Based on the data of daily precipitation in 11 national ground meteorological observation stations in Jining City from 1981 to 2020,the interdecadal variation,intensity,range and spatial distribution of rainstorms in ...Based on the data of daily precipitation in 11 national ground meteorological observation stations in Jining City from 1981 to 2020,the interdecadal variation,intensity,range and spatial distribution of rainstorms in Jining City were analyzed.The results show that the number of rainstorm days and the total amount of rainstorms in Jining City had significant changes among different decades.There was a continuous upward trend from the 1980s to the early 21 st century and a decrease after the early 21 st century.Rainstorms had distinct seasonal characteristics.They were mainly concentrated in summer,especially in July and August.In terms of spatial distribution,the frequency and intensity of rainstorms in the southeastern regions were significantly higher than those in the northwestern regions.The above results can provide a scientific basis for flood control and disaster reduction in Jining City.展开更多
Based on the observation data of meteorological observation stations in Jining City during 1970-2024,MK mutation test and principal component analysis(PCA)were used to study the evolution characteristics of urban heat...Based on the observation data of meteorological observation stations in Jining City during 1970-2024,MK mutation test and principal component analysis(PCA)were used to study the evolution characteristics of urban heat island intensity(UHII)and the contribution rate of various influencing factors in Jining City over the past 55 years.The results show that from 1970 to 2024,the UHII in Jining City generally rose at a rate of 0.1℃/10 a.On the interannual scale,the correlation between temperature and UHII was most significantly positive.On the seasonal scale,there was a strong negative correlation between wind speed and UHII.PCA reveals that temperature had a significant positive impact on the increase of UHII in Jining City.展开更多
[ Objective] The study aimed to discuss the characteristics of atmospheric environmental pollution in Jining City. Methed] Based on the environmental monitoring data and synchronous meteorological data in 2006 -2010 ...[ Objective] The study aimed to discuss the characteristics of atmospheric environmental pollution in Jining City. Methed] Based on the environmental monitoring data and synchronous meteorological data in 2006 -2010 in Jining City, the distribution regularity of main atmospheric pol- lutants in this city was studied by the method of statistical analysis. [ Result] There were obvious spatial and temporal distribution of atmospheric pollutants in Jining City during 2006 -2010 under the comprehensive effects of meteorological conditions and pollution source emission. That is, at- mospheric pollution was serious in winter but slight in summer, and it was severe in the morning and evening. The load coefficients of SO2, NO2 and PM10 in the atmosphere showed an increasing trend from 2006 to 2010, and their concentrations had close relations with wind direction and ve- locity, urban heat island effect, and motor vehicle quantity. [ Conclusion] This research has important practical significance to the control of atmos- pheric pollution in Jining City.展开更多
Parkinson’s disease is a common neurodegenerative disease with movement disorders associated with the intracytoplasmic deposition of aggregate proteins such asα-synuclein in neurons.As one of the major intracellular...Parkinson’s disease is a common neurodegenerative disease with movement disorders associated with the intracytoplasmic deposition of aggregate proteins such asα-synuclein in neurons.As one of the major intracellular degradation pathways,the autophagy-lysosome pathway plays an important role in eliminating these proteins.Accumulating evidence has shown that upregulation of the autophagy-lysosome pathway may contribute to the clearance ofα-synuclein aggregates and protect against degeneration of dopaminergic neurons in Parkinson’s disease.Moreover,multiple genes associated with the pathogenesis of Parkinson’s disease are intimately linked to alterations in the autophagy-lysosome pathway.Thus,this pathway appears to be a promising therapeutic target for treatment of Parkinson’s disease.In this review,we briefly introduce the machinery of autophagy.Then,we provide a description of the effects of Parkinson’s disease–related genes on the autophagy-lysosome pathway.Finally,we highlight the potential chemical and genetic therapeutic strategies targeting the autophagy–lysosome pathway and their applications in Parkinson’s disease.展开更多
BACKGROUND Middle meningeal artery embolization(MMAE)is emerging as a promising treatment for chronic subdural hematoma(CSDH),serving both as an adjunct to surgery and as a primary therapeutic option depending on pati...BACKGROUND Middle meningeal artery embolization(MMAE)is emerging as a promising treatment for chronic subdural hematoma(CSDH),serving both as an adjunct to surgery and as a primary therapeutic option depending on patient presentation.Due to its low recurrence rate and minimal complications,MMAE has gained increasing acceptance among clinicians in recent years.This report presents a case of diplopia following MMAE due to the presence of a potential anastomotic artery,aiming to enhance awareness of this complication.CASE SUMMARY A 60-year-old male patient presented with a headache following head trauma,and cranial computed tomography revealed a left-sided CSDH.The patient underwent left MMAE;however,polyvinyl alcohol particles inadvertently flowed into the lacrimal artery through an anastomotic artery,resulting in diplopia due to impaired abduction of the left eye.The diplopia resolved by postoperative day 40.The patient’s headache resolved by postoperative day 7,and the hematoma completely resolved by postoperative day 108.CONCLUSION Potential anastomotic arteries in the middle meningeal artery(MMA)can lead to serious complications.Superselective angiography of the MMA or its branches prior to embolization is essential.Performing embolization distal to potential anastomotic sites can reduce risks,and the presence of an anastomosis may warrant coil embolization or termination of the procedure.展开更多
Objective To explore the sequential effects of hypoxic exercising on miR-27/PPARγand lipid metabolism targetgene and protein expression levels in the obesity rats’liver.Methods 13-week-old male diet-induced obesity ...Objective To explore the sequential effects of hypoxic exercising on miR-27/PPARγand lipid metabolism targetgene and protein expression levels in the obesity rats’liver.Methods 13-week-old male diet-induced obesity rats were randomlydivided into three groups(n=10):normal oxygen concentration quiet group(N),hypoxia quiet group(H),hypoxic exercise group(HE).Exercise training on the horizontal animal treadmill for 1 h/d,5 d/week for a total of 4 week,and the intensity of horizontaltreadmill training was 20 m/min(hypoxic concentration was 13.6%).Comparison of the weights of perirenal fat and epididymal fat in rats across different groups and calculation of Lee’s index based on body weight and body length of rats in each group were done.And the serum concentrations of total cholesterol(TC),triglyceride(TG),low density lipoprotein cholesterol(LDL-C),high-densitylipoprotein cholesterol(HDL-C)levels were detected.RT-PCR and Western Blot were used to detect the levels of miR-27,PPARγ,CYP7A1 and CD36.Results Hypoxic exercise decreased the expression levels of miR-27 in the obese rats’liver,however,theexpression level of PPARγwas gradually increased.The expression levels of miR-27 in HE group were significantly lower than Ngroup(P<0.05).The expression levels of PPARγmRNA in N group were significantly lower than H group(P<0.05),especially lowerthan HE group(P<0.01).The protein expression of PPARγprotein in N group was significantly lower than that other groups(P<0.01).The expression of lipid metabolism-related genes and proteins increased in the obese rats’liver.The expression of CYP7A1mRNA in N group was significantly lower than H group(P<0.05),especially lower than HE group(P<0.01).The expression ofCYP7A1 protein in the obese rats’liver in N group was extremely