The present work investigated the efficiency of leaf reflectance indices in the identification of Capsicum annuum L. var. annuum resistant to anthracnose in the fruit. Twenty-five F<sub>5:6</sub> families ...The present work investigated the efficiency of leaf reflectance indices in the identification of Capsicum annuum L. var. annuum resistant to anthracnose in the fruit. Twenty-five F<sub>5:6</sub> families originating from contrasting parents were assessed;the parents were accession UENF 2285 (susceptible to anthracnose) and accession UENF 1381, a hot pepper resistant to anthracnose in the fruit. The experiment was carried out in an experimental field in Campos dos Goytacazes, Rio de Janeiro, Brazil, between May and October of 2021. The treatments were arranged in a randomized block design, with three replications and five plants per plot. Fifteen LRIs were estimated using a CI-710 portable mini leaf spectrometer. The assessments covered all plant growth after flowering, and a total of six assessments were performed at 15-days intervals, beginning at 35 and ending 120 days after flowering (DAFs). Analysis of variance in a split-plot scheme was performed, as were tests of mean groupings and principal components analysis (PCA). The best period for evaluating leaf reflectance indices in C. annuum var. annuum is 120 days after flowering. The leaf reflectance indices PRI, CNDVI and Ctr2 stood out as effective in distinguishing between resistant and susceptible genotypes.展开更多
Genomic selection,the application of genomic prediction(GP)models to select candidate individuals,has significantly advanced in the past two decades,effectively accelerating genetic gains in plant breeding.This articl...Genomic selection,the application of genomic prediction(GP)models to select candidate individuals,has significantly advanced in the past two decades,effectively accelerating genetic gains in plant breeding.This article provides a holistic overview of key factors that have influenced GP in plant breeding during this period.We delved into the pivotal roles of training population size and genetic diversity,and their relationship with the breeding population,in determining GP accuracy.Special emphasis was placed on optimizing training population size.We explored its benefits and the associated diminishing returns beyond an optimum size.This was done while considering the balance between resource allocation and maximizing prediction accuracy through current optimization algorithms.The density and distribution of single-nucleotide polymorphisms,level of linkage disequilibrium,genetic complexity,trait heritability,statistical machine-learning methods,and non-additive effects are the other vital factors.Using wheat,maize,and potato as examples,we summarize the effect of these factors on the accuracy of GP for various traits.The search for high accuracy in GP—theoretically reaching one when using the Pearson’s correlation as a metric—is an active research area as yet far from optimal for various traits.We hypothesize that with ultra-high sizes of genotypic and phenotypic datasets,effective training population optimization methods and support from other omics approaches(transcriptomics,metabolomics and proteomics)coupled with deep-learning algorithms could overcome the boundaries of current limitations to achieve the highest possible prediction accuracy,making genomic selection an effective tool in plant breeding.展开更多
A steady rise in the overall population is creating an overburden on crops due to their global demand.On the other hand,given the current climate change and population growth,agricultural practices established during ...A steady rise in the overall population is creating an overburden on crops due to their global demand.On the other hand,given the current climate change and population growth,agricultural practices established during the Green Revolution are no longer viable.Consequently,innovative practices are the prerequisite of the time struggle with the rising global food demand.The potential of nanotechnology to reduce the phytotoxic effects of these ecological restrictions has shown significant promise.Nanoparticles(NPs)typically enhance plant resilience to stressors by fortifying the physical barrier,optimizing photosynthesis,stimulating enzymatic activity for defense,elevating the concentration of stress-resistant compounds,and activating the expression of genes associated with defense mechanisms.In this review,we thoroughly cover the uptake and translocations of NPs crops and their potential valuable functions in enhancing plant growth and development at different growth stages.Additionally,we addressed how NPs improve plant resistance to biotic and abiotic stress.Generally,this review presents a thorough understanding of the significance of NPs in plants and their prospective value for plant antioxidant and crop development.展开更多
Gerbera,a popular commercial cut flower with vibrant and striking colors has gained immense popularity in the floriculture industry.They are widely cultivated in various regions,making them available throughout the ye...Gerbera,a popular commercial cut flower with vibrant and striking colors has gained immense popularity in the floriculture industry.They are widely cultivated in various regions,making them available throughout the year.As a better alternative to conventional propagation methods(via seeds and rhizomes),plant tissue culture serves as way to avail large-scale,uniform,disease-free plantlets for commercial cultivation as well as to develop novel genotypes.In addition,it ensures production of healthy plantlets throughout the year in limited space.Based on the plant tissue culture techniques,the in vitro polyploidization,mutagenesis,and genetic transformation pave a path for creation of variation and eventually enhancing the ornamental traits to address the consumers’preferences and also facilitates in developing stress tolerant lines thereby minimizing the losses during cultivation,maintaining the quality of the flowers.This comprehensive review article presents an overview of the recent advancements on genetic improvement of gerbera via various cutting-edge plant tissue culture-based tools and techniques that contribute in enhancing the quality and efficiency of gerbera cultivation,meeting the demands of the floriculture industry while addressing the challenges of changing environment and resource limitations.展开更多
Molecular tools have drawn the attention ofmodern plant breeders for its great precision and superiority.As the global population is increasing gradually,food production should be enhanced to feed the growing populati...Molecular tools have drawn the attention ofmodern plant breeders for its great precision and superiority.As the global population is increasing gradually,food production should be enhanced to feed the growing population.Therefore,precise and fast breeding tools are becoming obvious.Moreover,climate change has become a critical issue in crop improvement.Advanced breeding methods are vital to combat the impact of climate change,including biotic and abiotic stresses.Major molecular techniques,such as‘CRISPR-Cas’mediated‘genome editing’,‘marker-assisted selection(MAS)’,‘whole genome sequencing’,‘RNAi’,transgenic approach,‘high-throughput phenotyping(HTP)’,mutation breeding,have been proven superior over traditional breeding in terms of precision,efficiency,and speed in developing stress-resistant improved varieties.This review explores the potential and superiority ofmolecular breeding methods and highlights the gaps(time,cost,efficiency,etc.)in traditional breeding methods,where modern breeding programs,asmentioned,are effective.Furthermore,this reviewwill focus on the necessity of keymodern plant breeding techniques as a foundation for sustainable farming practices to address emerging environmental challenges,ensure food security,and improve the yield and quality of crops.展开更多
