Background:During the establishment of a model of acute kidney injury(AKI)in pigs,we observed a high prevalence of malignant hyperthermia(MH).These complications led us to refine the anesthetic protocol.This publicati...Background:During the establishment of a model of acute kidney injury(AKI)in pigs,we observed a high prevalence of malignant hyperthermia(MH).These complications led us to refine the anesthetic protocol.This publication describes the impact of the choice of anesthetics on the results obtained.Methods:Pigs were euthanized at the end of the procedure,without recovery from anesthesia.Three anesthetic protocols were used:sevoflurane inhalation(ProtocolA,n=5),a combination of ketamine,medetomidine and diazepam by intravenous infusion(ProtocolB,n=5),and a combination of ketamine,diazepam,medetomidine,glucose,and noradrenaline(ProtocolC,n=5).All pigs received morphine for analgesia.AKI was induced by interrupting renal perfusion for 90 min.MH was diagnosed based on clinical and biological parameters.Results:All MH pigs belonged to ProtocolA.MH pigs showed significantly higher maximum rectal temperature(p=0.04),maximum expired carbon dioxide(CO_(2);p=0.04),maximum heart rate(HR;p=0.03),plasma concentration of creatinine and potassium(p<0.0001).Protocol A pigs had a significantly higher maximum HR(p=0.01)and hyperkalemia compared to the two other groups(ProtocolB,p=0.005 and ProtocolC,p<0.0001).Pigs from ProtocolA had a significantly lower minimum mean arterial pressure(MAP)than ProtocolC group(p=0.03)and MAP remained below 60 mmHg for longer(p=0.004).In ProtocolB,minimum glycemia was lower than other groups(p=0.01).Conclusion:Sevoflurane use was associated with the occurrence of MH,hemodynamic alterations and changes in plasma concentration of creatinine and potassium.These modifications can have a major impact on the validation of an experimental AKI model.展开更多
文摘Background:During the establishment of a model of acute kidney injury(AKI)in pigs,we observed a high prevalence of malignant hyperthermia(MH).These complications led us to refine the anesthetic protocol.This publication describes the impact of the choice of anesthetics on the results obtained.Methods:Pigs were euthanized at the end of the procedure,without recovery from anesthesia.Three anesthetic protocols were used:sevoflurane inhalation(ProtocolA,n=5),a combination of ketamine,medetomidine and diazepam by intravenous infusion(ProtocolB,n=5),and a combination of ketamine,diazepam,medetomidine,glucose,and noradrenaline(ProtocolC,n=5).All pigs received morphine for analgesia.AKI was induced by interrupting renal perfusion for 90 min.MH was diagnosed based on clinical and biological parameters.Results:All MH pigs belonged to ProtocolA.MH pigs showed significantly higher maximum rectal temperature(p=0.04),maximum expired carbon dioxide(CO_(2);p=0.04),maximum heart rate(HR;p=0.03),plasma concentration of creatinine and potassium(p<0.0001).Protocol A pigs had a significantly higher maximum HR(p=0.01)and hyperkalemia compared to the two other groups(ProtocolB,p=0.005 and ProtocolC,p<0.0001).Pigs from ProtocolA had a significantly lower minimum mean arterial pressure(MAP)than ProtocolC group(p=0.03)and MAP remained below 60 mmHg for longer(p=0.004).In ProtocolB,minimum glycemia was lower than other groups(p=0.01).Conclusion:Sevoflurane use was associated with the occurrence of MH,hemodynamic alterations and changes in plasma concentration of creatinine and potassium.These modifications can have a major impact on the validation of an experimental AKI model.
