The present work investigated the efficiency of leaf reflectance indices in the identification of Capsicum annuum L. var. annuum resistant to anthracnose in the fruit. Twenty-five F<sub>5:6</sub> families ...The present work investigated the efficiency of leaf reflectance indices in the identification of Capsicum annuum L. var. annuum resistant to anthracnose in the fruit. Twenty-five F<sub>5:6</sub> families originating from contrasting parents were assessed;the parents were accession UENF 2285 (susceptible to anthracnose) and accession UENF 1381, a hot pepper resistant to anthracnose in the fruit. The experiment was carried out in an experimental field in Campos dos Goytacazes, Rio de Janeiro, Brazil, between May and October of 2021. The treatments were arranged in a randomized block design, with three replications and five plants per plot. Fifteen LRIs were estimated using a CI-710 portable mini leaf spectrometer. The assessments covered all plant growth after flowering, and a total of six assessments were performed at 15-days intervals, beginning at 35 and ending 120 days after flowering (DAFs). Analysis of variance in a split-plot scheme was performed, as were tests of mean groupings and principal components analysis (PCA). The best period for evaluating leaf reflectance indices in C. annuum var. annuum is 120 days after flowering. The leaf reflectance indices PRI, CNDVI and Ctr2 stood out as effective in distinguishing between resistant and susceptible genotypes.展开更多
The present study investigated the genetic diversity of 24 germplasms of Polianthes tuberosa L.via 16 inter simple sequence repeat(ISSR)marker techniques.The research findings revealed that the ISSR markers presented ...The present study investigated the genetic diversity of 24 germplasms of Polianthes tuberosa L.via 16 inter simple sequence repeat(ISSR)marker techniques.The research findings revealed that the ISSR markers presented higher levels of band reproducibility andweremore efficient at clustering germplasms.Among the 16markers examined in this study,12 had a complete polymorphism rate of 100%.Themolecular analysis revealed a PICranging from0.079 to 0.373,with amean value of 0.30,whereas the range of themarker index was from0.0001 to 0.409,with an average value of 0.03,and the primer resolving power ranged from 0.173 to 4.173,with a mean value of 2.02.The UPGMA clustering dendrogram indicated that all 24 germplasms were grouped into three main clusters.The study revealed a variable range of tree distances between 0.185 and 0.621,with the highest tree distance(0.621)detected between germplasms BR-24 and BR-1.Through these studies,the dissimilarity among the germplasms was evaluated,and diverse parents were identified for further crop improvement programs.展开更多
Genomic selection,the application of genomic prediction(GP)models to select candidate individuals,has significantly advanced in the past two decades,effectively accelerating genetic gains in plant breeding.This articl...Genomic selection,the application of genomic prediction(GP)models to select candidate individuals,has significantly advanced in the past two decades,effectively accelerating genetic gains in plant breeding.This article provides a holistic overview of key factors that have influenced GP in plant breeding during this period.We delved into the pivotal roles of training population size and genetic diversity,and their relationship with the breeding population,in determining GP accuracy.Special emphasis was placed on optimizing training population size.We explored its benefits and the associated diminishing returns beyond an optimum size.This was done while considering the balance between resource allocation and maximizing prediction accuracy through current optimization algorithms.The density and distribution of single-nucleotide polymorphisms,level of linkage disequilibrium,genetic complexity,trait heritability,statistical machine-learning methods,and non-additive effects are the other vital factors.Using wheat,maize,and potato as examples,we summarize the effect of these factors on the accuracy of GP for various traits.The search for high accuracy in GP—theoretically reaching one when using the Pearson’s correlation as a metric—is an active research area as yet far from optimal for various traits.We hypothesize that with ultra-high sizes of genotypic and phenotypic datasets,effective training population optimization methods and support from other omics approaches(transcriptomics,metabolomics and proteomics)coupled with deep-learning algorithms could overcome the boundaries of current limitations to achieve the highest possible prediction accuracy,making genomic selection an effective tool in plant breeding.展开更多
Oenothera speciosa, belonging to thermophilous plant, cannot overwinter in Beijing. To enhance the overwintering rate of Oenothera speciosa, the seeds were treated through silico ion implantation (SII), with five vari...Oenothera speciosa, belonging to thermophilous plant, cannot overwinter in Beijing. To enhance the overwintering rate of Oenothera speciosa, the seeds were treated through silico ion implantation (SII), with five various fluence ranges (1 × 109 - 1 × 1011 ions/cm2) of 40 MeV and four various fluence ranges (1 × 1010 - 5 × 1011 ions/cm2) of 35 MeV, respectively. M1 generations of various SII-treated Oenothera speciosa lines can overwinter, and the highest overwinter rate (41.3%) was observed in Oenothera speciosa lines treated with 35 MeV and fluence 5 × 1010 ions/cm2. M2 and M3 generations of all treated lines were able to overwinter smoothly. The results indicated that SII treatment can enhance the cold-resistance of Oenothera speciosa heritably. Furthermore, physiological indexes including relative electrical conductivity, MDA contents and proline contents of SII-treated Oenothera speciosa pot seedlings were detected after low temperature stress. The results revealed that relative electrical conductivities and MDA contents of M1, M2 and M3 generations of SII-treated Oenothera speciosa plants were lower than that of control, whereas the proline contents were higher than control in the LJ°C cold stress. Taken together, the cold resistance of SII-treated Oenothera speciosa plants was improved, which made it possible to be used as a perennial flower in landscaping in Beijing.展开更多
Gerbera,a popular commercial cut flower with vibrant and striking colors has gained immense popularity in the floriculture industry.They are widely cultivated in various regions,making them available throughout the ye...Gerbera,a popular commercial cut flower with vibrant and striking colors has gained immense popularity in the floriculture industry.They are widely cultivated in various regions,making them available throughout the year.As a better alternative to conventional propagation methods(via seeds and rhizomes),plant tissue culture serves as way to avail large-scale,uniform,disease-free plantlets for commercial cultivation as well as to develop novel genotypes.In addition,it ensures production of healthy plantlets throughout the year in limited space.Based on the plant tissue culture techniques,the in vitro polyploidization,mutagenesis,and genetic transformation pave a path for creation of variation and eventually enhancing the ornamental traits to address the consumers’preferences and also facilitates in developing stress tolerant lines thereby minimizing the losses during cultivation,maintaining the quality of the flowers.This comprehensive review article presents an overview of the recent advancements on genetic improvement of gerbera via various cutting-edge plant tissue culture-based tools and techniques that contribute in enhancing the quality and efficiency of gerbera cultivation,meeting the demands of the floriculture industry while addressing the challenges of changing environment and resource limitations.展开更多