lower than H group and HE group(P<0.01).The proteinexpression of CD36 in N group was significantly lower than that in HE group(P<0.05).Hypoxia exercise improved the relatedphysiological and biochemical indexes of lipid metabolism disorder.The perirenal fat weight of obese rats in HE group wasextremely lower than N group and H group(P<0.01),and the perirenal fat weight in N group was significantly higher than H group(P<0.05).The epididymal fat weight in N group was significantly higher than H group(P<0.05),and extremely higher than HEgroup(P<0.01).The Lee’s index in HE group was extremely lower than N group and H group(P<0.01).The serum concentration ofTC in obese rats in HE group was extremely lower than N group and H group(P<0.01).The serum concentration of TG in HE groupwas extremely lower than N group and H group(P<0.01).The serum concentration of LDL-C in N group was extremely higher thanHE group(P<0.01).The serum concentration of HDL-C in N group was extremely lower than H group(P<0.01).Conclusion Hypoxiaand hypoxia exercise may negatively regulate the levels of PPARγby inhibiting miR-27 in the obese rats’liver,thereby affecting theexpression of downstream target genes CYP7A1 and CD36,and promoting cholesterol,fatty acid oxidation and HDL-C transport inthe liver,and ultimately the lipid levels in obese rats were improved.The effect of hypoxia exercise on improving blood lipid isbetter than simple hypoxia intervention.展开更多
Objective:Early predicting response before neoadjuvant chemotherapy(NAC)is crucial for personalized treatment plans for locally advanced breast cancer patients.We aim to develop a multi-task model using multiscale who...Objective:Early predicting response before neoadjuvant chemotherapy(NAC)is crucial for personalized treatment plans for locally advanced breast cancer patients.We aim to develop a multi-task model using multiscale whole slide images(WSIs)features to predict the response to breast cancer NAC more finely.Methods:This work collected 1,670 whole slide images for training and validation sets,internal testing sets,external testing sets,and prospective testing sets of the weakly-supervised deep learning-based multi-task model(DLMM)in predicting treatment response and pCR to NAC.Our approach models two-by-two feature interactions across scales by employing concatenate fusion of single-scale feature representations,and controls the expressiveness of each representation via a gating-based attention mechanism.Results:In the retrospective analysis,DLMM exhibited excellent predictive performance for the prediction of treatment response,with area under the receiver operating characteristic curves(AUCs)of 0.869[95%confidence interval(95%CI):0.806−0.933]in the internal testing set and 0.841(95%CI:0.814−0.867)in the external testing sets.For the pCR prediction task,DLMM reached AUCs of 0.865(95%CI:0.763−0.964)in the internal testing and 0.821(95%CI:0.763−0.878)in the pooled external testing set.In the prospective testing study,DLMM also demonstrated favorable predictive performance,with AUCs of 0.829(95%CI:0.754−0.903)and 0.821(95%CI:0.692−0.949)in treatment response and pCR prediction,respectively.DLMM significantly outperformed the baseline models in all testing sets(P<0.05).Heatmaps were employed to interpret the decision-making basis of the model.Furthermore,it was discovered that high DLMM scores were associated with immune-related pathways and cells in the microenvironment during biological basis exploration.Conclusions:The DLMM represents a valuable tool that aids clinicians in selecting personalized treatment strategies for breast cancer patients.展开更多
The compelling case report by Xie et al,published in a renowned medical journal,is an excellent example of meticulous clinical evaluation,comprehensive labo-ratory testing,advanced imaging,and genetic analysis.The aut...The compelling case report by Xie et al,published in a renowned medical journal,is an excellent example of meticulous clinical evaluation,comprehensive labo-ratory testing,advanced imaging,and genetic analysis.The authors identified novel compound heterozygous mutations in the hemojuvelin gene of a patient diagnosed with juvenile hemochromatosis.They suggested that long-term,strategic phlebotomy might offer a novel therapeutic strategy for severe juvenile hemochromatosis,challenging the traditional treatment paradigms.展开更多
Background:VPS37A(VPS37A subunit of ESCRT-I),a component of the ESCRT-I(endosomal sorting complex required for transport I)complex,mediates vesicular trafficking through sorting endocytic ubiquitinated cargos into mul...Background:VPS37A(VPS37A subunit of ESCRT-I),a component of the ESCRT-I(endosomal sorting complex required for transport I)complex,mediates vesicular trafficking through sorting endocytic ubiquitinated cargos into multivesicular bodies(MVBs).Although accumulating evidence indicates that VPS37A deficiency occurs in numerous malignancies and exerts tumor-suppressive effects during cancer progression,its functional significance in colorectal cancer(CRC)pathogenesis remains poorly characterized.Therefore,this study aims to further investigate the functional and molecular mechanisms by which VPS37A downregulation contributes to malignant biological phenotypes in CRC,with a specific focus on how its dysregulation affects cell death pathways.Methods:Multi-omics analysis of TCGA,GEO,and CPTAC cohorts identified VPS37A as a downregulated tumor suppressor gene in CRC.The prognostic relevance of VPS37A was validated in two clinical cohorts(Cohorts 1 and 2)using immunohistochemistry.Functional assays in VPS37A-overexpressing CRC cells and xenografts assessed proliferation,cell cycle progression,and stress-induced cell death.RNA sequencing,nuclear factor kappa-B(NF-κB)luciferase reporter assays,and lysosomal inhibition experiments elucidated the mechanisms underlying tumor necrosis factor receptor 1(TNFR1)degradation.Results:VPS37A is significantly downregulated in advanced-stage CRC and independently predicts poor survival.Functionally,VPS37A overexpression suppresses proliferation and induces G2/M arrest in vitro,while reducing xenograft growth.Under metabolic stress(glucose deprivation/galactose adaptation),VPS37A triggers cell death via apoptosis,necroptosis,and ferroptosis.Mechanistically,VPS37A redirects TNFR1 to lysosomal degradation,suppressing NF-κB nuclear translocation and transcriptional activity.Conclusion:VPS37A deficiency drives CRC progression by sustaining TNFR1/NF-κB signaling under metabolic stress.Restoring VPS37A activity promotes TNFR1 degradation,offering a therapeutic strategy to counteract NF-κB-mediated treatment resistance in CRC.展开更多
BACKGROUND The pathophysiology of diabetic kidney disease(DKD)is complex.Interfering with the processes of pyroptosis and fibrosis is an effective strategy for slowing DKD progression.Previous studies have revealed th...BACKGROUND The pathophysiology of diabetic kidney disease(DKD)is complex.Interfering with the processes of pyroptosis and fibrosis is an effective strategy for slowing DKD progression.Previous studies have revealed that nuclear receptor subfamily 4 group A member 1(NR4A1)may serve as a novel pathogenic element in DKD;however,the specific mechanism by which it contributes to pyroptosis and fibrosis in DKD is unknown.AIM To investigate the role of NR4A1 in renal pyroptosis and fibrosis in DKD and possible molecular mechanisms.METHODS Streptozotocin 60 mg/kg was injected intraperitoneally to establish a rat model of DKD.Typically,45 mmol/L glucose[high glucose(HG)]was used to activate HK-2 cells to mimic the DKD model in vitro.HK-2 cells were transfected with NR4A1 siRNA to silence NR4A1.RESULTS NR4A1 was elevated in renal tissues of DKD rats and HG-stimulated HK-2 cells.Concurrently,NOD-like receptor protein 3(NLRP3)and phosphoinositide 3-kinase(PI3K)/protein kinase B(AKT)pathways were triggered,and pyroptosis and expression of fibrosis-linked elements was increased in vivo and in vitro.These alterations were significantly reversed via NR4A1 silencing.CONCLUSION Inhibition of NR4A1 mitigated pyroptosis and fibrosis via suppressing NLRP3 activation and the PI3K/AKT pathway in HG-activated HK-2 cells.展开更多
