Prirnula interjacens Chen (Primulaceae) is a rare and narrow endemic species of centralsouth of Yunnan Province in China. This species consists of two varieties: P.interjacens var. interjacens known with only one popu...Prirnula interjacens Chen (Primulaceae) is a rare and narrow endemic species of centralsouth of Yunnan Province in China. This species consists of two varieties: P.interjacens var. interjacens known with only one population, and P.interjacens var. epilosa with two populations. Intersimple sequence repeat (ISSR) marker was used to detect the genetic diversity of the three extant populations. We expected a low genetic diversity level, but our results revealed a high level of intraspecific genetic diversity (at population level: P=59.75%, HE=0.2368, and Hpop=0.3459; at species level: P=75.47%, HT= 0.320 5, and Hsp = 0.4618), probably resulting from floral heteromorphism and preferring outcrossing. A moderate level of genetic differentiation among populations was detected based on Nei's genetic diversity analysis (26.13%) and Shannon's diversity index (25.09%). Although P./ntedacens var. intedacens and P. interjacens var. epilosa were morphologically distinct, UPGMA cluster analysis showed that the two varieties had no distinct genetic differentiation and may be treated as a single taxon. Conservation measures are suggested, including in situ and ex situ strategies, based on the observed population genetic information.展开更多
Three new C21 steroidal glycosides named komaroside A, komaroside B, komaroside C were isolated from the ethanolic extract of the roots of Cynanchum komarovii Al.Iljinski (Asclepiadaceae), their structures were determ...Three new C21 steroidal glycosides named komaroside A, komaroside B, komaroside C were isolated from the ethanolic extract of the roots of Cynanchum komarovii Al.Iljinski (Asclepiadaceae), their structures were determined by physiochemical and spectroscopic analysis.展开更多
A new C-18 phytosphingosine derivative (1) was isolated from the fruiting bodies of Tuber indicum. Its structure was established as (2S, 3S, 4R, 2R)-2-N-(2-hydroxytricosano- yl)-octadecan-1, 3, 4-triol by spectral a...A new C-18 phytosphingosine derivative (1) was isolated from the fruiting bodies of Tuber indicum. Its structure was established as (2S, 3S, 4R, 2R)-2-N-(2-hydroxytricosano- yl)-octadecan-1, 3, 4-triol by spectral and chemical methods.展开更多
Two new tetranortriterpenoids 3-oxo-24, 25, 26, 27-tetranortirucall-7-ene-23(21)- lactone, 3á-hydroxy-24, 25, 26, 27-tetranortirucall-7-ene-23(21)-lactone were isolated from Amoora dasyclada. Their structures wer...Two new tetranortriterpenoids 3-oxo-24, 25, 26, 27-tetranortirucall-7-ene-23(21)- lactone, 3á-hydroxy-24, 25, 26, 27-tetranortirucall-7-ene-23(21)-lactone were isolated from Amoora dasyclada. Their structures were elucidated by spectroscopic evidences.展开更多
Bioactivity-guided fractionation led to the isolation of a new steroidal saponin, orbiculatoside B, together with a pair of furostanol saponins, protobioside and methyl protobioside, from the fresh rhizomes of Dioscor...Bioactivity-guided fractionation led to the isolation of a new steroidal saponin, orbiculatoside B, together with a pair of furostanol saponins, protobioside and methyl protobioside, from the fresh rhizomes of Dioscorea deltoidea Wall var. orbiculata. The new compound was identified to be isonarthogenin 3 O - a - L - rhamnopyranosyl (12) - [ a - L - rhamnopyranosyl - ( 1 4 ) ] - b - D - glucopyranoside by various NMR techniques in combination with chemical methods. The three saponins showed strong activity against Pyricularia oryzae, and were cytotoxic to cancer cell line K562, HCT-15, A549, and A2780a in vitro.展开更多
Two new labdane diterpenes isolated from the rhizomes of Hedychium forrestii were determined by spectroscopic evidence to be labda-8(17), 11, 13-trien-7β-hydroxyl-15(16)-olide (1, hedyforrestin B) and labda-8(17), 11...Two new labdane diterpenes isolated from the rhizomes of Hedychium forrestii were determined by spectroscopic evidence to be labda-8(17), 11, 13-trien-7β-hydroxyl-15(16)-olide (1, hedyforrestin B) and labda-8(17), 11, 13-trien-7β,16-dihydroxyl-16(15)-olide (2, hedyforrestin C).展开更多
Two new monoterpene diglycosides, wincaloside A (1) and wincaloside B (2), along with loganin (3) were isolated from the stem barks of Winchia calophylla A. DC. The structures of 1 and 2 were established by spectrosco...Two new monoterpene diglycosides, wincaloside A (1) and wincaloside B (2), along with loganin (3) were isolated from the stem barks of Winchia calophylla A. DC. The structures of 1 and 2 were established by spectroscopic and chemical methods.展开更多
Three new diarylpropanes were isolated from the dried rhizomes of Dioscorea composita Hemsl., and their structures were determined as 1, 3-bis-(2-hydroxy-4-methoxyphenyl) propane (1), 1, 3-bis-(2, 4-dihydroxyphenyl)...Three new diarylpropanes were isolated from the dried rhizomes of Dioscorea composita Hemsl., and their structures were determined as 1, 3-bis-(2-hydroxy-4-methoxyphenyl) propane (1), 1, 3-bis-(2, 4-dihydroxyphenyl)propane (2), 1-(2′-hydroxy-4′-O-β-D- glucopyranosy- phenyl)-3-(2′′, 4′′-dihydroxyphenyl)propane (3), by spectroscopic analysis, respectively.展开更多
Two new pyrrolizidines named lankongensisine A (1), B (2) were isolated from the roots of Ligularia lankongensis collected in Lijiang, Yunnan, and their structures were established by spectroscopic analysis.
