In this report we have evaluated metabolite and RNA profiling technologies to begin to understand the natural variation in these biomolecules found in commercial-quality, conventional (non-GM) maize hybrids. Our analy...In this report we have evaluated metabolite and RNA profiling technologies to begin to understand the natural variation in these biomolecules found in commercial-quality, conventional (non-GM) maize hybrids. Our analyses focus on mature grain, the article of commerce that is most typically subjected to the rigorous studies involved in the comparative safety assessment of GM products. We have used a population of conventionally-bred maize hybrids that derive from closely related inbred parents grown under standard field conditions across geographically similar locations. This study highlights the large amount of natural variation in metabolites and transcripts across conventional maize germplasm grown under normal field conditions, and underscores the critical need for further extensive studies before these technologies can be seriously considered for utility in the comparative safety assessment of GM crops.展开更多
Roses,which have been cultivated for at least 5000 years,are one of the most important ornamental crops in the world.Because of the interspecific nature and high heterozygosity in commercial roses,the genetic resource...Roses,which have been cultivated for at least 5000 years,are one of the most important ornamental crops in the world.Because of the interspecific nature and high heterozygosity in commercial roses,the genetic resources available for rose are limited.To effectively identify markers associated with QTL controlling important traits,such as disease resistance,abundant markers along the genome and careful phenotyping are required.Utilizing genotyping by sequencing technology and the strawberry genome(Fragaria vesca v2.0.a1)as a reference,we generated thousands of informative single nucleotide polymorphism(SNP)markers.These SNPs along with known bridge simple sequence repeat(SSR)markers allowed us to create the first high-density integrated consensus map for diploid roses.Individual maps were first created for populations J06-20-14-3דLittle Chief”(J14-3×LC),J06-20-14-3דVineyard Song”(J14-3×VS)and“Old Blush”דRed Fairy”(OB×RF)and these maps were linked with 824 SNPs and 13 SSR bridge markers.The anchor SSR markers were used to determine the numbering of the rose linkage groups.The diploid consensus map has seven linkage groups(LGs),a total length of 892.2 cM,and an average distance of 0.25 cM between 3527 markers.By combining three individual populations,the marker density and the reliability of the marker order in the consensus map was improved over a single population map.Extensive synteny between the strawberry and diploid rose genomes was observed.This consensus map will serve as the tool for the discovery of marker–trait associations in rose breeding using pedigree-based analysis.The high level of conservation observed between the strawberry and rose genomes will help further comparative studies within the Rosaceae family and may aid in the identification of candidate genes within QTL regions.展开更多
A population of common ragweed in Ontario was confirmed to be resistant to glyphosate in 2011. Group 2 [acetolactate synthase (ALS) inhibitors] resistant common ragweed was first confirmed in Ontario in 2000. Previous...A population of common ragweed in Ontario was confirmed to be resistant to glyphosate in 2011. Group 2 [acetolactate synthase (ALS) inhibitors] resistant common ragweed was first confirmed in Ontario in 2000. Previously, glyphosate provided excellent control of common ragweed in glyphosate resistant soybean but with the confirmation of glyphosate resistant (GR) common ragweed, alternative herbicides need to be evaluated. Eight field trials with preplant herbicides were completed over two years (2013 and 2014) in fields with confirmed GR common ragweed. Tank-mixes of glyphosate and linuron or metribuzin provided 88% - 99% and 86% - 98% control 4 weeks after application (WAA) and 80% - 92% and 80% - 95% control 8 WAA, respectively. However, these herbicides also had among the highest environmental impact of the herbicides tested. Based on the results of these studies, GR common ragweed can be controlled with residual herbicides when applied preemergence in soybean. Currently, there are no post emergence herbicides that provide adequate control of GR common ragweed, therefore, preemergence herbicides with residual are essential for full season control.展开更多
