Background The oxidative modification of low-density lipoprotein in theartery wall is currently believed to be central to the pathogenesis of atherosclerosis. Paraoxonase(PON1), an enzyme located on high-density lipop...Background The oxidative modification of low-density lipoprotein in theartery wall is currently believed to be central to the pathogenesis of atherosclerosis. Paraoxonase(PON1), an enzyme located on high-density lipoprotein (HDL) , can prevent low-density lipoprotein(LDL) from oxidation at a certain extent. Recent studies show two other members of paraoxonase genefamily, PON2 and PON3, possess antioxidant properties similar to PON1. The aim of the present studywas to explore the role of PON gene cluster on coronary heart disease (CHD) in Chinese Han women.Methods Seven polymorphisms including PON1 -107C > T, -162G > A, -831G > A, R160G, Q192R, PON2S311C, and PON3 -133C > A were genotyped in 184 female patients with CHD and 239 female controls.The plasma PON1 activity toward phenylacetate was determined in 50 cases and 50 controls randomlyselected. Results The plasma PON1 activities were significantly lower in cases than in controls.Individual SNP analysis showed that cases had significantly higher frequencies of PON1 -107T, -831Gand PON2 311S alleles than controls. The genotype distributions of -107C >T were also significantlydifferent between two groups. The odds ratios for the development of CHD were 1. 66 for -107TCcarriers and 2. 0 for -107TT carriers, compared with -107CC carriers. Haplotype analyses showed thatthe distributions of haplotypes comprised of PON1 -107C > T and PON2 S311C were significantlydifferent between cases and controls, with cases having higher frequency of T-S haplotype (44.8% vs.36.3%, P =0.013). The T-S haplotype remained significantly associated with CHD after adjustingenvironmental risk factors (P = 0.0069). Conclusions This association study suggested that lowerplasma PON1 activity increased the risk of CHD in Chinese woman, which may be mediated by the higherfrequency of -107T allele in cases. Haplotype analyses indicated that there might be somesynergistic effects between the PON1 -107C > T and PON2 S311C polymorphisms.展开更多
Reticulons (RTN) are endoplasmic reticulum- associated protein complexes, which are localized in the endoplasmic reticulum (ER) and identified as markers for neuroendocrine differentiation. At least four different RTN...Reticulons (RTN) are endoplasmic reticulum- associated protein complexes, which are localized in the endoplasmic reticulum (ER) and identified as markers for neuroendocrine differentiation. At least four different RTN genes have been identified in mammals, but in most cases, the functions of the encoded proteins except mammalian RTN4-A and RTN4-B are still elusive. In the present study, mouse reticulon 3 (mRTN3) is cloned and its expression pattern in a variety of tissues is investigated. Three alternatively spliced transcripts of 1.8, 2.8 and 4.2 kb are revealed by Northern blotting hybridization. The 1.8 and 2.8 kb transcripts are expressed in many tissues. The 2.8 kb transcript has a high level in brain and the 4.2 kb transcript is only found in brain. In situ hybridization and immunohistochemical analysis indicated its high expression in non-glial cells in some particular region of mouse central nervous system, such as hippocampus, sub-thalamus nucleus, thalamus nucleus and cerebrum cortex.展开更多
文摘Background The oxidative modification of low-density lipoprotein in theartery wall is currently believed to be central to the pathogenesis of atherosclerosis. Paraoxonase(PON1), an enzyme located on high-density lipoprotein (HDL) , can prevent low-density lipoprotein(LDL) from oxidation at a certain extent. Recent studies show two other members of paraoxonase genefamily, PON2 and PON3, possess antioxidant properties similar to PON1. The aim of the present studywas to explore the role of PON gene cluster on coronary heart disease (CHD) in Chinese Han women.Methods Seven polymorphisms including PON1 -107C > T, -162G > A, -831G > A, R160G, Q192R, PON2S311C, and PON3 -133C > A were genotyped in 184 female patients with CHD and 239 female controls.The plasma PON1 activity toward phenylacetate was determined in 50 cases and 50 controls randomlyselected. Results The plasma PON1 activities were significantly lower in cases than in controls.Individual SNP analysis showed that cases had significantly higher frequencies of PON1 -107T, -831Gand PON2 311S alleles than controls. The genotype distributions of -107C >T were also significantlydifferent between two groups. The odds ratios for the development of CHD were 1. 66 for -107TCcarriers and 2. 0 for -107TT carriers, compared with -107CC carriers. Haplotype analyses showed thatthe distributions of haplotypes comprised of PON1 -107C > T and PON2 S311C were significantlydifferent between cases and controls, with cases having higher frequency of T-S haplotype (44.8% vs.36.3%, P =0.013). The T-S haplotype remained significantly associated with CHD after adjustingenvironmental risk factors (P = 0.0069). Conclusions This association study suggested that lowerplasma PON1 activity increased the risk of CHD in Chinese woman, which may be mediated by the higherfrequency of -107T allele in cases. Haplotype analyses indicated that there might be somesynergistic effects between the PON1 -107C > T and PON2 S311C polymorphisms.
基金This work was supprted by the National Key Basic Rescarch Project(Grant Nos.G1998051002 and 001cb510206)the National High Technology Research and Development Program(Grant Nos.2001AA221041 and 2002BA711A01-03)the National Natural Sciences Foundation of China(Grand No.39830070).
文摘Reticulons (RTN) are endoplasmic reticulum- associated protein complexes, which are localized in the endoplasmic reticulum (ER) and identified as markers for neuroendocrine differentiation. At least four different RTN genes have been identified in mammals, but in most cases, the functions of the encoded proteins except mammalian RTN4-A and RTN4-B are still elusive. In the present study, mouse reticulon 3 (mRTN3) is cloned and its expression pattern in a variety of tissues is investigated. Three alternatively spliced transcripts of 1.8, 2.8 and 4.2 kb are revealed by Northern blotting hybridization. The 1.8 and 2.8 kb transcripts are expressed in many tissues. The 2.8 kb transcript has a high level in brain and the 4.2 kb transcript is only found in brain. In situ hybridization and immunohistochemical analysis indicated its high expression in non-glial cells in some particular region of mouse central nervous system, such as hippocampus, sub-thalamus nucleus, thalamus nucleus and cerebrum cortex.
