【Objectives】Si and microbial application could relieve the crop replanting problems(CRPs).We further studied the change of key microorganisms that are related to the beneficial effects,aiming at provide reference fo...【Objectives】Si and microbial application could relieve the crop replanting problems(CRPs).We further studied the change of key microorganisms that are related to the beneficial effects,aiming at provide reference for the manufacture and application of both microbial agents and Si fertilizer in food lily production.【Methods】A field experiment was conducted over a three-year period,from March 2019 to March 2022.The experimental field had been continuously cultivated with lily for 9 years.Three treatments were established:silicon fertilizer(SF),microbial agents(“Special 8^(TM)”,MF),and combined application of silicon fertilizer and microbial agents(SMF).A control group with blank soil(CK)was also included.At seedling stage of Lanzhou lilies in 2020 and 2021,the shoot and bulb dry weight,and the plant height and stem diameter of Lanzhou lilies were investigated for calculation of seedling index.In July 2020,20 plants were selected in each plot,and root zone soils were sampled at a depth of 20 cm,10 cm away from the roots,and then mixed to form a composite sample.The soil available Si and organic matter content were analyzed,and the fungal community structure and some specific microbial groups in soils were determined with high-throughput sequencing of ITS.【Results】All the three treatments significantly enhanced the lily plant growth and the seedling index,compared to CK.Besides,SF and MF treatments increased the relative abundances(RA)and diversity of fungal communities,and altered the community structures.The RA of some specific groups were found to be significantly correlated with the seedling index and/or soil available Si.Of them,the RA of the genera Fusarium,Dactylonectria,Humicola,Stilbella,and the species Humicola_grisea showed a positive correlation,while that of the genera Mortierella,Stilbella,Holtermanniella,and the species Mortierella_fatshederae showed a negative correlation with seedling index.The genera Fusarium,Stilbella,the species Humicola_grisea,and Dactylonectria_estremocensis showed a positive correlation,while the genura Stilbella,and the species Mortierella fatshederae showed a negative correlation with available Si content.In the co-occurence network of top twenty fungal genera and top sixteen bacterial genera(RA>0.2%),Holtermanniella was the only genus that interacted with the bacteria and negatively correlated with bacterial genus Blastococcus.Holtermanniella was also the most densely connected genera,followed by the genus Fusarium,Didymella and Humicola.In addition,the genus Holtermanniella was the key species connecting fungal and bacterial community in soil.Fungal functional prediction revealed that SF,MF and SMF treatments decreased plant pathogens guilds and increased the beneficial guilds Ectomycorrhizal,plant saprophyte,leaf saprophyte,and arbuscular mycorrhizal compared to CK.【Conclusions】Combined application of silicon fertilizer and microbial agents can alleviate continuous replanting problems of Lanzhou lilies through restoring the fungal community diversity,and promoting plant residue depredation,thus reducing soil born disease incidence.The beneficial genus Humicola and its one species H.grisea acts as bioconversion,and the genus Acremonium acts as plant pathogen inhibitor.展开更多
Biochar application can alleviate the adverse effects of saline-alkali stress on crops.However,the long-term effects of one-off biochar application on soil physicochemical properties,salt concentration,nutrient availa...Biochar application can alleviate the adverse effects of saline-alkali stress on crops.However,the long-term effects of one-off biochar application on soil physicochemical properties,salt concentration,nutrient availability,soil enzyme activities,and rice yield under highly saline-alkali paddy soils remain unclear.Here,a 6-year paddy field study was conducted in a saline-alkali paddy field using two nitrogen application levels(0 and 225 kg ha−1)and four biochar application rates[0(T0),1.5%(T1.5),3.0%(T3.0),and 4.5%(T4.5)biochar,w/w].The results showed that compared with T0,the bulk density(BD)under T1.5,T3.0,and T4.5 treatments significantly decreased by 11.21%,16.33%,and 25.57%,while total porosity(Tp)and saturated hydraulic conductivity(Ks)increased by 19.15-27.34%and 3217.78-5539.83%,respectively.Biochar consistently improved soil macro-aggregates,mean weight diameter(MWD),and the percentage of water-stable aggregates(PWSA)over the years.Additionally,one-off application of biochar continuously reduced the soil Na+concentration,Na+/K+ratio,Na+/Ca2+ratio,saturated paste extract(ECe),exchangeable sodium percentage(ESP),and sodium adsorption ratio(SARe).However,it reduced the pH in 2021 and 2022 only.It enhanced the concentration of K+,Ca2+,Mg2+,and cation exchange capacity(CEC)over the 6-year study,indicating its longer-term positive impact.Furthermore,the one-off biochar application,especially under high application rate treatments(T3.0 and T4.5),significantly and continuously improved nutrient availability and soil enzyme activities.However,alkali-hydrolysable nitrogen(AN)decreased in the initial year of biochar application.The grain yield of T1.5,T3.0,and T4.5 surpassed that of T0 by 116.38%,141.24%,and 145.20%,respectively.Notably,the rice yield reached its peak with the treatment of 3.0%(w/w)in all 6 years of study period.These findings offered new perspectives on repairing and improving soil quality and production ability of highly saline-alkali paddy soils.展开更多
