in the tribe Selizini(Hemiptera:Fulgoromorpha:Flatidae)from southwestern China is proposed:Wattlea Peng,Ai&Zhang gen.nov.based on W.distincta Ai,Peng&Zhang sp.nov.and W.wandingensis Peng,Ai&Zhang sp.nov.from Yunnan,China.A third new species in a different genus,Zecheuna curva Ai,Peng&Zhang sp.nov.from Hainan,China,is described.Diagnoses,detailed descriptions,and illustrations are provided for each taxon,as well as comparisons with closely-related genera.
基金funded by National Natural Science Foundation of China(grant no.32301870 to Chen Lin)Natural Science Foundation of Jiangsu Province(grant no.BK20230568 to Chen Lin)+3 种基金the Jiangsu Provincial Agricultural Science and Technology Independent Innovation Fund(grant no.CX(24)3124 to Chen Lin)Outstanding Ph.D.Programin Yangzhou(grant no.YZLYJFJH2022YXBS147 to Chen Lin)the General Project of Basic Scientific Research to colleges and universities in Jiangsu Province(grant no.22KJB210019 toChen Lin)the Priority Academic Program Development of Jiangsu Higher Education Institutions is greatly acknowledged.
文摘Glutathione S-transferases (GSTs) represent a large and diverse enzyme family ubiquitously distributed across the plant kingdom. These proteins catalyze the conjugation of glutathione (GSH) with electrophilic substrates in response to various stress conditions. Beyond their role in stress adaptation, certain GSTs are integral regulators of plant growth and development, contributing to a range of physiological processes. Most GST proteins exhibit dual enzymatic activities, functioning as both transferases and peroxidases, which enables their involvement in diverse cellular processes, including detoxification and stress responses. Recent advancements, particularly in X-ray crystallography, have enabled detailed structural analysis of GST proteins, significantly enhancing our understanding of their biological functions. This review offers a comprehensive overview of the classification and structural characteristics of GSTs in plants. It also highlights recent findings on their roles in plant growth and development, cell signaling, catalytic transport, and stress tolerance. Furthermore, key scientific challenges related to GSTs are discussed, focusing on their potential applications in agriculture. These insights aim to facilitate the screening of functional GST genes and support molecular breeding efforts across diverse crop species.
基金supported by the Guangdong Basic and Applied Basic Research Foundation (2025A1515012679)Open Fund of Shanghai Key Laboratory of Plant Functional Genomics and Resources (PFGR202502)
文摘Licochalcone A(LCA)is a characteristic compound in licorice Glycyrrhiza inflata and is widely utilized in pharmaceutical and cosmetic industries.However,the biosynthetic pathway and regulatory mechanisms of LCA remain poorly understood.In this study,we first found the accumulation of LCA is induced by methyl jasmonate(MeJA).Given that MYB transcriptional factors are well-documented as key regulators of flavonoid biosynthesis,we identified a total of 147 GiR2R3-MYB genes in G.inflata,which were classified into 28 subgroups.The chromosome distributions,sequence characteristics,gene structures,duplication events and cis-acting elements were also investigated.Through integrated analysis of GiR2R3-MYBs expression patterns across different tissues and under MeJA treatment,along with phylogenetic relationship,we identified GiMYB76—a MeJA-inducible MYB transcription factor—as a potential regulator of LCA accumulation.Functional validation showed that transgenic hairy roots overexpressing GiMYB76 exhibited a significant increase in LCA content.DAP-seq analysis of GiMYB76 revealed potential target genes involved in flavonoid biosynthesis regulation.Subsequent promoter activity assay verified that GiMYB76 can bind to the promoter and activate the expression of GiCHS4.Consistently,overexpression of GiCHS4 in G.inflata hairy roots also significantly enhanced LCA production.This study not only clarifies that GiMYB76 transcriptionally activated GiCHS4 to promote LCA biosynthesis but also provides valuable insights for basic research on licorice and the development of related industries.