Background:It is widely accepted that an alteration in lipid metabolism can serve as an essential hallmark for the growth and progression of various malignancies.However,the potential of lipid metabolism genes(LMGs)an...Background:It is widely accepted that an alteration in lipid metabolism can serve as an essential hallmark for the growth and progression of various malignancies.However,the potential of lipid metabolism genes(LMGs)and related pathways as drug targets for rectal adenocarcinoma(READ)treatment and prognostic evaluation requires further investigation.The objective of the present study was to construct a prognostic prediction model for READ based on LMGs,and to evaluate its performance.Methods:The RNA-seq FPKM data and clinical data from READ patients and normal rectal tissues were downloaded from The Cancer Genome Atlas database.The LMGs were acquired from the Molecular Signatures Database.Subsequently,LMGs associated with the prognosis of READ were screened,and these genes were used to construct a prognostic prediction model for READ patients.The model was then subjected to rigorous evaluation.Furthermore,immune infiltration and limma analyses were performed in different clusters,and pathway enrichment analyses were performed on the screened differentially expressed genes(DEGs).Results:A prognostic prediction model was constructed based on 5 LMGs(PLA2G2F,CBR1,CHKB,ETNPPL,and ARV1)to classify READ into high-and low-risk groups,with the low-risk group exhibiting a higher survival rate.Consequently,the prognostic model demonstrated notable predictive capability.A total of 139 DEGs were identified,38 of which were identified as being upregulated and 101 as being downregulated.Furthermore,a comparison of the immune microenvironment and immune pathways between the low-and high-risk groups revealed significant disparities.Conclusions:Predictive models based on 5 lipid metabolism DEGs effectively predicted the prognosis of READ patients.Moreover,these 5 LMGs have potential as molecular biomarkers and therapeutic targets for READ patients.展开更多
CD8^(+)T cell exhaustion,a critical challenge in the immune response to cancer,is characterized by a profound decline in the functionality of effector CD8^(+)T cells.This state of exhaustion is accompanied by the upre...CD8^(+)T cell exhaustion,a critical challenge in the immune response to cancer,is characterized by a profound decline in the functionality of effector CD8^(+)T cells.This state of exhaustion is accompanied by the upregulation of various inhibitory receptors and significant shifts in both transcriptional and epigenetic profiles,thus ultimately leading to inadequate tumor control.Therapeutic strategies aimed at reversing CD8^(+)T cell exhaustion have the potential to rejuvenate immune responses and enhance treatment efficacy.This review compiles current knowledge regarding the molecular mechanisms underlying CD8^(+)T cell exhaustion,including the roles of immune checkpoint molecules,the tumor microenvironment,metabolic reprogramming,transcription factors,and epigenetic modifications.Emerging therapeutic approaches designed to combat CD8^(+)T cell exhaustion are evaluated,with emphasis on the modulation of immune checkpoints;targeting of metabolic and transcriptional changes;and exploration of other innovative strategies,such as epigenetic editing and engineered CAR-T cells.Importantly,we expand the exhaustion concept to immune cells beyond CD8^(+)T cells,such as CD4^(+)T cells,natural killer cells,and myeloid populations,thereby highlighting the broader implications of systemic immunosuppression in the cancer context.Finally,we propose avenues for future research aimed at further elucidating the factors and molecular mechanisms associated with CD8^(+)T cell exhaustion,thereby underscoring the critical need for strategies aimed at reversing this state to improve outcomes in cancer immunotherapy.展开更多
BACKGROUND Squamous cell carcinoma(SCC)of the colon is a rare malignant tumor with an unclear pathogenesis.Its clinical presentation is similar to that of adenocarcinoma,and there are no standard treatment guidelines....BACKGROUND Squamous cell carcinoma(SCC)of the colon is a rare malignant tumor with an unclear pathogenesis.Its clinical presentation is similar to that of adenocarcinoma,and there are no standard treatment guidelines.Treatment for SCC of the colon is mainly based on the protocols for colon adenocarcinoma.In advanced stages,colon SCC is highly invasive,prone to distant metastasis,and has a worse prog-nosis than adenocarcinoma.Furthermore,pancreatic metastasis from colon SCC is even rarer.CASE SUMMARY The patient presented with abdominal pain and was diagnosed with SCC of the descending colon following colonoscopy.Preoperative examinations did not reveal any obvious metastasis to other organs,and the patient underwent laparo-scopic radical resection of the descending colon cancer.During surgery,suspi-cious metastases to the pancreatic body and tail,splenic vessels,and splenic hilum were found,leading to combined resection of the pancreatic body and tail along with the spleen.Postoperative pathology confirmed moderately to poorly diffe-rentiated SCC with nerve invasion.The patient developed postoperative compli-cations,including abdominal cavity infection,acute myocardial infarction,and deep vein thrombosis in the lower limbs.Despite active symptomatic treatment and stabilization of the patient’s vital signs,the patient did not undergo adjuvant chemotherapy due to an Eastern Cooperative Oncology Group score of 3.The patient passed away 3 months postoperatively due to multiple organ failure.CONCLUSION This case highlights the aggressive nature of colorectal SCC with atypical meta-stasis and underscores the necessity for multidisciplinary perioperative management.展开更多
BACKGROUND Lung cancer is the most prevalent malignant tumor in human body,and is characterized by a high level of malignancy.The most common metastatic sites include the liver,bone,brain,and adrenal gland,while lung ...BACKGROUND Lung cancer is the most prevalent malignant tumor in human body,and is characterized by a high level of malignancy.The most common metastatic sites include the liver,bone,brain,and adrenal gland,while lung cancer resulting in gastrointestinal tract metastasis is uncommon.CASE SUMMARY A 74-years-old man with lung cancer was hospitalized owing to blood in the stool,The cause was identified as metastasis to the small intestine,and the patient subsequently underwent radical resection of the small intestine tumor.Currently,the overall condition of the patient is good,and undergoing combined chemotherapy.CONCLUSION Early intervention in patients with metastatic tumors can significantly improve prognosis.展开更多
Chen et al demonstrated that regulator of G protein signaling(RGS)4 promotes gastric cancer(GC)progression by activating the focal adhesion kinase/phospha-tidyl-inositol-3-kinase/protein kinase B pathway and inducing ...Chen et al demonstrated that regulator of G protein signaling(RGS)4 promotes gastric cancer(GC)progression by activating the focal adhesion kinase/phospha-tidyl-inositol-3-kinase/protein kinase B pathway and inducing epithelial-mesen-chymal transition.Although their multilevel approach integrating clinical data,functional assays,and xenograft models demonstrated a key role for RGS4 in GC pathogenesis,several limitations should be considered.The mechanism of the RGS4-focal adhesion kinase interaction remains unclear,specifically whether it involves direct binding or intermediaries.The clinical analysis of 90 patients lacks stratification by GC subtypes or immune features,potentially limiting generaliz-ability.Furthermore,fully validating RGS4’s oncogenic role requires additional studies,including functional assays in chemotherapy-resistant and metastatic cell lines,metastasis models including orthotopic implantation and tail vein injection,and comparison with other RGS family members.Addressing these via targeted mechanistic studies and expanded clinical validation could strengthen RGS4’s po-tential as a therapeutic target in GC.展开更多