Indian mustard is recognized as a resilient and economically important oilseed crop.However,its potential remains untapped due to the limited availability of short-duration,high-yielding varieties capable of out-compe...Indian mustard is recognized as a resilient and economically important oilseed crop.However,its potential remains untapped due to the limited availability of short-duration,high-yielding varieties capable of out-competing other rabi crops.Considering this notion,we have evaluated twenty-one F_(2) and six BC1F1 populations derived from seven diversified parents of Brassica juncea following a Randomized Complete Block Design at Sher-e-Bangla Agricultural University.Based on key agronomic traits,the genetic components,heterosis,inbreeding depression,and gene action were studied to select early maturing and high-yielding populations.The percentage of heterosis was manifested in various cross-combinations,including P4×P6(91.45%for yield per plant)and P5×P6(28.52%for thousand seed weight),emerging as promising candidates for increasing productivity while managing negative inbreeding effects.Conversely,significant inbreeding depression was noted in traits like days to siliquae maturity and yield,particularly in crosses,P1×P2(6.29%)and P3×P5(21.74%),underscoring the need for careful selection in breeding programs to mitigate these effects.Variance analysis indicated that both additive and non-additive genetic interactions play a pivotal role in the inheritance patterns of the traits of interest.Among the six backcrosses,one promising line was(P5×P6)×P5,demonstrating early maturity(107.00 DAS)with improved seed yield(12.47 g).This combination exhibited the potential for enhancing the adaptability and productivity by maintaining the maturity index and accelerating yield.Furthermore,significant phenotypic variation across yield-contributing traits was notable,whereas thousand seed weight and yield per plant showed high broad-sense and narrow-sense of heritability.Besides,positive correlations between seed yield and its attributing traits were noted,suggesting potential avenues for selection breeding.Collectively,the ob-tained findings enhance the understanding of genetic mechanisms underlying heterosis and inbreeding depres-sion in B.juncea,providing insights and effective strategies for developing superior cultivars with optimized agronomic traits.展开更多
The ability of plants to tolerate cold is a complex process.When temperatures drop or freeze,plant tissues can develop ice,which dehydrates the cells.However,plants can protect themselves by preventing ice formation.T...The ability of plants to tolerate cold is a complex process.When temperatures drop or freeze,plant tissues can develop ice,which dehydrates the cells.However,plants can protect themselves by preventing ice formation.This intricate response to cold stress is regulated by hormones,photoperiod,light,and various factors,in addition to genetic influences.In autumn,plants undergo morphological,physiological,biochemical,and molecular changes to prepare for the low temperatures of winter.Understanding cellular stress responses is crucial for genetic manipulation aimed at enhancing cold resistance.Early autumn frosts or late spring chills can cause significant damage to plants,making it essential to adapt in autumn to survive winter conditions.While the general process of acclimatization is similar across many plant species,variations exist depending on the specific type of plant and regional conditions.Different plant organs exhibit varying degrees of damage from cold stress,and by applying agricultural principles,potential damage can be largely controlled.Timely reinforcement and stress prevention can minimize cold-related damage.Research has shown that in temperate climates,low temperatures restrict plant growth and yield.However,the intricate structural systems involved remain poorly understood.Over the past decade,studies have focused on the molecular mechanisms that enable plants to adapt to and resist cold stress.The gene signaling system is believed to play a crucial role in cold adaptation,and researchers have prioritized this area in their investigations.This study critically examines plant responses to cold stress through physiological adaptations,including calcium signaling dynamics,membrane lipid modifications,and adjustments in antioxidant systems.These mechanisms activate downstream gene expression and molecular functions,leading to key resistance strategies.Additionally,we explore the regulatory roles of endogenous phytohormones and secondary metabolites in cold stress responses.This review aims to enhance our foundational understanding of the mechanisms behind plant cold adaptation.展开更多
The genus Beta encompasses economically important root crops such as sugar and table beet.A Beta diversity set including the wild relative B.vulgaris ssp.maritima was grown in the field,and a large phenotypic diversit...The genus Beta encompasses economically important root crops such as sugar and table beet.A Beta diversity set including the wild relative B.vulgaris ssp.maritima was grown in the field,and a large phenotypic diversity was observed.The genomes of 290 accessions were sequenced,and more than 10 million high-quality SNPs were employed to study genetic diversity.A genome-wide association study was performed,and marker-trait associations were found for nine phenotypic traits.The candidate gene within the M locus controlling monogermity on chromosome 4 was previously unknown.The most significant association for monogermity was identified at the end of chromosome 4.Within this region,a non-synonymous mutation within the zinc-finger domain of the WIP2 gene co-segregated with monogermity.This gene plays a regulatory role in AGL8/FUL in Arabidopsis.Intriguingly,commercial hybrids are in a heterozygous state at this position.Thus,the long-sought gene for monogermity was identified in this study.Red and yellow pigmentation due to betalain accumulation in shoots and roots is an important characteristic of table and leaf beets.The strongest associations were found upstream or downstream of two genes encoding Cytochrome P450 and anthocyanin MYB-like transcription factor proteins involved in betalain biosynthesis.Significant associations for Cercospora leaf spot resistance were identified on chromosomes 1,2,7,and 9.The associated regions harbor genes encoding proteins with leucinerich repeats and nucleotide binding sites whose homologs are major constituents of plant-pathogen defense.展开更多
In addition to the negative consequences of climate change,sucking pest complexes severely limited cotton yields in the recent past.Although the damage caused by bollworms was much reduced by utilizing Bt cotton,the e...In addition to the negative consequences of climate change,sucking pest complexes severely limited cotton yields in the recent past.Although the damage caused by bollworms was much reduced by utilizing Bt cotton,the emergence of sucking pests(such as aphids,thrips,and whiteflies)poses a serious threat to cotton production,as they reduce lint yield by 40%–60%finally.Additionally,these pests also caused yield losses by spreading viral diseases.Promoting innovative and thorough control methods is necessary to counter the threat posed by these sucking pests.Such initiatives necessitate a multifaceted strategy that combines next-generation breeding technology and pest management techniques to produce novel cotton cultivars that are resistant to sucking pests.The discovery of novel genes and regulatory factors linked to cotton’s resistance to sucking pests will be possible by the combination of next-generation breeding technologies and omics approaches and employing those tools on special resistant donors.Continuous research aimed at understanding the genetic basis of insect resistance and improving integrated pest management(IPM)techniques is crucial to the sustainability and resilience of cotton cropping systems.To this end,a sustainable and viable strategy to protect cotton fields from sucking pests is outlined.展开更多
