[ Objective] This study was conducted to investigate the mechanism of Echinacea polysaccharide （EPS） in treatment of various bacterial infection and reduction of inflammation, so as to provide a theoretical basis fo...[ Objective] This study was conducted to investigate the mechanism of Echinacea polysaccharide （EPS） in treatment of various bacterial infection and reduction of inflammation, so as to provide a theoretical basis for clinic application of EPS. [ Method ] Nuclear protein extracted from six groups, the normal control group, the simple lipopolysaccharide （LPS） group and the EPS （ with concentrations of 50,100,200 and 500 μg/ml, respectively） ＋ LPS groups was subjected to SDS-PAGE electrophoresis, and pIkB-α protein contents in the extracts were analyzed by Coomassie brilliant blue （CBB） staining and Western-Blot method. [ Result] The simple LPS group showed the highest pIkB-α protein level, and in the EPS concentration range of 0 -200 μg/ml, the expression level of pIkB-α pro- tein was improved with the increase of EPS concentration. [ Conclusion The expression level of pIkB-α protein was improved under the simulation of IEC-6 by LPS, while EPS could effectively inhibit the expression of pIkB-α protein. The expression level of pIkB-α was the lowest in the LPS ＋500 μg/ml EPS group.展开更多
[Objective]The paper was to analyze the correlation between drug resistance and resistant genotypes of pathogenic Klebsiella peneumoniae from racoon dog to fluoroquinolones.[Method]The drug resistance and resistant ge...[Objective]The paper was to analyze the correlation between drug resistance and resistant genotypes of pathogenic Klebsiella peneumoniae from racoon dog to fluoroquinolones.[Method]The drug resistance and resistant genes of 24 strains of pathogenic K.peneumoniae from racoon dog to fluoroquinolones were detected by K-B drug susceptibility paper and PCR.[Result]Multiple drug-resistant strains were dominant among 24 strains of pathogenic K.peneumoniae,and the drug resistance to levofloxacin,enrofloxacin,ciprofloxacin and norfloxacin was 70.8%-79.2%;the strains resistant to 4,3 and 2 antibiotics were 58.3%,29.2%and 8.3%,respectively.The detection rates of drug-resistant genes TEM,gyrB,parC,parE,gyrA and SHV were 75%-100%,and the number of strains carrying 9,8,7,6,4 and 3 drug-resistant genes was 4.2%-41.7%.Comparison of drug-resistant genes and drug resistance showed that the coincidence rates of levofloxacin,enrofloxacin,ciprofloxacin and norfloxacin were 78.9%-100%.[Conclusion]There were certain correlation between drug resistance and resistant genotypes of 24 strains of pathogenic Klebsiella peneumo-niae to fluoroquinolones.展开更多
In order to isolate and identify the pathogenic bacteria causing dead chickens in a chicken farm in Qinhuangdao area,the liver,heart and other organs of dead chickens suspected of salmonella disease were collected ase...In order to isolate and identify the pathogenic bacteria causing dead chickens in a chicken farm in Qinhuangdao area,the liver,heart and other organs of dead chickens suspected of salmonella disease were collected aseptically,and streaked on SS agar medium and chromagar medium.Transparent colonies were observed on SS agar medium,and purple and transparent colonies on CAS medium.The isolate was conducted purification,staining microscopy,biochemical tests,and 16 S rRNA sequence analysis,and the results showed that four strains of the isolated bacte-ria were salmonella.The 16 S rRNA sequence analysis of four strains of salmonella showed that the isolates shared more than 99%homology.Drug susceptibility test was performed using paper method,and the results showed that most of the strains were resistant to tilmicosin,cefradine and sul-famethoxazole,but were sensitive to ceftriaxone.展开更多
[Objective]The paper was to determine the pathogen causing fox pneumonia in a breeding factory in Changli County.[Method]Through autopsy,a dominant strain was isolated from the lung of dead foxes,which was then perfor...[Objective]The paper was to determine the pathogen causing fox pneumonia in a breeding factory in Changli County.[Method]Through autopsy,a dominant strain was isolated from the lung of dead foxes,which was then performed Gram staining,16 S rRNA sequence analysis and biochemical identification.[Result]The strain was negative in Gram staining,and was identified as E.coli through 16 S rRNA sequence analysis and biochemical identification.Drug susceptibility test was conducted using 15 kinds of drug susceptibility papers.The E.coli was sensitive to florfenicol,enrofloxacin,ceftriaxone,norfloxacin;intermediately sensitive to amikacin,gentamicin;and strongly resistant to penicillin,ampicillin,cefradine,sulfamethoxazole,lincomycin,streptomycin and amoxicillin.[Conclusion]It is difficult to treat E.coli causing fox pneumonia with traditional antibiotics clinically.展开更多
基金Supported by Natural Science Foundation of China(31472230)Natural Science Foundation of Hebei Province(C2014407068)Fund from Science and Technology Department of Hebei Province(NO.14966610D)
文摘[ Objective] This study was conducted to investigate the mechanism of Echinacea polysaccharide （EPS） in treatment of various bacterial infection and reduction of inflammation, so as to provide a theoretical basis for clinic application of EPS. [ Method ] Nuclear protein extracted from six groups, the normal control group, the simple lipopolysaccharide （LPS） group and the EPS （ with concentrations of 50,100,200 and 500 μg/ml, respectively） ＋ LPS groups was subjected to SDS-PAGE electrophoresis, and pIkB-α protein contents in the extracts were analyzed by Coomassie brilliant blue （CBB） staining and Western-Blot method. [ Result] The simple LPS group showed the highest pIkB-α protein level, and in the EPS concentration range of 0 -200 μg/ml, the expression level of pIkB-α pro- tein was improved with the increase of EPS concentration. [ Conclusion The expression level of pIkB-α protein was improved under the simulation of IEC-6 by LPS, while EPS could effectively inhibit the expression of pIkB-α protein. The expression level of pIkB-α was the lowest in the LPS ＋500 μg/ml EPS group.
