Korean pine(Pinus koraiensis Siebold&Zucc.)in Northeastern China has been genetically improved to increase seed yields in addition to timber.To assess seed yield variability and select highly productive clones,14 ...Korean pine(Pinus koraiensis Siebold&Zucc.)in Northeastern China has been genetically improved to increase seed yields in addition to timber.To assess seed yield variability and select highly productive clones,14 cone,seed and nut traits were measured and analyzed.Variance analysis showed that all clones were signifi cantly different in various traits(P<0.01).Phenotypic coeffi cients of variation and repeatability of traits ranged from 9.1 to 34.4%and from 27.5 to 97.7%,respectively.Except for the cone layer and cone seed numbers,the other traits were positively or negatively correlated.Three principal components were identifi ed.Seed and nut traits were the most important traits in the fi rst principal component,and cone traits more important in the second.Using correlation and principal component analyses,cone number and other traits were selected to evaluate materials.Twenty-two clones were selected using a selection rate of 10%based on cone number independently or other combined traits.The genetic gain for diff erent traits ranged from 6.2 to 24.3%.The selected elite clones can supply seedlings for reforestation and the selection method can provide a theoretical basis for selection in other conifer species.展开更多
Genetic parameters were evaluated for growth and cone characteristics(tree height,diameter at breast height,volume,cone number,thousand seeds weight and single cone seeds weight)on 86 half-sib families of Pinus koraie...Genetic parameters were evaluated for growth and cone characteristics(tree height,diameter at breast height,volume,cone number,thousand seeds weight and single cone seeds weight)on 86 half-sib families of Pinus koraiensis aged 31 years.Analyses of variance revealed significant differences(p<0.001)in all growth and cone traits among families while no significant differences were detected among blocks and the interaction between blocks and families.The average family values for growth traits were 17.22 m,8.67 cm and 0.43 m^(3) for tree height,diameter at breast height and volume,respectively.The average cone number,thousand seeds weight and single cone seeds weight were 17.57,748.91 g and 77.25 g,respectively.Genotypic additive variance and phenotypic variances ranged from 0.00009 to 3.820 and from 0.0005 to 23.066,while genotypic and phenotypic coefficients of variation ranged from 2.693%to 37.196%and 4.963%to 60.595%,respectively.Heritability at the individual and family level ranged from 0.152 to 0.215 and 0.611 to 0.862,respectively.Growth traits were significantly positively correlated with each other,but cone traits showed a weak correlation with growth traits.Based on 10% selection rate,nine families each were selected as elite materials in terms of high performance in volume and cone numbers,with 22.16%and 43.82%genetic gain in volume and cone number,respectively.These results provide beneficial information to select excellent families and establish orchards of P.koraiensis from improved seeds.展开更多
Magnesium(Mg)is the fourth most abundant element in the human body and is important in terms of specific osteogenesis functions.Here,we provide a comprehensive review of the use of magnesium-based biomaterials(MBs)in ...Magnesium(Mg)is the fourth most abundant element in the human body and is important in terms of specific osteogenesis functions.Here,we provide a comprehensive review of the use of magnesium-based biomaterials(MBs)in bone reconstruction.We review the history of MBs and their excellent biocompatibility,biodegradability and osteopromotive properties,highlighting them as candidates for a new generation of biodegradable orthopedic implants.In particular,the results reported in the field-specific literature(280 articles)in recent decades are dissected with respect to the extensive variety of MBs for orthopedic applications,including Mg/Mg alloys,bioglasses,bioceramics,and polymer materials.We also summarize the osteogenic mechanism of MBs,including a detailed section on the physiological process,namely,the enhanced osteogenesis,promotion of osteoblast adhesion and motility,immunomodulation,and enhanced angiogenesis.Moreover,the merits and limitations of current bone grafts and substitutes are compared.The objective of this review is to reveal the strong potential of MBs for their use as agents in bone repair and regeneration and to highlight issues that impede their clinical translation.Finally,the development and challenges of MBs for transplanted orthopedic materials are discussed.展开更多
