Purpose:This study aimed to examine the linkage between gut microbiome taxa and exercise-induced inflammation.Methods:Twenty-five cyclists provided 4 stool samples during a 10-week period and cycled vigorously for 2.25...Purpose:This study aimed to examine the linkage between gut microbiome taxa and exercise-induced inflammation.Methods:Twenty-five cyclists provided 4 stool samples during a 10-week period and cycled vigorously for 2.25 h at 67%maximal oxygen uptake(VO2max)in a laboratory setting.Blood samples were collected pre-and post-exercise,with additional samples collected at 1.5-h,3-h,and 24-h post exercise.Primary outcomes included stool microbiome composition and alpha diversity via whole genome shotgun(WGS)sequencing(averaged from 4 stool samples)and a targeted panel of 75 plasma oxylipins.A total of 5719 taxa were identified,and the 339 that were present in more than 20%of stool samples were used in the analysis.Alpha diversity was calculated by evenness,and the Analysis of Composition of Microbiomes(ANCOM)differential abundance analysis was performed using Quantitative Insights Into Microbial Ecology-2(QIIME2).A composite variable was calculated from 8 pro-inflammatory oxylipins generated from arachidonic acid(ARA)and cytochrome P-450(CYP).Results:ARA-CYP oxylipins were significantly elevated for at least 3-h post-exercise(p<0.001);they were strongly and positively related to Prevotella copri(P.copri)abundance(R2=0.676,p<0.001)and negatively related to gut microbiome alpha diversity(R2=0.771,p<0.001).Conclusion:This analysis revealed for thefirst time a novel,positive relationship between gut microbiome P.copri abundance in cyclists and post-exercise pro-inflammatory oxylipins.These data demonstrate that about two-thirds of the wide variance in inflammation following prolonged and intensive exercise is largely explained by the abundance of a single gut bacterial species:P.copri.展开更多
目的探讨临汾市春季花粉分布特征,明确气象因素对花粉浓度的影响规律。方法应用重力沉降法对临汾市2024年3月1日至6月30日气传花粉进行监测,结合气象站同期数据(平均大气压、温度、湿度、风速、日照时长、降雨量、PM_(10)和PM_(2.5)),...目的探讨临汾市春季花粉分布特征,明确气象因素对花粉浓度的影响规律。方法应用重力沉降法对临汾市2024年3月1日至6月30日气传花粉进行监测,结合气象站同期数据(平均大气压、温度、湿度、风速、日照时长、降雨量、PM_(10)和PM_(2.5)),采用皮尔逊相关性分析气象因素对花粉浓度的影响。结果共收集到花粉总数为16200粒,鉴定到18个种属,以悬铃木属3552粒/1000 mm^(2)(21.93%)、松科油松属3154粒/1000 mm^(2)(19.47%)、桑科2936粒/1000 mm 2(18.12%)花粉为主;平均大气压与花粉浓度之间呈现出显著的正相关(相关系数为0.5243,P<0.01),而日照时长、降雨量、风速、PM_(10)和PM_(2.5)浓度与花粉浓度之间表现出负相关关系,但未达到显著性水平。结论临汾市花粉高峰期出现在3月下旬至4月中旬;气象因素中大气压对花粉浓度具有关键影响;花粉分布特征及其与气象的相关性在最大程度上使花粉症患者精准规避过敏原,为制定区域性花粉症防治策略提供了科学依据。展开更多
基金supported by Ocean Spray (https://www. oceanspray.com/)
文摘Purpose:This study aimed to examine the linkage between gut microbiome taxa and exercise-induced inflammation.Methods:Twenty-five cyclists provided 4 stool samples during a 10-week period and cycled vigorously for 2.25 h at 67%maximal oxygen uptake(VO2max)in a laboratory setting.Blood samples were collected pre-and post-exercise,with additional samples collected at 1.5-h,3-h,and 24-h post exercise.Primary outcomes included stool microbiome composition and alpha diversity via whole genome shotgun(WGS)sequencing(averaged from 4 stool samples)and a targeted panel of 75 plasma oxylipins.A total of 5719 taxa were identified,and the 339 that were present in more than 20%of stool samples were used in the analysis.Alpha diversity was calculated by evenness,and the Analysis of Composition of Microbiomes(ANCOM)differential abundance analysis was performed using Quantitative Insights Into Microbial Ecology-2(QIIME2).A composite variable was calculated from 8 pro-inflammatory oxylipins generated from arachidonic acid(ARA)and cytochrome P-450(CYP).Results:ARA-CYP oxylipins were significantly elevated for at least 3-h post-exercise(p<0.001);they were strongly and positively related to Prevotella copri(P.copri)abundance(R2=0.676,p<0.001)and negatively related to gut microbiome alpha diversity(R2=0.771,p<0.001).Conclusion:This analysis revealed for thefirst time a novel,positive relationship between gut microbiome P.copri abundance in cyclists and post-exercise pro-inflammatory oxylipins.These data demonstrate that about two-thirds of the wide variance in inflammation following prolonged and intensive exercise is largely explained by the abundance of a single gut bacterial species:P.copri.
