Polystyrene nanoparticles pose significant toxicological risks to aquatic ecosystems,yet their impact on zebrafish(Danio rerio)embryonic development,particularly erythropoiesis,remains underexplored.This study used si...Polystyrene nanoparticles pose significant toxicological risks to aquatic ecosystems,yet their impact on zebrafish(Danio rerio)embryonic development,particularly erythropoiesis,remains underexplored.This study used single-cell RNA sequencing to comprehensively evaluate the effects of polystyrene nanoparticle exposure on erythropoiesis in zebrafish embryos.In vivo validation experiments corroborated the transcriptomic findings,revealing that polystyrene nanoparticle exposure disrupted erythrocyte differentiation,as evidenced by the decrease in mature erythrocytes and concomitant increase in immature erythrocytes.Additionally,impaired heme synthesis further contributed to the diminished erythrocyte population.These findings underscore the toxic effects of polystyrene nanoparticles on hematopoietic processes,highlighting their potential to compromise organismal health in aquatic environments.展开更多
Peptide metabolites are emerging biomolecules with numerous possibilities in biomaterial-based regenerative medicine due to their inherent bioactivities.These small,naturally occurring compounds are intermediates or b...Peptide metabolites are emerging biomolecules with numerous possibilities in biomaterial-based regenerative medicine due to their inherent bioactivities.These small,naturally occurring compounds are intermediates or byproducts of larger proteins and peptides,and they can have profound effects,such as antiviral therapeutics,proangiogenic agents,and regenerative medicinal applications.This study is among the first to focus on using thymosinβ4 protein-derived metabolites to pioneer novel applications for peptide metabolites in biomaterials.This study found that the novel peptide metabolite acetyl-thymosinβ4(amino acid 1-17)(Ac-Tβ_(1-17))exhibited significant protease inhibition activity against SARS-CoV-2,surpassing its precursor protein.Additionally,Ac-Tβ_(1-17) demonstrated beneficial effects,such as cell proliferation,wound healing,and scavenging of reactive oxygen species(ROS)in human umbilical vein endothelial cells(HUVEC).Integrating Ac-Tβ_(1-17) into a peptide-based scaffold facilitated cell growth and angiogenesis inside the scaffold and through gradual release into the surrounding environment.The Ac-Tβ_(1-17) peptide treatment induced significant biochemical responses in HUVEC,increasing Akt,ERK,PI3K,MEK,and Bcl-2 gene expression and proangiogenic proteins.Ac-Tβ_(1-17) peptide treatment showed similar results in ex vivo by enhancing mouse fetal metatarsal growth and angiogenesis.These findings highlight the potential of natural protein metabolites to generate biologically active peptides,offering a novel strategy for enhancing biomaterial compatibility.This approach holds promise for developing therapeutic biomaterials using peptide metabolites,presenting exciting prospects for future research and applications.展开更多
基金supported by the Institute for Basic Science (IBS-R022-D1)Global Learning&Academic Research Institution for Master’s/Ph D students and Post-Doc Program of the National Research Foundation of Korea Grant funded by the Ministry of Education (RS-2023-00301938)+1 种基金National Research Foundation of Korea Grant funded by the Korean government (RS-2024-00406152,MSIT)Additional financial support was provided by the 2024 Post-Doc Development Program of Pusan National University,Korea Medical Institute,and KREONET。
文摘Polystyrene nanoparticles pose significant toxicological risks to aquatic ecosystems,yet their impact on zebrafish(Danio rerio)embryonic development,particularly erythropoiesis,remains underexplored.This study used single-cell RNA sequencing to comprehensively evaluate the effects of polystyrene nanoparticle exposure on erythropoiesis in zebrafish embryos.In vivo validation experiments corroborated the transcriptomic findings,revealing that polystyrene nanoparticle exposure disrupted erythrocyte differentiation,as evidenced by the decrease in mature erythrocytes and concomitant increase in immature erythrocytes.Additionally,impaired heme synthesis further contributed to the diminished erythrocyte population.These findings underscore the toxic effects of polystyrene nanoparticles on hematopoietic processes,highlighting their potential to compromise organismal health in aquatic environments.
基金supported by the National Research Foundation(NRF)grant funded by the Korean government(NRF-2021K1A3A1A74095929,NRF-2021R1A2C2092375)a Korea Research Council(NRF-2022M3H4A1A0407327112)+1 种基金the intramural grant from the Korea Institute of Science and Technology(Grand Challenge,2Z07012 and Open Research Program,2E33311)Korea National University of Science and Technology grant 2021YS16.
文摘Peptide metabolites are emerging biomolecules with numerous possibilities in biomaterial-based regenerative medicine due to their inherent bioactivities.These small,naturally occurring compounds are intermediates or byproducts of larger proteins and peptides,and they can have profound effects,such as antiviral therapeutics,proangiogenic agents,and regenerative medicinal applications.This study is among the first to focus on using thymosinβ4 protein-derived metabolites to pioneer novel applications for peptide metabolites in biomaterials.This study found that the novel peptide metabolite acetyl-thymosinβ4(amino acid 1-17)(Ac-Tβ_(1-17))exhibited significant protease inhibition activity against SARS-CoV-2,surpassing its precursor protein.Additionally,Ac-Tβ_(1-17) demonstrated beneficial effects,such as cell proliferation,wound healing,and scavenging of reactive oxygen species(ROS)in human umbilical vein endothelial cells(HUVEC).Integrating Ac-Tβ_(1-17) into a peptide-based scaffold facilitated cell growth and angiogenesis inside the scaffold and through gradual release into the surrounding environment.The Ac-Tβ_(1-17) peptide treatment induced significant biochemical responses in HUVEC,increasing Akt,ERK,PI3K,MEK,and Bcl-2 gene expression and proangiogenic proteins.Ac-Tβ_(1-17) peptide treatment showed similar results in ex vivo by enhancing mouse fetal metatarsal growth and angiogenesis.These findings highlight the potential of natural protein metabolites to generate biologically active peptides,offering a novel strategy for enhancing biomaterial compatibility.This approach holds promise for developing therapeutic biomaterials using peptide metabolites,presenting exciting prospects for future research and applications.