Two new ent-kauranoids, named maoyecrystals A (1) and B (2), were isolated from the EtOAc extract of the dried leaves of Isodon japonica (Burman f.) Hara collected in Tongbai mountains, Henan Province. Their structur...Two new ent-kauranoids, named maoyecrystals A (1) and B (2), were isolated from the EtOAc extract of the dried leaves of Isodon japonica (Burman f.) Hara collected in Tongbai mountains, Henan Province. Their structures were determined on the basis of spectral data, especially by 2D NMR.展开更多
The relationship between LOX(lipoxygenase)activity, SA(salicylic acid)and JA(jasmonic acid)accumulation was studied in the tobacco leaf under water stress. The result showed that LOX activity and JA content increased ...The relationship between LOX(lipoxygenase)activity, SA(salicylic acid)and JA(jasmonic acid)accumulation was studied in the tobacco leaf under water stress. The result showed that LOX activity and JA content increased simutaneously with losing of water . NDGA(nordihydroguaiaretic),an inhibitor of LOX, inhibited simultaneously both the activity of LOX and the enhancement of JA level under the stress. Likewise, adding LOX to tobacco cell-free system led to the increase of JA content. It suggested that LOX could be a key enzyme in JA biosynthesis under water stress. SA inhibited the enhancement of JA level under the stress.展开更多
From the dried aerial part of Parepigynum funingesis Tsiang et P. T. Li (Apocynaceae), a new cardiac glucoside, named parefuningoside (1) had been isolated. Its structure was determined by means of hydrolysis and spec...From the dried aerial part of Parepigynum funingesis Tsiang et P. T. Li (Apocynaceae), a new cardiac glucoside, named parefuningoside (1) had been isolated. Its structure was determined by means of hydrolysis and spectral analysis.展开更多
A new sesquiterpenoid was obstained from the leaves of Magnolia delavayi. Its structure was determined as 8β-acetoxy-10α-ethyloxy-guaia-4α, 11-diol on the basis of spectral evidence.
Two new ent-kaurane diterpenoids, sculponeatins L (1) and M (2), were isolated from the EtOAc extract of Isodon sculponeata. Their structures were elucidated by spectroscopic evidences. The cytotoxicities of 1 and 2...Two new ent-kaurane diterpenoids, sculponeatins L (1) and M (2), were isolated from the EtOAc extract of Isodon sculponeata. Their structures were elucidated by spectroscopic evidences. The cytotoxicities of 1 and 2 against human tumor cells K562 and T24 were tested.展开更多
A novel saponin was isolated from the transformed products of ginsenoside Rh1 by Bacillus subtilis. It's structure was determined to be 3-O-β-D-glucopyranosyl-6-O-β-D-gluco- pyranosyl-20 (S)-protopanaxatriol on ...A novel saponin was isolated from the transformed products of ginsenoside Rh1 by Bacillus subtilis. It's structure was determined to be 3-O-β-D-glucopyranosyl-6-O-β-D-gluco- pyranosyl-20 (S)-protopanaxatriol on the basis of the spectral data.展开更多
Two new eremophilanolides, tsoongianolide E and F, were isolated from Senecio tsoongianus. Their structures were elucidated on the basis of NMR and MS spectra. Both of these two compounds showed in vitro cytotoxicity ...Two new eremophilanolides, tsoongianolide E and F, were isolated from Senecio tsoongianus. Their structures were elucidated on the basis of NMR and MS spectra. Both of these two compounds showed in vitro cytotoxicity to cultured KB and A-549 cancer cell lines.展开更多
Two new compounds, oleanolic acid 28-O--D-glucopyranosyl-3-O--L-arabinopyrano- syl-(13)-(6-butyl ester)--D-glucuropyranoside 1, oleanolic acid 28-O--D-glucopyranosyl- (16)--D-glucopyranoside 2 have been isolated from ...Two new compounds, oleanolic acid 28-O--D-glucopyranosyl-3-O--L-arabinopyrano- syl-(13)-(6-butyl ester)--D-glucuropyranoside 1, oleanolic acid 28-O--D-glucopyranosyl- (16)--D-glucopyranoside 2 have been isolated from the roots of Hemsleya penxianensis var. gulinensis. Their structures were determined on the bases of the spectral and chemical evidences.展开更多
