Mechanical transplanting with carpet seedlings(MC) and mechanical direct seeding(MD) are newly developed planting methods, which increase in popularity and planted area each year. Knowing the difference for yield and ...Mechanical transplanting with carpet seedlings(MC) and mechanical direct seeding(MD) are newly developed planting methods, which increase in popularity and planted area each year. Knowing the difference for yield and rice quality under different planting methods is of great importance for the development of high quality and yield cultivation techniques under mechanical conditions. Therefore, three kinds of japonica rice including hybrid japonica rice, inbreed japonica rice, and soft rice were adopted as materials. And the differences in the quality of processing, appearance, cooking and eating quality, nutrition, and the rapid viscosity analyzer(RVA) profile were studied to reveal the effects of planting methods on yield and quality of different types of japonica rice. Results showed that the milled rice and head rice rates under MC was significantly higher than those under MD, and the processing quality of inbreed japonica rice was the most stable. Compared with MC, length/width ratio of rice under MD was significantly increased, and chalkiness rate, size, and degree were significantly decreased. The protein content under MD was lower than that under MC. MC showed higher peak viscosity and breakdown value than MD. The taste value was the greatest for soft rice, followed by inbreed japonica rice, and then by japonica hybrid rice, with no significant differences resulting from planting methods. Compared with MC, MD significantly improved the appearance quality, though processing quality and nutritional quality were decreased. And there was no significant difference in cooking and eating quality between MC and MD. Under different planting methods, the appearance quality of inbreed japonica rice changed the most and the processing quality was the most stable. The nutritional, cooking and eating quality of soft rice changed the least. Therefore, according to the different planting methods and market needs, selecting the appropriate rice varieties can reduce the risks in rice production and achieve good rice quality.展开更多
In previous studies, integrative crop management (ICM) improved shoot growth and grain yield of rice (Oryza sativa L.). However, little is known about the effect of ICM on root growth and methane (CH4) emission of pad...In previous studies, integrative crop management (ICM) improved shoot growth and grain yield of rice (Oryza sativa L.). However, little is known about the effect of ICM on root growth and methane (CH4) emission of paddy rice. In this study, two rice varieties, Wuyunjing 24 and Yongyou 2640, were grown. A field experiment was conducted with three crop management treatments including zero nitrogen fertilization (0N), local farmer practice (LFP), and ICM. Root morphophysiological traits and CH4 emission from the paddy field were investigated. ICM significantly increased mean grain yield by 29.9%, with the effect attributed mainly to an increase in mean total number of spikelets by 26.4% compared to LFP. ICM increased root and shoot biomass, root length, number of roots, root oxidation activity (ROA), root bleeding rate, and root total and active absorbing surface area by respectively 24.4%, 25.7%, 17.1%, 9.3%, 18.7%, 29.5%, 12.1%, and 24.7%. The concentrations of malic, succinic, and acetic acids in root exudates were respectively 5.8%, 6.0%, and 10.5% higher in ICM than in LFP. Compared to LFP, ICM significantly decreased the rate of CH4 emission during emission peak stages and reduced total CH4 emission by 17.1%. The root morphophysiological traits were positively and significantly correlated with grain yield, whereas root length, specific root length, ROA, and root total and active absorbing surface area were negatively and significantly correlated with total CH4 emission. These results suggest that ICM could achieve the dual goals of increasing grain yield and reducing the greenhouse gas effect by improving the root morphology and physiological traits of paddy rice.展开更多
Root morphology and physiology influence aboveground growth and yield formation in rice.However,root morphological and physiological differences among rice varieties with differing nitrogen(N)sensitivities and their r...Root morphology and physiology influence aboveground growth and yield formation in rice.However,root morphological and physiological differences among rice varieties with differing nitrogen(N)sensitivities and their relationship with grain yield are still unclear.In this study,rice varieties differing in N sensitivity over many years of experiments were used.A field experiment with multiple N rates(0,90,180,270,and 360 kg ha^(-1))was conducted to elucidate the effects of N application on root morphology,root physiology,and grain yield.A pot experiment with root excision and exogenous application of 6-benzyladenine(6-BA)at heading stage was used to further verify the above effects.The findings revealed that(1)under the same N application rate,N-insensitive varieties(NIV)had relatively large root biomass(root dry weight,length,and number).Grain yield was associated with root biomass in NIV.The oxidation activity and zeatin(Z)+zeatin riboside(ZR)contents in roots obviously and positively correlated with grain yield in N-sensitive varieties(NSV),and accounted for its higher grain yield than that of NIV at lower N application rates(90 and 180 kg ha^(-1)).(2)The root dry weight required for equal grain yield of NIV was greater than that of NSV.Excision of 1/10 and 1/8 of roots at heading stage had no discernible effect on the yield of Liangyoupeijiu(NIV),and it significantly reduced yield by 11.5%and 21.3%in Tianyouhuazhan(NSV),respectively,compared to the treatment without root excision.The decrease of filled kernels and grain weight after root excision was the primary cause for the yield reduction.Root excision and exogenous 6-BA application after root excision had little influence on the root activity of NIV.The oxidation activity and Z+ZR contents in roots of NSV decreased under root excision,and the increase in the proportion of excised roots aggravated these effects.The application of exogenous 6-BA increased the root activity of NSV and increased filled kernels and grain weight,thereby reducing yield loss after root excision.Thus,the root biomass of NIV was large,and there may be a phenomenon of"root growth redundancy."Vigorous root activity was an essential feature of NSV.Selecting rice varieties with high root activity or increasing root activity by cultivation measures could lead to higher grain yield under lower N application rates.展开更多
In recent years,an increasing number of different types of japonica rice cultivars have been released in the southern rice region of China.The grain yield and quality of these new cultivars showed significant differen...In recent years,an increasing number of different types of japonica rice cultivars have been released in the southern rice region of China.The grain yield and quality of these new cultivars showed significant differences in large scale planting.However,the causes of the differences remain little known.Therefore,three typical types of japonica rice cultivars were used in this study to investigate their grain yield and quality.A scanning calorimeter(DSC),X-ray powder diffractometer(XRD),rapid viscosity analyzer(RVA)and taste analyzer were used to evaluate the cooking and eating properties.The results showed that the yield of non-soft hybrid japonica rice cultivars was significantly higher than that of non-soft inbred japonica rice cultivars and soft inbred japonica rice cultivars.Soft inbred japonica rice cultivars had a low amylose content and moderate protein content,which are the main reasons for the superior cooking and eating quality.In addition,the relative crystallinity of soft inbred japonica rice cultivars was significantly higher than that of non-soft inbred and non-soft hybrid japonica rice cultivars,which is considered the major factor resulting in higher transition temperature and gelatinization enthalpy(ΔH_(gel)).Non-soft hybrid japonica rice cultivars had a higher number of large starch granules than soft inbred and non-soft inbred japonica rice cultivars.The setback value(SB)and breakdown value(BD),indirectly reflecting the cooking and eating quality of the three types of japonica rice cultivars,also confirmed that soft inbred japonica rice cultivars with a low SB value and a high BD value had better palatability than the other two types.This study provides guidance for future plantation of different types of japonica rice cultivars in large rice-producing areas.展开更多
Reducing water consumption in rice production in China without affecting grain yield and quality is a significant challenge.This study explored how various dry cultivation methods could improve rice quality while bala...Reducing water consumption in rice production in China without affecting grain yield and quality is a significant challenge.This study explored how various dry cultivation methods could improve rice quality while balancing yield to maintain sustainable rice production.A japonica upland rice cultivar and a japonica paddy rice cultivar were cultivated in the field with three cultivation methods:plastic film mulching dry cultivation(PFMC),bare dry cultivation(BC),and continuous flooding cultivation(CF)as control.There was no significant difference in upland rice yield between PFMC and BC,nor in paddy rice yield between PFMC and CF.Compared with CF,the two varieties'yields decreased significantly with BC.Dry cultivation,especially PFMC,could decrease the active filling period,chalky rice rate,chalkiness,amylose content,gel consistency,breakdown viscosity,the ratio of glutelin to prolamin,and leaf senescence while increasing water use efficiency,protein components content,setback viscosity,grain starch branching enzyme(Q-enzyme)activity,and average filling rate.Compared with paddy rice,upland rice had a lower yield,shorter active filling period,lower chalkiness grain rate and gel consistency,higher amylose content,breakdown viscosity,protein components content,and average filling rate.Grain Q-enzyme activity and grain-filling parameters were closely related to rice quality.Reasonable dry cultivation methods could balance yield and quality,especially by improving rice's nutritional and appearance quality.展开更多