As living conditions improve and diagnostic capabilities advance,the incidence of tumors has increased,with cancer becoming a leading cause of death worldwide.Surgery,chemotherapy,and radiotherapy are the most common ...As living conditions improve and diagnostic capabilities advance,the incidence of tumors has increased,with cancer becoming a leading cause of death worldwide.Surgery,chemotherapy,and radiotherapy are the most common treatments.Despite advances in treatment options,chemotherapy remains a routine first-line treatment for most tumors.Due to the continuous and extensive use of chemotherapy drugs,tumor resistance often develops,becoming a significant cause of treatment failure and poor prognosis.Recent research has increasingly focused on how long stranded noncoding RNAs(LncRNAs)influence the development of malignant tumors and drug resistance by regulating gene expression and other biological mechanisms during cell growth.Studies have demonstrated that variations in lncRNA expression levels,influenced by both interpatient variability and intratumoral genetic and epigenetic differences,are closely linked to tumor drug resistance.Therefore,this review advocates using lncRNA as a framework to investigate the regulation of genes associated with drug resistance,proposing lncRNA-targeted therapeutic strategies to potentially increase the efficacy of chemotherapy,improve patient outcomes,and guide future research directions.展开更多
This article summarizes recent advances in the understanding of RNA-binding proteins(RBPs),with a focus on their roles in exercise-induced mRNA regulation and their implications for schizophrenia(SZ).RBPs are critical...This article summarizes recent advances in the understanding of RNA-binding proteins(RBPs),with a focus on their roles in exercise-induced mRNA regulation and their implications for schizophrenia(SZ).RBPs are critical regulators of mRNA stability,splicing,transport,translation,and degradation,directly influencing gene expression through sequence-and structure-specific binding.In the nervous system,RBPs sustain synaptic plasticity,neural development,and neuronal homeostasis.Emerging evidence shows that exercise modulates the expression and activity of RBPs,thereby influencing mRNA translation and neurotransmitter signaling,which may underlie its beneficial effects on brain function.Dysregulation of specific RBPs has been identified in SZ,implicating them in disrupted synaptic transmission,impaired plasticity,and neuroinflammation.RBPs involved in memory and emotional regulation show marked dysfunction in SZ patients.Some RBPs have been proposed as potential biomarkers for early diagnosis and treatment monitoring.Moreover,therapeutic modulation of RBPs,through pharmacological or behavioral interventions such as exercise,may restore neuronal function by targeting post-transcriptional gene regulation.Exercise,as a non-invasive modulator of RBP expression,holds promise as an adjunctive strategy in SZ treatment,particularly in early stages.Further research into RBP-mediated pathways may offer novel insights into SZ pathophysiology and inform the development of targeted interventions.展开更多
Strontium has similar chemical properties to calcium and has recently been recognized as a non-essential beneficial element for plants.In order to compare the effects of strontium and calcium on improving salt toleran...Strontium has similar chemical properties to calcium and has recently been recognized as a non-essential beneficial element for plants.In order to compare the effects of strontium and calcium on improving salt tolerance of Chinese cabbage during the germination stage,2,4,and 8 mmol/L of SrCl_(2),CaCl_(2) or an equimolar mixture of both were added separately to a 150 mmol/L NaCl solution.The results showed that Ca-Sr addition significantly increased seed viability,seed vigor,seed germination rate and seed germination uniformity of Chinese cabbage compared with the salt-control group.The differences in germination percentage(GP)and germination energy(GE)among the Ca-addition and Sr-addition groups were not significant,and the differences in coefficient of rate of germination(CRG),index of rate of germination(IRG)and coefficient of variation of the germination time(CVT)were relatively small,but clear differences were observed in germination index(GI),vigor index(VI)and coefficient of uniformity of germination(CUG).The results of GI and VI indicated that the higher the concentration of Ca-addition or Sr-addition,the more significant the enhancement of seed vigor.Under saline stress(150 mmol/L NaCl),the Ca-Sr co-addition outperformed Sr-treatment alone,and Ca-addition achieved the highest seed vigor at equivalent concentrations.Furthermore,all Ca-Sr treatments significantly enhanced the uniformity of Chinese cabbage sprouts exposed to 150 mM NaCl,with the best uniformity improved by the addition of 2 and 4 mmol/L SrCl_(2).Ca-Sr treatments increased the salt tolerance of Chinese cabbage sprouts during the germination stage mainly because the Ca2+and Sr2+significantly enhanced plasma membrane stability and reduced oxidative stress(as indicated by decreased contents of malondialdehyde and O2⋅-contents)in sprouts.The decrease of soluble sugar and proline content caused by Ca-Sr addition implies that elevated levels of these osmolytes were not the primary contributors to improved seed germinability in Chinese cabbage.These findings demonstrate that Sr is a beneficial element for enhancing salt tolerance in plants,laying a theoretical foundation for the development and application of strontium in agriculture.展开更多
Alcohol use has contributed to large disease burdens,and alcohol-related problems are prevalent among patients in general hospitals and primary care settings.This review aims to deepen the understanding of screening,i...Alcohol use has contributed to large disease burdens,and alcohol-related problems are prevalent among patients in general hospitals and primary care settings.This review aims to deepen the understanding of screening,intervention,and treatment referral for alcohol-related problems in these settings.We searched the literature published in English from PubMed,ScienceDirect,and the World Health Organization website.We found that while a series of screening tools can be used,screening rates were low.The awareness of screening should be strengthened.Brief interventions and pharmacotherapy are the two main methods used in general hospitals and primary care settings,with complementary and alternative medicine considered under the framework of integrative medicine.Individuals with severe alcohol-related problems or alcohol use disorders should be referred to specialty treatment for alcohol abuse,but referral is sometimes ignored.A gap exists between general hospitals/primary care facilities and specialized alcohol-related treatment providers.In conclusion,screening,intervention,and referral to treatment comprise a continuum for patients with alcohol-related problems.Opportunities to prevent and manage these problems in general hospitals and primary care settings are abundant,and taking action will promote the addressing of this public health issue.展开更多
The rapid advancement of technology and the increasing speed of vehicles have led to a substantial rise in energy consumption and growing concern over environmental pollution.Beyond the promotion of new energy vehicle...The rapid advancement of technology and the increasing speed of vehicles have led to a substantial rise in energy consumption and growing concern over environmental pollution.Beyond the promotion of new energy vehicles,reducing aerodynamic drag remains a critical strategy for improving energy efficiency and lowering emissions.This study investigates the influence of key geometric parameters on the aerodynamic drag of vehicles.A parametric vehicle model was developed,and computational fluid dynamics(CFD)simulations were conducted to analyse variations in the drag coefficient(C_(d))and pressure distribution across different design configurations.The results reveal that the optimal aerodynamic performance—characterized by a minimized drag coefficient—is achieved with the following parameter settings:engine hood angle(α)of 15°,windshield angle(β)of 25°,rear window angle(γ)of 40°,rear upwards tail lift angle(θ)of 10°,ground clearance(d)of 100 mm,and side edge angle(s)of 5°.These findings offer valuable guidance for the aerodynamic optimization of vehicle body design and contribute to strategies aimed at energy conservation and emission reduction in the automotive sector.展开更多
基金the Project of Jining Meteorological Bureau(2023JNZL09).
