AIM: To analyze the expression levels of soluble form of CD95, CD95 ligand (sCD95 and SCD95L, respectively) in plasma and CD95 expression on CD3+cells in liver-transplanted recipients with acute rejection (AR). METHOD...AIM: To analyze the expression levels of soluble form of CD95, CD95 ligand (sCD95 and SCD95L, respectively) in plasma and CD95 expression on CD3+cells in liver-transplanted recipients with acute rejection (AR). METHODS: Peripheral blood mohonuclear cells (PBMCs) were isolated from 30 clinically liver transplanted recipients. CD95 expression on CD3+ cells was quantitatively measured by two-color fluorescence activated cell sorter (FACS) analysis. Lymphocyte surface phenotypes of CD4, CD8, CD16 and CD56 were determined by flow cytometry. Plasma levels of sCD95 and SCD95L were detected by Enzyme Linked-Immuno-Sorbent Assay (ELISA). The results were compared with that from normal healthy volunteers (n=15 individuals). RESULTS: FACS analysis showed that CD95 expression on CD3+ T cells was significantly increased in liver transplanted recipients with AR compared to that in stable recipients without rejection and infection or healthy individuals who did not undergo transplantation (18 676.93±11 588.34/molecule, 6 848.20±1 712.96/molecule, 6 418.01±2 001.95/molecule, respectively, P<0.01). Whereas no significant difference was seen between liver-transplanted stable recipients and healthy individuals. Furthermore, no significant differences were detected between each group with CD4/CD8 ratio or the percentage of CD16+56+cells. Plasma levels of sCD95 were significantly higher in transplanted recipients with AR compared to that in stable recipients or healthy individuals (391.88±196.00, 201.37±30.30, 148.83±58.25 pg/mL, respectively, P<0.01). In contrast, the plasma levels of sCD95L in liver-transplanted recipients were not significantly different from that in healthy individuals. CONCLUSION: The present results indicate that the increased CD95 expression on CD3+cells and the increased levels of sCD95 in plasma may modify the immunological situation of the recipients after transplantation or represent the ongoing graft rejection.展开更多
The corrected Stefan-Boltzmann law of black holes in the frame of a generalized uncertainty principle is derived through the Planck equation of thermal radiation. The result is different from the fiat spacetime： a co...The corrected Stefan-Boltzmann law of black holes in the frame of a generalized uncertainty principle is derived through the Planck equation of thermal radiation. The result is different from the fiat spacetime： a corrected T term induced by the generalized uncertainty principle emerges; the coefficient of the T4 term is no longer a constant while related with the spacetime in the vicinity of the event horizon and the thin film model. Applying this corrected law to black hole radiation, the highest temperature in the final time of the radiation and the corresponding remnant with a mass of order of Planck mass are obtained. The lifespan of black holes is also corrected, however, the correction is extremely small.展开更多
b Laboratory Center, Fuzhou University, Fuzhou, Fujian 350002, China Three palladium(II) complexes with the isonitrosobenzoylacetoneimine (HIBI) ligand, Pd( p CH 3C 6H 4IBI) 2(1), Pd(C 6H 5IBI) 2(2) and ...b Laboratory Center, Fuzhou University, Fuzhou, Fujian 350002, China Three palladium(II) complexes with the isonitrosobenzoylacetoneimine (HIBI) ligand, Pd( p CH 3C 6H 4IBI) 2(1), Pd(C 6H 5IBI) 2(2) and Pd 2Cl 2(C 6H 5CH 2IBI) 2·CHCl 3(3), were prepared and characterized by IR, Raman and X ray diffraction studies. The geometries around the palladium atoms in the complexes 1 and 2 are distorted trans PdN 4 square planes, and the Schiff base ligands RIBI - are coordinated through their oximo nitrogen atoms and imino nitrogen atoms. The week Pd...H-C agostic interactions [Pd...H=0.2764 nm] complete the hexacoordinate environment around palladium in the complex 1. The octahedral deformation of the classical square planar environment of the Pd atom is due to the week Pd...O(1b) interactions [Pd-O(1b)=0.3157(9) nm] in the complex 2. The complex 3 is a first example of binuclear complex with isonitrosoketoimine ligands, in which one of oximo groups is coordinated through oximo nitrogen and oximo oxygen atoms.展开更多
