Objective:To develop a cost-effective method to reduce the time consumption of elution in immunoprecipitation.Methods Two volumes(125μL for Group C and 100μL for Group T)of elution buffer were used to explore whethe...Objective:To develop a cost-effective method to reduce the time consumption of elution in immunoprecipitation.Methods Two volumes(125μL for Group C and 100μL for Group T)of elution buffer were used to explore whether smaller volume could save testing time.Result:Time consumption of elution in Group T was significantly shorter than that in Group C,while the efficiency of eluted m6A-containing fragments and the performance of m^(6)A-Seq as indicated by m6A peak distributions showed no difference between the two groups.Conclusion:A smaller volume of elution buffer was an economical way to reduce time consumption in immunoprecipitation.展开更多
Herbivore insects deploy salivary effectors to manipulate the defense of their host plants.However,it remains unclear whether small RNAs from insects can function as effectors in regulating plant-insect interactions.H...Herbivore insects deploy salivary effectors to manipulate the defense of their host plants.However,it remains unclear whether small RNAs from insects can function as effectors in regulating plant-insect interactions.Here,we report that a microRNA(miR29-b)found in the saliva of the phloem-feeding whitefly(Bemisia tabaci)can transfer into the host plant phloem during feeding and fine-tune the defense response of tobacco(Nicotiana tabacum)plants.We show that the salivary gland-enriched BtmiR29-b is produced by BtDicer 1 and released into tobacco cells via salivary exosomes.Once inside the plant cells,BtmiR29-b hijacks tobacco Argonaute 1 to silence the defense gene Bcl-2-associated athanogene 4(NtBAG4).In tobacco,NtBAG4 acts as the positive regulator of phytohormones salicylic acid(SA)and jasmonic acid(JA),enhancing plant defense against whitefly attacks.Interestingly,we also found that miR29-b acts as a salivary effector in another Hemipteran insect,the aphid Myzus persicae,which inhibits tobacco resistance by degrading NtBAG4.Moreover,miR29-b is highly conserved in Hemiptera and across other insect orders such as Coleoptera,Hymenoptera,Orthoptera,and Blattaria.Computational analysis suggests that miR29-b may also target the evolutionarily conserved BAG4 gene in other plant species.We further provide evidence showing BtmiR29-b-mediated BAG4 cleavage and defense suppression in tomato(Solanum lycopersicum).Taken together,our work reveals that a conserved miR29-b effector from insects fine-tunes plant SA-and JA-mediated defense by cross-kingdom silencing of the host plant BAG4 gene,providing new insight into the defense and counter-defense mechanisms between herbivores and their host plants.展开更多
Chemotherapy serves as the primary therapeutic approach for triple-negative breast cancer(TNBC),yet its efficacy remains unsatisfactory.This study was a single-arm,open-label,single-center clinical trial(NCT05447702)i...Chemotherapy serves as the primary therapeutic approach for triple-negative breast cancer(TNBC),yet its efficacy remains unsatisfactory.This study was a single-arm,open-label,single-center clinical trial(NCT05447702)involving patients with newly diagnosed stage Ⅱ-Ⅲ TNBC at West China Hospital.The treatment regimen consisted of camrelizumab(200 mg intravenously every 2 weeks,12 cycles),apatinib(250 mg orally daily),and alternating chemotherapy[nab-paclitaxel(d1,8,15 every 4 weeks)for 4 cycles and epirubicin plus cyclophosphamide(every 2 weeks)for 4 cycles].From June 2023 to April 2024,35 patients were enrolled,of whom 1 patient withdrew due to adverse reaction intolerance.At treatment completion,the total pathological complete response(tpCR,ypT0/is,ypN0)rate was 67.6%(23/34),and the breast pCR(ypT0/is)rate was 70.6%(24/34).The overall response rate following neoadjuvant treatment reached 94.1%(32/34).展开更多
文摘Objective:To develop a cost-effective method to reduce the time consumption of elution in immunoprecipitation.Methods Two volumes(125μL for Group C and 100μL for Group T)of elution buffer were used to explore whether smaller volume could save testing time.Result:Time consumption of elution in Group T was significantly shorter than that in Group C,while the efficiency of eluted m6A-containing fragments and the performance of m^(6)A-Seq as indicated by m6A peak distributions showed no difference between the two groups.Conclusion:A smaller volume of elution buffer was an economical way to reduce time consumption in immunoprecipitation.