A steady rise in the overall population is creating an overburden on crops due to their global demand.On the other hand,given the current climate change and population growth,agricultural practices established during ...A steady rise in the overall population is creating an overburden on crops due to their global demand.On the other hand,given the current climate change and population growth,agricultural practices established during the Green Revolution are no longer viable.Consequently,innovative practices are the prerequisite of the time struggle with the rising global food demand.The potential of nanotechnology to reduce the phytotoxic effects of these ecological restrictions has shown significant promise.Nanoparticles(NPs)typically enhance plant resilience to stressors by fortifying the physical barrier,optimizing photosynthesis,stimulating enzymatic activity for defense,elevating the concentration of stress-resistant compounds,and activating the expression of genes associated with defense mechanisms.In this review,we thoroughly cover the uptake and translocations of NPs crops and their potential valuable functions in enhancing plant growth and development at different growth stages.Additionally,we addressed how NPs improve plant resistance to biotic and abiotic stress.Generally,this review presents a thorough understanding of the significance of NPs in plants and their prospective value for plant antioxidant and crop development.展开更多
Molecular tools have drawn the attention ofmodern plant breeders for its great precision and superiority.As the global population is increasing gradually,food production should be enhanced to feed the growing populati...Molecular tools have drawn the attention ofmodern plant breeders for its great precision and superiority.As the global population is increasing gradually,food production should be enhanced to feed the growing population.Therefore,precise and fast breeding tools are becoming obvious.Moreover,climate change has become a critical issue in crop improvement.Advanced breeding methods are vital to combat the impact of climate change,including biotic and abiotic stresses.Major molecular techniques,such as‘CRISPR-Cas’mediated‘genome editing’,‘marker-assisted selection(MAS)’,‘whole genome sequencing’,‘RNAi’,transgenic approach,‘high-throughput phenotyping(HTP)’,mutation breeding,have been proven superior over traditional breeding in terms of precision,efficiency,and speed in developing stress-resistant improved varieties.This review explores the potential and superiority ofmolecular breeding methods and highlights the gaps(time,cost,efficiency,etc.)in traditional breeding methods,where modern breeding programs,asmentioned,are effective.Furthermore,this reviewwill focus on the necessity of keymodern plant breeding techniques as a foundation for sustainable farming practices to address emerging environmental challenges,ensure food security,and improve the yield and quality of crops.展开更多
Indian mustard is recognized as a resilient and economically important oilseed crop.However,its potential remains untapped due to the limited availability of short-duration,high-yielding varieties capable of out-compe...Indian mustard is recognized as a resilient and economically important oilseed crop.However,its potential remains untapped due to the limited availability of short-duration,high-yielding varieties capable of out-competing other rabi crops.Considering this notion,we have evaluated twenty-one F_(2) and six BC1F1 populations derived from seven diversified parents of Brassica juncea following a Randomized Complete Block Design at Sher-e-Bangla Agricultural University.Based on key agronomic traits,the genetic components,heterosis,inbreeding depression,and gene action were studied to select early maturing and high-yielding populations.The percentage of heterosis was manifested in various cross-combinations,including P4×P6(91.45%for yield per plant)and P5×P6(28.52%for thousand seed weight),emerging as promising candidates for increasing productivity while managing negative inbreeding effects.Conversely,significant inbreeding depression was noted in traits like days to siliquae maturity and yield,particularly in crosses,P1×P2(6.29%)and P3×P5(21.74%),underscoring the need for careful selection in breeding programs to mitigate these effects.Variance analysis indicated that both additive and non-additive genetic interactions play a pivotal role in the inheritance patterns of the traits of interest.Among the six backcrosses,one promising line was(P5×P6)×P5,demonstrating early maturity(107.00 DAS)with improved seed yield(12.47 g).This combination exhibited the potential for enhancing the adaptability and productivity by maintaining the maturity index and accelerating yield.Furthermore,significant phenotypic variation across yield-contributing traits was notable,whereas thousand seed weight and yield per plant showed high broad-sense and narrow-sense of heritability.Besides,positive correlations between seed yield and its attributing traits were noted,suggesting potential avenues for selection breeding.Collectively,the ob-tained findings enhance the understanding of genetic mechanisms underlying heterosis and inbreeding depres-sion in B.juncea,providing insights and effective strategies for developing superior cultivars with optimized agronomic traits.展开更多
Phytic acid(PA), or myo-inositol 1,2,3,4,5,6-hexakisphosphate, is the main storage form of phosphorus(P) in seeds, accounting for 65% to 85% of their total P content. The negative charge of PA attracts metal cations, ...Phytic acid(PA), or myo-inositol 1,2,3,4,5,6-hexakisphosphate, is the main storage form of phosphorus(P) in seeds, accounting for 65% to 85% of their total P content. The negative charge of PA attracts metal cations, forming insoluble salts called phytates. These phytates, contain six negatively charged ions, can bind divalent cations such as Fe^(2+), Zn^(2+), Mg^(2+), and Ca^(2+), preventing their absorption in monogastric animals. To overcome P deficiency in non-ruminants, phytase is usually given as a supplement, which then results in excess P excretion, leading to environmental problems such as eutrophication. Improved fertilizer management, food processing techniques, and the development of low-PA crops through plant breeding are envisioned as effective ways to improve P-utilization and lessen the environmental impact while minimizing the effect of PA. A better understanding of the molecular and physiological basis of PA biosynthesis, grain PA distribution, the effects of genetic and environmental factors on PA accumulation, and methods to increase micronutrient bioavailability by lowering the effects of PA is essential for developing low-PA crops.展开更多
The ability of plants to tolerate cold is a complex process.When temperatures drop or freeze,plant tissues can develop ice,which dehydrates the cells.However,plants can protect themselves by preventing ice formation.T...The ability of plants to tolerate cold is a complex process.When temperatures drop or freeze,plant tissues can develop ice,which dehydrates the cells.However,plants can protect themselves by preventing ice formation.This intricate response to cold stress is regulated by hormones,photoperiod,light,and various factors,in addition to genetic influences.In autumn,plants undergo morphological,physiological,biochemical,and molecular changes to prepare for the low temperatures of winter.Understanding cellular stress responses is crucial for genetic manipulation aimed at enhancing cold resistance.Early autumn frosts or late spring chills can cause significant damage to plants,making it essential to adapt in autumn to survive winter conditions.While the general process of acclimatization is similar across many plant species,variations exist depending on the specific type of plant and regional conditions.Different plant organs exhibit varying degrees of damage from cold stress,and by applying agricultural principles,potential damage can be largely controlled.Timely reinforcement and stress prevention can minimize cold-related damage.Research has shown that in temperate climates,low temperatures restrict plant growth and yield.However,the intricate structural systems involved remain poorly understood.Over the past decade,studies have focused on the molecular mechanisms that enable plants to adapt to and resist cold stress.The gene signaling system is believed to play a crucial role in cold adaptation,and researchers have prioritized this area in their investigations.This study critically examines plant responses to cold stress through physiological adaptations,including calcium signaling dynamics,membrane lipid modifications,and adjustments in antioxidant systems.These mechanisms activate downstream gene expression and molecular functions,leading to key resistance strategies.Additionally,we explore the regulatory roles of endogenous phytohormones and secondary metabolites in cold stress responses.This review aims to enhance our foundational understanding of the mechanisms behind plant cold adaptation.展开更多
