The effect of H2O2 pretreatment on Cd tolerance and translocation of rice seedlings were studied using two rice cultivars （N07-6 and N07-63） differing in Cd tolerance. The contents of malondialdehyde （MDA）, reduce...The effect of H2O2 pretreatment on Cd tolerance and translocation of rice seedlings were studied using two rice cultivars （N07-6 and N07-63） differing in Cd tolerance. The contents of malondialdehyde （MDA）, reduced glutathione （GSH）, non-protein thiols （NPT）, phytochelatins （PCs） and the activity of glutathione S-transferase （GST） were compared between the two cultivars exposed to various treatments. The results showed that 50 μmol/L Cd exposure significantly inhibited rice growth, enhanced the production of GSH, NPT, PCs and MDA, and increased the activity of GST, and there were significant differences between the two cultivars. More Cd was transported into the shoot of N07-6. The H2O2 pretreatment alleviated Cd toxicity by further increasing GSH, NPT and PCs contents, as well as the GST activity in roots. The increase degrees of these parameters in N07-63 were higher than those in N07-6, suggesting that the tolerance of N07-63 was enhanced more significantly than N07-6. Hydrogen peroxide reduced Cd translocation to rice shoot but affected the Cd content in root differently. From the above results, it may be speculated that there were remarkable differences in the Cd detoxification and response to the H2O2 pretreatment between the two cultivars.展开更多
The clearance of apoptotic cell debris,containing professional phagocytosis and non-professional phagocytosis,is essential for maintaining the homeostasis of healthy tissues.Here,we discovered that endothelial cells c...The clearance of apoptotic cell debris,containing professional phagocytosis and non-professional phagocytosis,is essential for maintaining the homeostasis of healthy tissues.Here,we discovered that endothelial cells could engulf apoptotic cell debris in atherosclerotic plaque.Single-cell RNA sequencing(RNA-seq)has revealed a unique endothelial cell subpopulation in atherosclerosis,which was strongly associated with vascular injury-related pathways.Moreover,integrated analysis of three vascular injury-related RNA-seq datasets showed that the expression of scavenger receptor class B type 1(SR-B1)was up-regulated and specifically enriched in the phagocytosis pathway under vascular injury circumstances.Single-cell RNA-seq and bulk RNA-seq indicate that SR-B1 was highly expressed in a unique endothelial cell subpopulation of mouse aorta and strongly associated with the reorganization of cellular adherent junctions and cytoskeleton which were necessary for phagocytosis.Furthermore,SR-B1 was strongly required for endothelial cells to engulf apoptotic cell debris in atherosclerotic plaque of both mouse and human aorta.Overall,this study demonstrated that apoptotic cell debris could be engulfed by endothelial cells through SR-B1 and associated with the reorganization of cellular adherent junctions and cytoskeleton.展开更多
Purpose:This review aims to explore the history,research hotspots,and emerging trends of drug-eluting stents(DES)in the last two decades from the perspective of structural and temporal dynamics.Methods:Publications on...Purpose:This review aims to explore the history,research hotspots,and emerging trends of drug-eluting stents(DES)in the last two decades from the perspective of structural and temporal dynamics.Methods:Publications on DES were retrieved from WoSCC.The bibliometric tools including CiteSpace and HistCite were used to identify the historical features,the evolution of active topics,and emerging trends on the DES field.Results:In the last 20 years,the field of DES is still in the hot phase and there is a wide range of extensive scientific collaborations.In addition,active topics emerge in different periods,as evidenced by a total of 41 disciplines,511 keywords,and 1377 papers with citation bursts.Keyword clustering anchored five emerging research subfields,namely#0 dual antiplatelet therapy,#3 drug-coated balloon,#4 bifurcation,5#rotational atherectomy,and 6#quantitative flow ratio.The keyword alluvial map shows that the most persistent research concepts in this field are thrombosis,restenosis,etc.,and the emerging keywords are paclitaxel eluting balloon,coated balloon,drug-eluting balloon,etc.There are 7 recent research subfields anchored by reference clustering,namely#2 dual antiplatelet therapy,#4 drug-coated balloon,#5 peripheral artery disease,#8 fractional flow reserve,#10 bioresorbable vascular scaffold,#13 intravascular ultrasound,#14 biodegradable polymer.Conclusion:The findings based on the bibliometric studies provide the current status and trends in DES research and may help researchers to identify hot topics and explore new research directions in this field.展开更多
