Xylonic acid is a versatile and valuable chemical with a range of industrial and potential health applications.The high demand for renewable energy and sustainable products has increased interest in xylonic acid as a ...Xylonic acid is a versatile and valuable chemical with a range of industrial and potential health applications.The high demand for renewable energy and sustainable products has increased interest in xylonic acid as a starting material for the production of variety of high value chemicals and biofuels.The production of xylonic acid from biomass have an added advantage that it provides an opportunity to utilize bio-waste materials that would otherwise be discarded,reducing environmental hazards and reducing waste disposal costs.The aim of this research was to establish a bioprocess for producing xylonic acid from acid pre-treated sawdust liquor via fermentation,utilizing metabolically engineered strain of Corynebacterium glutamicum ATCC 31831.A maximum titre of 48.5±0.2 g/L xylonic acid from 60 g/L xylose derived from biomass was obtained with a yield of 0.89 g/g xylose which corresponds to a conversion of 62%.30.8±0.4 g/L of xylonic acid crystals of 95.5%purity were successfully recovered from 1L sawdust APL(Acid Pre-treated Liquor)via solvent precipitation and crystallization.Purified xylonic acid crystals were checked for its antibacterial effect on Salmonella enterica MTCC 3224,Escherichia coli MTCC 443 and Staphylococcus aureus MTCC 96.展开更多
Microalgae are considered a rich source of high-value metabolites with an array of nutraceutical and pharmaceutical applications.Diferent strategies have been developed for cultivating microalgae at large-scale photob...Microalgae are considered a rich source of high-value metabolites with an array of nutraceutical and pharmaceutical applications.Diferent strategies have been developed for cultivating microalgae at large-scale photobioreactors but high cost and low productivity are the major hurdles.Optimizing the composition of media for the cultivation of microalgae to induce biomass production and high-value metabolite accumulation has been considered as an important factor for sustainable product development.In this study,the efect of plant growth regulators together with basal microalgal cultivation medium on biomass,total lipid,and EPA production was studied using the Plackett–Burman model and Response surface methodology.The traditional one-factor-at-a-time optimization approach is laborious,time-consuming,and requires more experiments which makes the process and analysis more difcult.The Designed PB model was found to be signifcant for biomass(396 mg/L),lipid(254 mg/L),and EPA(5.6%)production with a P value<0.05.The major objective of this study is to formulate a medium for EPA production without compromising the growth properties.Further,we had formulated a new media using RSM to achieve the goal and the signifcant variables selected were NaNO_(3),NaH_(2)PO_(4),and IAA and was found to be signifcant with 16.72%EPA production with a biomass production of 893 mg/L with a P value<0.05.The formulated medium can be used in large-scale cultivation systems which can enhance biomass production as well as the omega 3 fatty acid production in marine microalgae Nannochloropsis oceanica.展开更多
Hydrothermal liquefaction(HTL)and pyrolysis(Py)of Loktak lake biomass mixture of water hyacinth(WH)and para grass(PG)were carried out at 260-300℃ and 300-500℃ to compare the products yield and chemical characteristi...Hydrothermal liquefaction(HTL)and pyrolysis(Py)of Loktak lake biomass mixture of water hyacinth(WH)and para grass(PG)were carried out at 260-300℃ and 300-500℃ to compare the products yield and chemical characteristics of the products.In case of HTL,the maximum bio-oil yield was obtained 13.34 wt.%at 280℃ while for Py,the maximum bio-oil yield was observed 38.8 wt.%at 350℃.The obtained bio-oils and bio-chars were analyzed using GC-MS,FT-IR,NMR,TGA,TOC,and SEM.GC-MS analysis of the bio-oils were showed that the HTL bio-oil majorly contains of nitrogen containing compounds whereas the Py bio-oil contains majority of phenolic compounds.Other compounds like ketones,alcohols,acids were also observed in bio-oil.Higher intensity broad band at 3300-3500 cm^(−1) was observed in the Py bio-oil compared to HTL bio-oil.TGA and proximate analysis of bio-char revealed the higher devolatilization occured during the HTL compared to Py process.The surface morphology of the HTL bio-char was found to be rough and fragmented as compared to the pyrolysis bio-char,clearly showing the biomass macromolecules breakdown differently in HTL and Py process.展开更多
Byssochlamys fulva AM130,a novel strain of filamentous fungus,could produce ethanol from glucose,xylose,and alkali pretreated rice straw(PRS),while the efficiencies were very low with PRS.Ethanol production of 11.84 g...Byssochlamys fulva AM130,a novel strain of filamentous fungus,could produce ethanol from glucose,xylose,and alkali pretreated rice straw(PRS),while the efficiencies were very low with PRS.Ethanol production of 11.84 g/L was attained by the fungus when grown in glucose,indicating that the limitations while growing on PRS were related to low hydrolytic efficiency.Enzyme profiling of the fungus showed 365 IU/ml of beta-glucosidase and 89 IU/ml of xylanase activity,while endoglucanase and filter paper activity were negligible,which accounts for the low hydrolytic efficiency.The fungus could survive for extended periods under oxygen-limited conditions and produce ethanol.The fungal mycelia could also be used for repeated cycles of anaerobic fermentation,wherein the ethanol yield improved with each consecutive cycle.展开更多
Problems with food security result from increased population,global warming,and decrease in cultivable land.With the advancements in synthetic biology,microbial synthesis of food is considered to be an efficient alter...Problems with food security result from increased population,global warming,and decrease in cultivable land.With the advancements in synthetic biology,microbial synthesis of food is considered to be an efficient alternate approach that could permit quick food biosynthesis in an eco-friendly method.Furthermore,synthetic biology can be assumed to the synthesis of healthy or specially designed food components like proteins,lipids,amino acids and vitamins and widen the consumption of feedstocks,thus offering possible resolutions to high-quality food synthesis.This review describes the impact of synthetic biology for the microbial synthesis of various food ingredients production.展开更多
基金The funding has been received from CSIR New Delhi.