This paper made analysis and evaluation of nutritional components and functional components of different Pueraria lobata roots. Nutritional components mainly include water,ash content,fat,reducing sugar,starch and cel...This paper made analysis and evaluation of nutritional components and functional components of different Pueraria lobata roots. Nutritional components mainly include water,ash content,fat,reducing sugar,starch and cellulose; functional components mainly include flavone and polyphenol. Pueraria lobata root No. 1 has highest ash content,flavone,and polyphenol but lowest fat,so it is suitable for using as medical Pueraria lobata root resource. Pueraria lobata root No. 5 has starch content as high as 64. 43%,and is recommended using as vegetable and processing into Pueraria lobata powder. Pueraria lobata root No. 5 has cellulose content as high as 17. 79% and is recommended processing into Pueraria lobata tablets. Through comparison of nutritional and functional components of different Pueraria lobata roots,it is intended to provide reference for variety selection,breeding,production and processing of Pueraria lobata roots.展开更多
Ultrasound can be considered a mechanical wave for both clinical diagnostic and therapeutic purposes on the basis of its good penetrability and directivity while spreading in solid organs or tissues without any ionizi...Ultrasound can be considered a mechanical wave for both clinical diagnostic and therapeutic purposes on the basis of its good penetrability and directivity while spreading in solid organs or tissues without any ionizing radiation.As a powerful form of energy,ultrasound,is used for deep-tissue therapy with different sonication parameters.The feasibility of minimally invasive or noninvasive acoustic treatment of a variety of diseases,such as hypertension,arrhythmia,hypertrophic cardiomyopathy,and myocardial infraction,is being explored in animal experiments and clinical trials.In this review,we summarize the biomedical effects of acoustic intervention in experimental and clinical studies,current challenges,and the potential of ultrasound for cardiovascular disease therapy.展开更多
Composition teaching is an important part of Chinese teaching in middle schools and an important part of Chinese teaching. The students writing level is an important symbol to measure the quality of Chinese teaching. ...Composition teaching is an important part of Chinese teaching in middle schools and an important part of Chinese teaching. The students writing level is an important symbol to measure the quality of Chinese teaching. However, the quality of many students compositions is hardly satisfactory to our teachers. Composition has become a major difficulty for students to learn Chinese and a major difficulty for teachers to teach Chinese. After years of teaching practice, I found that only by stimulating students interest in learning can the quality of students learning be improved. Then, how to stimulate students interest in composition class, I think we should clarify the practical value of composition, so that students can make clear the purpose of composition. On this basis, stimulate students to discover "heart" life and write down their true feelings about "heart" life.展开更多
Objective: to analyze the effect of optimizing emergency nursing process on the rescue of patients with chest pain. Methods: a total of 62 patients with emergency chest pain admitted to our hospital from July 2020 to ...Objective: to analyze the effect of optimizing emergency nursing process on the rescue of patients with chest pain. Methods: a total of 62 patients with emergency chest pain admitted to our hospital from July 2020 to June 2021 were randomly selected. They were divided into the control group (even number of beds) with emergency nursing and the observation group (odd number of beds) with optimized emergency nursing process. There were 31 patients in each group, and the effects after the intervention were compared. Results: after the intervention, the time spent in the rescue process of the patients in the observation group was lower than that of the control group (P < 0.05), and the hospitalization time was lower than that of the control group (P < 0.05). Meanwhile, the incidence of adverse events was also lower than that of the control group (P < 0.05), and the PaCO2 level of the patients in the observation group was significantly lower than that of the control group (P < 0.05);The PaO2 level in the observation group was higher than that in the control group (P < 0.05);The quality of life score was higher than that of the control group (P < 0.05);The satisfaction was higher than that of the control group (P < 0.05);The rescue success was also higher than that of the control group (P < 0.05). Conclusion: for emergency chest pain patients, the application of optimized emergency nursing process has a significant effect in improving the rescue success rate, reducing the incidence of adverse events, reducing the length of hospital stay, and improving the quality of life.展开更多
