AIM: To investigate the putative role of human papillomavirus (HPV) infection in the carcinogenesis of esophageal squamous cell carcinoma in China.METHODS: Twenty-three esophageal squamous cell carcinoma samples and t...AIM: To investigate the putative role of human papillomavirus (HPV) infection in the carcinogenesis of esophageal squamous cell carcinoma in China.METHODS: Twenty-three esophageal squamous cell carcinoma samples and the distal normal epithelium from Shanxi Province, and 25 more esophageal squamous cell carcinoma samples from Anyang city, two areas with a high incidence of esophageal cancer in China, were detected for the existence of HPV-16 DNA by PCR, mRNA in situ hybridization (ISH) and immunohistochemistry (IHC) targeting HPV-16 E6 gene.
RESULTS: There were approximately 64 % (31/48) patients having HPV-16 DNA in tumor samples, among them nearly twothirds (19/31) samples were detected with mRNA expression of HPV-16 E6. However, in the normal esophageal epithelium from cancer patients, the DNA and mRNA of HPV-16 were found with much less rate: 34.7 % (8/23) and 26.1% (6/23) respectively.In addition, at protein level detected by IHC assay, 27.1% (13/48) tumor samples had virus oncoprotein E6 expression, while only one case of normal epithelium was found positive.CONCLUSION: HPV infection, especially type 16, should be considered as a risk factor for esophageal malignancies in China.展开更多
AIM: To investigate the expression of 1A6 gene in the lesionsduring the development of intestinal gastric carcinoma.METHODS: One hundred and thirty-six cases of intestinalmetaplasia (IM) from surgical resections and b...AIM: To investigate the expression of 1A6 gene in the lesionsduring the development of intestinal gastric carcinoma.METHODS: One hundred and thirty-six cases of intestinalmetaplasia (IM) from surgical resections and biopsy wereclassified by mucous staining. Expression of 1A6 in all caseswas detected using immunohistochemical S-P method.RESULTS: The positive rates of 1A6in normal and superficialgastritis (SG), severe atrophic gastritis (SAG), type Ⅰ, Ⅱ, ⅢIM, dysplasia (Dys) and intestinal gastric carcinoma (IGC)were 12.2 %, 16.7 %, 7.1%, 22.6 %, 47.8 %, 46.9 % and60.8 %, respectively. A significant difference among typeⅢ IM and SG, SAG, type I and Ⅱ IM was found (P＜0.01),the difference between type Ⅲ and Dys, IGC being notsignificant.CONCLUSION: As a new tumor-related gene, expression of1A6 may be an effective parameter to predict the malignanttransformation of precancerous lesion to gastric carcinoma.展开更多
AIM: To verify the effectiveness of denaturing highperformance liquid chromatography (DHPLC) in detecting somatic mutation of p53 gene in gastric carcinoma tissues.The superiority of this method has been proved in the...AIM: To verify the effectiveness of denaturing highperformance liquid chromatography (DHPLC) in detecting somatic mutation of p53 gene in gastric carcinoma tissues.The superiority of this method has been proved in the detection of germline mutations, but it was not very affirmative with respect to somatic mutations in tumor specimens. ST7 gene, a candidate tumor suppressor gene identified recently at human chromosome 7q31.1, was also detected because LOH at this site has also been widely reported in stomach cancer.METHODS: DNA was extracted from 39 cases of surgical gastric carcinoma specimen and their correspondent normal mucosa. Seven fragments spanning the 11 exons were used to detect the mutation of p53 gene and the four exons reported to have mutations in ST7 gene were amplified by PCR and directly analyzed by DHPLC without mixing with wild-type allele.RESULTS: In the analysis of p53 gene mutation, 9 aberrant DHPLC chromatographies were found in tumor tissues, while their normal-adjacent counterparts running in parallel showed a normal shape. Subsequent sequencing revealed nine sequence variations, 1 polymorphism and 8 mutations including 3 mutations not reported before. The mutation rate of p53 gene (21%) was consistent with that previously reported. Furthermore, no additional aberrant chromatography was found when wild-type DNA was added into the DNA of other 30 tumor samples that showed normal shapes previously. The positivity of p53 mutations was significantly higher in intestinal-type carcinomas (40 %) than that in diffuse-type (8.33 %) carcinomas of the stomach. No mutation of ST7 gene was found.CONCLUSION: DHPLC is a very convenient method for the detection of somatic mutations in gastric carcinoma. The amount of wild type alleles supplied by the non-tumorous cells in gastric tumor specimens is enough to form heteroduplex with mutant alleles for DHPLC detection. ST7 gene may not be the target gene of inactivation at 7q31 site in gastric carcinoma.展开更多
