Urban sediment generated by stormwater management techniques are highly contaminated with various trace elements. The characterization of trace element speciation and mobility are critical information to improve envir...Urban sediment generated by stormwater management techniques are highly contaminated with various trace elements. The characterization of trace element speciation and mobility are critical information to improve environmental risk assessment. This study investigates the spatial and temporal variability of major and trace element release from a sedimentary layer in Django Reinhart stormwater infiltration basin (Chassieu, eastern suburbs of Lyon, France). Sampling was conducted for 3 zones and two dates. Chemical characterization was performed (X-Ray diffraction, ICP-AES). The samples were submitted to Acid Neutralization Capacity & Base Neutralization Capacity ANC-BNC tests, according to European standard 14429 (AFNOR, 2015). Solid matrices were mixed with acid or basic solutions and physicochemical parameters and major and trace element release (i.e. Al, Ca, Fe, P, S, Si, Cu, Zn and total carbon) were followed as a function of pH. The results show that the urban sediment has no significant spatial and temporal variability with regards to element release. This observation is all the more surprising that the samples were collected in three contrasting zones regarding stormwater supply and hydric conditions. Element release follows the same trends as a function of pH with a bell-shaped solubilization curve exhibiting with the highest solubility at extreme pH values. However, the samples showed slight differences concerning the release of major elements. Such differences are related to slight differences in total mineral contents (organic matter, carbonates…) and chemical composition of the sediments. The results show that despite the varying environmental conditions, the sediment chemical properties can be considered as very stable and uniform over space, depending mainly on the local geochemical context and watershed characteristics. This study highlights the strength and affluence of the information obtained by ANC-BNC tests on the release of major and traces metal elements by urban sediments and brings relevant information regarding the management of these sediments.展开更多
The understanding of unsaturated flow in heterogeneous formations is a prerequisite to the understanding of pollutant transfer in the vadose zone and the proper management of infiltration basins settled over such hete...The understanding of unsaturated flow in heterogeneous formations is a prerequisite to the understanding of pollutant transfer in the vadose zone and the proper management of infiltration basins settled over such heterogeneous formations. This study addresses the effect of lithological heterogeneity of a glaciofluvial deposit on flow in the vadose zone underneath an infiltration basin settled in the Lyon suburbs. The basin had already been the subject of several previous studies, some of which demonstrated the impact of soil heterogeneity. But all of them were only based on the sedimentological study of a trench and no study addressed the potential spatial variability of results due to the spatial variability of soil heterogeneity. In this study, we model flow in the vadose zone for several case studies, including drainage, water infiltration during a rainfall event, and a complete meteorological chronic. These calculations were conducted for several sections, previously characterized in the basin using GPR and sedimentological study and compared with a blank (homogeneous section). The results clearly show that heterogeneity impacts unsaturated flow and that these impacts depend upon the section considered. Some geometrical architectural and textural parameters were proposed to explain the spatial variability and effect of the soil heterogeneity on unsaturated flow, thus establishing the first step towards modeling unsaturated flow in the basin at the meso-scale.展开更多
Due to its rapid movement, preferential flow (PF) in the vadose zone allows much faster contaminant transport, which may have a significant impact on ground-water quality. PF can occur in heterogeneous vadose zones an...Due to its rapid movement, preferential flow (PF) in the vadose zone allows much faster contaminant transport, which may have a significant impact on ground-water quality. PF can occur in heterogeneous vadose zones and it strongly depends on hydric and hydraulic conditions like entering flow rates at surface. This study deals with the modeling of the establishment of PF, and related solute transfer during the infiltration phase in a strongly heterogeneous glaciofluvial deposit. This deposit is made of four contrasting lithofacies (sand, gravel, bimodal gravel and matrix-free gravel) and lies underneath an urban infiltration basin (Lyon, France). Previous studies have been carried out on this site and linked the regionalization of soil pollution with the lithological heterogeneity. But none of them clearly demonstrated how heterogeneity could impact flow and solute transfer and may explain such a regionalization. In this study, we model flow and solute transfer at the trench scale for both uniform and