Spatial survey of zooplankton community composition within the upper 10 m layer in Lake Kinneret was carried out. Samples were collected by plankton net (63 μ mesh size) and counted organisms were sorted by 2 groups:...Spatial survey of zooplankton community composition within the upper 10 m layer in Lake Kinneret was carried out. Samples were collected by plankton net (63 μ mesh size) and counted organisms were sorted by 2 groups: 1) Large-including Copepodite and adult Cyclopoid copepods and Cladocerans;2) Small nauplii and rotifers. It was found that in the northern part of the lake where turbidity is high, the density of small organisms is relatively lower, and in other parts of the lake where water is less turbid, large organisms are relatively less abundant. Speculative assumption about the impact of fish feeding behavior on that is presented.展开更多
Soybean （Glycine max （L.） Merr.） is a major crop that provides plant-origin protein and oil for humans and livestock. Al- though the soybean vegetative tissues and seeds provide a major source of high-quality prot...Soybean （Glycine max （L.） Merr.） is a major crop that provides plant-origin protein and oil for humans and livestock. Al- though the soybean vegetative tissues and seeds provide a major source of high-quality protein, they suffer from low con- centration of an essential sulfur-containing amino acid, methionine, which significantly limits their nutritional quality. The level of methionine is mainly controlled by the first unique enzyme of methionine synthesis, cystathione y-synthase （CGS）. Aiming to elevate methionine level in vegetative tissues and seeds, we constitutively over-expressed a feedback-insensitive Arabidopsis CGS （AtD-CGS） in soybean cultivars, Zigongdongdou （ZD） and Jilinxiaoli 1 （JX）. The levels of soluble methionine increased remarkably in leaves of transgenic soybeans compared to wild-type plants （6.6- and 7.3-fold in two transgenic ZD lines, and 3.7-fold in one transgenic JX line）. Furthermore, the total methionine contents were significantly increased in seeds of the transgenic ZD lines （1.5- to 4.8-fold increase） and the transgenic JX lines （1.3- to 2.3-fold increase） than in the wild type. The protein contents of the transgenic soybean seeds were significantly elevated compared to the wild type, suggesting that the scarcity of methionine in soybeans may limit protein accumulation in soybean seeds. The increased protein content did not alter the profile of major storage proteins in the seeds. Generally, this study provides a promising strategy to increase the levels of methionine and protein in soybean through the breeding programs.展开更多
Until 1957 most of the Hula Valley was occupied by swampy wetland covered by dense vegetation and old Lake Hula. Organic matter was accumulated in the bottom, decomposed under anoxic conditions creating Peat material....Until 1957 most of the Hula Valley was occupied by swampy wetland covered by dense vegetation and old Lake Hula. Organic matter was accumulated in the bottom, decomposed under anoxic conditions creating Peat material. The wetland and the old lake were drained and the land-use was converted into agricultural development. Nutrients migrations from the Hula Valley through the headwater discharges carrying nutrients, where nitrogen enhancement is critical, significantly affecting water quality in down-stream Lake Kinneret. The fate of the Hula originated nutrients is partly known whilst fate of the others which might be probably a threat on the Kinneret water quality is unknown. The hypsometrical and spatial distribution of the Hula Valley originated nutrients within three depths level was indicated: shallowest level of surface water, intermediate level of underground water table and the deepest level of Lignite waters. The Hypsometrical and spatial distribution and regional origin of the nutrient in the Hula Valley was defined. Organic Nitrogen, Sulfate and Nitrates are mostly Hula Valley originated nutrients whilst most of the Phosphorus externally contributed to Lake Kinneret originate outside the Hula Valley. An underground north-south Hydrological gradient and nutrient migration along was indicated. It is suggested that an underground plastic barrier do not totally prevent horizontal nutrient migration. Hypsometrical downward migrated nutrients probably accumulate within the “Lignite” depth level. Management policy of increasing Peat Soil moisture, is recommended.展开更多
Climate change conditions were indicated in the watershed of Lake Kinneret: air and consequently water temperature increase, decline in rainfall, and diminish river discharges accompanied by a reduction in Epilimnetic...Climate change conditions were indicated in the watershed of Lake Kinneret: air and consequently water temperature increase, decline in rainfall, and diminish river discharges accompanied by a reduction in Epilimnetic nitrogen and a slight increase in phosphorus. It is suggested that warming trend of the Kinneret Epilimnion enhanced decline of Zooplankton Biodiversity Index (ZBDI).展开更多
Fish (mostly Tilapia zillii), (TZ), mass mortality in Lake Kinneret initiated a study of the biology of TZ in Lake Kinneret. The study included several aspects: spawning and nesting behavior, feeding habits, the statu...Fish (mostly Tilapia zillii), (TZ), mass mortality in Lake Kinneret initiated a study of the biology of TZ in Lake Kinneret. The study included several aspects: spawning and nesting behavior, feeding habits, the status of TZ in the Kinneret fishery and temperature tolerance. The merit of TZ to commercial landing fishery is negligible, but this specimen is one of the most common fishes in the lake. Several environmental factors are considered as promoters of the epilimnetic temperature decline: Jordan water inputs in winter season as a heat source parameter. ENSO (EL-NIÑO/Southern Oscillation) acts as a cooling enhancement factor. Supportive data on the impact of ENSO were found in two terrestrial agricultural monitors in the northern and southern parts of the drainage basin. Gut content analysis of young fingerlings and an adult TZ specimen, indicated the significant contribution of benthic sources which are common in the shallows. The great adaptive capabilities of breeding TZ with various bottom habitats are demonstrated.展开更多
