Medical stents are vital for treating vascular complications and restoring blood flow in millions of patients.Despite its widespread effectiveness,restenosis,driven by the complex interplay of cellular responses,remai...Medical stents are vital for treating vascular complications and restoring blood flow in millions of patients.Despite its widespread effectiveness,restenosis,driven by the complex interplay of cellular responses,remains a concern.This study investigated the reactions of vascular cells to nano/microscale wrinkle(nano-W and micro-W)patterns created on laser-textured nitinol(NiTi)surfaces by adjusting laser processing parameters,such as spot overlap ratio and line overlap ratio.Evaluation of topographical effects on endothelial and smooth muscle cells(SMCs)revealed diverse morphologies,proliferation rates,and gene expressions.Notably,microscale wrinkle patterns exhibited reduced monocyte adhesion and inflammation-related gene expression,demon-strating their potential applications in mitigating vascular complications after stent insertion.Additionally,an ex vivo metatarsal assay was utilized to bridge the gap between in vitro and in vivo studies,demonstrating enhanced angiogenesis on laser-textured NiTi surfaces.Laser-textured NiTi exhibits a guided formation process,empha-sizing their potential to promote swift endothelialization.These findings underscore the efficacy of laser texturing for tailored cellular interactions on metallic surfaces and offer valuable insights into optimizing biocompatibility and controlling cellular responses,which may pave the way for innovative advances in vascular care and contribute to the ongoing improvement of stent insertion.展开更多
Developing a universal culture platform that manipulates cell fate is one of the most important tasks in the investigation of the role of the cellular microenvironment.This study focuses on the application of topograp...Developing a universal culture platform that manipulates cell fate is one of the most important tasks in the investigation of the role of the cellular microenvironment.This study focuses on the application of topographical and electrical field stimuli to human myogenic precursor cell(hMPC)cultures to assess the influences of the adherent direction,proliferation,and differentiation,and induce preconditioning-induced therapeutic benefits.First,a topographical surface of commercially available culture dishes was achieved by femtosecond laser texturing.The detachable biphasic electrical current system was then applied to the hMPCs cultured on laser-textured culture dishes.Laser-textured topographies were remarkably effective in inducing the assembly of hMPC myotubes by enhancing the orientation of adherent hMPCs compared with flat surfaces.Furthermore,electrical field stimulation through laser-textured topographies was found to promote the expression of myogenic regulatory factors compared with nonstimulated cells.As such,we successfully demonstrated that the combined stimulation of topographical and electrical cues could effectively enhance the myogenic maturation of hMPCs in a surface spatial and electrical field-dependent manner,thus providing the basis for therapeutic strategies.展开更多
基金supported by a KIST project[2E32351,2E33122,2V09840-23-P023]the KU-KIST Graduate School of Converging Science and Technology Program+4 种基金supported by a National Research Foundation of Korea(NRF)grant funded by the Korean government(MSIT)[grant number RS-2023-00302145]supported by the Bio-cluster Industry Capacity Enhance-ment Project of Jeonbuk Technopark(JBTP)supported by the Korea Medical Device Development Fund grant funded by the Korean government(the Ministry of Science and ICT,the Ministry of Trade,Industry and Energy,the Ministry of Health&Wel-fare,and the Ministry of Food and Drug Safety)(NTIS Number:9991007189)supported by the Ministry of Trade,Industry,and Energy(MOTIE)of Korea through project No.P0022331supervised by the Korea Institute for Advance-ment of Technology(KIAT).
文摘Medical stents are vital for treating vascular complications and restoring blood flow in millions of patients.Despite its widespread effectiveness,restenosis,driven by the complex interplay of cellular responses,remains a concern.This study investigated the reactions of vascular cells to nano/microscale wrinkle(nano-W and micro-W)patterns created on laser-textured nitinol(NiTi)surfaces by adjusting laser processing parameters,such as spot overlap ratio and line overlap ratio.Evaluation of topographical effects on endothelial and smooth muscle cells(SMCs)revealed diverse morphologies,proliferation rates,and gene expressions.Notably,microscale wrinkle patterns exhibited reduced monocyte adhesion and inflammation-related gene expression,demon-strating their potential applications in mitigating vascular complications after stent insertion.Additionally,an ex vivo metatarsal assay was utilized to bridge the gap between in vitro and in vivo studies,demonstrating enhanced angiogenesis on laser-textured NiTi surfaces.Laser-textured NiTi exhibits a guided formation process,empha-sizing their potential to promote swift endothelialization.These findings underscore the efficacy of laser texturing for tailored cellular interactions on metallic surfaces and offer valuable insights into optimizing biocompatibility and controlling cellular responses,which may pave the way for innovative advances in vascular care and contribute to the ongoing improvement of stent insertion.
基金supported by a Korean National Research Foundation(NRF)grant funded by the Korea government(MSIT)(No.2017M3A9G1027929)supported by both the National Research Council of Science&Technology 424(NST)grant by the Korean government(MSIP)(CAP-17-01-KIST Europe)KIST project(2E31121).
文摘Developing a universal culture platform that manipulates cell fate is one of the most important tasks in the investigation of the role of the cellular microenvironment.This study focuses on the application of topographical and electrical field stimuli to human myogenic precursor cell(hMPC)cultures to assess the influences of the adherent direction,proliferation,and differentiation,and induce preconditioning-induced therapeutic benefits.First,a topographical surface of commercially available culture dishes was achieved by femtosecond laser texturing.The detachable biphasic electrical current system was then applied to the hMPCs cultured on laser-textured culture dishes.Laser-textured topographies were remarkably effective in inducing the assembly of hMPC myotubes by enhancing the orientation of adherent hMPCs compared with flat surfaces.Furthermore,electrical field stimulation through laser-textured topographies was found to promote the expression of myogenic regulatory factors compared with nonstimulated cells.As such,we successfully demonstrated that the combined stimulation of topographical and electrical cues could effectively enhance the myogenic maturation of hMPCs in a surface spatial and electrical field-dependent manner,thus providing the basis for therapeutic strategies.
