AIM： To compare the corneal biomechanical outcomes at one year after laser in situ keratomileusis（LASIK） with the flaps created by Ziemer and Moria M2 microkeratome with 110 head and -20 blade.METHODS： Totally 100...AIM： To compare the corneal biomechanical outcomes at one year after laser in situ keratomileusis（LASIK） with the flaps created by Ziemer and Moria M2 microkeratome with 110 head and -20 blade.METHODS： Totally 100 eyes of 50 consecutive patients were enrolled in this prospective study and divided into two groups for corneal flaps created by Ziemer Femto LDV and Moria M2 microkeratome with 110 head and -20 blade.Corneal biomechanical properties including cornea resistance factor（CRF） and cornea hysteresis（CH） were measured before and 1,3,6,12 mo after surgery by ocular response analyzer.Central cornea thickness and corneal flap thickness were measured by optical coherence tomography.RESULTS： The ablation depth（P=0.693）,residual corneal thickness（P=0.453）,and postoperative corneal curvature（P=0.264） were not significant different between Ziemer group and Moria 110-20 group after surgery.The residual stromal bed thickness,corneal flap thickness,CH and CRF at 12 mo after surgery were significant different between Ziemer group and Moria 110-20 group（P〈0.01）; Ziemer group gained better corneal biomechanical results.The CRF and CH increased gradually from 1 to12 mo after surgery in Ziemer group,increased from 1 to 6 mo but decreased from 6 to 12 mo in Moria 110-20 group.Both CRF and CH at one year after surgery increased with the increasing of residual cornea thickness; pre-LASIK CRF,CRF also increased with residual stromal bed thickness,while CH decreased with the increasing of pre-LASIK intraocular pressure and cornea flap thickness（P〈0.01）.CONCLUSION： In one year follow-up,femtosecond laser can provide better cornea flaps with stable cornea biomechanics than mechanical microkeratome.展开更多
The effects of E-cadherin-transfected neural stem cells（NSCs） transplantation for spinal cord injury（SCI） in rats were investigated. Sixty SD rats were randomly divided into model control group, NSCs group, empty ...The effects of E-cadherin-transfected neural stem cells（NSCs） transplantation for spinal cord injury（SCI） in rats were investigated. Sixty SD rats were randomly divided into model control group, NSCs group, empty plasmid group and E-cadherin overexpression group（n=15 each）. The animal SCI model was established by using the modified Allen＇s method. NSCs were cultured. Rats in NSCs group were subjected to NSCs transplantation. E-cadherin gene eucaryotic expression vector and pcDNA3.1-E-cadherin were respectively transfected into cultured NSCs, serving as empty plasmid group and E-cadherin overexpression group respectively. At 7th day after transplantation, neurological function of all rats was assessed by Tarlov score. After rats were sacrificed in each group, the number of BrdU and Nestin positive cells was counted by immunohistochemistry. Immumofluorescence method was used to detect the expression of neurofilament protein（NF） and glial fibrillary acidic protein（GFAP）. As compared with model control group, the Tarlov score and the number of of BrdU and Nestin positive cells, and the expression of NF and GFAP in NSCs group, empty plasmid group, and E-cadherin overexpression group were increased significantly（P〈0.05）, and those in the E-cadherin overexpression group were increased more significantly than the other transplantation groups（P〈0.05）. It was suggested that E-cadherin could be conductive to nerve regeneration and repair probably by promoting the proliferation and differentiation of NSCs.展开更多
Objective: To observe the therapeutic effects of different acupuncture methods for spastic hemiparalysis due to cerebrovascular disorders. Methods: 90 cases of spastic hemiparalysis after wind-stroke were randomly div...Objective: To observe the therapeutic effects of different acupuncture methods for spastic hemiparalysis due to cerebrovascular disorders. Methods: 90 cases of spastic hemiparalysis after wind-stroke were randomly divided into a control group of 30 cases, a body acupuncture group of 30 cases and a point-penetrating acupuncture group of 30 cases. All the patients were given 4 courses of treatment, with 7 sessions constituting one therapeutic course and with a one-day interval between courses. The evaluations were made 3 times, i.e. once before treatment and once every 15 days during the treatment. Results: The therapeutic effects in the point-penetrating acupuncture group were obviously superior to those of both the control group and the body acupuncture group. Conclusion: The point-penetrating acupuncture is an effective therapy for treating spastic hemiparalysis due to cerebrovascular disorders.展开更多