Long-Term (1946-2008) record of 35,580 measurements of daily mean air temperatures in three meteorological stations (Dan, Dafna, Kfar Blum) in the Hula Valley, within the drainage basin of Lake Kinneret (Israel) was s...Long-Term (1946-2008) record of 35,580 measurements of daily mean air temperatures in three meteorological stations (Dan, Dafna, Kfar Blum) in the Hula Valley, within the drainage basin of Lake Kinneret (Israel) was statistically evaluated. Temperature decline after the drainage of the old Lake Hula and adjacent wetlands (1958) and increase from the mid 1980’s, after the implementation of the Hula Project aimed at continuous land green cover were verified. It was suggested that those regional climate changes were due to the change of Albedo levels: lower when land was water covered and higher after regional drainage followed by a decline when vegetation cover became intensive and continuous. Decline of Albedo levels led to higher air temperature and vice versa.展开更多
Until the late 1950s the Hula Valley, located between altitude of 170 masl in the northern part and 61 masl in the southern part, was covered by the old shallow Lake Hula (1.5 m mean depth;1400 ha water surface);and 4...Until the late 1950s the Hula Valley, located between altitude of 170 masl in the northern part and 61 masl in the southern part, was covered by the old shallow Lake Hula (1.5 m mean depth;1400 ha water surface);and 4500 ha of swamps partly or permanently were water covered. During 1950-1957 the old Lake Hula and the wetlands were drained and converted into agricultural usage. As a result of inappropriate agricultural management, a reclamation project (Hula Project, HP, 1990-1997) was implemented. A vertical plastic sheet (4.5 m deep) was placed along 2.8 km across the valley aimed at reduction of pollutants migration. This plastic barrier divided the valley into northern, organic, and southern mineral soil blocks. The HP was aimed at agricultural renovation together with prevention of water quality deterioration in the downstream Lake Kinneret. The chemical composition of the underground waters was monitored on a monthly basis in 14 drills and ground water table observation wells over the valley during 14 months. The Ground Water Table (GWT) in the northern part of the valley was shallower than in the south and seasonal fluctuation amplitudes were smaller in the north. Higher levels of TP, TDP, P-Ortho and particulate Phosphorus, TN and ammonium were documented in the southern underground waters. The level of nutrient concentrations in the south was probably enhanced by three factors: 1) accumulation by underground water migration;2) eroded substances from the southern mineral soil;3) intensive Evapo-Transpiration in the south. Due to the lower level of organic content in the south and in spite of possible underground accumulation no significant difference between southern and northern blocks was indicated for Nitrate (NO3) concentrations. Not like nitrates, the reduced nitrogen form of ammonium concentration in the undergrounds was higher in the south. It was suggested that the nutrient concentrations (dissolved and suspended) in the Southern underground waters were higher than those in the North as a result of water migration, which took over the plastic barrier underneath and/or aside while moving from north to south was resulted by the hydraulic gradient. This gradient was partly due to the topographic slope and partly to the intensive Evapo-Transpiration in this part of the valley, which also contributed to the decline of GWT.展开更多
Russian sturgeon (<i></span><i><span style="font-family:Verdana;">Acipenser gueldenstaedtii</span></i><span style="font-family:Verdana;"></i></spa...Russian sturgeon (<i></span><i><span style="font-family:Verdana;">Acipenser gueldenstaedtii</span></i><span style="font-family:Verdana;"></i></span><span style="font-family:Verdana;">) is a primitive freshwater fish and a source of black caviar.</span></span><span style="font-family:""> </span><span style="font-family:""><span style="font-family:Verdana;">The genes involved in sexual determination and differentiation are still unknown and there are no molecular markers for sex identification in this species. Studying the variation of the sex-based differences in genomic sequences and in gene expression in the sturgeon may lead to markers of sex in early stages of development and advances in aquaculture, as well as provide novel insights about the evolution of reproduction, sex determination, and sexual differentiation mechanisms in vertebrates. Previous studies by our and other groups have identified differentially expressed genes in the gonads of adult female and male sturgeon. The current study aimed to test whether these ge</span><span style="font-family:Verdana;">nes were also differentially expressed in non-gonadal tissue, namely fins. We measured by qRT-PCR the mRNA levels of 29 known and novel sex-related genes in the gonads and fins of males (4 years old) and females (7 years old;sexual maturation is earlier in males than in females). Six genes (ATP6, IGFRM, LIA1A, S1A, NPL1A, GAPDH and SOX9) showed higher expression in female fin</span><span style="font-family:Verdana;">s. However, only ATP6 mRNA levels differed in fins of males and females of the same age (4 years old). These findings underscore the impracticality of sex identification based on gene expression in non-gonadal</span><span style="font-family:Verdana;"> tissue and the need for genetic sex markers in the Russian sturgeon.展开更多
Somatolactin (SL), a specific pituitary hormone belonging to the prolactin (PRL) super family, is involved in background adaptation, osmoregulation, reproduction and fatty acid metabolism. The goal of this study was t...Somatolactin (SL), a specific pituitary hormone belonging to the prolactin (PRL) super family, is involved in background adaptation, osmoregulation, reproduction and fatty acid metabolism. The goal of this study was to examine the gene transcription of SL changes in the ovary of blue gourami females (Trichogaster trichopterus) during oogenesis by quantitative real-time polymerase chain reaction (Real-Time PCR). Somatolactin in the pituitary was higher in females at low vitellogenesis compared to females with oocytes in maturation, and the difference was significant (p < 0.05). No significant differences were found in mRNA levels between low and high vitellogenesis, and high vitellogenesis and maturation. The findings of this and previous studies demonstrate that SL, growth hormone (GH) and PRL are involved in oogenesis in blue gourami;however, considerably more studies are required in order to separate the functions of these hormones.展开更多
