The sterile insect technique(SIT)for controlling the Mediterranean fruit fly,Ceratitis capitata(Wiedemann),requires the mass-rearing of males in bio-facilities.This study summarizes the implementation of a Pre-Filter ...The sterile insect technique(SIT)for controlling the Mediterranean fruit fly,Ceratitis capitata(Wiedemann),requires the mass-rearing of males in bio-facilities.This study summarizes the implementation of a Pre-Filter system in the mass-rearing process of C.capitata at the Moscamed program's El Pino facility in Guatemala.The Pre-Filter selection process as presented here was aimed to increase the longevity of the VIENNA 8 D53-strain males released for pest control.Over a 9-year period(2015–2024),the longevity of these males was systemically monitored,and the impact of the Pre-Filter system on mortality,half-life,and survival rate were analyzed.Results indicated a significant reduction in mortality at 48 h,declining from 16.47%in 2017 to 2.56%in 2024.Concurrently,the half-life of males increased from 79.55 h in 2018 to 85.97 h in 2024.The Pre-Filter system shifted the frequency distribution of half-lives toward longer durations,with a peak observed between 76.14 and 86.14 h,whereas populations without the Pre-Filter exhibited shorter half-lives,peaking between 56.14 and 66.14 h.Comparative analyses with historical data(2005–2024)demonstrated that the Pre-Filter system significantly improved the male longevity,compared to pre-implementation periods during which mortality rates were higher and more variable.Additionally,the results revealed a gradual reduction in longevity variability within the population,indicating a more homogeneous longevity among the mass-reared males.展开更多
The High Biosecurity House of the Mediterranean Fly in Metapa(CABIM3,in its Spanish acronym)is a space within the new Moscamed Mexico facility designed to select favorable traits that mitigate the negative effects of ...The High Biosecurity House of the Mediterranean Fly in Metapa(CABIM3,in its Spanish acronym)is a space within the new Moscamed Mexico facility designed to select favorable traits that mitigate the negative effects of mass production while enhancing male competitiveness,ultimately increasing the efficiency of the sterile insect technique(SIT).The CABIM3 serves as an environmentally enriched space where insects,whose offspring will initiate the mass production process,are confined.In this study,the sexual performance of males and oviposition behavior of females from mass-reared and wild strains of Anastrepha ludens(Loew)and A.obliqua(Macquart)were compared under field cage conditions,inside CABIM3 and in an orchard,to evaluate the suitability of CABIM3's environmental conditions,including light intensity,temperature,and relative humidity.The results revealed significant differences in male sexual performance and female oviposition behavior between the CABIM3 areas and the orchard.Despite these differences,our findings suggest that a breeding facility like CABIM3 could be a valuable tool for improving the attributes of insects used in SIT programs.展开更多
文摘The sterile insect technique(SIT)for controlling the Mediterranean fruit fly,Ceratitis capitata(Wiedemann),requires the mass-rearing of males in bio-facilities.This study summarizes the implementation of a Pre-Filter system in the mass-rearing process of C.capitata at the Moscamed program's El Pino facility in Guatemala.The Pre-Filter selection process as presented here was aimed to increase the longevity of the VIENNA 8 D53-strain males released for pest control.Over a 9-year period(2015–2024),the longevity of these males was systemically monitored,and the impact of the Pre-Filter system on mortality,half-life,and survival rate were analyzed.Results indicated a significant reduction in mortality at 48 h,declining from 16.47%in 2017 to 2.56%in 2024.Concurrently,the half-life of males increased from 79.55 h in 2018 to 85.97 h in 2024.The Pre-Filter system shifted the frequency distribution of half-lives toward longer durations,with a peak observed between 76.14 and 86.14 h,whereas populations without the Pre-Filter exhibited shorter half-lives,peaking between 56.14 and 66.14 h.Comparative analyses with historical data(2005–2024)demonstrated that the Pre-Filter system significantly improved the male longevity,compared to pre-implementation periods during which mortality rates were higher and more variable.Additionally,the results revealed a gradual reduction in longevity variability within the population,indicating a more homogeneous longevity among the mass-reared males.
基金funded through contract D42017 by the International Atomic Energy Agency(IAEA)in collaboration with SENASICADGSV and the Programa Nacional de Moscas de la Fruta.
文摘The High Biosecurity House of the Mediterranean Fly in Metapa(CABIM3,in its Spanish acronym)is a space within the new Moscamed Mexico facility designed to select favorable traits that mitigate the negative effects of mass production while enhancing male competitiveness,ultimately increasing the efficiency of the sterile insect technique(SIT).The CABIM3 serves as an environmentally enriched space where insects,whose offspring will initiate the mass production process,are confined.In this study,the sexual performance of males and oviposition behavior of females from mass-reared and wild strains of Anastrepha ludens(Loew)and A.obliqua(Macquart)were compared under field cage conditions,inside CABIM3 and in an orchard,to evaluate the suitability of CABIM3's environmental conditions,including light intensity,temperature,and relative humidity.The results revealed significant differences in male sexual performance and female oviposition behavior between the CABIM3 areas and the orchard.Despite these differences,our findings suggest that a breeding facility like CABIM3 could be a valuable tool for improving the attributes of insects used in SIT programs.