文摘AIM: To analyze the expression levels of soluble form of CD95, CD95 ligand (sCD95 and SCD95L, respectively) in plasma and CD95 expression on CD3+cells in liver-transplanted recipients with acute rejection (AR). METHODS: Peripheral blood mohonuclear cells (PBMCs) were isolated from 30 clinically liver transplanted recipients. CD95 expression on CD3+ cells was quantitatively measured by two-color fluorescence activated cell sorter (FACS) analysis. Lymphocyte surface phenotypes of CD4, CD8, CD16 and CD56 were determined by flow cytometry. Plasma levels of sCD95 and SCD95L were detected by Enzyme Linked-Immuno-Sorbent Assay (ELISA). The results were compared with that from normal healthy volunteers (n=15 individuals). RESULTS: FACS analysis showed that CD95 expression on CD3+ T cells was significantly increased in liver transplanted recipients with AR compared to that in stable recipients without rejection and infection or healthy individuals who did not undergo transplantation (18 676.93±11 588.34/molecule, 6 848.20±1 712.96/molecule, 6 418.01±2 001.95/molecule, respectively, P<0.01). Whereas no significant difference was seen between liver-transplanted stable recipients and healthy individuals. Furthermore, no significant differences were detected between each group with CD4/CD8 ratio or the percentage of CD16+56+cells. Plasma levels of sCD95 were significantly higher in transplanted recipients with AR compared to that in stable recipients or healthy individuals (391.88±196.00, 201.37±30.30, 148.83±58.25 pg/mL, respectively, P<0.01). In contrast, the plasma levels of sCD95L in liver-transplanted recipients were not significantly different from that in healthy individuals. CONCLUSION: The present results indicate that the increased CD95 expression on CD3+cells and the increased levels of sCD95 in plasma may modify the immunological situation of the recipients after transplantation or represent the ongoing graft rejection.
基金Supported by the National Natural Science Foundation of China under Grant Nos 11273009 and 11303006.
文摘The corrected Stefan-Boltzmann law of black holes in the frame of a generalized uncertainty principle is derived through the Planck equation of thermal radiation. The result is different from the fiat spacetime： a corrected T term induced by the generalized uncertainty principle emerges; the coefficient of the T4 term is no longer a constant while related with the spacetime in the vicinity of the event horizon and the thin film model. Applying this corrected law to black hole radiation, the highest temperature in the final time of the radiation and the corresponding remnant with a mass of order of Planck mass are obtained. The lifespan of black holes is also corrected, however, the correction is extremely small.
基金theEducationCommissionofZhejiangProvince (No .2 0 0 0 0 16 8)
文摘b Laboratory Center, Fuzhou University, Fuzhou, Fujian 350002, China Three palladium(II) complexes with the isonitrosobenzoylacetoneimine (HIBI) ligand, Pd( p CH 3C 6H 4IBI) 2(1), Pd(C 6H 5IBI) 2(2) and Pd 2Cl 2(C 6H 5CH 2IBI) 2·CHCl 3(3), were prepared and characterized by IR, Raman and X ray diffraction studies. The geometries around the palladium atoms in the complexes 1 and 2 are distorted trans PdN 4 square planes, and the Schiff base ligands RIBI - are coordinated through their oximo nitrogen atoms and imino nitrogen atoms. The week Pd...H-C agostic interactions [Pd...H=0.2764 nm] complete the hexacoordinate environment around palladium in the complex 1. The octahedral deformation of the classical square planar environment of the Pd atom is due to the week Pd...O(1b) interactions [Pd-O(1b)=0.3157(9) nm] in the complex 2. The complex 3 is a first example of binuclear complex with isonitrosoketoimine ligands, in which one of oximo groups is coordinated through oximo nitrogen and oximo oxygen atoms.