Global warming is making plants more susceptible to heat stress.Hence,adjustments to crop production systems are required for global food security.Heat stress(HS)poses a threat to the quality of ecosystems and global ...Global warming is making plants more susceptible to heat stress.Hence,adjustments to crop production systems are required for global food security.Heat stress(HS)poses a threat to the quality of ecosystems and global food security due to its adverse effects on plant development.The degree to which HS affects physiological disruptions,physical harm,and biochemical changes at various growth stages directly correlates with its effects on physiological functions,plant growth,and crop production.One promising approach is soil modification using biochar,which enhances soil health and promotes the development of microbial communities,ultimately improving plant heat tolerance.Biochar enhances soil structure,improves moisture retention,and increases nutrient availability in hot weather,thereby promoting plant growth and enhancing crop yields.Additionally,biochar,with its porous structure and ability to provide a liming effect,increases the diversity and activity of soil microbes,thereby fostering advantageous symbiotic relationships.These microbial communities support nutrient cycling,root growth,and general soil health,strengthening biochar’s position as a long-term solution for climate-resilient farming.Earlier research concentrated on the connection between biochar and heat stress or microbial populations;however,this review uniquely combines all three elements,providing a fresh viewpoint on their interrelated functions in enhancing plant adaptability.Furthermore,this study demonstrates the potential of biochar as a sustainable component for improving soil and supporting crops that adapt to heat stress.It examines the processes underlying these interactions and provides recommendations for future research strategies.展开更多
The analysis of combining ability and heterosis is very important in enhancing the yield and oil quality of sunflowers under adverse conditions,and it reveals the potential of the parents and the mechanism of gene act...The analysis of combining ability and heterosis is very important in enhancing the yield and oil quality of sunflowers under adverse conditions,and it reveals the potential of the parents and the mechanism of gene action.In this study,twenty-one hybrids were developed by crossing seven cytoplasmic male sterile(CMS)lines with three restorer lines and evaluated for agronomic and quality traits.Highly significant general combining ability(GCA)and specific combining ability(SCA)effects were observed,confirming the role of both additive and non-additive gene actions.Among the tested crosses,A-42×R-86,A-92×R-86,and A-92×R-114 exhibited the greatest heterotic advantage,with seed yields exceeding 340 kg ha^(−1) over the better parent,oil contents above 19%,and 100-seed weights greater than 27 g.The hybrid A-92×R-114 was particularly notable for its elevated oleic acid level and balanced fatty acid profile,making it a strong candidate for premium oilseed production.In contrast,hybrids like A-20×R-39 exhibited moderate heterosis and less quality superiority.The oleic-to-linoleic acid ratio,a key determinant of oil stability,was strongly controlled by genetic factors.Oil content was largely influenced by additive effects,whereas yield heterosis was predominantly governed by non-additive effects.Overall,A-42×R-86 and A-92×R-114 emerged as the most promising hybrids,combining yield benefits with improved oil quality,and offering practical guidance for parental selection in sunflower breeding programs.展开更多
Plants produce many amino acid derivatives(AADs).Some have been used by humans as medicines and nutrients,but many also act as phytochemical signals in plant growth and stress tolerance.The fluctuating ecological envi...Plants produce many amino acid derivatives(AADs).Some have been used by humans as medicines and nutrients,but many also act as phytochemical signals in plant growth and stress tolerance.The fluctuating ecological environment poses a constant challenge to plant growth and development,and also presents significant obstacles to agricultural productivity.Plant AADs hold substantial potential for agricultural applications to increase plant resilience against diverse biological and environmental pressures.In this review,we present recent advances in elucidating the biological roles of plant AADs in plant growth and stress tolerance and outline strategies for discovering novel AADs and their regulatory networks in crops.The review aims to gain new insights into the functional properties of AADs in regulating plant growth and stress responses,which provides a valuable foundation for developing innovative AADbased strategies to improve crop performance and resilience facing the ever-changing environment in the future.展开更多
Light and nitrogen(N)are two critically environmental factors essential for plant survival,as they constitute the fundamental molecular framework of plant cells and significantly influence patterns of plant growth and...Light and nitrogen(N)are two critically environmental factors essential for plant survival,as they constitute the fundamental molecular framework of plant cells and significantly influence patterns of plant growth and development.Light is the driving force behind photosynthesis,a process that converts light energy into chemical energy stored as sugars.Additionally,light acts as a direct signal that can modulate plant morphogenesis and structural development.Nitrogen,as the most crucial mineral nutrient for plants,is a component of numerous biomolecules.It also functions as a signaling molecule,regulating plant growth and development.Moreover,light and nitrogen directly regulate the balance of carbon(C)and N within plants,affecting numerous biochemical reactions and various physiological processes.This review focuses on the interactions between light and nitrogen in physiological,metabolic,and molecular levels.We will also discuss the regulatory networks and mechanisms through which light and nitrogen influence C and N absorption and metabolism in plants.展开更多
The yield potential of rice is seriously affected by heat stress due to climate change. Since rice is a staple food globally, it is imperative to develop heat-resistant rice varieties. Thus, a thorough understanding o...The yield potential of rice is seriously affected by heat stress due to climate change. Since rice is a staple food globally, it is imperative to develop heat-resistant rice varieties. Thus, a thorough understanding of the complex molecular mechanisms underlying heat tolerance and the impact of high temperatures on various critical stages of the crop is needed. Adoption of both conventional and innovative breeding strategies offers a long-term advantage over other methods, such as agronomic practices, to counter heat stress. In this review, we summarize the effects of heat stress, regulatory pathways for heat tolerance, phenotyping strategies, and various breeding methods available for developing heat-tolerant rice. We offer perspectives and knowledge to guide future research endeavors aimed at enhancing the ability of rice to withstand heat stress and ultimately benefit humanity.展开更多
The jasmonate ZIM-domain(JAZ)family of proteins serves as co-receptors and transcriptional repressors of jasmonic acid(JA)in plants.Their functional diversity and multiple roles make them important components of the r...The jasmonate ZIM-domain(JAZ)family of proteins serves as co-receptors and transcriptional repressors of jasmonic acid(JA)in plants.Their functional diversity and multiple roles make them important components of the regulatory network of JA and other hormonal signaling pathways.In this review,we provide an overview of the latest findings on JAZ family proteins and emphasize their roles in plant growth and development,and response to biotic and abiotic stress,along with their underlying mechanisms.Moreover,existing challenges and future applications are outlined with the aim of offering a reference for further research on JAZ proteins in the context of plant physiology.展开更多