基金funding from Deutsche Forschungsgemeinschaft (DFG)supported by an MCIN Ry C Programme MCIN/ AEI/10.13039/501100011033+2 种基金by the ‘European Union Next Generation EU/PRTR’ under grant no. RYC2021-032345-Isupported by the AEI (grant no. PID2019-107463RJ-I00/ AEI/10.13039/501100011033)the Regional Research and Development Programme of the Government of Navarre (call 2019, project Nitro Healthy, PC068)
文摘Ammonium toxicity in plants remains poorly understood despite extensive research.While nitrate is known to benefit plant growth,the synergistic effects of nitrate in mitigating ammonium toxicity,even at low concentrations,are not fully elucidated.This review delves into the physiological and molecular nature of this phenomenon.To date,nitrate-dependent alleviation of ammonium toxicity is the result of cumulative consequences of the role of nitrate as a nutrient and signal in plant performance.The ability to counteract the ammonium-induced acidification through nitrate uptake and metabolism,the enhancement of potassium uptake as an essential nitrate counterion,and the nitratedependent signaling of key factors involved in ammonium assimilation,ROS scavenging,and growth hormone biosynthesis,are the most relevant hallmarks.In addition,evidence suggests that the availability of nitrate and ammonium has driven ecological selection in plants,determining current N preferences,and may have led to the selection of nitrate-dependent and ammonium-sensitive domesticated crops and the inefficient use of N fertilizers in agriculture.As ammonium toxicity limits N fertilization options and reduces agricultural yields,when it could be a more sustainable and cheaper alternative to nitrate,this review provides a better understanding of how plants use nitrate to counteract the problematic aspects of ammonium nutrition.
基金supported by the National Key R&D Program of China(2024YFE0113000)the Natural Science Foundation of Ningxia Province,China(2022AAC03241)the China Postdoctoral Science Foundation(2024M753573)。
文摘The Loxostege sticticalis(Lepidoptera:Pyralidae)is a major migratory pest of agriculture and animal husbandry in Asia and Europe.Utilizing plant volatile organic compounds(pVOCs)as attractants for monitoring and controlling pests is considered an environmentally friendly and effective method.However,limited knowledge exists regarding applying pVOCs to manage L.sticticalis.Here,volatile compounds released by Chenopodium album,Setaria viridis,and Medicago sativa,the three preferred oviposition plants for L.sticticalis females,were collected using dynamic headspace sampling techniques.A total of 55 distinct compounds were identified through gas chromatography-mass spectrometry(GC-MS),and 16 compounds in the concentration range from 0.001 to 100µgµL–1 elicited consistently enhanced electrophysiological responses in both male and female L.sticticalis.Subsequently,the attraction potential of four bioactive compounds-linalool,cis-anethole,trans-2-hexenal,and 1-octen-3-ol-were further confirmed by indoor behavioral bioassays.The blends of linalool,cis-anethole,trans-2-hexenal,and 1-octen-3-ol mixed at ratios of 5:1:5:10(formulation No.25)and 5:1:1:10(formulation No.21)were highly attractive to L.sticticalis adults.Field-trapping assays indicated that lure No.2 baited with formulation 21 demonstrated superior efficacy in field trapping.These findings suggest that pVOC-based attractants can be effectively employed for monitoring and mass trapping L.sticticalis adults,providing insights into the development of botanical attractants.
基金supported by the National Natural Science Foundation of China(Grant Nos.32072630,32372774,and U22A20499)the earmarked fund for CARS(Grant No.CARS-19-01A).
文摘Tea plant(Camellia sinensis(L.)O.Kuntze)is a cold-sensitive leaf-harvesting crop whose growth,yield,and processed tea quality are all inhibited by low temperatures.Therefore,identifying the regulatory genes involved in tea plant growth and freezing tolerance is crucial for genetic improvement.WRKY transcription factors regulate various plant processes,including growth and development,stress responses,and metabolite biosynthesis.However,the molecular network through which WRKY coordinates these pathways in tea plants remains unclear.In this study,we revealed that CsWRKY57L,a cold-inducible WRKY IIc subfamily member,positively regulated freezing tolerance by directly promoting flavonoid accumulation in tea plants.Transient suppression of CsWRKY57L weakened the freezing tolerance of tea plants by reducing flavonoid content and suppressing the C-repeat-binding factor(CBF)-cold-responsive(COR)gene pathway.In contrast,heterologous overexpression of CsWRKY57L in Arabidopsis had the opposite effect.Additionally,overexpression of CsWRKY57L inhibited reproductive development and accelerated senescence in Arabidopsis.Interaction analysis revealed that CsWRKY57L directly binds to the promoters of CsSWEET1a,CsSWEET15,and AtSWEET15,which encode sugar transporters essential for plant reproductive development,and inhibits their transcription.Overall,the study revealed a dual role of CsWRKY57L in promoting freezing tolerance via flavonoid biosynthesis and inhibiting reproductive development by regulating SWEETs expression.This study uncovers a novel mechanism whereby CsWRKY57L coordinately regulates both stress responses and growth in tea plants,providing a molecular basis for breeding low-temperature-tolerant varieties with restricted reproductive development.