Implant-associated Staphylococcus aureus(S.aureus)osteomyelitis is a severe challenge in orthopedics.While antibiotic-loaded bone cement is a standardized therapeutic approach for S.aureus osteomyelitis,it falls short...Implant-associated Staphylococcus aureus(S.aureus)osteomyelitis is a severe challenge in orthopedics.While antibiotic-loaded bone cement is a standardized therapeutic approach for S.aureus osteomyelitis,it falls short in eradicating Staphylococcus abscess communities(SACs)and bacteria within osteocyte-lacuna canalicular network(OLCN)and repairing bone defects.To address limitations,we developed a borosilicate bioactive glass(BSG)combined with ferroferric oxide(Fe_(3)O_(4))magnetic scaffold to enhance antibacterial efficacy and bone repair capabilities.We conducted comprehensive assessments of the osteoinductive,immunomodulatory,antibacterial properties,and thermal response of this scaffold,with or without an alternating magnetic field(AMF).Utilizing a well-established implant-related S.aureus tibial infection rabbit model,we evaluated its antibacterial performance in vivo.RNA transcriptome sequencing demonstrated that BSG+5%Fe_(3)O_(4)enhanced the immune response to bacteria and promoted osteogenic differentiation and mineralization of MSCs.Notably,BSG+5%Fe_(3)O_(4)upregulated gene expression of NOD-like receptor and TNF pathway in MSCs,alongside increased the expression of osteogenic factors(RUNX2,ALP and OCN)in vitro.Flow cytometry on macrophage exhibited a polarization effect towards M2,accompanied by upregulation of anti-inflammatory genes(TGF-β1 and IL-1Ra)and downregulation of pro-inflammatory genes(IL-6 and IL-1β)among macrophages.In vivo CT imaging revealed the absence of osteolysis and periosteal response in rabbits treated with BSG+5%Fe_(3)O_(4)+AMF at 42 days.Histological analysis indicated complete controls of SACs and bacteria within OLCN by day 42,along with new bone formation,signifying effective control of S.aureus osteomyelitis.Further investigations will focus on the in vivo biosafety and biological mechanism of this scaffold within infectious microenvironment.展开更多
Bone infection is a catastrophe in clinical orthopedics.Despite being the standard therapy for osteomyelitis,antibiotic-loaded polymethyl methacrylate(PMMA)cement has low efficiency against bacteria in biofilms.Furthe...Bone infection is a catastrophe in clinical orthopedics.Despite being the standard therapy for osteomyelitis,antibiotic-loaded polymethyl methacrylate(PMMA)cement has low efficiency against bacteria in biofilms.Furthermore,high-dose antibiotic-loaded implants carry risks of bacterial resistance,tissue toxicity,and impairment of local tissue healing.By incorporating borosilicate bioactive glass(BSG)into low-dose gentamicin sulfate(GS)-loaded PMMA cement,an intelligent strategy that synergistically eradicates bacteria and sequen-tially promotes osseointegration,was devised.Results showed that BSG did not compromises the handling properties of the cement,but actually endowed it with an ionic and alkaline microenvironment,thereby damaging the integrity of bacterial cell walls and membranes,inhibiting ATP synthesis by disrupting the res-piratory chain in cell membranes and glycogen metabolism,and elevating reactive oxygen species(ROS)levels by weakening antioxidant components(peroxisomes and carotenoids).These antibacterial characteristics of BSG synergistically reinforced the effectiveness of GS,which was far below the actual clinical dosage,achieving efficient bacterial killing and biofilm clearance by binding to the 30S subunit of ribosomes.Furthermore,the released GS and the ionic and alkaline microenvironment from the implants fostered the osteogenic activity of hBMSCs in vitro and coordinately enhanced osseointegration in vivo.Collectively,this study underscores that BSG incorporation offers a promising strategy for reducing antibiotic dosage while simultaneously enhancing the antibacterial activity and osteogenesis of implants.This approach holds potential for resolving the conflict be-tween bacterial resistance and bone infection.展开更多