Currently,research on the co-application of straw charcoal and organic fertilizer made from livestock and poultry manure remains limited,despite their demonstrated benefits in enhancing soil fertility and improving pl...Currently,research on the co-application of straw charcoal and organic fertilizer made from livestock and poultry manure remains limited,despite their demonstrated benefits in enhancing soil fertility and improving plant physiological traits.To investigate the effects of straw charcoal on paddy soil,an experiment was conducted in fields with stable soil properties characterized by clay loam texture in the Middle-Lower Reaches of the Yangtze River,China,using the rice cultivar Nanjing 5055.展开更多
Unbalanced fertilizer application with high intensity nitrogen(N)and insufficient potassium(K)results in declining soil fertility.Balanced fertilization represents an effective approach to reduce fertilizer usage whil...Unbalanced fertilizer application with high intensity nitrogen(N)and insufficient potassium(K)results in declining soil fertility.Balanced fertilization represents an effective approach to reduce fertilizer usage while enhancing maize yield and efficiency.This study examined two N levels(180 and 225 kg N ha^(-1),abbreviated N12 and N15)and four K treatments(0,75,150,and 75+75 kg K_(2)O ha^(-1),abbreviated K0,K5,K10,and K5+5)to investigate the effects of combined N and K application on biomass,nutrient accumulation,and remobilization characteristics in waxy maize.Results indicated that grain yield increased with higher K application at constant N levels,demonstrating an average increase of 1,254.8 kg ha^(-1)(2020)and 727.3 kg ha^(-1)(2021)compared with K0.Under identical N and K applications,K5+5 enhanced grain yield through increased kernel weight.The K5+5 treatment showed no significant difference in biomass and nutrient accumulation between N12 and N15.Compared to K10,K5+5 enhanced both the average remobilization amount(RBA)of biomass and increased RBA of N,phosphorus(P)and K.Additionally,the average remobilization efficiency(RBE)of biomass,N,P,and K in K5+5 increased by 3.3,4.6,10.6,and 4.2%,respectively.Moreover,topdressing K improved the apparent contribution to grain(AC)of biomass,N,P and K,facilitating greater nutrient transfer to grains and significantly increasing nutrient harvest index.Based on yield and fertilizer use efficiency,this study recommends optimized K application(basal and topdressing 75 kg ha^(-1))and moderate reduction in N application(from 225 to 180 kg ha^(-1))for spring-sown waxy maize production in southern China.展开更多
Background Achieving a synergistic enhancement in both the yield and insect resistance of Bt cotton holds substantial importance for boosting farmers'income and ecological advantages.This study investigated the im...Background Achieving a synergistic enhancement in both the yield and insect resistance of Bt cotton holds substantial importance for boosting farmers'income and ecological advantages.This study investigated the impact of amino acid foliar fertilizer(AAF)on the yield and Cry1Ac protein(CP)content,providing valuable insights for enhancing its productivity and insect-resistance capabilities.In 2021,Sikang 1 and Sikang 3 were treated with AAF once(A1)and water(CK)during the peak flowering stage.In 2022,AAF was sprayed one(A1),two(A2),and three(A3)times,respectively,with CK serving as the control.Results Compared with the control,the A3 treatment increased seed cotton yield(SCY)by 16.0%and CP by 40.98%at 30 days after flowering.AAF application enhanced soluble protein content(SP)and glutamate pyruvate transaminase(GPT)activity,while suppressing protease and peptidase activities.Concurrently,AAF improved sucrose metabolism through elevated sucrose content and increased activities of sucrose synthase(SS)and sucrose conversion enzyme(SCE),which were also positively correlated with yield.A lower ratio of carbon-to-nitrogen(C/N)was linked to higher yields and CP content.Path analysis confirmed that SP,GPT,SS,and SCE demonstrated positive effects on CP content and SCY,respectively.Peptidase activity had negative effects on CP and SCY.The C/N ratio had negative effects on SCY and CP,respectively.Conclusions Triple foliar application of AAF maintained lower C/N ratios with enhanced carbon metabolism and protein synthesis capacity,thereby simultaneously increasing both Cry1Ac protein content and yield in Bt cotton.These findings provide critical insights for improving both pest resistance and agronomic performance in Bt cotton cultivation.展开更多
Mechanical pot-seedling transplanting is an innovatively developed transplanting method that has the potential to replace mechanical carpet-seedling transplanting. However, the initial pot-seedling transplanting machi...Mechanical pot-seedling transplanting is an innovatively developed transplanting method that has the potential to replace mechanical carpet-seedling transplanting. However, the initial pot-seedling transplanting machine lacked optimized density spacing and limited yield potential for japonica rice. Therefore, ascertaining the optimized density by wide-narrow rows and the appropriate transplanting method for yield formation and grain quality of japonica rice is of great importance for high-quality rice production. Field experiments were conducted using two japonica rice cultivars Nanjing 9108 and Nanjing 5055 under three transplanting methods in 2016 and 2017: mechanical pot-seedling transplanting with wide-narrow row(K, average row spacing of 30 cm);equidistant row(D, 33 cm×12 cm);and mechanical carpet-seedling transplanting(T, 30 cm×12.4 cm). In addition, five different density treatments were set in K(K1–K5, from 18.62×10~4 to 28.49×10~4 hills ha^(–1)). The results showed that the highest yield was produced by a planting density of 26.88×104 hills ha^(–1) in mechanical pot-seedling transplanting with wide-narrow row with a greater number of total spikelets that resulted from significantly more panicles per area and slightly more grain number per panicle, as compared with equidistant row, and yield among density in wide-narrow row showed a parabolic trend. Compared with mechanical carpet-seedling transplanting, the treatment of the highest yield increased yield significantly, which was mainly attributed to the larger sink size with improved filled-grain percentage and grain weight, higher harvest index, and increased total dry matter accumulation, especially the larger amount accumulated from heading stage to maturity stage. With the density in wide-narrow row decreasing, processing quality, appearance quality, and nutrition quality were all improved, whereas amylose content and the taste value were decreased. Compared with mechanical carpet-seedling transplanting, mechanical pot-seedling transplanting improved processing quality and nutrition quality, but decreased amylose content and deteriorated appearance quality. These results suggested that mechanical pot-seedling transplanting with wide-narrow row coupling produced a suitable planting density of 26.88×10~4 hills ha^(–1) and may be an alternative approach to improving grain yield and quality for japonica rice.展开更多
This study investigated if super rice could better cope with soil water deficit and if it could have better yield performance and water use efficiency （WUE） under alternate wetting and drying （AWD） irrigation than...This study investigated if super rice could better cope with soil water deficit and if it could have better yield performance and water use efficiency （WUE） under alternate wetting and drying （AWD） irrigation than check rice. Two super rice cultivars and two elite check rice cultivars were grown in pots with three soil moisture levels, well watered （WW）, moderate water deficit （MWD） and severe water deficit （SWD）. Two cultivars, each for super rice and check rice, were grown in field with three irrigation regimes, alternate wetting and moderate drying （AWMD）, alternate wetting and severe drying （AWSD） and conventional irrigation （CI）. Compared with that under WW, grain yield was significantly decreased under MWD and SWD treatments, with less reduction for super rice than for check rice. Super rice had higher percentage of productive tillers, deeper root distribution, higher root oxidation activity, and greater aboveground biomass production at mid and late growth stages than check rice, especially under WMD and WSD. Compared with CI,AWMD increased, whereasAWSD decreased grain yield, with more increase or less decrease for super rice than for check rice. Both MWD and SWD treatments and eitherAWMD orAWSD regime significantly increased WUE compared with WW treatment or CI regime, with more increase for super rice than for check rice. The results suggest that super rice has a stronger ability to cope with soil water deficit and holds greater promising to increase both grain yield and WUE by adoption of moderate AWD irrigation.展开更多
Although studies on the balance between yield and quality of japonica soft super rice are limited, they are crucial for super rice cultivation. In order to investigate the effects of nitrogen application rate on grain...Although studies on the balance between yield and quality of japonica soft super rice are limited, they are crucial for super rice cultivation. In order to investigate the effects of nitrogen application rate on grain yield and rice quality, two japonica soft super rice varieties, Nanjing 9108 （NJ 9108） and Nanjing 5055 （NJ 5055）, were used under seven N levels with the application rates of 0, 150, 187.5,225, 262.5, 300, and 337.5 kg ha^-1. With the increasing nitrogen application level, grain yield of both varieties first increased and then decreased. The highest yield was obtained at 300 kg ha^-1. The milling quality and protein content increased, while the appearance quality, amylose content, gel consistency, cooking/eating quality, and rice flour viscosity decreased. Milling was significantly negatively related with the eating/cooking quality whereas the appearance was significantly positively related with cooking/eating quality. These results suggest that nitrogen level significantly affects the yield and rice quality of japonica soft super rice. We conclude that the suitable nitrogen application rate for japonica soft super rice, NJ 9108 and NJ 5055, is 270 kg ha^-1, under which they obtain high yield as well as superior eating/cooking quality.展开更多