基金supported by the Lendület/Momentum Programme of the Hungarian Academy of Sciencesthe National Research, Development, and Innovation Office, Hungary (Grant Nos. LP2024/21 and K146791)+2 种基金Bayers fellowship program MEDHA and Department of Botany, University of Calicutthe financial assistance provided in the form of Junior Research Fellowship from the University Grants Commission (UGC), Indiathe financial assistance provided by the Council for Scientific and Industrial Research(CSIR), India
文摘Ascorbate(Asc),commonly known as vitamin C,is a vital molecule for plant growth,development,and stress resilience.It is also known to play a crucial role in various physiological processes,including photosynthesis,cell division,and differentiation.This article thoroughly explores the processes governing the metabolism of Asc in plants and its roles in physiological functions.It lays down a robust theoretical groundwork for delving into Asc production,transportation,functions,and its potential applications in stress alleviation and horticulture.Furthermore,recent studies indicate that Asc plays a role in regulating fruit development and affecting postharvest storage characteristics,thereby influencing fruit ripening and resilience to stress.Hence,there is a growing importance in studying the synthesis and utilization of Asc in plants.Although the critical role of Asc in controlling plant redox signals has been extensively studied,the precise mechanisms by which it manages cellular redox homeostasis to maintain the equilibrium between reactive oxygen scavenging and cell redox signaling remain elusive.This gap in knowledge presents fresh opportunities to explore how the production of Asc in plants is regulated and how plants react to environmental stressors.Furthermore,this article delves into the potential for a comprehensive investigation into the essential function of Asc in fruits,the development of Asc-rich fruits,and the enhancement of postharvest storage properties.
基金supported by the Major Project of Guizhou Provincial Science and Technology Program,China([2024]027)the High-Level Innovative Talents Project of Guizhou Province,China(GCC[2023]014)+1 种基金the Guizhou Provincial Tea Industry Technology System,China(GZCYCYJSTX-03)the Science and Technology Project of China Huaneng Group(HNKJ2022-H135)。
文摘Heat stress reduces theanine content in tea plants,but the underlying molecular mechanism remains unclear.In this study,a temperature gradient treatment(20℃,25℃,30℃,and 35℃)was performed to unveil the effect of heat stress on biosynthesis and accumulation of theanine.We found that heat stress induced metabolic changes,characterized by decreased theanine content and increased catechin levels.In addition,heat stress up-regulated the expression of the class B heat shock transcription factor gene CsHSFB2c,while significantly suppressing the transcription of key theanine biosynthetic genes CsTS1 and CsGS1.Functional studies showed that silencing CsHSFB2c increased theanine content,while its overexpression significantly decreased theanine levels.Consistent with these changes,silencing CsHSFB2c upregulated the expression of CsTS1 and CsGS1,while overexpression of CsHSFB2c downregulated their expression.Yeast one-hybrid(Y1H)and dual-luciferase reporter gene(Dual-LUC)assays showed that CsHSFB2c directly binds to the promoters of CsTS1 and CsGS1 and inhibits their expression.These results demonstrate that CsHSFB2c mediates heat-induced suppression of theanine biosynthesis by directly inhibiting the expression of CsTS1 and CsGS1.This study provides a theoretical basis for improving the heat resistance and quality of tea plants via molecular breeding.
基金The corresponding author is also deeply grateful to Ministry of Minority Affairs,Government of India,for providing financial assistance(MANF-JAM-99722)during his research work.