Oenothera speciosa, belonging to thermophilous plant, cannot overwinter in Beijing. To enhance the overwintering rate of Oenothera speciosa, the seeds were treated through silico ion implantation (SII), with five vari...Oenothera speciosa, belonging to thermophilous plant, cannot overwinter in Beijing. To enhance the overwintering rate of Oenothera speciosa, the seeds were treated through silico ion implantation (SII), with five various fluence ranges (1 × 109 - 1 × 1011 ions/cm2) of 40 MeV and four various fluence ranges (1 × 1010 - 5 × 1011 ions/cm2) of 35 MeV, respectively. M1 generations of various SII-treated Oenothera speciosa lines can overwinter, and the highest overwinter rate (41.3%) was observed in Oenothera speciosa lines treated with 35 MeV and fluence 5 × 1010 ions/cm2. M2 and M3 generations of all treated lines were able to overwinter smoothly. The results indicated that SII treatment can enhance the cold-resistance of Oenothera speciosa heritably. Furthermore, physiological indexes including relative electrical conductivity, MDA contents and proline contents of SII-treated Oenothera speciosa pot seedlings were detected after low temperature stress. The results revealed that relative electrical conductivities and MDA contents of M1, M2 and M3 generations of SII-treated Oenothera speciosa plants were lower than that of control, whereas the proline contents were higher than control in the LJ°C cold stress. Taken together, the cold resistance of SII-treated Oenothera speciosa plants was improved, which made it possible to be used as a perennial flower in landscaping in Beijing.展开更多
Lodging is more than just plants falling over;it incurs significant economic losses for farmers leading to a decrease in both yield and quality of the final produce.Human management practices,such as dense sowing,exce...Lodging is more than just plants falling over;it incurs significant economic losses for farmers leading to a decrease in both yield and quality of the final produce.Human management practices,such as dense sowing,excessive nitrogen fertilizer applications,inappropriate sowing dates,and upland rice cultivation,exacerbate the risk of lodging in rice.While breeders have developed high-yielding rice varieties utilizing the sd1 gene,relying solely on this gene is insufficient to enhance lodging resistance.Identifying the traits that contribute to lodging resistance is crucial.Key factors include biochemical,anatomical,and morphological traits,such as the levels of lignin,cellulose,hemicellulose,silicon,and potassium,along with the number and area of vascular bundles and the thickness,diameter,and length of the culm.Moreover,markers associated with lodging-related genes,like SCM2,SCM3,SCM4,and prl4,can be utilized effectively in marker-assisted backcrossing to develop rice varieties with desirable culm traits.This literature review aims to aid rice breeders in addressing the issue of lodging by examining traits that influence lodging resistance,developing phenotyping strategies for these traits,identifying suitable instrumentation,exploring methods for screening lodging-resistant plants,understanding the mathematical relationships involved,and considering molecular breeding aspects for pyramiding genes related to lodging.展开更多
The impact of epigenetic modifications like DNA methylation on plant phenotypes has expanded the possibilities for crop development.DNA methylation plays a part in the regulation of both the chromatin structure and ge...The impact of epigenetic modifications like DNA methylation on plant phenotypes has expanded the possibilities for crop development.DNA methylation plays a part in the regulation of both the chromatin structure and gene expression,and the enzyme involved,DNA methyltransferase,executes the methylation process within the plant genome.By regulating crucial biological pathways,epigenetic changes actively contribute to the creation of the phenotype.Therefore,epigenome editing may assist in overcoming some of the drawbacks of genome editing,which can have minor off-target consequences and merely facilitate the loss of a gene’s function.These drawbacks include gene knockout,which can have such off-target effects.This review provides examples of several molecular characteristics of DNA methylation,as well as some plant physiological processes that are impacted by these epigenetic changes in the plants.We also discuss how DNA alterations might be used to improve crops and meet the demands of sustainable and environmentally-friendly farming.展开更多
Global efforts to address malnutrition and hidden hunger, particularly prevalent in low- and middle-income countries, have intensified, with a focus on enhancing the nutritional content of staple crops like rice. Desp...Global efforts to address malnutrition and hidden hunger, particularly prevalent in low- and middle-income countries, have intensified, with a focus on enhancing the nutritional content of staple crops like rice. Despite serving as a staple for over half of the world's population, rice falls short in meeting daily nutritional requirements, especially for iron(Fe) and zinc(Zn). Genetic resources, such as wild rice species and specific rice varieties, offer promising avenues for enhancing Fe and Zn content. Additionally, molecular breeding approaches have identified key genes and loci associated with Fe and Zn accumulation in rice grains. This review explores the genetic resources and molecular mechanisms underlying Fe and Zn accumulation in rice grains. The functional genomics involved in Fe uptake, transport, and distribution in rice plants have revealed key genes such as OsFRO1, OsIRT1, and OsNAS3. Similarly, genes associated with Zn uptake and translocation, including OsZIP11 and OsNRAMP1, have been identified. Transgenic approaches, leveraging transporter gene families and genome editing technologies, offer promising avenues for enhancing Fe and Zn content in rice grains. Moreover, strategies for reducing phytic acid(PA) content, a known inhibitor of mineral bioavailability, have been explored, including the identification of low-PA mutants and natural variants. The integration of genomic information, including whole-genome resequencing and pan-genome analyses, provides valuable insights into the genetic basis of micronutrient traits and facilitates targeted breeding efforts. Functional genomics studies have elucidated the molecular mechanisms underlying Fe uptake and translocation in rice. Furthermore, transgenic and genome editing techniques have shown promise in enhancing Fe and Zn content in rice grains through the manipulation of key transporter genes. Overall, the integration of multi-omics approaches holds significant promise for addressing global malnutrition and hidden hunger by enhancing the nutritional quality of rice, thereby contributing to improved food and nutritional security worldwide.展开更多
文摘The present work investigated the efficiency of leaf reflectance indices in the identification of Capsicum annuum L. var. annuum resistant to anthracnose in the fruit. Twenty-five F<sub>5:6</sub> families originating from contrasting parents were assessed;the parents were accession UENF 2285 (susceptible to anthracnose) and accession UENF 1381, a hot pepper resistant to anthracnose in the fruit. The experiment was carried out in an experimental field in Campos dos Goytacazes, Rio de Janeiro, Brazil, between May and October of 2021. The treatments were arranged in a randomized block design, with three replications and five plants per plot. Fifteen LRIs were estimated using a CI-710 portable mini leaf spectrometer. The assessments covered all plant growth after flowering, and a total of six assessments were performed at 15-days intervals, beginning at 35 and ending 120 days after flowering (DAFs). Analysis of variance in a split-plot scheme was performed, as were tests of mean groupings and principal components analysis (PCA). The best period for evaluating leaf reflectance indices in C. annuum var. annuum is 120 days after flowering. The leaf reflectance indices PRI, CNDVI and Ctr2 stood out as effective in distinguishing between resistant and susceptible genotypes.