Exosomes are membrane vesicles that exist in pathological or normal cells and are considered to be specifically secreted,contain complex RNAs and proteins,participate in intercellular communication functions,and have ...Exosomes are membrane vesicles that exist in pathological or normal cells and are considered to be specifically secreted,contain complex RNAs and proteins,participate in intercellular communication functions,and have functions in organismal immune response,antigen presentation,cell migration,cell differentiation,and tumor invasion.In recent years,with the explosion of the development of minimally invasive diagnostics,the research of exosomes has been rapidly developed,and the number of articles published in this field has increased dramatically,and many articles summarize the published papers in a review-type analysis.In this paper,we use bibliometric analysis combined with theoretical analysis and pathfinding network scaling to visualize data and objectively count the development process,current research,and development trend of the corresponding field by analyzing the literature of the specified field.This paper presents the data visualization by analyzing the literature of the specified field to objectively count the development process,current research and development trend of the corresponding field.This paper presents a retrospective analysis and review of the articles on exosomes in the last 20 years(2001–2021)based on the bibliometric analysis method of CiteSpace software,while presenting the problems of current research and discussing the challenges encountered in the future,hoping to provide new ideas for the development of research in this field.展开更多
The progression of cardiovascular diseases such as atherosclerosis and myocardial infarction leads to serious vascular injury,highlighting the urgent need for targeted regenerative therapy.Extracellular vesicles(EVs)c...The progression of cardiovascular diseases such as atherosclerosis and myocardial infarction leads to serious vascular injury,highlighting the urgent need for targeted regenerative therapy.Extracellular vesicles(EVs)composed of a lipid bilayer containing nuclear and cytosolic materials are relevant to the progression of cardiovascular diseases.Moreover,EVs can deliver bioactive cargo in pathological cardiovascular and regulate the biological function of recipient cells,such as inflammation,proliferation,angiogenesis and polarization.However,because the targeting and bioactivity of natural EVs are subject to several limitations,bioengineered EVs have achieved wide advancements in biomedicine.Bioengineered EVs involve three main ways to acquire including(i)modification of the EVs after isolation;(ii)modification of producer cells before EVs’isolation;(iii)synthesize EVs using natural or modified cell membranes,and encapsulating drugs or bioactive molecules into EVs.In this review,we first summarize the cardiovascular injury-related disease and describe the role of different cells and EVs in vascular regeneration.We also discuss the application of bioengineered EVs from different producer cells to cardiovascular diseases.Finally,we summarize the surface modification on EVs which can specifically target abnormal cells in injured vascular.展开更多
基金the financial support from the Natural Science Foundation of China (Grant No. 30700479)the Ph.D. Programs Foundation of Ministry of Education, China (Grant No. 20090097110035)
文摘The effect of H2O2 pretreatment on Cd tolerance and translocation of rice seedlings were studied using two rice cultivars （N07-6 and N07-63） differing in Cd tolerance. The contents of malondialdehyde （MDA）, reduced glutathione （GSH）, non-protein thiols （NPT）, phytochelatins （PCs） and the activity of glutathione S-transferase （GST） were compared between the two cultivars exposed to various treatments. The results showed that 50 μmol/L Cd exposure significantly inhibited rice growth, enhanced the production of GSH, NPT, PCs and MDA, and increased the activity of GST, and there were significant differences between the two cultivars. More Cd was transported into the shoot of N07-6. The H2O2 pretreatment alleviated Cd toxicity by further increasing GSH, NPT and PCs contents, as well as the GST activity in roots. The increase degrees of these parameters in N07-63 were higher than those in N07-6, suggesting that the tolerance of N07-63 was enhanced more significantly than N07-6. Hydrogen peroxide reduced Cd translocation to rice shoot but affected the Cd content in root differently. From the above results, it may be speculated that there were remarkable differences in the Cd detoxification and response to the H2O2 pretreatment between the two cultivars.
基金the National Natural Science Foundation of China(No.12032007,31971242 to G.Wang)the Science and Technology Innovation Project of Jinfeng Laboratory,Chongqing,China(No.jfkyjf202203001 to G.Wang).
文摘The clearance of apoptotic cell debris,containing professional phagocytosis and non-professional phagocytosis,is essential for maintaining the homeostasis of healthy tissues.Here,we discovered that endothelial cells could engulf apoptotic cell debris in atherosclerotic plaque.Single-cell RNA sequencing(RNA-seq)has revealed a unique endothelial cell subpopulation in atherosclerosis,which was strongly associated with vascular injury-related pathways.Moreover,integrated analysis of three vascular injury-related RNA-seq datasets showed that the expression of scavenger receptor class B type 1(SR-B1)was up-regulated and specifically enriched in the phagocytosis pathway under vascular injury circumstances.Single-cell RNA-seq and bulk RNA-seq indicate that SR-B1 was highly expressed in a unique endothelial cell subpopulation of mouse aorta and strongly associated with the reorganization of cellular adherent junctions and cytoskeleton which were necessary for phagocytosis.Furthermore,SR-B1 was strongly required for endothelial cells to engulf apoptotic cell debris in atherosclerotic plaque of both mouse and human aorta.Overall,this study demonstrated that apoptotic cell debris could be engulfed by endothelial cells through SR-B1 and associated with the reorganization of cellular adherent junctions and cytoskeleton.