Indica-japonica hybrid rice(Oryza sativa L.) cultivars showed high yield potential and poor tasting quality when compared with common japonica rice cultivars. Large panicle is a prominent factor of high yield for indi...Indica-japonica hybrid rice(Oryza sativa L.) cultivars showed high yield potential and poor tasting quality when compared with common japonica rice cultivars. Large panicle is a prominent factor of high yield for indica-japonica hybrid rice cultivars, and the panicle weight varies greatly among different indica-japonica hybrid rice cultivars. It is important to research on yield and grain quality of different panicle weight indica-japonica hybrid rice cultivars. In this study, two different panicle types indica-japonica hybrid cultivars were used to research on the relation of yield and grain quality. The yields of two heavy panicle weights indica-japonica hybrid cultivars were significantly higher than that of two medium panicle weight rice cultivars. The cooking and eating quality and starch properties of different panicle type cultivars were evaluated. Yongyou 6715(medium panicle) and Yongyou 1852(heavy panicle) got the relatively higher cooking and eating quality. Rice cultivars with medium panicle weight had more large starch granules and higher relative crystallinity than cultivars with heavy panicle weight. Transition temperature and retrogradation enthalpy(ΔHret) of medium panicle type cultivars were significantly higher than that of heavy panicle type cultivars. There was no significant difference in amylose content among different panicle type cultivars. Protein content of heavy panicle type cultivar was higher than that of medium panicle type cultivar, and protein content is the main factor affect cooking and eating quality in this study. The cultivar Yongyou 6715 got the highest taste value with the lowest protein content. Thus, it is suggested that the emphasis on improving rice cooking and eating quality of indica-japonica hybrid rice cultivars is how to reduce the protein content in rice grain. According to the results of this study, medium panicle type with high grain weight is the desired panicle type for high quality indica-japonica hybrid rice breeding.展开更多
There is limited information about the influence of slow or controlled release fertilizer（S/CRF） on rice yield and quality. In this study, japonica rice cultivar Nanjing 9108 was used to study the effects of three d...There is limited information about the influence of slow or controlled release fertilizer（S/CRF） on rice yield and quality. In this study, japonica rice cultivar Nanjing 9108 was used to study the effects of three different S/CRFs（polymer-coated urea（PCU）, sulfur-coated urea（SCU）, and urea formaldehyde（UF）） and two fertilization modes（both S/CRF and common urea（CU） as basal fertilizer, S/CRF as basal and CU as tillering fertilizer） on rice yield and quality. CU only was applied separately as control（CK）. Results showed that, rice grain yield, chalky kernel rate, chalky area, overall chalkiness, and the content of gliadin, glutenin, and protein, all showed the trends of UF〉PCU〉SCU within the same fertilization mode, and showed the trends of S/CRF as basal and CU as tillering fertilizer〉both S/CRF and CU as basal fertilizer within the same type of S/CRF. In contrast, the contents of amylose, amylopectin, and starch, as well as taste value, and peak and hot viscosity showed trends of SCU〉PCU〉UF, and the trends of both S/CRF and CU as basal fertilizer〉S/CRF as basal and CU as tillering fertilizer. Among S/CRF treatments and fertilization modes, taste values of cooked rice were positively correlated with amylose, amylopectin, and starch contents, as well as gel consistency, peak viscosity, hot viscosity, and cool viscosity, while negatively correlated with globulin, gliadin, glutenin, and protein contents. The types of S/CRF and fertilization modes are important for improving rice yield and quality. Compared to CK, higher yield and similar quality of rice was achieved with UF as basal and CU as tillering fertilizer, and similar yield with improved appearance and eating and cooking quality of rice was achieved with either both UF and CU as basal fertilizer, or PCU as basal and CU as tillering fertilizer.展开更多
The high-affinity K＋ （HAK） transporter gene family is the largest family in plant that functions as potassium transporter and is important for various aspects of plant life. In the present study, we identified 27 m...The high-affinity K＋ （HAK） transporter gene family is the largest family in plant that functions as potassium transporter and is important for various aspects of plant life. In the present study, we identified 27 members of this family in rice genome. The phylogenetic tree divided the land plant HAK transporter proteins into 6 distinct groups. Although the main characteristic of this family was established before the origin of seed plants, they also showed some differences between the members of non-seed and seed plants. The HAK genes in rice were found to have expanded in lineage-specific manner after the split of monocots and dicots, and both segmental duplication events and tandem duplication events contributed to the expansion of this family. Functional divergence analysis for this family provided statistical evidence for shifted evolutionary rate after gene duplication. Further analysis indicated that both point mutant with positive selection and gene conversion events contributed to the evolution of this family in rice.展开更多
It is of great significance to study the root characteristics of rice to improve water and nitrogen(N) use efficiency and reduce environmental pollution. This study investigated whether root traits and architecture of...It is of great significance to study the root characteristics of rice to improve water and nitrogen(N) use efficiency and reduce environmental pollution. This study investigated whether root traits and architecture of rice influence grain yield, as well as water and N utilization efficiency. An experiment was conducted using the upland rice cultivar Zhonghan 3(a japonica cultivar) and paddy rice cultivar Huaidao 5(also a japonica cultivar) using three N levels, namely, 2 g urea/pot(low amount, LN), 3 g urea/pot(normal amount, NN), and 4 g urea/pot(high amount, HN), and three soil water potentials(SWPs, namely, well-watered(0 kPa), mildly dried(–20 kPa) and severely dried(–40 kPa). The results showed that with decreasing SWP, the percentage of upland rice roots increased in the 0–5 cm tillage layer, and decreased in the 5–10 and 10–20 cm tillage layers, whereas paddy rice roots showed the opposite trend. With increasing amounts of N, the yield of upland and paddy rice increased, and the percentage of root volume ratios of the two rice cultivars in the 0–5 and 5–10 cm tillage layers increased, whereas that in the 10–20 cm tillage layer decreased. The roots of upland rice are mainly distributed in the 10–20 cm tillage layer, whereas most paddy rice roots are in the 0–5 cm tillage layer. These results indicate that the combination of-20 kPa SWP and NN in upland rice and 0 kPa SWP and LN in paddy rice promotes the growth of the root system during the middle and late stages, which in turn may decrease the requirements for water and N fertilizer and increase rice yield.展开更多
There is limited information about the combined effect of shading time and nitrogen （N） on grain filling and quality of rice. Therefore, two japonica super rice cultivars, Nanjing 44 and Ningjing 3, were used to stu...There is limited information about the combined effect of shading time and nitrogen （N） on grain filling and quality of rice. Therefore, two japonica super rice cultivars, Nanjing 44 and Ningjing 3, were used to study the effect of shading time and N level on the characteristics of rice panicle and grain filling as well as the corresponding yield and quality. At a low N level （150 kg N ha^-1, 150N）, grain yield decreased （by 21.07-26.07%） under the treatment of 20 days of shading before heading （BH） compared with the no shading （NS） treatment. These decreases occurred because of shortened panicle length, decreased number of primary and secondary branches, as well as the grain number and weight per panicle. At 150N, in the treatment of 20 days of shading after heading （AH）, grain yield also decreased （by 9.46-10.60%） due to the lower grain weight per panicle. The interaction of shading and N level had a significant effect on the number of primary and secondary branches. A high level of N （300 kg N ha^-1, 300N） could offset the negative effect of shading on the number of secondary branches and grain weight per panicle, and consequently increased the grain yield in both shading treatments. In superior grains, compared with 150N NS, the time to reach 99% of the grain weight （T99） was shortened by 1.6 to 1.7 days, and the grain weight was decreased by 4.18-5.91% in 150N BH. In 150N AH, the grain weight was 13.39-13.92% lower than that in 150N NS due to the slow mean and the maximum grain-filling rate （GRmean and GRmax）. In inferior grains, grain weight and GRmean had a tendency of 150N NS〉150N BH〉150N AH. Under shaded conditions, 300N decreased the grain weight due to lower GReen both in superior and inferior grains. Compared with 150N NS, the milling and appearance qualities as well as eating and cooking quality were all decreased in 150N BH and 150N AH. Shading with the high level of 300N improved the milling quality and decreased the number of chalky rice kernels, but the eating and cooking quality was reduced with increased chalky area and overall chalkiness. Therefore, in the case of short term shading, appropriate N fertilizer could be used to improve the yield and milling quality of rice, but limited application of N fertilizer is recommended to achieve good eating and cooking quality of rice.展开更多
