The SCC (stress corrosion cracking) susceptibility of steel l6Mn in nitratesolution was studied. The results showed that applied potential polarization would accelerate(anodic polarization) or retard (cathodic polariz...The SCC (stress corrosion cracking) susceptibility of steel l6Mn in nitratesolution was studied. The results showed that applied potential polarization would accelerate(anodic polarization) or retard (cathodic polarization) the SCC process. The study on phaseelectrochemistry revealed that there was significant difference in electrochemical performancebetween ferrite and pearlite of steel 16Mn. Pearlite preferentially corroded under the action ofgalvanic cell. The observation on time and in situ showed that corrosion started first at the phaseboundary between ferrite and pearlite, and the pearlite gradually corroded until disappeared, andthen corrosion crossed the phase boundary extending into the ferrite phase. According to this, ananodic dissolution mechanism of SCC was proposed, on which pre-existing sting active path and phaseelectrochemistry (PEAP-PEC) jointly came into action (SCC mechanism of PEAP-PEC).展开更多
The physiological markers of 310 individuals aged 2 through 19 were evaluated for the effects of the Masgutova Neurosensorimotor Reflex Integration Method on their four body systems: respiratory, cardiovascular, diges...The physiological markers of 310 individuals aged 2 through 19 were evaluated for the effects of the Masgutova Neurosensorimotor Reflex Integration Method on their four body systems: respiratory, cardiovascular, digestive, and nervous systems of individuals with neurodevelopmental deficits—cerebral palsy (CP), seizures, traumatic and acute brain injury, attention deficit and hyperactive disorders (ADD, ADHD), autism spectrum disorders, anxiety, post-trauma and post-traumatic stress disorders. We found that 53.33% of physiological markers and 66.67% of reflex patterns on the pre-test demonstrated to be poorly functioning. Both evaluation results showed statistically significant improvements after 8-days of intensive training using the Masgutova Neurosensorimotor Reflex Integration Method. Improvements according to 60.0% of the physiological markers positively correlated with functionality gains in 77.5% of reflex patterns in all four study groups compared to the control group, which did not receive the Reflex Integration training program (p-value < 0.05). The magnitude of improvement depended upon the severity of symptoms indicating the essentiality for individualized training in accordance with the diagnosis and individual neurological deficits. Results of this study show that reflex integrative techniques can lead to a reduction of stress and other negative factors blocking health homeostasis, limiting perception, and causing dysregulation in behavior and emotions, especially following traumatic events. Positive changes in physiological markers and reflex pattern functions indicate potential benefits for survival and stress resiliency through supporting neuro-physiological and neuro-psychological aspects of overall health and well-being in individuals with neurological deficits.展开更多
Introduction: The MNRI (Masgutova Neurosensorimotor Reflex Integration) method was developed in 1989 in Russia and has spread world-wide to treat individuals with certain types reflex development deficits, behavior di...Introduction: The MNRI (Masgutova Neurosensorimotor Reflex Integration) method was developed in 1989 in Russia and has spread world-wide to treat individuals with certain types reflex development deficits, behavior disorders, disorders of speech or language development, and learning disabilities. MNRI is based on techniques called “repatterning” or remodulation, meaning re-education, recoding the reflex nerve pathways specific for dynamic and postural reflex schemes. Objectives: Repatterning activates the extra pyramidal nervous system responsible for automatic mechanisms and processes, the extension of links between neurons, the growth of neural nets, myelination, and the creation of new nerve routing. This potential result was tested utilizing urinary measurements of the following neurotransmitters: epinephrine, norepinephrine, dopamine, DOPAC, serotonin, 5-HIAA, glycine, taurine, GABA, glutamate, PEA, and histamine. Methods: Neurological impact of the Masgutova Neurosensorimotor Reflex Method on the magnitude of changes in neurotransmitters was assessed by an external controlled and double-blind method using patients from one of the four diagnosis groups: 1) global developmental disorders;2) cerebral palsy, Traumatic Brain Injury (TBI), Acute Brain Injury (ABI), and seizures;3) ADD/ADHD;and 4) anxiety disorders. Results: The post-MNRI results in participants show a tendency for regulation of the above neurotransmitters resulting in their calming down, decrease of hypervigilance, stress resilience increase, improvements in behavioral and emotional regulation, positive emotions, and cognitive processes control. Conclusion: The application of the Masgutova Neurosensorimotor Reflex Method as a therapy modality offers a novelty paradigm for the treatment using neuro- and immune-modulation technologies presenting a non-pharmaceutical approach, based on use of neurosensorimotor reflex circuit concept.展开更多
This research reports on potentiality in the solar chimney as an exhaust ventilation device through a hypothetical statement that convenient weather conditions exist on sub-humid warm climate to enhance the performanc...This research reports on potentiality in the solar chimney as an exhaust ventilation device through a hypothetical statement that convenient weather conditions exist on sub-humid warm climate to enhance the performance ofa SC （solar chimney）, in order to increase ventilation to dissipate metabolic heat from inhabitants of airtight buildings. The methodology used in this research integrates the use of simplified mathematical models that predict in a semi-empirical way a potential volume flow through estimation of kinetic power generation, by natural circulation and stack effect in a typical covered-plate air solar collector. The study was carried out during the warm month of April, an important warm season for Colima, where the prevailing wind is about 6.8 m/s, the average temperature of maximums and minimums oscillates between 15.4℃and 34.4 ℃ with average relative humidity of 65% according to national weather service. A scale model of the SC was installed on environmental chambers. The results showed that this device is capable of generating ventilation conditions around light breeze parameters near between 0.6 m/s and 1.5 m/s width, a better performance at day in 45% approximately than the night values, making ventilation rates up 0.24 ACH （air changes per hour） as average air change rate.展开更多