基金This work was supported in part by grants from the National Natural Science Foundation of China(12032007,31971242)Chongqing Research Program of Basic research and Frontier Technology(cstc2019jcyj-zdxmX0028)+1 种基金JinFeng Laboratory Foundation,Chongqing,China(jfkyjf202203001)Shanghai Clinical Research Center for Interventional Medicine(19MC1910300).
文摘Purpose:This review aims to explore the history,research hotspots,and emerging trends of drug-eluting stents(DES)in the last two decades from the perspective of structural and temporal dynamics.Methods:Publications on DES were retrieved from WoSCC.The bibliometric tools including CiteSpace and HistCite were used to identify the historical features,the evolution of active topics,and emerging trends on the DES field.Results:In the last 20 years,the field of DES is still in the hot phase and there is a wide range of extensive scientific collaborations.In addition,active topics emerge in different periods,as evidenced by a total of 41 disciplines,511 keywords,and 1377 papers with citation bursts.Keyword clustering anchored five emerging research subfields,namely#0 dual antiplatelet therapy,#3 drug-coated balloon,#4 bifurcation,5#rotational atherectomy,and 6#quantitative flow ratio.The keyword alluvial map shows that the most persistent research concepts in this field are thrombosis,restenosis,etc.,and the emerging keywords are paclitaxel eluting balloon,coated balloon,drug-eluting balloon,etc.There are 7 recent research subfields anchored by reference clustering,namely#2 dual antiplatelet therapy,#4 drug-coated balloon,#5 peripheral artery disease,#8 fractional flow reserve,#10 bioresorbable vascular scaffold,#13 intravascular ultrasound,#14 biodegradable polymer.Conclusion:The findings based on the bibliometric studies provide the current status and trends in DES research and may help researchers to identify hot topics and explore new research directions in this field.
基金the National Natural Science Foundation of China(12032007,31971242)Chongqing Research Program of Basic research and Frontier Technology(cstc2019jcyjzdxmX0028,cstc2020jscx-msxmX0132)。
文摘Exosomes are membrane vesicles that exist in pathological or normal cells and are considered to be specifically secreted,contain complex RNAs and proteins,participate in intercellular communication functions,and have functions in organismal immune response,antigen presentation,cell migration,cell differentiation,and tumor invasion.In recent years,with the explosion of the development of minimally invasive diagnostics,the research of exosomes has been rapidly developed,and the number of articles published in this field has increased dramatically,and many articles summarize the published papers in a review-type analysis.In this paper,we use bibliometric analysis combined with theoretical analysis and pathfinding network scaling to visualize data and objectively count the development process,current research,and development trend of the corresponding field by analyzing the literature of the specified field.This paper presents the data visualization by analyzing the literature of the specified field to objectively count the development process,current research and development trend of the corresponding field.This paper presents a retrospective analysis and review of the articles on exosomes in the last 20 years(2001–2021)based on the bibliometric analysis method of CiteSpace software,while presenting the problems of current research and discussing the challenges encountered in the future,hoping to provide new ideas for the development of research in this field.
基金supported by the National Natural Science Foundation of China[Grant Nos.12032007,31971242]to G.W.Chongqing Science and Technology Bureau[Grant No.cstc2019jcyj-zdxmX0028]to G.W.+1 种基金Chongqing Municipal Education Commission,China[Grant No.KYYJ202001]to G.W.Guizhou Provincial Science and Technology Projects[Grant No.Qiankehejichu(2018)1103]to J.H.
文摘The progression of cardiovascular diseases such as atherosclerosis and myocardial infarction leads to serious vascular injury,highlighting the urgent need for targeted regenerative therapy.Extracellular vesicles(EVs)composed of a lipid bilayer containing nuclear and cytosolic materials are relevant to the progression of cardiovascular diseases.Moreover,EVs can deliver bioactive cargo in pathological cardiovascular and regulate the biological function of recipient cells,such as inflammation,proliferation,angiogenesis and polarization.However,because the targeting and bioactivity of natural EVs are subject to several limitations,bioengineered EVs have achieved wide advancements in biomedicine.Bioengineered EVs involve three main ways to acquire including(i)modification of the EVs after isolation;(ii)modification of producer cells before EVs’isolation;(iii)synthesize EVs using natural or modified cell membranes,and encapsulating drugs or bioactive molecules into EVs.In this review,we first summarize the cardiovascular injury-related disease and describe the role of different cells and EVs in vascular regeneration.We also discuss the application of bioengineered EVs from different producer cells to cardiovascular diseases.Finally,we summarize the surface modification on EVs which can specifically target abnormal cells in injured vascular.