The first decade of monitoring program in Lake Agmon (Hula Valley, Northern Israel) is summarized. The presented part of the program include: water discharges, physico-chemical (pH, EC, Temperature) and chemical param...The first decade of monitoring program in Lake Agmon (Hula Valley, Northern Israel) is summarized. The presented part of the program include: water discharges, physico-chemical (pH, EC, Temperature) and chemical parameters (TP, TN, TDN, TDP, NO<sub>3</sub>, NH<sub>4</sub>, SO<sub>4</sub>, TDS, TSS,). It was found that Nitrogen concentrations decline from north to south and the opposite for Phosphorus. Resulting of re-suspension by wind direction of western-southern-eastern and averaged maximal velocities of 9 - 10 m/s is suggested. De-nitrification and particles sedimentation emphasize Lake Agmon as a sink for Nitrogen. Sulfate-Carbonates association and the production of precipitated Gypsum (CaSO<sub>4</sub>) highlight the sink property of Lake Agmon. Nevertheless Sulfate removal by the Lake Agmon hydrological system is significant.展开更多
In this study, we examine male blue gourami (Trichogaster trichopterus) nest-building behavior that is affected by other males and interactions under experimental conditions. Males found in the area control the territ...In this study, we examine male blue gourami (Trichogaster trichopterus) nest-building behavior that is affected by other males and interactions under experimental conditions. Males found in the area control the territory and sexual behavior, and are influenced by the behavior of other males. The results of this study show that the sexual behavior (nest-building) of male blue gourami is affected both by the behavior and pheromones of other males. We suggest that males must defend their territories in order to prevent these two factors from interfering in nest-building.展开更多
The present study examines the inherent flexibility in the feeding responses of S. infraimmaculata larvae to various breeding places. In addition, the study examines the hypothesis that in dry conditions, the water br...The present study examines the inherent flexibility in the feeding responses of S. infraimmaculata larvae to various breeding places. In addition, the study examines the hypothesis that in dry conditions, the water breeding places are the limiting factor for the survival and completion of the life cycle of populations of S. infraimmaculata. In the breeding sites, the food for larvae is not available, and cannibalism is the specific adaptation for larvae to grow and complete metamorphosis. S. infraimmaculata larvae feed on various invertebrates in different habitats, and cannibalism involves the adaptation to breeding places where food is not available. The growth only by cannibalism as the only way to adapt to these habitats because no food is found in breeding places at the southern border of its distribution is described here for the first time, not only in salamanders but in other animals as well.展开更多
Two models have been developed for the evaluation and prediction of salinity changes (chloride concentration;ppm;[Cl]) in Lake Kinneret. They are: 1) RM, which is based on the Salt balance and hydrological budget of t...Two models have been developed for the evaluation and prediction of salinity changes (chloride concentration;ppm;[Cl]) in Lake Kinneret. They are: 1) RM, which is based on the Salt balance and hydrological budget of the lake;2) GM which is based on the geo-hydrological regional properties of Kinneret’s geological structure. The concept of both is partly different: RM is correlating reduction of runoffs to salinity elevation and GM relates salt flux to the aquifer yield and the impact of lake water level is neglected. Long term statistical analyses justify support to GM, excluding conditions of heavy floods. This paper is a combination of a supplemental extended temporal study and a models review. Practical conclusions on Kinneret hydrological management within a frame of constrains aimed at prevention of salinity and eutrophication increase are presented.展开更多
Reports on the Kinneret zooplankton in general and particularly on copepods are dealing recently with numerical densities of feeding habit classes. Nevertheless, for the evaluation of the integrated role of copepod as...Reports on the Kinneret zooplankton in general and particularly on copepods are dealing recently with numerical densities of feeding habit classes. Nevertheless, for the evaluation of the integrated role of copepod assemblages in the entire lake ecosystem, their life cycle stages fluctuations are essential. It was not done in previous reports. Therefore, long term analysis of the cyclopoid copepods life stages dynamics in Lake Kinneret was carried out. Due to information availability, two complementary methods of density concentrations were evaluated for two consecutive data sets: 1) 1969-1985 numerical (No/L) documentation of life cycle stages of nauplii (I - III and III - V), copepodites (I, II, III, IV, V) and adults;2) 1969-2002 monthly averages of wet biomass density (g/m<sup>2</sup>) of zooplankton major groups combined with metabolic rates. nauplii densities represent June-August peaks and older stages spring decline and high levels during summer-early winter. Polynomial Regressions between Numerical annual averages of predator and Herbivore stages as well as young vs. older life stages indicate significant relations. During winter-spring season, temperatures are optimal for cyclopoid growth as a result of the high efficiency of metabolic activity and the low pressure of fish predation. The high rate of metabolism and intensive fish predation in summer enhance low densities of adult cyclopoids.展开更多
