通用营销公司(Marketing General Incorporated,简称“MGI”)近期发布了《2025协会展望报告》(2025Association Outlook Report Summary)。自2019年以来,MGI持续收集协会高管对未来一年的计划和预期的洞察,这些数据揭示了对2025年的乐...通用营销公司(Marketing General Incorporated,简称“MGI”)近期发布了《2025协会展望报告》(2025Association Outlook Report Summary)。自2019年以来,MGI持续收集协会高管对未来一年的计划和预期的洞察,这些数据揭示了对2025年的乐观情绪,协会领导预计会员数量和参与度将增加,但也预计来年将面临一些挑战。展开更多
The monkeypox virus(MPXV)has triggered a current outbreak globally.Genome sequencing of MPXV and rapid tracing of genetic variants will benefit disease diagnosis and control.It is a significant challenge but necessary...The monkeypox virus(MPXV)has triggered a current outbreak globally.Genome sequencing of MPXV and rapid tracing of genetic variants will benefit disease diagnosis and control.It is a significant challenge but necessary to optimize the strategy and application of rapid full-length genome identification and to track variations of MPXV in clinical specimens with low viral loads,as it is one of the DNA viruses with the largest genome and the most AT-biased,and has a significant number of tandem repeats.Here we evaluated the performance of metagenomic and amplicon sequencing techniques,and three sequencing platforms in MPXV genome sequencing based on multiple clinical specimens of five mpox cases in Chinese mainland.We rapidly identified the full-length genome of MPXV with the assembly of accurate tandem repeats in multiple clinical specimens.Amplicon sequencing enables cost-effective and rapid sequencing of clinical specimens to obtain high-quality MPXV genomes.Third-generation sequencing facilitates the assembly of the terminal tandem repeat regions in the monkeypox virus genome and corrects a common misassembly in published sequences.Besides,several intra-host single nucleotide variations were identified in the first imported mpox case.This study offers an evaluation of various strategies aimed at identifying the complete genome of MPXV in clinical specimens.The findings of this study will significantly enhance the surveillance of MPXV.展开更多
Blood doping remains a significant problem that threatensthe integrity ofsport.The currentindirect method for detecting blood doping involves the longitudinal measurement of an athlete’s haematological variables and i...Blood doping remains a significant problem that threatensthe integrity ofsport.The currentindirect method for detecting blood doping involves the longitudinal measurement of an athlete’s haematological variables and identification of suspicious results that are indicative of doping(i.e.,the athlete biological passport).While this has played a significant role in the protection of clean sport,improvements are needed.The development of a transcriptomic test,that can be used to detect the use of blood doping has been discussed and researched for decades and yet,an anti-doping test that can be adopted by the World Anti-Doping Agency(WADA)is yet to be established.With recent advancements in our understanding,as well as in methods of sample collection,the possibility of a transcriptomic test that can be used by WADA,is imminent.There are,however,several practical considerations that mustfirst be made,that will be highlighted in this perspective article.展开更多
国家基因库生命大数据平台(China National GeneBank DataBase,CNGBdb)是一个致力于生命科学多组学数据归档和开放共享的数据库平台,是深圳国家基因库的核心功能“三库两平台”中生物信息数据库的对外服务平台,拥有深圳国家基因库丰富...国家基因库生命大数据平台(China National GeneBank DataBase,CNGBdb)是一个致力于生命科学多组学数据归档和开放共享的数据库平台,是深圳国家基因库的核心功能“三库两平台”中生物信息数据库的对外服务平台,拥有深圳国家基因库丰富的样本资源、数据资源、合作项目资源和强大的数据计算和分析能力等优势。生命科学研究已经进入到了一个以高通量多组学数据为基础的大数据时代,迫切需要加强国际合作和信息共享。随着中国经济的发展和在生命科学研究领域的研究项目投入力度的加大,需要建立相关的生命大数据归档和共享的平台,来促进我国生命科学研究项目中生成的基因组学数据的系统管理、开放共享与合理利用。目前,CNGBdb主要提供生命科学研究相关的数据归档、知识搜索、数据管理、数据计算和数据服务等服务。其归档和共享的数据类型,主要包括项目、样本、实验、测序、组装、变异、序列等。截止2020年5月22号,CNGBdb已接受了全球生命科学科研工作者提交的研究项目达2176个,归档的基因组学数据量超过2221 TB。未来,CNGBdb将继续推动生命科学研究多组学数据的开放共享和产业应用,完善基因组学数据的归档和共享功能,提升其服务生命科学数据开放共享的能力。CNGBdb的网址是:https://db.cngb.org/。展开更多
The genetically engineered pig is regarded as an optimal source of organ transplantation for humans and an excellent model for human disease research,given its comparable physiology to human beings.A myriad of single-...The genetically engineered pig is regarded as an optimal source of organ transplantation for humans and an excellent model for human disease research,given its comparable physiology to human beings.A myriad of single-cell RNA sequencing(sc RNA-seq)data on humans has been reported,but such data on pigs are scarce.Here,we apply sc RNA-seq technology to study the cellular heterogeneity of 3-month-old pig lungs,generating the single-cell atlas of 13,580 cells covering 16 