It has been reported by us that the analgesic effect of acupuncture was reduced following bilateral electrolytic lesions of nucleus accumbens in rats or local 6-hydroxydopamine (6-OH-DA) injection to the nucleus accum...It has been reported by us that the analgesic effect of acupuncture was reduced following bilateral electrolytic lesions of nucleus accumbens in rats or local 6-hydroxydopamine (6-OH-DA) injection to the nucleus accumbens. In order to further elucidate the role played by nucleus accumbeus in the integration of pain information, in the present study the effect of electrical stimulation of nucleus accumbens on the pain reaction was observed in rabbits.展开更多
Previous studies ia our laboratory have shown that hypothalamic arcuate nucleus (ARC),locus coeruleus (LG) and dorsal raphe nucleus (DR) play an important role in pain modulation and acupuncture analgesia, There is su...Previous studies ia our laboratory have shown that hypothalamic arcuate nucleus (ARC),locus coeruleus (LG) and dorsal raphe nucleus (DR) play an important role in pain modulation and acupuncture analgesia, There is sufficient evidence that LG and DR are main sites concentrated with noradrenergic (NE) and serotonergic (5-HT) neuronal peri-展开更多
Parafascicular nucleus (PF) is a structure which receives pain impulses, and it also is an important region where the afferent impulses from acupuncture point and pain area are interrelated and interact on each other.
Electrophysiological studies have shown that acetylcholine may be one of the neurotransmitters in the nucleus isthmi (NI) of amphibians and reptiles, but little (in amphibians) or nothing (in reptiles) is known about ...Electrophysiological studies have shown that acetylcholine may be one of the neurotransmitters in the nucleus isthmi (NI) of amphibians and reptiles, but little (in amphibians) or nothing (in reptiles) is known about the distribution of its hydrolase,acetylcholinesterase (AChE), within NI. However, abundant AChE is a necessary con-展开更多
The determination of the optical luminosity of a galactic nucleus plays an important role in investigating active galactic nuclei. It is a key quantity not only for correlation analyses of various observables of galac...The determination of the optical luminosity of a galactic nucleus plays an important role in investigating active galactic nuclei. It is a key quantity not only for correlation analyses of various observables of galactic nuclei, but also for the study of the proper processes and emission mechanism in active galactic nuclei. In 1971, Sandage展开更多
Ⅰ. INTRODUCTION Caudate nucleus (CN) contains a variety of neurotransmitters, e. g. acetylcholine (Ach), dopamine (DA), endorphins and 5-hydroxytryptamine (5-HT). It proves that CN has played an important role in acu...Ⅰ. INTRODUCTION Caudate nucleus (CN) contains a variety of neurotransmitters, e. g. acetylcholine (Ach), dopamine (DA), endorphins and 5-hydroxytryptamine (5-HT). It proves that CN has played an important role in acupuncture analgesia. Carlsson (1959) found that the CN was the region of central nerves system (CNS) where DA concentration was the highest. The striatal dopaminergie neurons were allegedly related to morphine analgesia. However, whether they also play a role in acupuncture analgesia展开更多
Since the pioneering work by Broca and Wernicke in the 19th century,who examined individuals with brain lesions to associate them with specific behaviors,it was evident that behaviors are complex and cannot be fully a...Since the pioneering work by Broca and Wernicke in the 19th century,who examined individuals with brain lesions to associate them with specific behaviors,it was evident that behaviors are complex and cannot be fully attributable to specific brain areas alone.Instead,they involve connectivity among brain areas,whether close or distant.At that time,this approach was considered the optimal way to dissect brain circuitry and function.These pioneering efforts opened the field to explore the necessity or sufficiency of brain areas in controlling behavior and hence dissecting brain function.However,the connectivity of the brain and the mechanisms through which various brain regions regulate specific behaviors,either individually or collaboratively,remain largely elusive.Utilizing animal models,researchers have endeavored to unravel the necessity or sufficiency of specific brain areas in influencing behavior;however,no clear associations have been firmly established.展开更多
The restoration of European flat oyster (Ostrea edulis) population is critical for biodiversity and ecosystem services recovery. This study evaluates the potential of innovative clay substrates, developed and patented...The restoration of European flat oyster (Ostrea edulis) population is critical for biodiversity and ecosystem services recovery. This study evaluates the potential of innovative clay substrates, developed and patented by Oyster Heaven, as cost-effective and degradable alternatives for large-scale oyster reef restoration. Field trials conducted in an oyster aquaculture site and hatchery experiments under controlled conditions assessed the larvae settlement efficiency, microbial biofilm composition, and environmental influences. Results showed significantly lower larvae recruitment on clay prototypes and spat collectors used as control, in wild conditions, with environmental factors such as temperature and hypoxia playing a key role. In the hatchery, larvae preferred oyster shells (17,174 ± 659 spats/m2) over clay prototypes (2917 ± 111 spats/m2) and spat collectors (1451 ± 160 spats/m2). Microbial analysis revealed a shift towards Cyanobacteria and Woeseia species, with Pseudoalteromonadaceae, a genus previously linked to both stimulating and inhibiting larvae settlement, detected only on clay surfaces. Despite challenges, the clay prototypes demonstrated potential as sustainable substrates for restoration, particularly in managed “spats-on-reefs” strategies. This research highlights the importance of environmental monitoring, microbial interactions, and substrate optimization in enhancing restoration success for O. edulis populations. Further investigations are recommended to refine clay designs and assess their broader applicability under variable environmental conditions.展开更多