The Lake Kinneret (Israel) ecosystem has undergone limnological changes. The trend of changes started in the mid 1990’s. The deviation from the previously known as stable long-term structure of the ecosystem included...The Lake Kinneret (Israel) ecosystem has undergone limnological changes. The trend of changes started in the mid 1990’s. The deviation from the previously known as stable long-term structure of the ecosystem included, among others, phytoplankton community structure, nutrient dynamics, zooplankton densities, water temperature increase, water level decline, and fishery management. This study is aimed at identifying ecosystem stability by focusing on three major compartments of the ecosystem: Total Dissolved Solids (TSS), Plankton (Phyto and Zoo), and Detritus (TSS minus plankton). It is suggested that although significant temporary changes of biotic and abiotic parameters were recorded and despite the existence of the normal seasonal fluctuations, the major compartments’ structural relations were not modified. The objective of the study is to analyze the process initiation of the modification trend. Previous studies and reports were focused on descriptive evaluation of the modified parameters. The expected outcome is a comprehensive evaluation of the modification process. Consequently, resulted prediction might be a tool for managers to improve management design.展开更多
The purpose of the present study was to examine the genetic variation in Salamandra infraimmaculata from different breeding site habitats using the Amplified Fragment Length Polymorphism (AFLP) method. The results of ...The purpose of the present study was to examine the genetic variation in Salamandra infraimmaculata from different breeding site habitats using the Amplified Fragment Length Polymorphism (AFLP) method. The results of the dendogram from a hierarchical cluster analysis show that the grouping of S. infraimmaculata as cluster 5 differs from all the other clusters, including the St1 (Tel-Dan stream) population, which was the most predictable. Five Haplogroups (Hg) were characterized. The mean number of alleles per locus in each population (Ne) ranged from 10.566 (Sp1) to 2.720 (Po6). An average estimated heterozygosity (He) by population ranged from 0.100 (Po6) to 0.186 (St1). Population St1, a permanent breeding site where water was available all year round, exhibited the highest level of polymorphism, while population Po6, from the ephemeral breeding site, exhibited the lowest level of polymorphism. Gene flow between clusters showed that clusters 3 and 4 are sources of migrants and also receive gene flow, while clusters 1 and 2 may be a source of migrants but may not receive much gene flow. A phylogenetic analysis, based on clustering using Nei’s genetic distance, demonstrated that the Tel-Dan population is located on a separate branch within its sub-population. The conclusion of the present study shows that the genetic divergence among isolated populations is not correlated to distance but is affected by the variation of habitats.展开更多
The development of new agricultural industries is an important challenge for a region’s economic establishment. The connection between a research department at MIGAL Institute and biotechnology in aquaculture is an e...The development of new agricultural industries is an important challenge for a region’s economic establishment. The connection between a research department at MIGAL Institute and biotechnology in aquaculture is an example (case study) of the interrelationships that also exist in other departments. This article describes the research conducted by the institute supporting the development of the aquaculture industry in Israel through the introduction of a relatively new fish species—the Russian sturgeon (Acipenser gueldenstaedtii), with the relevant information to be used for acclimatization to aquaculture conditions in northern Israel. Many aspects related to bringing a new species to Israel and working with this new industry to study its adaptation to existing conditions in the north of the country influenced the economic success of this new type of precision agriculture. This article describes the research on various aspects affecting the successful acclimatization of Russian sturgeon in northern Israel: introduction, food development, reproduction, hormone systems that control reproduction and growth, gonadal development, and molecular markers for sex determination, differentiation, and economic assessment of caviar production.展开更多
BACKGROUND In recent years,associations between specific virulence markers of Helicobacter pylori(H.pylori)and gastrointestinal disorders have been suggested.AIM To investigate the presence of virulence factors includ...BACKGROUND In recent years,associations between specific virulence markers of Helicobacter pylori(H.pylori)and gastrointestinal disorders have been suggested.AIM To investigate the presence of virulence factors including vacuolating cytotoxin A genotypes(s1m1,s1m2,s2m1,and s2m2),cytotoxin-associated gene A(CagA),and urease activity in H.pylori strains isolated from Arab and Jewish populations in northern Israel and to assess associations between these factors and patients’demographics and clinical outcomes.METHODS Patients(n=108)who underwent gastroscopy at the Baruch Padeh Medical Center,Poriya due to symptomatic gastroduodenal pathologies as part of H.pylori diagnosis were enrolled in the study.Gastric biopsy specimens were collected from the antrum of the stomach.Clinical condition was assessed by clinical pathology tests.Bacteria were isolated on modified BD Helicobacter Agar(BD Diagnostics,Sparks,MD,United States).Bacterial DNA was extracted,and PCR was performed to detect CagA and vacuolating cytotoxin A genes.Urease activity was assessed using a rapid urease test.RESULTS A significant correlation was found between disease severity and patient ethnicity(P=0.002).A significant correlation was found between CagA presence and the s1m1 genotype(P=0.02),which is considered the most virulent genotype.Further,a higher level of urease activity was associated with isolates originating from the Jewish population.Moreover,higher urease activity levels were measured among CagA-/s1m1 and CagA-/s2m2 isolates.CONCLUSION Our study highlights the importance of incorporating molecular methods for detection of virulence markers of H.pylori in order to tailor optimal treatments for each patient.Further investigation should be performed regarding associations between H.pylori virulence factors and ethnicity.展开更多