heterogeneous profiles in order to characterize the effect of lithological heterogeneity. In addition, such a modeling was performed for two different entering flow rates to depict the influence of condition at surface on PF. A key result is that heterogeneity clearly impacts unsaturated flow and solute transfer. Numerical modeling permitted pointing out the existence of PF paths associated with the sedimentary heterogeneity of the glaciofluvial deposit. For lower surface fluxes, the sand lens and matrix-free gravel were the sources of capillary barrier effects, leading to a funneled flow and a groundwater recharge characterized by earlier and more dispersed wetting fronts. Such a flow pattern enhances solutes transfer and reduces solute retention by soil. Thus, the effect of heterogeneity on solute transfer is significant, especially for the most reactive solutes.展开更多
In recent years, many studies have been carried out on colloidal particle transfer in the unsaturated zone because they can be a risk to the environment either directly or as a vector of pollutants. A study was conduc...In recent years, many studies have been carried out on colloidal particle transfer in the unsaturated zone because they can be a risk to the environment either directly or as a vector of pollutants. A study was conducted on the influence of porous media structure in unsaturated conditions on colloidal particle transport. Three granular materials were set up in columns to replicate a fluvio-glacial soil from the unsaturated zone in the Lyon area (France). It is a sand, a bimodal mixture in equal proportion by weight of sand and gravel, and a fraction of bimodal mixture. Nanoparticles of silica (SiO2-Au-FluoNPs), having a hydrodynamic diameter between 50 and 60 nm, labeled by organic fluorescent molecules were used to simulate the transport of colloidal particles. A nonreactive tracer, bromide ion (Br-) at a concentration of C0,s = 10-2 M was used to determine the hydrodispersive properties of porous media. The tests were carried out first, with a solution of nanoparticles (C0,p = 0.2 g/L) and secondly, with a solution of nanoparticles and bromine. The transfer model based on fractionation of water into two phases, mobile and immobile, MIM, correctly fits the elution curves. The retention of colloidal particles is greater in the two media of bimodal particle size than that in the sand, which clearly demonstrates the role of textural heterogeneity in the retention mechanism. The increase in ionic strength produced by alimenting the columns with colloidal particle suspension in the presence of bromide, increases retention up to 25% in the sand. The total concentration profile of nanoparticles collected at the end of the experiment shows that the colloidal particles are retained primarily at the entrance of the columns. Hydrodispersive calculated parameters indicate that flow is more heterogeneous in bimodal media compared to sand.展开更多
Background:The present article questions the relative importance of local-and large-scale processes on the long-term dynamics of fire in the subalpine belt in the western Alps.The study is based on soil charcoal datin...Background:The present article questions the relative importance of local-and large-scale processes on the long-term dynamics of fire in the subalpine belt in the western Alps.The study is based on soil charcoal dating and identification,several study sites in contrasting environmental conditions,and sampling of soil charcoal along the elevation gradient of each site.Based on local differences in biomass combustion,we hypothesize that local-scale or landscape-scale processes have driven the fire history,while combustion homogeneity supports the hypothesis of the importance of large-scale or macro-ecological processes,especially climate.Results:Biomass burning during the Holocene resulted from the nesting effects of climate,land use,and altitude,but was little influenced by slope exposure(north versus south),soil(dryness,pH,depth),and vegetation.The mid-Holocene(6500–2700 cal BP)was an important period for climate-driven biomass burning in the subalpine ecosystems of the western Alps,while fires over the last 2500 years appear much more episodic,prompting us to speculate that human activity has played a vital role in their occurrence.Conclusion:Our working hypothesis that the strength of local drivers should offset the effects of regional climate is not validated.The homogeneity of the fire regime between sites thus underscores that climate was the main driver during the Holocene of the western Alps.Long-term subalpine fires are controlled by climate at the millennial scale.Local conditions matter for little in determining variability at the century scale.The mid-Holocene was a chief period for climatic biomass burning in the subalpine zone,while fires during the late Holocene appear much more episodic,suggesting that social drivers has exercised key function on their control.展开更多