Faba bean,with its high protein yield and low nitrogen inputs,is a promising crop,but it suffers from low yield stability due to poor heat and drought adaptation.To improve yield performance,faba beans can be produced...Faba bean,with its high protein yield and low nitrogen inputs,is a promising crop,but it suffers from low yield stability due to poor heat and drought adaptation.To improve yield performance,faba beans can be produced as synthetic cultivars,where multiple lines are advanced through open pollination resulting in offsprings,which are a mixture of F_(1)-hybrids and self-pollinated offsprings.This leads to an enhanced performance due to per se performance of the components and heterotic effects of F_(1)-hybrids.While distinct genetic pools have shown high heterotic effects in hybrid breeding programs,they have not been systematically established in faba bean breeding.To promote establishment of heterotic pools,we employed a cost-effective chain-crossing scheme accompanied with only 58 genome-wide KASP-markers and generated diverse genetically distinct pools within one generation.However,artificial crossing methods in faba bean result in low crossing efficiency and seed set.To overcome this,we introduced bumblebee-assisted intercrossing in greenhouse chambers,achieving an increased seed set and further enhancing genetic distance between gene pools.Genotyping was conducted with an Illumina 10K SNP-chip,which enabled the identification of F_(1)×F_(1) double-cross combinations and F2 self-pollinated offsprings from open pollinated offsprings with a custom pipeline.In contrast to standard crossing and recombinant inbred line(RIL)production in faba bean,which results in small families with limited recombination,the chain-crossing scheme and within-pool open pollination allows us to rapidly generate large and diverse base populations for future breeding,genetic studies and with that to increase genetic gain in faba bean.展开更多
The genus Beta encompasses economically important root crops such as sugar and table beet.A Beta diversity set including the wild relative B.vulgaris ssp.maritima was grown in the field,and a large phenotypic diversit...The genus Beta encompasses economically important root crops such as sugar and table beet.A Beta diversity set including the wild relative B.vulgaris ssp.maritima was grown in the field,and a large phenotypic diversity was observed.The genomes of 290 accessions were sequenced,and more than 10 million high-quality SNPs were employed to study genetic diversity.A genome-wide association study was performed,and marker-trait associations were found for nine phenotypic traits.The candidate gene within the M locus controlling monogermity on chromosome 4 was previously unknown.The most significant association for monogermity was identified at the end of chromosome 4.Within this region,a non-synonymous mutation within the zinc-finger domain of the WIP2 gene co-segregated with monogermity.This gene plays a regulatory role in AGL8/FUL in Arabidopsis.Intriguingly,commercial hybrids are in a heterozygous state at this position.Thus,the long-sought gene for monogermity was identified in this study.Red and yellow pigmentation due to betalain accumulation in shoots and roots is an important characteristic of table and leaf beets.The strongest associations were found upstream or downstream of two genes encoding Cytochrome P450 and anthocyanin MYB-like transcription factor proteins involved in betalain biosynthesis.Significant associations for Cercospora leaf spot resistance were identified on chromosomes 1,2,7,and 9.The associated regions harbor genes encoding proteins with leucinerich repeats and nucleotide binding sites whose homologs are major constituents of plant-pathogen defense.展开更多
In addition to the negative consequences of climate change,sucking pest complexes severely limited cotton yields in the recent past.Although the damage caused by bollworms was much reduced by utilizing Bt cotton,the e...In addition to the negative consequences of climate change,sucking pest complexes severely limited cotton yields in the recent past.Although the damage caused by bollworms was much reduced by utilizing Bt cotton,the emergence of sucking pests(such as aphids,thrips,and whiteflies)poses a serious threat to cotton production,as they reduce lint yield by 40%–60%finally.Additionally,these pests also caused yield losses by spreading viral diseases.Promoting innovative and thorough control methods is necessary to counter the threat posed by these sucking pests.Such initiatives necessitate a multifaceted strategy that combines next-generation breeding technology and pest management techniques to produce novel cotton cultivars that are resistant to sucking pests.The discovery of novel genes and regulatory factors linked to cotton’s resistance to sucking pests will be possible by the combination of next-generation breeding technologies and omics approaches and employing those tools on special resistant donors.Continuous research aimed at understanding the genetic basis of insect resistance and improving integrated pest management(IPM)techniques is crucial to the sustainability and resilience of cotton cropping systems.To this end,a sustainable and viable strategy to protect cotton fields from sucking pests is outlined.展开更多
Global warming is making plants more susceptible to heat stress.Hence,adjustments to crop production systems are required for global food security.Heat stress(HS)poses a threat to the quality of ecosystems and global ...Global warming is making plants more susceptible to heat stress.Hence,adjustments to crop production systems are required for global food security.Heat stress(HS)poses a threat to the quality of ecosystems and global food security due to its adverse effects on plant development.The degree to which HS affects physiological disruptions,physical harm,and biochemical changes at various growth stages directly correlates with its effects on physiological functions,plant growth,and crop production.One promising approach is soil modification using biochar,which enhances soil health and promotes the development of microbial communities,ultimately improving plant heat tolerance.Biochar enhances soil structure,improves moisture retention,and increases nutrient availability in hot weather,thereby promoting plant growth and enhancing crop yields.Additionally,biochar,with its porous structure and ability to provide a liming effect,increases the diversity and activity of soil microbes,thereby fostering advantageous symbiotic relationships.These microbial communities support nutrient cycling,root growth,and general soil health,strengthening biochar’s position as a long-term solution for climate-resilient farming.Earlier research concentrated on the connection between biochar and heat stress or microbial populations;however,this review uniquely combines all three elements,providing a fresh viewpoint on their interrelated functions in enhancing plant adaptability.Furthermore,this study demonstrates the potential of biochar as a sustainable component for improving soil and supporting crops that adapt to heat stress.It examines the processes underlying these interactions and provides recommendations for future research strategies.展开更多
The analysis of combining ability and heterosis is very important in enhancing the yield and oil quality of sunflowers under adverse conditions,and it reveals the potential of the parents and the mechanism of gene act...The analysis of combining ability and heterosis is very important in enhancing the yield and oil quality of sunflowers under adverse conditions,and it reveals the potential of the parents and the mechanism of gene action.In this study,twenty-one hybrids were developed by crossing seven cytoplasmic male sterile(CMS)lines with three restorer lines and evaluated for agronomic and quality traits.Highly significant general combining ability(GCA)and specific combining ability(SCA)effects were observed,confirming the role of both additive and non-additive gene actions.Among the tested crosses,A-42×R-86,A-92×R-86,and A-92×R-114 exhibited the greatest heterotic advantage,with seed yields exceeding 340 kg ha^(−1) over the better parent,oil contents above 19%,and 100-seed weights greater than 27 g.The hybrid A-92×R-114 was particularly notable for its elevated oleic acid level and balanced fatty acid profile,making it a strong candidate for premium oilseed production.In contrast,hybrids like A-20×R-39 exhibited moderate heterosis and less quality superiority.The oleic-to-linoleic acid ratio,a key determinant of oil stability,was strongly controlled by genetic factors.Oil content was largely influenced by additive effects,whereas yield heterosis was predominantly governed by non-additive effects.Overall,A-42×R-86 and A-92×R-114 emerged as the most promising hybrids,combining yield benefits with improved oil quality,and offering practical guidance for parental selection in sunflower breeding programs.展开更多