<span style="font-family:Verdana;">This study examined the relationship between tree frog</span> (<i><i><span style="font-family:Verdana;">Hyla</span></i>&...<span style="font-family:Verdana;">This study examined the relationship between tree frog</span> (<i><i><span style="font-family:Verdana;">Hyla</span></i><span> <i><span style="font-family:Verdana;">savignyi</span></i><span style="font-family:Verdana;"></span></span></i><span style="font-family:Verdana;">)</span><span style="font-family:Verdana;"> coloring and its different seasonal habitats at the southern border of its distribution. The results show that tree frog color is affected by the dominant colors in its habitat, which vary seasonally, especially between winter and summer. Tree frog colors were various shades of green, white, brown, and black. No genetic marker was found to characterize the color. The ability of a small frog to infer its own time with the help of color changes occurring in the habitat on the southern border of its distribution, which are relatively broad, gives this species an advantage.</span>展开更多
Several ecological key factors were indicated in the Lake Kinneret ecosystem during 1969-2000: Elevation of the biomass of non-pyrrhophyte-phytoplankton, chlorophyta, cyanobacteria, and diatoms;decline of Peridinium m...Several ecological key factors were indicated in the Lake Kinneret ecosystem during 1969-2000: Elevation of the biomass of non-pyrrhophyte-phytoplankton, chlorophyta, cyanobacteria, and diatoms;decline of Peridinium maximal from 215 - 240 to 175 - 200 ranges (g/m2);decline of zooplankton (herbivore and predator) relative to phytoplankton biomass (g/m2);lower loads of Nitrogen and slightly also phosphorus in the river Jordan discharge;decline of precipitations and lake water level;significant decline of epilimnetic nitrogen and minor changes of phosphorus concentrations initiated decline of N/P mass ratio to the establishment of a significant change of the ecosystem to be modified from P to N limitation. What could be other than essential outcome of future prediction that results of 20 years (1969-2000) of routine and comprehensive monitor carried out in Lake Kinneret initiated? The Lake Kinneret ecosystem dynamics after 2000 justified retroactive post-factum earlier conclusion of appropriate predictability.展开更多
文摘Spatial survey of zooplankton community composition within the upper 10 m layer in Lake Kinneret was carried out. Samples were collected by plankton net (63 μ mesh size) and counted organisms were sorted by 2 groups: 1) Large-including Copepodite and adult Cyclopoid copepods and Cladocerans;2) Small nauplii and rotifers. It was found that in the northern part of the lake where turbidity is high, the density of small organisms is relatively lower, and in other parts of the lake where water is less turbid, large organisms are relatively less abundant. Speculative assumption about the impact of fish feeding behavior on that is presented.
基金supported by the Major Science and Technology Projects of China (2014ZX08004-003,2014ZX08010-004)the Natural Science Foundation of China (31471571)+2 种基金the earmarked fund for China Agriculture Research System (CARS-04)the Agricultural Science and Technology Innovation Project of CAASIsrael Science Foundation (ISF grant 231-09)
文摘Soybean （Glycine max （L.） Merr.） is a major crop that provides plant-origin protein and oil for humans and livestock. Al- though the soybean vegetative tissues and seeds provide a major source of high-quality protein, they suffer from low con- centration of an essential sulfur-containing amino acid, methionine, which significantly limits their nutritional quality. The level of methionine is mainly controlled by the first unique enzyme of methionine synthesis, cystathione y-synthase （CGS）. Aiming to elevate methionine level in vegetative tissues and seeds, we constitutively over-expressed a feedback-insensitive Arabidopsis CGS （AtD-CGS） in soybean cultivars, Zigongdongdou （ZD） and Jilinxiaoli 1 （JX）. The levels of soluble methionine increased remarkably in leaves of transgenic soybeans compared to wild-type plants （6.6- and 7.3-fold in two transgenic ZD lines, and 3.7-fold in one transgenic JX line）. Furthermore, the total methionine contents were significantly increased in seeds of the transgenic ZD lines （1.5- to 4.8-fold increase） and the transgenic JX lines （1.3- to 2.3-fold increase） than in the wild type. The protein contents of the transgenic soybean seeds were significantly elevated compared to the wild type, suggesting that the scarcity of methionine in soybeans may limit protein accumulation in soybean seeds. The increased protein content did not alter the profile of major storage proteins in the seeds. Generally, this study provides a promising strategy to increase the levels of methionine and protein in soybean through the breeding programs.
文摘Until 1957 most of the Hula Valley was occupied by swampy wetland covered by dense vegetation and old Lake Hula. Organic matter was accumulated in the bottom, decomposed under anoxic conditions creating Peat material. The wetland and the old lake were drained and the land-use was converted into agricultural development. Nutrients migrations from the Hula Valley through the headwater discharges carrying nutrients, where nitrogen enhancement is critical, significantly affecting water quality in down-stream Lake Kinneret. The fate of the Hula originated nutrients is partly known whilst fate of the others which might be probably a threat on the Kinneret water quality is unknown. The hypsometrical and spatial distribution of the Hula Valley originated nutrients within three depths level was indicated: shallowest level of surface water, intermediate level of underground water table and the deepest level of Lignite waters. The Hypsometrical and spatial distribution and regional origin of the nutrient in the Hula Valley was defined. Organic Nitrogen, Sulfate and Nitrates are mostly Hula Valley originated nutrients whilst most of the Phosphorus externally contributed to Lake Kinneret originate outside the Hula Valley. An underground north-south Hydrological gradient and nutrient migration along was indicated. It is suggested that an underground plastic barrier do not totally prevent horizontal nutrient migration. Hypsometrical downward migrated nutrients probably accumulate within the “Lignite” depth level. Management policy of increasing Peat Soil moisture, is recommended.
文摘Climate change conditions were indicated in the watershed of Lake Kinneret: air and consequently water temperature increase, decline in rainfall, and diminish river discharges accompanied by a reduction in Epilimnetic nitrogen and a slight increase in phosphorus. It is suggested that warming trend of the Kinneret Epilimnion enhanced decline of Zooplankton Biodiversity Index (ZBDI).