文摘Based on the data of daily precipitation in 11 national ground meteorological observation stations in Jining City from 1981 to 2020,the interdecadal variation,intensity,range and spatial distribution of rainstorms in Jining City were analyzed.The results show that the number of rainstorm days and the total amount of rainstorms in Jining City had significant changes among different decades.There was a continuous upward trend from the 1980s to the early 21 st century and a decrease after the early 21 st century.Rainstorms had distinct seasonal characteristics.They were mainly concentrated in summer,especially in July and August.In terms of spatial distribution,the frequency and intensity of rainstorms in the southeastern regions were significantly higher than those in the northwestern regions.The above results can provide a scientific basis for flood control and disaster reduction in Jining City.
基金Supported by the Project of Jining Meteorological Bureau(2024JNZL08).
文摘Based on the observation data of meteorological observation stations in Jining City during 1970-2024,MK mutation test and principal component analysis(PCA)were used to study the evolution characteristics of urban heat island intensity(UHII)and the contribution rate of various influencing factors in Jining City over the past 55 years.The results show that from 1970 to 2024,the UHII in Jining City generally rose at a rate of 0.1℃/10 a.On the interannual scale,the correlation between temperature and UHII was most significantly positive.On the seasonal scale,there was a strong negative correlation between wind speed and UHII.PCA reveals that temperature had a significant positive impact on the increase of UHII in Jining City.
文摘[ Objective] The study aimed to discuss the characteristics of atmospheric environmental pollution in Jining City. Methed] Based on the environmental monitoring data and synchronous meteorological data in 2006 -2010 in Jining City, the distribution regularity of main atmospheric pol- lutants in this city was studied by the method of statistical analysis. [ Result] There were obvious spatial and temporal distribution of atmospheric pollutants in Jining City during 2006 -2010 under the comprehensive effects of meteorological conditions and pollution source emission. That is, at- mospheric pollution was serious in winter but slight in summer, and it was severe in the morning and evening. The load coefficients of SO2, NO2 and PM10 in the atmosphere showed an increasing trend from 2006 to 2010, and their concentrations had close relations with wind direction and ve- locity, urban heat island effect, and motor vehicle quantity. [ Conclusion] This research has important practical significance to the control of atmos- pheric pollution in Jining City.
基金supported by the National Natural Science Foundation of China,No.82101340(to FJ).
文摘Parkinson’s disease is a common neurodegenerative disease with movement disorders associated with the intracytoplasmic deposition of aggregate proteins such asα-synuclein in neurons.As one of the major intracellular degradation pathways,the autophagy-lysosome pathway plays an important role in eliminating these proteins.Accumulating evidence has shown that upregulation of the autophagy-lysosome pathway may contribute to the clearance ofα-synuclein aggregates and protect against degeneration of dopaminergic neurons in Parkinson’s disease.Moreover,multiple genes associated with the pathogenesis of Parkinson’s disease are intimately linked to alterations in the autophagy-lysosome pathway.Thus,this pathway appears to be a promising therapeutic target for treatment of Parkinson’s disease.In this review,we briefly introduce the machinery of autophagy.Then,we provide a description of the effects of Parkinson’s disease–related genes on the autophagy-lysosome pathway.Finally,we highlight the potential chemical and genetic therapeutic strategies targeting the autophagy–lysosome pathway and their applications in Parkinson’s disease.
文摘BACKGROUND Middle meningeal artery embolization(MMAE)is emerging as a promising treatment for chronic subdural hematoma(CSDH),serving both as an adjunct to surgery and as a primary therapeutic option depending on patient presentation.Due to its low recurrence rate and minimal complications,MMAE has gained increasing acceptance among clinicians in recent years.This report presents a case of diplopia following MMAE due to the presence of a potential anastomotic artery,aiming to enhance awareness of this complication.CASE SUMMARY A 60-year-old male patient presented with a headache following head trauma,and cranial computed tomography revealed a left-sided CSDH.The patient underwent left MMAE;however,polyvinyl alcohol particles inadvertently flowed into the lacrimal artery through an anastomotic artery,resulting in diplopia due to impaired abduction of the left eye.The diplopia resolved by postoperative day 40.The patient’s headache resolved by postoperative day 7,and the hematoma completely resolved by postoperative day 108.CONCLUSION Potential anastomotic arteries in the middle meningeal artery(MMA)can lead to serious complications.Superselective angiography of the MMA or its branches prior to embolization is essential.Performing embolization distal to potential anastomotic sites can reduce risks,and the presence of an anastomosis may warrant coil embolization or termination of the procedure.
文摘Objective To explore the sequential effects of hypoxic exercising on miR-27/PPARγand lipid metabolism targetgene and protein expression levels in the obesity rats’liver.Methods 13-week-old male diet-induced obesity rats were randomlydivided into three groups(n=10):normal oxygen concentration quiet group(N),hypoxia quiet group(H),hypoxic exercise group(HE).Exercise training on the horizontal animal treadmill for 1 h/d,5 d/week for a total of 4 week,and the intensity of horizontaltreadmill training was 20 m/min(hypoxic concentration was 13.6%).Comparison of the weights of perirenal fat and epididymal fat in rats across different groups and calculation of Lee’s index based on body weight and body length of rats in each group were done.And the serum concentrations of total cholesterol(TC),triglyceride(TG),low density lipoprotein cholesterol(LDL-C),high-densitylipoprotein cholesterol(HDL-C)levels were detected.RT-PCR and Western Blot were used to detect the levels of miR-27,PPARγ,CYP7A1 and CD36.Results Hypoxic exercise decreased the expression levels of miR-27 in the obese rats’liver,however,theexpression level of PPARγwas gradually increased.The expression levels of miR-27 in HE group were significantly lower than Ngroup(P<0.05).The expression levels of PPARγmRNA in N group were significantly lower than H group(P<0.05),especially lowerthan HE group(P<0.01).The protein expression of PPARγprotein in N group was significantly lower than that other groups(P<0.01).The expression of lipid metabolism-related genes and proteins increased in the obese rats’liver.The expression of CYP7A1mRNA in N group was significantly lower than H group(P<0.05),especially lower than HE group(P<0.01).The expression ofCYP7A1 protein in the obese rats’liver in N group was extremely lower than H group and HE group(P<0.01).The proteinexpression of CD36 in N group was significantly lower than that in HE group(P<0.05).Hypoxia exercise improved the relatedphysiological and biochemical indexes of lipid metabolism disorder.The perirenal fat weight of obese rats in HE group wasextremely lower than N group and H group(P<0.01),and the perirenal fat weight in N group was significantly higher than H group(P<0.05).The epididymal fat weight in N group was significantly higher than H group(P<0.05),and extremely higher than HEgroup(P<0.01).The Lee’s index in HE group was extremely lower than N group and H group(P<0.01).The serum concentration ofTC in obese rats in HE group was extremely lower than N group and H group(P<0.01).The serum concentration of TG in HE groupwas extremely lower than N group and H group(P<0.01).The serum concentration of LDL-C in N group was extremely higher thanHE group(P<0.01).The serum concentration of HDL-C in N group was extremely lower than H group(P<0.01).Conclusion Hypoxiaand hypoxia exercise may negatively regulate the levels of PPARγby inhibiting miR-27 in the obese rats’liver,thereby affecting theexpression of downstream target genes CYP7A1 and CD36,and promoting cholesterol,fatty acid oxidation and HDL-C transport inthe liver,and ultimately the lipid levels in obese rats were improved.The effect of hypoxia exercise on improving blood lipid isbetter than simple hypoxia intervention.