In periurban zones, urban wet weather discharges have been recognized as the most significant vector of pollution in aquatic environments. The discharge of this water without treatment into the aquatic environment cou...In periurban zones, urban wet weather discharges have been recognized as the most significant vector of pollution in aquatic environments. The discharge of this water without treatment into the aquatic environment could present an ecotoxicological risk for biocenosis. The aim of the INVASION project is to assess the potential ecotoxicological impact of a combined sewer overflow (CSO) on a peri-urban stream. A comparative study between upstream and downstream areas of the CSO allowed observing significant effects of this overflow on the river. We studied three layers of stream: surface water, benthic layer and hyporheic layer. To characterize the potential ecotoxicological risk of water and sediments, we used a battery of 4 bioassays: Daphnia magna, Vibrio fischeri, Brachionus calyciflorus and Heterocypris incongruens. In parallel, we measured the physico-chemical parameters: ammonium (NH4+), chromium (Cr), copper (Cu) and lead (Pb). An ecological risk is greatest for the hyporheic zone in downstream river, particularly for the solid phase. These results corroborated with the physico-chemical data obtained.展开更多
Toxicity of wastewater treatment plant (WWTP) effluents is usually assessed with standardized bioassays, as e.g. the Fish Embryo Toxicity test (FET), but assessment of sub-lethal toxic effects requires to develop more...Toxicity of wastewater treatment plant (WWTP) effluents is usually assessed with standardized bioassays, as e.g. the Fish Embryo Toxicity test (FET), but assessment of sub-lethal toxic effects requires to develop more adapted tests. The present work aimed to add the recording of several sub-lethal endpoints in exposed zebrafish embryo-larval stages in order to increase the sensitivity of residual toxicity evaluation of WWTP effluents using a semi-quantitative method (iFET score). This approach was complemented with the genotoxicity assessment on the exposed embryos, a sub-acute hazard particularly relevant to evaluate a potential chronic toxicity risk of low and multi-contaminated environmental matrices. Additionally, endocrine activities of effluents were quantified using human reporter cell lines. This test battery was applied to the assessment of the residual toxicity of five biological treatment effluents, further treated or not using various ozonation treatments intended to improve pharmaceutical compounds removal. Acute toxicity towards zebrafish embryos was very low. However, iFET score approach proposed was able to reveal residual toxicity through the presence of developmental abnormalities in all samples tested. Additionally, a low residual genotoxicity was measured in embryos exposed to two of the WWTP effluents when all excepted one exhibited a residual endocrine activity potential in the ng/L range providing complementary information on the occurrence of endocrine active chemicals and their reduction by different processes. To sum up, such a simplified and ecologically relevant test battery was found sensitive enough to characterize and differentiate various residual effluent’s ecotoxicity at contaminant levels of environmental concern.展开更多
Species and nestmate recognition in social insects occurs mostly through cuticular hydrocarbons acting as chemical cues. These compounds generate a colony-specific odor profile depending on genetic and environmental f...Species and nestmate recognition in social insects occurs mostly through cuticular hydrocarbons acting as chemical cues. These compounds generate a colony-specific odor profile depending on genetic and environmental factors. Species and nestmate recognition results in specific behavioral responses, regulating the level of aggression toward other individuals during an interaction. Although species discrimination and recognition cues have been poorly studied in the context of interspecific hybridization, such systems offer an opportunity to further investigate the influence of heritable and environmental factors on recognition. We explored the strength of discrimination in a hybrid zone between two ant species—Tetramorium immigrans and T. caespitum—by comparing cuticular hydrocarbon profiles and measuring intra- and interspecific worker aggression in both areas of sympatry and areas of allopatry among species. Species cuticular hydrocarbon profiles were well-differentiated and interspecific aggression was high, revealing highly discriminating species recognition cues. Hybrids’ cuticular hydrocarbon profiles consisted of a mixture of the parental bouquets, but also exhibited hybrid-specific patterns. Behavioral assays showed that T. immigrans is as aggressive toward hybrids as toward heterospecifics. Finally, aggression between heterospecific workers was lower when interacting individuals came from areas of sympatry among species than from areas of allopatry. Taken as a whole, these findings paint a particularly complex picture of the recognition system in T. immigrans, T. caespitum, and their hybrids, and highlight that hybrid zones afford a still underexplored opportunity for investigating recognition mechanisms and discrimination between species.展开更多