major cell types.Based on these data,we systematically characterize the similarities and differences in the cellular cross-talk and expression patterns of respiratory virus receptors in each cell type of pig lungs compared with human lungs.Furthermore,we analyze pig lung xenotransplantation barriers and reported the cell-type expression patterns of 10 genes associated with pig-to-human immunobiological incompatibility and coagulation dysregulation.We also investigate the conserved transcription factors(TFs)and their candidate target genes and constructed five conserved TF regulatory networks in the main cell types shared by pig and human lungs.Finally,we present a comprehensive and openly accessible online platform,Scdb Lung.Our sc RNA-seq atlas of the domestic pig lung and Scdb Lung database can guide pig lung research and clinical applicability.展开更多
Parkinson's disease(PD)is a neurodegenerative disease,leading to the impairment of movement execution.PD pathogenesis has been largely investigated,either limited to bulk transcriptomic levels or at certain cell t...Parkinson's disease(PD)is a neurodegenerative disease,leading to the impairment of movement execution.PD pathogenesis has been largely investigated,either limited to bulk transcriptomic levels or at certain cell types,which failed to capture the cellular heterogeneity and intrinsic interplays among distinct cell types.Here,we report the application of single-nucleus RNA-seq on midbrain,striatum,and cerebellum of theα-syn-A53 T mouse,a well-established PD mouse model,and matched controls,generating the first single cell transcriptomic atlas for the PD model mouse brain composed of 46,174 individual cells.Additionally,we comprehensively depicte the dysfunctions in PD pathology,covering the elevation of NF-k B activity,the alteration of ion channel components,the perturbation of protein homeostasis network,and the dysregulation of glutamatergic signaling.Notably,we identify a variety of cell types closely associated with PD risk genes.Taken together,our study provides valuable resources to systematically dissect the molecular mechanism of PD pathogenesis at the single-cell resolution,which facilitates the development of novel approaches for diagnosis and therapies against PD.展开更多
We report electronic and magnetic properties of full Heusler Pd_(2)TiIn based on first principles calculations.This compound has been variously characterized as magnetic or non-magnetic.We use first principles calcula...We report electronic and magnetic properties of full Heusler Pd_(2)TiIn based on first principles calculations.This compound has been variously characterized as magnetic or non-magnetic.We use first principles calculations with accurate methods to reexamine this issue.We find that ideal ordered Heusler Pd_(2)TiIn remains non-magnetic,in accord with prior work.However,we do find that it is possible to explain the magnetism seen in experiments through disorder and in particular we find that site disorder can lead to moment formation in this compound.In addition,we find an alternative low energy cubic crystal structure,which will be of interest to explore experimentally.展开更多
Fulminant myocarditis(FM)is a severe inflammatory condition of the myocardium that often results in sudden death,particularly in young individuals.In this study,we employed single-nucleus and spatial transcriptomics t...Fulminant myocarditis(FM)is a severe inflammatory condition of the myocardium that often results in sudden death,particularly in young individuals.In this study,we employed single-nucleus and spatial transcriptomics to perform a comprehensive analysis of coxsackievirus B3(CVB3)-induced FM in A/J mice,spanning seven distinct time points pre-and post-treatment.Our findings reveal that mesothelial cells play a critical role in the early stage of myocarditis by acting as primary targets for CVB3 infection.This triggers the activation of macrophages,initiating a cascade of inflammation.Subsequently,pro-inflammatory Inflammatory_Mac and T cells infiltrate the myocardium,driving tissue damage.We also identified Cd8+effector T cells as key mediators of cardiomyocyte injury.These cells release cytotoxic molecules,particularly IFN-γ,which modulates the expression of Spi1,a factor implicated in exacerbating cardiomyocyte death and amplifying disease progression.Therapeutic interventions targeting the IFN-γ/Spi1 axis demonstrated significant efficacy in FM models.Notably,intravenous immunoglobulin(IVIG)treatment reduced mortality,suppressed viral proliferation,and mitigated the hyperinflammatory state of FM.IVIG therapy also downregulated IFN-γ and Spi1 expression,underscoring its immunomodulatory and therapeutic potential.This comprehensive spatiotemporal transcriptomic analysis provides profound insights into the pathogenesis of FM and highlights actionable therapeutic targets,paving the way for more effective management strategies for this life-threatening condition.展开更多