Introgression of agronomically important traits from barley to wheat is important for the improvement of wheat. Also, knowledge about precise location of genes in barley chromosomes is a prerequisite for map based gen...Introgression of agronomically important traits from barley to wheat is important for the improvement of wheat. Also, knowledge about precise location of genes in barley chromosomes is a prerequisite for map based gene isolation. The isolated genes would be introduced into wheat by crop transformation and consequently improve wheat. Many attempts have been conducted to achieve chromosome mediated introgressions from barley to wheat and to construct chromosome maps of barley showing physical locations of genes in the past. In the present study I have developed an alternative chromosome mutation inducing system capable of producing barley/wheat translocations and deletions in barley chromosome. The following is the summary. 1)In the attempt to induce breakage in barley chromosomes, I introduced a gametocidal chromosome 2C into six wheat barley addition lines. Chromosome 2C, from Aegilops cylindrica, which is a related species of wheat, has a gametocidal action causing chromosome breakage in the progeny of the monosomic 2C addition line of Chinese Spring wheat. The critical plants (21″+H″+2C′), disomic for each of barley chromosomes and monosomic for the 2C chromosome, were obtained. 2)The six critical lines were either selfed or backcrossed with the respective wheat barley addition lines. The selfed and backcrossed progeny of these lines were cytologically investigated by N banding and FISH using the barley probe HvT01 that is specific to the subterminal repeats of barley chromosomes. Various types of structural aberrations, most of which were deletions and translocations, were detected for all barley chromosomes with frequencies ranging from 10.8% to 27.9%. 3)Chromosome 7H was chosen to investigate the distribution of the breakpoints in the aberrations. Reciprocal crosses were made between the mutation inducing common wheat line (or critical lines) (21″+7H″+2C′) and the 7H addition line of common wheat (21″+7H″) to obtain more 7H deletions and 7H/wheat translocations. There were various types of aberrations as observed in the previous study. The breakpoints of these deletions and translocations appeared to distribute along the entire length of chromosome 7H.展开更多
A F2 mapping population was developed by crossing a Chinese cabbage-pe-tsai variety CC156 and an oil type Rapid cycling RC144 which were different from each other in morphology, maturity, self-compatibility, plant hei...A F2 mapping population was developed by crossing a Chinese cabbage-pe-tsai variety CC156 and an oil type Rapid cycling RC144 which were different from each other in morphology, maturity, self-compatibility, plant height, etc. Using 244 AFLP markers a map was constructed containing 10 main linkage groups covering a total distance of 857 cM, corresponding to 3.5 cM per marker. Length of linkage groups varied from 43 to 125 cM and the number of AFLP markers linkage to each group ranged from 7 to 41.展开更多
基金grants from the National Natural Science Foundation of China,No.39925020State Key Basic Research Program,No.G 1998051204
文摘AIM: To investigate the putative role of human papillomavirus (HPV) infection in the carcinogenesis of esophageal squamous cell carcinoma in China.METHODS: Twenty-three esophageal squamous cell carcinoma samples and the distal normal epithelium from Shanxi Province, and 25 more esophageal squamous cell carcinoma samples from Anyang city, two areas with a high incidence of esophageal cancer in China, were detected for the existence of HPV-16 DNA by PCR, mRNA in situ hybridization (ISH) and immunohistochemistry (IHC) targeting HPV-16 E6 gene.
RESULTS: There were approximately 64 % (31/48) patients having HPV-16 DNA in tumor samples, among them nearly twothirds (19/31) samples were detected with mRNA expression of HPV-16 E6. However, in the normal esophageal epithelium from cancer patients, the DNA and mRNA of HPV-16 were found with much less rate: 34.7 % (8/23) and 26.1% (6/23) respectively.In addition, at protein level detected by IHC assay, 27.1% (13/48) tumor samples had virus oncoprotein E6 expression, while only one case of normal epithelium was found positive.CONCLUSION: HPV infection, especially type 16, should be considered as a risk factor for esophageal malignancies in China.
基金National Key Fundamental Research 973 Project, No 1998051203National High Technology Research and Development Program of China(863 Program),No2001AA221121
文摘AIM: To investigate the expression of 1A6 gene in the lesionsduring the development of intestinal gastric carcinoma.METHODS: One hundred and thirty-six cases of intestinalmetaplasia (IM) from surgical resections and biopsy wereclassified by mucous staining. Expression of 1A6 in all caseswas detected using immunohistochemical S-P method.RESULTS: The positive rates of 1A6in normal and superficialgastritis (SG), severe atrophic gastritis (SAG), type Ⅰ, Ⅱ, ⅢIM, dysplasia (Dys) and intestinal gastric carcinoma (IGC)were 12.2 %, 16.7 %, 7.1%, 22.6 %, 47.8 %, 46.9 % and60.8 %, respectively. A significant difference among typeⅢ IM and SG, SAG, type I and Ⅱ IM was found (P＜0.01),the difference between type Ⅲ and Dys, IGC being notsignificant.CONCLUSION: As a new tumor-related gene, expression of1A6 may be an effective parameter to predict the malignanttransformation of precancerous lesion to gastric carcinoma.