基金supported by SLU Grogrund(#SLU-LTV.2020.1.1.1-654)an Einar and Inga Nilsson Foundation grant.J.I.y.S.was supported by grant PID2021-123718OB-I00+4 种基金funded by MCIN/AEI/10.13039/501100011033by“ERDF A way of making Europe,”CEX2020-000999-S.R.R.V.supported by Novo Nordisk Fonden(0074727)SLU’s Centre for Biological ControlIn addition,J.I.y.S.and J.F.-G.were supported by the Beatriz Galindo Program BEAGAL 18/00115.
文摘Genomic selection,the application of genomic prediction(GP)models to select candidate individuals,has significantly advanced in the past two decades,effectively accelerating genetic gains in plant breeding.This article provides a holistic overview of key factors that have influenced GP in plant breeding during this period.We delved into the pivotal roles of training population size and genetic diversity,and their relationship with the breeding population,in determining GP accuracy.Special emphasis was placed on optimizing training population size.We explored its benefits and the associated diminishing returns beyond an optimum size.This was done while considering the balance between resource allocation and maximizing prediction accuracy through current optimization algorithms.The density and distribution of single-nucleotide polymorphisms,level of linkage disequilibrium,genetic complexity,trait heritability,statistical machine-learning methods,and non-additive effects are the other vital factors.Using wheat,maize,and potato as examples,we summarize the effect of these factors on the accuracy of GP for various traits.The search for high accuracy in GP—theoretically reaching one when using the Pearson’s correlation as a metric—is an active research area as yet far from optimal for various traits.We hypothesize that with ultra-high sizes of genotypic and phenotypic datasets,effective training population optimization methods and support from other omics approaches(transcriptomics,metabolomics and proteomics)coupled with deep-learning algorithms could overcome the boundaries of current limitations to achieve the highest possible prediction accuracy,making genomic selection an effective tool in plant breeding.
基金The authors extend their gratitude to the Deanship of Scientific Research(DSR),King Faisal University,Saudi Arabia,for funding the publication of this work(Project number:KFU250560).
文摘A steady rise in the overall population is creating an overburden on crops due to their global demand.On the other hand,given the current climate change and population growth,agricultural practices established during the Green Revolution are no longer viable.Consequently,innovative practices are the prerequisite of the time struggle with the rising global food demand.The potential of nanotechnology to reduce the phytotoxic effects of these ecological restrictions has shown significant promise.Nanoparticles(NPs)typically enhance plant resilience to stressors by fortifying the physical barrier,optimizing photosynthesis,stimulating enzymatic activity for defense,elevating the concentration of stress-resistant compounds,and activating the expression of genes associated with defense mechanisms.In this review,we thoroughly cover the uptake and translocations of NPs crops and their potential valuable functions in enhancing plant growth and development at different growth stages.Additionally,we addressed how NPs improve plant resistance to biotic and abiotic stress.Generally,this review presents a thorough understanding of the significance of NPs in plants and their prospective value for plant antioxidant and crop development.
基金funded by Department of Science&Technology and Biotechnology,Govt.of West Bengal,India[Sanction No.565(Sanc.)/STBT-13015/15/11/2021-ST SEC]。
文摘Gerbera,a popular commercial cut flower with vibrant and striking colors has gained immense popularity in the floriculture industry.They are widely cultivated in various regions,making them available throughout the year.As a better alternative to conventional propagation methods(via seeds and rhizomes),plant tissue culture serves as way to avail large-scale,uniform,disease-free plantlets for commercial cultivation as well as to develop novel genotypes.In addition,it ensures production of healthy plantlets throughout the year in limited space.Based on the plant tissue culture techniques,the in vitro polyploidization,mutagenesis,and genetic transformation pave a path for creation of variation and eventually enhancing the ornamental traits to address the consumers’preferences and also facilitates in developing stress tolerant lines thereby minimizing the losses during cultivation,maintaining the quality of the flowers.This comprehensive review article presents an overview of the recent advancements on genetic improvement of gerbera via various cutting-edge plant tissue culture-based tools and techniques that contribute in enhancing the quality and efficiency of gerbera cultivation,meeting the demands of the floriculture industry while addressing the challenges of changing environment and resource limitations.