While metal materials historically have served as permanent implants and were designed to avoid degradation,next generation bioabsorbable metals for medical devices such as vascular stents are under development,which ...While metal materials historically have served as permanent implants and were designed to avoid degradation,next generation bioabsorbable metals for medical devices such as vascular stents are under development,which would elute metal ions and corrosion byproducts into tissues.The fate of these eluted products and their local distribution in vascular tissue largely under studied.In this study,we employ a high spatial resolution spectrometric imaging modality,laser ablation inductively coupled plasma time-of-flight mass spectrometry(LA-ICP-TOF-MS)to map the metal distribution,(herein refered to as laser ablation mapping,or LAM)from Mg alloys within the mouse vascular system and approximate their local concentrations.We used a novel rare earth element bearing Mg alloy(WE22)wire implanted within the abdominal aorta of transgenic hypercholesterolemic mice(APOE/)to simulate a bioabsorbable vascular prosthesis for up to 30 days.We describe qualitatively and semi-quantitatively implant-derived corrosion product presence throughout the tissue cross sections,and their approximate concentrations within the various vessel structures.Additionally,we report the spatial changes of corrosion products,which we postulate are mediated by phagocytic inflammatory cells such as macrophages(MΦ’s).展开更多
Chikungunya virus(CHIKV)infection,responsible for chikungunya fever and occasionally severe symptoms,has emerged as an increasing global health concern following several large-scale outbreaks from Africa,Asia,Europe,a...Chikungunya virus(CHIKV)infection,responsible for chikungunya fever and occasionally severe symptoms,has emerged as an increasing global health concern following several large-scale outbreaks from Africa,Asia,Europe,and America.Over the past two decades,South and Southeast Asia regions have gradually become hot spots for outbreaks involving multiple CHIKV lineages.In China,most CHIKV infections are imported,making it crucial to trace the origins and transmission routes for effective prevention and control.In January 2024,a case of imported chikungunya fever was confirmed in Guangzhou City,Guangdong Province,China.However,the serum CHIKV viral concentration was too low for cultivation[reverse transcription-polymerase chain reaction(RT-PCR)detection,cycle threshold=32.62].Despite this,we suc-cessfully obtained the viral genome sequence directly from the whole blood sample using an optimized meta-transcriptomic sequencing strategy,achieving a full-length viral genome with an average depth of 54.3x.Further analysis confirmed that the CHIKV virus belonged to the Asian lineage,traced to Timor-Leste,where an endemic CHIKV outbreak had been reported in January 2024,consistent with the patient's travel history.Finally,we analyzed genetic evolutionary trends and amino acid site variations.This study highlights the iden-tification of a CHIKV infection origin using direct whole-blood metatranscriptomic sequencing,a valuable method for rapidly sequencing low viral-load samples.展开更多
The brain of the domestic pig(Sus scrofa domesticus)has drawn considerable attention due to its high similarities to that of humans.However,the cellular compositions of the pig brain(PB)remain elusive.Here we investig...The brain of the domestic pig(Sus scrofa domesticus)has drawn considerable attention due to its high similarities to that of humans.However,the cellular compositions of the pig brain(PB)remain elusive.Here we investigated the single-nucleus transcriptomic profiles of five regions of the PB(frontal lobe,parietal lobe,temporal lobe,occipital lobe,and hypothalamus)and identified 21 cell subpopulations.The cross-species comparison of mouse and pig hypothalamus revealed the shared and specific gene expression patterns at the single-cell resolution.Furthermore,we identified cell types and molecular pathways closely associated with neurological disorders,bridging the gap between gene mutations and pathogenesis.We reported,to our knowledge,the first single-cell atlas of domestic pig cerebral cortex and hypothalamus combined with a comprehensive analysis across species,providing extensive resources for future research regarding neural science,evolutionary developmental biology,and regenerative medicine.展开更多
