Background The alexithymia trait is of high clinical interest.The Perth Alexithymia Questionnaire(PAQ)was recently developed to enable detailed facet-level and valence-specific assessments of alexithymia.Aims In this ...Background The alexithymia trait is of high clinical interest.The Perth Alexithymia Questionnaire(PAQ)was recently developed to enable detailed facet-level and valence-specific assessments of alexithymia.Aims In this paper,we introduce the first Chinese version of the PAQ and examine its psychometric properties and clinical applications.Methods In Study 1,the PAQ was administered to 990 Chinese participants.We examined its factor structure,internal consistency,test-retest reliability,as well as convergent,concurrent and discriminant validity.In Study 2,four groups,including a major depressive disorder(MDD)group(n=50),a matched healthy control group for MDD(n=50),a subclinical depression group(n=50)and a matched healthy control group for subclinical depression(n=50),were recruited.Group comparisons were conducted to assess the clinical relevance of the PAQ.Results In Study 1,the intended five-factor structure of the PAQ was found to fit the data well.The PAQ showed good internal consistency and test-retest reliability,as well as good convergent,concurrent and discriminant validity.In Study 2,the PAQ was able to successfully distinguish the MDD group and the subclinical depression group from their matched healthy controls.Conclusions The Chinese version of the PAQ is a valid and reliable instrument for comprehensively assessing alexithymia in the general population and adults with clinical/subclinical depression.展开更多
Urban agriculture has been increasingly popular as a form of modern agriculture in urban settings. It includes community gardens, fruit orchards, home gardens, veggie patches, public open spaces, reserves, urban fores...Urban agriculture has been increasingly popular as a form of modern agriculture in urban settings. It includes community gardens, fruit orchards, home gardens, veggie patches, public open spaces, reserves, urban forest, and recreational landscaping. However, irrigation using urban water supply has been identified as a major constraints for the development of urban agriculture. This study presents a sustainable water management trial at Butler, a northern sub-urban development in Perth, Western Australia, for urban irrigation. The trial system consists of a number of water saving features including untreated fit-for-purpose groundwater supplied via a third pipe network, drip irrigation, local weather station, soil moisture sensors connected with a local weather station, night time irrigation, soil enhancement with conditioning and mulching, and use of native plants and vegetation. The trial outcome was compared against controlled areas in terms of irrigation efficiency and sustainable water management for urban agriculture. The study demonstrated that a fit-for-purpose irrigation along with water sensitive land management could be a sustainable alternative for urban agriculture that would achieve a significant water saving and irrigation efficiency at urban settings. However, quality of untreated groundwater can be an issue while utilizing it for irrigation, but the research has shown that it can be managed with innovative irrigation techniques. This indicates that the fit-for-purpose irrigation system with water sensitive land management practices would be highly supportive in sustainable development of urban agriculture, vegetation and recreational landscaping.展开更多
Tight gas sands in Whicher Range Field of Perth Basin show large heterogeneity in reservoir characteristics and production behavior related to depositional and diagenetic features. Diagenetic events (compaction and ce...Tight gas sands in Whicher Range Field of Perth Basin show large heterogeneity in reservoir characteristics and production behavior related to depositional and diagenetic features. Diagenetic events (compaction and cementation) have severely affected the pore system. In order to investigate the petrophysical characteristics, reservoir sandstone facies were correlated with core porosity and permeability and their equivalent well log responses to describe hydraulic flow units and electrofacies, respectively. Thus, very tight, tight, and sub-tight sands were differentiated. To reveal the relationship between pore system properties and depositional and diagenetic characteristics in each sand type, reservoir rock types were extracted. The identified reservoir rock types are in fact a reflection of internal reservoir heterogeneity related to pore system properties. All reservoir rock types are characterized by a compacted fabric and cemented framework. But distribution and dominance of diagenetic products in each of them depend on primary depositional composition and texture. The results show that reservoir rock typing based on three aspects of reservoir sandstones (depositional properties, diagenetic features and petrophysical characteristics) is a suitable technique for depiction of reservoir heterogeneity, recognition of reservoir units and identifying factors controlling reservoir quality of tight sandstones. This methodology can be used for the other tight reservoirs.展开更多