文摘Urban sediment generated by stormwater management techniques are highly contaminated with various trace elements. The characterization of trace element speciation and mobility are critical information to improve environmental risk assessment. This study investigates the spatial and temporal variability of major and trace element release from a sedimentary layer in Django Reinhart stormwater infiltration basin (Chassieu, eastern suburbs of Lyon, France). Sampling was conducted for 3 zones and two dates. Chemical characterization was performed (X-Ray diffraction, ICP-AES). The samples were submitted to Acid Neutralization Capacity & Base Neutralization Capacity ANC-BNC tests, according to European standard 14429 (AFNOR, 2015). Solid matrices were mixed with acid or basic solutions and physicochemical parameters and major and trace element release (i.e. Al, Ca, Fe, P, S, Si, Cu, Zn and total carbon) were followed as a function of pH. The results show that the urban sediment has no significant spatial and temporal variability with regards to element release. This observation is all the more surprising that the samples were collected in three contrasting zones regarding stormwater supply and hydric conditions. Element release follows the same trends as a function of pH with a bell-shaped solubilization curve exhibiting with the highest solubility at extreme pH values. However, the samples showed slight differences concerning the release of major elements. Such differences are related to slight differences in total mineral contents (organic matter, carbonates…) and chemical composition of the sediments. The results show that despite the varying environmental conditions, the sediment chemical properties can be considered as very stable and uniform over space, depending mainly on the local geochemical context and watershed characteristics. This study highlights the strength and affluence of the information obtained by ANC-BNC tests on the release of major and traces metal elements by urban sediments and brings relevant information regarding the management of these sediments.
文摘The understanding of unsaturated flow in heterogeneous formations is a prerequisite to the understanding of pollutant transfer in the vadose zone and the proper management of infiltration basins settled over such heterogeneous formations. This study addresses the effect of lithological heterogeneity of a glaciofluvial deposit on flow in the vadose zone underneath an infiltration basin settled in the Lyon suburbs. The basin had already been the subject of several previous studies, some of which demonstrated the impact of soil heterogeneity. But all of them were only based on the sedimentological study of a trench and no study addressed the potential spatial variability of results due to the spatial variability of soil heterogeneity. In this study, we model flow in the vadose zone for several case studies, including drainage, water infiltration during a rainfall event, and a complete meteorological chronic. These calculations were conducted for several sections, previously characterized in the basin using GPR and sedimentological study and compared with a blank (homogeneous section). The results clearly show that heterogeneity impacts unsaturated flow and that these impacts depend upon the section considered. Some geometrical architectural and textural parameters were proposed to explain the spatial variability and effect of the soil heterogeneity on unsaturated flow, thus establishing the first step towards modeling unsaturated flow in the basin at the meso-scale.
文摘Due to its rapid movement, preferential flow (PF) in the vadose zone allows much faster contaminant transport, which may have a significant impact on ground-water quality. PF can occur in heterogeneous vadose zones and it strongly depends on hydric and hydraulic conditions like entering flow rates at surface. This study deals with the modeling of the establishment of PF, and related solute transfer during the infiltration phase in a strongly heterogeneous glaciofluvial deposit. This deposit is made of four contrasting lithofacies (sand, gravel, bimodal gravel and matrix-free gravel) and lies underneath an urban infiltration basin (Lyon, France). Previous studies have been carried out on this site and linked the regionalization of soil pollution with the lithological heterogeneity. But none of them clearly demonstrated how heterogeneity could impact flow and solute transfer and may explain such a regionalization. In this study, we model flow and solute transfer at the trench scale for both uniform and heterogeneous profiles in order to characterize the effect of lithological heterogeneity. In addition, such a modeling was performed for two different entering flow rates to depict the influence of condition at surface on PF. A key result is that heterogeneity clearly impacts unsaturated flow and solute transfer. Numerical modeling permitted pointing out the existence of PF paths associated with the sedimentary heterogeneity of the glaciofluvial deposit. For lower surface fluxes, the sand lens and matrix-free gravel were the sources of capillary barrier effects, leading to a funneled flow and a groundwater recharge characterized by earlier and more dispersed wetting fronts. Such a flow pattern enhances solutes transfer and reduces solute retention by soil. Thus, the effect of heterogeneity on solute transfer is significant, especially for the most reactive solutes.