文摘Fish (mostly Tilapia zillii), (TZ), mass mortality in Lake Kinneret initiated a study of the biology of TZ in Lake Kinneret. The study included several aspects: spawning and nesting behavior, feeding habits, the status of TZ in the Kinneret fishery and temperature tolerance. The merit of TZ to commercial landing fishery is negligible, but this specimen is one of the most common fishes in the lake. Several environmental factors are considered as promoters of the epilimnetic temperature decline: Jordan water inputs in winter season as a heat source parameter. ENSO (EL-NIÑO/Southern Oscillation) acts as a cooling enhancement factor. Supportive data on the impact of ENSO were found in two terrestrial agricultural monitors in the northern and southern parts of the drainage basin. Gut content analysis of young fingerlings and an adult TZ specimen, indicated the significant contribution of benthic sources which are common in the shallows. The great adaptive capabilities of breeding TZ with various bottom habitats are demonstrated.
文摘Long-Term (1946-2008) record of 35,580 measurements of daily mean air temperatures in three meteorological stations (Dan, Dafna, Kfar Blum) in the Hula Valley, within the drainage basin of Lake Kinneret (Israel) was statistically evaluated. Temperature decline after the drainage of the old Lake Hula and adjacent wetlands (1958) and increase from the mid 1980’s, after the implementation of the Hula Project aimed at continuous land green cover were verified. It was suggested that those regional climate changes were due to the change of Albedo levels: lower when land was water covered and higher after regional drainage followed by a decline when vegetation cover became intensive and continuous. Decline of Albedo levels led to higher air temperature and vice versa.
文摘Until the late 1950s the Hula Valley, located between altitude of 170 masl in the northern part and 61 masl in the southern part, was covered by the old shallow Lake Hula (1.5 m mean depth;1400 ha water surface);and 4500 ha of swamps partly or permanently were water covered. During 1950-1957 the old Lake Hula and the wetlands were drained and converted into agricultural usage. As a result of inappropriate agricultural management, a reclamation project (Hula Project, HP, 1990-1997) was implemented. A vertical plastic sheet (4.5 m deep) was placed along 2.8 km across the valley aimed at reduction of pollutants migration. This plastic barrier divided the valley into northern, organic, and southern mineral soil blocks. The HP was aimed at agricultural renovation together with prevention of water quality deterioration in the downstream Lake Kinneret. The chemical composition of the underground waters was monitored on a monthly basis in 14 drills and ground water table observation wells over the valley during 14 months. The Ground Water Table (GWT) in the northern part of the valley was shallower than in the south and seasonal fluctuation amplitudes were smaller in the north. Higher levels of TP, TDP, P-Ortho and particulate Phosphorus, TN and ammonium were documented in the southern underground waters. The level of nutrient concentrations in the south was probably enhanced by three factors: 1) accumulation by underground water migration;2) eroded substances from the southern mineral soil;3) intensive Evapo-Transpiration in the south. Due to the lower level of organic content in the south and in spite of possible underground accumulation no significant difference between southern and northern blocks was indicated for Nitrate (NO3) concentrations. Not like nitrates, the reduced nitrogen form of ammonium concentration in the undergrounds was higher in the south. It was suggested that the nutrient concentrations (dissolved and suspended) in the Southern underground waters were higher than those in the North as a result of water migration, which took over the plastic barrier underneath and/or aside while moving from north to south was resulted by the hydraulic gradient. This gradient was partly due to the topographic slope and partly to the intensive Evapo-Transpiration in this part of the valley, which also contributed to the decline of GWT.
文摘Russian sturgeon (<i></span><i><span style="font-family:Verdana;">Acipenser gueldenstaedtii</span></i><span style="font-family:Verdana;"></i></span><span style="font-family:Verdana;">) is a primitive freshwater fish and a source of black caviar.</span></span><span style="font-family:""> </span><span style="font-family:""><span style="font-family:Verdana;">The genes involved in sexual determination and differentiation are still unknown and there are no molecular markers for sex identification in this species. Studying the variation of the sex-based differences in genomic sequences and in gene expression in the sturgeon may lead to markers of sex in early stages of development and advances in aquaculture, as well as provide novel insights about the evolution of reproduction, sex determination, and sexual differentiation mechanisms in vertebrates. Previous studies by our and other groups have identified differentially expressed genes in the gonads of adult female and male sturgeon. The current study aimed to test whether these ge</span><span style="font-family:Verdana;">nes were also differentially expressed in non-gonadal tissue, namely fins. We measured by qRT-PCR the mRNA levels of 29 known and novel sex-related genes in the gonads and fins of males (4 years old) and females (7 years old;sexual maturation is earlier in males than in females). Six genes (ATP6, IGFRM, LIA1A, S1A, NPL1A, GAPDH and SOX9) showed higher expression in female fin</span><span style="font-family:Verdana;">s. However, only ATP6 mRNA levels differed in fins of males and females of the same age (4 years old). These findings underscore the impracticality of sex identification based on gene expression in non-gonadal</span><span style="font-family:Verdana;"> tissue and the need for genetic sex markers in the Russian sturgeon.