The importance of structural variants(SVs)for human phenotypes and diseases is now recognized.Although a variety of SV detection platforms and strategies that vary in sensitivity and specificity have been developed,fe...The importance of structural variants(SVs)for human phenotypes and diseases is now recognized.Although a variety of SV detection platforms and strategies that vary in sensitivity and specificity have been developed,few benchmarking procedures are available to confidently assess their performances in biological and clinical research.To facilitate the validation and application of these SV detection approaches,we established an Asian reference material by characterizing the genome of an Epstein-Barr virus(EBV)-immortalized B lymphocyte line along with identified benchmark regions and high-confidence SV calls.We established a high-confidence SV callset with 8938 SVs by integrating four alignment-based SV callers,including 109×Pacific Bio sciences(PacBio)continuous long reads(CLRs),22×PacBio circular consensus sequencing(CCS)reads,104×Oxford Nanopore Technologies(ONT)long reads,and 114×Bionano optical mapping platform,and one de novo assembly-based SV caller using CCS reads.A total of 544 randomly selected SVs were validated by PCR amplification and Sanger sequencing,demonstrating the robustness of our SV calls.Combining trio-binning-based haplotype assemblies,we established an SV benchmark for identifying false negatives and false positives by constructing the continuous high-confidence regions(CHCRs),which covered 1.46 gigabase pairs(Gb)and 6882 SVs supported by at least one diploid haplotype assembly.Establishing high-confidence SV calls for a benchmark sample that has been characterized by multiple technologies provides a valuable resource for investigating SVs in human biology,disease,and clinical research.展开更多
We have recently pointed out how the existence of massive neutrinos would imply the possibility of forming very massive self-gravitating systems of neutrinos held in equilibrium by their Fermi pressure. In dealing wit...We have recently pointed out how the existence of massive neutrinos would imply the possibility of forming very massive self-gravitating systems of neutrinos held in equilibrium by their Fermi pressure. In dealing with this question, the neutrinos have been assumed to be in a degenerate state and their Fermi temperature has been computed for a selected number of equilibrium configurations. All the展开更多
文摘通用营销公司(Marketing General Incorporated,简称“MGI”)近期发布了《2025协会展望报告》(2025Association Outlook Report Summary)。自2019年以来,MGI持续收集协会高管对未来一年的计划和预期的洞察,这些数据揭示了对2025年的乐观情绪,协会领导预计会员数量和参与度将增加,但也预计来年将面临一些挑战。
基金supported by the National Key Research and Development Program of China(2022YFC2303401,2022YFC2304100,2016YFD0500301,2021YFC0863300)the Beijing Science and Technology Plan(Z211100002521017)the National Natural Science Foundation of China(82241080)。
文摘The monkeypox virus(MPXV)has triggered a current outbreak globally.Genome sequencing of MPXV and rapid tracing of genetic variants will benefit disease diagnosis and control.It is a significant challenge but necessary to optimize the strategy and application of rapid full-length genome identification and to track variations of MPXV in clinical specimens with low viral loads,as it is one of the DNA viruses with the largest genome and the most AT-biased,and has a significant number of tandem repeats.Here we evaluated the performance of metagenomic and amplicon sequencing techniques,and three sequencing platforms in MPXV genome sequencing based on multiple clinical specimens of five mpox cases in Chinese mainland.We rapidly identified the full-length genome of MPXV with the assembly of accurate tandem repeats in multiple clinical specimens.Amplicon sequencing enables cost-effective and rapid sequencing of clinical specimens to obtain high-quality MPXV genomes.Third-generation sequencing facilitates the assembly of the terminal tandem repeat regions in the monkeypox virus genome and corrects a common misassembly in published sequences.Besides,several intra-host single nucleotide variations were identified in the first imported mpox case.This study offers an evaluation of various strategies aimed at identifying the complete genome of MPXV in clinical specimens.The findings of this study will significantly enhance the surveillance of MPXV.
