In order To evaluate whether the parameters of spiral artery blood flow, as measured by transvaginal color Doppler, may be used to assess endometrium receptivity prior to embryo transfer (ET), a retrospective study of...In order To evaluate whether the parameters of spiral artery blood flow, as measured by transvaginal color Doppler, may be used to assess endometrium receptivity prior to embryo transfer (ET), a retrospective study of 94 infertile women who had undergone ART treatments with different outcomes (pregnant or nonpregnant) was done. Subendometrial blood flow was evaluated. The resistance index (RI), systolic/diastolic ratio (S/D) and pulsatility index (PI) were significantly lower in those who achieved pregnancy as compared with those who did not: 0.62±0.04 vs 0.68±0.04 (P<0.001), 2.66±0.33 vs 3.19±0.39 (P<0.01) and 1.15±0.17 vs 1.34±0.22 (P<0.05), respectively. Furthermore, when RI>0.72, PI>1.6, and S/D>3.6, no pregnancy occurred. These data suggest that the parameters of spiral artery blood flow could be used as a new assay in predicting endometrial receptivity before ET.展开更多
Objective To explore the association of genetic polymorphisms in the genes encoding the anti-Miillerian hormone （AMH） and its type H receptor （AMHRII） with ovarian hyperstimulation syndrome （OHSS）. Methods Using...Objective To explore the association of genetic polymorphisms in the genes encoding the anti-Miillerian hormone （AMH） and its type H receptor （AMHRII） with ovarian hyperstimulation syndrome （OHSS）. Methods Using polymerase chain reaction （PCR） and DNA sequencing techniques, the exons of AMH and AMHRII were analyzed in 27 OHSS patients （OHSS group） and 22 non-OHSS patients （control group） who were applied controlled ovarian hyper- stimulation （COH）. Single nucleotide polymorphisms （SNPs） were also analyzed. Results SNPs G〉 T at position 146 of AMH exon 1 and G〉 A at position 134 of AMH exon 2 showed significant differences between the OHSS group and control group （P〈0.05）. SNP G〉 T at position 303 of AMH exon 1 showed no significant difference between the OHSS group and control group （P〉0.05）. No SNP was detected from the AMHR H exons 1 to 11 in either groups. Conclusion Genetic polymorphisms in the AMH gene may be a cause of ovarian hypersensitivity to exogenous hormone stimulation and the development of OHSS.展开更多
Sildenafil is primarily used in the treatment of erectile dysfunction because of its ability to enhance nitric oxide-mediated relaxation in the corpus cavernosum.Recent studies have highlighted its potential anti-canc...Sildenafil is primarily used in the treatment of erectile dysfunction because of its ability to enhance nitric oxide-mediated relaxation in the corpus cavernosum.Recent studies have highlighted its potential anti-cancer effects in various cancer types.Simultaneously,concerns have been raised about its possible pro-cancer activity,with multiple studies suggesting a link between sildenafil use and an increased risk of melanoma.Although this causal link remains uncertain,the dual oncological effects of this drug require careful consideration in clinical applications.This mini-review aims to explore the current evidence on the anti-cancer and pro-cancer effects of sildenafil,offering insights into its judicious use in Oncology.展开更多
Objectives:Polycystic ovary syndrome(PCOS)is a common endocrine disease in women of childbearing age.Although it is a leading cause of menstrual disorders,infertility,obesity,and other diseases,its molecular mechanism...Objectives:Polycystic ovary syndrome(PCOS)is a common endocrine disease in women of childbearing age.Although it is a leading cause of menstrual disorders,infertility,obesity,and other diseases,its molecular mechanism remains unclear.This study aimed to analyze the target genes,pathways,and potential drugs for PCOS through text mining.Methods:First,three different keywords("polycystic ovary syndrome","obesity/adiposis",and"anovulation")were uploaded to GenCLiP3 to obtain three different gene sets.We then chose the common genes among these gene sets.Second,we performed gene ontology and signal pathway enrichment analyses of these common genes,followed by protein-protein interaction(PPI)network analysis.Third,the most significant gene module clustered in the protein-protein network was selected to identify potential drugs for PCOS via gene-drug analysis.Results:A total of 4291 genes related to three different keywords were obtained through text mining,72 common genes were filtered among the three gene sets,and 69 genes participated in PPI network construction,of which 23 genes were clustered in the gene modules.Finally,six of the 23 genes were targeted by 30 existing drugs.Conclusions:The discovery of the six genes(CYP19A1,ESR1,IGF1R,PGR,PTGS2,and VEGFA)and 30 targeted drugs,which are associated with ovarian steroidogenesis(P<0.001),may be used in potential therapeutic strategies for PCOS.展开更多