文摘Plant tissue culture represents an advanced biotechnological technique for propagating and conserving threatened plant species efficiently.This method enables the rapid production of genetically identical plants under controlled sterile laboratory conditions(in vitro).Its applications span forestry,horticulture,and,crucially,plant breeding.Nanoparticles have emerged as innovative tools to address limitations in conventional plant tissue culture,offering diverse functionalities based on their unique physicochemical properties.This review focuses on the utilization of nanotechnology in enhancing various aspects of plant tissue culture.Nanoparticles,such as silver and zinc oxide,have demonstrated significant roles as antimicrobial agents and anti-browning agents.They also serve as elicitors,stimulating callus proliferation,root elongation,rapid shoot formation,and the enhanced production of bioactive compounds on a large scale.Furthermore,nanoparticles contribute to mitigating oxidative stress within cells,thereby promoting increased callus formation,elongated roots,and elevated production of secondary metabolites.Their influence extends to somaclonal variation and genetic transformation processes within plant tissue culture.These contributions collectively underscore the potential of nanoparticles to foster more efficient,sustainable,and scalable biotechnological solutions in in vitro culture.The implications extend to reducing resource dependency and mitigating environmental impacts,positioning nanotechnology as a transformative approach in sustainable plant biotechnology.
文摘[Objective]To systematically isolate and purify the polysaccharide from the mycelium of Streptomyces rochei D74(SRP),elucidate its fine structure,and evaluate the effect of the purified polysaccharide fraction on the growth of Salvia miltiorrhiza hairy roots and the biosynthesis of tanshinones,along with the underlying mechanism.[Methods]The crude polysaccharide was extracted using hot water,which was followed by ethanol precipitation and deproteinization via the Sevag method.Further purification was performed using DEAE-52 anionexchange chromatography and Sephadex G-100 gel filtration chromatography.The physicochemical properties and structural features of the main active fraction,SRP-W-2,were systematically characterized by Fourier transform infrared spectroscopy(FTIR),high performance liquid chromatography-mass spectrometry(HPLC-MS),and nuclear magnetic resonance(NMR).The effects of SRP-W-2 on hairy root growth and the biosynthesis of tanshinones were assessed by measuring biomass,tanshinone content,and the expression levels of key biosynthetic genes.[Results]SRP-W-2 was obtained with a yield of 2.41%.It was primarily composed of glucose and galactose at a molar ratio of 12.53:1.Structural analysis revealed that the backbone of SRP-W-2 consisted of→4)-α-D-Glcp-(1→and→4)-α-D-Galp-(1→residues,with branching points at→4,6)-α-D-Glcp-(1→and→4,6)-α-D-Galp-(1→.The side chain was identified asα-D-Glcp-(1→4)-α-DGlcp-(1→.Bioactivity assays demonstrated that SRP-W-2 significantly enhanced both the biomass of S.miltiorrhiza hairy roots and the accumulation of tanshinones.After 15 d of treatment with 50 mg/L SRP-W-2,the dry weight of the hairy roots increased by 37.52%.Meanwhile,the content of cryptotanshinone(CT),dihydrotanshinone I(DT-I),tanshinone I(T-I),and tanshinone IIA(TIIA)was increased by 19.0-fold,6.4-fold,2.8-fold,and 4.8-fold,respectively.Gene expression analysis further indicated that SRP-W-2 up-regulated key genes involved in the tanshinone biosynthetic pathway,including HMGR,DXS,DXR,and GGPPS.[Conclusion]The polysaccharide fraction SRP-W-2 from S.rochei D74 simultaneously promoted the growth of S.miltiorrhiza hairy roots and the biosynthesis of tanshinones,demonstrating its potential as an effective elicitor.This study provided a new strategy for the utilization and development of S.miltiorrhiza resources.
基金jointly supported by grants from the National Natural Science Foundation of China(324B2064 and 32272799)the Fundamental Research Funds for the Central Universities,China(226-2024-00052)。
文摘Maintaining optimal crop nutritional levels is crucial for maximizing yield and enhancing stress resistance.In addition to the 17 essential nutrients,there are many plant-beneficial elements:silicon,aluminum,selenium,titanium,iodine,vanadium,cobalt,sodium,and rare earth elements.They are not essential for all plants,but some are crucial for specific plant species.However,the mechanisms of action of many beneficial elements are still unclear,and products containing beneficial elements have not been widely accepted and used by the public.This review systematically summarizes the current knowledge of plant-beneficial elements.Most importantly,we offer suggestions for future research on beneficial elements,which include integrating cross-disciplinary and innovative technologies,expanding the scope of application and elemental spies,broadening the spatial and temporal scales of research,incorporating beneficial elements into the soil health evaluation system,and shifting from single to multi-element applications.In the future,research on beneficial elements should be closely centered around“mechanism+application”to meet the ever-increasing demands driven by population growth,improve human health,tackle environmental challenges,and promote rural economic development.