文摘Molecular tools have drawn the attention ofmodern plant breeders for its great precision and superiority.As the global population is increasing gradually,food production should be enhanced to feed the growing population.Therefore,precise and fast breeding tools are becoming obvious.Moreover,climate change has become a critical issue in crop improvement.Advanced breeding methods are vital to combat the impact of climate change,including biotic and abiotic stresses.Major molecular techniques,such as‘CRISPR-Cas’mediated‘genome editing’,‘marker-assisted selection(MAS)’,‘whole genome sequencing’,‘RNAi’,transgenic approach,‘high-throughput phenotyping(HTP)’,mutation breeding,have been proven superior over traditional breeding in terms of precision,efficiency,and speed in developing stress-resistant improved varieties.This review explores the potential and superiority ofmolecular breeding methods and highlights the gaps(time,cost,efficiency,etc.)in traditional breeding methods,where modern breeding programs,asmentioned,are effective.Furthermore,this reviewwill focus on the necessity of keymodern plant breeding techniques as a foundation for sustainable farming practices to address emerging environmental challenges,ensure food security,and improve the yield and quality of crops.
基金funded by Bangladesh Academy of Sciences(BAS),and Fund ID:BAS-USDA SAU CR02.
文摘Indian mustard is recognized as a resilient and economically important oilseed crop.However,its potential remains untapped due to the limited availability of short-duration,high-yielding varieties capable of out-competing other rabi crops.Considering this notion,we have evaluated twenty-one F_(2) and six BC1F1 populations derived from seven diversified parents of Brassica juncea following a Randomized Complete Block Design at Sher-e-Bangla Agricultural University.Based on key agronomic traits,the genetic components,heterosis,inbreeding depression,and gene action were studied to select early maturing and high-yielding populations.The percentage of heterosis was manifested in various cross-combinations,including P4×P6(91.45%for yield per plant)and P5×P6(28.52%for thousand seed weight),emerging as promising candidates for increasing productivity while managing negative inbreeding effects.Conversely,significant inbreeding depression was noted in traits like days to siliquae maturity and yield,particularly in crosses,P1×P2(6.29%)and P3×P5(21.74%),underscoring the need for careful selection in breeding programs to mitigate these effects.Variance analysis indicated that both additive and non-additive genetic interactions play a pivotal role in the inheritance patterns of the traits of interest.Among the six backcrosses,one promising line was(P5×P6)×P5,demonstrating early maturity(107.00 DAS)with improved seed yield(12.47 g).This combination exhibited the potential for enhancing the adaptability and productivity by maintaining the maturity index and accelerating yield.Furthermore,significant phenotypic variation across yield-contributing traits was notable,whereas thousand seed weight and yield per plant showed high broad-sense and narrow-sense of heritability.Besides,positive correlations between seed yield and its attributing traits were noted,suggesting potential avenues for selection breeding.Collectively,the ob-tained findings enhance the understanding of genetic mechanisms underlying heterosis and inbreeding depres-sion in B.juncea,providing insights and effective strategies for developing superior cultivars with optimized agronomic traits.
文摘The ability of plants to tolerate cold is a complex process.When temperatures drop or freeze,plant tissues can develop ice,which dehydrates the cells.However,plants can protect themselves by preventing ice formation.This intricate response to cold stress is regulated by hormones,photoperiod,light,and various factors,in addition to genetic influences.In autumn,plants undergo morphological,physiological,biochemical,and molecular changes to prepare for the low temperatures of winter.Understanding cellular stress responses is crucial for genetic manipulation aimed at enhancing cold resistance.Early autumn frosts or late spring chills can cause significant damage to plants,making it essential to adapt in autumn to survive winter conditions.While the general process of acclimatization is similar across many plant species,variations exist depending on the specific type of plant and regional conditions.Different plant organs exhibit varying degrees of damage from cold stress,and by applying agricultural principles,potential damage can be largely controlled.Timely reinforcement and stress prevention can minimize cold-related damage.Research has shown that in temperate climates,low temperatures restrict plant growth and yield.However,the intricate structural systems involved remain poorly understood.Over the past decade,studies have focused on the molecular mechanisms that enable plants to adapt to and resist cold stress.The gene signaling system is believed to play a crucial role in cold adaptation,and researchers have prioritized this area in their investigations.This study critically examines plant responses to cold stress through physiological adaptations,including calcium signaling dynamics,membrane lipid modifications,and adjustments in antioxidant systems.These mechanisms activate downstream gene expression and molecular functions,leading to key resistance strategies.Additionally,we explore the regulatory roles of endogenous phytohormones and secondary metabolites in cold stress responses.This review aims to enhance our foundational understanding of the mechanisms behind plant cold adaptation.
基金the financial support from the German Research Foundation(Deutsche Forschungsgemeinschaft,DFG)—Project Number 400993799(Project 2 within the Research Training Group 2501 Translational Evolutionary Research,https://gepris.dfg.de/gepris/projekt/400993799)supported by the BMBF-funded de.NBI Cloud,part of the German Network for Bioinformatics(de.NBI)。
文摘The genus Beta encompasses economically important root crops such as sugar and table beet.A Beta diversity set including the wild relative B.vulgaris ssp.maritima was grown in the field,and a large phenotypic diversity was observed.The genomes of 290 accessions were sequenced,and more than 10 million high-quality SNPs were employed to study genetic diversity.A genome-wide association study was performed,and marker-trait associations were found for nine phenotypic traits.The candidate gene within the M locus controlling monogermity on chromosome 4 was previously unknown.The most significant association for monogermity was identified at the end of chromosome 4.Within this region,a non-synonymous mutation within the zinc-finger domain of the WIP2 gene co-segregated with monogermity.This gene plays a regulatory role in AGL8/FUL in Arabidopsis.Intriguingly,commercial hybrids are in a heterozygous state at this position.Thus,the long-sought gene for monogermity was identified in this study.Red and yellow pigmentation due to betalain accumulation in shoots and roots is an important characteristic of table and leaf beets.The strongest associations were found upstream or downstream of two genes encoding Cytochrome P450 and anthocyanin MYB-like transcription factor proteins involved in betalain biosynthesis.Significant associations for Cercospora leaf spot resistance were identified on chromosomes 1,2,7,and 9.The associated regions harbor genes encoding proteins with leucinerich repeats and nucleotide binding sites whose homologs are major constituents of plant-pathogen defense.