Due to the relatively late start of organic rice(Oryza sativa L.) research in China,there is a still lack of systematic research on rice varieties,organic fertilizer management practices,and especially the mechanisms ...Due to the relatively late start of organic rice(Oryza sativa L.) research in China,there is a still lack of systematic research on rice varieties,organic fertilizer management practices,and especially the mechanisms of nitrogen(N) uptake and utilization.Three rice varieties,Nanjing 5055,Nanjing 9108,and Nanjing 46,were grown under organic farming(OF) at three organic fertilizer levels(103.2,160.8,and 218.4 kg N ha^(-1)) and conventional farming(CF) with regular chemical fertilizers.Rice grain yields,yield components,and quality,dry matter accumulation,and plant N were measured at different growth stages during the 2012 and 2013 growing seasons.Compared with CF,OF had a significantly reduced yield.Nanjing 9108 showed significant reductions in number of panicles per unit area and percentage of filled grains,and had the lowest yield.The effects of fertilizer type and application rate on dry matter accumulation during the main growth periods were significant for all varieties.The N content and uptake in organically grown rice were lower compared with those of rice under CF.The N recovery efficiency and N agronomic efficiency were significantly lower,whereas N physiological efficiency and N partial factor productivity were greater under OF than under CF.Under OF,the processing quality showed a slight but insignificant decline,protein content and gel consistency increased,and amylose content decreased compared with those under CF.Correlation analysis showed that under OF,grain yield was significantly correlated with N uptake.The medium organic fertilizer level(160.8 kg N ha^(-1)) was found to be the optimum fertilizer treatment,and Nanjing 46 appeared to be the best variety for organic rice cultivation.To increase rice grain yields and reduce the potential risk of non-point source pollution in organic agriculture,further research is needed to improve the N use efficiency in organic rice cultivation.展开更多
Grain number is crucial for analysis of yield components and assessment of effects of cultivation measures.The grain number per spike and thousand-grain weight can be measured by counting grains manually,but it is tim...Grain number is crucial for analysis of yield components and assessment of effects of cultivation measures.The grain number per spike and thousand-grain weight can be measured by counting grains manually,but it is time-consuming,tedious and error-prone.Previous image processing algorithms cannot work well with different backgrounds and different sizes.This study used deep learning methods to resolve the limitations of traditional image processing algorithms.Wheat grain image datasets were collected in the scenarios of three varieties,six background and two image acquisition devices with different heights,angles and grain numbers,1748 images in total.All images were processed through color space conversion,image flipping and rotation.The grain was manually annotated,and the datasets were divided into training set,validation set and test set.We used the TensorFlow framework to construct the Faster Region-based Convolutional Neural Network Model.Using the transfer learning method,we optimized the wheat grain detection and enumeration model.The total loss of the model was less than 0.5 and the mean average precision was 0.91.Compared with previous grain counting algorithms,the grain counting error rate of this model was less than 3%and the running time was less than 2 s.The model can be effectively applied under a variety of backgrounds,image sizes,grain sizes,shooting angles,and shooting heights,as well as different levels of grain crowding.It constitutes an effective detection and enumeration tool for wheat grain.This study provides a reference for further grain testing and enumeration applications.展开更多
A pot experiment was conducted to study the effects of different nitrogen application time （during the tillering or the booting stages） with the same nitrogen rates on the caryopsis development and grain quality of ...A pot experiment was conducted to study the effects of different nitrogen application time （during the tillering or the booting stages） with the same nitrogen rates on the caryopsis development and grain quality of rice variety Yangdao 6. The increased nitrogen fertilizer （urea）, especially applied during the booting stage, could evidently increase the milled rice rate, head rice rate and protein content in rice grains compared with the control （no nitrogen application）, and decrease chalky grain rate and amylose content. Moreover, the increased nitrogen fertilizer significantly affected the caryopsis development and enhanced the grain weight when nitrogen applied during the tillering and the booting stages, especially during the booting stage. During caryopsis development the increased nitrogen fertilizer applied during the tillering and booting stages could obviously decrease the total starch and amylose contents, but not obviously for the amylopectin content in rice grain. Increased topdressing of nitrogen fertilizer, especially applied during the booting stage, had significant effect on the development and structures of amyloplasts and proteinoplasts. That is, it could change the distribution, number and shape of amyloplasts and proteinoplasts in the endosperm cells especially in grain abdomen, Compared with the control the arrangements of amyloplasts and proteinoplasts were closer, with more numbers, higher density and less interspaces each ohter. Furthermore, most amyloplasts showed polyhedron under the increased nitrogen fertilizer level.展开更多
Side deep placement of nitrogen plays an important role in improving rice yield and nitrogen use efficiency.Few studies have examined the effects of reducing the times of nitrogen(RTN)application and reducing the nitr...Side deep placement of nitrogen plays an important role in improving rice yield and nitrogen use efficiency.Few studies have examined the effects of reducing the times of nitrogen(RTN)application and reducing the nitrogen rate(RNR)of application on rice yield and nitrogen use efficiency under side deep placement of nitrogen in paddy fields.Therefore,a field experiment of RNT and RNR treatments was conducted with nine fertilization modes during the 2018–2019 rice growing seasons in a rice–wheat cropping system of the lower reaches of the Yangtze River,China.Rice yield and nitrogen use efficiency were investigated under side deep placement of nitrogen.We found that under the same nitrogen application rate,the yield of RTN3 increased by 9.64 and 10.18%in rice varieties NJ9108 and NJ5718,respectively,compared with the farmers’fertilizer practices(FFP).The nitrogen accumulation of RTN3 was the highest at heading stage,at 11.30 t ha^(–1)across 2018 and 2019.Under the same nitrogen application rate,the N agronomic use efficiency(NAE),N physiological efficiency(NPE)and N recovery efficiency(NRE)of RTN3 were 8.1–21.28%,8.51–41.76%and 0.28–14.52%higher than those of the other fertilization modes,respectively.RNR led to decreases in SPAD value,leaf area index(LAI),dry matter accumulation,nitrogen accumulation,and nitrogen use efficiency.These results suggest that RTN3 increased rice yield and nitrogen use efficiency under the side deep placement of nitrogen,and RNR1 could achieve the goals of saving cost and increasing resource use efficiency.Two fertilization modes RTN3 and RNR1 both could achieve the dual goals of increasing grain yield and resource use efficiency and thus are worth further application and investigation.展开更多
基金grants from the National Key R&D Program of China(2016YFD0300503)the earmarked fund for China Agriculture Research System(CARS-01-27)+2 种基金the Key Research Program of Jiangsu Province,China(BE2016344)the earmarked fund for Jiangsu Agricultural Industry Technology System,China(JATS[2018]298)a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions,China
文摘Mechanical transplanting with carpet seedlings(MC) and mechanical direct seeding(MD) are newly developed planting methods, which increase in popularity and planted area each year. Knowing the difference for yield and rice quality under different planting methods is of great importance for the development of high quality and yield cultivation techniques under mechanical conditions. Therefore, three kinds of japonica rice including hybrid japonica rice, inbreed japonica rice, and soft rice were adopted as materials. And the differences in the quality of processing, appearance, cooking and eating quality, nutrition, and the rapid viscosity analyzer(RVA) profile were studied to reveal the effects of planting methods on yield and quality of different types of japonica rice. Results showed that the milled rice and head rice rates under MC was significantly higher than those under MD, and the processing quality of inbreed japonica rice was the most stable. Compared with MC, length/width ratio of rice under MD was significantly increased, and chalkiness rate, size, and degree were significantly decreased. The protein content under MD was lower than that under MC. MC showed higher peak viscosity and breakdown value than MD. The taste value was the greatest for soft rice, followed by inbreed japonica rice, and then by japonica hybrid rice, with no significant differences resulting from planting methods. Compared with MC, MD significantly improved the appearance quality, though processing quality and nutritional quality were decreased. And there was no significant difference in cooking and eating quality between MC and MD. Under different planting methods, the appearance quality of inbreed japonica rice changed the most and the processing quality was the most stable. The nutritional, cooking and eating quality of soft rice changed the least. Therefore, according to the different planting methods and market needs, selecting the appropriate rice varieties can reduce the risks in rice production and achieve good rice quality.
