The effects of osmotic stress on the ATPase activity, the contents of —SH group and conjugated polyamines in mitochondrial membrane from wheat seedling [Triticum aestivum L. cv. Yumai No.18(drought-tolerant) and cv. ...The effects of osmotic stress on the ATPase activity, the contents of —SH group and conjugated polyamines in mitochondrial membrane from wheat seedling [Triticum aestivum L. cv. Yumai No.18(drought-tolerant) and cv. Yumai No.9(drought-sensitive)] roots were investigated. The results showed that ATPase activity and —SH group content decreased with polyethylene glycol(PEG) 6000(-0.55 MPa) treatment for 7 d, in concert with the decrease of the ratio of noncovalently conjugated spermidine(NCC-Spd)/noncovalently conjugated putrescine(NCC-Put) and increase of the covalently conjugated putrescine(CC-Put). Osmotic stress injury to Yangmai No.9 seedlings was alleviated greatly with 1 mmol/L exogenous spermidine(Spd), in concert with marked increases of the ratio of NCC-Spd/NCC-Put, —SH group contents and ATPase activity in mitochondrial membrane. Under osmotic stress, the concomitant treatment of Yumai No.18 seedlings with methylglyoxyl bis(guanylhydrazone) (MGBG), an inhibitor of S-adenosyl methionine decarboxylase(SAMDC), and phenanthrolin (o-Phen), an inhibitor of transglutaminase(TGase), caused a significant decrease of the ratio of NCC-Spd / NCC-Put, CC-Put contents, respectively, in concert with the marked decreases of ATPase activity, —SH group content and its tolerance to osmotic stress. All the results above suggested that osmotic stress tolerance of wheat seedlings was associated with the ATPase activity, the contents of —SH group, NCC-Spd and CC-Put in mitochondrial membrane.展开更多
The investigation was made on the relationship of seasonal time-course canopy spectral reflectance and ratio index to total leaf nitrogen accumulation (leaf nitrogen content per unit ground area) in rice under differe...The investigation was made on the relationship of seasonal time-course canopy spectral reflectance and ratio index to total leaf nitrogen accumulation (leaf nitrogen content per unit ground area) in rice under different nitrogen treatments. The results showed there was a close correlation between the canopy spectral reflectance and total leaf nitrogen accumulation. Ratio of near infrared to green band (R810/R560) was linearly related with total leaf nitrogen accumulation. independent of nitrogen levels and development stages. Different datasets were used to test the linear regression equation, with average estimation accuracy of 91. 22%, RMSE of 1.09 and average relative error of 0. 026. Thus, the ratio index R810/R560 of canopy spectral reflectance should be useful for non-destructive monitoring and diagnosis of nitrogen status in rice plants.展开更多
The influence of major cultural practices including different nitrogen application rates, population densities, transplanting leaf ages of seedling, and water regimes on rice canopy spectral reflectance was investigat...The influence of major cultural practices including different nitrogen application rates, population densities, transplanting leaf ages of seedling, and water regimes on rice canopy spectral reflectance was investigated. Results showed that increased nitrogen rates, water regimes and population densities and decreased seedling ages could enhance reflectance at NIR （near infrared） bands and reduce reflectance at visible bands. Using reflectance of green, red and NIR band and ratio index of 810-560 nm could distinguish the different type of rice by fuzzy cluster analysis,展开更多
文摘The effects of osmotic stress on the ATPase activity, the contents of —SH group and conjugated polyamines in mitochondrial membrane from wheat seedling [Triticum aestivum L. cv. Yumai No.18(drought-tolerant) and cv. Yumai No.9(drought-sensitive)] roots were investigated. The results showed that ATPase activity and —SH group content decreased with polyethylene glycol(PEG) 6000(-0.55 MPa) treatment for 7 d, in concert with the decrease of the ratio of noncovalently conjugated spermidine(NCC-Spd)/noncovalently conjugated putrescine(NCC-Put) and increase of the covalently conjugated putrescine(CC-Put). Osmotic stress injury to Yangmai No.9 seedlings was alleviated greatly with 1 mmol/L exogenous spermidine(Spd), in concert with marked increases of the ratio of NCC-Spd/NCC-Put, —SH group contents and ATPase activity in mitochondrial membrane. Under osmotic stress, the concomitant treatment of Yumai No.18 seedlings with methylglyoxyl bis(guanylhydrazone) (MGBG), an inhibitor of S-adenosyl methionine decarboxylase(SAMDC), and phenanthrolin (o-Phen), an inhibitor of transglutaminase(TGase), caused a significant decrease of the ratio of NCC-Spd / NCC-Put, CC-Put contents, respectively, in concert with the marked decreases of ATPase activity, —SH group content and its tolerance to osmotic stress. All the results above suggested that osmotic stress tolerance of wheat seedlings was associated with the ATPase activity, the contents of —SH group, NCC-Spd and CC-Put in mitochondrial membrane.
基金supported by the National Natural Science Fundation of China(30030090)National Major Basic Research Proiect(G2000077900).
文摘The investigation was made on the relationship of seasonal time-course canopy spectral reflectance and ratio index to total leaf nitrogen accumulation (leaf nitrogen content per unit ground area) in rice under different nitrogen treatments. The results showed there was a close correlation between the canopy spectral reflectance and total leaf nitrogen accumulation. Ratio of near infrared to green band (R810/R560) was linearly related with total leaf nitrogen accumulation. independent of nitrogen levels and development stages. Different datasets were used to test the linear regression equation, with average estimation accuracy of 91. 22%, RMSE of 1.09 and average relative error of 0. 026. Thus, the ratio index R810/R560 of canopy spectral reflectance should be useful for non-destructive monitoring and diagnosis of nitrogen status in rice plants.
文摘The influence of major cultural practices including different nitrogen application rates, population densities, transplanting leaf ages of seedling, and water regimes on rice canopy spectral reflectance was investigated. Results showed that increased nitrogen rates, water regimes and population densities and decreased seedling ages could enhance reflectance at NIR （near infrared） bands and reduce reflectance at visible bands. Using reflectance of green, red and NIR band and ratio index of 810-560 nm could distinguish the different type of rice by fuzzy cluster analysis,
