This study highlights the regulatory potential antibacterial and antiradical of Origanum elongatum essential oil(EO),an endemic medicinal plant of Morocco used for its various properties.The chemical composition of th...This study highlights the regulatory potential antibacterial and antiradical of Origanum elongatum essential oil(EO),an endemic medicinal plant of Morocco used for its various properties.The chemical composition of the EO was characterized using gas chromatography-mass spectrometry(GC-MS).The antibacterial activity against different agricultural phytopathogens was determined by disc diffusion and microatmosphere methods,as well as by the determination of minimum inhibitory concentrations(MIC)and minimum bactericidal concentration(MBC),while the antioxidant activity was evaluated by DPPH and FRAP assays.To complement the experimental analyses,a molecular docking approach was used to predict and elucidate the mechanisms of action of the identified bioactive com-pounds,both for their antioxidant and antibacterial properties.The GC-MS analysis revealed a chemical composition dominated by the major compounds:p-cymene-2-ol(25.31%),thymol(23.88%),andγ-terpinene(19.26%).Furthermore,antibacterial analyses performed using different methodological approaches(disc diffusion,microatmosphere,MIC,and MBC)showed significant inhibitory activity against all phytopathogens tested.Moreover,O.elongatum EO exhibited interesting antioxidant ability with an IC_(50) value of 168.251.14μg/mL for DPPH assay and EC_(50) value±of 164.221.04μg/mL for FRAP assay.Furthermore,in silico molecular docking demonstrated further insights into±the interactions between the oil’s active components and bacterial targets,supporting its mode of action.This in-depth characterization highlights the potential of O.elongatum EO as a natural alternative for the biocontrol of plant pathogens.It opens new perspectives for developing natural solutions to protect crops against plant diseases.展开更多
Essential oils(OEs),recognized for their antibacterial and antioxidant characteristics,have gained extensive application in the food sector as preservative agents.Moreover,mixtures of OEs may have more potent anti-bac...Essential oils(OEs),recognized for their antibacterial and antioxidant characteristics,have gained extensive application in the food sector as preservative agents.Moreover,mixtures of OEs may have more potent anti-bacterial and antioxidant effects than a single essential oil.Expanding on this foundation,the objective of this work was to highlight the combined antibacterial and antioxidant action of blending essential oils(EOs)derived from Moroccan Cladanthus mixtus(C.mixtus),Myrtus communis(M.communis),and Helichrysum italicum subsp.Italicum(H.italicum).Gas Chromatography-Mass Spectrometry(GC-MS)and Gas Chromatography-Flame Ioni-zation Detection(GC-FID)analyses have revealed that C.mixtus EO was marked byα-pinene(19.7%),santolina alcohol(11.53%)and germacrene D(10.59%),M.communis was characterized by 1,8-cineole(30.88%),α-pinene(25.65%),myrtenyl acetate(14.69%)and limonene(10.99%),while neryl acetate(16.27%),α-pinene(13.64%),β-selinene(8.79%),γ-curcumene(7,7%)were major compounds in H.italicum EO.C.mixtus showed the strongest efficacy against Escherichia coli(E.coli)and Staphylococcus aureus(S.aureus),while H.italicum demonstrated the strongest antioxidant effect.Subsequently,the methodology of mixture design(MDM)combined with artificial neural networks(ANNs)were used to improve the formulations of the EOs.The results obtained from the pre-dictive modeling revealed that the inhibition of E.coli and S.aureus was impacted by the ternary mixtures of C.mixtus,M.communis,and H.italicum,whereas the binary mixture of C.mixtus and H.italicum displayed the highest antioxidant activity.Statistical analysis indicated that both optimization approaches accurately predicted the biological activities.However,the accuracy of the ANNs models outperformed that of MDM.展开更多
文摘This study highlights the regulatory potential antibacterial and antiradical of Origanum elongatum essential oil(EO),an endemic medicinal plant of Morocco used for its various properties.The chemical composition of the EO was characterized using gas chromatography-mass spectrometry(GC-MS).The antibacterial activity against different agricultural phytopathogens was determined by disc diffusion and microatmosphere methods,as well as by the determination of minimum inhibitory concentrations(MIC)and minimum bactericidal concentration(MBC),while the antioxidant activity was evaluated by DPPH and FRAP assays.To complement the experimental analyses,a molecular docking approach was used to predict and elucidate the mechanisms of action of the identified bioactive com-pounds,both for their antioxidant and antibacterial properties.The GC-MS analysis revealed a chemical composition dominated by the major compounds:p-cymene-2-ol(25.31%),thymol(23.88%),andγ-terpinene(19.26%).Furthermore,antibacterial analyses performed using different methodological approaches(disc diffusion,microatmosphere,MIC,and MBC)showed significant inhibitory activity against all phytopathogens tested.Moreover,O.elongatum EO exhibited interesting antioxidant ability with an IC_(50) value of 168.251.14μg/mL for DPPH assay and EC_(50) value±of 164.221.04μg/mL for FRAP assay.Furthermore,in silico molecular docking demonstrated further insights into±the interactions between the oil’s active components and bacterial targets,supporting its mode of action.This in-depth characterization highlights the potential of O.elongatum EO as a natural alternative for the biocontrol of plant pathogens.It opens new perspectives for developing natural solutions to protect crops against plant diseases.
文摘Essential oils(OEs),recognized for their antibacterial and antioxidant characteristics,have gained extensive application in the food sector as preservative agents.Moreover,mixtures of OEs may have more potent anti-bacterial and antioxidant effects than a single essential oil.Expanding on this foundation,the objective of this work was to highlight the combined antibacterial and antioxidant action of blending essential oils(EOs)derived from Moroccan Cladanthus mixtus(C.mixtus),Myrtus communis(M.communis),and Helichrysum italicum subsp.Italicum(H.italicum).Gas Chromatography-Mass Spectrometry(GC-MS)and Gas Chromatography-Flame Ioni-zation Detection(GC-FID)analyses have revealed that C.mixtus EO was marked byα-pinene(19.7%),santolina alcohol(11.53%)and germacrene D(10.59%),M.communis was characterized by 1,8-cineole(30.88%),α-pinene(25.65%),myrtenyl acetate(14.69%)and limonene(10.99%),while neryl acetate(16.27%),α-pinene(13.64%),β-selinene(8.79%),γ-curcumene(7,7%)were major compounds in H.italicum EO.C.mixtus showed the strongest efficacy against Escherichia coli(E.coli)and Staphylococcus aureus(S.aureus),while H.italicum demonstrated the strongest antioxidant effect.Subsequently,the methodology of mixture design(MDM)combined with artificial neural networks(ANNs)were used to improve the formulations of the EOs.The results obtained from the pre-dictive modeling revealed that the inhibition of E.coli and S.aureus was impacted by the ternary mixtures of C.mixtus,M.communis,and H.italicum,whereas the binary mixture of C.mixtus and H.italicum displayed the highest antioxidant activity.Statistical analysis indicated that both optimization approaches accurately predicted the biological activities.However,the accuracy of the ANNs models outperformed that of MDM.