Background:Tandem gene repeats naturally occur as important genomic features and determine many traits in living organisms,like human diseases and microbial productivities of target bioproducts.Methods:Here,we develop...Background:Tandem gene repeats naturally occur as important genomic features and determine many traits in living organisms,like human diseases and microbial productivities of target bioproducts.Methods:Here,we developed a bacterial type-II toxin-antitoxin-mediated method to manipulate genomic integration of tandem gene repeats in Saccharomyces cerevisiae and further visualised the evolutionary trajectories of gene repeats.We designed a tri-vector system to introduce toxin-antitoxin-driven gene amplification modules.Results:This system delivered multi-copy gene integration in the form of tandem gene repeats spontaneously and independently from toxin-antitoxin-mediated selection.Inducing the toxin(RelE)expressing via a copper(II)-inducible CUP1 promoter successfully drove the in-situ gene amplification of the antitoxin(RelB)module,resulting in~40 copies of a green fluorescence reporter gene per copy of genome.Copy-number changes,copy-number increase and copy-number decrease,and stable maintenance were visualised using the green fluorescence protein and blue chromoprotein AeBlue as reporters.Copy-number increases happened spontaneously and independent on a selection pressure.Increased copy number was quickly enriched through toxin-antitoxin-mediated selection.Conclusion:In summary,the bacterial toxin-antitoxin systems provide a flexible mechanism to manipulate gene copy number in eukaryotic cells and can be exploited for synthetic biology and metabolic engineering applications.展开更多
目的:系统梳理与荟萃分析探究等长训练改善成年人静息血压的临床功效和影响因素。方法:检索PubMed、Web of Science、中国知网、维普和万方数据库自建库以来至2024年12月26日有关等长训练对成年人静息血压长期影响的随机对照试验。基于R...目的:系统梳理与荟萃分析探究等长训练改善成年人静息血压的临床功效和影响因素。方法:检索PubMed、Web of Science、中国知网、维普和万方数据库自建库以来至2024年12月26日有关等长训练对成年人静息血压长期影响的随机对照试验。基于R 4.3.3软件中的随机效应模型合并效应量,并执行亚组、回归分析和敏感性分析探索异质性来源和影响因素。结果:纳入37篇文献,共计1510例受试者,年龄为21~75岁。荟萃分析结果显示,相比于非运动干预对照,等长训练显著改善静息收缩压(Hedge's g=-0.79;95%CI:-0.98~-0.60;P<0.01)、静息舒张压(Hedge's g=-0.53;95%CI:-0.68~-0.38;P<0.01)和静息平均动脉压(Hedge's g=-0.82;95%CI:-1.02~-0.63;P<0.01)。亚组分析结果显示,在降低静息收缩压方面,等长训练对正常高值血压人群的降压效果相较于正常血压人群和高血压人群更显著;等长训练方式中,靠墙静蹲改善静息血压的效果最佳,且相较于等长握力有显著差异;等长训练对未用药人群的降压效果比用药人群更显著。等长训练降低非老年人群静息收缩压和静息舒张压的效果比老年人群更显著。回归分析结果显示,等长训练降低静息舒张压的效果受到年龄和间隔时长的影响;随着运动强度增加,等长训练降低静息平均动脉压的效果显著提升。结论:等长训练可有效降低成年人静息收缩压、静息舒张压和静息平均动脉压,其调控血压的效果呈现人群异质性,且受到年龄、间隔时长和运动强度的影响。靠墙静蹲是现有等长训练研究中降压效果最显著的干预方式,加之无不良反应报告且无需设备支持,适用于家庭和办公场所,可作为改善我国高血压现状长期有效的运动干预策略。展开更多
基金This work was supported by a grant from the National Natural Science Foundation of China(32200906)STI 2030-Major Projects(2021ZD0201705)+2 种基金Major Project of Philosophy and Social Science Research of the Ministry of Education of China(22JZD044)Research Project of Shanghai Science and Technology Commission(20dz2260300)Fundamental Research Funds for the Central Universities and Starting Research Fund from Hangzhou Normal University.
文摘Background The alexithymia trait is of high clinical interest.The Perth Alexithymia Questionnaire(PAQ)was recently developed to enable detailed facet-level and valence-specific assessments of alexithymia.Aims In this paper,we introduce the first Chinese version of the PAQ and examine its psychometric properties and clinical applications.Methods In Study 1,the PAQ was administered to 990 Chinese participants.We examined its factor structure,internal consistency,test-retest reliability,as well as convergent,concurrent and discriminant validity.In Study 2,four groups,including a major depressive disorder(MDD)group(n=50),a matched healthy control group for MDD(n=50),a subclinical depression group(n=50)and a matched healthy control group for subclinical depression(n=50),were recruited.Group comparisons were conducted to assess the clinical relevance of the PAQ.Results In Study 1,the intended five-factor structure of the PAQ was found to fit the data well.The PAQ showed good internal consistency and test-retest reliability,as well as good convergent,concurrent and discriminant validity.In Study 2,the PAQ was able to successfully distinguish the MDD group and the subclinical depression group from their matched healthy controls.Conclusions The Chinese version of the PAQ is a valid and reliable instrument for comprehensively assessing alexithymia in the general population and adults with clinical/subclinical depression.
