The interaction of nelin, a cardiac-specific ex- pressed protein of human novel gene nelin, with F-actin was studied by both F-actin cosedimentation in vitro and colocal- ization assays. The results showed that nelin ...The interaction of nelin, a cardiac-specific ex- pressed protein of human novel gene nelin, with F-actin was studied by both F-actin cosedimentation in vitro and colocal- ization assays. The results showed that nelin is a new F-actin binding protein and is colocolized with F-actin in cytoplasm of cells. Three new nelin binding proteins, filamin C subtype, titin N2B subtype and inter-alpha trypsin inhibitor heavy chain precursor (ITIH), were identified from human heart cDNA library using yeast two-hybrid screening. The binding activity of filamin C with nelin was confirmed by coim- munoprecipitation. Filamin C binds to nelin through its C-terminal region. It is indicated that nelin is a cytoskeleton associated protein and acts as a membrane-cytoskeleton as- sociated protein involved in the formation of focal adhesions.展开更多
Epstein-Barr virus (EBV) encoded latent membrane protein 1 (LMP1) is considered as the major oncogenic protein of EBV encoded proteins, which could transactivate many transcription factors including activator protein ...Epstein-Barr virus (EBV) encoded latent membrane protein 1 (LMP1) is considered as the major oncogenic protein of EBV encoded proteins, which could transactivate many transcription factors including activator protein 1 (AP-1). Transcription factor plays its role in biological effects through binding to the target gene promoter,transactivating the transcription of target gene, regulating the target gene expression, etc. Recently, we found that LMP1 could mediate a new heterodimer form of c-Jun and JunB, which could bind to AP1 DNA sequence. In this report,we confirmed p16 as a putative target gene of c-Jun/JunB using bioinformatics. We used Tet-on-LMP1 HNE2 cell line as a cell model, which is a dual-stable LMP1 integrated HNE2 cell line and the expression of LMP1 could be regu-lated by the Tet system, and we wanted to explore whether c-Jun/JunB heterodimers mediated by LMP1 could regulate p16. Data demonstrated that c-Jun/Jun B heterodimers me-diated by LMP1 downregulated both the promoter activity and p16 expression, and accelerated the cell cycle progression. These findings established a new direct connection between the AP-I singnal pathway and the cell cycle, and provided a new model for the carcinogenesis mechanism.展开更多
文摘The interaction of nelin, a cardiac-specific ex- pressed protein of human novel gene nelin, with F-actin was studied by both F-actin cosedimentation in vitro and colocal- ization assays. The results showed that nelin is a new F-actin binding protein and is colocolized with F-actin in cytoplasm of cells. Three new nelin binding proteins, filamin C subtype, titin N2B subtype and inter-alpha trypsin inhibitor heavy chain precursor (ITIH), were identified from human heart cDNA library using yeast two-hybrid screening. The binding activity of filamin C with nelin was confirmed by coim- munoprecipitation. Filamin C binds to nelin through its C-terminal region. It is indicated that nelin is a cytoskeleton associated protein and acts as a membrane-cytoskeleton as- sociated protein involved in the formation of focal adhesions.
文摘Epstein-Barr virus (EBV) encoded latent membrane protein 1 (LMP1) is considered as the major oncogenic protein of EBV encoded proteins, which could transactivate many transcription factors including activator protein 1 (AP-1). Transcription factor plays its role in biological effects through binding to the target gene promoter,transactivating the transcription of target gene, regulating the target gene expression, etc. Recently, we found that LMP1 could mediate a new heterodimer form of c-Jun and JunB, which could bind to AP1 DNA sequence. In this report,we confirmed p16 as a putative target gene of c-Jun/JunB using bioinformatics. We used Tet-on-LMP1 HNE2 cell line as a cell model, which is a dual-stable LMP1 integrated HNE2 cell line and the expression of LMP1 could be regu-lated by the Tet system, and we wanted to explore whether c-Jun/JunB heterodimers mediated by LMP1 could regulate p16. Data demonstrated that c-Jun/Jun B heterodimers me-diated by LMP1 downregulated both the promoter activity and p16 expression, and accelerated the cell cycle progression. These findings established a new direct connection between the AP-I singnal pathway and the cell cycle, and provided a new model for the carcinogenesis mechanism.