Through the analysis of representative samples of field galaxies,both local(z~0.027)and distant(z~0.7),we explore the barred galaxy fraction and its dependence on stellar mass,color,and morphology,aiming to understand...Through the analysis of representative samples of field galaxies,both local(z~0.027)and distant(z~0.7),we explore the barred galaxy fraction and its dependence on stellar mass,color,and morphology,aiming to understand the evolution of these structural components through cosmic time.To this end,two complementary bar detection techniques were employed:elliptical isophote fitting and two-dimensional Fourier analysis,both applied to deep optical images.The observational samples were drawn from previously established and calibrated catalogs to ensure a homogeneous selection in stellar mass,enabling a robust comparison between local galaxies(z~0.027)and those in the distant Universe(z~0.7).This study systematically applies both isophotal fitting and Fourier decomposition across a wide redshift range,offering a comprehensive view of the evolution of bar incidence as a function of stellar mass and morphology.The results indicate that the fraction of barred galaxies is significantly higher in the local Universe than at earlier epochs,particularly among spiral galaxies.Furthermore,a clear correlation is observed between the presence of bars and stellar mass,especially in the high-mass regime(log(M_*/M☉)>10.5).In distant galaxies,this fraction is lower across all mass ranges,which may be related to more active dynamical processes.Overall,the findings reinforce the idea that stellar bars emerge as a consequence of dynamical cooling and the progressive stabilization of galactic disks,playing a key role in gas transport and the internal structural evolution of galaxies from z~1 to the present day.展开更多
This paper proposes an extension of the Modified-Plant ADRC(MP-ADRC)strategy to broaden its application to minimum phase dynamical systems.The main features of the MP-ADRC method are the inclusion of a constant gain i...This paper proposes an extension of the Modified-Plant ADRC(MP-ADRC)strategy to broaden its application to minimum phase dynamical systems.The main features of the MP-ADRC method are the inclusion of a constant gain in series with the plant output error and a linear filter in parallel with the overall error system.These structural changes do not influence the input/output dynamics of the original plant,but are intentionally introduced to modify the dynamics to be estimated by the extended state observer(ESO)and,thus,promote an increase in the robustness of the method.Some advantages can also be attributed to the proposed methodology,such as(i)the design procedures of both the controller and the ESO only require knowledge of the sign(±)of the plant input channel coefficient(or control gain);(ii)the plant control input is generated directly by a single ESO state variable.Despite the advantages and the characteristics of MP-ADRC mentioned earlier,closed-loop stability cannot be guaranteed when it is applied to dynamical systems that have finite zeros.To overcome this difficulty,this work introduces an extension in the MP-ADRC method.It basically consists of rewriting the minimum phase plant dynamics according to its relative order,and then follows with the design of the ESO by conveniently increasing the number of ESO state variables.The simulation results are also presented to illustrate the application of the proposed method.展开更多
AIM: To establish whether virulence factor genes vacA and cagA are present in Helicobacter pylori (H. pylori) retrieved from gastric mucosa and dental plaque in pa-tients with dyspepsia. METHODS: Cumulative dental pla...AIM: To establish whether virulence factor genes vacA and cagA are present in Helicobacter pylori (H. pylori) retrieved from gastric mucosa and dental plaque in pa-tients with dyspepsia. METHODS: Cumulative dental plaque specimens and gastric biopsies were submitted to histological exami-nation, rapid urease test and polymerase chain reac-tion (PCR) assays to detect the presence of cagA and vacA polymorphisms.RESULTS: Detection of H. pylori from dental plaque and gastric biopsy samples was greater by PCR com-pared to histological examination and the rapid ure-ase test. DNA from H. pylori was detected in 96% of gastric mucosa samples and in 72% of dental plaque samples. Sixty-three (89%) of 71 dental plaque sam-ples that were H. pylori-positive also exhibited identical vacA and cagA genotypes in gastric mucosa. The most common genotype was vacAs1bm1 and cagA positive, either in dental plaque or gastric mucosa. These viru-lent H. pylori isolates were involved in the severity of clinical outcome.CONCLUSION: These pathogenic strains were found simultaneously in dental plaque and gastric mucosa, which suggests that gastric infection is correlated with the presence of H. pylori in the mouth.展开更多
Volcanism is a primary process of land formation.It provides a model for understanding soil-forming processes and the role of pioneer bacteria and/or archaea as early colonizers in those new environments.The objective...Volcanism is a primary process of land formation.It provides a model for understanding soil-forming processes and the role of pioneer bacteria and/or archaea as early colonizers in those new environments.The objective of this study was to identify the microbial communities involved in soil formation.DNA was extracted from soil samples from the Llaima volcano in Chile at sites destroyed by lava in different centuries(1640,1751,and 1957).Bacterial and archaeal 16 S r RNA genes were analyzed using quantitative polymerase chain reaction(q PCR)and Illumina Mi Seq sequencing.Results showed that microbial diversity increased with soil age,particularly between the 1751 and 1640 soils.For archaeal communities,Thaumarchaeota was detected in similar abundances in all soils,but Euryarchaeota was rare in the older soils.The analysis of bacterial 16 S r RNA genes showed high abundances of Chloroflexi(37%),Planctomycetes(18%),and Verrucomicrobia(10%)in the youngest soil.Proteobacteria and Acidobacteria were highly abundant in the older soils(16%in 1640 and 15%in 1751 for Acidobacteria;38%in 1640 and 27%in 1751 for Proteobacteria).The microbial profiles in the youngest soils were unusual,with a high abundance of bacteria belonging to the order Ktedonobacterales(Chloroflexi)in the 1957 soil(37%)compared with the 1751(18%)and 1640(7%)soils.In this study,we show that there is a gradual establishment of the microbial community in volcanic soils following an eruption and that specific microbial groups can colonize during the early stages of recovery.展开更多