Background: Immune dysfunction and a higher risk of uterine infections are characteristics of the transition into lactation in dairy cows. The supply of complexed trace minerals, which are more bioavailable, could hel...Background: Immune dysfunction and a higher risk of uterine infections are characteristics of the transition into lactation in dairy cows. The supply of complexed trace minerals, which are more bioavailable, could help overcome the greater needs of these nutrients in tissues around parturition and early lactation.Results: Twenty Holstein cows received an oral bolus with a mix of inorganic trace minerals(INO) or complexed trace minerals(AAC) to achieve 75, 65, 11, and 1 ppm supplemental Zn, Mn, Cu, and Co, respectively, in the total diet dry matter from -30 d through +30 d relative to parturition. Blood for polymorphonuclear leukocyte(PMNL) isolation was collected at-30,-15, +10, and + 30 d relative to parturition, whereas endometrium biopsies were performed at +14 and +30 d. Feeding AAC led to greater PMNL expression of genes related with inflammation response(DDX58), oxidative stress response(MPO), eicosanoid metabolism(PLA2G4A and ALOX5AP), transcription regulation(PPARG), and cellular adhesion(TLN1). The upregulation by AAC in endometrium of genes related with inflammation response( TLR2, TLR4, NFKB1, TNF, IL6, IL1 B, IL10, IL8), prostaglandin synthesis(PTGS2, PTGES), and antioxidant responses(NFE2 L2, SOD1) indicated a faster remodeling of uterine tissue and potentially greater capacity to control a local bacterial invasion.Conclusions: Data indicate that trace mineral supplementation from amino acid complexes improves PMNL activity and allows the prompt recovery of uterine tissue during early lactation. As such, the benefits of complexed trace minerals extend beyond an improvement of liver function and productive performance.展开更多
Objective.To develop a 3D shear wave elastography(SWE)technique using a 2D row column addressing(RCA)array,with either external vibration or acoustic radiation force(ARF)as the shear wave source.Impact Statement.The p...Objective.To develop a 3D shear wave elastography(SWE)technique using a 2D row column addressing(RCA)array,with either external vibration or acoustic radiation force(ARF)as the shear wave source.Impact Statement.The proposed method paves the way for clinical translation of 3D SWE based on the 2D RCA,providing a low-cost and high volume rate solution that is compatible with existing clinical systems.Introduction.SWE is an established ultrasound imaging modality that provides a direct and quantitative assessment of tissue stiffness,which is significant for a wide range of clinical applications including cancer and liver fibrosis.SWE requires high frame rate imaging for robust shear wave tracking.Due to the technical challenges associated with high volume rate imaging in 3D,current SWE techniques are typically confined to 2D.Advancing SWE from 2D to 3D is significant because of the heterogeneous nature of tissue,which demands 3D imaging for accurate and comprehensive evaluation.Methods.A 3D SWE method using a RCA array was developed with a volume rate up to 2000 Hz.The performance of the proposed method was systematically evaluated on tissue-mimicking elasticity phantoms and in an in vivo case study.Results.3D shear wave motion induced by either external vibration or ARF was successfully detected with the proposed method.Robust 3D shear wave speed maps were reconstructed for phantoms and in vivo.Conclusion.The high volume rate 3D imaging provided by the 2D RCA array provides a robust and practical solution for 3D SWE with a clear pathway for future clinical translation.展开更多
Flow diverting stents are braided,metallic endoluminal devices widely used to treat intracranial aneurysms.Bioresorbable flow diverters(BRFDs)are gaining traction as the next generation of flow diverter technology.BRF...Flow diverting stents are braided,metallic endoluminal devices widely used to treat intracranial aneurysms.Bioresorbable flow diverters(BRFDs)are gaining traction as the next generation of flow diverter technology.BRFDs aim to occlude and heal the aneurysm before safely dissolving into the body,mitigating or eliminating complications associated with the permanent presence of conventional flow diverters such as thromboembolism and stenosis.Additional putative advantages of a BRFD include a reduction in metal induced medical imaging artifacts,a restoration of physiological vasoreactivity,and allowing physicians to re-access the aneurysm if an additional procedure is required.In this current study,iron-manganese-nitrogen(FeMnN)alloy BRFDs and permanent control FDs composed of an industry standard Cobalt-Nickel-Chromium alloy were deployed in the rabbit aorta.MicroCT and SEM corrosion analysis determined the FeMnN wire volumes and cross-sectional areas had reduced approximately 85%and 95%after 3-and 6-months implantation duration,respectively.Histo-logical analysis demonstrated the BRFDs exhibited suitable biocompatibility,with no cases of in-stent throm-bosis,clinically significant stenosis,or adverse tissue responses observed.Immunohistochemistry revealed the neointimas surrounding the BRFDs featured a confluent endothelium covering several layers of smooth muscle cells,with macrophages adjacent to the device wires.The macrophages were able to penetrate the corrosion product and were observed transporting corrosion products away from the implant site.This current work provides primary in vivo corrosion and biocompatibility data to the field for FeMn alloys,which we feel will stimulate and inform the design of next-generation bioresorbable endovascular devices.展开更多
The unfolded protein response is a cellular pathway activated to maintain proteostasis and prevent cell death when the endoplasmic reticulum is overwhelmed by unfolded proteins.However,if the unfolded protein response...The unfolded protein response is a cellular pathway activated to maintain proteostasis and prevent cell death when the endoplasmic reticulum is overwhelmed by unfolded proteins.However,if the unfolded protein response fails to restore endoplasmic reticulum homeostasis,it can trigger proinflammatory and pro-death signals,which are implicated in various malignancies and are currently being investigated for their role in retinal degenerative diseases.This paper reviews the role of the unfolded protein responsein addressing endoplasmic reticulumstress in retinal degenerative diseases.The accumulation of ubiquitylated misfolded proteins can lead to rapid destabilization of the proteome and cellular demise.Targeting endoplasmic reticulum stress to alleviate retinal pathologies involves multiple strategies,including the use of chemical chaperones such as 4-phenylbutyric acid and tauroursodeoxycholic acid,which enhance protein folding and reduce endoplasmic reticulum stress.Small molecule modulators that influence endoplasmic reticulum stress sensors,including those that increase the expression of the endoplasmic reticulum stress regulator X-box binding protein 1,are also potential therapeutic agents.Additionally,inhibitors of the RNAse activity of inositol-requiring transmembrane kinase/endoribonuclease 1,a key endoplasmic reticulum stress sensor,represent another class of drugs that could prevent the formation of toxic aggregates.The activation of nuclear receptors,such as PPAR and FXR,may also help mitigate ER stress.Furthermore,enhancing proteolysis through the induction of autophagy or the inhibition of deubiquitinating enzymes can assist in clearing misfolded proteins.Combination treatments that involve endoplasmicreticulum-stress-targeting drugs and gene therapies are also being explored.Despite these potential therapeutic strategies,significant challenges remain in targeting endoplasmic reticulum stress for the treatment of retinal degeneration,and further research is essential to elucidate the mechanisms underlying human retinal diseases and to develop effective,well-tolerated drugs.The use of existing drugs that target inositol-requiring transmembrane kinase/endoribonuclease 1 and X-box binding protein 1 has been associated with adverse side effects,which have hindered their clinical translation.Moreover,signaling pathways downstream of endoplasmic reticulum stress sensors can contribute to therapy resistance.Addressing these limitations is crucial for developing drugs that can be effectively used in treating retinal dystrophies.In conclusion,while the unfolded protein response is a promising therapeutic target in retinal degenerative diseases,additional research and development efforts are imperative to overcome the current limitations and improve patient outcomes.展开更多