Brazil is the world leader in sugarcane production and the largest sugar exporter. Developing new varieties is one of the main factors that contribute to yield increase. In order to select the best genotypes, during t...Brazil is the world leader in sugarcane production and the largest sugar exporter. Developing new varieties is one of the main factors that contribute to yield increase. In order to select the best genotypes, during the final selection stage, varieties are tested in different environments (locations and years), and breeders need to estimate the phenotypic performance for main traits such as tons of cane yield per hectare (TCH) considering the genotype × environment interaction (GEI) effect. Geneticists and biometricians have used different methods and there is no clear consensus of the best method. In this study, we present a comparison of three methods, viz. Eberhart-Russel (ER), additive main effects and multiplicative interaction (AMMI) and mixed model (REML/BLUP), in a simulation study performed in the R computing environment to verify the effectiveness of each method in detecting GEI, and assess the particularities of each method from a statistical standpoint. In total, 63 cases representing different conditions were simulated, generating more than 34 million data points for analysis by each of the three methods. The results show that each method detects GEI differently in a different way, and each has some limitations. All three methods detected GEI effectively, but the mixed model showed higher sensitivity. When applying the GEI analysis, firstly it is important to verify the assumptions inherent in each method and these limitations should be taken into account when choosing the method to be used.展开更多
Giant ragweed (Ambrosia trifida L.) is competitive with agronomic crops and can cause significant yield losses. Rapid adoption of glyphosate-resistant (GR) crops and a concomitant increase in the reliance on glyphosat...Giant ragweed (Ambrosia trifida L.) is competitive with agronomic crops and can cause significant yield losses. Rapid adoption of glyphosate-resistant (GR) crops and a concomitant increase in the reliance on glyphosate for weed management has led to the evolution of GR giant ragweed in Ontario, Canada. Field studies were conducted to evaluate the level of resistance in giant ragweed biotypes from Ontario, and to evaluate the effectiveness of various postemer-gence (POST) herbicides in soybean (Glycine max L.). The effective dose (ED) to provide 50%, 80% and 95% giant ragweed control was up to 1658, 9991 and >43200 g?a.e.?ha–1 4 weeks after application (WAA), respectively. For effective control, growers would need to apply glyphosate 18 times greater than the recommended field application dose. Glyphosate applied at the recommended field dose of 900 g?a.e.?ha–1 provided up to 57% control and resulted in soybean yield equivalent to the weedy check. Cloransulam-methyl applied POST provided up to 99% control, reduced giant ragweed density 98%, reduced giant ragweed shoot dry weight 99% and resulted in soybean yield equivalent to the weedfree check. Chlorimuron-ethyl, fomesafen, imazethapyr and imazethapyr plus bentazon applied alone or with glyphosate did not provide adequate control of GR giant ragweed. Based on these results, some GR giant ragweed biotypes from Ontario have evolved a high level of resistance to glyphosate. Cloransulam-methyl applied POST was the only herbicide that provided adequate control and suggests that additional weed management tactics will need to be implemented in order to effectively manage GR giant ragweed.展开更多
Giant ragweed was the first glyphosate resistant weed identified in Canada. It is a very competetive weed in row crop production and has been found to drastically reduce yields of soybean;therefore, control of this co...Giant ragweed was the first glyphosate resistant weed identified in Canada. It is a very competetive weed in row crop production and has been found to drastically reduce yields of soybean;therefore, control of this competitive weed is essential. The objective of this study was to determine effective control options for glyphosate resistant giant ragweed in soybean with herbicides applied preplant. Eighteen herbicide combinations were evaluated in field studies conducted in 2011 and 2012 at five locations with confirmed glyphosate resistant giant ragweed. Glyphosate plus 2,4-D ester or amitrole provided the best control of glyphosate resistant giant ragweed 4 WAA. Glyphosate plus 2,4-D ester provided 98 to 99% control and was equivalent to the weed free check at all locations. Glyphosate plus amitrole provided 90% to 93% control and was equivalent to the weed free check at 4 of 5 locations. Herbicides providing residual activity provided variable control across all locations. Of the herbicides with residual activity evaluated, glyphosate plus linuron provided the best control of glyphosate resistant giant ragweed;however, control was inconsistent across locations and years. Glyphosate plus linuron provided 23% to 99% controland was equal to the weed free check at one location 8 WAA.展开更多