Glaciers are extensively developed in the southwest of Tibet and the moraines are widely distributed with large depth. Large-scale debris flows are often reported which blocked rivers and formed dams. In this paper, s...Glaciers are extensively developed in the southwest of Tibet and the moraines are widely distributed with large depth. Large-scale debris flows are often reported which blocked rivers and formed dams. In this paper, seven large debris flows in four valleys are discussed, among which five dams developed. 13 sets of experiments have been conducted in laboratory to simulate the formation and failure of the dam. Finally, a model of dam failure is proposed and a formula is established to calculate the flood discharge： Q=kbnnk/TB^-/LG^0.1,where bk is the outlet width of the dam at the original water level, hk the erosive depth, T the time from overflow to final state of failure, the average width of lake; L the length of the lake, and G the total potential energy of the water in the lake.展开更多
Objective: To evaluate the antioxidant activity of the essential oil obtained from Rosmarinus officinalis(R. officinalis) in ethanol-induced gastric ulcer model in vivo.Methods: The antioxidant properties of the essen...Objective: To evaluate the antioxidant activity of the essential oil obtained from Rosmarinus officinalis(R. officinalis) in ethanol-induced gastric ulcer model in vivo.Methods: The antioxidant properties of the essential oil obtained from R. officinalis were evaluated against gastric injury induced by absolute ethanol. Gastric tissues were prepared to enzymatic assays. The levels of glutathione, lipid peroxides, and the activities of glutathione peroxidase, superoxide dismutase and myeloperoxidase were measured.Results: Ethanol produced severe hemorrhagic lesions in the stomach with ulcerative lesion of(140.2 ± 37.2) mm2. In animals pretreated with essential oil of R. officinalis(50 mg/kg, p.o.), a significant inhibition of mucosal injury of(21.2 ± 7.1) mm2(84%inhibition) was observed. The essential oil of R. officinalis protected the gastric mucosa probably by modulating the activities of the enzymes(superoxide dismutase and glutathione peroxidase) and increasing or maintaining the levels of glutathione. In addition,lipid peroxides levels were reduced. The essential oil of R. officinalis was analyzed by gas chromatography–mass spectrometer and the main constituents were cineole(28.5%),camphor(27.7%) and alpha-pinene(21.3%).Conclusions: We suggest that the monoterpenes present in the essential oil obtained from R. officinalis may be among the active principles responsible for the antioxidant activity shown by essential oil of R. officinalis.展开更多
AIM： To study the association between Interleukin-1 （IL-1） and tumor necrosis factor （TNF）-α polymorphisms, infection by Helicobacter pylori （H pylori） and the development of gastrointestinal diseases.METHODS...AIM： To study the association between Interleukin-1 （IL-1） and tumor necrosis factor （TNF）-α polymorphisms, infection by Helicobacter pylori （H pylori） and the development of gastrointestinal diseases.METHODS： Genomic DNA was extracted from the peripheral blood of 177 patients with various gastrointestinal diseases and from 100 healthy volunteers. The polymorphisms in IL-1β and TNF-α genes were analyzed using the polymerase chain reactionrestriction fragment length polymorphism method （PCRRFLP） and those from IL-1RN with PCR. The presence of infection due to H pylori and the presence of the CagA toxin were detected by serology. The histopathological parameters in the gastric biopsies of the patients were according to the Sydney classification.RESULTS： A comparison of the frequencies of the different polymorphisms studied among the patients and the control group demonstrated that the allele IL- 1RN＊2 was more frequent among patients with gastric ulcers and adenocarcinoma. Carriers of the allele IL- RN＊2 and those with reactive serology for anti-CagA IgG had a greater risk of developing peptic ulcer and gastric adenocarcinoma, as well as a higher degree of inflammation and neutrophilic activity in the gastricCONCLUSION： Our results indicate a positive association between IL-1RN gene polymorphism and infection by positive H pylori CagA strains and the development of gastric ulcers and adenocarcinoma.展开更多
The effect of soya lecithin (SL) on solubilization and biodegradation of pentachlorophenol (PCP) by Anthracophyllum discolor was evaluated. Besides, the PCP effect on critical micelle concentration (CMC) of SL and the...The effect of soya lecithin (SL) on solubilization and biodegradation of pentachlorophenol (PCP) by Anthracophyllum discolor was evaluated. Besides, the PCP effect on critical micelle concentration (CMC) of SL and the effect of PCP and SL on the ligninolytic activity of A. discolor were evaluated. The PCP solubilization test was performed for 100, 200 and 400 mg·L-1 with SL solution varying concentrations of ranging from 0 to 0.9 g·L-1. The effect of PCP (5 to 20 mg·L-1) on CMC of SL by conductivity was evaluated. The effect of SL (0 to 0.9 g·L-1) on biodegradation of PCP (21.4 mg·L-1) in modified Kirk medium was studied. The results showed that SL addition enhanced PCP solubility in water. The solubilities of 100, 200 and 400 mg·L-1 of PCP were about 4.2, 5.0 and 2.5 times higher than their solubility in pure water when the system was added with 0.9 g·L-1 of SL. We found that the increase in PCP concentration caused a decrease in the CMC value. In the biodegradation assay, in liquid medium supplemented with 0.9 g·L-1 of SL, the 94.4% of PCP was biodegraded after 26 days of incubation. In contrast, in the control assay (without SL), the 76.5% of PCP was removed. The PCP degradation was confirmed by the presence of its metabolites. During biodegradation assay, A. discolor mainly produced manganese peroxidase reaching a maximum of 96.8 U·g-1 (dw) when the culture medium was added with 0.6 g·L-1 of SL. These results show that SL can be applied to increasing the bioavailability and biodegradation of PCP.展开更多