Aims Theory predicts that the success of introduced species is related to the diversity of native species through trait-based processes.Abiotic site characteristics may also affect a site’s susceptibility to invasion...Aims Theory predicts that the success of introduced species is related to the diversity of native species through trait-based processes.Abiotic site characteristics may also affect a site’s susceptibility to invasion.We quantified resident plant species richness,phylogenetic diversity and several abiotic site characteristics for 24 oak forests in Minnesota,USA,to assess their impact on the abundance of a widespread,introduced terrestrial plant species,common buckthorn(Rhamnus cathartica L.).Specifically,we asked(1)whether resident species richness and phylogenetic diversity affected the abundance of R.cathartica and(2)what site characteristics explained the overall abundance of R.cathartica.Methods Our survey included 24 oak-dominated stands in Minnesota’s deciduous forests.In each stand,we identified all species in 16 plots.We also measured a series of environmental site characteristics,including canopy openness(a proxy for light availability),percent bare soil,soil pH,percent sand,an index of propagule availability,duff layer thickness(a proxy for earthworm activity),an index of insolation and slope.For all species present in at least one site,we estimated a community phylogeny.We combined all sitelevel characteristics,including phylogenetic diversity of the resident plant species,in a multiple regression model to examine site-level drivers of community invasibility.Important Findings Results indicate that sites with higher overall plant phylogenetic diversity harbor less R.cathartica,even though native species richness was not significantly related to R.cathartica abundance.Regression analyses indicated that,in addition to resident species phylogenetic diversity,the most important predictors of R.cathartica abundance were canopy openness and the amount of bare soil,both positively related to the abundance of the invader.By combining the effects of abiotic site characteristics and resident species phylogenetic diversity in a model that predicted the abundance of R.cathartica,we were able to simultaneously account for a wide range of factors that might influence invasibility.Overall,our results suggest that management strategies aimed at reducing disturbances that lead to increased bare soil and light levels may be more successful if they also maximize phylogenetic diversity of the resident plant community.展开更多
Flow diverter devices are small stents used to divert blood flow away from aneurysms in the brain,stagnating flow and inducing intra-aneurysmal thrombosis which in time will prevent aneurysm rupture.Current devices ar...Flow diverter devices are small stents used to divert blood flow away from aneurysms in the brain,stagnating flow and inducing intra-aneurysmal thrombosis which in time will prevent aneurysm rupture.Current devices are formed from thin(~25μm)wires which will remain in place long after the aneurysm has been mitigated.As their continued presence could lead to secondary complications,an absorbable flow diverter which dissolves into the body after aneurysm occlusion is desirable.The absorbable metals investigated to date struggle to achieve the necessary combination of strength,elasticity,corrosion rate,fragmentation resistance,radiopacity,and biocompatibility.This work proposes and investigates a new composite wire concept combining absorbable iron alloy(FeMnN)shells with one or more pure molybdenum(Mo)cores.Various wire configurations are produced and drawn to 25–250μm wires.Tensile testing revealed high and tunable mechanical properties on par with existing flow diverter materials.In vitro degradation testing of 100μm wire in DMEM to 7 days indicated progressive corrosion and cracking of the FeMnN shell but not of the Mo,confirming the cathodic protection of the Mo by the FeMnN and thus mitigation of premature fragmentation risk.In vivo implantation and subsequentμCT of the same wires in mouse aortas to 6 months showed meaningful corrosion had begun in the FeMnN shell but not yet in the Mo filament cores.In total,these results indicate that these composites may offer an ideal combination of properties for absorbable flow diverters.展开更多
A small but measurable percentage of major hepatic resection patients will experience major morbidity or mortality due post-hepatectomy liver failure after major liver resection.Pre-operative optimization,in particula...A small but measurable percentage of major hepatic resection patients will experience major morbidity or mortality due post-hepatectomy liver failure after major liver resection.Pre-operative optimization,in particular pre-operative induction of liver growth to increase the future liver remnant(FLR)volume,has become frequently used in patients considered to be high risk.However,this process of hepatic augmentation remains incompletely understood,particularly in humans.Recent advances in techniques for FLR management have resulted in dramatic expansion of the liver surgeon’s armamentarium.展开更多
Some authors have studied a series of (Pd0.9965 Fe0.0035)1-xMnx alloys which exhibit distinctly different magnetic properties depending upon the Mn concentration x. It is attributed to the competing interaction in mag...Some authors have studied a series of (Pd0.9965 Fe0.0035)1-xMnx alloys which exhibit distinctly different magnetic properties depending upon the Mn concentration x. It is attributed to the competing interaction in magnetic systems, In general, for a large number of alloy systems with mixed ferro-antiferromagnetic interactions, the competition leads to a diversity of magnetic struetures and critical phenomena.展开更多
The First UMN-CAS Bilateral Seminar was successfully held in Xi＇an, China, May 27-28,2014. A summary of the 1 st Bilateral Seminar was published in Particuology 16 （2014）, 227-229. The Second UMN-CAS Bilateral Semi...The First UMN-CAS Bilateral Seminar was successfully held in Xi＇an, China, May 27-28,2014. A summary of the 1 st Bilateral Seminar was published in Particuology 16 （2014）, 227-229. The Second UMN-CAS Bilateral Seminar was held at 3M Innovation Center, St. Paul, Minnesota, October 7-8, 2015.展开更多
Photon-counting computed tomography(PCCT)represents a significant advancement in pediatric cardiovascular imaging.Traditional CT systems employ energy-integrating detectors that convert X-ray photons into visible ligh...Photon-counting computed tomography(PCCT)represents a significant advancement in pediatric cardiovascular imaging.Traditional CT systems employ energy-integrating detectors that convert X-ray photons into visible light,whereas PCCT utilizes photon-counting detectors that directly transform X-ray photons into electric signals.This direct conversion allows photon-counting detectors to sort photons into discrete energy levels,thereby enhancing image quality through superior noise reduction,improved spatial and contrast resolution,and reduced artifacts.In pediatric applications,PCCT offers substantial benefits,including lower radiation doses,which may help reduce the risk of malignancy in pediatric patients,with perhaps greater potential to benefit those with repeated exposure from a young age.Enhanced spatial resolution facilitates better visualization of small structures,vital for diagnosing congenital heart defects.Additionally,PCCT’s spectral capabilities improve tissue characterization and enable the creation of virtual monoenergetic images,which enhance soft-tissue contrast and potentially reduce contrast media doses.Initial clinical results indicate that PCCT provides superior image quality and diagnostic accuracy compared to conven-tional CT,particularly in challenging pediatric cardiovascular cases.As PCCT technology matures,further research and standardized protocols will be essential to fully integrate it into pediatric imaging practices,ensuring optimized diagnostic outcomes and patient safety.展开更多