基金supported by the National Basic Research Program of China (2015CB150404)the National Key Research and Development Program of China (2016YFD0300206-4, 2018YFD0300801)+4 种基金the National Natural Science Foundation of China (31871559, 31671614)Young Elite Scientists Sponsorship Program by CAST (2016QNRC001)the Natural Science Foundation of the Jiangsu Higher Education Institutions (15KJA210005)the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutionsthe Top Talent Supporting Program of Yangzhou University (2015-01)
文摘In previous studies, integrative crop management (ICM) improved shoot growth and grain yield of rice (Oryza sativa L.). However, little is known about the effect of ICM on root growth and methane (CH4) emission of paddy rice. In this study, two rice varieties, Wuyunjing 24 and Yongyou 2640, were grown. A field experiment was conducted with three crop management treatments including zero nitrogen fertilization (0N), local farmer practice (LFP), and ICM. Root morphophysiological traits and CH4 emission from the paddy field were investigated. ICM significantly increased mean grain yield by 29.9%, with the effect attributed mainly to an increase in mean total number of spikelets by 26.4% compared to LFP. ICM increased root and shoot biomass, root length, number of roots, root oxidation activity (ROA), root bleeding rate, and root total and active absorbing surface area by respectively 24.4%, 25.7%, 17.1%, 9.3%, 18.7%, 29.5%, 12.1%, and 24.7%. The concentrations of malic, succinic, and acetic acids in root exudates were respectively 5.8%, 6.0%, and 10.5% higher in ICM than in LFP. Compared to LFP, ICM significantly decreased the rate of CH4 emission during emission peak stages and reduced total CH4 emission by 17.1%. The root morphophysiological traits were positively and significantly correlated with grain yield, whereas root length, specific root length, ROA, and root total and active absorbing surface area were negatively and significantly correlated with total CH4 emission. These results suggest that ICM could achieve the dual goals of increasing grain yield and reducing the greenhouse gas effect by improving the root morphology and physiological traits of paddy rice.
基金the National Natural Science Foundation of China(32071947,32071944,31871557)the Open Project of Jiangsu Key Laboratory of Crop Genetics and Physiology(YCSL202102)+1 种基金the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)the Postgraduate Research&Practice Innovation Program of Jiangsu province(KYCX21_3235).
文摘Root morphology and physiology influence aboveground growth and yield formation in rice.However,root morphological and physiological differences among rice varieties with differing nitrogen(N)sensitivities and their relationship with grain yield are still unclear.In this study,rice varieties differing in N sensitivity over many years of experiments were used.A field experiment with multiple N rates(0,90,180,270,and 360 kg ha^(-1))was conducted to elucidate the effects of N application on root morphology,root physiology,and grain yield.A pot experiment with root excision and exogenous application of 6-benzyladenine(6-BA)at heading stage was used to further verify the above effects.The findings revealed that(1)under the same N application rate,N-insensitive varieties(NIV)had relatively large root biomass(root dry weight,length,and number).Grain yield was associated with root biomass in NIV.The oxidation activity and zeatin(Z)+zeatin riboside(ZR)contents in roots obviously and positively correlated with grain yield in N-sensitive varieties(NSV),and accounted for its higher grain yield than that of NIV at lower N application rates(90 and 180 kg ha^(-1)).(2)The root dry weight required for equal grain yield of NIV was greater than that of NSV.Excision of 1/10 and 1/8 of roots at heading stage had no discernible effect on the yield of Liangyoupeijiu(NIV),and it significantly reduced yield by 11.5%and 21.3%in Tianyouhuazhan(NSV),respectively,compared to the treatment without root excision.The decrease of filled kernels and grain weight after root excision was the primary cause for the yield reduction.Root excision and exogenous 6-BA application after root excision had little influence on the root activity of NIV.The oxidation activity and Z+ZR contents in roots of NSV decreased under root excision,and the increase in the proportion of excised roots aggravated these effects.The application of exogenous 6-BA increased the root activity of NSV and increased filled kernels and grain weight,thereby reducing yield loss after root excision.Thus,the root biomass of NIV was large,and there may be a phenomenon of"root growth redundancy."Vigorous root activity was an essential feature of NSV.Selecting rice varieties with high root activity or increasing root activity by cultivation measures could lead to higher grain yield under lower N application rates.
基金We are grateful for grants from the National Key Research Program of China(2016YFD0300503)the earmarked fund for China Agriculture Research System(CARS-01-27)+3 种基金the National Natural Science Foundation of China(31971841)the Key Research Program of Jiangsu Province,China(BE2016344,BE2018355)the earmarked fund for Jiangsu Agricultural Industry Technology System,China(JATS[2018]298)the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions,China.
文摘In recent years,an increasing number of different types of japonica rice cultivars have been released in the southern rice region of China.The grain yield and quality of these new cultivars showed significant differences in large scale planting.However,the causes of the differences remain little known.Therefore,three typical types of japonica rice cultivars were used in this study to investigate their grain yield and quality.A scanning calorimeter(DSC),X-ray powder diffractometer(XRD),rapid viscosity analyzer(RVA)and taste analyzer were used to evaluate the cooking and eating properties.The results showed that the yield of non-soft hybrid japonica rice cultivars was significantly higher than that of non-soft inbred japonica rice cultivars and soft inbred japonica rice cultivars.Soft inbred japonica rice cultivars had a low amylose content and moderate protein content,which are the main reasons for the superior cooking and eating quality.In addition,the relative crystallinity of soft inbred japonica rice cultivars was significantly higher than that of non-soft inbred and non-soft hybrid japonica rice cultivars,which is considered the major factor resulting in higher transition temperature and gelatinization enthalpy(ΔH_(gel)).Non-soft hybrid japonica rice cultivars had a higher number of large starch granules than soft inbred and non-soft inbred japonica rice cultivars.The setback value(SB)and breakdown value(BD),indirectly reflecting the cooking and eating quality of the three types of japonica rice cultivars,also confirmed that soft inbred japonica rice cultivars with a low SB value and a high BD value had better palatability than the other two types.This study provides guidance for future plantation of different types of japonica rice cultivars in large rice-producing areas.
基金he National Key Research and Development Program of China(2022YFD2300304)the National Natural Science Foundation of China(31671617)a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD),China。
文摘Reducing water consumption in rice production in China without affecting grain yield and quality is a significant challenge.This study explored how various dry cultivation methods could improve rice quality while balancing yield to maintain sustainable rice production.A japonica upland rice cultivar and a japonica paddy rice cultivar were cultivated in the field with three cultivation methods:plastic film mulching dry cultivation(PFMC),bare dry cultivation(BC),and continuous flooding cultivation(CF)as control.There was no significant difference in upland rice yield between PFMC and BC,nor in paddy rice yield between PFMC and CF.Compared with CF,the two varieties'yields decreased significantly with BC.Dry cultivation,especially PFMC,could decrease the active filling period,chalky rice rate,chalkiness,amylose content,gel consistency,breakdown viscosity,the ratio of glutelin to prolamin,and leaf senescence while increasing water use efficiency,protein components content,setback viscosity,grain starch branching enzyme(Q-enzyme)activity,and average filling rate.Compared with paddy rice,upland rice had a lower yield,shorter active filling period,lower chalkiness grain rate and gel consistency,higher amylose content,breakdown viscosity,protein components content,and average filling rate.Grain Q-enzyme activity and grain-filling parameters were closely related to rice quality.Reasonable dry cultivation methods could balance yield and quality,especially by improving rice's nutritional and appearance quality.
基金supported by the Special Funds for Scientific and Technological Innovation of Jiangsu Province,China(Grant No.BE2022425)the National Natural Science Foundation of China(Grant No.72473124),the National Key Research and Development Program of China(Grant No.2022YFD1500404)+1 种基金the Jiangsu Province Postgraduate Research Innovation Program(Grant No.KYCX24-3785)a Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD),China。
文摘Currently,research on the co-application of straw charcoal and organic fertilizer made from livestock and poultry manure remains limited,despite their demonstrated benefits in enhancing soil fertility and improving plant physiological traits.To investigate the effects of straw charcoal on paddy soil,an experiment was conducted in fields with stable soil properties characterized by clay loam texture in the Middle-Lower Reaches of the Yangtze River,China,using the rice cultivar Nanjing 5055.
