Cadmium-metallothionein （Cd-MT） of Pteria penguin was used to immunize domestic rabbit in order to obtain polyclonal antibody against Cd-MT. Using the anti-Cd-MT antibody, a method of indirect non-competitive enzyme...Cadmium-metallothionein （Cd-MT） of Pteria penguin was used to immunize domestic rabbit in order to obtain polyclonal antibody against Cd-MT. Using the anti-Cd-MT antibody, a method of indirect non-competitive enzyme-linked immunosorbent assay （non-competitive ELISA） was established to detect shellfish Cd-MT. In this case, the minimum detectable concentration of P. penguin Cd-MT was （4.563±0.051） ng/mL. The linear range of detection was 9.75-2.0×104 ng/mL. Intra-assay relative standard deviation （RSD） was less than 11.6%, and inter-assay RSD less than 6.7%. The recovery rates ranged from 83.7% to 119.0%. The polyclonal antibody against Cd-MT of P. penguin can also be used to detect MT of other four types of shellfish. In the crude extracts from different organs of P. penguin, the MT concentrations determined by this method matched well with the concentrations of cadmium detected by the atomic absorption spectroscopy.展开更多
基金Supported by the Earmarked Fund for Modern Agro-Industry Technology Research System (CARS-48-07B)Guangdong Provincial Agriculture Key Project (2010B020313005)
文摘Cadmium-metallothionein （Cd-MT） of Pteria penguin was used to immunize domestic rabbit in order to obtain polyclonal antibody against Cd-MT. Using the anti-Cd-MT antibody, a method of indirect non-competitive enzyme-linked immunosorbent assay （non-competitive ELISA） was established to detect shellfish Cd-MT. In this case, the minimum detectable concentration of P. penguin Cd-MT was （4.563±0.051） ng/mL. The linear range of detection was 9.75-2.0×104 ng/mL. Intra-assay relative standard deviation （RSD） was less than 11.6%, and inter-assay RSD less than 6.7%. The recovery rates ranged from 83.7% to 119.0%. The polyclonal antibody against Cd-MT of P. penguin can also be used to detect MT of other four types of shellfish. In the crude extracts from different organs of P. penguin, the MT concentrations determined by this method matched well with the concentrations of cadmium detected by the atomic absorption spectroscopy.