文摘Blood doping remains a significant problem that threatensthe integrity ofsport.The currentindirect method for detecting blood doping involves the longitudinal measurement of an athlete’s haematological variables and identification of suspicious results that are indicative of doping(i.e.,the athlete biological passport).While this has played a significant role in the protection of clean sport,improvements are needed.The development of a transcriptomic test,that can be used to detect the use of blood doping has been discussed and researched for decades and yet,an anti-doping test that can be adopted by the World Anti-Doping Agency(WADA)is yet to be established.With recent advancements in our understanding,as well as in methods of sample collection,the possibility of a transcriptomic test that can be used by WADA,is imminent.There are,however,several practical considerations that mustfirst be made,that will be highlighted in this perspective article.
文摘国家基因库生命大数据平台(China National GeneBank DataBase,CNGBdb)是一个致力于生命科学多组学数据归档和开放共享的数据库平台,是深圳国家基因库的核心功能“三库两平台”中生物信息数据库的对外服务平台,拥有深圳国家基因库丰富的样本资源、数据资源、合作项目资源和强大的数据计算和分析能力等优势。生命科学研究已经进入到了一个以高通量多组学数据为基础的大数据时代,迫切需要加强国际合作和信息共享。随着中国经济的发展和在生命科学研究领域的研究项目投入力度的加大,需要建立相关的生命大数据归档和共享的平台,来促进我国生命科学研究项目中生成的基因组学数据的系统管理、开放共享与合理利用。目前,CNGBdb主要提供生命科学研究相关的数据归档、知识搜索、数据管理、数据计算和数据服务等服务。其归档和共享的数据类型,主要包括项目、样本、实验、测序、组装、变异、序列等。截止2020年5月22号,CNGBdb已接受了全球生命科学科研工作者提交的研究项目达2176个,归档的基因组学数据量超过2221 TB。未来,CNGBdb将继续推动生命科学研究多组学数据的开放共享和产业应用,完善基因组学数据的归档和共享功能,提升其服务生命科学数据开放共享的能力。CNGBdb的网址是:https://db.cngb.org/。
基金supported by China National Gene Bank(CNGB)financially supported by the National Natural Science Foundation of China(31670742)the Natural Science Foundation of Guangdong Province,China(2021A1515011109)。
文摘The genetically engineered pig is regarded as an optimal source of organ transplantation for humans and an excellent model for human disease research,given its comparable physiology to human beings.A myriad of single-cell RNA sequencing(sc RNA-seq)data on humans has been reported,but such data on pigs are scarce.Here,we apply sc RNA-seq technology to study the cellular heterogeneity of 3-month-old pig lungs,generating the single-cell atlas of 13,580 cells covering 16 major cell types.Based on these data,we systematically characterize the similarities and differences in the cellular cross-talk and expression patterns of respiratory virus receptors in each cell type of pig lungs compared with human lungs.Furthermore,we analyze pig lung xenotransplantation barriers and reported the cell-type expression patterns of 10 genes associated with pig-to-human immunobiological incompatibility and coagulation dysregulation.We also investigate the conserved transcription factors(TFs)and their candidate target genes and constructed five conserved TF regulatory networks in the main cell types shared by pig and human lungs.Finally,we present a comprehensive and openly accessible online platform,Scdb Lung.Our sc RNA-seq atlas of the domestic pig lung and Scdb Lung database can guide pig lung research and clinical applicability.
基金supported by the National Natural Science Foundation of China(31702074 and 31872309)Sanming Project of Medicine in Shenzhen(SZSM202011012)Science,Technology and Innovation Commission of Shenzhen Municipality(JCYJ20170412153100794)。
文摘Parkinson's disease(PD)is a neurodegenerative disease,leading to the impairment of movement execution.PD pathogenesis has been largely investigated,either limited to bulk transcriptomic levels or at certain cell types,which failed to capture the cellular heterogeneity and intrinsic interplays among distinct cell types.Here,we report the application of single-nucleus RNA-seq on midbrain,striatum,and cerebellum of theα-syn-A53 T mouse,a well-established PD mouse model,and matched controls,generating the first single cell transcriptomic atlas for the PD model mouse brain composed of 46,174 individual cells.Additionally,we comprehensively depicte the dysfunctions in PD pathology,covering the elevation of NF-k B activity,the alteration of ion channel components,the perturbation of protein homeostasis network,and the dysregulation of glutamatergic signaling.Notably,we identify a variety of cell types closely associated with PD risk genes.Taken together,our study provides valuable resources to systematically dissect the molecular mechanism of PD pathogenesis at the single-cell resolution,which facilitates the development of novel approaches for diagnosis and therapies against PD.