基金M/s.RASI Seeds Pvt.Ltd.,Attur,Tamil Nadu,India for their generous financial assistance in setting up a MAS study in cotton for genetic improvement of sucking pest resistance.
文摘In addition to the negative consequences of climate change,sucking pest complexes severely limited cotton yields in the recent past.Although the damage caused by bollworms was much reduced by utilizing Bt cotton,the emergence of sucking pests(such as aphids,thrips,and whiteflies)poses a serious threat to cotton production,as they reduce lint yield by 40%–60%finally.Additionally,these pests also caused yield losses by spreading viral diseases.Promoting innovative and thorough control methods is necessary to counter the threat posed by these sucking pests.Such initiatives necessitate a multifaceted strategy that combines next-generation breeding technology and pest management techniques to produce novel cotton cultivars that are resistant to sucking pests.The discovery of novel genes and regulatory factors linked to cotton’s resistance to sucking pests will be possible by the combination of next-generation breeding technologies and omics approaches and employing those tools on special resistant donors.Continuous research aimed at understanding the genetic basis of insect resistance and improving integrated pest management(IPM)techniques is crucial to the sustainability and resilience of cotton cropping systems.To this end,a sustainable and viable strategy to protect cotton fields from sucking pests is outlined.
文摘Global warming is making plants more susceptible to heat stress.Hence,adjustments to crop production systems are required for global food security.Heat stress(HS)poses a threat to the quality of ecosystems and global food security due to its adverse effects on plant development.The degree to which HS affects physiological disruptions,physical harm,and biochemical changes at various growth stages directly correlates with its effects on physiological functions,plant growth,and crop production.One promising approach is soil modification using biochar,which enhances soil health and promotes the development of microbial communities,ultimately improving plant heat tolerance.Biochar enhances soil structure,improves moisture retention,and increases nutrient availability in hot weather,thereby promoting plant growth and enhancing crop yields.Additionally,biochar,with its porous structure and ability to provide a liming effect,increases the diversity and activity of soil microbes,thereby fostering advantageous symbiotic relationships.These microbial communities support nutrient cycling,root growth,and general soil health,strengthening biochar’s position as a long-term solution for climate-resilient farming.Earlier research concentrated on the connection between biochar and heat stress or microbial populations;however,this review uniquely combines all three elements,providing a fresh viewpoint on their interrelated functions in enhancing plant adaptability.Furthermore,this study demonstrates the potential of biochar as a sustainable component for improving soil and supporting crops that adapt to heat stress.It examines the processes underlying these interactions and provides recommendations for future research strategies.
文摘The analysis of combining ability and heterosis is very important in enhancing the yield and oil quality of sunflowers under adverse conditions,and it reveals the potential of the parents and the mechanism of gene action.In this study,twenty-one hybrids were developed by crossing seven cytoplasmic male sterile(CMS)lines with three restorer lines and evaluated for agronomic and quality traits.Highly significant general combining ability(GCA)and specific combining ability(SCA)effects were observed,confirming the role of both additive and non-additive gene actions.Among the tested crosses,A-42×R-86,A-92×R-86,and A-92×R-114 exhibited the greatest heterotic advantage,with seed yields exceeding 340 kg ha^(−1) over the better parent,oil contents above 19%,and 100-seed weights greater than 27 g.The hybrid A-92×R-114 was particularly notable for its elevated oleic acid level and balanced fatty acid profile,making it a strong candidate for premium oilseed production.In contrast,hybrids like A-20×R-39 exhibited moderate heterosis and less quality superiority.The oleic-to-linoleic acid ratio,a key determinant of oil stability,was strongly controlled by genetic factors.Oil content was largely influenced by additive effects,whereas yield heterosis was predominantly governed by non-additive effects.Overall,A-42×R-86 and A-92×R-114 emerged as the most promising hybrids,combining yield benefits with improved oil quality,and offering practical guidance for parental selection in sunflower breeding programs.
基金funded by National Natural Science Foundation of China(32472034)Guangdong Province Natural Science Foundation(2022A1515010707)。
文摘Plants produce many amino acid derivatives(AADs).Some have been used by humans as medicines and nutrients,but many also act as phytochemical signals in plant growth and stress tolerance.The fluctuating ecological environment poses a constant challenge to plant growth and development,and also presents significant obstacles to agricultural productivity.Plant AADs hold substantial potential for agricultural applications to increase plant resilience against diverse biological and environmental pressures.In this review,we present recent advances in elucidating the biological roles of plant AADs in plant growth and stress tolerance and outline strategies for discovering novel AADs and their regulatory networks in crops.The review aims to gain new insights into the functional properties of AADs in regulating plant growth and stress responses,which provides a valuable foundation for developing innovative AADbased strategies to improve crop performance and resilience facing the ever-changing environment in the future.
基金supported by grants from Hainan Seed Laboratory(B21HJ0003)the National Natural Science Foundation of China(U23A20185)the Hainan Excellent Talent Team。
文摘Light and nitrogen(N)are two critically environmental factors essential for plant survival,as they constitute the fundamental molecular framework of plant cells and significantly influence patterns of plant growth and development.Light is the driving force behind photosynthesis,a process that converts light energy into chemical energy stored as sugars.Additionally,light acts as a direct signal that can modulate plant morphogenesis and structural development.Nitrogen,as the most crucial mineral nutrient for plants,is a component of numerous biomolecules.It also functions as a signaling molecule,regulating plant growth and development.Moreover,light and nitrogen directly regulate the balance of carbon(C)and N within plants,affecting numerous biochemical reactions and various physiological processes.This review focuses on the interactions between light and nitrogen in physiological,metabolic,and molecular levels.We will also discuss the regulatory networks and mechanisms through which light and nitrogen influence C and N absorption and metabolism in plants.
文摘The yield potential of rice is seriously affected by heat stress due to climate change. Since rice is a staple food globally, it is imperative to develop heat-resistant rice varieties. Thus, a thorough understanding of the complex molecular mechanisms underlying heat tolerance and the impact of high temperatures on various critical stages of the crop is needed. Adoption of both conventional and innovative breeding strategies offers a long-term advantage over other methods, such as agronomic practices, to counter heat stress. In this review, we summarize the effects of heat stress, regulatory pathways for heat tolerance, phenotyping strategies, and various breeding methods available for developing heat-tolerant rice. We offer perspectives and knowledge to guide future research endeavors aimed at enhancing the ability of rice to withstand heat stress and ultimately benefit humanity.