Emerging new races of wheat stem rust(Puccinia graminis f.sp.tritici)are threatening global wheat(Triticum aestivum L.)production.Host resistance is the most effective and environmentally friendly method of controllin...Emerging new races of wheat stem rust(Puccinia graminis f.sp.tritici)are threatening global wheat(Triticum aestivum L.)production.Host resistance is the most effective and environmentally friendly method of controlling stem rust.The stem rust resistance gene Sr59 was previously identified within a T2DS 2RL wheat-rye whole arm translocation,providing broad-spectrum resistance to various stem rust races.Seedling evaluation,molecular marker analysis,and cytogenetic studies identified wheat-rye introgression line#284 containing a new translocation chromosome T2BL 2BS-2RL.This line has demonstrated broad-spectrum resistance to stem rust at the seedling stage.Seedling evaluation and cytogenetic analysis of three backcross populations between the line#284 and the adapted cultivars SLU-Elite,Navruz,and Linkert confirmed that Sr59 is located within the short distal 2RL translocation.This study aimed physical mapping of Sr59 in the 2RL introgression segment and develop a robust molecular marker for marker-assisted selection.Using genotyping-by-sequencing(GBS),GBS-derived SNPs were aligned with full-length annotated rye nucleotide-binding leucine-rich repeat(NLR)genes in the parental lines CS ph1b,SLU238,SLU-Elite,Navruz,and Linkert,as well as in 33 BC4F5progeny.Four NLR genes were identified on the 2R chromosome,with Chr2R_NLR_60 being tightly linked to the Sr59resistance gene.In-silico functional enrichment analysis of the translocated 2RL region(25,681,915 bp)identified 223 genes,with seven candidate genes associated with plant disease resistance and three linked to agronomic performance,contributing to oxidative stress response,protein kinase activity,and cellular homeostasis.These findings facilitate a better understanding of the genetic basis of stem rust resistance provided by Sr59.展开更多
Antimicrobial resistance is a global public health threat,and the World Health Organization(WHO)has announced a priority list of the most threatening pathogens against which novel antibiotics need to be developed.The ...Antimicrobial resistance is a global public health threat,and the World Health Organization(WHO)has announced a priority list of the most threatening pathogens against which novel antibiotics need to be developed.The discovery and introduction of novel antibiotics are time-consuming and expensive.According to WHO’s report of antibacterial agents in clinical development,only 18 novel antibiotics have been approved since 2014.Therefore,novel antibiotics are critically needed.Artificial intelligence(AI)has been rapidly applied to drug development since its recent technical breakthrough and has dramatically improved the efficiency of the discovery of novel antibiotics.Here,we first summarized recently marketed novel antibiotics,and antibiotic candidates in clinical development.In addition,we systematically reviewed the involvement of AI in antibacterial drug development and utilization,including small molecules,antimicrobial peptides,phage therapy,essential oils,as well as resistance mechanism prediction,and antibiotic stewardship.展开更多
BACKGROUND The Warburg effect is common in cancers.Lactate and its receptor GPR81 play an important role in cancer progression.It is widely accepted that membrane receptor nuclear translocation plays some novel role i...BACKGROUND The Warburg effect is common in cancers.Lactate and its receptor GPR81 play an important role in cancer progression.It is widely accepted that membrane receptor nuclear translocation plays some novel role in cancer pathology.The mechanism by which the lactate/GPR81 axis regulates cancer malignancy remains unclear.AIM To elucidate the mechanism of GPR81 nuclear transportation promoted by exogenous lactate.METHODS Lung cancer cells were stimulated with exogenous lactate and GPR81 levels were measured by immunofluoresence and western blot analysis in membrane,cytoplasmic,and nuclear fractions.Lung cancer cells were transduced with a mutant GPR81 nuclear localization signal(NLS)construct,wild type GPR81 or empty vector and used to examine how GPR81 nuclear transportation affects lung cancer cells malignancy in vitro and in vivo.Immunoprecipitation Proteomics analysis and Chromatin immunoprecipitation(ChIP)sequencing were used to determine GPR81 interacting proteins and genes.RESULTS In response to hypoxia/Lactate stimulation,GPR81 translocates and accumulates in the nucleus of lung cancer cells.Functionally,GPR81 nuclear translocation promotes cancer cell proliferation and motility.Depletion of the GPR81 NLS depletes GPR81 nuclear levels and decreases cancer cell growth and invasion in vitro,as well as cancer cell malignancy in vivo.Proteomics analysis revealed a set of proteins including SFPQ,that interact with GPR81 in the cancer cell nucleus.Notably,the interaction of GPR81 with SFPQ promotes cancer cell growth and motility.ChIP sequencing analysis discovered that there is a set of genes targeted by GPR81.CONCLUSION The interaction of GPR81 with SFPQ promotes cancer cell malignancy.GPR81 nuclear translocation is critical in conferring cancer progression and may be a potential therapeutic target for limiting cancer progression.展开更多
There is a growing body of clinical research on the utility of synthetic data derivatives,an emerging research tool in medicine.In nephrology,clinicians can use machine learning and artificial intelligence as powerful...There is a growing body of clinical research on the utility of synthetic data derivatives,an emerging research tool in medicine.In nephrology,clinicians can use machine learning and artificial intelligence as powerful aids in their clinical decision-making while also preserving patient privacy.This is especially important given the epidemiology of chronic kidney disease,renal oncology,and hypertension worldwide.However,there remains a need to create a framework for guidance regarding how to better utilize synthetic data as a practical application in this research.展开更多
基金This work was supported by the National Natural Science Foundation of China (No. 5880009) and the Beijing Natural Science Foun-
文摘The SCC (stress corrosion cracking) susceptibility of steel l6Mn in nitratesolution was studied. The results showed that applied potential polarization would accelerate(anodic polarization) or retard (cathodic polarization) the SCC process. The study on phaseelectrochemistry revealed that there was significant difference in electrochemical performancebetween ferrite and pearlite of steel 16Mn. Pearlite preferentially corroded under the action ofgalvanic cell. The observation on time and in situ showed that corrosion started first at the phaseboundary between ferrite and pearlite, and the pearlite gradually corroded until disappeared, andthen corrosion crossed the phase boundary extending into the ferrite phase. According to this, ananodic dissolution mechanism of SCC was proposed, on which pre-existing sting active path and phaseelectrochemistry (PEAP-PEC) jointly came into action (SCC mechanism of PEAP-PEC).