基金Supported by the National Natural Science Foundation of China(Grant Nos.51861145315,51672171,12074241and 51911530124)the Department of Energy,Basic Energy Sciences(Award DE-SC0019114)for work at the University of Missouri+3 种基金the Shanghai Municipal Science and Technology Commission Program(Grant No.19010500500)the China Scholarship Council(CSC)the Independent Research Project of State Key Laboratory of Advanced Special Steel and Shanghai Key Laboratory of Advanced Ferrometallurgy at Shanghai Universitythe State Key Laboratory of Solidification Processing in NWPU(Grant No.SKLSP201703)。
文摘We report electronic and magnetic properties of full Heusler Pd_(2)TiIn based on first principles calculations.This compound has been variously characterized as magnetic or non-magnetic.We use first principles calculations with accurate methods to reexamine this issue.We find that ideal ordered Heusler Pd_(2)TiIn remains non-magnetic,in accord with prior work.However,we do find that it is possible to explain the magnetism seen in experiments through disorder and in particular we find that site disorder can lead to moment formation in this compound.In addition,we find an alternative low energy cubic crystal structure,which will be of interest to explore experimentally.
基金supported by the High-performance Computing Platform of YaZhou Bay Science and Technology City Advanced Computing Center(YZBSTCACC)the National Natural Science Foundation of China(Nos.82330010 and 82241034 to DWW).
文摘Fulminant myocarditis(FM)is a severe inflammatory condition of the myocardium that often results in sudden death,particularly in young individuals.In this study,we employed single-nucleus and spatial transcriptomics to perform a comprehensive analysis of coxsackievirus B3(CVB3)-induced FM in A/J mice,spanning seven distinct time points pre-and post-treatment.Our findings reveal that mesothelial cells play a critical role in the early stage of myocarditis by acting as primary targets for CVB3 infection.This triggers the activation of macrophages,initiating a cascade of inflammation.Subsequently,pro-inflammatory Inflammatory_Mac and T cells infiltrate the myocardium,driving tissue damage.We also identified Cd8+effector T cells as key mediators of cardiomyocyte injury.These cells release cytotoxic molecules,particularly IFN-γ,which modulates the expression of Spi1,a factor implicated in exacerbating cardiomyocyte death and amplifying disease progression.Therapeutic interventions targeting the IFN-γ/Spi1 axis demonstrated significant efficacy in FM models.Notably,intravenous immunoglobulin(IVIG)treatment reduced mortality,suppressed viral proliferation,and mitigated the hyperinflammatory state of FM.IVIG therapy also downregulated IFN-γ and Spi1 expression,underscoring its immunomodulatory and therapeutic potential.This comprehensive spatiotemporal transcriptomic analysis provides profound insights into the pathogenesis of FM and highlights actionable therapeutic targets,paving the way for more effective management strategies for this life-threatening condition.
基金under Grant P41 GM135018(as well as Grant S10OD026786)from the National Institute of General Medical Sciences of the National Institutes of Healthpartially supported by the National Heart Blood Lung Institute under the award numbers R15HL167221-01 and R15HL167221-02(RJG).
文摘While metal materials historically have served as permanent implants and were designed to avoid degradation,next generation bioabsorbable metals for medical devices such as vascular stents are under development,which would elute metal ions and corrosion byproducts into tissues.The fate of these eluted products and their local distribution in vascular tissue largely under studied.In this study,we employ a high spatial resolution spectrometric imaging modality,laser ablation inductively coupled plasma time-of-flight mass spectrometry(LA-ICP-TOF-MS)to map the metal distribution,(herein refered to as laser ablation mapping,or LAM)from Mg alloys within the mouse vascular system and approximate their local concentrations.We used a novel rare earth element bearing Mg alloy(WE22)wire implanted within the abdominal aorta of transgenic hypercholesterolemic mice(APOE/)to simulate a bioabsorbable vascular prosthesis for up to 30 days.We describe qualitatively and semi-quantitatively implant-derived corrosion product presence throughout the tissue cross sections,and their approximate concentrations within the various vessel structures.Additionally,we report the spatial changes of corrosion products,which we postulate are mediated by phagocytic inflammatory cells such as macrophages(MΦ’s).
