Four isolates of Bacillus subtilis coded,B4,B7,B8 and B10 were examined as biocontrol agents for their abilities and antagonistic effect on the in vitro growth of certain phytopathogenic fungi of peanut,Rhizoctonia so...Four isolates of Bacillus subtilis coded,B4,B7,B8 and B10 were examined as biocontrol agents for their abilities and antagonistic effect on the in vitro growth of certain phytopathogenic fungi of peanut,Rhizoctonia solani and Sclerotium rolfsii.Bacillus subtilis isolate B4(GenBank accession no.EF150884)was the highly effective one for inhibiting the fungal mycelial growth.Batch fermentation of B.subtilis isolate B4 was carried out and the maximum biomass achieved was 4.53 g L-1 at 11 h.Bacillus subtilis isolate B4 was formulated and evaluated as a biofungicide to reduce peanut soil-borne diseases under greenhouse and field conditions at the side of Rizolex-T(fungicide)as standard.Treatments by formulated plant growth-promoting rhizobacteria(PGPR)B.subtilis B4 and Rizolex-T in a soil infested with R.solani,S.rolfsii and mixture of them were more effective in decreasing percentage of damping-off,root and pod rot disease incidence(%)in greenhouse and open field environment during the two seasons 2015 and 2016.Treatments by PGPR gave highly dry weight and number of healthy pods compared to control of fungi treatment which was nearby to dry weights of healthy pods achieved by treatments by Rizolex-T in a soil infested with S.rolfsii,R.solani and mixture of them.Formulated PGPR B.subtilis B4 gave higher increasing of yield percentage than treatment by Rizolex-T in the two evaluated seasons 2015 and 2016.It can conclude that the produced bioforumlated agent was more efficient as fungicide when compared with the other chemical synthesized fungicides,safe for human and the environment and economy.展开更多
Identification of quantitative trait loci(QTLs)controlling yield and yield-related traits in rice was performed in the F_(2) mapping population derived from parental rice genotypes DHMAS and K343.A total of 30 QTLs go...Identification of quantitative trait loci(QTLs)controlling yield and yield-related traits in rice was performed in the F_(2) mapping population derived from parental rice genotypes DHMAS and K343.A total of 30 QTLs governing nine different traits were identified using the composite interval mapping(CIM)method.Four QTLs were mapped for number of tillers per plant on chromosomes 1(2 QTLs),2 and 3;three QTLs for panicle number per plant on chromosomes 1(2 QTLs)and 3;four QTLs for plant height on chromosomes 2,4,5 and 6;one QTL for spikelet density on chromosome 5;four QTLs for spikelet fertility percentage(SFP)on chromosomes 2,3 and 5(2 QTLs);two QTLs for grain length on chromosomes 1 and 8;three QTLs for grain width on chromosomes1,3 and 8;three QTLs for 1000-grain weight(TGW)on chromosomes 1,4 and 8 and six QTLs for yield per plant(YPP)on chromosomes 2(3 QTLs),4,6 and 8.Most of the QTLs were detected on chromosome 2,so further studies on chromosome 2 could help unlock some new chapters of QTL for this cross of rice variety.Identified QTLs elucidating high phenotypic variance can be used for marker-assisted selection(MAS)breeding.Further,the exploitation of information regarding molecular markers tightly linked to QTLs governing these traits will facilitate future crop improvement strategies in rice.展开更多
文摘Four isolates of Bacillus subtilis coded,B4,B7,B8 and B10 were examined as biocontrol agents for their abilities and antagonistic effect on the in vitro growth of certain phytopathogenic fungi of peanut,Rhizoctonia solani and Sclerotium rolfsii.Bacillus subtilis isolate B4(GenBank accession no.EF150884)was the highly effective one for inhibiting the fungal mycelial growth.Batch fermentation of B.subtilis isolate B4 was carried out and the maximum biomass achieved was 4.53 g L-1 at 11 h.Bacillus subtilis isolate B4 was formulated and evaluated as a biofungicide to reduce peanut soil-borne diseases under greenhouse and field conditions at the side of Rizolex-T(fungicide)as standard.Treatments by formulated plant growth-promoting rhizobacteria(PGPR)B.subtilis B4 and Rizolex-T in a soil infested with R.solani,S.rolfsii and mixture of them were more effective in decreasing percentage of damping-off,root and pod rot disease incidence(%)in greenhouse and open field environment during the two seasons 2015 and 2016.Treatments by PGPR gave highly dry weight and number of healthy pods compared to control of fungi treatment which was nearby to dry weights of healthy pods achieved by treatments by Rizolex-T in a soil infested with S.rolfsii,R.solani and mixture of them.Formulated PGPR B.subtilis B4 gave higher increasing of yield percentage than treatment by Rizolex-T in the two evaluated seasons 2015 and 2016.It can conclude that the produced bioforumlated agent was more efficient as fungicide when compared with the other chemical synthesized fungicides,safe for human and the environment and economy.
基金supported by the Researchers Supporting Project(RSP-2021/298),King Saud University in Riyadh,Saudi Arabia.
文摘Identification of quantitative trait loci(QTLs)controlling yield and yield-related traits in rice was performed in the F_(2) mapping population derived from parental rice genotypes DHMAS and K343.A total of 30 QTLs governing nine different traits were identified using the composite interval mapping(CIM)method.Four QTLs were mapped for number of tillers per plant on chromosomes 1(2 QTLs),2 and 3;three QTLs for panicle number per plant on chromosomes 1(2 QTLs)and 3;four QTLs for plant height on chromosomes 2,4,5 and 6;one QTL for spikelet density on chromosome 5;four QTLs for spikelet fertility percentage(SFP)on chromosomes 2,3 and 5(2 QTLs);two QTLs for grain length on chromosomes 1 and 8;three QTLs for grain width on chromosomes1,3 and 8;three QTLs for 1000-grain weight(TGW)on chromosomes 1,4 and 8 and six QTLs for yield per plant(YPP)on chromosomes 2(3 QTLs),4,6 and 8.Most of the QTLs were detected on chromosome 2,so further studies on chromosome 2 could help unlock some new chapters of QTL for this cross of rice variety.Identified QTLs elucidating high phenotypic variance can be used for marker-assisted selection(MAS)breeding.Further,the exploitation of information regarding molecular markers tightly linked to QTLs governing these traits will facilitate future crop improvement strategies in rice.
