Agarwood is a resinous wood produced by plants of the genus Aquilaria in the Thymelaeaceae family after being injured.The wide distribution of origins and the complexity of formation have led to various species of aga...Agarwood is a resinous wood produced by plants of the genus Aquilaria in the Thymelaeaceae family after being injured.The wide distribution of origins and the complexity of formation have led to various species of agarwood.The preciousness,universality of application and scarcity of resources bring difficulties to the quality control of agarwood,the in-situ ionization technique shows great potential in the rapid and accurate identification of valuable herbal medicines.In this paper,based on the combination of paper capillary spray and miniature mass spectrometry,different kinds of agarwood and counterfeits were directly added onto the chromatography paper,the active ingredients were extracted by solvent and then carried out by the portable miniature mass spectrometry,which could be implemented to perform an effective and on-site analysis,and the whole process only took about 1 min.Characteristic highly oxidized 2-(2-phenylethyl)chromones and flindersia-type 2-(2-phenylethyl)chromones were detected in authentic agarwood,and other characteristic flindersia-type 2-(2-phenylethyl)chromones were detected in authentic Kynam agarwood(special variety of agarwood),while the above characteristic components were not detected in counterfeits.Furthermore,an artificial intelligence model based on random forest algorithm was established for automatic identification.This approach is versatile,unfettered by location or environmental constraints,making it particularly valuable in the identification of precious Chinese herbal medicines.展开更多
基金supported by the National Natural Science Foundation of China(82274223)National Drug Standard Improvement Project(2023Z002)。
文摘Agarwood is a resinous wood produced by plants of the genus Aquilaria in the Thymelaeaceae family after being injured.The wide distribution of origins and the complexity of formation have led to various species of agarwood.The preciousness,universality of application and scarcity of resources bring difficulties to the quality control of agarwood,the in-situ ionization technique shows great potential in the rapid and accurate identification of valuable herbal medicines.In this paper,based on the combination of paper capillary spray and miniature mass spectrometry,different kinds of agarwood and counterfeits were directly added onto the chromatography paper,the active ingredients were extracted by solvent and then carried out by the portable miniature mass spectrometry,which could be implemented to perform an effective and on-site analysis,and the whole process only took about 1 min.Characteristic highly oxidized 2-(2-phenylethyl)chromones and flindersia-type 2-(2-phenylethyl)chromones were detected in authentic agarwood,and other characteristic flindersia-type 2-(2-phenylethyl)chromones were detected in authentic Kynam agarwood(special variety of agarwood),while the above characteristic components were not detected in counterfeits.Furthermore,an artificial intelligence model based on random forest algorithm was established for automatic identification.This approach is versatile,unfettered by location or environmental constraints,making it particularly valuable in the identification of precious Chinese herbal medicines.