Informative,portable,and efficient DNA markers have the potential to accelerate genetic gain in cotton breeding.Discovery and widespread application of DNA markers to cotton has
Field experiments were conducted to evaluate the phytotoxicity of flazasulfuron on citrus species and efficacy on weeds when applied alone or in combination with other herbicides. Grapefruit was the most sensitive and...Field experiments were conducted to evaluate the phytotoxicity of flazasulfuron on citrus species and efficacy on weeds when applied alone or in combination with other herbicides. Grapefruit was the most sensitive and tangerine was the least sensitive to flazasulfuron. Injury to grapefruit was 70% with the application of flazasulfuron at 0.20 kg a.i. ha–1 at 60 DAT and was reduced (5%) when flazasulfuron at 0.05 kg a.i. ha–1 was tank mixed with glyphosate at 0.84 kg·a.i.· ha–1. Flazasulfuron alone at all rates did not control grass weeds and common ragweed. Florida/Brazil pusley was moderately controlled with high rates of flazasulfuron from 30 to 45 DAT;however, control did not exceed 75%. There was good control of Spanishneedles (78% - 85%) and horseweed (73% - 81%) with flazasulfuron at all rates at 30 DAT but control declined later in the season. Tank mix of flazasulfuron with glyphosate improved flazasulfuron efficacy on grass and broadleaf weeds. Flazasulfuron at 0.07 kg a.i. ha–1 plus glyphosate at 1.70 kg a.i. ha–1 was more effective in controlling grass weeds (83%) at 60 DAT and provided the highest control (79%) of Florida/Brazil pusley at 45 DAT. There was excellent control (75% to 94%) of Spanishneedles and adequate control of eastern black nightshade and common ragweed with flazasulfuron tank mixed with glyphosate or diuron. This study showed that flazasulfuron alone does not adequately control grasses and some broadleaf weeds in citrus and tank mixing it with glyphosate or diuron improved flazasulfuron’s efficacy. However, injury to grapefruit was observed at rates which effectively controlled weeds. Further studies are needed to determine the most suitable flazasulfuron rate that could be used to manage weeds in grapefruit.展开更多
A genome-wide association study(GWAS)was applied to detect single nucleotide polymorphisms(SNPs)significantly associated with resistance to Heterodera glycines(HG)also known as the soybean cyst nematode(SCN)in the cor...A genome-wide association study(GWAS)was applied to detect single nucleotide polymorphisms(SNPs)significantly associated with resistance to Heterodera glycines(HG)also known as the soybean cyst nematode(SCN)in the core collection of common bean,Phaseolus vulgaris.There were 84,416 SNPs identified in 363 common bean accessions.GWAS identified SNPs on chromosome(Chr)1 that were significantly associated with resistance to HG type 2.5.7.These SNPs were in linkage disequilibrium with a gene cluster orthologous to the three genes at the Rhg1 locus in soybean.A novel signal on Chr 7 was detected and associated with resistance to HG type 1.2.3.5.6.7.Genomic predictions(GPs)for resistance to these two SCN HG types in common bean achieved prediction accuracy of 0.52 and 0.41,respectively.Our study generated a high-quality SNP panel for 363 common bean accessions and demonstrated that both GWAS and GP were effective strategies to understand the genetic architecture of SCN resistance in common bean.展开更多
Nitrogen fertilization is one of the greatest challenges associated with the production of biofuel from corn grain. The objective of this research was to determine the effect of N fertilization on the content and yiel...Nitrogen fertilization is one of the greatest challenges associated with the production of biofuel from corn grain. The objective of this research was to determine the effect of N fertilization on the content and yield of oil, protein, and starch in corn grain. The project was done in Southeast Missouri （USA）, from 2007 to 2009 in a silt loam soil. Corn grain contains 3.8-4.2% oil, 6.7%-8.9% protein, 68.0%-70.4% extractable starch, and 76.0%-77.7% total starch. The total starch yield ranged from 2.8 to 7.8 mg.ha1 whereas the extractable starch