Fly ash,as a byproduct of municipal solid waste incineration,contains several kinds of pollutants,especially dissolvable salts that cause a severe challenge for fly ash disposal.Washing combined with cement kiln co-pr...Fly ash,as a byproduct of municipal solid waste incineration,contains several kinds of pollutants,especially dissolvable salts that cause a severe challenge for fly ash disposal.Washing combined with cement kiln co-processing for fly ash disposal has been applied in China.After washing,the wastewater was evaporated to produce fly ash salt(FAS).In this study,FAS was mixed the KCl and LiCl to be used as molten chloride salt for energy storage material.Twenty-three types of molten salts with various ratios of FAS-KCl-LiCl were evaluated.Thermophysical properties(melting point and latent heat)and thermal stability of these salts were characterized.The increase in FAS fraction decreased the latent heat of molten salts.Among the tested samples,the best compatibility ratio of FAS:KCl:LiCl was 10:50:40(%,in weight),with latent heat of 108.7 J/g and melting point of 333℃.This molten salt also showed good thermal stability after 1–13 h of thermal experiments,and the mass loss was less than 2%after 5 heating cycles at 600℃.By corrosion test,FAS-KCl-LiCl(10:50:40,%in weight)could be more safely used in vessels made of nickel-based alloy,but it might cause corrosion risk for stainless steel.展开更多
Correction to:Waste Disposal&Sustainable Energy(2022)4:193-203 https:/doi.0rg/10.1007/s42768-022-00110-6 The section‘Conflict of Interest'has been amended:‘Mi Yan is the Young Editorial Board member of Waste...Correction to:Waste Disposal&Sustainable Energy(2022)4:193-203 https:/doi.0rg/10.1007/s42768-022-00110-6 The section‘Conflict of Interest'has been amended:‘Mi Yan is the Young Editorial Board member of Waste Disposal&Sustainable Energy.'The revised‘Conflict of Interest'is as follows:Mi Yan is the Young Editorial Board member of Waste Disposal&Sustainable Energy.The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.展开更多
A variety of pyrazole derivatives containing 1,3,4-thiadiazole moiety were synthesized under microwave irradiation, and their structures were confirmed by 1H NMR and HRMS. They were evaluated for herbicidal and antifu...A variety of pyrazole derivatives containing 1,3,4-thiadiazole moiety were synthesized under microwave irradiation, and their structures were confirmed by 1H NMR and HRMS. They were evaluated for herbicidal and antifungal activities, and the results indicated that two compounds with a phenyl group （6a） and 4-tert-butylphe- nyl group （6n） possess good herbicidal activity for dicotyledon Brassica campestris and Raphanus sativus with the inhibition of 90% for root and 80%-90% for stalk at 100 ppm respectively. The structure-activity relationship of compounds 6a and 6n was also studied by density function theory method.展开更多
The rising production of sewage sludge,characterized by high organic content and excessive heavy metals,necessitates an effective treatment method.This study investigated the production of syngas and the migration and...The rising production of sewage sludge,characterized by high organic content and excessive heavy metals,necessitates an effective treatment method.This study investigated the production of syngas and the migration and transformation behavior of heavy metals such as Zn,Ni,Cr,Cu,and As during supercritical water gasification(SCWG)of sewage sludge.The experiments were conducted without or with alkaline additives at temperatures between 380 to 420℃ and retention time from 15 to 60 min.The results revealed that the highest syngas yield reached 10.9 mol/kg with an H2 concentration of 44.7% at 420℃ and 60 min.In this process,heavy metals were effectively immobilized and converted into a more stable form,whereas higher temperatures and longer retention time enhanced this effect.The introduction of alkaline additives(NaOH,KOH,Ca(OH)_(2),Na_(2)CO_(3),and K_(2)CO_(3))led to the redistribution of heavy metals,further promoting the stabilization of Zn,Cr,and Cu.An environmental risk assessment showed that SCWG could significantly lower the risk associated with heavy metals to a low or negligible level.展开更多
基金the Forestry Science Technology and Development Project(KJZXSA2019046)Fundamental Research Funds for the Central Universities(2572017DA02).