文摘In recent years, many studies have been carried out on colloidal particle transfer in the unsaturated zone because they can be a risk to the environment either directly or as a vector of pollutants. A study was conducted on the influence of porous media structure in unsaturated conditions on colloidal particle transport. Three granular materials were set up in columns to replicate a fluvio-glacial soil from the unsaturated zone in the Lyon area (France). It is a sand, a bimodal mixture in equal proportion by weight of sand and gravel, and a fraction of bimodal mixture. Nanoparticles of silica (SiO2-Au-FluoNPs), having a hydrodynamic diameter between 50 and 60 nm, labeled by organic fluorescent molecules were used to simulate the transport of colloidal particles. A nonreactive tracer, bromide ion (Br-) at a concentration of C0,s = 10-2 M was used to determine the hydrodispersive properties of porous media. The tests were carried out first, with a solution of nanoparticles (C0,p = 0.2 g/L) and secondly, with a solution of nanoparticles and bromine. The transfer model based on fractionation of water into two phases, mobile and immobile, MIM, correctly fits the elution curves. The retention of colloidal particles is greater in the two media of bimodal particle size than that in the sand, which clearly demonstrates the role of textural heterogeneity in the retention mechanism. The increase in ionic strength produced by alimenting the columns with colloidal particle suspension in the presence of bromide, increases retention up to 25% in the sand. The total concentration profile of nanoparticles collected at the end of the experiment shows that the colloidal particles are retained primarily at the entrance of the columns. Hydrodispersive calculated parameters indicate that flow is more heterogeneous in bimodal media compared to sand.
基金Financial support was provided to C.C.through a French incentive action fund from the Institut National des Sciences de l’Univers(INSU ACI ECCO)by an ARTEMIS grant for datings(PALEOFIRE program,INSU-CNRS,France).
文摘Background:The present article questions the relative importance of local-and large-scale processes on the long-term dynamics of fire in the subalpine belt in the western Alps.The study is based on soil charcoal dating and identification,several study sites in contrasting environmental conditions,and sampling of soil charcoal along the elevation gradient of each site.Based on local differences in biomass combustion,we hypothesize that local-scale or landscape-scale processes have driven the fire history,while combustion homogeneity supports the hypothesis of the importance of large-scale or macro-ecological processes,especially climate.Results:Biomass burning during the Holocene resulted from the nesting effects of climate,land use,and altitude,but was little influenced by slope exposure(north versus south),soil(dryness,pH,depth),and vegetation.The mid-Holocene(6500–2700 cal BP)was an important period for climate-driven biomass burning in the subalpine ecosystems of the western Alps,while fires over the last 2500 years appear much more episodic,prompting us to speculate that human activity has played a vital role in their occurrence.Conclusion:Our working hypothesis that the strength of local drivers should offset the effects of regional climate is not validated.The homogeneity of the fire regime between sites thus underscores that climate was the main driver during the Holocene of the western Alps.Long-term subalpine fires are controlled by climate at the millennial scale.Local conditions matter for little in determining variability at the century scale.The mid-Holocene was a chief period for climatic biomass burning in the subalpine zone,while fires during the late Holocene appear much more episodic,suggesting that social drivers has exercised key function on their control.