Plants produce many amino acid derivatives(AADs).Some have been used by humans as medicines and nutrients,but many also act as phytochemical signals in plant growth and stress tolerance.The fluctuating ecological envi...Plants produce many amino acid derivatives(AADs).Some have been used by humans as medicines and nutrients,but many also act as phytochemical signals in plant growth and stress tolerance.The fluctuating ecological environment poses a constant challenge to plant growth and development,and also presents significant obstacles to agricultural productivity.Plant AADs hold substantial potential for agricultural applications to increase plant resilience against diverse biological and environmental pressures.In this review,we present recent advances in elucidating the biological roles of plant AADs in plant growth and stress tolerance and outline strategies for discovering novel AADs and their regulatory networks in crops.The review aims to gain new insights into the functional properties of AADs in regulating plant growth and stress responses,which provides a valuable foundation for developing innovative AADbased strategies to improve crop performance and resilience facing the ever-changing environment in the future.展开更多
Light and nitrogen(N)are two critically environmental factors essential for plant survival,as they constitute the fundamental molecular framework of plant cells and significantly influence patterns of plant growth and...Light and nitrogen(N)are two critically environmental factors essential for plant survival,as they constitute the fundamental molecular framework of plant cells and significantly influence patterns of plant growth and development.Light is the driving force behind photosynthesis,a process that converts light energy into chemical energy stored as sugars.Additionally,light acts as a direct signal that can modulate plant morphogenesis and structural development.Nitrogen,as the most crucial mineral nutrient for plants,is a component of numerous biomolecules.It also functions as a signaling molecule,regulating plant growth and development.Moreover,light and nitrogen directly regulate the balance of carbon(C)and N within plants,affecting numerous biochemical reactions and various physiological processes.This review focuses on the interactions between light and nitrogen in physiological,metabolic,and molecular levels.We will also discuss the regulatory networks and mechanisms through which light and nitrogen influence C and N absorption and metabolism in plants.展开更多
Soil salinization affecting different crops is one of the serious threats to global food security.Soil salinity affects 20%and 33%of the total cultivated and irrigated agricultural lands,respectively,and has been repo...Soil salinization affecting different crops is one of the serious threats to global food security.Soil salinity affects 20%and 33%of the total cultivated and irrigated agricultural lands,respectively,and has been reported to caused a global crop production loss of 27.3 billion USD.The conventional approaches,such as using salt-tolerant varieties,saline soil scrapping,flushing,leaching,and adding supplements (e.g.,gypsum and lime),often fail to alleviate stress.In this context,developing diverse arrays of microbes enhancing crop productivity under saline soil conditions without harming soil health is necessary.Various advanced omics approaches have enabled gaining new insights into the structure and metabolic functions of plant-associated beneficial microbes.Various genera of salt-tolerating rhizobacteria ameliorating biotic and abiotic stresses have been isolated from different legumes,cereals,vegetables,and oil seeds under extreme alkaline and saline soil conditions.Rapid progress in rhizosphere microbiome research has revived the belief that plants may be more benefited from their association with interacting diverse microbial communities as compared with individual members in a community.In the last decade,several salt-tolerating plant growth-promoting rhizobacteria (PGPR) that improve crop production under salt stress have been exploited for the reclamation of saline agrosystems.This review highlights that the interaction of salt-tolerating microbes with plants improves crop productivity under salinity stress along with potential salt tolerance mechanisms involved and will open new avenues for capitalizing on cultivable diverse microbial communities to strengthen plant salt tolerance and,thus,to refine agricultural practices and production under saline conditions.展开更多
In the protocol of wheat transformation, to use elite wheat cultivars as exogenous gene recipients can speed up the process of commercial field applications of transgenic wheat. However, it is necessary to screen whea...In the protocol of wheat transformation, to use elite wheat cultivars as exogenous gene recipients can speed up the process of commercial field applications of transgenic wheat. However, it is necessary to screen wheat cultivars with good tissue culture response （TCR） continuously from plenty of elite wheat cultivars released for wheat transformation, and it is also important to find a plant regeneration system that is suitable for these cultivars. So, the TCR of mature and immature embryos of six wheat cultivars Chuannong 11 （CN11）, Chuannong12 （CN12）, Chuannong17 （CN17）, Chuannong18 （CN18）, Chuannong19 （CN19）, and Chuannong21 （CN21）, which possess superior agronomic traits, were investigated by using a good TCR wheat cultivar Bobwhite as control. The results indicated that only the immature and mature embryos of CN12, CN17, and CN18 exhibited good TCR compared with Bobwhite. No significant differences were observed between embryos of Bobwhite and of the three cultivars in TCR. Mature embryo-derived calli of CN12 were used as explants for transformation by particle bombardment of SAMDC gene. Seven transformants were obtained and the efficiency was 2.3%. This research supplies three new elite recipient cultivars for wheat transformation. The wheat plant regeneration system used in this research is different from those successful ones reported previously and it could be a reference for other wheat genotypes. Furthermore, Bobwhite and the three wheat cultivars were proved to be 1RS/1BL translocation, by methods of A-PAGE, C- banding, and genomic in situ hybridization （GISH）. These results imply that probably there is some relationship between 1RS/1BL translocation and TCR of wheat embryos. So this research gives us a hint that we should pay more attention to the 1RS/1BL translocations when we screen the wheat cultivars with good TCR and also that the mechanism of the effect of 1RS/ 1BL translocation on TCR is worthy of being investigated.展开更多
文摘The present work investigated the efficiency of leaf reflectance indices in the identification of Capsicum annuum L. var. annuum resistant to anthracnose in the fruit. Twenty-five F<sub>5:6</sub> families originating from contrasting parents were assessed;the parents were accession UENF 2285 (susceptible to anthracnose) and accession UENF 1381, a hot pepper resistant to anthracnose in the fruit. The experiment was carried out in an experimental field in Campos dos Goytacazes, Rio de Janeiro, Brazil, between May and October of 2021. The treatments were arranged in a randomized block design, with three replications and five plants per plot. Fifteen LRIs were estimated using a CI-710 portable mini leaf spectrometer. The assessments covered all plant growth after flowering, and a total of six assessments were performed at 15-days intervals, beginning at 35 and ending 120 days after flowering (DAFs). Analysis of variance in a split-plot scheme was performed, as were tests of mean groupings and principal components analysis (PCA). The best period for evaluating leaf reflectance indices in C. annuum var. annuum is 120 days after flowering. The leaf reflectance indices PRI, CNDVI and Ctr2 stood out as effective in distinguishing between resistant and susceptible genotypes.