文摘The physiological markers of 310 individuals aged 2 through 19 were evaluated for the effects of the Masgutova Neurosensorimotor Reflex Integration Method on their four body systems: respiratory, cardiovascular, digestive, and nervous systems of individuals with neurodevelopmental deficits—cerebral palsy (CP), seizures, traumatic and acute brain injury, attention deficit and hyperactive disorders (ADD, ADHD), autism spectrum disorders, anxiety, post-trauma and post-traumatic stress disorders. We found that 53.33% of physiological markers and 66.67% of reflex patterns on the pre-test demonstrated to be poorly functioning. Both evaluation results showed statistically significant improvements after 8-days of intensive training using the Masgutova Neurosensorimotor Reflex Integration Method. Improvements according to 60.0% of the physiological markers positively correlated with functionality gains in 77.5% of reflex patterns in all four study groups compared to the control group, which did not receive the Reflex Integration training program (p-value < 0.05). The magnitude of improvement depended upon the severity of symptoms indicating the essentiality for individualized training in accordance with the diagnosis and individual neurological deficits. Results of this study show that reflex integrative techniques can lead to a reduction of stress and other negative factors blocking health homeostasis, limiting perception, and causing dysregulation in behavior and emotions, especially following traumatic events. Positive changes in physiological markers and reflex pattern functions indicate potential benefits for survival and stress resiliency through supporting neuro-physiological and neuro-psychological aspects of overall health and well-being in individuals with neurological deficits.
文摘Introduction: The MNRI (Masgutova Neurosensorimotor Reflex Integration) method was developed in 1989 in Russia and has spread world-wide to treat individuals with certain types reflex development deficits, behavior disorders, disorders of speech or language development, and learning disabilities. MNRI is based on techniques called “repatterning” or remodulation, meaning re-education, recoding the reflex nerve pathways specific for dynamic and postural reflex schemes. Objectives: Repatterning activates the extra pyramidal nervous system responsible for automatic mechanisms and processes, the extension of links between neurons, the growth of neural nets, myelination, and the creation of new nerve routing. This potential result was tested utilizing urinary measurements of the following neurotransmitters: epinephrine, norepinephrine, dopamine, DOPAC, serotonin, 5-HIAA, glycine, taurine, GABA, glutamate, PEA, and histamine. Methods: Neurological impact of the Masgutova Neurosensorimotor Reflex Method on the magnitude of changes in neurotransmitters was assessed by an external controlled and double-blind method using patients from one of the four diagnosis groups: 1) global developmental disorders;2) cerebral palsy, Traumatic Brain Injury (TBI), Acute Brain Injury (ABI), and seizures;3) ADD/ADHD;and 4) anxiety disorders. Results: The post-MNRI results in participants show a tendency for regulation of the above neurotransmitters resulting in their calming down, decrease of hypervigilance, stress resilience increase, improvements in behavioral and emotional regulation, positive emotions, and cognitive processes control. Conclusion: The application of the Masgutova Neurosensorimotor Reflex Method as a therapy modality offers a novelty paradigm for the treatment using neuro- and immune-modulation technologies presenting a non-pharmaceutical approach, based on use of neurosensorimotor reflex circuit concept.
文摘This research reports on potentiality in the solar chimney as an exhaust ventilation device through a hypothetical statement that convenient weather conditions exist on sub-humid warm climate to enhance the performance ofa SC （solar chimney）, in order to increase ventilation to dissipate metabolic heat from inhabitants of airtight buildings. The methodology used in this research integrates the use of simplified mathematical models that predict in a semi-empirical way a potential volume flow through estimation of kinetic power generation, by natural circulation and stack effect in a typical covered-plate air solar collector. The study was carried out during the warm month of April, an important warm season for Colima, where the prevailing wind is about 6.8 m/s, the average temperature of maximums and minimums oscillates between 15.4℃and 34.4 ℃ with average relative humidity of 65% according to national weather service. A scale model of the SC was installed on environmental chambers. The results showed that this device is capable of generating ventilation conditions around light breeze parameters near between 0.6 m/s and 1.5 m/s width, a better performance at day in 45% approximately than the night values, making ventilation rates up 0.24 ACH （air changes per hour） as average air change rate.