文摘Korean pine(Pinus koraiensis Siebold&Zucc.)in Northeastern China has been genetically improved to increase seed yields in addition to timber.To assess seed yield variability and select highly productive clones,14 cone,seed and nut traits were measured and analyzed.Variance analysis showed that all clones were signifi cantly different in various traits(P<0.01).Phenotypic coeffi cients of variation and repeatability of traits ranged from 9.1 to 34.4%and from 27.5 to 97.7%,respectively.Except for the cone layer and cone seed numbers,the other traits were positively or negatively correlated.Three principal components were identifi ed.Seed and nut traits were the most important traits in the fi rst principal component,and cone traits more important in the second.Using correlation and principal component analyses,cone number and other traits were selected to evaluate materials.Twenty-two clones were selected using a selection rate of 10%based on cone number independently or other combined traits.The genetic gain for diff erent traits ranged from 6.2 to 24.3%.The selected elite clones can supply seedlings for reforestation and the selection method can provide a theoretical basis for selection in other conifer species.
基金We are grateful to the Applied Technology Research and Development Planned Program of Heilongjiang Province(GA19B201-4)for supporting this research.
文摘Genetic parameters were evaluated for growth and cone characteristics(tree height,diameter at breast height,volume,cone number,thousand seeds weight and single cone seeds weight)on 86 half-sib families of Pinus koraiensis aged 31 years.Analyses of variance revealed significant differences(p<0.001)in all growth and cone traits among families while no significant differences were detected among blocks and the interaction between blocks and families.The average family values for growth traits were 17.22 m,8.67 cm and 0.43 m^(3) for tree height,diameter at breast height and volume,respectively.The average cone number,thousand seeds weight and single cone seeds weight were 17.57,748.91 g and 77.25 g,respectively.Genotypic additive variance and phenotypic variances ranged from 0.00009 to 3.820 and from 0.0005 to 23.066,while genotypic and phenotypic coefficients of variation ranged from 2.693%to 37.196%and 4.963%to 60.595%,respectively.Heritability at the individual and family level ranged from 0.152 to 0.215 and 0.611 to 0.862,respectively.Growth traits were significantly positively correlated with each other,but cone traits showed a weak correlation with growth traits.Based on 10% selection rate,nine families each were selected as elite materials in terms of high performance in volume and cone numbers,with 22.16%and 43.82%genetic gain in volume and cone number,respectively.These results provide beneficial information to select excellent families and establish orchards of P.koraiensis from improved seeds.
基金financial support from the National Natural Science Foundation of China(No.81672230)the Natural Science Foundation of Chongqing(No.cstc2020jcyjmsxm2234)+1 种基金the Top-notch Young Talent Project of Chongqing Traditional Chinese Medicine Hospital(No.CQSZYY2020008)the Chongqing Graduate Research Innovation Project(No.CYS20199)。
文摘Magnesium(Mg)is the fourth most abundant element in the human body and is important in terms of specific osteogenesis functions.Here,we provide a comprehensive review of the use of magnesium-based biomaterials(MBs)in bone reconstruction.We review the history of MBs and their excellent biocompatibility,biodegradability and osteopromotive properties,highlighting them as candidates for a new generation of biodegradable orthopedic implants.In particular,the results reported in the field-specific literature(280 articles)in recent decades are dissected with respect to the extensive variety of MBs for orthopedic applications,including Mg/Mg alloys,bioglasses,bioceramics,and polymer materials.We also summarize the osteogenic mechanism of MBs,including a detailed section on the physiological process,namely,the enhanced osteogenesis,promotion of osteoblast adhesion and motility,immunomodulation,and enhanced angiogenesis.Moreover,the merits and limitations of current bone grafts and substitutes are compared.The objective of this review is to reveal the strong potential of MBs for their use as agents in bone repair and regeneration and to highlight issues that impede their clinical translation.Finally,the development and challenges of MBs for transplanted orthopedic materials are discussed.