基金funded by Uttar Banga Krishi Viswavidyalaya,IndiaBangladesh Wheat and Maize Research Institute,Dinajpur 5200,Bangladeshfunded by Taif University,Saudi Arabia,Project No.(TU-DSPP-2025-30).
文摘The present study investigated the genetic diversity of 24 germplasms of Polianthes tuberosa L.via 16 inter simple sequence repeat(ISSR)marker techniques.The research findings revealed that the ISSR markers presented higher levels of band reproducibility andweremore efficient at clustering germplasms.Among the 16markers examined in this study,12 had a complete polymorphism rate of 100%.Themolecular analysis revealed a PICranging from0.079 to 0.373,with amean value of 0.30,whereas the range of themarker index was from0.0001 to 0.409,with an average value of 0.03,and the primer resolving power ranged from 0.173 to 4.173,with a mean value of 2.02.The UPGMA clustering dendrogram indicated that all 24 germplasms were grouped into three main clusters.The study revealed a variable range of tree distances between 0.185 and 0.621,with the highest tree distance(0.621)detected between germplasms BR-24 and BR-1.Through these studies,the dissimilarity among the germplasms was evaluated,and diverse parents were identified for further crop improvement programs.
基金supported by SLU Grogrund(#SLU-LTV.2020.1.1.1-654)an Einar and Inga Nilsson Foundation grant.J.I.y.S.was supported by grant PID2021-123718OB-I00+4 种基金funded by MCIN/AEI/10.13039/501100011033by“ERDF A way of making Europe,”CEX2020-000999-S.R.R.V.supported by Novo Nordisk Fonden(0074727)SLU’s Centre for Biological ControlIn addition,J.I.y.S.and J.F.-G.were supported by the Beatriz Galindo Program BEAGAL 18/00115.
文摘Genomic selection,the application of genomic prediction(GP)models to select candidate individuals,has significantly advanced in the past two decades,effectively accelerating genetic gains in plant breeding.This article provides a holistic overview of key factors that have influenced GP in plant breeding during this period.We delved into the pivotal roles of training population size and genetic diversity,and their relationship with the breeding population,in determining GP accuracy.Special emphasis was placed on optimizing training population size.We explored its benefits and the associated diminishing returns beyond an optimum size.This was done while considering the balance between resource allocation and maximizing prediction accuracy through current optimization algorithms.The density and distribution of single-nucleotide polymorphisms,level of linkage disequilibrium,genetic complexity,trait heritability,statistical machine-learning methods,and non-additive effects are the other vital factors.Using wheat,maize,and potato as examples,we summarize the effect of these factors on the accuracy of GP for various traits.The search for high accuracy in GP—theoretically reaching one when using the Pearson’s correlation as a metric—is an active research area as yet far from optimal for various traits.We hypothesize that with ultra-high sizes of genotypic and phenotypic datasets,effective training population optimization methods and support from other omics approaches(transcriptomics,metabolomics and proteomics)coupled with deep-learning algorithms could overcome the boundaries of current limitations to achieve the highest possible prediction accuracy,making genomic selection an effective tool in plant breeding.
文摘Oenothera speciosa, belonging to thermophilous plant, cannot overwinter in Beijing. To enhance the overwintering rate of Oenothera speciosa, the seeds were treated through silico ion implantation (SII), with five various fluence ranges (1 × 109 - 1 × 1011 ions/cm2) of 40 MeV and four various fluence ranges (1 × 1010 - 5 × 1011 ions/cm2) of 35 MeV, respectively. M1 generations of various SII-treated Oenothera speciosa lines can overwinter, and the highest overwinter rate (41.3%) was observed in Oenothera speciosa lines treated with 35 MeV and fluence 5 × 1010 ions/cm2. M2 and M3 generations of all treated lines were able to overwinter smoothly. The results indicated that SII treatment can enhance the cold-resistance of Oenothera speciosa heritably. Furthermore, physiological indexes including relative electrical conductivity, MDA contents and proline contents of SII-treated Oenothera speciosa pot seedlings were detected after low temperature stress. The results revealed that relative electrical conductivities and MDA contents of M1, M2 and M3 generations of SII-treated Oenothera speciosa plants were lower than that of control, whereas the proline contents were higher than control in the LJ°C cold stress. Taken together, the cold resistance of SII-treated Oenothera speciosa plants was improved, which made it possible to be used as a perennial flower in landscaping in Beijing.
基金funded by Department of Science&Technology and Biotechnology,Govt.of West Bengal,India[Sanction No.565(Sanc.)/STBT-13015/15/11/2021-ST SEC]。
文摘Gerbera,a popular commercial cut flower with vibrant and striking colors has gained immense popularity in the floriculture industry.They are widely cultivated in various regions,making them available throughout the year.As a better alternative to conventional propagation methods(via seeds and rhizomes),plant tissue culture serves as way to avail large-scale,uniform,disease-free plantlets for commercial cultivation as well as to develop novel genotypes.In addition,it ensures production of healthy plantlets throughout the year in limited space.Based on the plant tissue culture techniques,the in vitro polyploidization,mutagenesis,and genetic transformation pave a path for creation of variation and eventually enhancing the ornamental traits to address the consumers’preferences and also facilitates in developing stress tolerant lines thereby minimizing the losses during cultivation,maintaining the quality of the flowers.This comprehensive review article presents an overview of the recent advancements on genetic improvement of gerbera via various cutting-edge plant tissue culture-based tools and techniques that contribute in enhancing the quality and efficiency of gerbera cultivation,meeting the demands of the floriculture industry while addressing the challenges of changing environment and resource limitations.