文摘Somatolactin (SL), a specific pituitary hormone belonging to the prolactin (PRL) super family, is involved in background adaptation, osmoregulation, reproduction and fatty acid metabolism. The goal of this study was to examine the gene transcription of SL changes in the ovary of blue gourami females (Trichogaster trichopterus) during oogenesis by quantitative real-time polymerase chain reaction (Real-Time PCR). Somatolactin in the pituitary was higher in females at low vitellogenesis compared to females with oocytes in maturation, and the difference was significant (p < 0.05). No significant differences were found in mRNA levels between low and high vitellogenesis, and high vitellogenesis and maturation. The findings of this and previous studies demonstrate that SL, growth hormone (GH) and PRL are involved in oogenesis in blue gourami;however, considerably more studies are required in order to separate the functions of these hormones.
文摘The first decade of monitoring program in Lake Agmon (Hula Valley, Northern Israel) is summarized. The presented part of the program include: water discharges, physico-chemical (pH, EC, Temperature) and chemical parameters (TP, TN, TDN, TDP, NO<sub>3</sub>, NH<sub>4</sub>, SO<sub>4</sub>, TDS, TSS,). It was found that Nitrogen concentrations decline from north to south and the opposite for Phosphorus. Resulting of re-suspension by wind direction of western-southern-eastern and averaged maximal velocities of 9 - 10 m/s is suggested. De-nitrification and particles sedimentation emphasize Lake Agmon as a sink for Nitrogen. Sulfate-Carbonates association and the production of precipitated Gypsum (CaSO<sub>4</sub>) highlight the sink property of Lake Agmon. Nevertheless Sulfate removal by the Lake Agmon hydrological system is significant.
文摘In this study, we examine male blue gourami (Trichogaster trichopterus) nest-building behavior that is affected by other males and interactions under experimental conditions. Males found in the area control the territory and sexual behavior, and are influenced by the behavior of other males. The results of this study show that the sexual behavior (nest-building) of male blue gourami is affected both by the behavior and pheromones of other males. We suggest that males must defend their territories in order to prevent these two factors from interfering in nest-building.
文摘The present study examines the inherent flexibility in the feeding responses of S. infraimmaculata larvae to various breeding places. In addition, the study examines the hypothesis that in dry conditions, the water breeding places are the limiting factor for the survival and completion of the life cycle of populations of S. infraimmaculata. In the breeding sites, the food for larvae is not available, and cannibalism is the specific adaptation for larvae to grow and complete metamorphosis. S. infraimmaculata larvae feed on various invertebrates in different habitats, and cannibalism involves the adaptation to breeding places where food is not available. The growth only by cannibalism as the only way to adapt to these habitats because no food is found in breeding places at the southern border of its distribution is described here for the first time, not only in salamanders but in other animals as well.
文摘Two models have been developed for the evaluation and prediction of salinity changes (chloride concentration;ppm;[Cl]) in Lake Kinneret. They are: 1) RM, which is based on the Salt balance and hydrological budget of the lake;2) GM which is based on the geo-hydrological regional properties of Kinneret’s geological structure. The concept of both is partly different: RM is correlating reduction of runoffs to salinity elevation and GM relates salt flux to the aquifer yield and the impact of lake water level is neglected. Long term statistical analyses justify support to GM, excluding conditions of heavy floods. This paper is a combination of a supplemental extended temporal study and a models review. Practical conclusions on Kinneret hydrological management within a frame of constrains aimed at prevention of salinity and eutrophication increase are presented.
文摘Reports on the Kinneret zooplankton in general and particularly on copepods are dealing recently with numerical densities of feeding habit classes. Nevertheless, for the evaluation of the integrated role of copepod assemblages in the entire lake ecosystem, their life cycle stages fluctuations are essential. It was not done in previous reports. Therefore, long term analysis of the cyclopoid copepods life stages dynamics in Lake Kinneret was carried out. Due to information availability, two complementary methods of density concentrations were evaluated for two consecutive data sets: 1) 1969-1985 numerical (No/L) documentation of life cycle stages of nauplii (I - III and III - V), copepodites (I, II, III, IV, V) and adults;2) 1969-2002 monthly averages of wet biomass density (g/m<sup>2</sup>) of zooplankton major groups combined with metabolic rates. nauplii densities represent June-August peaks and older stages spring decline and high levels during summer-early winter. Polynomial Regressions between Numerical annual averages of predator and Herbivore stages as well as young vs. older life stages indicate significant relations. During winter-spring season, temperatures are optimal for cyclopoid growth as a result of the high efficiency of metabolic activity and the low pressure of fish predation. The high rate of metabolism and intensive fish predation in summer enhance low densities of adult cyclopoids.
文摘The Lake Kinneret (Israel) ecosystem has undergone limnological changes. The trend of changes started in the mid 1990’s. The deviation from the previously known as stable long-term structure of the ecosystem included, among others, phytoplankton community structure, nutrient dynamics, zooplankton densities, water temperature increase, water level decline, and fishery management. This study is aimed at identifying ecosystem stability by focusing on three major compartments of the ecosystem: Total Dissolved Solids (TSS), Plankton (Phyto and Zoo), and Detritus (TSS minus plankton). It is suggested that although significant temporary changes of biotic and abiotic parameters were recorded and despite the existence of the normal seasonal fluctuations, the major compartments’ structural relations were not modified. The objective of the study is to analyze the process initiation of the modification trend. Previous studies and reports were focused on descriptive evaluation of the modified parameters. The expected outcome is a comprehensive evaluation of the modification process. Consequently, resulted prediction might be a tool for managers to improve management design.