基金National Science Fund for Distinguished Young Scholars,No.30125017the Major State Basic Research Development Program of China(973 Program),No.G1998051203
文摘AIM: To verify the effectiveness of denaturing highperformance liquid chromatography (DHPLC) in detecting somatic mutation of p53 gene in gastric carcinoma tissues.The superiority of this method has been proved in the detection of germline mutations, but it was not very affirmative with respect to somatic mutations in tumor specimens. ST7 gene, a candidate tumor suppressor gene identified recently at human chromosome 7q31.1, was also detected because LOH at this site has also been widely reported in stomach cancer.METHODS: DNA was extracted from 39 cases of surgical gastric carcinoma specimen and their correspondent normal mucosa. Seven fragments spanning the 11 exons were used to detect the mutation of p53 gene and the four exons reported to have mutations in ST7 gene were amplified by PCR and directly analyzed by DHPLC without mixing with wild-type allele.RESULTS: In the analysis of p53 gene mutation, 9 aberrant DHPLC chromatographies were found in tumor tissues, while their normal-adjacent counterparts running in parallel showed a normal shape. Subsequent sequencing revealed nine sequence variations, 1 polymorphism and 8 mutations including 3 mutations not reported before. The mutation rate of p53 gene (21%) was consistent with that previously reported. Furthermore, no additional aberrant chromatography was found when wild-type DNA was added into the DNA of other 30 tumor samples that showed normal shapes previously. The positivity of p53 mutations was significantly higher in intestinal-type carcinomas (40 %) than that in diffuse-type (8.33 %) carcinomas of the stomach. No mutation of ST7 gene was found.CONCLUSION: DHPLC is a very convenient method for the detection of somatic mutations in gastric carcinoma. The amount of wild type alleles supplied by the non-tumorous cells in gastric tumor specimens is enough to form heteroduplex with mutant alleles for DHPLC detection. ST7 gene may not be the target gene of inactivation at 7q31 site in gastric carcinoma.
文摘Introgression of agronomically important traits from barley to wheat is important for the improvement of wheat. Also, knowledge about precise location of genes in barley chromosomes is a prerequisite for map based gene isolation. The isolated genes would be introduced into wheat by crop transformation and consequently improve wheat. Many attempts have been conducted to achieve chromosome mediated introgressions from barley to wheat and to construct chromosome maps of barley showing physical locations of genes in the past. In the present study I have developed an alternative chromosome mutation inducing system capable of producing barley/wheat translocations and deletions in barley chromosome. The following is the summary. 1)In the attempt to induce breakage in barley chromosomes, I introduced a gametocidal chromosome 2C into six wheat barley addition lines. Chromosome 2C, from Aegilops cylindrica, which is a related species of wheat, has a gametocidal action causing chromosome breakage in the progeny of the monosomic 2C addition line of Chinese Spring wheat. The critical plants (21″+H″+2C′), disomic for each of barley chromosomes and monosomic for the 2C chromosome, were obtained. 2)The six critical lines were either selfed or backcrossed with the respective wheat barley addition lines. The selfed and backcrossed progeny of these lines were cytologically investigated by N banding and FISH using the barley probe HvT01 that is specific to the subterminal repeats of barley chromosomes. Various types of structural aberrations, most of which were deletions and translocations, were detected for all barley chromosomes with frequencies ranging from 10.8% to 27.9%. 3)Chromosome 7H was chosen to investigate the distribution of the breakpoints in the aberrations. Reciprocal crosses were made between the mutation inducing common wheat line (or critical lines) (21″+7H″+2C′) and the 7H addition line of common wheat (21″+7H″) to obtain more 7H deletions and 7H/wheat translocations. There were various types of aberrations as observed in the previous study. The breakpoints of these deletions and translocations appeared to distribute along the entire length of chromosome 7H.
基金This study was sponsored by Sino-Dutch Genomic Project of Horticultural Crops(2001CB711102)National 863 Program of China(2003AA207120).
文摘A F2 mapping population was developed by crossing a Chinese cabbage-pe-tsai variety CC156 and an oil type Rapid cycling RC144 which were different from each other in morphology, maturity, self-compatibility, plant height, etc. Using 244 AFLP markers a map was constructed containing 10 main linkage groups covering a total distance of 857 cM, corresponding to 3.5 cM per marker. Length of linkage groups varied from 43 to 125 cM and the number of AFLP markers linkage to each group ranged from 7 to 41.