基金The authors extend their gratitude to the Deanship of Scientific Research(DSR),King Faisal University,Saudi Arabia,for funding the publication of this work(Project number:KFU250560).
文摘A steady rise in the overall population is creating an overburden on crops due to their global demand.On the other hand,given the current climate change and population growth,agricultural practices established during the Green Revolution are no longer viable.Consequently,innovative practices are the prerequisite of the time struggle with the rising global food demand.The potential of nanotechnology to reduce the phytotoxic effects of these ecological restrictions has shown significant promise.Nanoparticles(NPs)typically enhance plant resilience to stressors by fortifying the physical barrier,optimizing photosynthesis,stimulating enzymatic activity for defense,elevating the concentration of stress-resistant compounds,and activating the expression of genes associated with defense mechanisms.In this review,we thoroughly cover the uptake and translocations of NPs crops and their potential valuable functions in enhancing plant growth and development at different growth stages.Additionally,we addressed how NPs improve plant resistance to biotic and abiotic stress.Generally,this review presents a thorough understanding of the significance of NPs in plants and their prospective value for plant antioxidant and crop development.
文摘Molecular tools have drawn the attention ofmodern plant breeders for its great precision and superiority.As the global population is increasing gradually,food production should be enhanced to feed the growing population.Therefore,precise and fast breeding tools are becoming obvious.Moreover,climate change has become a critical issue in crop improvement.Advanced breeding methods are vital to combat the impact of climate change,including biotic and abiotic stresses.Major molecular techniques,such as‘CRISPR-Cas’mediated‘genome editing’,‘marker-assisted selection(MAS)’,‘whole genome sequencing’,‘RNAi’,transgenic approach,‘high-throughput phenotyping(HTP)’,mutation breeding,have been proven superior over traditional breeding in terms of precision,efficiency,and speed in developing stress-resistant improved varieties.This review explores the potential and superiority ofmolecular breeding methods and highlights the gaps(time,cost,efficiency,etc.)in traditional breeding methods,where modern breeding programs,asmentioned,are effective.Furthermore,this reviewwill focus on the necessity of keymodern plant breeding techniques as a foundation for sustainable farming practices to address emerging environmental challenges,ensure food security,and improve the yield and quality of crops.
基金funded by Bangladesh Academy of Sciences(BAS),and Fund ID:BAS-USDA SAU CR02.
文摘Indian mustard is recognized as a resilient and economically important oilseed crop.However,its potential remains untapped due to the limited availability of short-duration,high-yielding varieties capable of out-competing other rabi crops.Considering this notion,we have evaluated twenty-one F_(2) and six BC1F1 populations derived from seven diversified parents of Brassica juncea following a Randomized Complete Block Design at Sher-e-Bangla Agricultural University.Based on key agronomic traits,the genetic components,heterosis,inbreeding depression,and gene action were studied to select early maturing and high-yielding populations.The percentage of heterosis was manifested in various cross-combinations,including P4×P6(91.45%for yield per plant)and P5×P6(28.52%for thousand seed weight),emerging as promising candidates for increasing productivity while managing negative inbreeding effects.Conversely,significant inbreeding depression was noted in traits like days to siliquae maturity and yield,particularly in crosses,P1×P2(6.29%)and P3×P5(21.74%),underscoring the need for careful selection in breeding programs to mitigate these effects.Variance analysis indicated that both additive and non-additive genetic interactions play a pivotal role in the inheritance patterns of the traits of interest.Among the six backcrosses,one promising line was(P5×P6)×P5,demonstrating early maturity(107.00 DAS)with improved seed yield(12.47 g).This combination exhibited the potential for enhancing the adaptability and productivity by maintaining the maturity index and accelerating yield.Furthermore,significant phenotypic variation across yield-contributing traits was notable,whereas thousand seed weight and yield per plant showed high broad-sense and narrow-sense of heritability.Besides,positive correlations between seed yield and its attributing traits were noted,suggesting potential avenues for selection breeding.Collectively,the ob-tained findings enhance the understanding of genetic mechanisms underlying heterosis and inbreeding depres-sion in B.juncea,providing insights and effective strategies for developing superior cultivars with optimized agronomic traits.
文摘Phytic acid(PA), or myo-inositol 1,2,3,4,5,6-hexakisphosphate, is the main storage form of phosphorus(P) in seeds, accounting for 65% to 85% of their total P content. The negative charge of PA attracts metal cations, forming insoluble salts called phytates. These phytates, contain six negatively charged ions, can bind divalent cations such as Fe^(2+), Zn^(2+), Mg^(2+), and Ca^(2+), preventing their absorption in monogastric animals. To overcome P deficiency in non-ruminants, phytase is usually given as a supplement, which then results in excess P excretion, leading to environmental problems such as eutrophication. Improved fertilizer management, food processing techniques, and the development of low-PA crops through plant breeding are envisioned as effective ways to improve P-utilization and lessen the environmental impact while minimizing the effect of PA. A better understanding of the molecular and physiological basis of PA biosynthesis, grain PA distribution, the effects of genetic and environmental factors on PA accumulation, and methods to increase micronutrient bioavailability by lowering the effects of PA is essential for developing low-PA crops.
文摘The ability of plants to tolerate cold is a complex process.When temperatures drop or freeze,plant tissues can develop ice,which dehydrates the cells.However,plants can protect themselves by preventing ice formation.This intricate response to cold stress is regulated by hormones,photoperiod,light,and various factors,in addition to genetic influences.In autumn,plants undergo morphological,physiological,biochemical,and molecular changes to prepare for the low temperatures of winter.Understanding cellular stress responses is crucial for genetic manipulation aimed at enhancing cold resistance.Early autumn frosts or late spring chills can cause significant damage to plants,making it essential to adapt in autumn to survive winter conditions.While the general process of acclimatization is similar across many plant species,variations exist depending on the specific type of plant and regional conditions.Different plant organs exhibit varying degrees of damage from cold stress,and by applying agricultural principles,potential damage can be largely controlled.Timely reinforcement and stress prevention can minimize cold-related damage.Research has shown that in temperate climates,low temperatures restrict plant growth and yield.However,the intricate structural systems involved remain poorly understood.Over the past decade,studies have focused on the molecular mechanisms that enable plants to adapt to and resist cold stress.The gene signaling system is believed to play a crucial role in cold adaptation,and researchers have prioritized this area in their investigations.This study critically examines plant responses to cold stress through physiological adaptations,including calcium signaling dynamics,membrane lipid modifications,and adjustments in antioxidant systems.These mechanisms activate downstream gene expression and molecular functions,leading to key resistance strategies.Additionally,we explore the regulatory roles of endogenous phytohormones and secondary metabolites in cold stress responses.This review aims to enhance our foundational understanding of the mechanisms behind plant cold adaptation.