基金Supported by Special Project for Construction of Modern Agricultural Industrial Technology System of Ministry of Agriculture(nycytx-35-02-06)Hubei Public Science and Technology Program(2014BBA183)
文摘This paper made analysis and evaluation of nutritional components and functional components of different Pueraria lobata roots. Nutritional components mainly include water,ash content,fat,reducing sugar,starch and cellulose; functional components mainly include flavone and polyphenol. Pueraria lobata root No. 1 has highest ash content,flavone,and polyphenol but lowest fat,so it is suitable for using as medical Pueraria lobata root resource. Pueraria lobata root No. 5 has starch content as high as 64. 43%,and is recommended using as vegetable and processing into Pueraria lobata powder. Pueraria lobata root No. 5 has cellulose content as high as 17. 79% and is recommended processing into Pueraria lobata tablets. Through comparison of nutritional and functional components of different Pueraria lobata roots,it is intended to provide reference for variety selection,breeding,production and processing of Pueraria lobata roots.
文摘Ultrasound can be considered a mechanical wave for both clinical diagnostic and therapeutic purposes on the basis of its good penetrability and directivity while spreading in solid organs or tissues without any ionizing radiation.As a powerful form of energy,ultrasound,is used for deep-tissue therapy with different sonication parameters.The feasibility of minimally invasive or noninvasive acoustic treatment of a variety of diseases,such as hypertension,arrhythmia,hypertrophic cardiomyopathy,and myocardial infraction,is being explored in animal experiments and clinical trials.In this review,we summarize the biomedical effects of acoustic intervention in experimental and clinical studies,current challenges,and the potential of ultrasound for cardiovascular disease therapy.
文摘Composition teaching is an important part of Chinese teaching in middle schools and an important part of Chinese teaching. The students writing level is an important symbol to measure the quality of Chinese teaching. However, the quality of many students compositions is hardly satisfactory to our teachers. Composition has become a major difficulty for students to learn Chinese and a major difficulty for teachers to teach Chinese. After years of teaching practice, I found that only by stimulating students interest in learning can the quality of students learning be improved. Then, how to stimulate students interest in composition class, I think we should clarify the practical value of composition, so that students can make clear the purpose of composition. On this basis, stimulate students to discover "heart" life and write down their true feelings about "heart" life.
文摘Objective: to analyze the effect of optimizing emergency nursing process on the rescue of patients with chest pain. Methods: a total of 62 patients with emergency chest pain admitted to our hospital from July 2020 to June 2021 were randomly selected. They were divided into the control group (even number of beds) with emergency nursing and the observation group (odd number of beds) with optimized emergency nursing process. There were 31 patients in each group, and the effects after the intervention were compared. Results: after the intervention, the time spent in the rescue process of the patients in the observation group was lower than that of the control group (P < 0.05), and the hospitalization time was lower than that of the control group (P < 0.05). Meanwhile, the incidence of adverse events was also lower than that of the control group (P < 0.05), and the PaCO2 level of the patients in the observation group was significantly lower than that of the control group (P < 0.05);The PaO2 level in the observation group was higher than that in the control group (P < 0.05);The quality of life score was higher than that of the control group (P < 0.05);The satisfaction was higher than that of the control group (P < 0.05);The rescue success was also higher than that of the control group (P < 0.05). Conclusion: for emergency chest pain patients, the application of optimized emergency nursing process has a significant effect in improving the rescue success rate, reducing the incidence of adverse events, reducing the length of hospital stay, and improving the quality of life.