varied between 2.5 to 7.1 mg-ha1. As the N rate went up, the oil and starch content of the grain decreased, whereas the protein content and the protein, starch, and oil yields increased, reaching their maximum at the N rate corresponding to 179.0 kg N.ha~. The potential ethanol yield varied between 616.2 and 7,035.1 L-ha1 depending on the method of conversion of the starch into ethanol, the year and the N rate （P 〈 0.0001）. The negative correlation between N fertilization rate and starch content suggested that when farmers add too much N to their soil to increase grain yield, they reduce the starch content in those grains, and consequently the conversion into bioethanol. Therefore, for biofuel production to be beneficial for both farmers and the power plant owners, an agreement needs to be made with regard to the use of fertilizers.展开更多
Glyphosate resistant giant ragweed is an increasing problem in glyphosate resistant cropping systems in southwestern Ontario. The postemergence herbicides registered for use in soybean in Ontario do not provide consis...Glyphosate resistant giant ragweed is an increasing problem in glyphosate resistant cropping systems in southwestern Ontario. The postemergence herbicides registered for use in soybean in Ontario do not provide consistent control of glyphosate resistant giant ragweed. There is limited research on the lowest effective rate of 2,4-D for the control of glyphosate resistant giant ragweed. Consequently, the objectives of this study were a) to determine the efficacy of herbicides applied postemergence for the control of glyphosate resistant giant ragweed in glyphosate resistant soybean, and b) to determine the lowest effective rate of 2,4-D for the control of glyphosate-resistant giant ragweed. Ten postemergence herbicide combinations and seven rates of 2,4-D were evaluated in field studies conducted in 2011 and 2012 at six locations confirmed with glyphosate-resistant giant ragweed. The post emergence herbicides evaluated did not provide acceptable/consistent control. Of the herbicides evaluated, glyphosate plus cloransulam-methyl provided 26% to 70% control 8 WAA of glyphosate resistant giant ragweed, which was the best of the herbicides combinations evaluated. The doses of 2,4-D required to reduce giant ragweed shoot dry weight by 50, 80 and 95% were 142, 310 and 1048 g a.e. ha-1, respectively.展开更多
Diapause is defined as a period of suspended development in insects and other invertebrates during unfavorable environmental conditions. Diapause is commonly confused with term ＂quiescence＂ as both are dormant devel...Diapause is defined as a period of suspended development in insects and other invertebrates during unfavorable environmental conditions. Diapause is commonly confused with term ＂quiescence＂ as both are dormant development stages. Here this paper aimed to review the research work done on different aspects of diapause. Attempt was made to explain definitions of diapause, incidence, stages and termination of diapause, genetic control, factors affecting diapauses, including temperature, photoperiod, moisture and food, etc..展开更多
Background： Despite its variety of potential applications, the wide implementation of infrared technology in cattle production faces technical, environmental and biological challenges similar to other indicators of m...Background： Despite its variety of potential applications, the wide implementation of infrared technology in cattle production faces technical, environmental and biological challenges similar to other indicators of metabolic state. Nine trials, divided into three classes （technological, environmental and biological factors） were conducted to illustrate the influence of these factors on body surface temperature assessed through infrared imaging. Results： Evaluation of technological factors indicated the following： measurements of body temperatures were strongly repeatable when taken within ]0 s; appropriateness of differing infrared camera technologies was influenced by distance to the target; and results were consistent when analysis of thermographs was compared between judges. Evaluation of environmental factors illustrated that wind and debris caused decreases in body surface temperatures without affecting metabolic rate; additionally, body surface temperature increased due to sunlight but returned to baseline values within minutes of shade exposure. Examination/investigation/exploration of animal factors demonstrated that exercise caused an increase in body surface temperature and metabolic rate. Administration of sedative and anti-sedative caused changes on body surface temperature and metabolic rate, and during late pregnancy a foetal thermal imprint was visible through abdominal infrared imaging. Conclusion： The above factors should be considered in order to standardize operational procedures for taking thermographs, thereby optimizing the use of such technology in cattle operations.展开更多