基金supported by the National Natural Science Foundation of China(No.82371933)the National Natural Science Foundation of Shandong Province of China(No.ZR2021MH120)+1 种基金the Taishan Scholars Project(No.tsqn202211378)the Shandong Provincial Natural Science Foundation for Excellent Young Scholars(No.ZR2024YQ075).
文摘Objective:Early predicting response before neoadjuvant chemotherapy(NAC)is crucial for personalized treatment plans for locally advanced breast cancer patients.We aim to develop a multi-task model using multiscale whole slide images(WSIs)features to predict the response to breast cancer NAC more finely.Methods:This work collected 1,670 whole slide images for training and validation sets,internal testing sets,external testing sets,and prospective testing sets of the weakly-supervised deep learning-based multi-task model(DLMM)in predicting treatment response and pCR to NAC.Our approach models two-by-two feature interactions across scales by employing concatenate fusion of single-scale feature representations,and controls the expressiveness of each representation via a gating-based attention mechanism.Results:In the retrospective analysis,DLMM exhibited excellent predictive performance for the prediction of treatment response,with area under the receiver operating characteristic curves(AUCs)of 0.869[95%confidence interval(95%CI):0.806−0.933]in the internal testing set and 0.841(95%CI:0.814−0.867)in the external testing sets.For the pCR prediction task,DLMM reached AUCs of 0.865(95%CI:0.763−0.964)in the internal testing and 0.821(95%CI:0.763−0.878)in the pooled external testing set.In the prospective testing study,DLMM also demonstrated favorable predictive performance,with AUCs of 0.829(95%CI:0.754−0.903)and 0.821(95%CI:0.692−0.949)in treatment response and pCR prediction,respectively.DLMM significantly outperformed the baseline models in all testing sets(P<0.05).Heatmaps were employed to interpret the decision-making basis of the model.Furthermore,it was discovered that high DLMM scores were associated with immune-related pathways and cells in the microenvironment during biological basis exploration.Conclusions:The DLMM represents a valuable tool that aids clinicians in selecting personalized treatment strategies for breast cancer patients.
文摘The compelling case report by Xie et al,published in a renowned medical journal,is an excellent example of meticulous clinical evaluation,comprehensive labo-ratory testing,advanced imaging,and genetic analysis.The authors identified novel compound heterozygous mutations in the hemojuvelin gene of a patient diagnosed with juvenile hemochromatosis.They suggested that long-term,strategic phlebotomy might offer a novel therapeutic strategy for severe juvenile hemochromatosis,challenging the traditional treatment paradigms.
基金funded by the National Natural Science Foundation of China(81902815,81802786)the Natural Science Foundation of Shandong Province(ZR2023MH011,ZR2019BH044,ZR2018BH025).
文摘Background:VPS37A(VPS37A subunit of ESCRT-I),a component of the ESCRT-I(endosomal sorting complex required for transport I)complex,mediates vesicular trafficking through sorting endocytic ubiquitinated cargos into multivesicular bodies(MVBs).Although accumulating evidence indicates that VPS37A deficiency occurs in numerous malignancies and exerts tumor-suppressive effects during cancer progression,its functional significance in colorectal cancer(CRC)pathogenesis remains poorly characterized.Therefore,this study aims to further investigate the functional and molecular mechanisms by which VPS37A downregulation contributes to malignant biological phenotypes in CRC,with a specific focus on how its dysregulation affects cell death pathways.Methods:Multi-omics analysis of TCGA,GEO,and CPTAC cohorts identified VPS37A as a downregulated tumor suppressor gene in CRC.The prognostic relevance of VPS37A was validated in two clinical cohorts(Cohorts 1 and 2)using immunohistochemistry.Functional assays in VPS37A-overexpressing CRC cells and xenografts assessed proliferation,cell cycle progression,and stress-induced cell death.RNA sequencing,nuclear factor kappa-B(NF-κB)luciferase reporter assays,and lysosomal inhibition experiments elucidated the mechanisms underlying tumor necrosis factor receptor 1(TNFR1)degradation.Results:VPS37A is significantly downregulated in advanced-stage CRC and independently predicts poor survival.Functionally,VPS37A overexpression suppresses proliferation and induces G2/M arrest in vitro,while reducing xenograft growth.Under metabolic stress(glucose deprivation/galactose adaptation),VPS37A triggers cell death via apoptosis,necroptosis,and ferroptosis.Mechanistically,VPS37A redirects TNFR1 to lysosomal degradation,suppressing NF-κB nuclear translocation and transcriptional activity.Conclusion:VPS37A deficiency drives CRC progression by sustaining TNFR1/NF-κB signaling under metabolic stress.Restoring VPS37A activity promotes TNFR1 degradation,offering a therapeutic strategy to counteract NF-κB-mediated treatment resistance in CRC.
基金Supported by Research Fund for Academician Lin He New Medicine,No.JYHL2022FMS02.
文摘BACKGROUND The pathophysiology of diabetic kidney disease(DKD)is complex.Interfering with the processes of pyroptosis and fibrosis is an effective strategy for slowing DKD progression.Previous studies have revealed that nuclear receptor subfamily 4 group A member 1(NR4A1)may serve as a novel pathogenic element in DKD;however,the specific mechanism by which it contributes to pyroptosis and fibrosis in DKD is unknown.AIM To investigate the role of NR4A1 in renal pyroptosis and fibrosis in DKD and possible molecular mechanisms.METHODS Streptozotocin 60 mg/kg was injected intraperitoneally to establish a rat model of DKD.Typically,45 mmol/L glucose[high glucose(HG)]was used to activate HK-2 cells to mimic the DKD model in vitro.HK-2 cells were transfected with NR4A1 siRNA to silence NR4A1.RESULTS NR4A1 was elevated in renal tissues of DKD rats and HG-stimulated HK-2 cells.Concurrently,NOD-like receptor protein 3(NLRP3)and phosphoinositide 3-kinase(PI3K)/protein kinase B(AKT)pathways were triggered,and pyroptosis and expression of fibrosis-linked elements was increased in vivo and in vitro.These alterations were significantly reversed via NR4A1 silencing.CONCLUSION Inhibition of NR4A1 mitigated pyroptosis and fibrosis via suppressing NLRP3 activation and the PI3K/AKT pathway in HG-activated HK-2 cells.