To observe the effects of Jieyu Anshen Decoction combined with Estazolam tablet on sleep index and neurotransmitter in the brain of insomnia patients. Methods: A total of 100 insomnia patients from February 2019 to Ja...To observe the effects of Jieyu Anshen Decoction combined with Estazolam tablet on sleep index and neurotransmitter in the brain of insomnia patients. Methods: A total of 100 insomnia patients from February 2019 to January 2021 were selected as subjects of this study and divided into 2 groups with 50 patients in each group. The control group was treated with Esazolam tablets, and the experimental group was treated with Jieyu Anshen Decoction. The sleep condition and the level of neurotransmitters in the brain were compared between the two groups. Results: After treatment, sleep efficiency, sleep time, sleep quality and Pittsburgh total score of experimental group were significantly better than those of control group (P 0.05). The level of GABA, DA and 5-HT was better than that of control group, the total effective rate was higher than that of control group, and the incidence of adverse reactions was lower than that of control group (P 0.05). Conclusion: Jieyu Anshen Decoction combined with Estazolam tablet has significant therapeutic effect on insomnia patients, and can effectively improve the clinical symptoms of patients.展开更多
Objective:To observe the clinical efficacy of combined acupuncture and medication therapy in treating cerebral infarction in the acute stage and its impact on cerebral blood flow.Methods:A total of 160 patients with c...Objective:To observe the clinical efficacy of combined acupuncture and medication therapy in treating cerebral infarction in the acute stage and its impact on cerebral blood flow.Methods:A total of 160 patients with cerebral infarction were divided into 4 groups using the random number table method,with 40 cases in each group.Conventional treatment was given to all groups.Besides,the Chinese medication group received Tong Shen Fu Nao Wan;the acupuncture group received Xing Nao Kai Qiao(mind-refreshing and orifice-opening)needling therapy;the acupuncture-medication group received both Tong Shen Fu Nao Wan and Xing Nao Kai Qiao needling therapy.After 4 weeks of treatment,the clinical efficacy was assessed,and changes in the traditional Chinese medicine(TCM)symptom score and hemodynamics were compared among the four groups,as well as complications and adverse reactions.Results:After treatment,the acupuncture-medication group had a higher total effective rate compared to the other three groups(P<0.05).After the intervention,the National Institutes of Health stroke scale(NIHSS)score dropped in all groups(P<0.05)and was lower in the acupuncture-medication group than in the other three groups(P<0.05);all groups showed a decrease in the TCM symptom score(P<0.05),and the acupuncture-medication group was significantly lower than the other groups(P<0.05).After treatment,the blood flow velocity of the bilateral anterior cerebral artery(ACA),middle cerebral artery(MCA),and posterior cerebral artery(PCA)increased in every group(P<0.05),and the acupuncture-medication group had higher blood flow velocities compared to the other three groups(P<0.05).The acupuncture-medication group had the fewest number of complications among the four groups(P<0.05),and there was no statistical significance when comparing the adverse reactions among the groups(P>0.05).Conclusion:Compared to the separate use,the combined use of acupuncture and medication enhances the clinical efficacy,accelerates cerebral blood circulation,and boosts the recovery in treating cerebral infarction.展开更多
文摘AIM： To compare the corneal biomechanical outcomes at one year after laser in situ keratomileusis（LASIK） with the flaps created by Ziemer and Moria M2 microkeratome with 110 head and -20 blade.METHODS： Totally 100 eyes of 50 consecutive patients were enrolled in this prospective study and divided into two groups for corneal flaps created by Ziemer Femto LDV and Moria M2 microkeratome with 110 head and -20 blade.Corneal biomechanical properties including cornea resistance factor（CRF） and cornea hysteresis（CH） were measured before and 1,3,6,12 mo after surgery by ocular response analyzer.Central cornea thickness and corneal flap thickness were measured by optical coherence tomography.RESULTS： The ablation depth（P=0.693）,residual corneal thickness（P=0.453）,and postoperative corneal curvature（P=0.264） were not significant different between Ziemer group and Moria 110-20 group after surgery.The residual stromal bed thickness,corneal flap thickness,CH and CRF at 12 mo after surgery were significant different between Ziemer group and Moria 110-20 group（P〈0.01）; Ziemer group gained better corneal biomechanical results.The CRF and CH increased gradually from 1 to12 mo after surgery in Ziemer group,increased from 1 to 6 mo but decreased from 6 to 12 mo in Moria 110-20 group.Both CRF and CH at one year after surgery increased with the increasing of residual cornea thickness; pre-LASIK CRF,CRF also increased with residual stromal bed thickness,while CH decreased with the increasing of pre-LASIK intraocular pressure and cornea flap thickness（P〈0.01）.CONCLUSION： In one year follow-up,femtosecond laser can provide better cornea flaps with stable cornea biomechanics than mechanical microkeratome.