Hedychenone (1), a diterpene containing a, B-unsaturated keto group, was reduced by aluminum-mercury alloy, and a dimerized product (2) was obtained as the major product. The coupling occurred at B position of the ket...Hedychenone (1), a diterpene containing a, B-unsaturated keto group, was reduced by aluminum-mercury alloy, and a dimerized product (2) was obtained as the major product. The coupling occurred at B position of the keto group.展开更多
A dimer of styrylpyrone derivative, leiocarpin E (1), was isolated from the bark of Goniothalamus leiocarpus. Its structures was elucidated by means of spectral and chemical methods. The cytotoxicity of leiocarpin E ...A dimer of styrylpyrone derivative, leiocarpin E (1), was isolated from the bark of Goniothalamus leiocarpus. Its structures was elucidated by means of spectral and chemical methods. The cytotoxicity of leiocarpin E against HL-60 cells was tested.展开更多
文摘Prirnula interjacens Chen (Primulaceae) is a rare and narrow endemic species of centralsouth of Yunnan Province in China. This species consists of two varieties: P.interjacens var. interjacens known with only one population, and P.interjacens var. epilosa with two populations. Intersimple sequence repeat (ISSR) marker was used to detect the genetic diversity of the three extant populations. We expected a low genetic diversity level, but our results revealed a high level of intraspecific genetic diversity (at population level: P=59.75%, HE=0.2368, and Hpop=0.3459; at species level: P=75.47%, HT= 0.320 5, and Hsp = 0.4618), probably resulting from floral heteromorphism and preferring outcrossing. A moderate level of genetic differentiation among populations was detected based on Nei's genetic diversity analysis (26.13%) and Shannon's diversity index (25.09%). Although P./ntedacens var. intedacens and P. interjacens var. epilosa were morphologically distinct, UPGMA cluster analysis showed that the two varieties had no distinct genetic differentiation and may be treated as a single taxon. Conservation measures are suggested, including in situ and ex situ strategies, based on the observed population genetic information.
文摘Three new C21 steroidal glycosides named komaroside A, komaroside B, komaroside C were isolated from the ethanolic extract of the roots of Cynanchum komarovii Al.Iljinski (Asclepiadaceae), their structures were determined by physiochemical and spectroscopic analysis.
基金supported by the Natural Science Foundation of Yunnan Province(2000B0066M)
文摘A new C-18 phytosphingosine derivative (1) was isolated from the fruiting bodies of Tuber indicum. Its structure was established as (2S, 3S, 4R, 2R)-2-N-(2-hydroxytricosano- yl)-octadecan-1, 3, 4-triol by spectral and chemical methods.
文摘Two new tetranortriterpenoids 3-oxo-24, 25, 26, 27-tetranortirucall-7-ene-23(21)- lactone, 3á-hydroxy-24, 25, 26, 27-tetranortirucall-7-ene-23(21)-lactone were isolated from Amoora dasyclada. Their structures were elucidated by spectroscopic evidences.
文摘Bioactivity-guided fractionation led to the isolation of a new steroidal saponin, orbiculatoside B, together with a pair of furostanol saponins, protobioside and methyl protobioside, from the fresh rhizomes of Dioscorea deltoidea Wall var. orbiculata. The new compound was identified to be isonarthogenin 3 O - a - L - rhamnopyranosyl (12) - [ a - L - rhamnopyranosyl - ( 1 4 ) ] - b - D - glucopyranoside by various NMR techniques in combination with chemical methods. The three saponins showed strong activity against Pyricularia oryzae, and were cytotoxic to cancer cell line K562, HCT-15, A549, and A2780a in vitro.
基金Program granted by the Laboratory of Phytochemstry,Kunming Institute of Botany,the Chinese Academy of Sciences
文摘Two new labdane diterpenes isolated from the rhizomes of Hedychium forrestii were determined by spectroscopic evidence to be labda-8(17), 11, 13-trien-7β-hydroxyl-15(16)-olide (1, hedyforrestin B) and labda-8(17), 11, 13-trien-7β,16-dihydroxyl-16(15)-olide (2, hedyforrestin C).