文摘In this report we have evaluated metabolite and RNA profiling technologies to begin to understand the natural variation in these biomolecules found in commercial-quality, conventional (non-GM) maize hybrids. Our analyses focus on mature grain, the article of commerce that is most typically subjected to the rigorous studies involved in the comparative safety assessment of GM products. We have used a population of conventionally-bred maize hybrids that derive from closely related inbred parents grown under standard field conditions across geographically similar locations. This study highlights the large amount of natural variation in metabolites and transcripts across conventional maize germplasm grown under normal field conditions, and underscores the critical need for further extensive studies before these technologies can be seriously considered for utility in the comparative safety assessment of GM crops.
基金This study was partially funded by the Robert E.Basye Endowment in Rose Genetics,the USDA’s National Institute of Food and Agriculture(NIFA)Specialty Crop Research Initiative projects,“RosBREED:Combining Disease Resistance with Horticultural Quality in New Rosaceous Cultivars”and“Combating Rose Rosette Disease:Short Term and Long Term Approaches”and the China Scholarship Council.
文摘Roses,which have been cultivated for at least 5000 years,are one of the most important ornamental crops in the world.Because of the interspecific nature and high heterozygosity in commercial roses,the genetic resources available for rose are limited.To effectively identify markers associated with QTL controlling important traits,such as disease resistance,abundant markers along the genome and careful phenotyping are required.Utilizing genotyping by sequencing technology and the strawberry genome(Fragaria vesca v2.0.a1)as a reference,we generated thousands of informative single nucleotide polymorphism(SNP)markers.These SNPs along with known bridge simple sequence repeat(SSR)markers allowed us to create the first high-density integrated consensus map for diploid roses.Individual maps were first created for populations J06-20-14-3דLittle Chief”(J14-3×LC),J06-20-14-3דVineyard Song”(J14-3×VS)and“Old Blush”דRed Fairy”(OB×RF)and these maps were linked with 824 SNPs and 13 SSR bridge markers.The anchor SSR markers were used to determine the numbering of the rose linkage groups.The diploid consensus map has seven linkage groups(LGs),a total length of 892.2 cM,and an average distance of 0.25 cM between 3527 markers.By combining three individual populations,the marker density and the reliability of the marker order in the consensus map was improved over a single population map.Extensive synteny between the strawberry and diploid rose genomes was observed.This consensus map will serve as the tool for the discovery of marker–trait associations in rose breeding using pedigree-based analysis.The high level of conservation observed between the strawberry and rose genomes will help further comparative studies within the Rosaceae family and may aid in the identification of candidate genes within QTL regions.
文摘A population of common ragweed in Ontario was confirmed to be resistant to glyphosate in 2011. Group 2 [acetolactate synthase (ALS) inhibitors] resistant common ragweed was first confirmed in Ontario in 2000. Previously, glyphosate provided excellent control of common ragweed in glyphosate resistant soybean but with the confirmation of glyphosate resistant (GR) common ragweed, alternative herbicides need to be evaluated. Eight field trials with preplant herbicides were completed over two years (2013 and 2014) in fields with confirmed GR common ragweed. Tank-mixes of glyphosate and linuron or metribuzin provided 88% - 99% and 86% - 98% control 4 weeks after application (WAA) and 80% - 92% and 80% - 95% control 8 WAA, respectively. However, these herbicides also had among the highest environmental impact of the herbicides tested. Based on the results of these studies, GR common ragweed can be controlled with residual herbicides when applied preemergence in soybean. Currently, there are no post emergence herbicides that provide adequate control of GR common ragweed, therefore, preemergence herbicides with residual are essential for full season control.