基金funded by the National Natural Science Foundation of China (32372156,32201838,32272157)the Natural Science Foundation of Guangdong Province (2024A1515011084,2023A1515012052,2023A1515012092)the Science and Technology Project of Guangzhou (2023A04J0749,2023A04J1452).
文摘The jasmonate ZIM-domain(JAZ)family of proteins serves as co-receptors and transcriptional repressors of jasmonic acid(JA)in plants.Their functional diversity and multiple roles make them important components of the regulatory network of JA and other hormonal signaling pathways.In this review,we provide an overview of the latest findings on JAZ family proteins and emphasize their roles in plant growth and development,and response to biotic and abiotic stress,along with their underlying mechanisms.Moreover,existing challenges and future applications are outlined with the aim of offering a reference for further research on JAZ proteins in the context of plant physiology.
文摘Oenothera speciosa, belonging to thermophilous plant, cannot overwinter in Beijing. To enhance the overwintering rate of Oenothera speciosa, the seeds were treated through silico ion implantation (SII), with five various fluence ranges (1 × 109 - 1 × 1011 ions/cm2) of 40 MeV and four various fluence ranges (1 × 1010 - 5 × 1011 ions/cm2) of 35 MeV, respectively. M1 generations of various SII-treated Oenothera speciosa lines can overwinter, and the highest overwinter rate (41.3%) was observed in Oenothera speciosa lines treated with 35 MeV and fluence 5 × 1010 ions/cm2. M2 and M3 generations of all treated lines were able to overwinter smoothly. The results indicated that SII treatment can enhance the cold-resistance of Oenothera speciosa heritably. Furthermore, physiological indexes including relative electrical conductivity, MDA contents and proline contents of SII-treated Oenothera speciosa pot seedlings were detected after low temperature stress. The results revealed that relative electrical conductivities and MDA contents of M1, M2 and M3 generations of SII-treated Oenothera speciosa plants were lower than that of control, whereas the proline contents were higher than control in the LJ°C cold stress. Taken together, the cold resistance of SII-treated Oenothera speciosa plants was improved, which made it possible to be used as a perennial flower in landscaping in Beijing.
文摘Lodging is more than just plants falling over;it incurs significant economic losses for farmers leading to a decrease in both yield and quality of the final produce.Human management practices,such as dense sowing,excessive nitrogen fertilizer applications,inappropriate sowing dates,and upland rice cultivation,exacerbate the risk of lodging in rice.While breeders have developed high-yielding rice varieties utilizing the sd1 gene,relying solely on this gene is insufficient to enhance lodging resistance.Identifying the traits that contribute to lodging resistance is crucial.Key factors include biochemical,anatomical,and morphological traits,such as the levels of lignin,cellulose,hemicellulose,silicon,and potassium,along with the number and area of vascular bundles and the thickness,diameter,and length of the culm.Moreover,markers associated with lodging-related genes,like SCM2,SCM3,SCM4,and prl4,can be utilized effectively in marker-assisted backcrossing to develop rice varieties with desirable culm traits.This literature review aims to aid rice breeders in addressing the issue of lodging by examining traits that influence lodging resistance,developing phenotyping strategies for these traits,identifying suitable instrumentation,exploring methods for screening lodging-resistant plants,understanding the mathematical relationships involved,and considering molecular breeding aspects for pyramiding genes related to lodging.
文摘The impact of epigenetic modifications like DNA methylation on plant phenotypes has expanded the possibilities for crop development.DNA methylation plays a part in the regulation of both the chromatin structure and gene expression,and the enzyme involved,DNA methyltransferase,executes the methylation process within the plant genome.By regulating crucial biological pathways,epigenetic changes actively contribute to the creation of the phenotype.Therefore,epigenome editing may assist in overcoming some of the drawbacks of genome editing,which can have minor off-target consequences and merely facilitate the loss of a gene’s function.These drawbacks include gene knockout,which can have such off-target effects.This review provides examples of several molecular characteristics of DNA methylation,as well as some plant physiological processes that are impacted by these epigenetic changes in the plants.We also discuss how DNA alterations might be used to improve crops and meet the demands of sustainable and environmentally-friendly farming.
文摘Global efforts to address malnutrition and hidden hunger, particularly prevalent in low- and middle-income countries, have intensified, with a focus on enhancing the nutritional content of staple crops like rice. Despite serving as a staple for over half of the world's population, rice falls short in meeting daily nutritional requirements, especially for iron(Fe) and zinc(Zn). Genetic resources, such as wild rice species and specific rice varieties, offer promising avenues for enhancing Fe and Zn content. Additionally, molecular breeding approaches have identified key genes and loci associated with Fe and Zn accumulation in rice grains. This review explores the genetic resources and molecular mechanisms underlying Fe and Zn accumulation in rice grains. The functional genomics involved in Fe uptake, transport, and distribution in rice plants have revealed key genes such as OsFRO1, OsIRT1, and OsNAS3. Similarly, genes associated with Zn uptake and translocation, including OsZIP11 and OsNRAMP1, have been identified. Transgenic approaches, leveraging transporter gene families and genome editing technologies, offer promising avenues for enhancing Fe and Zn content in rice grains. Moreover, strategies for reducing phytic acid(PA) content, a known inhibitor of mineral bioavailability, have been explored, including the identification of low-PA mutants and natural variants. The integration of genomic information, including whole-genome resequencing and pan-genome analyses, provides valuable insights into the genetic basis of micronutrient traits and facilitates targeted breeding efforts. Functional genomics studies have elucidated the molecular mechanisms underlying Fe uptake and translocation in rice. Furthermore, transgenic and genome editing techniques have shown promise in enhancing Fe and Zn content in rice grains through the manipulation of key transporter genes. Overall, the integration of multi-omics approaches holds significant promise for addressing global malnutrition and hidden hunger by enhancing the nutritional quality of rice, thereby contributing to improved food and nutritional security worldwide.