文摘The effects of E-cadherin-transfected neural stem cells（NSCs） transplantation for spinal cord injury（SCI） in rats were investigated. Sixty SD rats were randomly divided into model control group, NSCs group, empty plasmid group and E-cadherin overexpression group（n=15 each）. The animal SCI model was established by using the modified Allen＇s method. NSCs were cultured. Rats in NSCs group were subjected to NSCs transplantation. E-cadherin gene eucaryotic expression vector and pcDNA3.1-E-cadherin were respectively transfected into cultured NSCs, serving as empty plasmid group and E-cadherin overexpression group respectively. At 7th day after transplantation, neurological function of all rats was assessed by Tarlov score. After rats were sacrificed in each group, the number of BrdU and Nestin positive cells was counted by immunohistochemistry. Immumofluorescence method was used to detect the expression of neurofilament protein（NF） and glial fibrillary acidic protein（GFAP）. As compared with model control group, the Tarlov score and the number of of BrdU and Nestin positive cells, and the expression of NF and GFAP in NSCs group, empty plasmid group, and E-cadherin overexpression group were increased significantly（P〈0.05）, and those in the E-cadherin overexpression group were increased more significantly than the other transplantation groups（P〈0.05）. It was suggested that E-cadherin could be conductive to nerve regeneration and repair probably by promoting the proliferation and differentiation of NSCs.
文摘Objective: To observe the therapeutic effects of different acupuncture methods for spastic hemiparalysis due to cerebrovascular disorders. Methods: 90 cases of spastic hemiparalysis after wind-stroke were randomly divided into a control group of 30 cases, a body acupuncture group of 30 cases and a point-penetrating acupuncture group of 30 cases. All the patients were given 4 courses of treatment, with 7 sessions constituting one therapeutic course and with a one-day interval between courses. The evaluations were made 3 times, i.e. once before treatment and once every 15 days during the treatment. Results: The therapeutic effects in the point-penetrating acupuncture group were obviously superior to those of both the control group and the body acupuncture group. Conclusion: The point-penetrating acupuncture is an effective therapy for treating spastic hemiparalysis due to cerebrovascular disorders.
文摘To observe the effects of Jieyu Anshen Decoction combined with Estazolam tablet on sleep index and neurotransmitter in the brain of insomnia patients. Methods: A total of 100 insomnia patients from February 2019 to January 2021 were selected as subjects of this study and divided into 2 groups with 50 patients in each group. The control group was treated with Esazolam tablets, and the experimental group was treated with Jieyu Anshen Decoction. The sleep condition and the level of neurotransmitters in the brain were compared between the two groups. Results: After treatment, sleep efficiency, sleep time, sleep quality and Pittsburgh total score of experimental group were significantly better than those of control group (P 0.05). The level of GABA, DA and 5-HT was better than that of control group, the total effective rate was higher than that of control group, and the incidence of adverse reactions was lower than that of control group (P 0.05). Conclusion: Jieyu Anshen Decoction combined with Estazolam tablet has significant therapeutic effect on insomnia patients, and can effectively improve the clinical symptoms of patients.
文摘Objective:To observe the clinical efficacy of combined acupuncture and medication therapy in treating cerebral infarction in the acute stage and its impact on cerebral blood flow.Methods:A total of 160 patients with cerebral infarction were divided into 4 groups using the random number table method,with 40 cases in each group.Conventional treatment was given to all groups.Besides,the Chinese medication group received Tong Shen Fu Nao Wan;the acupuncture group received Xing Nao Kai Qiao(mind-refreshing and orifice-opening)needling therapy;the acupuncture-medication group received both Tong Shen Fu Nao Wan and Xing Nao Kai Qiao needling therapy.After 4 weeks of treatment,the clinical efficacy was assessed,and changes in the traditional Chinese medicine(TCM)symptom score and hemodynamics were compared among the four groups,as well as complications and adverse reactions.Results:After treatment,the acupuncture-medication group had a higher total effective rate compared to the other three groups(P<0.05).After the intervention,the National Institutes of Health stroke scale(NIHSS)score dropped in all groups(P<0.05)and was lower in the acupuncture-medication group than in the other three groups(P<0.05);all groups showed a decrease in the TCM symptom score(P<0.05),and the acupuncture-medication group was significantly lower than the other groups(P<0.05).After treatment,the blood flow velocity of the bilateral anterior cerebral artery(ACA),middle cerebral artery(MCA),and posterior cerebral artery(PCA)increased in every group(P<0.05),and the acupuncture-medication group had higher blood flow velocities compared to the other three groups(P<0.05).The acupuncture-medication group had the fewest number of complications among the four groups(P<0.05),and there was no statistical significance when comparing the adverse reactions among the groups(P>0.05).Conclusion:Compared to the separate use,the combined use of acupuncture and medication enhances the clinical efficacy,accelerates cerebral blood circulation,and boosts the recovery in treating cerebral infarction.