基金Fernanda Batistel(FB)was supported by Coordenacao de Aperfeicoamento de Pessoal de Nível Superior(CAPES)Juan Loor(JL)was supported by National Institute of Food and Agriculture(Grant:ILLU-538-914)Zinpro Corporation provided support to Juan J.Loor and Michael T.Socha
文摘Background: Immune dysfunction and a higher risk of uterine infections are characteristics of the transition into lactation in dairy cows. The supply of complexed trace minerals, which are more bioavailable, could help overcome the greater needs of these nutrients in tissues around parturition and early lactation.Results: Twenty Holstein cows received an oral bolus with a mix of inorganic trace minerals(INO) or complexed trace minerals(AAC) to achieve 75, 65, 11, and 1 ppm supplemental Zn, Mn, Cu, and Co, respectively, in the total diet dry matter from -30 d through +30 d relative to parturition. Blood for polymorphonuclear leukocyte(PMNL) isolation was collected at-30,-15, +10, and + 30 d relative to parturition, whereas endometrium biopsies were performed at +14 and +30 d. Feeding AAC led to greater PMNL expression of genes related with inflammation response(DDX58), oxidative stress response(MPO), eicosanoid metabolism(PLA2G4A and ALOX5AP), transcription regulation(PPARG), and cellular adhesion(TLN1). The upregulation by AAC in endometrium of genes related with inflammation response( TLR2, TLR4, NFKB1, TNF, IL6, IL1 B, IL10, IL8), prostaglandin synthesis(PTGS2, PTGES), and antioxidant responses(NFE2 L2, SOD1) indicated a faster remodeling of uterine tissue and potentially greater capacity to control a local bacterial invasion.Conclusions: Data indicate that trace mineral supplementation from amino acid complexes improves PMNL activity and allows the prompt recovery of uterine tissue during early lactation. As such, the benefits of complexed trace minerals extend beyond an improvement of liver function and productive performance.
基金supported by the Department of Defense (DoD)through the Breast Cancer Research Program (BCRP)under Award Nos.W81XWH-21-1-0062 and W81XWH-21-1-0063.
文摘Objective.To develop a 3D shear wave elastography(SWE)technique using a 2D row column addressing(RCA)array,with either external vibration or acoustic radiation force(ARF)as the shear wave source.Impact Statement.The proposed method paves the way for clinical translation of 3D SWE based on the 2D RCA,providing a low-cost and high volume rate solution that is compatible with existing clinical systems.Introduction.SWE is an established ultrasound imaging modality that provides a direct and quantitative assessment of tissue stiffness,which is significant for a wide range of clinical applications including cancer and liver fibrosis.SWE requires high frame rate imaging for robust shear wave tracking.Due to the technical challenges associated with high volume rate imaging in 3D,current SWE techniques are typically confined to 2D.Advancing SWE from 2D to 3D is significant because of the heterogeneous nature of tissue,which demands 3D imaging for accurate and comprehensive evaluation.Methods.A 3D SWE method using a RCA array was developed with a volume rate up to 2000 Hz.The performance of the proposed method was systematically evaluated on tissue-mimicking elasticity phantoms and in an in vivo case study.Results.3D shear wave motion induced by either external vibration or ARF was successfully detected with the proposed method.Robust 3D shear wave speed maps were reconstructed for phantoms and in vivo.Conclusion.The high volume rate 3D imaging provided by the 2D RCA array provides a robust and practical solution for 3D SWE with a clear pathway for future clinical translation.
基金National Institutes of Health grant#R01 NS076491supported by American Heart Association grant 23PRE1012781.
文摘Flow diverting stents are braided,metallic endoluminal devices widely used to treat intracranial aneurysms.Bioresorbable flow diverters(BRFDs)are gaining traction as the next generation of flow diverter technology.BRFDs aim to occlude and heal the aneurysm before safely dissolving into the body,mitigating or eliminating complications associated with the permanent presence of conventional flow diverters such as thromboembolism and stenosis.Additional putative advantages of a BRFD include a reduction in metal induced medical imaging artifacts,a restoration of physiological vasoreactivity,and allowing physicians to re-access the aneurysm if an additional procedure is required.In this current study,iron-manganese-nitrogen(FeMnN)alloy BRFDs and permanent control FDs composed of an industry standard Cobalt-Nickel-Chromium alloy were deployed in the rabbit aorta.MicroCT and SEM corrosion analysis determined the FeMnN wire volumes and cross-sectional areas had reduced approximately 85%and 95%after 3-and 6-months implantation duration,respectively.Histo-logical analysis demonstrated the BRFDs exhibited suitable biocompatibility,with no cases of in-stent throm-bosis,clinically significant stenosis,or adverse tissue responses observed.Immunohistochemistry revealed the neointimas surrounding the BRFDs featured a confluent endothelium covering several layers of smooth muscle cells,with macrophages adjacent to the device wires.The macrophages were able to penetrate the corrosion product and were observed transporting corrosion products away from the implant site.This current work provides primary in vivo corrosion and biocompatibility data to the field for FeMn alloys,which we feel will stimulate and inform the design of next-generation bioresorbable endovascular devices.
基金supported by the Natural Science Foundation of Shaanxi Province(Key Program),No.2021JZ-60(to HZ)。
文摘The unfolded protein response is a cellular pathway activated to maintain proteostasis and prevent cell death when the endoplasmic reticulum is overwhelmed by unfolded proteins.However,if the unfolded protein response fails to restore endoplasmic reticulum homeostasis,it can trigger proinflammatory and pro-death signals,which are implicated in various malignancies and are currently being investigated for their role in retinal degenerative diseases.This paper reviews the role of the unfolded protein responsein addressing endoplasmic reticulumstress in retinal degenerative diseases.The accumulation of ubiquitylated misfolded proteins can lead to rapid destabilization of the proteome and cellular demise.Targeting endoplasmic reticulum stress to alleviate retinal pathologies involves multiple strategies,including the use of chemical chaperones such as 4-phenylbutyric acid and tauroursodeoxycholic acid,which enhance protein folding and reduce endoplasmic reticulum stress.Small molecule modulators that influence endoplasmic reticulum stress sensors,including those that increase the expression of the endoplasmic reticulum stress regulator X-box binding protein 1,are also potential therapeutic agents.Additionally,inhibitors of the RNAse activity of inositol-requiring transmembrane kinase/endoribonuclease 1,a key endoplasmic reticulum stress sensor,represent another class of drugs that could prevent the formation of toxic aggregates.The activation of nuclear receptors,such as PPAR and FXR,may also help mitigate ER stress.Furthermore,enhancing proteolysis through the induction of autophagy or the inhibition of deubiquitinating enzymes can assist in clearing misfolded proteins.Combination treatments that involve endoplasmicreticulum-stress-targeting drugs and gene therapies are also being explored.Despite these potential therapeutic strategies,significant challenges remain in targeting endoplasmic reticulum stress for the treatment of retinal degeneration,and further research is essential to elucidate the mechanisms underlying human retinal diseases and to develop effective,well-tolerated drugs.The use of existing drugs that target inositol-requiring transmembrane kinase/endoribonuclease 1 and X-box binding protein 1 has been associated with adverse side effects,which have hindered their clinical translation.Moreover,signaling pathways downstream of endoplasmic reticulum stress sensors can contribute to therapy resistance.Addressing these limitations is crucial for developing drugs that can be effectively used in treating retinal dystrophies.In conclusion,while the unfolded protein response is a promising therapeutic target in retinal degenerative diseases,additional research and development efforts are imperative to overcome the current limitations and improve patient outcomes.