基金funded by grant 469336000 to Rod J.Snowdon from the German Research Society(DFG)for the International Research Training Group 2843“Accelerating crop Genetic Gain”.
文摘Faba bean,with its high protein yield and low nitrogen inputs,is a promising crop,but it suffers from low yield stability due to poor heat and drought adaptation.To improve yield performance,faba beans can be produced as synthetic cultivars,where multiple lines are advanced through open pollination resulting in offsprings,which are a mixture of F_(1)-hybrids and self-pollinated offsprings.This leads to an enhanced performance due to per se performance of the components and heterotic effects of F_(1)-hybrids.While distinct genetic pools have shown high heterotic effects in hybrid breeding programs,they have not been systematically established in faba bean breeding.To promote establishment of heterotic pools,we employed a cost-effective chain-crossing scheme accompanied with only 58 genome-wide KASP-markers and generated diverse genetically distinct pools within one generation.However,artificial crossing methods in faba bean result in low crossing efficiency and seed set.To overcome this,we introduced bumblebee-assisted intercrossing in greenhouse chambers,achieving an increased seed set and further enhancing genetic distance between gene pools.Genotyping was conducted with an Illumina 10K SNP-chip,which enabled the identification of F_(1)×F_(1) double-cross combinations and F2 self-pollinated offsprings from open pollinated offsprings with a custom pipeline.In contrast to standard crossing and recombinant inbred line(RIL)production in faba bean,which results in small families with limited recombination,the chain-crossing scheme and within-pool open pollination allows us to rapidly generate large and diverse base populations for future breeding,genetic studies and with that to increase genetic gain in faba bean.
基金the financial support from the German Research Foundation(Deutsche Forschungsgemeinschaft,DFG)—Project Number 400993799(Project 2 within the Research Training Group 2501 Translational Evolutionary Research,https://gepris.dfg.de/gepris/projekt/400993799)supported by the BMBF-funded de.NBI Cloud,part of the German Network for Bioinformatics(de.NBI)。
文摘The genus Beta encompasses economically important root crops such as sugar and table beet.A Beta diversity set including the wild relative B.vulgaris ssp.maritima was grown in the field,and a large phenotypic diversity was observed.The genomes of 290 accessions were sequenced,and more than 10 million high-quality SNPs were employed to study genetic diversity.A genome-wide association study was performed,and marker-trait associations were found for nine phenotypic traits.The candidate gene within the M locus controlling monogermity on chromosome 4 was previously unknown.The most significant association for monogermity was identified at the end of chromosome 4.Within this region,a non-synonymous mutation within the zinc-finger domain of the WIP2 gene co-segregated with monogermity.This gene plays a regulatory role in AGL8/FUL in Arabidopsis.Intriguingly,commercial hybrids are in a heterozygous state at this position.Thus,the long-sought gene for monogermity was identified in this study.Red and yellow pigmentation due to betalain accumulation in shoots and roots is an important characteristic of table and leaf beets.The strongest associations were found upstream or downstream of two genes encoding Cytochrome P450 and anthocyanin MYB-like transcription factor proteins involved in betalain biosynthesis.Significant associations for Cercospora leaf spot resistance were identified on chromosomes 1,2,7,and 9.The associated regions harbor genes encoding proteins with leucinerich repeats and nucleotide binding sites whose homologs are major constituents of plant-pathogen defense.
基金M/s.RASI Seeds Pvt.Ltd.,Attur,Tamil Nadu,India for their generous financial assistance in setting up a MAS study in cotton for genetic improvement of sucking pest resistance.
文摘In addition to the negative consequences of climate change,sucking pest complexes severely limited cotton yields in the recent past.Although the damage caused by bollworms was much reduced by utilizing Bt cotton,the emergence of sucking pests(such as aphids,thrips,and whiteflies)poses a serious threat to cotton production,as they reduce lint yield by 40%–60%finally.Additionally,these pests also caused yield losses by spreading viral diseases.Promoting innovative and thorough control methods is necessary to counter the threat posed by these sucking pests.Such initiatives necessitate a multifaceted strategy that combines next-generation breeding technology and pest management techniques to produce novel cotton cultivars that are resistant to sucking pests.The discovery of novel genes and regulatory factors linked to cotton’s resistance to sucking pests will be possible by the combination of next-generation breeding technologies and omics approaches and employing those tools on special resistant donors.Continuous research aimed at understanding the genetic basis of insect resistance and improving integrated pest management(IPM)techniques is crucial to the sustainability and resilience of cotton cropping systems.To this end,a sustainable and viable strategy to protect cotton fields from sucking pests is outlined.
文摘Global warming is making plants more susceptible to heat stress.Hence,adjustments to crop production systems are required for global food security.Heat stress(HS)poses a threat to the quality of ecosystems and global food security due to its adverse effects on plant development.The degree to which HS affects physiological disruptions,physical harm,and biochemical changes at various growth stages directly correlates with its effects on physiological functions,plant growth,and crop production.One promising approach is soil modification using biochar,which enhances soil health and promotes the development of microbial communities,ultimately improving plant heat tolerance.Biochar enhances soil structure,improves moisture retention,and increases nutrient availability in hot weather,thereby promoting plant growth and enhancing crop yields.Additionally,biochar,with its porous structure and ability to provide a liming effect,increases the diversity and activity of soil microbes,thereby fostering advantageous symbiotic relationships.These microbial communities support nutrient cycling,root growth,and general soil health,strengthening biochar’s position as a long-term solution for climate-resilient farming.Earlier research concentrated on the connection between biochar and heat stress or microbial populations;however,this review uniquely combines all three elements,providing a fresh viewpoint on their interrelated functions in enhancing plant adaptability.Furthermore,this study demonstrates the potential of biochar as a sustainable component for improving soil and supporting crops that adapt to heat stress.It examines the processes underlying these interactions and provides recommendations for future research strategies.
