AIM:To investigate the frequency of genomic in stability inmurine hepatocellular carcinoma (HCC) cell lines Hca/A2-P (P) and Hca/163-F(F) with low and high metastatic capacity,and to explore its association with the o...AIM:To investigate the frequency of genomic in stability inmurine hepatocellular carcinoma (HCC) cell lines Hca/A2-P (P) and Hca/163-F(F) with low and high metastatic capacity,and to explore its association with the occurrence and metastasis of hepatocellular carcinomas.METHODS:Forty microsatellite markers were randomly selected to examine P and F cells for genomic instability using PCR-simple sequence length polymorphism (PCR-SSLP) analysis.RESULTS:Allelic genes on the chromosomes of P cell line with thirty informative microsatellite loci were paralleled to those of inbred strain C3H mouse, while those of F cell line with 28 loci were paralleled to those of inbred strain C3H mice. The frequency of microsatellite alterations was 37.5% and 42.5% in P cell line and F cell line, respectively. There were different alterations of allelic band 9 at loci between P and F cells, among which, the frequency of microsatellite alterations was most commonly seen on chromosomes 3,7,11 and 16.CONCLUSION:Genomic instability in mouse chromosomes 3, 7, 11 and 16 may play a more important role in the development and progression of HCC in mice. It is suggested that these two sub-clones derived from a same hepatic tumor in homozygous mouse present different genetic features.展开更多
Interleukin 12(IL-12)and/or interleukin 18(IL-18)gene ablated mice were applied for the investigation of the tissue expression of interferon γ(IFN-γ).For IL-12^(-/-),IL-18^(-/-),IL-12^(-/-)/18^(-/-) and wt mice,repr...Interleukin 12(IL-12)and/or interleukin 18(IL-18)gene ablated mice were applied for the investigation of the tissue expression of interferon γ(IFN-γ).For IL-12^(-/-),IL-18^(-/-),IL-12^(-/-)/18^(-/-) and wt mice,reproductive performance were recorded and IFN-γ concentrations in heart,lung,liver,spleen,kidney and serum were quantified by ELISA. There were no significant differences of IFN-γ in heart,lung and kidney between 4 strains although control group was higher.It was observed that for IL-12^(-/-) mice,compared with other 3 groups,IFN-γ in liver and spleen were decreased(p<0.05)and reproductive performance appeared to be impaired.Serum IFN-γ level of IL-12^(-/-)/18^(-/-) mice was significantly higher(p<0.05).It was showed that IFN-γ productions under the normal condition were independent upon IL-12 and IL-18,its expressions in various tissues were different,and optimal IFN-γ is necessary for the normal growth and development of mammals.This study is helpful for clinical cytokines therapy.Cellular & Molecular Immunology.2005;2(1):68-72.展开更多
基金Supported by the National Natural Science Foundation of China,No.39900173the Major State Key Basic Research Development Program of China,No.G20000161061
文摘AIM:To investigate the frequency of genomic in stability inmurine hepatocellular carcinoma (HCC) cell lines Hca/A2-P (P) and Hca/163-F(F) with low and high metastatic capacity,and to explore its association with the occurrence and metastasis of hepatocellular carcinomas.METHODS:Forty microsatellite markers were randomly selected to examine P and F cells for genomic instability using PCR-simple sequence length polymorphism (PCR-SSLP) analysis.RESULTS:Allelic genes on the chromosomes of P cell line with thirty informative microsatellite loci were paralleled to those of inbred strain C3H mouse, while those of F cell line with 28 loci were paralleled to those of inbred strain C3H mice. The frequency of microsatellite alterations was 37.5% and 42.5% in P cell line and F cell line, respectively. There were different alterations of allelic band 9 at loci between P and F cells, among which, the frequency of microsatellite alterations was most commonly seen on chromosomes 3,7,11 and 16.CONCLUSION:Genomic instability in mouse chromosomes 3, 7, 11 and 16 may play a more important role in the development and progression of HCC in mice. It is suggested that these two sub-clones derived from a same hepatic tumor in homozygous mouse present different genetic features.
文摘Interleukin 12(IL-12)and/or interleukin 18(IL-18)gene ablated mice were applied for the investigation of the tissue expression of interferon γ(IFN-γ).For IL-12^(-/-),IL-18^(-/-),IL-12^(-/-)/18^(-/-) and wt mice,reproductive performance were recorded and IFN-γ concentrations in heart,lung,liver,spleen,kidney and serum were quantified by ELISA. There were no significant differences of IFN-γ in heart,lung and kidney between 4 strains although control group was higher.It was observed that for IL-12^(-/-) mice,compared with other 3 groups,IFN-γ in liver and spleen were decreased(p<0.05)and reproductive performance appeared to be impaired.Serum IFN-γ level of IL-12^(-/-)/18^(-/-) mice was significantly higher(p<0.05).It was showed that IFN-γ productions under the normal condition were independent upon IL-12 and IL-18,its expressions in various tissues were different,and optimal IFN-γ is necessary for the normal growth and development of mammals.This study is helpful for clinical cytokines therapy.Cellular & Molecular Immunology.2005;2(1):68-72.