基金Legislative-Citizen Commission on Minnesota Resources(M.L.2010,Chp.362,Sec.2,Subd.6c‘Healthy Forests to Resist Invasion’,to P.B.R.)Integrative Graduate Education and Research Traineeship:Risk Analysis for Introduced Species and Genotypes(NSF DGE-0653827)+2 种基金University of Minnesota Graduate School Fellowshipthe Hubachek Wilderness Research Foundationthe Dayton Fund of the Bell Museum of Natural History.
文摘Aims Theory predicts that the success of introduced species is related to the diversity of native species through trait-based processes.Abiotic site characteristics may also affect a site’s susceptibility to invasion.We quantified resident plant species richness,phylogenetic diversity and several abiotic site characteristics for 24 oak forests in Minnesota,USA,to assess their impact on the abundance of a widespread,introduced terrestrial plant species,common buckthorn(Rhamnus cathartica L.).Specifically,we asked(1)whether resident species richness and phylogenetic diversity affected the abundance of R.cathartica and(2)what site characteristics explained the overall abundance of R.cathartica.Methods Our survey included 24 oak-dominated stands in Minnesota’s deciduous forests.In each stand,we identified all species in 16 plots.We also measured a series of environmental site characteristics,including canopy openness(a proxy for light availability),percent bare soil,soil pH,percent sand,an index of propagule availability,duff layer thickness(a proxy for earthworm activity),an index of insolation and slope.For all species present in at least one site,we estimated a community phylogeny.We combined all sitelevel characteristics,including phylogenetic diversity of the resident plant species,in a multiple regression model to examine site-level drivers of community invasibility.Important Findings Results indicate that sites with higher overall plant phylogenetic diversity harbor less R.cathartica,even though native species richness was not significantly related to R.cathartica abundance.Regression analyses indicated that,in addition to resident species phylogenetic diversity,the most important predictors of R.cathartica abundance were canopy openness and the amount of bare soil,both positively related to the abundance of the invader.By combining the effects of abiotic site characteristics and resident species phylogenetic diversity in a model that predicted the abundance of R.cathartica,we were able to simultaneously account for a wide range of factors that might influence invasibility.Overall,our results suggest that management strategies aimed at reducing disturbances that lead to increased bare soil and light levels may be more successful if they also maximize phylogenetic diversity of the resident plant community.
基金support of Carlo Wolf(School of Mechanical Engineering,University of Applied Sciences Stralsund)for support with the CT analysis and metallographic preparation,Aubrey L.Ehle(Indiana University School of Medicine)for support with the radiopacity assessment,and Amani Gillette(Morgridge Institute for Research)for support with the cytotoxicity analysis.Parts of this study were completed using Michigan Technological University’s Applied Chemical and Morphological Analysis Laboratory.RG and NP were partially supported by NIH R15HL167221AO is supported by American Heart Association grant 23PRE1012781.
文摘Flow diverter devices are small stents used to divert blood flow away from aneurysms in the brain,stagnating flow and inducing intra-aneurysmal thrombosis which in time will prevent aneurysm rupture.Current devices are formed from thin(~25μm)wires which will remain in place long after the aneurysm has been mitigated.As their continued presence could lead to secondary complications,an absorbable flow diverter which dissolves into the body after aneurysm occlusion is desirable.The absorbable metals investigated to date struggle to achieve the necessary combination of strength,elasticity,corrosion rate,fragmentation resistance,radiopacity,and biocompatibility.This work proposes and investigates a new composite wire concept combining absorbable iron alloy(FeMnN)shells with one or more pure molybdenum(Mo)cores.Various wire configurations are produced and drawn to 25–250μm wires.Tensile testing revealed high and tunable mechanical properties on par with existing flow diverter materials.In vitro degradation testing of 100μm wire in DMEM to 7 days indicated progressive corrosion and cracking of the FeMnN shell but not of the Mo,confirming the cathodic protection of the Mo by the FeMnN and thus mitigation of premature fragmentation risk.In vivo implantation and subsequentμCT of the same wires in mouse aortas to 6 months showed meaningful corrosion had begun in the FeMnN shell but not yet in the Mo filament cores.In total,these results indicate that these composites may offer an ideal combination of properties for absorbable flow diverters.
文摘A small but measurable percentage of major hepatic resection patients will experience major morbidity or mortality due post-hepatectomy liver failure after major liver resection.Pre-operative optimization,in particular pre-operative induction of liver growth to increase the future liver remnant(FLR)volume,has become frequently used in patients considered to be high risk.However,this process of hepatic augmentation remains incompletely understood,particularly in humans.Recent advances in techniques for FLR management have resulted in dramatic expansion of the liver surgeon’s armamentarium.
文摘Some authors have studied a series of (Pd0.9965 Fe0.0035)1-xMnx alloys which exhibit distinctly different magnetic properties depending upon the Mn concentration x. It is attributed to the competing interaction in magnetic systems, In general, for a large number of alloy systems with mixed ferro-antiferromagnetic interactions, the competition leads to a diversity of magnetic struetures and critical phenomena.
文摘The First UMN-CAS Bilateral Seminar was successfully held in Xi＇an, China, May 27-28,2014. A summary of the 1 st Bilateral Seminar was published in Particuology 16 （2014）, 227-229. The Second UMN-CAS Bilateral Seminar was held at 3M Innovation Center, St. Paul, Minnesota, October 7-8, 2015.