文摘Brazil is the world leader in sugarcane production and the largest sugar exporter. Developing new varieties is one of the main factors that contribute to yield increase. In order to select the best genotypes, during the final selection stage, varieties are tested in different environments (locations and years), and breeders need to estimate the phenotypic performance for main traits such as tons of cane yield per hectare (TCH) considering the genotype × environment interaction (GEI) effect. Geneticists and biometricians have used different methods and there is no clear consensus of the best method. In this study, we present a comparison of three methods, viz. Eberhart-Russel (ER), additive main effects and multiplicative interaction (AMMI) and mixed model (REML/BLUP), in a simulation study performed in the R computing environment to verify the effectiveness of each method in detecting GEI, and assess the particularities of each method from a statistical standpoint. In total, 63 cases representing different conditions were simulated, generating more than 34 million data points for analysis by each of the three methods. The results show that each method detects GEI differently in a different way, and each has some limitations. All three methods detected GEI effectively, but the mixed model showed higher sensitivity. When applying the GEI analysis, firstly it is important to verify the assumptions inherent in each method and these limitations should be taken into account when choosing the method to be used.
基金Funding for this project was provided in part by Monsanto Canada Inc.,the Grain Farmers of Ontario and the Agricultural Adaptation Council through the Canadian Agricultural Adaptation Program.
文摘Giant ragweed (Ambrosia trifida L.) is competitive with agronomic crops and can cause significant yield losses. Rapid adoption of glyphosate-resistant (GR) crops and a concomitant increase in the reliance on glyphosate for weed management has led to the evolution of GR giant ragweed in Ontario, Canada. Field studies were conducted to evaluate the level of resistance in giant ragweed biotypes from Ontario, and to evaluate the effectiveness of various postemer-gence (POST) herbicides in soybean (Glycine max L.). The effective dose (ED) to provide 50%, 80% and 95% giant ragweed control was up to 1658, 9991 and >43200 g?a.e.?ha–1 4 weeks after application (WAA), respectively. For effective control, growers would need to apply glyphosate 18 times greater than the recommended field application dose. Glyphosate applied at the recommended field dose of 900 g?a.e.?ha–1 provided up to 57% control and resulted in soybean yield equivalent to the weedy check. Cloransulam-methyl applied POST provided up to 99% control, reduced giant ragweed density 98%, reduced giant ragweed shoot dry weight 99% and resulted in soybean yield equivalent to the weedfree check. Chlorimuron-ethyl, fomesafen, imazethapyr and imazethapyr plus bentazon applied alone or with glyphosate did not provide adequate control of GR giant ragweed. Based on these results, some GR giant ragweed biotypes from Ontario have evolved a high level of resistance to glyphosate. Cloransulam-methyl applied POST was the only herbicide that provided adequate control and suggests that additional weed management tactics will need to be implemented in order to effectively manage GR giant ragweed.
基金Funding for this project was provided in part by Monsanto Canada Inc.the Grain Farmers of Ontario and the Agricultural Adaptation Council through the Canadian Agricultural Adaptation Program.
文摘Giant ragweed was the first glyphosate resistant weed identified in Canada. It is a very competetive weed in row crop production and has been found to drastically reduce yields of soybean;therefore, control of this competitive weed is essential. The objective of this study was to determine effective control options for glyphosate resistant giant ragweed in soybean with herbicides applied preplant. Eighteen herbicide combinations were evaluated in field studies conducted in 2011 and 2012 at five locations with confirmed glyphosate resistant giant ragweed. Glyphosate plus 2,4-D ester or amitrole provided the best control of glyphosate resistant giant ragweed 4 WAA. Glyphosate plus 2,4-D ester provided 98 to 99% control and was equivalent to the weed free check at all locations. Glyphosate plus amitrole provided 90% to 93% control and was equivalent to the weed free check at 4 of 5 locations. Herbicides providing residual activity provided variable control across all locations. Of the herbicides with residual activity evaluated, glyphosate plus linuron provided the best control of glyphosate resistant giant ragweed;however, control was inconsistent across locations and years. Glyphosate plus linuron provided 23% to 99% controland was equal to the weed free check at one location 8 WAA.