基金Key Research project of Gansu Province of China(22YF7NA108)National Natural Science Foundation of China(31860549)+1 种基金Industry Supporting Project from Education Department of Gansu Province(2023CYZC-49)Major Science and Technology project of Gansu province(24ZDNA006)。
文摘【Objectives】Si and microbial application could relieve the crop replanting problems(CRPs).We further studied the change of key microorganisms that are related to the beneficial effects,aiming at provide reference for the manufacture and application of both microbial agents and Si fertilizer in food lily production.【Methods】A field experiment was conducted over a three-year period,from March 2019 to March 2022.The experimental field had been continuously cultivated with lily for 9 years.Three treatments were established:silicon fertilizer(SF),microbial agents(“Special 8^(TM)”,MF),and combined application of silicon fertilizer and microbial agents(SMF).A control group with blank soil(CK)was also included.At seedling stage of Lanzhou lilies in 2020 and 2021,the shoot and bulb dry weight,and the plant height and stem diameter of Lanzhou lilies were investigated for calculation of seedling index.In July 2020,20 plants were selected in each plot,and root zone soils were sampled at a depth of 20 cm,10 cm away from the roots,and then mixed to form a composite sample.The soil available Si and organic matter content were analyzed,and the fungal community structure and some specific microbial groups in soils were determined with high-throughput sequencing of ITS.【Results】All the three treatments significantly enhanced the lily plant growth and the seedling index,compared to CK.Besides,SF and MF treatments increased the relative abundances(RA)and diversity of fungal communities,and altered the community structures.The RA of some specific groups were found to be significantly correlated with the seedling index and/or soil available Si.Of them,the RA of the genera Fusarium,Dactylonectria,Humicola,Stilbella,and the species Humicola_grisea showed a positive correlation,while that of the genera Mortierella,Stilbella,Holtermanniella,and the species Mortierella_fatshederae showed a negative correlation with seedling index.The genera Fusarium,Stilbella,the species Humicola_grisea,and Dactylonectria_estremocensis showed a positive correlation,while the genura Stilbella,and the species Mortierella fatshederae showed a negative correlation with available Si content.In the co-occurence network of top twenty fungal genera and top sixteen bacterial genera(RA>0.2%),Holtermanniella was the only genus that interacted with the bacteria and negatively correlated with bacterial genus Blastococcus.Holtermanniella was also the most densely connected genera,followed by the genus Fusarium,Didymella and Humicola.In addition,the genus Holtermanniella was the key species connecting fungal and bacterial community in soil.Fungal functional prediction revealed that SF,MF and SMF treatments decreased plant pathogens guilds and increased the beneficial guilds Ectomycorrhizal,plant saprophyte,leaf saprophyte,and arbuscular mycorrhizal compared to CK.【Conclusions】Combined application of silicon fertilizer and microbial agents can alleviate continuous replanting problems of Lanzhou lilies through restoring the fungal community diversity,and promoting plant residue depredation,thus reducing soil born disease incidence.The beneficial genus Humicola and its one species H.grisea acts as bioconversion,and the genus Acremonium acts as plant pathogen inhibitor.
基金funded by the National Natural Science Foundation of China(No.32071951)the Natural Science Foundation of Jilin Province(No.20230101258JC)the Chinese Scholarship Council(No.202008220050).
文摘Biochar application can alleviate the adverse effects of saline-alkali stress on crops.However,the long-term effects of one-off biochar application on soil physicochemical properties,salt concentration,nutrient availability,soil enzyme activities,and rice yield under highly saline-alkali paddy soils remain unclear.Here,a 6-year paddy field study was conducted in a saline-alkali paddy field using two nitrogen application levels(0 and 225 kg ha−1)and four biochar application rates[0(T0),1.5%(T1.5),3.0%(T3.0),and 4.5%(T4.5)biochar,w/w].The results showed that compared with T0,the bulk density(BD)under T1.5,T3.0,and T4.5 treatments significantly decreased by 11.21%,16.33%,and 25.57%,while total porosity(Tp)and saturated hydraulic conductivity(Ks)increased by 19.15-27.34%and 3217.78-5539.83%,respectively.Biochar consistently improved soil macro-aggregates,mean weight diameter(MWD),and the percentage of water-stable aggregates(PWSA)over the years.Additionally,one-off application of biochar continuously reduced the soil Na+concentration,Na+/K+ratio,Na+/Ca2+ratio,saturated paste extract(ECe),exchangeable sodium percentage(ESP),and sodium adsorption ratio(SARe).However,it reduced the pH in 2021 and 2022 only.It enhanced the concentration of K+,Ca2+,Mg2+,and cation exchange capacity(CEC)over the 6-year study,indicating its longer-term positive impact.Furthermore,the one-off biochar application,especially under high application rate treatments(T3.0 and T4.5),significantly and continuously improved nutrient availability and soil enzyme activities.However,alkali-hydrolysable nitrogen(AN)decreased in the initial year of biochar application.The grain yield of T1.5,T3.0,and T4.5 surpassed that of T0 by 116.38%,141.24%,and 145.20%,respectively.Notably,the rice yield reached its peak with the treatment of 3.0%(w/w)in all 6 years of study period.These findings offered new perspectives on repairing and improving soil quality and production ability of highly saline-alkali paddy soils.