基金the financial support of the Jiangsu Agricultural Industry Technology System of China(JATS[2022]497)the Jiangsu Agriculture Science and Technology Innovation Fund,China(CX[23]3117)+2 种基金the Key Research&Development Program of Jiangsu Province,China(BE2021317)the National Natural Science Foundation of China(32101828)the Priority Academic Program Development of Jiangsu Higher Education Institutions,China(PAPD)。
文摘Unbalanced fertilizer application with high intensity nitrogen(N)and insufficient potassium(K)results in declining soil fertility.Balanced fertilization represents an effective approach to reduce fertilizer usage while enhancing maize yield and efficiency.This study examined two N levels(180 and 225 kg N ha^(-1),abbreviated N12 and N15)and four K treatments(0,75,150,and 75+75 kg K_(2)O ha^(-1),abbreviated K0,K5,K10,and K5+5)to investigate the effects of combined N and K application on biomass,nutrient accumulation,and remobilization characteristics in waxy maize.Results indicated that grain yield increased with higher K application at constant N levels,demonstrating an average increase of 1,254.8 kg ha^(-1)(2020)and 727.3 kg ha^(-1)(2021)compared with K0.Under identical N and K applications,K5+5 enhanced grain yield through increased kernel weight.The K5+5 treatment showed no significant difference in biomass and nutrient accumulation between N12 and N15.Compared to K10,K5+5 enhanced both the average remobilization amount(RBA)of biomass and increased RBA of N,phosphorus(P)and K.Additionally,the average remobilization efficiency(RBE)of biomass,N,P,and K in K5+5 increased by 3.3,4.6,10.6,and 4.2%,respectively.Moreover,topdressing K improved the apparent contribution to grain(AC)of biomass,N,P and K,facilitating greater nutrient transfer to grains and significantly increasing nutrient harvest index.Based on yield and fertilizer use efficiency,this study recommends optimized K application(basal and topdressing 75 kg ha^(-1))and moderate reduction in N application(from 225 to 180 kg ha^(-1))for spring-sown waxy maize production in southern China.
基金projects 2024YFD2300605 supported by National Key Research and Development Program of ChinaProjects#32572459,and#31901462 supported by National Natural Science Foundation of China+2 种基金a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions,China(PAPD)a project funded by Natural Science Foundation of the Jiangsu Higher Education Institutions of China(22KJA210005)a project funded by College Student Innovation and Entrepreneurship Training Program。
文摘Background Achieving a synergistic enhancement in both the yield and insect resistance of Bt cotton holds substantial importance for boosting farmers'income and ecological advantages.This study investigated the impact of amino acid foliar fertilizer(AAF)on the yield and Cry1Ac protein(CP)content,providing valuable insights for enhancing its productivity and insect-resistance capabilities.In 2021,Sikang 1 and Sikang 3 were treated with AAF once(A1)and water(CK)during the peak flowering stage.In 2022,AAF was sprayed one(A1),two(A2),and three(A3)times,respectively,with CK serving as the control.Results Compared with the control,the A3 treatment increased seed cotton yield(SCY)by 16.0%and CP by 40.98%at 30 days after flowering.AAF application enhanced soluble protein content(SP)and glutamate pyruvate transaminase(GPT)activity,while suppressing protease and peptidase activities.Concurrently,AAF improved sucrose metabolism through elevated sucrose content and increased activities of sucrose synthase(SS)and sucrose conversion enzyme(SCE),which were also positively correlated with yield.A lower ratio of carbon-to-nitrogen(C/N)was linked to higher yields and CP content.Path analysis confirmed that SP,GPT,SS,and SCE demonstrated positive effects on CP content and SCY,respectively.Peptidase activity had negative effects on CP and SCY.The C/N ratio had negative effects on SCY and CP,respectively.Conclusions Triple foliar application of AAF maintained lower C/N ratios with enhanced carbon metabolism and protein synthesis capacity,thereby simultaneously increasing both Cry1Ac protein content and yield in Bt cotton.These findings provide critical insights for improving both pest resistance and agronomic performance in Bt cotton cultivation.
基金funded by the National Key Research Program of China(2016YFD0300503)the Key Research Program of Jiangsu Province,China(BE2016344 and BE2018355)the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions,China。
文摘Mechanical pot-seedling transplanting is an innovatively developed transplanting method that has the potential to replace mechanical carpet-seedling transplanting. However, the initial pot-seedling transplanting machine lacked optimized density spacing and limited yield potential for japonica rice. Therefore, ascertaining the optimized density by wide-narrow rows and the appropriate transplanting method for yield formation and grain quality of japonica rice is of great importance for high-quality rice production. Field experiments were conducted using two japonica rice cultivars Nanjing 9108 and Nanjing 5055 under three transplanting methods in 2016 and 2017: mechanical pot-seedling transplanting with wide-narrow row(K, average row spacing of 30 cm);equidistant row(D, 33 cm×12 cm);and mechanical carpet-seedling transplanting(T, 30 cm×12.4 cm). In addition, five different density treatments were set in K(K1–K5, from 18.62×10~4 to 28.49×10~4 hills ha^(–1)). The results showed that the highest yield was produced by a planting density of 26.88×104 hills ha^(–1) in mechanical pot-seedling transplanting with wide-narrow row with a greater number of total spikelets that resulted from significantly more panicles per area and slightly more grain number per panicle, as compared with equidistant row, and yield among density in wide-narrow row showed a parabolic trend. Compared with mechanical carpet-seedling transplanting, the treatment of the highest yield increased yield significantly, which was mainly attributed to the larger sink size with improved filled-grain percentage and grain weight, higher harvest index, and increased total dry matter accumulation, especially the larger amount accumulated from heading stage to maturity stage. With the density in wide-narrow row decreasing, processing quality, appearance quality, and nutrition quality were all improved, whereas amylose content and the taste value were decreased. Compared with mechanical carpet-seedling transplanting, mechanical pot-seedling transplanting improved processing quality and nutrition quality, but decreased amylose content and deteriorated appearance quality. These results suggested that mechanical pot-seedling transplanting with wide-narrow row coupling produced a suitable planting density of 26.88×10~4 hills ha^(–1) and may be an alternative approach to improving grain yield and quality for japonica rice.
基金sponsored by the National Natural Science Foundation of China(31461143015,31271641,31471438)the National Key Technology Support Program of China(2014AA10A605,216YFD0300206-4)+1 种基金the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD),Chinathe Jiangsu Creation Program for Post-graduation Students,China(KYZZ15_0364)
文摘This study investigated if super rice could better cope with soil water deficit and if it could have better yield performance and water use efficiency （WUE） under alternate wetting and drying （AWD） irrigation than check rice. Two super rice cultivars and two elite check rice cultivars were grown in pots with three soil moisture levels, well watered （WW）, moderate water deficit （MWD） and severe water deficit （SWD）. Two cultivars, each for super rice and check rice, were grown in field with three irrigation regimes, alternate wetting and moderate drying （AWMD）, alternate wetting and severe drying （AWSD） and conventional irrigation （CI）. Compared with that under WW, grain yield was significantly decreased under MWD and SWD treatments, with less reduction for super rice than for check rice. Super rice had higher percentage of productive tillers, deeper root distribution, higher root oxidation activity, and greater aboveground biomass production at mid and late growth stages than check rice, especially under WMD and WSD. Compared with CI,AWMD increased, whereasAWSD decreased grain yield, with more increase or less decrease for super rice than for check rice. Both MWD and SWD treatments and eitherAWMD orAWSD regime significantly increased WUE compared with WW treatment or CI regime, with more increase for super rice than for check rice. The results suggest that super rice has a stronger ability to cope with soil water deficit and holds greater promising to increase both grain yield and WUE by adoption of moderate AWD irrigation.