基金supported by the National Natural Science Foundation of China(32270673 and 32470657).
文摘Existing quantitative trait locus(QTL)mapping had low efficiency in identifying small-effect and closely linked QTL-by-environment interactions(QEIs)in recombinant inbred lines(RILs),especially in the era of global climate change.To address this challenge,here we integrate the compressed variance component mixed model with our GCIM to propose 3vGCIM for identifying QEIs in RILs,and extend 3vGCIM-random to 3vGCIM-fixed.3vGCIM integrates genome-wide scanning with machine learning,significantly improving power.In the mixed full model,we consider all possible effects and control for all possible polygenic backgrounds.In simulation studies,3vGCIM exhibits higher power(∼92.00%),higher accuracy of the estimates for QTL position(∼1.900 cM2)and effect(∼0.050),and lower false positive rate(∼0.48‰)and false negative rate(<8.10%)in three environments of 300 RILs each than ICIM(47.57%;3.607 cM2,0.583;2.81‰;52.43%)and MCIM(60.30%;5.279 cM2,0.274;2.17‰;39.70%).In the real data analysis of rice yield-related traits in 240 RILs,3vGCIM mines more known genes(57–60)and known gene-by-environment interactions(GEIs)(14–19)and candidate GEIs(21–23)than ICIM(27,2,and 7),and MCIM(21,1,and 3),especially in small-effect and linked QTLs and QEIs.This makes 3vGCIM a powerful and sensitive tool for QTL mapping and molecular QTL mapping.
基金funded by grants from the National Natural Science Foundation of China (31800314,32370239,U160323)the foundation of South China Botanical Garden,Chinese Academy of Sciences (QNXM-06)to SYthe Doctoral Research Foundation of China West Normal University (412994)。
文摘Transitioning from outcrossing to self-fertilization is a widespread reproductive strategy in plants,especially in environments where pollination is limited.Despite its prevalence,this transition has rarely been examined using transplant experiments,and previous studies have overlooked the contribution of the male parent in elucidating mating diversity.In this study,six transplanted populations were generated to investigate the relationship of the pollination environment with plant mating patterns and fecundity in Primula oreodoxa,a species that exhibits both distyly(predominantly outcrossing)and homostyly(predominantly selfing),based on data from 3582 individuals and 11 SSR markers.Homostylous plants had fruit and seed sets comparable to those of distylous plants at lower elevations but exhibited a clear reproductive advantage at higher elevations,particularly compared with the S morph.As elevation increased,the populational selfing rates increased,and the genetic diversity among the progeny was reduced.Furthermore,the visitation frequency of long-tongued pollinators was negatively and positively correlated with the selfing rate and number of mates,respectively,in the L and S morphs.In contrast,short-tongued pollinator visitation showed opposite correlations with the selfing rate and number of mates in homostylous morphs.In most populations,individuals functioned consistently as both female and male,and mating occurred randomly,suggesting a breakdown of the distyly polymorphism.Overall,our results provide experimental validation of the reproductive advantages of homostyly at high elevations by revealing that pollinator visitation shapes the selfing rate and mating diversity within populations,potentially driving the divergence of mating systems along environmental gradients.