基金Supported by Project of Hebei Department of Science and Technology(18246629G)Project of Shijiazhuang Science and Technology Bureau(171500953A)Project of Hebei Department of Education(ZD2017234)
文摘[Objective]The paper was to analyze the correlation between drug resistance and resistant genotypes of pathogenic Klebsiella peneumoniae from racoon dog to fluoroquinolones.[Method]The drug resistance and resistant genes of 24 strains of pathogenic K.peneumoniae from racoon dog to fluoroquinolones were detected by K-B drug susceptibility paper and PCR.[Result]Multiple drug-resistant strains were dominant among 24 strains of pathogenic K.peneumoniae,and the drug resistance to levofloxacin,enrofloxacin,ciprofloxacin and norfloxacin was 70.8%-79.2%;the strains resistant to 4,3 and 2 antibiotics were 58.3%,29.2%and 8.3%,respectively.The detection rates of drug-resistant genes TEM,gyrB,parC,parE,gyrA and SHV were 75%-100%,and the number of strains carrying 9,8,7,6,4 and 3 drug-resistant genes was 4.2%-41.7%.Comparison of drug-resistant genes and drug resistance showed that the coincidence rates of levofloxacin,enrofloxacin,ciprofloxacin and norfloxacin were 78.9%-100%.[Conclusion]There were certain correlation between drug resistance and resistant genotypes of 24 strains of pathogenic Klebsiella peneumo-niae to fluoroquinolones.
基金Supported by The Third Batch Giant Plan of Hebei Province(180416H)
文摘In order to isolate and identify the pathogenic bacteria causing dead chickens in a chicken farm in Qinhuangdao area,the liver,heart and other organs of dead chickens suspected of salmonella disease were collected aseptically,and streaked on SS agar medium and chromagar medium.Transparent colonies were observed on SS agar medium,and purple and transparent colonies on CAS medium.The isolate was conducted purification,staining microscopy,biochemical tests,and 16 S rRNA sequence analysis,and the results showed that four strains of the isolated bacte-ria were salmonella.The 16 S rRNA sequence analysis of four strains of salmonella showed that the isolates shared more than 99%homology.Drug susceptibility test was performed using paper method,and the results showed that most of the strains were resistant to tilmicosin,cefradine and sul-famethoxazole,but were sensitive to ceftriaxone.
基金Supported by Project of Hebei Department of Science and Technology(18246629G)Project of Shijiazhuang Science and Technology Bureau(171500953A)Project of Hebei Department of Education(ZD2017234)
文摘[Objective]The paper was to determine the pathogen causing fox pneumonia in a breeding factory in Changli County.[Method]Through autopsy,a dominant strain was isolated from the lung of dead foxes,which was then performed Gram staining,16 S rRNA sequence analysis and biochemical identification.[Result]The strain was negative in Gram staining,and was identified as E.coli through 16 S rRNA sequence analysis and biochemical identification.Drug susceptibility test was conducted using 15 kinds of drug susceptibility papers.The E.coli was sensitive to florfenicol,enrofloxacin,ceftriaxone,norfloxacin;intermediately sensitive to amikacin,gentamicin;and strongly resistant to penicillin,ampicillin,cefradine,sulfamethoxazole,lincomycin,streptomycin and amoxicillin.[Conclusion]It is difficult to treat E.coli causing fox pneumonia with traditional antibiotics clinically.