基金the National Key Research Program of China(2016YFD0300503)the National Natural Science Foundation of China(31601246)+2 种基金the Major Independent Innovation Project in Jangsu Province,China(CX(15)1002)the Special Fund for Agro-scientific Research in the Public Interest,China(201303102)the Natural Science Foundation of the Jiangsu Higher Education Institutions,China(16KJB210014)
文摘Although studies on the balance between yield and quality of japonica soft super rice are limited, they are crucial for super rice cultivation. In order to investigate the effects of nitrogen application rate on grain yield and rice quality, two japonica soft super rice varieties, Nanjing 9108 （NJ 9108） and Nanjing 5055 （NJ 5055）, were used under seven N levels with the application rates of 0, 150, 187.5,225, 262.5, 300, and 337.5 kg ha^-1. With the increasing nitrogen application level, grain yield of both varieties first increased and then decreased. The highest yield was obtained at 300 kg ha^-1. The milling quality and protein content increased, while the appearance quality, amylose content, gel consistency, cooking/eating quality, and rice flour viscosity decreased. Milling was significantly negatively related with the eating/cooking quality whereas the appearance was significantly positively related with cooking/eating quality. These results suggest that nitrogen level significantly affects the yield and rice quality of japonica soft super rice. We conclude that the suitable nitrogen application rate for japonica soft super rice, NJ 9108 and NJ 5055, is 270 kg ha^-1, under which they obtain high yield as well as superior eating/cooking quality.
基金the National Key R&D Program of China(2016YFD0300503)the National Rice Industry Technology System,China(CARS-01-27)+3 种基金the National Natural Science Foundation of China(31971841)the Key Research Program of Jiangsu Province,China(BE2016344 and BE2018355)the earmarked fund for Jiangsu Agricultural Industry Technology System,China(JATS[2018]298)the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions,China。
文摘Indica-japonica hybrid rice(Oryza sativa L.) cultivars showed high yield potential and poor tasting quality when compared with common japonica rice cultivars. Large panicle is a prominent factor of high yield for indica-japonica hybrid rice cultivars, and the panicle weight varies greatly among different indica-japonica hybrid rice cultivars. It is important to research on yield and grain quality of different panicle weight indica-japonica hybrid rice cultivars. In this study, two different panicle types indica-japonica hybrid cultivars were used to research on the relation of yield and grain quality. The yields of two heavy panicle weights indica-japonica hybrid cultivars were significantly higher than that of two medium panicle weight rice cultivars. The cooking and eating quality and starch properties of different panicle type cultivars were evaluated. Yongyou 6715(medium panicle) and Yongyou 1852(heavy panicle) got the relatively higher cooking and eating quality. Rice cultivars with medium panicle weight had more large starch granules and higher relative crystallinity than cultivars with heavy panicle weight. Transition temperature and retrogradation enthalpy(ΔHret) of medium panicle type cultivars were significantly higher than that of heavy panicle type cultivars. There was no significant difference in amylose content among different panicle type cultivars. Protein content of heavy panicle type cultivar was higher than that of medium panicle type cultivar, and protein content is the main factor affect cooking and eating quality in this study. The cultivar Yongyou 6715 got the highest taste value with the lowest protein content. Thus, it is suggested that the emphasis on improving rice cooking and eating quality of indica-japonica hybrid rice cultivars is how to reduce the protein content in rice grain. According to the results of this study, medium panicle type with high grain weight is the desired panicle type for high quality indica-japonica hybrid rice breeding.
基金the National Key R&D Program of China (2016YFD0300503)the Key Research Program of Jiangsu Province, China (BE2016344)+3 种基金the National Rice Industry Technology System, China (CARS01-27)the National Nature Science Foundation of China (31701350)the Program for Scientific Elitists of Yangzhou University, Chinaa Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions, China
文摘There is limited information about the influence of slow or controlled release fertilizer（S/CRF） on rice yield and quality. In this study, japonica rice cultivar Nanjing 9108 was used to study the effects of three different S/CRFs（polymer-coated urea（PCU）, sulfur-coated urea（SCU）, and urea formaldehyde（UF）） and two fertilization modes（both S/CRF and common urea（CU） as basal fertilizer, S/CRF as basal and CU as tillering fertilizer） on rice yield and quality. CU only was applied separately as control（CK）. Results showed that, rice grain yield, chalky kernel rate, chalky area, overall chalkiness, and the content of gliadin, glutenin, and protein, all showed the trends of UF〉PCU〉SCU within the same fertilization mode, and showed the trends of S/CRF as basal and CU as tillering fertilizer〉both S/CRF and CU as basal fertilizer within the same type of S/CRF. In contrast, the contents of amylose, amylopectin, and starch, as well as taste value, and peak and hot viscosity showed trends of SCU〉PCU〉UF, and the trends of both S/CRF and CU as basal fertilizer〉S/CRF as basal and CU as tillering fertilizer. Among S/CRF treatments and fertilization modes, taste values of cooked rice were positively correlated with amylose, amylopectin, and starch contents, as well as gel consistency, peak viscosity, hot viscosity, and cool viscosity, while negatively correlated with globulin, gliadin, glutenin, and protein contents. The types of S/CRF and fertilization modes are important for improving rice yield and quality. Compared to CK, higher yield and similar quality of rice was achieved with UF as basal and CU as tillering fertilizer, and similar yield with improved appearance and eating and cooking quality of rice was achieved with either both UF and CU as basal fertilizer, or PCU as basal and CU as tillering fertilizer.
基金supported by the National Basic Research Program of China (No. 2006CB101700)the National High- tech Research and Development Program (No. 2006AA10Z165)the Program for New Century Excellent Talents in Uni-versity of China (No. NCET2005-05- 0502).
文摘The high-affinity K＋ （HAK） transporter gene family is the largest family in plant that functions as potassium transporter and is important for various aspects of plant life. In the present study, we identified 27 members of this family in rice genome. The phylogenetic tree divided the land plant HAK transporter proteins into 6 distinct groups. Although the main characteristic of this family was established before the origin of seed plants, they also showed some differences between the members of non-seed and seed plants. The HAK genes in rice were found to have expanded in lineage-specific manner after the split of monocots and dicots, and both segmental duplication events and tandem duplication events contributed to the expansion of this family. Functional divergence analysis for this family provided statistical evidence for shifted evolutionary rate after gene duplication. Further analysis indicated that both point mutant with positive selection and gene conversion events contributed to the evolution of this family in rice.
基金the National Natural Science Foundation of China(31671617)the National Key Research and Development Program of China(2016YFD0300502,2016YFD0300206 and 2018YFD0301306)the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD),China。
文摘It is of great significance to study the root characteristics of rice to improve water and nitrogen(N) use efficiency and reduce environmental pollution. This study investigated whether root traits and architecture of rice influence grain yield, as well as water and N utilization efficiency. An experiment was conducted using the upland rice cultivar Zhonghan 3(a japonica cultivar) and paddy rice cultivar Huaidao 5(also a japonica cultivar) using three N levels, namely, 2 g urea/pot(low amount, LN), 3 g urea/pot(normal amount, NN), and 4 g urea/pot(high amount, HN), and three soil water potentials(SWPs, namely, well-watered(0 kPa), mildly dried(–20 kPa) and severely dried(–40 kPa). The results showed that with decreasing SWP, the percentage of upland rice roots increased in the 0–5 cm tillage layer, and decreased in the 5–10 and 10–20 cm tillage layers, whereas paddy rice roots showed the opposite trend. With increasing amounts of N, the yield of upland and paddy rice increased, and the percentage of root volume ratios of the two rice cultivars in the 0–5 and 5–10 cm tillage layers increased, whereas that in the 10–20 cm tillage layer decreased. The roots of upland rice are mainly distributed in the 10–20 cm tillage layer, whereas most paddy rice roots are in the 0–5 cm tillage layer. These results indicate that the combination of-20 kPa SWP and NN in upland rice and 0 kPa SWP and LN in paddy rice promotes the growth of the root system during the middle and late stages, which in turn may decrease the requirements for water and N fertilizer and increase rice yield.