文摘Despite Morocco's reliance on sunflower as an oilseed crop,little is known about its agronomic performance when sown in autumn or early winter.This knowledge gap is critical,as spring-sown varieties have shown declining performance in recent years under intensifying climate stress.Therefore,targeted breeding strategies could discover genotypes suitable for autumn or early winter sowing,with cold tolerance as a key selection criterion.Currently,‘Ichraq'is the only autumn-planted sunflower variety officially registered in Morocco,although efforts to release additional tolerant varieties are underway.This study evaluated 31 genotypes(MGB1to MGB31)selected from various environments under autumn planting conditions and conserved in the Moroccan Gene Bank.These genotypes were planted in early winter at a mountainous site known for its pronounced winter cold.Eighteen Morphological,physiological and agronomic parameters including initial vigor,leaf area,seed yield,oil content etc.,were assessed using both univariate and multivariate statistical approaches.Analysis of variance revealed significant genotypic differences across most traits,indicating substantial genetic variation.Notably,seed oil content ranged from 23.28%(MGB26)to 43.88%(MGB5),and seed yield from1400 kg/ha(MGB7)to 5400 kg/ha(MGB8).Principal component analysis(PCA)identified that the first principal component,accounting for over 24%of the total phenotypic variance,exhibits a strong positive loading of yield-related traits and chlorophyll content,while displaying a pronounced negative loading for oil content variables.This opposing gradient indicates a clear trade-off between vegetative productivity and oil accumulation across the evaluated genotypes.Hierarchical cluster analysis resolved the germplasm into two principal clusters with high within-group similarity,each further partitioned into relatively homogeneous subgroups.Notably,several genotypes outperformed the control variety Ichraq,underscoring their potential for autumn or early winter cultivation.Nonetheless,essential multi-environment trials remain to validate their phenotypic stability and to ascertain their value as genetic resources for sunflower breeding programs in Morocco and other Mediterranean agro-ecosystems.
基金funded by the National Natural Science Foundation of China,China(grant no.32472543).
文摘Reproductive diapause is an insect survival strategy in which reproduction temporarily halts in response to adverse environmental changes.This process is characterized by arrested ovarian development and lipid accumulation in females.A reduction in juvenile hormone(JH)biosynthesis is known to initiate reproductive diapause,but its regulatory mechanism remains unclear.Seven up(Svp),a transcription factor from the nuclear receptor family,plays a crucial role in various developmental processes in insects.In this study,using the cabbage beetle Colaphellus bowringi as a model,we observed higher expression of Svp in the heads of female adults under reproductive photoperiodic conditions(short-day[SD])compared to diapause conditions(long-day[LD]).RNA interference-mediated knockdown of Svp in SD females induced typical diapause phenotypes,including ovarian arrest and lipid accumulation.The application of methoprene(ME),a JH receptor agonist,reversed these diapause phenotypes and restored reproduction,indicating that Svp’s regulation of reproductive diapause is dependent on JH signaling.Additionally,Svp knockdown led to the downregulation of JH pathway genes and a reduction in JH titers.Further evidence suggested that Svp regulates the expression of JHAMT1,a critical gene in JH biosynthesis,which determines diapause entry in C.bowringi.These findings suggest that diapause-inducing photoperiods suppress Svp expression,blocking JH production and triggering diapause.This work reveals a critical transcription factor that regulates reproductive diapause initiation through modulating JH production,providing a potential target for controlling pests capable of entering reproductive diapause.
基金supported by the Guizhou Provincial Science and Technology Project(Grant No.[2022]091).
文摘Toxic heavy metal and metalloid(THMM)contamination poses a major global challenge,threatening human health and sustainable agriculture.The crucial role of the Cytochrome P450(CYP)gene family in plant tolerance to THMMs has been recently highlighted,but there is still a lack of comprehensive understanding,especially in relation to metabolites.This study delved into the identification of CYP genes that are linked to the tolerance mechanisms of plants in response to heavy metal stress.The findings highlight the significant metabolic pathways that contribute to this resilience,using rice and Arabidopsis as exemplars.THMM exposure changed CYP gene expression in plants,and THMM antidotes mitigated its downregulation and that of flavonoid biosynthesis genes.CYP genes involved in THMM responses were predominantly enriched in the pathways associated with flavonoid synthesis,indicating functional adaptations to distinct stresses.Notably,anthocyanin(Ant)accumulation,a type of flavonoid,affected the uptake of various heavy metals in Brassica rapa,with flavonoid biosynthesis-associated genes correlating with Cd or As tolerance and Ant content.These findings highlight the critical importance of flavonoid metabolism and the intricate network of biosynthesis genes in bolstering plant resilience against heavy metal stress.This enhanced understanding paves the way for significant advancements in phytoremediation technologies,offering innovative solutions for soil and water decontamination.
基金supported by the National Key R&D Program of China(2024YFF1306700)the Key Project of Basic Research of Yunnan Province,China(202301AS070001)the Regional Innovative Development Joint Fund of NSFC(U23A20149).