AIM: To characterize APC gene mutations and correlate them with patient phenotypes in individuals diagnosed with familial adenomatous polyposis(FAP) in northern Brazil. METHODS: A total of 15 individuals diagnosed wit...AIM: To characterize APC gene mutations and correlate them with patient phenotypes in individuals diagnosed with familial adenomatous polyposis(FAP) in northern Brazil. METHODS: A total of 15 individuals diagnosed with FAP from 5 different families from the north of Brazil were analyzed in this study. In addition to patients with histopathological diagnosis of FAP,family members who had not developed the disease were also tested in order to identify mutations and for possible genetic counseling. All analyzed patients or their guardians signed a consent form approved by the Research Ethics Committee of the Jo?o de Barros Barreto University Hospital(Belem,Brazil). DNA extracted from the peripheral blood of a member of each of the affected families was subjected to direct sequencing. The proband of each family was sequenced to identify germline mutations using the Ion Torrent platform. To validate the detected mutations,Sanger sequencing was also performed. The samples from all patients were also tested for the identification of mutations by real-time quantitative polymerase chain reaction using the amplification refractory mutation system. RESULTS: Through interviews with relatives and a search of medical records,it was possible to construct genograms for three of the five families included in the study. All 15 patients from the five families with FAP exhibited mutations in the APC gene,and all mutations were detected in exon 15 of the APC gene. In addition to the patients with a histological diagnosis of FAP,family members without disease symptoms showed the mutation in the APC gene. In the present study,we detected two of the three most frequent germline mutations in the literature: the mutation at codon 1309 and the mutation at codon 1061. The presence of c.3956 del C mutation was found in all families from this study,and suggests that this mutation was introduced in the population of the State of Pará through ancestor immigration(i.e.,a de novo mutation that arose in one member belonging to this state from Brazil). CONCLUSION: Regardless of its origin,the c.3956 del C mutation is a strong candidate biomarker of this hereditary cancer syndrome in families of northern Brazil.展开更多
The spraying of herbicides in crops has become the main form of weed control. Although it means unexpected effects on non-target plants resulted by spraying drift. Thus, improvements in application techniques, as the ...The spraying of herbicides in crops has become the main form of weed control. Although it means unexpected effects on non-target plants resulted by spraying drift. Thus, improvements in application techniques, as the best selection of spray nozzles and adjuvant, are essential to avoid environmental contamination and economic losses. On this work, we evaluate how much adjuvant associated with nozzles can reduce the spray drift. The nozzles used at experiment were air induction flat tip, hollow cone and twinjet and the spray liquids, which were composed of herbicide glyphosate and phosphatidylcholine + propionic acid adjuvant. Measurements were made at wind tunnel and droplet sizer, at laser diffraction method. The models of nozzles influence in droplet size characteristics and in occurrence of spray drift. The use of adjuvants reduces the spray drift only combined with the twinjet nozzle, while for the other models the adjuvant did not reduce the global spray drift at significant levels. The adjuvant reduced the spray drift until 39%, while the nozzles model reduced until 74%. Both techniques when combined were able to reduce until 80%. The model of nozzle has the biggest result on drift mitigation and the use of adjuvants can increase the drift mitigation specially with nozzles that produces smallest droplets.展开更多
The main goal of this research study was to establish the physicochemical characteristics of sewage sludge, and the nutrients needed to enhance the properties of soil with a view to exploiting were potential for agric...The main goal of this research study was to establish the physicochemical characteristics of sewage sludge, and the nutrients needed to enhance the properties of soil with a view to exploiting were potential for agriculture. The example used was the cultivation of radish (Raphanus sativus). The findings are intended as a contribution towards making agriculture sustainable. This study was carried out to assess the response of radish (Raphanus sativus L.) to doses of sewage sludge applied in medium texture soils, and the socioeconomic contributions that such cultivation of radish would make. Levels of 0.25 and 75 ton/ha of sewage sludge plus the equivalent dose of 25 ton/ha of sewage sludge vermin compost by California Red were used after making a randomized experimental design with three replicates. 30 days after sowing radish seeds, the following data were collected: the number of leaves per plant (NLP);plant height (PH in cm);root diameter (RD) and tubercle production (TP ton/ha). In addition, the pH, EC-electric conductivity (EC), and soil organic matter (SOM) were determined. Statistical analysis showed that 25 and 50 tons/ha gave the best results for the parameters assessed. The estimated increase in family mean income from selling radish crops fertilized with sewage sludge is around 17%.展开更多
Phase and amplitude regeneration are necessary for degraded differential phase-shift keying communication sys- tems. This paper proposes a regenerator based on semiconductor optical amplifier for differential phase-sh...Phase and amplitude regeneration are necessary for degraded differential phase-shift keying communication sys- tems. This paper proposes a regenerator based on semiconductor optical amplifier for differential phase-shift keying signals. The key regeneration mechanism is theoretically analysed. The effectiveness of semiconductor optical amplifier based regenerator is demonstrated by comparing the bit error rate and eye diagrams before and after regeneration for 40-Cbit/s differential phase-shift keying 1080-km transmission systems. The results show that regeneration effects are very well. Bit error rate is tess than 10-12 with the regenerator.展开更多
文摘It has been reported by us that the analgesic effect of acupuncture was reduced following bilateral electrolytic lesions of nucleus accumbens in rats or local 6-hydroxydopamine (6-OH-DA) injection to the nucleus accumbens. In order to further elucidate the role played by nucleus accumbeus in the integration of pain information, in the present study the effect of electrical stimulation of nucleus accumbens on the pain reaction was observed in rabbits.