基金approved by the institutional review board of the Guangzhou Eighth People's Hospital(No.202312249)the patient provided written informed consent。
文摘Chikungunya virus(CHIKV)infection,responsible for chikungunya fever and occasionally severe symptoms,has emerged as an increasing global health concern following several large-scale outbreaks from Africa,Asia,Europe,and America.Over the past two decades,South and Southeast Asia regions have gradually become hot spots for outbreaks involving multiple CHIKV lineages.In China,most CHIKV infections are imported,making it crucial to trace the origins and transmission routes for effective prevention and control.In January 2024,a case of imported chikungunya fever was confirmed in Guangzhou City,Guangdong Province,China.However,the serum CHIKV viral concentration was too low for cultivation[reverse transcription-polymerase chain reaction(RT-PCR)detection,cycle threshold=32.62].Despite this,we suc-cessfully obtained the viral genome sequence directly from the whole blood sample using an optimized meta-transcriptomic sequencing strategy,achieving a full-length viral genome with an average depth of 54.3x.Further analysis confirmed that the CHIKV virus belonged to the Asian lineage,traced to Timor-Leste,where an endemic CHIKV outbreak had been reported in January 2024,consistent with the patient's travel history.Finally,we analyzed genetic evolutionary trends and amino acid site variations.This study highlights the iden-tification of a CHIKV infection origin using direct whole-blood metatranscriptomic sequencing,a valuable method for rapidly sequencing low viral-load samples.
基金the China Postdoctoral Science Foundation(2017M622795)the Science,Technology and Innovation Commission of Shenzhen Municipality(JCYJ20180507183628543)the Fundamental Research Funds for the Central Universities(2662018PY025 and 2662017PY105)。
文摘The brain of the domestic pig(Sus scrofa domesticus)has drawn considerable attention due to its high similarities to that of humans.However,the cellular compositions of the pig brain(PB)remain elusive.Here we investigated the single-nucleus transcriptomic profiles of five regions of the PB(frontal lobe,parietal lobe,temporal lobe,occipital lobe,and hypothalamus)and identified 21 cell subpopulations.The cross-species comparison of mouse and pig hypothalamus revealed the shared and specific gene expression patterns at the single-cell resolution.Furthermore,we identified cell types and molecular pathways closely associated with neurological disorders,bridging the gap between gene mutations and pathogenesis.We reported,to our knowledge,the first single-cell atlas of domestic pig cerebral cortex and hypothalamus combined with a comprehensive analysis across species,providing extensive resources for future research regarding neural science,evolutionary developmental biology,and regenerative medicine.
基金supported by grants from the National Key R&D Program of China(Grant No.2017YFC0906501)。
文摘The importance of structural variants(SVs)for human phenotypes and diseases is now recognized.Although a variety of SV detection platforms and strategies that vary in sensitivity and specificity have been developed,few benchmarking procedures are available to confidently assess their performances in biological and clinical research.To facilitate the validation and application of these SV detection approaches,we established an Asian reference material by characterizing the genome of an Epstein-Barr virus(EBV)-immortalized B lymphocyte line along with identified benchmark regions and high-confidence SV calls.We established a high-confidence SV callset with 8938 SVs by integrating four alignment-based SV callers,including 109×Pacific Bio sciences(PacBio)continuous long reads(CLRs),22×PacBio circular consensus sequencing(CCS)reads,104×Oxford Nanopore Technologies(ONT)long reads,and 114×Bionano optical mapping platform,and one de novo assembly-based SV caller using CCS reads.A total of 544 randomly selected SVs were validated by PCR amplification and Sanger sequencing,demonstrating the robustness of our SV calls.Combining trio-binning-based haplotype assemblies,we established an SV benchmark for identifying false negatives and false positives by constructing the continuous high-confidence regions(CHCRs),which covered 1.46 gigabase pairs(Gb)and 6882 SVs supported by at least one diploid haplotype assembly.Establishing high-confidence SV calls for a benchmark sample that has been characterized by multiple technologies provides a valuable resource for investigating SVs in human biology,disease,and clinical research.
文摘We have recently pointed out how the existence of massive neutrinos would imply the possibility of forming very massive self-gravitating systems of neutrinos held in equilibrium by their Fermi pressure. In dealing with this question, the neutrinos have been assumed to be in a degenerate state and their Fermi temperature has been computed for a selected number of equilibrium configurations. All the