文摘The global burden of cancer,with over 19 million new cases annually,underscores the urgent need for effective therapies.Among the most promising anticancer compounds is camptothecin(CPT),a monoterpene alkaloid predominantly derived from Nothapodytes species.Despite its significantpharmaceutical value,the exploitation of such Threatened Plant Species with Widespread Distribution(TPSWD),particularly driven by the global demand for natural compounds in anticancer therapies,presents a paradox in which their widespread distribution fails to ensure their secure conservation status.Furthermore,the lack of in-depth biogeographic and systematic studies complicates efforts to balance resource utilization with biodiversity preservation.The asymmetric distribution of CPT within plant taxa,along with limited knowledge of its biosynthetic pathways and the enzymes and genes involved,further hampers sustainable production.Here,we review the current knowledge on the production and protection of Nothapodytes,focusing on their plant resources,active ingredients,and natural drug derivatives.We also explore strategies for rescuing and sustainably utilizing Nothapodytes,including biotechnological advancements and integrated conservation practices.Finally,we propose future directions to address conservation challenges,ensuring a sustainable supply of CPT while safeguarding these TPSWD species.
基金support of the ORG.one project of Oxford Nanopore Technologies(ONT),the Rufford Grants(45249-1)the Idea Wild Grants(Project ID-KJOSINDI0125-00)the Mohamed Bin Zyed Species Conservation(MBZ)(GEF Grant no-240535253)Funds in our efforts to conserve threatened trees in the Western Ghats Biodiversity Hotspot Forest regions.
文摘Plants constitute nearly 80%of the planet’s total biomass(Bar-On et al.,2018);however,this vital group is experiencing severe threats,and recent evaluations indicate that approximately 45%of the world's described plant species are at risk of extinction(Bachman et al.,2024).The number of plant extinctions has increased by 60%in the last 100 years(Di Marco et al.,2017).Over the past 250 years,571 plant species have gone extinct—more than twice the combined total of extinct birds,mammals,and amphibians(217 species)(Briggs,2019).
基金supported by the Technology Innovation Leading Program of Shaanxi,China(2023QYPY2-01)the National Natural Science Foundation of China(32072399,32302296,and 32372483)+1 种基金the Fundamental Research Funds for the Central Universities(GK202201017 and GK202207024)the Program of Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests,China(MIMCP-202203)。
文摘Nicotinamide mononucleotide(NMN),a precursor in nicotinamide adenine dinucleotide(NAD)biosynthesis,has long been recognized for its pivotal role in medicine.Recent investigations have suggested its potential as a plant immunity inducer for controlling fungal diseases.However,whether NMN confers plant broad-spectrum resistance against diverse phytopathogens,and its underlying mechanisms remain ambiguous.In this study,we investigate the effect of NMN against multiple phytopathogens in tobacco.Our results demonstrate that tobacco pretreated with NMN exhibits enhanced resistance against Ralstonia solanacearum CQPS-1,Pseudomonas syringae DC3000ΔhopQ1-1,Phytophthora parasitica,and tobacco mosaic virus(TMV).NMN displays effectiveness within the concentration range of 50–600μmol L^(–1),with75μmol L^(–1)NMN exhibiting the most pronounced effect.The impact of NMN pretreatment could persist for up to 10 days.Beyond tobacco,NMN pretreatment enhances disease resistance in tomato and pepper plants against diverse pathogens,underscoring NMN’s capacity to confer broad-spectrum disease resistance in crops.Moreover,RT-qPCR analysis reveals that NMN significantly upregulates the expression of the pattern-triggered immunity(PTI)marker gene NbCYP71D20 and salicylic acid(SA)marker gene NbPR1a.This suggests that NMN enhances plant resistance by inducing both PTI and SA-mediated immunity.Interestingly,the positive impact of NMN on plant disease resistance is not significantly compromised in both NMN adenylyltransferase(NMNAT)-silenced plants and NAD receptor mutant lecrk-I.8,suggesting the existence of NAD-independent signaling pathways for NMN-induced plant immunity.In conclusion,our study establishes that the bioactive molecule NMN imparts broad-spectrum disease resistance in plants,offering a simple,environmental-friendly,and promising strategy for safeguarding crops against diverse phytopathogens.These findings also provide valuable insights for future in-depth studies into the functional mechanisms of NMN.