基金Project supported by the Science Fund of Academia Sinica (82, No. 100).
文摘Previous studies ia our laboratory have shown that hypothalamic arcuate nucleus (ARC),locus coeruleus (LG) and dorsal raphe nucleus (DR) play an important role in pain modulation and acupuncture analgesia, There is sufficient evidence that LG and DR are main sites concentrated with noradrenergic (NE) and serotonergic (5-HT) neuronal peri-
文摘Parafascicular nucleus (PF) is a structure which receives pain impulses, and it also is an important region where the afferent impulses from acupuncture point and pain area are interrelated and interact on each other.
文摘Electrophysiological studies have shown that acetylcholine may be one of the neurotransmitters in the nucleus isthmi (NI) of amphibians and reptiles, but little (in amphibians) or nothing (in reptiles) is known about the distribution of its hydrolase,acetylcholinesterase (AChE), within NI. However, abundant AChE is a necessary con-
文摘The determination of the optical luminosity of a galactic nucleus plays an important role in investigating active galactic nuclei. It is a key quantity not only for correlation analyses of various observables of galactic nuclei, but also for the study of the proper processes and emission mechanism in active galactic nuclei. In 1971, Sandage
文摘Ⅰ. INTRODUCTION Caudate nucleus (CN) contains a variety of neurotransmitters, e. g. acetylcholine (Ach), dopamine (DA), endorphins and 5-hydroxytryptamine (5-HT). It proves that CN has played an important role in acupuncture analgesia. Carlsson (1959) found that the CN was the region of central nerves system (CNS) where DA concentration was the highest. The striatal dopaminergie neurons were allegedly related to morphine analgesia. However, whether they also play a role in acupuncture analgesia
基金supported by ANID Fondecyt Iniciacion 11180540(to FJB)ANID PAI 77180077(to FJB)+2 种基金UNAB DI-02-22/REG(to FJB)Exploración-ANID 13220203(to FJB)ANID-MILENIO(NCN2023_23,to FJB)。
文摘Since the pioneering work by Broca and Wernicke in the 19th century,who examined individuals with brain lesions to associate them with specific behaviors,it was evident that behaviors are complex and cannot be fully attributable to specific brain areas alone.Instead,they involve connectivity among brain areas,whether close or distant.At that time,this approach was considered the optimal way to dissect brain circuitry and function.These pioneering efforts opened the field to explore the necessity or sufficiency of brain areas in controlling behavior and hence dissecting brain function.However,the connectivity of the brain and the mechanisms through which various brain regions regulate specific behaviors,either individually or collaboratively,remain largely elusive.Utilizing animal models,researchers have endeavored to unravel the necessity or sufficiency of specific brain areas in influencing behavior;however,no clear associations have been firmly established.
文摘The restoration of European flat oyster (Ostrea edulis) population is critical for biodiversity and ecosystem services recovery. This study evaluates the potential of innovative clay substrates, developed and patented by Oyster Heaven, as cost-effective and degradable alternatives for large-scale oyster reef restoration. Field trials conducted in an oyster aquaculture site and hatchery experiments under controlled conditions assessed the larvae settlement efficiency, microbial biofilm composition, and environmental influences. Results showed significantly lower larvae recruitment on clay prototypes and spat collectors used as control, in wild conditions, with environmental factors such as temperature and hypoxia playing a key role. In the hatchery, larvae preferred oyster shells (17,174 ± 659 spats/m2) over clay prototypes (2917 ± 111 spats/m2) and spat collectors (1451 ± 160 spats/m2). Microbial analysis revealed a shift towards Cyanobacteria and Woeseia species, with Pseudoalteromonadaceae, a genus previously linked to both stimulating and inhibiting larvae settlement, detected only on clay surfaces. Despite challenges, the clay prototypes demonstrated potential as sustainable substrates for restoration, particularly in managed “spats-on-reefs” strategies. This research highlights the importance of environmental monitoring, microbial interactions, and substrate optimization in enhancing restoration success for O. edulis populations. Further investigations are recommended to refine clay designs and assess their broader applicability under variable environmental conditions.