文摘Informative,portable,and efficient DNA markers have the potential to accelerate genetic gain in cotton breeding.Discovery and widespread application of DNA markers to cotton has
文摘Field experiments were conducted to evaluate the phytotoxicity of flazasulfuron on citrus species and efficacy on weeds when applied alone or in combination with other herbicides. Grapefruit was the most sensitive and tangerine was the least sensitive to flazasulfuron. Injury to grapefruit was 70% with the application of flazasulfuron at 0.20 kg a.i. ha–1 at 60 DAT and was reduced (5%) when flazasulfuron at 0.05 kg a.i. ha–1 was tank mixed with glyphosate at 0.84 kg·a.i.· ha–1. Flazasulfuron alone at all rates did not control grass weeds and common ragweed. Florida/Brazil pusley was moderately controlled with high rates of flazasulfuron from 30 to 45 DAT;however, control did not exceed 75%. There was good control of Spanishneedles (78% - 85%) and horseweed (73% - 81%) with flazasulfuron at all rates at 30 DAT but control declined later in the season. Tank mix of flazasulfuron with glyphosate improved flazasulfuron efficacy on grass and broadleaf weeds. Flazasulfuron at 0.07 kg a.i. ha–1 plus glyphosate at 1.70 kg a.i. ha–1 was more effective in controlling grass weeds (83%) at 60 DAT and provided the highest control (79%) of Florida/Brazil pusley at 45 DAT. There was excellent control (75% to 94%) of Spanishneedles and adequate control of eastern black nightshade and common ragweed with flazasulfuron tank mixed with glyphosate or diuron. This study showed that flazasulfuron alone does not adequately control grasses and some broadleaf weeds in citrus and tank mixing it with glyphosate or diuron improved flazasulfuron’s efficacy. However, injury to grapefruit was observed at rates which effectively controlled weeds. Further studies are needed to determine the most suitable flazasulfuron rate that could be used to manage weeds in grapefruit.
文摘A genome-wide association study(GWAS)was applied to detect single nucleotide polymorphisms(SNPs)significantly associated with resistance to Heterodera glycines(HG)also known as the soybean cyst nematode(SCN)in the core collection of common bean,Phaseolus vulgaris.There were 84,416 SNPs identified in 363 common bean accessions.GWAS identified SNPs on chromosome(Chr)1 that were significantly associated with resistance to HG type 2.5.7.These SNPs were in linkage disequilibrium with a gene cluster orthologous to the three genes at the Rhg1 locus in soybean.A novel signal on Chr 7 was detected and associated with resistance to HG type 1.2.3.5.6.7.Genomic predictions(GPs)for resistance to these two SCN HG types in common bean achieved prediction accuracy of 0.52 and 0.41,respectively.Our study generated a high-quality SNP panel for 363 common bean accessions and demonstrated that both GWAS and GP were effective strategies to understand the genetic architecture of SCN resistance in common bean.
