With the development of computational methods and RNA sequencing technology for assembling the transcriptome, it is becoming clear that the mammal genome is pervasively tran- scribed, and large numbers of long noncodi...With the development of computational methods and RNA sequencing technology for assembling the transcriptome, it is becoming clear that the mammal genome is pervasively tran- scribed, and large numbers of long noncoding RNAs （lncRNAs） composing the major part of the transcriptome have been identified （Ravasi et al., 2006; Birney et al., 2007;展开更多
In the past two decades,various super-resolution(SR)microscopy techniques have been developed to break the di®raction limit using subdi®raction excitation to spatially modulate the°uorescence emission.P...In the past two decades,various super-resolution(SR)microscopy techniques have been developed to break the di®raction limit using subdi®raction excitation to spatially modulate the°uorescence emission.Photomodulatable°uorescent proteins(FPs)can be activated by light of speci¯c wavelengths to produce either stochastic or patterned subdi®raction excitation,resulting in improved optical resolution.In this review,we focus on the recently developed photomodulatable FPs or commonly used SR microscopies and discuss the concepts and strategies for optimizing and selecting the biochemical and photophysical properties of PMFPs to improve the spatiotemporal resolution of SR techniques,especially time-lapse live-cell SR techniques.展开更多
Apparently,the genomes of many organisms are pervasively transcribed,and long noncoding RNAs(lncRNAs)make up the majority of cellular transcripts.LncRNAs have been reported to play important roles in many biological p...Apparently,the genomes of many organisms are pervasively transcribed,and long noncoding RNAs(lncRNAs)make up the majority of cellular transcripts.LncRNAs have been reported to play important roles in many biological processes;however,their effects on locomotion are poorly understood.Here,we presented a novel lncRNA,Locomotion Regulatory Gene(LRG),which participates in locomotion by sequestering Synaptotagmin 1(SYT1).LRG deficiency resulted in higher locomotion speed which could be rescued by pan-neuronal overexpression but not by limited ellipsoid body,motoneuron or muscle-expression of LRG.At the molecular level,the synaptic vesicles(SVs)release and movement-related SYT1 protein was recognized as LRG-interacting protein candidate.Furthermore,LRG had no effects on SYT1 expression.Genetically,the behavioral defects in LRG mutant could be rescued by pan-neuronal knock-down of Syt1.Taken together,all the results suggested LRG exerts regulatory effects on locomotion via sequestering SYT1 thereby blocking its function without affecting its expression.Our work displays a new function of lncRNA and provides insights for revealing the pathogenesis of neurological diseases with motor disorders.展开更多
基金supported by the grants from the National Natural Science Foundation of China (No. 31300889)
文摘With the development of computational methods and RNA sequencing technology for assembling the transcriptome, it is becoming clear that the mammal genome is pervasively tran- scribed, and large numbers of long noncoding RNAs （lncRNAs） composing the major part of the transcriptome have been identified （Ravasi et al., 2006; Birney et al., 2007;
基金This project was supported by the National Basic Research Program (2013CB910103)the National Natural Science Foundation of China (Grant Nos.31421002,31370851 and 31300612)+1 种基金the Project of the Chinese Academy of Sciences (XDB08030202)the Beijing Natural Science Foundation (7131011)。
文摘In the past two decades,various super-resolution(SR)microscopy techniques have been developed to break the di®raction limit using subdi®raction excitation to spatially modulate the°uorescence emission.Photomodulatable°uorescent proteins(FPs)can be activated by light of speci¯c wavelengths to produce either stochastic or patterned subdi®raction excitation,resulting in improved optical resolution.In this review,we focus on the recently developed photomodulatable FPs or commonly used SR microscopies and discuss the concepts and strategies for optimizing and selecting the biochemical and photophysical properties of PMFPs to improve the spatiotemporal resolution of SR techniques,especially time-lapse live-cell SR techniques.
基金supported by grants from National Natural Science Foundation of China(31571033,32220103007)Major Program of the National Natural Science Foundation of China(T2293720/T2293722)Strategic Priority Research Program of Chinese Academy of Sciences(XDB37030303)。
文摘Apparently,the genomes of many organisms are pervasively transcribed,and long noncoding RNAs(lncRNAs)make up the majority of cellular transcripts.LncRNAs have been reported to play important roles in many biological processes;however,their effects on locomotion are poorly understood.Here,we presented a novel lncRNA,Locomotion Regulatory Gene(LRG),which participates in locomotion by sequestering Synaptotagmin 1(SYT1).LRG deficiency resulted in higher locomotion speed which could be rescued by pan-neuronal overexpression but not by limited ellipsoid body,motoneuron or muscle-expression of LRG.At the molecular level,the synaptic vesicles(SVs)release and movement-related SYT1 protein was recognized as LRG-interacting protein candidate.Furthermore,LRG had no effects on SYT1 expression.Genetically,the behavioral defects in LRG mutant could be rescued by pan-neuronal knock-down of Syt1.Taken together,all the results suggested LRG exerts regulatory effects on locomotion via sequestering SYT1 thereby blocking its function without affecting its expression.Our work displays a new function of lncRNA and provides insights for revealing the pathogenesis of neurological diseases with motor disorders.