基金financial support from the Secretaría Nacional de Ciencia,Tecnologia e Innovacion(SENACYT),through the Departamento de Fortalecimiento a los Posgrados Nacionalesthe coordination of the program"MOVILIDAD DE INVESTIGACION."+3 种基金the financial support provided by PAPIIT projects IN108323 and IN111825 from DGAPA-UNAMsupport from the Agencia Nacional de Investigación y Desarrollo(ANID)through Basal project FB210003FONDECYT Regular projects 1241426 and 123044Millennium Science Initiative Program NCN2024_112。
文摘Through the analysis of representative samples of field galaxies,both local(z~0.027)and distant(z~0.7),we explore the barred galaxy fraction and its dependence on stellar mass,color,and morphology,aiming to understand the evolution of these structural components through cosmic time.To this end,two complementary bar detection techniques were employed:elliptical isophote fitting and two-dimensional Fourier analysis,both applied to deep optical images.The observational samples were drawn from previously established and calibrated catalogs to ensure a homogeneous selection in stellar mass,enabling a robust comparison between local galaxies(z~0.027)and those in the distant Universe(z~0.7).This study systematically applies both isophotal fitting and Fourier decomposition across a wide redshift range,offering a comprehensive view of the evolution of bar incidence as a function of stellar mass and morphology.The results indicate that the fraction of barred galaxies is significantly higher in the local Universe than at earlier epochs,particularly among spiral galaxies.Furthermore,a clear correlation is observed between the presence of bars and stellar mass,especially in the high-mass regime(log(M_*/M☉)>10.5).In distant galaxies,this fraction is lower across all mass ranges,which may be related to more active dynamical processes.Overall,the findings reinforce the idea that stellar bars emerge as a consequence of dynamical cooling and the progressive stabilization of galactic disks,playing a key role in gas transport and the internal structural evolution of galaxies from z~1 to the present day.
基金supported in part by the Brazilian research agencies CNPq and CAPESby the Fundação Carlos Chagas Filho de AmparoàPesquisa do Estado do Rio de Janeiro,FAPERJ-Brasil(Project E-26/210.425/2024).
文摘This paper proposes an extension of the Modified-Plant ADRC(MP-ADRC)strategy to broaden its application to minimum phase dynamical systems.The main features of the MP-ADRC method are the inclusion of a constant gain in series with the plant output error and a linear filter in parallel with the overall error system.These structural changes do not influence the input/output dynamics of the original plant,but are intentionally introduced to modify the dynamics to be estimated by the extended state observer(ESO)and,thus,promote an increase in the robustness of the method.Some advantages can also be attributed to the proposed methodology,such as(i)the design procedures of both the controller and the ESO only require knowledge of the sign(±)of the plant input channel coefficient(or control gain);(ii)the plant control input is generated directly by a single ESO state variable.Despite the advantages and the characteristics of MP-ADRC mentioned earlier,closed-loop stability cannot be guaranteed when it is applied to dynamical systems that have finite zeros.To overcome this difficulty,this work introduces an extension in the MP-ADRC method.It basically consists of rewriting the minimum phase plant dynamics according to its relative order,and then follows with the design of the ESO by conveniently increasing the number of ESO state variables.The simulation results are also presented to illustrate the application of the proposed method.
基金Supported by Coordenao de Aperfeioamento de Pessoal de Nível Superior and Federal University of Pará
文摘AIM: To establish whether virulence factor genes vacA and cagA are present in Helicobacter pylori (H. pylori) retrieved from gastric mucosa and dental plaque in pa-tients with dyspepsia. METHODS: Cumulative dental plaque specimens and gastric biopsies were submitted to histological exami-nation, rapid urease test and polymerase chain reac-tion (PCR) assays to detect the presence of cagA and vacA polymorphisms.RESULTS: Detection of H. pylori from dental plaque and gastric biopsy samples was greater by PCR com-pared to histological examination and the rapid ure-ase test. DNA from H. pylori was detected in 96% of gastric mucosa samples and in 72% of dental plaque samples. Sixty-three (89%) of 71 dental plaque sam-ples that were H. pylori-positive also exhibited identical vacA and cagA genotypes in gastric mucosa. The most common genotype was vacAs1bm1 and cagA positive, either in dental plaque or gastric mucosa. These viru-lent H. pylori isolates were involved in the severity of clinical outcome.CONCLUSION: These pathogenic strains were found simultaneously in dental plaque and gastric mucosa, which suggests that gastric infection is correlated with the presence of H. pylori in the mouth.
文摘Volcanism is a primary process of land formation.It provides a model for understanding soil-forming processes and the role of pioneer bacteria and/or archaea as early colonizers in those new environments.The objective of this study was to identify the microbial communities involved in soil formation.DNA was extracted from soil samples from the Llaima volcano in Chile at sites destroyed by lava in different centuries(1640,1751,and 1957).Bacterial and archaeal 16 S r RNA genes were analyzed using quantitative polymerase chain reaction(q PCR)and Illumina Mi Seq sequencing.Results showed that microbial diversity increased with soil age,particularly between the 1751 and 1640 soils.For archaeal communities,Thaumarchaeota was detected in similar abundances in all soils,but Euryarchaeota was rare in the older soils.The analysis of bacterial 16 S r RNA genes showed high abundances of Chloroflexi(37%),Planctomycetes(18%),and Verrucomicrobia(10%)in the youngest soil.Proteobacteria and Acidobacteria were highly abundant in the older soils(16%in 1640 and 15%in 1751 for Acidobacteria;38%in 1640 and 27%in 1751 for Proteobacteria).The microbial profiles in the youngest soils were unusual,with a high abundance of bacteria belonging to the order Ktedonobacterales(Chloroflexi)in the 1957 soil(37%)compared with the 1751(18%)and 1640(7%)soils.In this study,we show that there is a gradual establishment of the microbial community in volcanic soils following an eruption and that specific microbial groups can colonize during the early stages of recovery.