基金supported by the National Natural Science Foundation for Outstanding Young Scientists to Prof.Xiao Jiang Hao(No.39525025).
文摘Two new monoterpene diglycosides, wincaloside A (1) and wincaloside B (2), along with loganin (3) were isolated from the stem barks of Winchia calophylla A. DC. The structures of 1 and 2 were established by spectroscopic and chemical methods.
基金supported by the Natural Science foundation of Yunnan(YK98002)
文摘Three new diarylpropanes were isolated from the dried rhizomes of Dioscorea composita Hemsl., and their structures were determined as 1, 3-bis-(2-hydroxy-4-methoxyphenyl) propane (1), 1, 3-bis-(2, 4-dihydroxyphenyl)propane (2), 1-(2′-hydroxy-4′-O-β-D- glucopyranosy- phenyl)-3-(2′′, 4′′-dihydroxyphenyl)propane (3), by spectroscopic analysis, respectively.
文摘Two new pyrrolizidines named lankongensisine A (1), B (2) were isolated from the roots of Ligularia lankongensis collected in Lijiang, Yunnan, and their structures were established by spectroscopic analysis.
文摘Two new ent-kauranoids, named maoyecrystals A (1) and B (2), were isolated from the EtOAc extract of the dried leaves of Isodon japonica (Burman f.) Hara collected in Tongbai mountains, Henan Province. Their structures were determined on the basis of spectral data, especially by 2D NMR.
文摘The relationship between LOX(lipoxygenase)activity, SA(salicylic acid)and JA(jasmonic acid)accumulation was studied in the tobacco leaf under water stress. The result showed that LOX activity and JA content increased simutaneously with losing of water . NDGA(nordihydroguaiaretic),an inhibitor of LOX, inhibited simultaneously both the activity of LOX and the enhancement of JA level under the stress. Likewise, adding LOX to tobacco cell-free system led to the increase of JA content. It suggested that LOX could be a key enzyme in JA biosynthesis under water stress. SA inhibited the enhancement of JA level under the stress.
文摘From the dried aerial part of Parepigynum funingesis Tsiang et P. T. Li (Apocynaceae), a new cardiac glucoside, named parefuningoside (1) had been isolated. Its structure was determined by means of hydrolysis and spectral analysis.
文摘A new sesquiterpenoid was obstained from the leaves of Magnolia delavayi. Its structure was determined as 8β-acetoxy-10α-ethyloxy-guaia-4α, 11-diol on the basis of spectral evidence.
文摘Two new ent-kaurane diterpenoids, sculponeatins L (1) and M (2), were isolated from the EtOAc extract of Isodon sculponeata. Their structures were elucidated by spectroscopic evidences. The cytotoxicities of 1 and 2 against human tumor cells K562 and T24 were tested.
文摘A novel saponin was isolated from the transformed products of ginsenoside Rh1 by Bacillus subtilis. It's structure was determined to be 3-O-β-D-glucopyranosyl-6-O-β-D-gluco- pyranosyl-20 (S)-protopanaxatriol on the basis of the spectral data.
文摘Two new eremophilanolides, tsoongianolide E and F, were isolated from Senecio tsoongianus. Their structures were elucidated on the basis of NMR and MS spectra. Both of these two compounds showed in vitro cytotoxicity to cultured KB and A-549 cancer cell lines.
基金supported by Natural Science Foundation of Yunnan and National Natural Science Foundation of China (Grant No.98C089M and No.39970086). The authors were grateful to the Departmental of Instrument Analysis Kunming Institute of Botany Chinese Academy o
文摘Two new compounds, oleanolic acid 28-O--D-glucopyranosyl-3-O--L-arabinopyrano- syl-(13)-(6-butyl ester)--D-glucuropyranoside 1, oleanolic acid 28-O--D-glucopyranosyl- (16)--D-glucopyranoside 2 have been isolated from the roots of Hemsleya penxianensis var. gulinensis. Their structures were determined on the bases of the spectral and chemical evidences.
文摘Hedychenone (1), a diterpene containing a, B-unsaturated keto group, was reduced by aluminum-mercury alloy, and a dimerized product (2) was obtained as the major product. The coupling occurred at B position of the keto group.
文摘A dimer of styrylpyrone derivative, leiocarpin E (1), was isolated from the bark of Goniothalamus leiocarpus. Its structures was elucidated by means of spectral and chemical methods. The cytotoxicity of leiocarpin E against HL-60 cells was tested.