文摘In periurban zones, urban wet weather discharges have been recognized as the most significant vector of pollution in aquatic environments. The discharge of this water without treatment into the aquatic environment could present an ecotoxicological risk for biocenosis. The aim of the INVASION project is to assess the potential ecotoxicological impact of a combined sewer overflow (CSO) on a peri-urban stream. A comparative study between upstream and downstream areas of the CSO allowed observing significant effects of this overflow on the river. We studied three layers of stream: surface water, benthic layer and hyporheic layer. To characterize the potential ecotoxicological risk of water and sediments, we used a battery of 4 bioassays: Daphnia magna, Vibrio fischeri, Brachionus calyciflorus and Heterocypris incongruens. In parallel, we measured the physico-chemical parameters: ammonium (NH4+), chromium (Cr), copper (Cu) and lead (Pb). An ecological risk is greatest for the hyporheic zone in downstream river, particularly for the solid phase. These results corroborated with the physico-chemical data obtained.
基金financially supported by the French Ministry of Economy,Industry and Digital within the framework of the Project TRIUMPH(TReatIng Urban Micropollutants and Pharmaceuticals in wastewaters)labelled and managed by the European Eureka Cluster ACQUEAU.Laurent Simon(LEHNA,UMR 5023)is deeply acknowledge for the total and dissolved organic carbon analysis.
文摘Toxicity of wastewater treatment plant (WWTP) effluents is usually assessed with standardized bioassays, as e.g. the Fish Embryo Toxicity test (FET), but assessment of sub-lethal toxic effects requires to develop more adapted tests. The present work aimed to add the recording of several sub-lethal endpoints in exposed zebrafish embryo-larval stages in order to increase the sensitivity of residual toxicity evaluation of WWTP effluents using a semi-quantitative method (iFET score). This approach was complemented with the genotoxicity assessment on the exposed embryos, a sub-acute hazard particularly relevant to evaluate a potential chronic toxicity risk of low and multi-contaminated environmental matrices. Additionally, endocrine activities of effluents were quantified using human reporter cell lines. This test battery was applied to the assessment of the residual toxicity of five biological treatment effluents, further treated or not using various ozonation treatments intended to improve pharmaceutical compounds removal. Acute toxicity towards zebrafish embryos was very low. However, iFET score approach proposed was able to reveal residual toxicity through the presence of developmental abnormalities in all samples tested. Additionally, a low residual genotoxicity was measured in embryos exposed to two of the WWTP effluents when all excepted one exhibited a residual endocrine activity potential in the ng/L range providing complementary information on the occurrence of endocrine active chemicals and their reduction by different processes. To sum up, such a simplified and ecologically relevant test battery was found sensitive enough to characterize and differentiate various residual effluent’s ecotoxicity at contaminant levels of environmental concern.
基金This work was supported by the Conseil Departemental de Flsere,the French National Research Agency(ANR)through the LABEX IMU(ANR-10-LABX-0088)of Universite de Lyonwithin the program“Investissements d’Avenir”(ANR-11-IDEX-0007).
文摘Species and nestmate recognition in social insects occurs mostly through cuticular hydrocarbons acting as chemical cues. These compounds generate a colony-specific odor profile depending on genetic and environmental factors. Species and nestmate recognition results in specific behavioral responses, regulating the level of aggression toward other individuals during an interaction. Although species discrimination and recognition cues have been poorly studied in the context of interspecific hybridization, such systems offer an opportunity to further investigate the influence of heritable and environmental factors on recognition. We explored the strength of discrimination in a hybrid zone between two ant species—Tetramorium immigrans and T. caespitum—by comparing cuticular hydrocarbon profiles and measuring intra- and interspecific worker aggression in both areas of sympatry and areas of allopatry among species. Species cuticular hydrocarbon profiles were well-differentiated and interspecific aggression was high, revealing highly discriminating species recognition cues. Hybrids’ cuticular hydrocarbon profiles consisted of a mixture of the parental bouquets, but also exhibited hybrid-specific patterns. Behavioral assays showed that T. immigrans is as aggressive toward hybrids as toward heterospecifics. Finally, aggression between heterospecific workers was lower when interacting individuals came from areas of sympatry among species than from areas of allopatry. Taken as a whole, these findings paint a particularly complex picture of the recognition system in T. immigrans, T. caespitum, and their hybrids, and highlight that hybrid zones afford a still underexplored opportunity for investigating recognition mechanisms and discrimination between species.