基金the National Natural Science Foundation of China(40371016) the West Transportation Construction Science and Tech-nology Project of Ministry of Communications (2006 31879284)
文摘Glaciers are extensively developed in the southwest of Tibet and the moraines are widely distributed with large depth. Large-scale debris flows are often reported which blocked rivers and formed dams. In this paper, seven large debris flows in four valleys are discussed, among which five dams developed. 13 sets of experiments have been conducted in laboratory to simulate the formation and failure of the dam. Finally, a model of dam failure is proposed and a formula is established to calculate the flood discharge： Q=kbnnk/TB^-/LG^0.1,where bk is the outlet width of the dam at the original water level, hk the erosive depth, T the time from overflow to final state of failure, the average width of lake; L the length of the lake, and G the total potential energy of the water in the lake.
基金Supported by Sao Paulo Research Foundation(Grant Number:10/16965-7)National Council for Scientific and Technological Development(Grant Number:303029/2011-0)
文摘Objective: To evaluate the antioxidant activity of the essential oil obtained from Rosmarinus officinalis(R. officinalis) in ethanol-induced gastric ulcer model in vivo.Methods: The antioxidant properties of the essential oil obtained from R. officinalis were evaluated against gastric injury induced by absolute ethanol. Gastric tissues were prepared to enzymatic assays. The levels of glutathione, lipid peroxides, and the activities of glutathione peroxidase, superoxide dismutase and myeloperoxidase were measured.Results: Ethanol produced severe hemorrhagic lesions in the stomach with ulcerative lesion of(140.2 ± 37.2) mm2. In animals pretreated with essential oil of R. officinalis(50 mg/kg, p.o.), a significant inhibition of mucosal injury of(21.2 ± 7.1) mm2(84%inhibition) was observed. The essential oil of R. officinalis protected the gastric mucosa probably by modulating the activities of the enzymes(superoxide dismutase and glutathione peroxidase) and increasing or maintaining the levels of glutathione. In addition,lipid peroxides levels were reduced. The essential oil of R. officinalis was analyzed by gas chromatography–mass spectrometer and the main constituents were cineole(28.5%),camphor(27.7%) and alpha-pinene(21.3%).Conclusions: We suggest that the monoterpenes present in the essential oil obtained from R. officinalis may be among the active principles responsible for the antioxidant activity shown by essential oil of R. officinalis.
基金Supported by Secretaria Executiva de Ciência,Tecnologiae Meio Ambiente-SECTAM and Coordenao de Aperfeioamento de Pessoal de Nível Superior-CAPES
文摘AIM： To study the association between Interleukin-1 （IL-1） and tumor necrosis factor （TNF）-α polymorphisms, infection by Helicobacter pylori （H pylori） and the development of gastrointestinal diseases.METHODS： Genomic DNA was extracted from the peripheral blood of 177 patients with various gastrointestinal diseases and from 100 healthy volunteers. The polymorphisms in IL-1β and TNF-α genes were analyzed using the polymerase chain reactionrestriction fragment length polymorphism method （PCRRFLP） and those from IL-1RN with PCR. The presence of infection due to H pylori and the presence of the CagA toxin were detected by serology. The histopathological parameters in the gastric biopsies of the patients were according to the Sydney classification.RESULTS： A comparison of the frequencies of the different polymorphisms studied among the patients and the control group demonstrated that the allele IL- 1RN＊2 was more frequent among patients with gastric ulcers and adenocarcinoma. Carriers of the allele IL- RN＊2 and those with reactive serology for anti-CagA IgG had a greater risk of developing peptic ulcer and gastric adenocarcinoma, as well as a higher degree of inflammation and neutrophilic activity in the gastricCONCLUSION： Our results indicate a positive association between IL-1RN gene polymorphism and infection by positive H pylori CagA strains and the development of gastric ulcers and adenocarcinoma.
文摘The effect of soya lecithin (SL) on solubilization and biodegradation of pentachlorophenol (PCP) by Anthracophyllum discolor was evaluated. Besides, the PCP effect on critical micelle concentration (CMC) of SL and the effect of PCP and SL on the ligninolytic activity of A. discolor were evaluated. The PCP solubilization test was performed for 100, 200 and 400 mg·L-1 with SL solution varying concentrations of ranging from 0 to 0.9 g·L-1. The effect of PCP (5 to 20 mg·L-1) on CMC of SL by conductivity was evaluated. The effect of SL (0 to 0.9 g·L-1) on biodegradation of PCP (21.4 mg·L-1) in modified Kirk medium was studied. The results showed that SL addition enhanced PCP solubility in water. The solubilities of 100, 200 and 400 mg·L-1 of PCP were about 4.2, 5.0 and 2.5 times higher than their solubility in pure water when the system was added with 0.9 g·L-1 of SL. We found that the increase in PCP concentration caused a decrease in the CMC value. In the biodegradation assay, in liquid medium supplemented with 0.9 g·L-1 of SL, the 94.4% of PCP was biodegraded after 26 days of incubation. In contrast, in the control assay (without SL), the 76.5% of PCP was removed. The PCP degradation was confirmed by the presence of its metabolites. During biodegradation assay, A. discolor mainly produced manganese peroxidase reaching a maximum of 96.8 U·g-1 (dw) when the culture medium was added with 0.6 g·L-1 of SL. These results show that SL can be applied to increasing the bioavailability and biodegradation of PCP.