基金grants from the National Key Technology R&D Program of China (2016YFD0300503)the Key Research Program of Jiangsu Province, China (BE2016344)+3 种基金the earmarked fund for China Agriculture Research System (CARS-01-27)the National Nature Science Foundation of China (31701350)the Program for Scientific Elitists of Yangzhou University, Chinafunded by the Priority Academic Program Development of Jiangsu Higher Education Institutions,China
文摘There is limited information about the combined effect of shading time and nitrogen （N） on grain filling and quality of rice. Therefore, two japonica super rice cultivars, Nanjing 44 and Ningjing 3, were used to study the effect of shading time and N level on the characteristics of rice panicle and grain filling as well as the corresponding yield and quality. At a low N level （150 kg N ha^-1, 150N）, grain yield decreased （by 21.07-26.07%） under the treatment of 20 days of shading before heading （BH） compared with the no shading （NS） treatment. These decreases occurred because of shortened panicle length, decreased number of primary and secondary branches, as well as the grain number and weight per panicle. At 150N, in the treatment of 20 days of shading after heading （AH）, grain yield also decreased （by 9.46-10.60%） due to the lower grain weight per panicle. The interaction of shading and N level had a significant effect on the number of primary and secondary branches. A high level of N （300 kg N ha^-1, 300N） could offset the negative effect of shading on the number of secondary branches and grain weight per panicle, and consequently increased the grain yield in both shading treatments. In superior grains, compared with 150N NS, the time to reach 99% of the grain weight （T99） was shortened by 1.6 to 1.7 days, and the grain weight was decreased by 4.18-5.91% in 150N BH. In 150N AH, the grain weight was 13.39-13.92% lower than that in 150N NS due to the slow mean and the maximum grain-filling rate （GRmean and GRmax）. In inferior grains, grain weight and GRmean had a tendency of 150N NS〉150N BH〉150N AH. Under shaded conditions, 300N decreased the grain weight due to lower GReen both in superior and inferior grains. Compared with 150N NS, the milling and appearance qualities as well as eating and cooking quality were all decreased in 150N BH and 150N AH. Shading with the high level of 300N improved the milling quality and decreased the number of chalky rice kernels, but the eating and cooking quality was reduced with increased chalky area and overall chalkiness. Therefore, in the case of short term shading, appropriate N fertilizer could be used to improve the yield and milling quality of rice, but limited application of N fertilizer is recommended to achieve good eating and cooking quality of rice.
基金supported by the National Natural Science Foundation of China (Nos. 31201154 and 31571596)the Open Project Program of Jiangsu Province Key Laboratory of Crop Physiology,China (No. K12008)the Priority Academic Program Development of Jiangsu Higher Education Institutions,China
文摘Due to the relatively late start of organic rice(Oryza sativa L.) research in China,there is a still lack of systematic research on rice varieties,organic fertilizer management practices,and especially the mechanisms of nitrogen(N) uptake and utilization.Three rice varieties,Nanjing 5055,Nanjing 9108,and Nanjing 46,were grown under organic farming(OF) at three organic fertilizer levels(103.2,160.8,and 218.4 kg N ha^(-1)) and conventional farming(CF) with regular chemical fertilizers.Rice grain yields,yield components,and quality,dry matter accumulation,and plant N were measured at different growth stages during the 2012 and 2013 growing seasons.Compared with CF,OF had a significantly reduced yield.Nanjing 9108 showed significant reductions in number of panicles per unit area and percentage of filled grains,and had the lowest yield.The effects of fertilizer type and application rate on dry matter accumulation during the main growth periods were significant for all varieties.The N content and uptake in organically grown rice were lower compared with those of rice under CF.The N recovery efficiency and N agronomic efficiency were significantly lower,whereas N physiological efficiency and N partial factor productivity were greater under OF than under CF.Under OF,the processing quality showed a slight but insignificant decline,protein content and gel consistency increased,and amylose content decreased compared with those under CF.Correlation analysis showed that under OF,grain yield was significantly correlated with N uptake.The medium organic fertilizer level(160.8 kg N ha^(-1)) was found to be the optimum fertilizer treatment,and Nanjing 46 appeared to be the best variety for organic rice cultivation.To increase rice grain yields and reduce the potential risk of non-point source pollution in organic agriculture,further research is needed to improve the N use efficiency in organic rice cultivation.
基金mainly supported by the National Key Research and Development Program of China(2017YFD0301205)the Postgraduate Research&Practice Innovation Program of Jiangsu Province,China(KYCX18_2371)+4 种基金the National Natural Science Foundation of China(31701355 and 31671615)the China Postdoctoral Science Foundation,China(2016M600448)the Priority Academic Program Development of Jiangsu Higher Education Institutions,China(PAPD)the Yangzhou Science Foundation for Excellent Youths,China(YZ2017098)the Science and Technology Plan Projects of Yangzhou,China(YZ2016251)。
文摘Grain number is crucial for analysis of yield components and assessment of effects of cultivation measures.The grain number per spike and thousand-grain weight can be measured by counting grains manually,but it is time-consuming,tedious and error-prone.Previous image processing algorithms cannot work well with different backgrounds and different sizes.This study used deep learning methods to resolve the limitations of traditional image processing algorithms.Wheat grain image datasets were collected in the scenarios of three varieties,six background and two image acquisition devices with different heights,angles and grain numbers,1748 images in total.All images were processed through color space conversion,image flipping and rotation.The grain was manually annotated,and the datasets were divided into training set,validation set and test set.We used the TensorFlow framework to construct the Faster Region-based Convolutional Neural Network Model.Using the transfer learning method,we optimized the wheat grain detection and enumeration model.The total loss of the model was less than 0.5 and the mean average precision was 0.91.Compared with previous grain counting algorithms,the grain counting error rate of this model was less than 3%and the running time was less than 2 s.The model can be effectively applied under a variety of backgrounds,image sizes,grain sizes,shooting angles,and shooting heights,as well as different levels of grain crowding.It constitutes an effective detection and enumeration tool for wheat grain.This study provides a reference for further grain testing and enumeration applications.
基金This work was supported by the National Natural Science Foundation of China(Grant No.30070454).
文摘A pot experiment was conducted to study the effects of different nitrogen application time （during the tillering or the booting stages） with the same nitrogen rates on the caryopsis development and grain quality of rice variety Yangdao 6. The increased nitrogen fertilizer （urea）, especially applied during the booting stage, could evidently increase the milled rice rate, head rice rate and protein content in rice grains compared with the control （no nitrogen application）, and decrease chalky grain rate and amylose content. Moreover, the increased nitrogen fertilizer significantly affected the caryopsis development and enhanced the grain weight when nitrogen applied during the tillering and the booting stages, especially during the booting stage. During caryopsis development the increased nitrogen fertilizer applied during the tillering and booting stages could obviously decrease the total starch and amylose contents, but not obviously for the amylopectin content in rice grain. Increased topdressing of nitrogen fertilizer, especially applied during the booting stage, had significant effect on the development and structures of amyloplasts and proteinoplasts. That is, it could change the distribution, number and shape of amyloplasts and proteinoplasts in the endosperm cells especially in grain abdomen, Compared with the control the arrangements of amyloplasts and proteinoplasts were closer, with more numbers, higher density and less interspaces each ohter. Furthermore, most amyloplasts showed polyhedron under the increased nitrogen fertilizer level.
基金the National Key Research and Development Program of China(2018YFD0300802 and 2016YFD0200805)the Key Research Program of Jiangsu Province,China(BE2017343 and BE2018362)。
文摘Side deep placement of nitrogen plays an important role in improving rice yield and nitrogen use efficiency.Few studies have examined the effects of reducing the times of nitrogen(RTN)application and reducing the nitrogen rate(RNR)of application on rice yield and nitrogen use efficiency under side deep placement of nitrogen in paddy fields.Therefore,a field experiment of RNT and RNR treatments was conducted with nine fertilization modes during the 2018–2019 rice growing seasons in a rice–wheat cropping system of the lower reaches of the Yangtze River,China.Rice yield and nitrogen use efficiency were investigated under side deep placement of nitrogen.We found that under the same nitrogen application rate,the yield of RTN3 increased by 9.64 and 10.18%in rice varieties NJ9108 and NJ5718,respectively,compared with the farmers’fertilizer practices(FFP).The nitrogen accumulation of RTN3 was the highest at heading stage,at 11.30 t ha^(–1)across 2018 and 2019.Under the same nitrogen application rate,the N agronomic use efficiency(NAE),N physiological efficiency(NPE)and N recovery efficiency(NRE)of RTN3 were 8.1–21.28%,8.51–41.76%and 0.28–14.52%higher than those of the other fertilization modes,respectively.RNR led to decreases in SPAD value,leaf area index(LAI),dry matter accumulation,nitrogen accumulation,and nitrogen use efficiency.These results suggest that RTN3 increased rice yield and nitrogen use efficiency under the side deep placement of nitrogen,and RNR1 could achieve the goals of saving cost and increasing resource use efficiency.Two fertilization modes RTN3 and RNR1 both could achieve the dual goals of increasing grain yield and resource use efficiency and thus are worth further application and investigation.