文摘目的分析抗干燥综合征抗原B(Sjogren’s syndrome antigen B,SSB)抗体阳性同时抗干燥综合征抗原A(Sjogren’s syndrome antigen A,SSA)抗体阴性的原发性干燥综合征(primary Sjogren’s syndrome,pSS)患者的临床特点。方法回顾性分析2016年5月至2022年1月在国家风湿病数据中心(Chinese Rheumatism Data Center,CRDC)注册登记的pSS患者临床资料,比较抗SSB抗体阳性同时抗SSA抗体阴性pSS患者(观察组)与抗SSA抗体阳性(抗SSB抗体阳性或阴性)pSS患者(对照组)的临床特点差异。结果共纳入pSS患者8188例,其中女性7801例,男性387例,中位年龄51(40,59)岁。观察组pSS患者101例(1.2%),对照组8087例(98.8%),两组患者的性别、年龄及病程比较差异均无统计学意义(P均>0.05)。临床特征方面,相较于对照组,观察组出现口干(97.0%比88.5%,P<0.05)、腮腺肿大(31.7%比17.5%,P<0.05)的比例更高,EULAR干燥综合征患者报告指数(European League Against Rheumatism Sjogren’s Syndrome Patient Reported Index,ESSPRI)中ESSPRI干燥评分[50.0(39.3,60.0)比41.0(23.0,60.0),P<0.05]、ESSPRI疲乏评分[50.0(23.8,59.8)比34.0(15.0,52.0),P<0.05]、ESSPRI肢体痛评分[50.0(20.0,55.8)比20.0(0.0,42.0),P<0.05]均更高,医生总体评估(Physician’s Global Assessment,PGA)评分[1.3(0.8,1.6)比1.0(0.5,1.5),P<0.05]也更高,但观察组EULAR干燥综合征疾病活动指数(European League Against Rheumatism Sjogren’s Syndrome Disease Activity Index,ESSDAI)评分低于对照组[2.0(0.0,5.0)比4.0(0.0,9.0),P<0.05]。结论抗SSB抗体阳性同时抗SSA抗体阴性的pSS患者更易出现腮腺肿大和口干。
文摘目的探讨临汾市春季花粉分布特征,明确气象因素对花粉浓度的影响规律。方法应用重力沉降法对临汾市2024年3月1日至6月30日气传花粉进行监测,结合气象站同期数据(平均大气压、温度、湿度、风速、日照时长、降雨量、PM_(10)和PM_(2.5)),采用皮尔逊相关性分析气象因素对花粉浓度的影响。结果共收集到花粉总数为16200粒,鉴定到18个种属,以悬铃木属3552粒/1000 mm^(2)(21.93%)、松科油松属3154粒/1000 mm^(2)(19.47%)、桑科2936粒/1000 mm 2(18.12%)花粉为主;平均大气压与花粉浓度之间呈现出显著的正相关(相关系数为0.5243,P<0.01),而日照时长、降雨量、风速、PM_(10)和PM_(2.5)浓度与花粉浓度之间表现出负相关关系,但未达到显著性水平。结论临汾市花粉高峰期出现在3月下旬至4月中旬;气象因素中大气压对花粉浓度具有关键影响;花粉分布特征及其与气象的相关性在最大程度上使花粉症患者精准规避过敏原,为制定区域性花粉症防治策略提供了科学依据。