基金support from National Key R&D Program of China(2023YFC2416900and 2021YFC2400500)The International Postdoctoral Exchange Fellowship Program of Chongqing(2021JLPY004)+4 种基金The Fellowship of China Postdoctoral Science Foundation(2021M693758)National Natural Science Foundation of China(U22A20357,52072398and 32161160327)Natural Science Foundation Postdoctoral Science Foundation Project of Chongqing(cstc2021jcyj-bsh0019)Natural Science Foundation of Chongqing(cstc2021jcyj-msxmX0134)Shenzhen Science and Technology Program(JCYJ20230807140714030)。
文摘Implant-associated Staphylococcus aureus(S.aureus)osteomyelitis is a severe challenge in orthopedics.While antibiotic-loaded bone cement is a standardized therapeutic approach for S.aureus osteomyelitis,it falls short in eradicating Staphylococcus abscess communities(SACs)and bacteria within osteocyte-lacuna canalicular network(OLCN)and repairing bone defects.To address limitations,we developed a borosilicate bioactive glass(BSG)combined with ferroferric oxide(Fe_(3)O_(4))magnetic scaffold to enhance antibacterial efficacy and bone repair capabilities.We conducted comprehensive assessments of the osteoinductive,immunomodulatory,antibacterial properties,and thermal response of this scaffold,with or without an alternating magnetic field(AMF).Utilizing a well-established implant-related S.aureus tibial infection rabbit model,we evaluated its antibacterial performance in vivo.RNA transcriptome sequencing demonstrated that BSG+5%Fe_(3)O_(4)enhanced the immune response to bacteria and promoted osteogenic differentiation and mineralization of MSCs.Notably,BSG+5%Fe_(3)O_(4)upregulated gene expression of NOD-like receptor and TNF pathway in MSCs,alongside increased the expression of osteogenic factors(RUNX2,ALP and OCN)in vitro.Flow cytometry on macrophage exhibited a polarization effect towards M2,accompanied by upregulation of anti-inflammatory genes(TGF-β1 and IL-1Ra)and downregulation of pro-inflammatory genes(IL-6 and IL-1β)among macrophages.In vivo CT imaging revealed the absence of osteolysis and periosteal response in rabbits treated with BSG+5%Fe_(3)O_(4)+AMF at 42 days.Histological analysis indicated complete controls of SACs and bacteria within OLCN by day 42,along with new bone formation,signifying effective control of S.aureus osteomyelitis.Further investigations will focus on the in vivo biosafety and biological mechanism of this scaffold within infectious microenvironment.
基金supported by the National Key R&D Program of China(Grant No.2023YFC2416900)the National Natural Science Foundation of China(Grant No.U22A20357,52072398,82102584 and 32161160327)+1 种基金Shenzhen Science and Technology Program(Grant No.JCYJ20230807140714030,JCYJ20220818101613028,and JSGGKQTD20210831174330015)Shenzhen Medical Research Found(B2302031).
文摘Bone infection is a catastrophe in clinical orthopedics.Despite being the standard therapy for osteomyelitis,antibiotic-loaded polymethyl methacrylate(PMMA)cement has low efficiency against bacteria in biofilms.Furthermore,high-dose antibiotic-loaded implants carry risks of bacterial resistance,tissue toxicity,and impairment of local tissue healing.By incorporating borosilicate bioactive glass(BSG)into low-dose gentamicin sulfate(GS)-loaded PMMA cement,an intelligent strategy that synergistically eradicates bacteria and sequen-tially promotes osseointegration,was devised.Results showed that BSG did not compromises the handling properties of the cement,but actually endowed it with an ionic and alkaline microenvironment,thereby damaging the integrity of bacterial cell walls and membranes,inhibiting ATP synthesis by disrupting the res-piratory chain in cell membranes and glycogen metabolism,and elevating reactive oxygen species(ROS)levels by weakening antioxidant components(peroxisomes and carotenoids).These antibacterial characteristics of BSG synergistically reinforced the effectiveness of GS,which was far below the actual clinical dosage,achieving efficient bacterial killing and biofilm clearance by binding to the 30S subunit of ribosomes.Furthermore,the released GS and the ionic and alkaline microenvironment from the implants fostered the osteogenic activity of hBMSCs in vitro and coordinately enhanced osseointegration in vivo.Collectively,this study underscores that BSG incorporation offers a promising strategy for reducing antibiotic dosage while simultaneously enhancing the antibacterial activity and osteogenesis of implants.This approach holds potential for resolving the conflict be-tween bacterial resistance and bone infection.