基金supported by the Science and Technology Research Project of the Shandong Geriatrics Society in 2024(No.LKJGG2024W072).
文摘Background:It is widely accepted that an alteration in lipid metabolism can serve as an essential hallmark for the growth and progression of various malignancies.However,the potential of lipid metabolism genes(LMGs)and related pathways as drug targets for rectal adenocarcinoma(READ)treatment and prognostic evaluation requires further investigation.The objective of the present study was to construct a prognostic prediction model for READ based on LMGs,and to evaluate its performance.Methods:The RNA-seq FPKM data and clinical data from READ patients and normal rectal tissues were downloaded from The Cancer Genome Atlas database.The LMGs were acquired from the Molecular Signatures Database.Subsequently,LMGs associated with the prognosis of READ were screened,and these genes were used to construct a prognostic prediction model for READ patients.The model was then subjected to rigorous evaluation.Furthermore,immune infiltration and limma analyses were performed in different clusters,and pathway enrichment analyses were performed on the screened differentially expressed genes(DEGs).Results:A prognostic prediction model was constructed based on 5 LMGs(PLA2G2F,CBR1,CHKB,ETNPPL,and ARV1)to classify READ into high-and low-risk groups,with the low-risk group exhibiting a higher survival rate.Consequently,the prognostic model demonstrated notable predictive capability.A total of 139 DEGs were identified,38 of which were identified as being upregulated and 101 as being downregulated.Furthermore,a comparison of the immune microenvironment and immune pathways between the low-and high-risk groups revealed significant disparities.Conclusions:Predictive models based on 5 lipid metabolism DEGs effectively predicted the prognosis of READ patients.Moreover,these 5 LMGs have potential as molecular biomarkers and therapeutic targets for READ patients.
基金supported by grants from the National Natural Science Foundation of China(Grant No.82171810)the Program of Shandong Provincial Scientific and Technological Development of Traditional Chinese Medicine(Grant No.M-2023210)。
文摘CD8^(+)T cell exhaustion,a critical challenge in the immune response to cancer,is characterized by a profound decline in the functionality of effector CD8^(+)T cells.This state of exhaustion is accompanied by the upregulation of various inhibitory receptors and significant shifts in both transcriptional and epigenetic profiles,thus ultimately leading to inadequate tumor control.Therapeutic strategies aimed at reversing CD8^(+)T cell exhaustion have the potential to rejuvenate immune responses and enhance treatment efficacy.This review compiles current knowledge regarding the molecular mechanisms underlying CD8^(+)T cell exhaustion,including the roles of immune checkpoint molecules,the tumor microenvironment,metabolic reprogramming,transcription factors,and epigenetic modifications.Emerging therapeutic approaches designed to combat CD8^(+)T cell exhaustion are evaluated,with emphasis on the modulation of immune checkpoints;targeting of metabolic and transcriptional changes;and exploration of other innovative strategies,such as epigenetic editing and engineered CAR-T cells.Importantly,we expand the exhaustion concept to immune cells beyond CD8^(+)T cells,such as CD4^(+)T cells,natural killer cells,and myeloid populations,thereby highlighting the broader implications of systemic immunosuppression in the cancer context.Finally,we propose avenues for future research aimed at further elucidating the factors and molecular mechanisms associated with CD8^(+)T cell exhaustion,thereby underscoring the critical need for strategies aimed at reversing this state to improve outcomes in cancer immunotherapy.
文摘BACKGROUND Squamous cell carcinoma(SCC)of the colon is a rare malignant tumor with an unclear pathogenesis.Its clinical presentation is similar to that of adenocarcinoma,and there are no standard treatment guidelines.Treatment for SCC of the colon is mainly based on the protocols for colon adenocarcinoma.In advanced stages,colon SCC is highly invasive,prone to distant metastasis,and has a worse prog-nosis than adenocarcinoma.Furthermore,pancreatic metastasis from colon SCC is even rarer.CASE SUMMARY The patient presented with abdominal pain and was diagnosed with SCC of the descending colon following colonoscopy.Preoperative examinations did not reveal any obvious metastasis to other organs,and the patient underwent laparo-scopic radical resection of the descending colon cancer.During surgery,suspi-cious metastases to the pancreatic body and tail,splenic vessels,and splenic hilum were found,leading to combined resection of the pancreatic body and tail along with the spleen.Postoperative pathology confirmed moderately to poorly diffe-rentiated SCC with nerve invasion.The patient developed postoperative compli-cations,including abdominal cavity infection,acute myocardial infarction,and deep vein thrombosis in the lower limbs.Despite active symptomatic treatment and stabilization of the patient’s vital signs,the patient did not undergo adjuvant chemotherapy due to an Eastern Cooperative Oncology Group score of 3.The patient passed away 3 months postoperatively due to multiple organ failure.CONCLUSION This case highlights the aggressive nature of colorectal SCC with atypical meta-stasis and underscores the necessity for multidisciplinary perioperative management.
文摘BACKGROUND Lung cancer is the most prevalent malignant tumor in human body,and is characterized by a high level of malignancy.The most common metastatic sites include the liver,bone,brain,and adrenal gland,while lung cancer resulting in gastrointestinal tract metastasis is uncommon.CASE SUMMARY A 74-years-old man with lung cancer was hospitalized owing to blood in the stool,The cause was identified as metastasis to the small intestine,and the patient subsequently underwent radical resection of the small intestine tumor.Currently,the overall condition of the patient is good,and undergoing combined chemotherapy.CONCLUSION Early intervention in patients with metastatic tumors can significantly improve prognosis.
文摘Chen et al demonstrated that regulator of G protein signaling(RGS)4 promotes gastric cancer(GC)progression by activating the focal adhesion kinase/phospha-tidyl-inositol-3-kinase/protein kinase B pathway and inducing epithelial-mesen-chymal transition.Although their multilevel approach integrating clinical data,functional assays,and xenograft models demonstrated a key role for RGS4 in GC pathogenesis,several limitations should be considered.The mechanism of the RGS4-focal adhesion kinase interaction remains unclear,specifically whether it involves direct binding or intermediaries.The clinical analysis of 90 patients lacks stratification by GC subtypes or immune features,potentially limiting generaliz-ability.Furthermore,fully validating RGS4’s oncogenic role requires additional studies,including functional assays in chemotherapy-resistant and metastatic cell lines,metastasis models including orthotopic implantation and tail vein injection,and comparison with other RGS family members.Addressing these via targeted mechanistic studies and expanded clinical validation could strengthen RGS4’s po-tential as a therapeutic target in GC.