文摘Urban agriculture has been increasingly popular as a form of modern agriculture in urban settings. It includes community gardens, fruit orchards, home gardens, veggie patches, public open spaces, reserves, urban forest, and recreational landscaping. However, irrigation using urban water supply has been identified as a major constraints for the development of urban agriculture. This study presents a sustainable water management trial at Butler, a northern sub-urban development in Perth, Western Australia, for urban irrigation. The trial system consists of a number of water saving features including untreated fit-for-purpose groundwater supplied via a third pipe network, drip irrigation, local weather station, soil moisture sensors connected with a local weather station, night time irrigation, soil enhancement with conditioning and mulching, and use of native plants and vegetation. The trial outcome was compared against controlled areas in terms of irrigation efficiency and sustainable water management for urban agriculture. The study demonstrated that a fit-for-purpose irrigation along with water sensitive land management could be a sustainable alternative for urban agriculture that would achieve a significant water saving and irrigation efficiency at urban settings. However, quality of untreated groundwater can be an issue while utilizing it for irrigation, but the research has shown that it can be managed with innovative irrigation techniques. This indicates that the fit-for-purpose irrigation system with water sensitive land management practices would be highly supportive in sustainable development of urban agriculture, vegetation and recreational landscaping.
文摘Tight gas sands in Whicher Range Field of Perth Basin show large heterogeneity in reservoir characteristics and production behavior related to depositional and diagenetic features. Diagenetic events (compaction and cementation) have severely affected the pore system. In order to investigate the petrophysical characteristics, reservoir sandstone facies were correlated with core porosity and permeability and their equivalent well log responses to describe hydraulic flow units and electrofacies, respectively. Thus, very tight, tight, and sub-tight sands were differentiated. To reveal the relationship between pore system properties and depositional and diagenetic characteristics in each sand type, reservoir rock types were extracted. The identified reservoir rock types are in fact a reflection of internal reservoir heterogeneity related to pore system properties. All reservoir rock types are characterized by a compacted fabric and cemented framework. But distribution and dominance of diagenetic products in each of them depend on primary depositional composition and texture. The results show that reservoir rock typing based on three aspects of reservoir sandstones (depositional properties, diagenetic features and petrophysical characteristics) is a suitable technique for depiction of reservoir heterogeneity, recognition of reservoir units and identifying factors controlling reservoir quality of tight sandstones. This methodology can be used for the other tight reservoirs.
基金supported partially by the Australian Government through the Australian Research Council Centres of Excellence funding scheme(project CE200100029)。
文摘Background:Tandem gene repeats naturally occur as important genomic features and determine many traits in living organisms,like human diseases and microbial productivities of target bioproducts.Methods:Here,we developed a bacterial type-II toxin-antitoxin-mediated method to manipulate genomic integration of tandem gene repeats in Saccharomyces cerevisiae and further visualised the evolutionary trajectories of gene repeats.We designed a tri-vector system to introduce toxin-antitoxin-driven gene amplification modules.Results:This system delivered multi-copy gene integration in the form of tandem gene repeats spontaneously and independently from toxin-antitoxin-mediated selection.Inducing the toxin(RelE)expressing via a copper(II)-inducible CUP1 promoter successfully drove the in-situ gene amplification of the antitoxin(RelB)module,resulting in~40 copies of a green fluorescence reporter gene per copy of genome.Copy-number changes,copy-number increase and copy-number decrease,and stable maintenance were visualised using the green fluorescence protein and blue chromoprotein AeBlue as reporters.Copy-number increases happened spontaneously and independent on a selection pressure.Increased copy number was quickly enriched through toxin-antitoxin-mediated selection.Conclusion:In summary,the bacterial toxin-antitoxin systems provide a flexible mechanism to manipulate gene copy number in eukaryotic cells and can be exploited for synthetic biology and metabolic engineering applications.
