The extent and patterns of cytosine methylation in blood DNA were assessed, using thetechnique of methylation-sensitive amplified polymorphism(MSAP),in Meishan, Large White pigsand hybrids of their reciprocal crosses....The extent and patterns of cytosine methylation in blood DNA were assessed, using thetechnique of methylation-sensitive amplified polymorphism(MSAP),in Meishan, Large White pigsand hybrids of their reciprocal crosses. In all, 1 508 fragments, each representing a reco-gnition site cleaved by either or both of the isoschizomers, MspI and HpaII, were amplifiedusing 20 pairs of selective primers. 10.3% of CCGG sites were methylated in Meishan pigs, 10.5%in Large White pigs, and 10.2% in the hybrids. Cytosine methylation was not significantlydifferent among parental lines and hybrids of reciprocal crosses. Four classes of patternswere identified in a comparative assay of cytosine methylation in the parents and hybrids: (1)the same level of methylation in both parental lines and the hybrids; (2) the same level ofmethylation in either parent or hybrid; (3) an increased level of methylation in the hybridscompared to the parents, and (4) a decreased level of methylation in the hybrids. 11 cross-specific methylation sites were detected in F1 hybrids of Large White ×Meishan, and 10 cross-specific methylation sites in the hybrid of Meishan ×Large White. In conclusion, (1) the wholemethylation status between parental lines and hybrids was not different, but specific siteswere differentially methylated; (2) specific sites were differentially methylated betweenreciprocal crosses; (3) demethylation and hypermethylation of many sites accounted for mostly(more than 50%) methylated sites in the hybrids compared to parental lines.展开更多
In order to detect the molecular mechanism of heterosis in pigs, the mRNA differential display technique was performed to investigate the differences of gene expression in the Longissimus dorsi tissue from Meishan, ...In order to detect the molecular mechanism of heterosis in pigs, the mRNA differential display technique was performed to investigate the differences of gene expression in the Longissimus dorsi tissue from Meishan, Meishan × Large White hybrid and Large White pigs with nine 3'-end anchored primers in combination with ten 5'-end arbitrary primers and nearly 3000 reproducible bands were examined. One novel expressed sequence tag (EST4, GenBank accession number: AY553914) that was differentially expressed in Meishan, Meishan× Large White hybrid and Large White pigs was isolated from the Longissimus dorsi muscle tissue and identified through semi-quantitative RT-PCR. BLAST analysis revealed that the 350 bp long EST (EST4) was not homologous to any of the known porcine genes. Tissue expression profile analyses showed that the EST4 was expressed in most of tissues.LIU Yong-gang, Ph D candidate展开更多
A Hinf Ⅰ locus of the porcine subunit C of succinate dehydrogenase complex (SDHC) gene and a Msp Ⅰ locus of theporcine rod cGMP-phosphodiesterase γ-subunit (PDE6G) gene had been reported before, but the association...A Hinf Ⅰ locus of the porcine subunit C of succinate dehydrogenase complex (SDHC) gene and a Msp Ⅰ locus of theporcine rod cGMP-phosphodiesterase γ-subunit (PDE6G) gene had been reported before, but the association analysisbetween the different genotypes and the traits had not been done. 300 Large White × Meishan F2 pigs were used asexperimental materials to performe the PCR-RFLP analysis and association analysis for the two loci, results revealed thatthe polymorphism of the porcine subunit C of succinate dehydrogenase complex (SDHC) gene was significantly associatedwith the traits which included the carcass length, the estimated lean meat percentage, the estimated backfat thickness atlast rib, the estimated backfat thickness at last 3-4th rib, the fat meat weight, the fat meat percentage, the lean meat weight,the lean meat percentage, the ratio of lean meat to fat meat, the leaf fat weight, the backfat thickness at shoulder, thebackfat thickness at thorax-Waist, the backfat thickness at 6-7th thorax and the average daily gain. Seven other traits, themeat color value (Biceps femoris, BF), the meat marbling (Biceps femoris, BF), the water moisture (Longissimus dorsi, LD),the bone weight, the bone percentage, the loin eye width and the loin eye area, were found to be significantly correlatedwith the polymorphism of the porcine rod cGMP-phosphodiesterase γ-subunit (PDE6G) gene. Based on these results, itis necessary to apply the two genes as candidate genes to marker assistant selection (MAS) in pig breeding.展开更多
In order to detect the molecular mechanism of heterosis in pigs, the mRNA differential display technique was performedto investigate the differences of gene expression in the backfat tissue from Meishan, Large White a...In order to detect the molecular mechanism of heterosis in pigs, the mRNA differential display technique was performedto investigate the differences of gene expression in the backfat tissue from Meishan, Large White and MeishanLargeWhite cross pigs. Nine 3'-end anchored primers in combination with ten 5'-end arbitrary primers were used to perform thedifferential display PCR and nearly 3 000 reproducible bands were examined. Fifteen expressed sequence tags that weredifferentially expressed were isolated and then identified through semi-quantitative RT-PCR. BLAST analysis revealedthat the fifteen expressed sequence tags (ESTs) were not homologous to any of the known porcine genes or ESTs. Thesenovel ESTs were then submitted to GenBank.展开更多