Objective:Polycystic ovarian syndrome(PCOS)is a common endocrine disorder affecting women of reproductive age.This study aimed to use text mining and microarray data analysis to identify drugs that target genes and po...Objective:Polycystic ovarian syndrome(PCOS)is a common endocrine disorder affecting women of reproductive age.This study aimed to use text mining and microarray data analysis to identify drugs that target genes and potential pathways associated with PCOS.Methods:We extracted a common set of genes associated with PCOS using text mining and the microarray dataset GSE48301.Next,we performed Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses of these genes,as well as protein–protein interaction(PPI)network analysis.Additionally,we used MCODE and cytoHubba to cluster significant common genes in the PPI network and performed gene–drug interaction analyses to identify potential drugs for further investigation.Finally,we annotated pathways associated with the genes identified.Results:Text mining and microarray analysis yielded 696 text mining genes(TMGs)and 2804 differentially expressed genes(DEGs).Among these,a set of 77 genes was found in both TMGs and DEGs.Interestingly,67 of these genes participated in constructing the PPI network.Seven common hub genes were selected using the MCODE and CytoHubba methods.Finally,five out of seven genes were targeted by 15 existing drugs.Conclusion:Four genes(FASLG,IL13,IL17A,andIL2RA),which are mainly related to the cytokine–cytokine receptor interaction pathway,could be prioritized as targets for PCOS.展开更多
Objective:Polycystic ovary syndrome(PCOS)is an endocrine disorder with diverse clinical manifestations that often occurs in women of childbearing age.However,its molecular pathogenesis remains unclear,and this study a...Objective:Polycystic ovary syndrome(PCOS)is an endocrine disorder with diverse clinical manifestations that often occurs in women of childbearing age.However,its molecular pathogenesis remains unclear,and this study aimed to identify miRNA targets in PCOS through text mining and database analysis.Methods:First,three different sets of text mining genes(TMGs)associated with"polycystic ovary syndrome","obesity/adiposis",and"anovulation"keywords were retrieved from the GenCLiP3 database,and overlapping genes were selected.Second,Gene ontology annotation and biological pathway enrichment analyses of these overlapping TMGs were performed,followed by protein-protein interaction(PPI)network analysis.Third,genes in the gene module clustered in the PPI were selected to predict potential miRNAs for PCOS via miRNA-mRNA analysis.Results:A total of 4291 TMGs related to three different keywords were obtained through text mining;72 intersect TMGs were retained among the three gene sets,and 62 TMGs participated in the establishment of the PPI network,of which 18 were aggregated in the gene module.Finally,11 miRNAs that simultaneously bound to two TMGs(IGF1,ESR1,MAPK1,NAMPT,PIK3CA,and SERPINE1)could be prioritized as targets to study PCOS.Conclusion(s):The discovery of 11 miRNAs(miR-301a-3p,miR-301b-3p,miR-3666,miR-454-3p,miR-130a-3p,miR-130b-3p,miR-4295,miR-190a-3p,miR-5011-5p,miR-548c-3p,and miR-4799-5p)and 6 TMGs,which are associated with the HIF-1 signaling pathway(P=4.799E-08),could be used as potential targets for PCOS.展开更多
文摘In order To evaluate whether the parameters of spiral artery blood flow, as measured by transvaginal color Doppler, may be used to assess endometrium receptivity prior to embryo transfer (ET), a retrospective study of 94 infertile women who had undergone ART treatments with different outcomes (pregnant or nonpregnant) was done. Subendometrial blood flow was evaluated. The resistance index (RI), systolic/diastolic ratio (S/D) and pulsatility index (PI) were significantly lower in those who achieved pregnancy as compared with those who did not: 0.62±0.04 vs 0.68±0.04 (P<0.001), 2.66±0.33 vs 3.19±0.39 (P<0.01) and 1.15±0.17 vs 1.34±0.22 (P<0.05), respectively. Furthermore, when RI>0.72, PI>1.6, and S/D>3.6, no pregnancy occurred. These data suggest that the parameters of spiral artery blood flow could be used as a new assay in predicting endometrial receptivity before ET.
基金supported by a scientific research grant from Tongji Hospital of Tongji Medical College of Huazhong University of Science and Technologythe National Natural Science Fund (Project No. 81200474)
文摘Objective To explore the association of genetic polymorphisms in the genes encoding the anti-Miillerian hormone （AMH） and its type H receptor （AMHRII） with ovarian hyperstimulation syndrome （OHSS）. Methods Using polymerase chain reaction （PCR） and DNA sequencing techniques, the exons of AMH and AMHRII were analyzed in 27 OHSS patients （OHSS group） and 22 non-OHSS patients （control group） who were applied controlled ovarian hyper- stimulation （COH）. Single nucleotide polymorphisms （SNPs） were also analyzed. Results SNPs G〉 T at position 146 of AMH exon 1 and G〉 A at position 134 of AMH exon 2 showed significant differences between the OHSS group and control group （P〈0.05）. SNP G〉 T at position 303 of AMH exon 1 showed no significant difference between the OHSS group and control group （P〉0.05）. No SNP was detected from the AMHR H exons 1 to 11 in either groups. Conclusion Genetic polymorphisms in the AMH gene may be a cause of ovarian hypersensitivity to exogenous hormone stimulation and the development of OHSS.
