We report about current work which is aimed to improve the adhesion of melt processable elastomers onto relevant reinforcement materials by means of short wave UVC （ultraviolet C） light. Results of laboratory tests ...We report about current work which is aimed to improve the adhesion of melt processable elastomers onto relevant reinforcement materials by means of short wave UVC （ultraviolet C） light. Results of laboratory tests regarding UVC surface activation ofpolyamide fiber materials in air using low-pressure mercury lamps with 185 nm and 254 nm emissions are shown. The effect of irradiation on fiber strength was studied to find out suitable process parameters for providing the UVC treatment efficient but as gentle as possible to avoid negative effects on reinforcement properties. Application of a laboratory process for UVC pretreatment leads to significantly increased adhesion strength between the fibers and the melt processable elastomers on the base of TPA （polyamide） respectively TPU （polyurethane）.展开更多
Suppresssion subtractive hybridization (SSH) was preformed to compare gene expression profiles of PIH patients and normal pregnancy placentas. The subtractive cDNA library of PIH placenta was set up and screedned. Dif...Suppresssion subtractive hybridization (SSH) was preformed to compare gene expression profiles of PIH patients and normal pregnancy placentas. The subtractive cDNA library of PIH placenta was set up and screedned. Differential cDNAs were cloned, and sequenced by T 7 primer methodology. One hundred and three differential cDNAs were isolated by SSH. Sequencing and BLAST analysis showed 90 inserts shared more than 95% homolog with sequences in the GenBank/EMBL database. We identified 36 putative genes including pregnancy-specific glycoprotein gene (BC005924), serine protease inhibitor gene(BC012868), VEGFR-1 gene(AF063657, etc.展开更多
There are multiple guidelines from publishers and organisations on the use of artificial intelligence(AI)in publishing.1–5 However,none are specific to family medicine.Most journals have some basic AI use recommendat...There are multiple guidelines from publishers and organisations on the use of artificial intelligence(AI)in publishing.1–5 However,none are specific to family medicine.Most journals have some basic AI use recommendations for authors,but more explicit direction is needed,as not all AI tools are the same.As family medicine journal editors,we want to provide a unified statement about AI in academic publishing for authors,editors,publishers and peer reviewers based on our current understanding of the field.The technology is advancing rapidly.While text generated from early large language models(LLMs)was relatively easy to identify,text generated from newer versions is getting progressively better at imitating human language and more challenging to detect.展开更多
The Bgl Ⅱ fragment carried in plasmid pMH903,which covers the nodD3 region of Rhizobium meliloti, has been sequenced. By using both S1 nuclease mapping and primer extension, two transcription start sites were demonst...The Bgl Ⅱ fragment carried in plasmid pMH903,which covers the nodD3 region of Rhizobium meliloti, has been sequenced. By using both S1 nuclease mapping and primer extension, two transcription start sites were demonstrated in the sequence. After the first transcription start site, there were two open reading frames (ORF) followed by the nodD3 coding sequence which was also preceded by the second promoter.The nodD3 gene under the first promoter mediated high,constitutive expression of nodC-lacZ fusion,and the gene under the second promoter required the product of nodD1 and alfalfa (Medicago sativa) seed exudate for the activation of fusion.Nodulation experiments showed that the nodD3 gene under either promoter was functional in eliciting nodules on alfalfa.The deletion of part of the two ORFs after the first promoter or deletion of the second promoter did not block the constitutive expression of nodC-lacZ fusion, whereas the deletion of the first promoter region or a polar insertion mutation between the two pr moters did cause nodD3 to activate nodC only in the presence of the inducer.It indicates that nodD3 can be transcribed from the first promoter as well as from a separate second promoter.展开更多
文摘We report about current work which is aimed to improve the adhesion of melt processable elastomers onto relevant reinforcement materials by means of short wave UVC （ultraviolet C） light. Results of laboratory tests regarding UVC surface activation ofpolyamide fiber materials in air using low-pressure mercury lamps with 185 nm and 254 nm emissions are shown. The effect of irradiation on fiber strength was studied to find out suitable process parameters for providing the UVC treatment efficient but as gentle as possible to avoid negative effects on reinforcement properties. Application of a laboratory process for UVC pretreatment leads to significantly increased adhesion strength between the fibers and the melt processable elastomers on the base of TPA （polyamide） respectively TPU （polyurethane）.
基金Supported in part by the National Natural Science Foundation of China (30070787)
文摘Suppresssion subtractive hybridization (SSH) was preformed to compare gene expression profiles of PIH patients and normal pregnancy placentas. The subtractive cDNA library of PIH placenta was set up and screedned. Differential cDNAs were cloned, and sequenced by T 7 primer methodology. One hundred and three differential cDNAs were isolated by SSH. Sequencing and BLAST analysis showed 90 inserts shared more than 95% homolog with sequences in the GenBank/EMBL database. We identified 36 putative genes including pregnancy-specific glycoprotein gene (BC005924), serine protease inhibitor gene(BC012868), VEGFR-1 gene(AF063657, etc.
文摘There are multiple guidelines from publishers and organisations on the use of artificial intelligence(AI)in publishing.1–5 However,none are specific to family medicine.Most journals have some basic AI use recommendations for authors,but more explicit direction is needed,as not all AI tools are the same.As family medicine journal editors,we want to provide a unified statement about AI in academic publishing for authors,editors,publishers and peer reviewers based on our current understanding of the field.The technology is advancing rapidly.While text generated from early large language models(LLMs)was relatively easy to identify,text generated from newer versions is getting progressively better at imitating human language and more challenging to detect.
基金Project supported by the National Natural Science Foundation of China.
文摘The Bgl Ⅱ fragment carried in plasmid pMH903,which covers the nodD3 region of Rhizobium meliloti, has been sequenced. By using both S1 nuclease mapping and primer extension, two transcription start sites were demonstrated in the sequence. After the first transcription start site, there were two open reading frames (ORF) followed by the nodD3 coding sequence which was also preceded by the second promoter.The nodD3 gene under the first promoter mediated high,constitutive expression of nodC-lacZ fusion,and the gene under the second promoter required the product of nodD1 and alfalfa (Medicago sativa) seed exudate for the activation of fusion.Nodulation experiments showed that the nodD3 gene under either promoter was functional in eliciting nodules on alfalfa.The deletion of part of the two ORFs after the first promoter or deletion of the second promoter did not block the constitutive expression of nodC-lacZ fusion, whereas the deletion of the first promoter region or a polar insertion mutation between the two pr moters did cause nodD3 to activate nodC only in the presence of the inducer.It indicates that nodD3 can be transcribed from the first promoter as well as from a separate second promoter.
