Objective:To determine the prevalence of tick-borne pathogens with a particular focus on Rickettsia spp.in ticks collected from cattle in Gauteng and Limpopo Provinces,South Africa.Methods:A total of 200 ticks were co...Objective:To determine the prevalence of tick-borne pathogens with a particular focus on Rickettsia spp.in ticks collected from cattle in Gauteng and Limpopo Provinces,South Africa.Methods:A total of 200 ticks were collected from cattle within the Madala livestock,Pretoria,Gauteng Province and in Mankweng Township,Polokwane,Limpopo Province in 2019.The ticks were morphologically identified and processed individually for a total genomic DNA extraction.Specific primers targetting ompA,ompB,and the 17KDa genes were used for a molecular screening and delineation of Rickettsia from the extracted genetic materials using polymerase chain reaction(PCR)technique.PCR amplicons of positive samples were sequenced bidirectionally using the Sanger sequencing method.Sequences generated were processed and analysed using appropriate bioinformatics software.Results:The ticks were morphologically identified as Amblyomma spp.PCR profiling of the genomic DNA samples revealed the presence of the Rickettsia pathogen in 42(21%)of the ticks collected from both Provinces.Out of the genes profiled,14(7%)were positive for 17KDa,42(21%)for ompA and 32(16%)were positive for ompB genes respectively.The nucleotide blast of the sequenced genomes showed high similarity,as high as 100% with other reference Rickettsia(R.)africae in the GenBank.The phylogenetic analysis of the sequences further validated them as R.africae with their characteristic clustering pattern with related reference sequences.Conclusions:There is an abundance of R.africae in Amblyomma ticks collected from cattle in the study areas.This has serious public health implications as individuals who accidentally get infested with the ticks could acquire R.africae.Hence,adequate precautions in terms of sensitization of farmers about the risk and mass mobilization drive to control the vectors in the areas are highly recommended to safeguard public health.展开更多
Six months after the United Nations General Assembly 2nd high-level meeting in 26 September 2024 to review the progress made at all levels to tackle antimicrobial resistance(AMR)and accelerate progress through One Hea...Six months after the United Nations General Assembly 2nd high-level meeting in 26 September 2024 to review the progress made at all levels to tackle antimicrobial resistance(AMR)and accelerate progress through One Health,Global AMR Innovation Fund(GAMRIF)summit 2025 was held with three aims:“to encourage and foster new collaboration between AMR research and development(R&D)stakeholders,to share learnings and best practices across the wide range of stakeholders,and to envision the future of AMR R&D”.The summit lasted for three days and began with an introduction and overview of the high-level commitment in AMR and the key global policy developments in 2024 and their implications for innovation R&D(http://globalamrhub.org/news/gamrif-summit-2025/).展开更多
基金funded by SAMRC with grant number RDG2017/18 and the APC was funded by SMU.
文摘Objective:To determine the prevalence of tick-borne pathogens with a particular focus on Rickettsia spp.in ticks collected from cattle in Gauteng and Limpopo Provinces,South Africa.Methods:A total of 200 ticks were collected from cattle within the Madala livestock,Pretoria,Gauteng Province and in Mankweng Township,Polokwane,Limpopo Province in 2019.The ticks were morphologically identified and processed individually for a total genomic DNA extraction.Specific primers targetting ompA,ompB,and the 17KDa genes were used for a molecular screening and delineation of Rickettsia from the extracted genetic materials using polymerase chain reaction(PCR)technique.PCR amplicons of positive samples were sequenced bidirectionally using the Sanger sequencing method.Sequences generated were processed and analysed using appropriate bioinformatics software.Results:The ticks were morphologically identified as Amblyomma spp.PCR profiling of the genomic DNA samples revealed the presence of the Rickettsia pathogen in 42(21%)of the ticks collected from both Provinces.Out of the genes profiled,14(7%)were positive for 17KDa,42(21%)for ompA and 32(16%)were positive for ompB genes respectively.The nucleotide blast of the sequenced genomes showed high similarity,as high as 100% with other reference Rickettsia(R.)africae in the GenBank.The phylogenetic analysis of the sequences further validated them as R.africae with their characteristic clustering pattern with related reference sequences.Conclusions:There is an abundance of R.africae in Amblyomma ticks collected from cattle in the study areas.This has serious public health implications as individuals who accidentally get infested with the ticks could acquire R.africae.Hence,adequate precautions in terms of sensitization of farmers about the risk and mass mobilization drive to control the vectors in the areas are highly recommended to safeguard public health.
基金C.I.received a fungal AMR innovations for LMICs:solutions and access for everyone(FAILSAFE)research grant cofunded by the UK Department of HealthSocial Care(DHSC)as part of the Global AMR Innovation Fund(GAMRIF).One Health UK aid fund that supports research and development around the world to reduce the threat of antimicrobial resistance(AMR)in humans,animals,and the environment for the benefit of people in lowand middle-income countries(LMICs).
文摘Six months after the United Nations General Assembly 2nd high-level meeting in 26 September 2024 to review the progress made at all levels to tackle antimicrobial resistance(AMR)and accelerate progress through One Health,Global AMR Innovation Fund(GAMRIF)summit 2025 was held with three aims:“to encourage and foster new collaboration between AMR research and development(R&D)stakeholders,to share learnings and best practices across the wide range of stakeholders,and to envision the future of AMR R&D”.The summit lasted for three days and began with an introduction and overview of the high-level commitment in AMR and the key global policy developments in 2024 and their implications for innovation R&D(http://globalamrhub.org/news/gamrif-summit-2025/).