基金Supported by Grants from Conselho Nacional de Desenvolvimento Científico e Tecnológico(www.cnpq.br),No.401976/2010-6 and No.305220/2013-6(to Burbano RR)Coordenacao de Aperfeicoamento de Pessoal de Nível Superior(www.capes.gov.br),No.PNPD 2810/2011(to Moreira-Nunes CA)
文摘AIM: To characterize APC gene mutations and correlate them with patient phenotypes in individuals diagnosed with familial adenomatous polyposis(FAP) in northern Brazil. METHODS: A total of 15 individuals diagnosed with FAP from 5 different families from the north of Brazil were analyzed in this study. In addition to patients with histopathological diagnosis of FAP,family members who had not developed the disease were also tested in order to identify mutations and for possible genetic counseling. All analyzed patients or their guardians signed a consent form approved by the Research Ethics Committee of the Jo?o de Barros Barreto University Hospital(Belem,Brazil). DNA extracted from the peripheral blood of a member of each of the affected families was subjected to direct sequencing. The proband of each family was sequenced to identify germline mutations using the Ion Torrent platform. To validate the detected mutations,Sanger sequencing was also performed. The samples from all patients were also tested for the identification of mutations by real-time quantitative polymerase chain reaction using the amplification refractory mutation system. RESULTS: Through interviews with relatives and a search of medical records,it was possible to construct genograms for three of the five families included in the study. All 15 patients from the five families with FAP exhibited mutations in the APC gene,and all mutations were detected in exon 15 of the APC gene. In addition to the patients with a histological diagnosis of FAP,family members without disease symptoms showed the mutation in the APC gene. In the present study,we detected two of the three most frequent germline mutations in the literature: the mutation at codon 1309 and the mutation at codon 1061. The presence of c.3956 del C mutation was found in all families from this study,and suggests that this mutation was introduced in the population of the State of Pará through ancestor immigration(i.e.,a de novo mutation that arose in one member belonging to this state from Brazil). CONCLUSION: Regardless of its origin,the c.3956 del C mutation is a strong candidate biomarker of this hereditary cancer syndrome in families of northern Brazil.
文摘The spraying of herbicides in crops has become the main form of weed control. Although it means unexpected effects on non-target plants resulted by spraying drift. Thus, improvements in application techniques, as the best selection of spray nozzles and adjuvant, are essential to avoid environmental contamination and economic losses. On this work, we evaluate how much adjuvant associated with nozzles can reduce the spray drift. The nozzles used at experiment were air induction flat tip, hollow cone and twinjet and the spray liquids, which were composed of herbicide glyphosate and phosphatidylcholine + propionic acid adjuvant. Measurements were made at wind tunnel and droplet sizer, at laser diffraction method. The models of nozzles influence in droplet size characteristics and in occurrence of spray drift. The use of adjuvants reduces the spray drift only combined with the twinjet nozzle, while for the other models the adjuvant did not reduce the global spray drift at significant levels. The adjuvant reduced the spray drift until 39%, while the nozzles model reduced until 74%. Both techniques when combined were able to reduce until 80%. The model of nozzle has the biggest result on drift mitigation and the use of adjuvants can increase the drift mitigation specially with nozzles that produces smallest droplets.
文摘The main goal of this research study was to establish the physicochemical characteristics of sewage sludge, and the nutrients needed to enhance the properties of soil with a view to exploiting were potential for agriculture. The example used was the cultivation of radish (Raphanus sativus). The findings are intended as a contribution towards making agriculture sustainable. This study was carried out to assess the response of radish (Raphanus sativus L.) to doses of sewage sludge applied in medium texture soils, and the socioeconomic contributions that such cultivation of radish would make. Levels of 0.25 and 75 ton/ha of sewage sludge plus the equivalent dose of 25 ton/ha of sewage sludge vermin compost by California Red were used after making a randomized experimental design with three replicates. 30 days after sowing radish seeds, the following data were collected: the number of leaves per plant (NLP);plant height (PH in cm);root diameter (RD) and tubercle production (TP ton/ha). In addition, the pH, EC-electric conductivity (EC), and soil organic matter (SOM) were determined. Statistical analysis showed that 25 and 50 tons/ha gave the best results for the parameters assessed. The estimated increase in family mean income from selling radish crops fertilized with sewage sludge is around 17%.
基金supported by the Scientific Fund for Chinese Universities (Grant No. BUPT 2009RC0413)the National "863" High Technology Projects (Grant No. 2009AA01Z224)
文摘Phase and amplitude regeneration are necessary for degraded differential phase-shift keying communication sys- tems. This paper proposes a regenerator based on semiconductor optical amplifier for differential phase-shift keying signals. The key regeneration mechanism is theoretically analysed. The effectiveness of semiconductor optical amplifier based regenerator is demonstrated by comparing the bit error rate and eye diagrams before and after regeneration for 40-Cbit/s differential phase-shift keying 1080-km transmission systems. The results show that regeneration effects are very well. Bit error rate is tess than 10-12 with the regenerator.