基金Jiuquan Science and Technology Livelihood Program Project Class A(2023MA3047)Jiuquan City People’s Livelihood Science and Technology Plan Project Class B(2024MB1054)+2 种基金Natural Science Foundation of Fujian Province(2022J05298)Xiamen Medical and Health Guidance Project,China(3502Z20244ZD1057)Natural Science Foundation of Xiamen,China(3502Z20227155)。
文摘Sildenafil is primarily used in the treatment of erectile dysfunction because of its ability to enhance nitric oxide-mediated relaxation in the corpus cavernosum.Recent studies have highlighted its potential anti-cancer effects in various cancer types.Simultaneously,concerns have been raised about its possible pro-cancer activity,with multiple studies suggesting a link between sildenafil use and an increased risk of melanoma.Although this causal link remains uncertain,the dual oncological effects of this drug require careful consideration in clinical applications.This mini-review aims to explore the current evidence on the anti-cancer and pro-cancer effects of sildenafil,offering insights into its judicious use in Oncology.
基金supported by the self-funded Scientific Research Project of Jiuquan City People’s Hospital,Gansu,China.
文摘Objectives:Polycystic ovary syndrome(PCOS)is a common endocrine disease in women of childbearing age.Although it is a leading cause of menstrual disorders,infertility,obesity,and other diseases,its molecular mechanism remains unclear.This study aimed to analyze the target genes,pathways,and potential drugs for PCOS through text mining.Methods:First,three different keywords("polycystic ovary syndrome","obesity/adiposis",and"anovulation")were uploaded to GenCLiP3 to obtain three different gene sets.We then chose the common genes among these gene sets.Second,we performed gene ontology and signal pathway enrichment analyses of these common genes,followed by protein-protein interaction(PPI)network analysis.Third,the most significant gene module clustered in the protein-protein network was selected to identify potential drugs for PCOS via gene-drug analysis.Results:A total of 4291 genes related to three different keywords were obtained through text mining,72 common genes were filtered among the three gene sets,and 69 genes participated in PPI network construction,of which 23 genes were clustered in the gene modules.Finally,six of the 23 genes were targeted by 30 existing drugs.Conclusions:The discovery of the six genes(CYP19A1,ESR1,IGF1R,PGR,PTGS2,and VEGFA)and 30 targeted drugs,which are associated with ovarian steroidogenesis(P<0.001),may be used in potential therapeutic strategies for PCOS.
文摘Objective:Polycystic ovarian syndrome(PCOS)is a common endocrine disorder affecting women of reproductive age.This study aimed to use text mining and microarray data analysis to identify drugs that target genes and potential pathways associated with PCOS.Methods:We extracted a common set of genes associated with PCOS using text mining and the microarray dataset GSE48301.Next,we performed Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses of these genes,as well as protein–protein interaction(PPI)network analysis.Additionally,we used MCODE and cytoHubba to cluster significant common genes in the PPI network and performed gene–drug interaction analyses to identify potential drugs for further investigation.Finally,we annotated pathways associated with the genes identified.Results:Text mining and microarray analysis yielded 696 text mining genes(TMGs)and 2804 differentially expressed genes(DEGs).Among these,a set of 77 genes was found in both TMGs and DEGs.Interestingly,67 of these genes participated in constructing the PPI network.Seven common hub genes were selected using the MCODE and CytoHubba methods.Finally,five out of seven genes were targeted by 15 existing drugs.Conclusion:Four genes(FASLG,IL13,IL17A,andIL2RA),which are mainly related to the cytokine–cytokine receptor interaction pathway,could be prioritized as targets for PCOS.
基金supported by the self-funded Scientific Research Project of People’s Hospital of Jiuquan City,Gansu,China and the self-funded Scientific Research Project of Xiamen Health and Medical Big Data Center,Xiamen,China.
文摘Objective:Polycystic ovary syndrome(PCOS)is an endocrine disorder with diverse clinical manifestations that often occurs in women of childbearing age.However,its molecular pathogenesis remains unclear,and this study aimed to identify miRNA targets in PCOS through text mining and database analysis.Methods:First,three different sets of text mining genes(TMGs)associated with"polycystic ovary syndrome","obesity/adiposis",and"anovulation"keywords were retrieved from the GenCLiP3 database,and overlapping genes were selected.Second,Gene ontology annotation and biological pathway enrichment analyses of these overlapping TMGs were performed,followed by protein-protein interaction(PPI)network analysis.Third,genes in the gene module clustered in the PPI were selected to predict potential miRNAs for PCOS via miRNA-mRNA analysis.Results:A total of 4291 TMGs related to three different keywords were obtained through text mining;72 intersect TMGs were retained among the three gene sets,and 62 TMGs participated in the establishment of the PPI network,of which 18 were aggregated in the gene module.Finally,11 miRNAs that simultaneously bound to two TMGs(IGF1,ESR1,MAPK1,NAMPT,PIK3CA,and SERPINE1)could be prioritized as targets to study PCOS.Conclusion(s):The discovery of 11 miRNAs(miR-301a-3p,miR-301b-3p,miR-3666,miR-454-3p,miR-130a-3p,miR-130b-3p,miR-4295,miR-190a-3p,miR-5011-5p,miR-548c-3p,and miR-4799-5p)and 6 TMGs,which are associated with the HIF-1 signaling pathway(P=4.799E-08),could be used as potential targets for PCOS.