文摘Photon-counting computed tomography(PCCT)represents a significant advancement in pediatric cardiovascular imaging.Traditional CT systems employ energy-integrating detectors that convert X-ray photons into visible light,whereas PCCT utilizes photon-counting detectors that directly transform X-ray photons into electric signals.This direct conversion allows photon-counting detectors to sort photons into discrete energy levels,thereby enhancing image quality through superior noise reduction,improved spatial and contrast resolution,and reduced artifacts.In pediatric applications,PCCT offers substantial benefits,including lower radiation doses,which may help reduce the risk of malignancy in pediatric patients,with perhaps greater potential to benefit those with repeated exposure from a young age.Enhanced spatial resolution facilitates better visualization of small structures,vital for diagnosing congenital heart defects.Additionally,PCCT’s spectral capabilities improve tissue characterization and enable the creation of virtual monoenergetic images,which enhance soft-tissue contrast and potentially reduce contrast media doses.Initial clinical results indicate that PCCT provides superior image quality and diagnostic accuracy compared to conven-tional CT,particularly in challenging pediatric cardiovascular cases.As PCCT technology matures,further research and standardized protocols will be essential to fully integrate it into pediatric imaging practices,ensuring optimized diagnostic outcomes and patient safety.
基金the financial support from FORMAS(2018-01029)the Swedish Institute(01132-2022)for supporting Ivan Motsnyi’s visit and research at Swedish University of Agricultural Sciences。
文摘Emerging new races of wheat stem rust(Puccinia graminis f.sp.tritici)are threatening global wheat(Triticum aestivum L.)production.Host resistance is the most effective and environmentally friendly method of controlling stem rust.The stem rust resistance gene Sr59 was previously identified within a T2DS 2RL wheat-rye whole arm translocation,providing broad-spectrum resistance to various stem rust races.Seedling evaluation,molecular marker analysis,and cytogenetic studies identified wheat-rye introgression line#284 containing a new translocation chromosome T2BL 2BS-2RL.This line has demonstrated broad-spectrum resistance to stem rust at the seedling stage.Seedling evaluation and cytogenetic analysis of three backcross populations between the line#284 and the adapted cultivars SLU-Elite,Navruz,and Linkert confirmed that Sr59 is located within the short distal 2RL translocation.This study aimed physical mapping of Sr59 in the 2RL introgression segment and develop a robust molecular marker for marker-assisted selection.Using genotyping-by-sequencing(GBS),GBS-derived SNPs were aligned with full-length annotated rye nucleotide-binding leucine-rich repeat(NLR)genes in the parental lines CS ph1b,SLU238,SLU-Elite,Navruz,and Linkert,as well as in 33 BC4F5progeny.Four NLR genes were identified on the 2R chromosome,with Chr2R_NLR_60 being tightly linked to the Sr59resistance gene.In-silico functional enrichment analysis of the translocated 2RL region(25,681,915 bp)identified 223 genes,with seven candidate genes associated with plant disease resistance and three linked to agronomic performance,contributing to oxidative stress response,protein kinase activity,and cellular homeostasis.These findings facilitate a better understanding of the genetic basis of stem rust resistance provided by Sr59.
基金supported by the National Natural Science Foundation of China(32300157)the Shanghai Municipal Science and Technology Commission(19411964900)+1 种基金the Major Research and Development Project of Innovative Drugs,Ministry of Science and Technology of China(2017ZX09304005)the Wellcome Trust.
文摘Antimicrobial resistance is a global public health threat,and the World Health Organization(WHO)has announced a priority list of the most threatening pathogens against which novel antibiotics need to be developed.The discovery and introduction of novel antibiotics are time-consuming and expensive.According to WHO’s report of antibacterial agents in clinical development,only 18 novel antibiotics have been approved since 2014.Therefore,novel antibiotics are critically needed.Artificial intelligence(AI)has been rapidly applied to drug development since its recent technical breakthrough and has dramatically improved the efficiency of the discovery of novel antibiotics.Here,we first summarized recently marketed novel antibiotics,and antibiotic candidates in clinical development.In addition,we systematically reviewed the involvement of AI in antibacterial drug development and utilization,including small molecules,antimicrobial peptides,phage therapy,essential oils,as well as resistance mechanism prediction,and antibiotic stewardship.
文摘BACKGROUND The Warburg effect is common in cancers.Lactate and its receptor GPR81 play an important role in cancer progression.It is widely accepted that membrane receptor nuclear translocation plays some novel role in cancer pathology.The mechanism by which the lactate/GPR81 axis regulates cancer malignancy remains unclear.AIM To elucidate the mechanism of GPR81 nuclear transportation promoted by exogenous lactate.METHODS Lung cancer cells were stimulated with exogenous lactate and GPR81 levels were measured by immunofluoresence and western blot analysis in membrane,cytoplasmic,and nuclear fractions.Lung cancer cells were transduced with a mutant GPR81 nuclear localization signal(NLS)construct,wild type GPR81 or empty vector and used to examine how GPR81 nuclear transportation affects lung cancer cells malignancy in vitro and in vivo.Immunoprecipitation Proteomics analysis and Chromatin immunoprecipitation(ChIP)sequencing were used to determine GPR81 interacting proteins and genes.RESULTS In response to hypoxia/Lactate stimulation,GPR81 translocates and accumulates in the nucleus of lung cancer cells.Functionally,GPR81 nuclear translocation promotes cancer cell proliferation and motility.Depletion of the GPR81 NLS depletes GPR81 nuclear levels and decreases cancer cell growth and invasion in vitro,as well as cancer cell malignancy in vivo.Proteomics analysis revealed a set of proteins including SFPQ,that interact with GPR81 in the cancer cell nucleus.Notably,the interaction of GPR81 with SFPQ promotes cancer cell growth and motility.ChIP sequencing analysis discovered that there is a set of genes targeted by GPR81.CONCLUSION The interaction of GPR81 with SFPQ promotes cancer cell malignancy.GPR81 nuclear translocation is critical in conferring cancer progression and may be a potential therapeutic target for limiting cancer progression.
文摘There is a growing body of clinical research on the utility of synthetic data derivatives,an emerging research tool in medicine.In nephrology,clinicians can use machine learning and artificial intelligence as powerful aids in their clinical decision-making while also preserving patient privacy.This is especially important given the epidemiology of chronic kidney disease,renal oncology,and hypertension worldwide.However,there remains a need to create a framework for guidance regarding how to better utilize synthetic data as a practical application in this research.