文摘The extent and patterns of cytosine methylation in blood DNA were assessed, using thetechnique of methylation-sensitive amplified polymorphism(MSAP),in Meishan, Large White pigsand hybrids of their reciprocal crosses. In all, 1 508 fragments, each representing a reco-gnition site cleaved by either or both of the isoschizomers, MspI and HpaII, were amplifiedusing 20 pairs of selective primers. 10.3% of CCGG sites were methylated in Meishan pigs, 10.5%in Large White pigs, and 10.2% in the hybrids. Cytosine methylation was not significantlydifferent among parental lines and hybrids of reciprocal crosses. Four classes of patternswere identified in a comparative assay of cytosine methylation in the parents and hybrids: (1)the same level of methylation in both parental lines and the hybrids; (2) the same level ofmethylation in either parent or hybrid; (3) an increased level of methylation in the hybridscompared to the parents, and (4) a decreased level of methylation in the hybrids. 11 cross-specific methylation sites were detected in F1 hybrids of Large White ×Meishan, and 10 cross-specific methylation sites in the hybrid of Meishan ×Large White. In conclusion, (1) the wholemethylation status between parental lines and hybrids was not different, but specific siteswere differentially methylated; (2) specific sites were differentially methylated betweenreciprocal crosses; (3) demethylation and hypermethylation of many sites accounted for mostly(more than 50%) methylated sites in the hybrids compared to parental lines.
文摘In order to detect the molecular mechanism of heterosis in pigs, the mRNA differential display technique was performed to investigate the differences of gene expression in the Longissimus dorsi tissue from Meishan, Meishan × Large White hybrid and Large White pigs with nine 3'-end anchored primers in combination with ten 5'-end arbitrary primers and nearly 3000 reproducible bands were examined. One novel expressed sequence tag (EST4, GenBank accession number: AY553914) that was differentially expressed in Meishan, Meishan× Large White hybrid and Large White pigs was isolated from the Longissimus dorsi muscle tissue and identified through semi-quantitative RT-PCR. BLAST analysis revealed that the 350 bp long EST (EST4) was not homologous to any of the known porcine genes. Tissue expression profile analyses showed that the EST4 was expressed in most of tissues.LIU Yong-gang, Ph D candidate
文摘A Hinf Ⅰ locus of the porcine subunit C of succinate dehydrogenase complex (SDHC) gene and a Msp Ⅰ locus of theporcine rod cGMP-phosphodiesterase γ-subunit (PDE6G) gene had been reported before, but the association analysisbetween the different genotypes and the traits had not been done. 300 Large White × Meishan F2 pigs were used asexperimental materials to performe the PCR-RFLP analysis and association analysis for the two loci, results revealed thatthe polymorphism of the porcine subunit C of succinate dehydrogenase complex (SDHC) gene was significantly associatedwith the traits which included the carcass length, the estimated lean meat percentage, the estimated backfat thickness atlast rib, the estimated backfat thickness at last 3-4th rib, the fat meat weight, the fat meat percentage, the lean meat weight,the lean meat percentage, the ratio of lean meat to fat meat, the leaf fat weight, the backfat thickness at shoulder, thebackfat thickness at thorax-Waist, the backfat thickness at 6-7th thorax and the average daily gain. Seven other traits, themeat color value (Biceps femoris, BF), the meat marbling (Biceps femoris, BF), the water moisture (Longissimus dorsi, LD),the bone weight, the bone percentage, the loin eye width and the loin eye area, were found to be significantly correlatedwith the polymorphism of the porcine rod cGMP-phosphodiesterase γ-subunit (PDE6G) gene. Based on these results, itis necessary to apply the two genes as candidate genes to marker assistant selection (MAS) in pig breeding.
文摘In order to detect the molecular mechanism of heterosis in pigs, the mRNA differential display technique was performedto investigate the differences of gene expression in the backfat tissue from Meishan, Large White and MeishanLargeWhite cross pigs. Nine 3'-end anchored primers in combination with ten 5'-end arbitrary primers were used to perform thedifferential display PCR and nearly 3 000 reproducible bands were examined. Fifteen expressed sequence tags that weredifferentially expressed were isolated and then identified through semi-quantitative RT-PCR. BLAST analysis revealedthat the fifteen expressed sequence tags (ESTs) were not homologous to any of the known porcine genes or ESTs. Thesenovel ESTs were then submitted to GenBank.