文摘Xylonic acid is a versatile and valuable chemical with a range of industrial and potential health applications.The high demand for renewable energy and sustainable products has increased interest in xylonic acid as a starting material for the production of variety of high value chemicals and biofuels.The production of xylonic acid from biomass have an added advantage that it provides an opportunity to utilize bio-waste materials that would otherwise be discarded,reducing environmental hazards and reducing waste disposal costs.The aim of this research was to establish a bioprocess for producing xylonic acid from acid pre-treated sawdust liquor via fermentation,utilizing metabolically engineered strain of Corynebacterium glutamicum ATCC 31831.A maximum titre of 48.5±0.2 g/L xylonic acid from 60 g/L xylose derived from biomass was obtained with a yield of 0.89 g/g xylose which corresponds to a conversion of 62%.30.8±0.4 g/L of xylonic acid crystals of 95.5%purity were successfully recovered from 1L sawdust APL(Acid Pre-treated Liquor)via solvent precipitation and crystallization.Purified xylonic acid crystals were checked for its antibacterial effect on Salmonella enterica MTCC 3224,Escherichia coli MTCC 443 and Staphylococcus aureus MTCC 96.
基金The work was supported by the grant from the Department of Science and Technology-SERB,Government of India through the Core Research Grant Project“CRG/2019/001913:Investigation on identifcation and biochemical validation of selenoproteins from Nannochloropsis oceanica CASA CC201 as functional food/feed supplements”to MA.We are thankful for the fnancial support from DST-INSPIRE fellowship of Department of Science and Technology,New Delhi for doctoral studies of AU.
文摘Microalgae are considered a rich source of high-value metabolites with an array of nutraceutical and pharmaceutical applications.Diferent strategies have been developed for cultivating microalgae at large-scale photobioreactors but high cost and low productivity are the major hurdles.Optimizing the composition of media for the cultivation of microalgae to induce biomass production and high-value metabolite accumulation has been considered as an important factor for sustainable product development.In this study,the efect of plant growth regulators together with basal microalgal cultivation medium on biomass,total lipid,and EPA production was studied using the Plackett–Burman model and Response surface methodology.The traditional one-factor-at-a-time optimization approach is laborious,time-consuming,and requires more experiments which makes the process and analysis more difcult.The Designed PB model was found to be signifcant for biomass(396 mg/L),lipid(254 mg/L),and EPA(5.6%)production with a P value<0.05.The major objective of this study is to formulate a medium for EPA production without compromising the growth properties.Further,we had formulated a new media using RSM to achieve the goal and the signifcant variables selected were NaNO_(3),NaH_(2)PO_(4),and IAA and was found to be signifcant with 16.72%EPA production with a biomass production of 893 mg/L with a P value<0.05.The formulated medium can be used in large-scale cultivation systems which can enhance biomass production as well as the omega 3 fatty acid production in marine microalgae Nannochloropsis oceanica.
文摘Hydrothermal liquefaction(HTL)and pyrolysis(Py)of Loktak lake biomass mixture of water hyacinth(WH)and para grass(PG)were carried out at 260-300℃ and 300-500℃ to compare the products yield and chemical characteristics of the products.In case of HTL,the maximum bio-oil yield was obtained 13.34 wt.%at 280℃ while for Py,the maximum bio-oil yield was observed 38.8 wt.%at 350℃.The obtained bio-oils and bio-chars were analyzed using GC-MS,FT-IR,NMR,TGA,TOC,and SEM.GC-MS analysis of the bio-oils were showed that the HTL bio-oil majorly contains of nitrogen containing compounds whereas the Py bio-oil contains majority of phenolic compounds.Other compounds like ketones,alcohols,acids were also observed in bio-oil.Higher intensity broad band at 3300-3500 cm^(−1) was observed in the Py bio-oil compared to HTL bio-oil.TGA and proximate analysis of bio-char revealed the higher devolatilization occured during the HTL compared to Py process.The surface morphology of the HTL bio-char was found to be rough and fragmented as compared to the pyrolysis bio-char,clearly showing the biomass macromolecules breakdown differently in HTL and Py process.
基金Research(CSIR)for the financial support for the project MLP 0035(33/2018/MD-FTT&FTC-ANB)of which this study forms a part of.
文摘Byssochlamys fulva AM130,a novel strain of filamentous fungus,could produce ethanol from glucose,xylose,and alkali pretreated rice straw(PRS),while the efficiencies were very low with PRS.Ethanol production of 11.84 g/L was attained by the fungus when grown in glucose,indicating that the limitations while growing on PRS were related to low hydrolytic efficiency.Enzyme profiling of the fungus showed 365 IU/ml of beta-glucosidase and 89 IU/ml of xylanase activity,while endoglucanase and filter paper activity were negligible,which accounts for the low hydrolytic efficiency.The fungus could survive for extended periods under oxygen-limited conditions and produce ethanol.The fungal mycelia could also be used for repeated cycles of anaerobic fermentation,wherein the ethanol yield improved with each consecutive cycle.
文摘Problems with food security result from increased population,global warming,and decrease in cultivable land.With the advancements in synthetic biology,microbial synthesis of food is considered to be an efficient alternate approach that could permit quick food biosynthesis in an eco-friendly method.Furthermore,synthetic biology can be assumed to the synthesis of healthy or specially designed food components like proteins,lipids,amino acids and vitamins and widen the consumption of feedstocks,thus offering possible resolutions to high-quality food synthesis.This review describes the impact of synthetic biology for the microbial synthesis of various food ingredients production.