基金supported by National Natural Science Foundation of China(31925033,32161143008)National Key Research and Development Program(2021YFC2600100).
文摘Herbivore insects deploy salivary effectors to manipulate the defense of their host plants.However,it remains unclear whether small RNAs from insects can function as effectors in regulating plant-insect interactions.Here,we report that a microRNA(miR29-b)found in the saliva of the phloem-feeding whitefly(Bemisia tabaci)can transfer into the host plant phloem during feeding and fine-tune the defense response of tobacco(Nicotiana tabacum)plants.We show that the salivary gland-enriched BtmiR29-b is produced by BtDicer 1 and released into tobacco cells via salivary exosomes.Once inside the plant cells,BtmiR29-b hijacks tobacco Argonaute 1 to silence the defense gene Bcl-2-associated athanogene 4(NtBAG4).In tobacco,NtBAG4 acts as the positive regulator of phytohormones salicylic acid(SA)and jasmonic acid(JA),enhancing plant defense against whitefly attacks.Interestingly,we also found that miR29-b acts as a salivary effector in another Hemipteran insect,the aphid Myzus persicae,which inhibits tobacco resistance by degrading NtBAG4.Moreover,miR29-b is highly conserved in Hemiptera and across other insect orders such as Coleoptera,Hymenoptera,Orthoptera,and Blattaria.Computational analysis suggests that miR29-b may also target the evolutionarily conserved BAG4 gene in other plant species.We further provide evidence showing BtmiR29-b-mediated BAG4 cleavage and defense suppression in tomato(Solanum lycopersicum).Taken together,our work reveals that a conserved miR29-b effector from insects fine-tunes plant SA-and JA-mediated defense by cross-kingdom silencing of the host plant BAG4 gene,providing new insight into the defense and counter-defense mechanisms between herbivores and their host plants.
基金support of the following funding sources:the Young Talent Program of the Affiliated Hospital of Xuzhou Medical University,the Advanced Program of the Affiliated Hospital of Xuzhou Medical University(PYJH2024210)Xuzhou City Clinical Technical Backbone Training Program Project(2025211001)+6 种基金the Key Medical Talent Program of Xuzhou City(XWRCHT20220065)the Noncommunicable Chronic Diseases-National Science and Technology Major Project(No.2023ZD0502300[2023ZD0502304])the Medical Research Project of Jiangsu Provincial Health Commission(H2023050)the National Natural Science Foundation of China(No.32301278)the Sichuan Provincial Department of Science and Technology(2023YFH0095)the Technological Innovation Research Project of Chengdu Science and Technology Bureau(2024-YF05-00723-SN)the National Guidance Fund on Developing Local Science and Technology for Sichuan Province(No.2023ZYD0167).
文摘Chemotherapy serves as the primary therapeutic approach for triple-negative breast cancer(TNBC),yet its efficacy remains unsatisfactory.This study was a single-arm,open-label,single-center clinical trial(NCT05447702)involving patients with newly diagnosed stage Ⅱ-Ⅲ TNBC at West China Hospital.The treatment regimen consisted of camrelizumab(200 mg intravenously every 2 weeks,12 cycles),apatinib(250 mg orally daily),and alternating chemotherapy[nab-paclitaxel(d1,8,15 every 4 weeks)for 4 cycles and epirubicin plus cyclophosphamide(every 2 weeks)for 4 cycles].From June 2023 to April 2024,35 patients were enrolled,of whom 1 patient withdrew due to adverse reaction intolerance.At treatment completion,the total pathological complete response(tpCR,ypT0/is,ypN0)rate was 67.6%(23/34),and the breast pCR(ypT0/is)rate was 70.6%(24/34).The overall response rate following neoadjuvant treatment reached 94.1%(32/34).