基金supported by the grant of the Medicine and Health Care Science and Technology Development Plan Projects Foundation of Shandong Province(No.202301060260).
文摘As living conditions improve and diagnostic capabilities advance,the incidence of tumors has increased,with cancer becoming a leading cause of death worldwide.Surgery,chemotherapy,and radiotherapy are the most common treatments.Despite advances in treatment options,chemotherapy remains a routine first-line treatment for most tumors.Due to the continuous and extensive use of chemotherapy drugs,tumor resistance often develops,becoming a significant cause of treatment failure and poor prognosis.Recent research has increasingly focused on how long stranded noncoding RNAs(LncRNAs)influence the development of malignant tumors and drug resistance by regulating gene expression and other biological mechanisms during cell growth.Studies have demonstrated that variations in lncRNA expression levels,influenced by both interpatient variability and intratumoral genetic and epigenetic differences,are closely linked to tumor drug resistance.Therefore,this review advocates using lncRNA as a framework to investigate the regulation of genes associated with drug resistance,proposing lncRNA-targeted therapeutic strategies to potentially increase the efficacy of chemotherapy,improve patient outcomes,and guide future research directions.
文摘This article summarizes recent advances in the understanding of RNA-binding proteins(RBPs),with a focus on their roles in exercise-induced mRNA regulation and their implications for schizophrenia(SZ).RBPs are critical regulators of mRNA stability,splicing,transport,translation,and degradation,directly influencing gene expression through sequence-and structure-specific binding.In the nervous system,RBPs sustain synaptic plasticity,neural development,and neuronal homeostasis.Emerging evidence shows that exercise modulates the expression and activity of RBPs,thereby influencing mRNA translation and neurotransmitter signaling,which may underlie its beneficial effects on brain function.Dysregulation of specific RBPs has been identified in SZ,implicating them in disrupted synaptic transmission,impaired plasticity,and neuroinflammation.RBPs involved in memory and emotional regulation show marked dysfunction in SZ patients.Some RBPs have been proposed as potential biomarkers for early diagnosis and treatment monitoring.Moreover,therapeutic modulation of RBPs,through pharmacological or behavioral interventions such as exercise,may restore neuronal function by targeting post-transcriptional gene regulation.Exercise,as a non-invasive modulator of RBP expression,holds promise as an adjunctive strategy in SZ treatment,particularly in early stages.Further research into RBP-mediated pathways may offer novel insights into SZ pathophysiology and inform the development of targeted interventions.
基金funded by the Natural Science Foundation of Shandong Province(ZR2020MC144)the Scientific Research Training Program for Undergraduates of Qufu Normal University(XJ2024016).
文摘Strontium has similar chemical properties to calcium and has recently been recognized as a non-essential beneficial element for plants.In order to compare the effects of strontium and calcium on improving salt tolerance of Chinese cabbage during the germination stage,2,4,and 8 mmol/L of SrCl_(2),CaCl_(2) or an equimolar mixture of both were added separately to a 150 mmol/L NaCl solution.The results showed that Ca-Sr addition significantly increased seed viability,seed vigor,seed germination rate and seed germination uniformity of Chinese cabbage compared with the salt-control group.The differences in germination percentage(GP)and germination energy(GE)among the Ca-addition and Sr-addition groups were not significant,and the differences in coefficient of rate of germination(CRG),index of rate of germination(IRG)and coefficient of variation of the germination time(CVT)were relatively small,but clear differences were observed in germination index(GI),vigor index(VI)and coefficient of uniformity of germination(CUG).The results of GI and VI indicated that the higher the concentration of Ca-addition or Sr-addition,the more significant the enhancement of seed vigor.Under saline stress(150 mmol/L NaCl),the Ca-Sr co-addition outperformed Sr-treatment alone,and Ca-addition achieved the highest seed vigor at equivalent concentrations.Furthermore,all Ca-Sr treatments significantly enhanced the uniformity of Chinese cabbage sprouts exposed to 150 mM NaCl,with the best uniformity improved by the addition of 2 and 4 mmol/L SrCl_(2).Ca-Sr treatments increased the salt tolerance of Chinese cabbage sprouts during the germination stage mainly because the Ca2+and Sr2+significantly enhanced plasma membrane stability and reduced oxidative stress(as indicated by decreased contents of malondialdehyde and O2⋅-contents)in sprouts.The decrease of soluble sugar and proline content caused by Ca-Sr addition implies that elevated levels of these osmolytes were not the primary contributors to improved seed germinability in Chinese cabbage.These findings demonstrate that Sr is a beneficial element for enhancing salt tolerance in plants,laying a theoretical foundation for the development and application of strontium in agriculture.
基金Supported by Shandong Province Medical and Health Technology Development Plan Project,No.202203090753.
文摘Alcohol use has contributed to large disease burdens,and alcohol-related problems are prevalent among patients in general hospitals and primary care settings.This review aims to deepen the understanding of screening,intervention,and treatment referral for alcohol-related problems in these settings.We searched the literature published in English from PubMed,ScienceDirect,and the World Health Organization website.We found that while a series of screening tools can be used,screening rates were low.The awareness of screening should be strengthened.Brief interventions and pharmacotherapy are the two main methods used in general hospitals and primary care settings,with complementary and alternative medicine considered under the framework of integrative medicine.Individuals with severe alcohol-related problems or alcohol use disorders should be referred to specialty treatment for alcohol abuse,but referral is sometimes ignored.A gap exists between general hospitals/primary care facilities and specialized alcohol-related treatment providers.In conclusion,screening,intervention,and referral to treatment comprise a continuum for patients with alcohol-related problems.Opportunities to prevent and manage these problems in general hospitals and primary care settings are abundant,and taking action will promote the addressing of this public health issue.
基金funded by the“Hundred Outstanding Talents”Support Program of Jining University,a provincial-level key project in the field of natural sciences,grant number 2023ZYRC23Jining Key R&D Program(Soft Science)Project,No.2024JNZC010Shandong Province Key Research and Development Program(Technology-Based Small and Medium-sized Enterprises Innovation Capability Improvement)Project No.2025TSGCCZZB0679.
文摘The rapid advancement of technology and the increasing speed of vehicles have led to a substantial rise in energy consumption and growing concern over environmental pollution.Beyond the promotion of new energy vehicles,reducing aerodynamic drag remains a critical strategy for improving energy efficiency and lowering emissions.This study investigates the influence of key geometric parameters on the aerodynamic drag of vehicles.A parametric vehicle model was developed,and computational fluid dynamics(CFD)simulations were conducted to analyse variations in the drag coefficient(C_(d))and pressure distribution across different design configurations.The results reveal that the optimal aerodynamic performance—characterized by a minimized drag coefficient—is achieved with the following parameter settings:engine hood angle(α)of 15°,windshield angle(β)of 25°,rear window angle(γ)of 40°,rear upwards tail lift angle(θ)of 10°,ground clearance(d)of 100 mm,and side edge angle(s)of 5°.These findings offer valuable guidance for the aerodynamic optimization of vehicle body design and contribute to strategies aimed at energy conservation and emission reduction in the automotive sector.