文摘The purpose of the present study was to examine the genetic variation in Salamandra infraimmaculata from different breeding site habitats using the Amplified Fragment Length Polymorphism (AFLP) method. The results of the dendogram from a hierarchical cluster analysis show that the grouping of S. infraimmaculata as cluster 5 differs from all the other clusters, including the St1 (Tel-Dan stream) population, which was the most predictable. Five Haplogroups (Hg) were characterized. The mean number of alleles per locus in each population (Ne) ranged from 10.566 (Sp1) to 2.720 (Po6). An average estimated heterozygosity (He) by population ranged from 0.100 (Po6) to 0.186 (St1). Population St1, a permanent breeding site where water was available all year round, exhibited the highest level of polymorphism, while population Po6, from the ephemeral breeding site, exhibited the lowest level of polymorphism. Gene flow between clusters showed that clusters 3 and 4 are sources of migrants and also receive gene flow, while clusters 1 and 2 may be a source of migrants but may not receive much gene flow. A phylogenetic analysis, based on clustering using Nei’s genetic distance, demonstrated that the Tel-Dan population is located on a separate branch within its sub-population. The conclusion of the present study shows that the genetic divergence among isolated populations is not correlated to distance but is affected by the variation of habitats.
文摘The development of new agricultural industries is an important challenge for a region’s economic establishment. The connection between a research department at MIGAL Institute and biotechnology in aquaculture is an example (case study) of the interrelationships that also exist in other departments. This article describes the research conducted by the institute supporting the development of the aquaculture industry in Israel through the introduction of a relatively new fish species—the Russian sturgeon (Acipenser gueldenstaedtii), with the relevant information to be used for acclimatization to aquaculture conditions in northern Israel. Many aspects related to bringing a new species to Israel and working with this new industry to study its adaptation to existing conditions in the north of the country influenced the economic success of this new type of precision agriculture. This article describes the research on various aspects affecting the successful acclimatization of Russian sturgeon in northern Israel: introduction, food development, reproduction, hormone systems that control reproduction and growth, gonadal development, and molecular markers for sex determination, differentiation, and economic assessment of caviar production.
基金The study was reviewed and approved by the Helsinki Committee of the Baruch Padeh Medical Center,Poriya(Approval No.POR 0007-20).
文摘BACKGROUND In recent years,associations between specific virulence markers of Helicobacter pylori(H.pylori)and gastrointestinal disorders have been suggested.AIM To investigate the presence of virulence factors including vacuolating cytotoxin A genotypes(s1m1,s1m2,s2m1,and s2m2),cytotoxin-associated gene A(CagA),and urease activity in H.pylori strains isolated from Arab and Jewish populations in northern Israel and to assess associations between these factors and patients’demographics and clinical outcomes.METHODS Patients(n=108)who underwent gastroscopy at the Baruch Padeh Medical Center,Poriya due to symptomatic gastroduodenal pathologies as part of H.pylori diagnosis were enrolled in the study.Gastric biopsy specimens were collected from the antrum of the stomach.Clinical condition was assessed by clinical pathology tests.Bacteria were isolated on modified BD Helicobacter Agar(BD Diagnostics,Sparks,MD,United States).Bacterial DNA was extracted,and PCR was performed to detect CagA and vacuolating cytotoxin A genes.Urease activity was assessed using a rapid urease test.RESULTS A significant correlation was found between disease severity and patient ethnicity(P=0.002).A significant correlation was found between CagA presence and the s1m1 genotype(P=0.02),which is considered the most virulent genotype.Further,a higher level of urease activity was associated with isolates originating from the Jewish population.Moreover,higher urease activity levels were measured among CagA-/s1m1 and CagA-/s2m2 isolates.CONCLUSION Our study highlights the importance of incorporating molecular methods for detection of virulence markers of H.pylori in order to tailor optimal treatments for each patient.Further investigation should be performed regarding associations between H.pylori virulence factors and ethnicity.
文摘<span style="font-family:Verdana;">This study examined the relationship between tree frog</span> (<i><i><span style="font-family:Verdana;">Hyla</span></i><span> <i><span style="font-family:Verdana;">savignyi</span></i><span style="font-family:Verdana;"></span></span></i><span style="font-family:Verdana;">)</span><span style="font-family:Verdana;"> coloring and its different seasonal habitats at the southern border of its distribution. The results show that tree frog color is affected by the dominant colors in its habitat, which vary seasonally, especially between winter and summer. Tree frog colors were various shades of green, white, brown, and black. No genetic marker was found to characterize the color. The ability of a small frog to infer its own time with the help of color changes occurring in the habitat on the southern border of its distribution, which are relatively broad, gives this species an advantage.</span>
文摘Several ecological key factors were indicated in the Lake Kinneret ecosystem during 1969-2000: Elevation of the biomass of non-pyrrhophyte-phytoplankton, chlorophyta, cyanobacteria, and diatoms;decline of Peridinium maximal from 215 - 240 to 175 - 200 ranges (g/m2);decline of zooplankton (herbivore and predator) relative to phytoplankton biomass (g/m2);lower loads of Nitrogen and slightly also phosphorus in the river Jordan discharge;decline of precipitations and lake water level;significant decline of epilimnetic nitrogen and minor changes of phosphorus concentrations initiated decline of N/P mass ratio to the establishment of a significant change of the ecosystem to be modified from P to N limitation. What could be other than essential outcome of future prediction that results of 20 years (1969-2000) of routine and comprehensive monitor carried out in Lake Kinneret initiated? The Lake Kinneret ecosystem dynamics after 2000 justified retroactive post-factum earlier conclusion of appropriate predictability.