基金support from the International Cooperation Project of Zhejiang Province(No.2019C04026)National Natural Science Foundation,China(No.51976196).
文摘Fly ash,as a byproduct of municipal solid waste incineration,contains several kinds of pollutants,especially dissolvable salts that cause a severe challenge for fly ash disposal.Washing combined with cement kiln co-processing for fly ash disposal has been applied in China.After washing,the wastewater was evaporated to produce fly ash salt(FAS).In this study,FAS was mixed the KCl and LiCl to be used as molten chloride salt for energy storage material.Twenty-three types of molten salts with various ratios of FAS-KCl-LiCl were evaluated.Thermophysical properties(melting point and latent heat)and thermal stability of these salts were characterized.The increase in FAS fraction decreased the latent heat of molten salts.Among the tested samples,the best compatibility ratio of FAS:KCl:LiCl was 10:50:40(%,in weight),with latent heat of 108.7 J/g and melting point of 333℃.This molten salt also showed good thermal stability after 1–13 h of thermal experiments,and the mass loss was less than 2%after 5 heating cycles at 600℃.By corrosion test,FAS-KCl-LiCl(10:50:40,%in weight)could be more safely used in vessels made of nickel-based alloy,but it might cause corrosion risk for stainless steel.
文摘Correction to:Waste Disposal&Sustainable Energy(2022)4:193-203 https:/doi.0rg/10.1007/s42768-022-00110-6 The section‘Conflict of Interest'has been amended:‘Mi Yan is the Young Editorial Board member of Waste Disposal&Sustainable Energy.'The revised‘Conflict of Interest'is as follows:Mi Yan is the Young Editorial Board member of Waste Disposal&Sustainable Energy.The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
文摘A variety of pyrazole derivatives containing 1,3,4-thiadiazole moiety were synthesized under microwave irradiation, and their structures were confirmed by 1H NMR and HRMS. They were evaluated for herbicidal and antifungal activities, and the results indicated that two compounds with a phenyl group （6a） and 4-tert-butylphe- nyl group （6n） possess good herbicidal activity for dicotyledon Brassica campestris and Raphanus sativus with the inhibition of 90% for root and 80%-90% for stalk at 100 ppm respectively. The structure-activity relationship of compounds 6a and 6n was also studied by density function theory method.
基金the financial support from the National Natural Science Foundation of China(No.52250410339)the“Pioneer”and“Leading Goose”R&D Program of Zhejiang(China)(No.2024C03129).
文摘The rising production of sewage sludge,characterized by high organic content and excessive heavy metals,necessitates an effective treatment method.This study investigated the production of syngas and the migration and transformation behavior of heavy metals such as Zn,Ni,Cr,Cu,and As during supercritical water gasification(SCWG)of sewage sludge.The experiments were conducted without or with alkaline additives at temperatures between 380 to 420℃ and retention time from 15 to 60 min.The results revealed that the highest syngas yield reached 10.9 mol/kg with an H2 concentration of 44.7% at 420℃ and 60 min.In this process,heavy metals were effectively immobilized and converted into a more stable form,whereas higher temperatures and longer retention time enhanced this effect.The introduction of alkaline additives(NaOH,KOH,Ca(OH)_(2),Na_(2)CO_(3),and K_(2)CO_(3))led to the redistribution of heavy metals,further promoting the stabilization of Zn,Cr,and Cu.An environmental risk assessment showed that SCWG could significantly lower the risk associated with heavy metals to a low or negligible level.