文摘Nitrogen fertilization is one of the greatest challenges associated with the production of biofuel from corn grain. The objective of this research was to determine the effect of N fertilization on the content and yield of oil, protein, and starch in corn grain. The project was done in Southeast Missouri （USA）, from 2007 to 2009 in a silt loam soil. Corn grain contains 3.8-4.2% oil, 6.7%-8.9% protein, 68.0%-70.4% extractable starch, and 76.0%-77.7% total starch. The total starch yield ranged from 2.8 to 7.8 mg.ha1 whereas the extractable starch varied between 2.5 to 7.1 mg-ha1. As the N rate went up, the oil and starch content of the grain decreased, whereas the protein content and the protein, starch, and oil yields increased, reaching their maximum at the N rate corresponding to 179.0 kg N.ha~. The potential ethanol yield varied between 616.2 and 7,035.1 L-ha1 depending on the method of conversion of the starch into ethanol, the year and the N rate （P 〈 0.0001）. The negative correlation between N fertilization rate and starch content suggested that when farmers add too much N to their soil to increase grain yield, they reduce the starch content in those grains, and consequently the conversion into bioethanol. Therefore, for biofuel production to be beneficial for both farmers and the power plant owners, an agreement needs to be made with regard to the use of fertilizers.
基金Funding for this project was provided in part by Monsanto Canada Inc.,the Grain Farmers of Ontario and the Agricultural Adaptation Council through the Canadian Agricultural Adaptation Program.
文摘Glyphosate resistant giant ragweed is an increasing problem in glyphosate resistant cropping systems in southwestern Ontario. The postemergence herbicides registered for use in soybean in Ontario do not provide consistent control of glyphosate resistant giant ragweed. There is limited research on the lowest effective rate of 2,4-D for the control of glyphosate resistant giant ragweed. Consequently, the objectives of this study were a) to determine the efficacy of herbicides applied postemergence for the control of glyphosate resistant giant ragweed in glyphosate resistant soybean, and b) to determine the lowest effective rate of 2,4-D for the control of glyphosate-resistant giant ragweed. Ten postemergence herbicide combinations and seven rates of 2,4-D were evaluated in field studies conducted in 2011 and 2012 at six locations confirmed with glyphosate-resistant giant ragweed. The post emergence herbicides evaluated did not provide acceptable/consistent control. Of the herbicides evaluated, glyphosate plus cloransulam-methyl provided 26% to 70% control 8 WAA of glyphosate resistant giant ragweed, which was the best of the herbicides combinations evaluated. The doses of 2,4-D required to reduce giant ragweed shoot dry weight by 50, 80 and 95% were 142, 310 and 1048 g a.e. ha-1, respectively.
文摘Diapause is defined as a period of suspended development in insects and other invertebrates during unfavorable environmental conditions. Diapause is commonly confused with term ＂quiescence＂ as both are dormant development stages. Here this paper aimed to review the research work done on different aspects of diapause. Attempt was made to explain definitions of diapause, incidence, stages and termination of diapause, genetic control, factors affecting diapauses, including temperature, photoperiod, moisture and food, etc..
基金the Beef Producers of Ontario,Ontario Ministry of Agriculture and Rural Affairs,Beef Cattle Research Council and Agri-Food Canada for financial support
文摘Background： Despite its variety of potential applications, the wide implementation of infrared technology in cattle production faces technical, environmental and biological challenges similar to other indicators of metabolic state. Nine trials, divided into three classes （technological, environmental and biological factors） were conducted to illustrate the influence of these factors on body surface temperature assessed through infrared imaging. Results： Evaluation of technological factors indicated the following： measurements of body temperatures were strongly repeatable when taken within ]0 s; appropriateness of differing infrared camera technologies was influenced by distance to the target; and results were consistent when analysis of thermographs was compared between judges. Evaluation of environmental factors illustrated that wind and debris caused decreases in body surface temperatures without affecting metabolic rate; additionally, body surface temperature increased due to sunlight but returned to baseline values within minutes of shade exposure. Examination/investigation/exploration of animal factors demonstrated that exercise caused an increase in body surface temperature and metabolic rate. Administration of sedative and anti-sedative caused changes on body surface temperature and metabolic rate, and during late pregnancy a foetal thermal imprint was visible through abdominal infrared imaging. Conclusion： The above factors should be considered in order to standardize operational procedures for taking thermographs, thereby optimizing the use of such technology in cattle operations.