文摘The analysis of combining ability and heterosis is very important in enhancing the yield and oil quality of sunflowers under adverse conditions,and it reveals the potential of the parents and the mechanism of gene action.In this study,twenty-one hybrids were developed by crossing seven cytoplasmic male sterile(CMS)lines with three restorer lines and evaluated for agronomic and quality traits.Highly significant general combining ability(GCA)and specific combining ability(SCA)effects were observed,confirming the role of both additive and non-additive gene actions.Among the tested crosses,A-42×R-86,A-92×R-86,and A-92×R-114 exhibited the greatest heterotic advantage,with seed yields exceeding 340 kg ha^(−1) over the better parent,oil contents above 19%,and 100-seed weights greater than 27 g.The hybrid A-92×R-114 was particularly notable for its elevated oleic acid level and balanced fatty acid profile,making it a strong candidate for premium oilseed production.In contrast,hybrids like A-20×R-39 exhibited moderate heterosis and less quality superiority.The oleic-to-linoleic acid ratio,a key determinant of oil stability,was strongly controlled by genetic factors.Oil content was largely influenced by additive effects,whereas yield heterosis was predominantly governed by non-additive effects.Overall,A-42×R-86 and A-92×R-114 emerged as the most promising hybrids,combining yield benefits with improved oil quality,and offering practical guidance for parental selection in sunflower breeding programs.
基金funded by National Natural Science Foundation of China(32472034)Guangdong Province Natural Science Foundation(2022A1515010707)。
文摘Plants produce many amino acid derivatives(AADs).Some have been used by humans as medicines and nutrients,but many also act as phytochemical signals in plant growth and stress tolerance.The fluctuating ecological environment poses a constant challenge to plant growth and development,and also presents significant obstacles to agricultural productivity.Plant AADs hold substantial potential for agricultural applications to increase plant resilience against diverse biological and environmental pressures.In this review,we present recent advances in elucidating the biological roles of plant AADs in plant growth and stress tolerance and outline strategies for discovering novel AADs and their regulatory networks in crops.The review aims to gain new insights into the functional properties of AADs in regulating plant growth and stress responses,which provides a valuable foundation for developing innovative AADbased strategies to improve crop performance and resilience facing the ever-changing environment in the future.
基金supported by grants from Hainan Seed Laboratory(B21HJ0003)the National Natural Science Foundation of China(U23A20185)the Hainan Excellent Talent Team。
文摘Light and nitrogen(N)are two critically environmental factors essential for plant survival,as they constitute the fundamental molecular framework of plant cells and significantly influence patterns of plant growth and development.Light is the driving force behind photosynthesis,a process that converts light energy into chemical energy stored as sugars.Additionally,light acts as a direct signal that can modulate plant morphogenesis and structural development.Nitrogen,as the most crucial mineral nutrient for plants,is a component of numerous biomolecules.It also functions as a signaling molecule,regulating plant growth and development.Moreover,light and nitrogen directly regulate the balance of carbon(C)and N within plants,affecting numerous biochemical reactions and various physiological processes.This review focuses on the interactions between light and nitrogen in physiological,metabolic,and molecular levels.We will also discuss the regulatory networks and mechanisms through which light and nitrogen influence C and N absorption and metabolism in plants.
文摘Soil salinization affecting different crops is one of the serious threats to global food security.Soil salinity affects 20%and 33%of the total cultivated and irrigated agricultural lands,respectively,and has been reported to caused a global crop production loss of 27.3 billion USD.The conventional approaches,such as using salt-tolerant varieties,saline soil scrapping,flushing,leaching,and adding supplements (e.g.,gypsum and lime),often fail to alleviate stress.In this context,developing diverse arrays of microbes enhancing crop productivity under saline soil conditions without harming soil health is necessary.Various advanced omics approaches have enabled gaining new insights into the structure and metabolic functions of plant-associated beneficial microbes.Various genera of salt-tolerating rhizobacteria ameliorating biotic and abiotic stresses have been isolated from different legumes,cereals,vegetables,and oil seeds under extreme alkaline and saline soil conditions.Rapid progress in rhizosphere microbiome research has revived the belief that plants may be more benefited from their association with interacting diverse microbial communities as compared with individual members in a community.In the last decade,several salt-tolerating plant growth-promoting rhizobacteria (PGPR) that improve crop production under salt stress have been exploited for the reclamation of saline agrosystems.This review highlights that the interaction of salt-tolerating microbes with plants improves crop productivity under salinity stress along with potential salt tolerance mechanisms involved and will open new avenues for capitalizing on cultivable diverse microbial communities to strengthen plant salt tolerance and,thus,to refine agricultural practices and production under saline conditions.
基金We thank Dr.Yang Zujun,Zhang Huaiyu,Yan Benju,Tan Feiquan,and Zhou Jianpin for their helpful comments in improving the manuscript.We also thank Cheng Jing for providing wheat cv.Bobwhite.This work was supported by 948 Project of Ministry of Agriculture,China(246)the National Natural Science Foundation of China(30170579).
文摘In the protocol of wheat transformation, to use elite wheat cultivars as exogenous gene recipients can speed up the process of commercial field applications of transgenic wheat. However, it is necessary to screen wheat cultivars with good tissue culture response （TCR） continuously from plenty of elite wheat cultivars released for wheat transformation, and it is also important to find a plant regeneration system that is suitable for these cultivars. So, the TCR of mature and immature embryos of six wheat cultivars Chuannong 11 （CN11）, Chuannong12 （CN12）, Chuannong17 （CN17）, Chuannong18 （CN18）, Chuannong19 （CN19）, and Chuannong21 （CN21）, which possess superior agronomic traits, were investigated by using a good TCR wheat cultivar Bobwhite as control. The results indicated that only the immature and mature embryos of CN12, CN17, and CN18 exhibited good TCR compared with Bobwhite. No significant differences were observed between embryos of Bobwhite and of the three cultivars in TCR. Mature embryo-derived calli of CN12 were used as explants for transformation by particle bombardment of SAMDC gene. Seven transformants were obtained and the efficiency was 2.3%. This research supplies three new elite recipient cultivars for wheat transformation. The wheat plant regeneration system used in this research is different from those successful ones reported previously and it could be a reference for other wheat genotypes. Furthermore, Bobwhite and the three wheat cultivars were proved to be 1RS/1BL translocation, by methods of A-PAGE, C- banding, and genomic in situ hybridization （GISH）. These results imply that probably there is some relationship between 1RS/1BL translocation and TCR of wheat embryos. So this research gives us a hint that we should pay more attention to the 1RS/1BL translocations when we screen the wheat cultivars with good TCR and also that the mechanism of the effect of 1RS/ 1BL translocation on TCR is worthy of being investigated.
