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EBV裂解期蛋白BZLF1和BILF1抑制Raji细胞主要组织相容性复合体的表达
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作者 郑洪玲 郭庆伟 +3 位作者 张伟 张艳 何兴波 叶冰 《基础医学与临床》 2023年第4期621-625,共5页
目的探讨EB病毒(EBV)裂解期蛋白BZLF1和BILF1是否能够抑制人B淋巴瘤细胞系Raji细胞主要组织相容性复合体(MHC)的表达。方法用佛波酯(TPA)和丁酸钠(NaB)诱导Raji细胞促使EBV激活进入裂解期,用Western bolt检测EBV裂解期的标志物BZLF1蛋... 目的探讨EB病毒(EBV)裂解期蛋白BZLF1和BILF1是否能够抑制人B淋巴瘤细胞系Raji细胞主要组织相容性复合体(MHC)的表达。方法用佛波酯(TPA)和丁酸钠(NaB)诱导Raji细胞促使EBV激活进入裂解期,用Western bolt检测EBV裂解期的标志物BZLF1蛋白的表达;RT-qPCR检测EBV进入裂解期后BZLF1和BILF1基因的表达;流式细胞测量术检测Raji细胞MHCⅡ类分子和MHCⅠ类分子的表达。电穿孔转染质粒过表达BZLF1和BILF1蛋白及siRNA技术敲低BZLF1和BILF1蛋白表达后,分别用流式细胞术及Western bolt检测Raji细胞MHCⅡ类分子和MHCⅠ类分子的表达。结果TPA/NaB能够诱导EBV激活进入裂解期,EBV进入裂解期后BZLF1和BILF1基因过表达。EBV进入裂解期以及过表达BZLF1和BILF1后,MHCⅡ类分子和MHCⅠ类分子表达均明显降低(P<0.05);特异性siRNA可以阻断BZLF1和BILF1对Raji细胞MHCⅡ类分子和MHCⅠ类分子的抑制作用(P<0.05)。结论EBV裂解期蛋白BZLF1和BILF1能够抑制Raji细胞MHC分子的表达。 展开更多
关键词 BZLF1 BILF1 主要组织相容性复合体Ⅱ 主要组织相容性复合体Ⅰ
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Analysis of Water Quality Variation and Trend in the Ji'an Section of the Ganjiang River
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作者 Yingjie XIAO Fengxiang LANG +3 位作者 Peng XU Xiaoyan ZOU Wu ZOU Yan MAO 《Meteorological and Environmental Research》 CAS 2021年第6期59-67,共9页
Based on the monitoring data of water quality in the Ji'an section of the Ganjiang River from 2008 to 2018 by the Water Resources Monitoring Center of Ji'an City,single factor evaluation method,comprehensive p... Based on the monitoring data of water quality in the Ji'an section of the Ganjiang River from 2008 to 2018 by the Water Resources Monitoring Center of Ji'an City,single factor evaluation method,comprehensive pollution index method and seasonal Kendall test method were adopted to study and analyze the characteristics of surface water resources and the changing trend of water quality in the river section.The results show that the water quality in the Ji'an section of the Ganjiang River was generally good,and the water quality of each function zone reached the target requirement.The comprehensive pollution index was controlled between 0.21 and 0.40 over the years,and there was no significant change in time and space,and the water quality was relatively stable.The dissolved oxygen and permanganate index tended to improve,and there was no change in five-day biochemical oxygen demand,while ammonia nitrogen and total phosphorus showed an upward trend.The water quality as a whole showed a trend of deterioration.By discussing the influencing factors of the characteristic pollutants,the causes of water quality in the Ji'an section of the Ganjiang River were further analyzed.It is suggested to strengthen the supervision and control of non-point source and point source pollution. 展开更多
关键词 Ji'an section of the Ganjiang River Water function zone Ammonia nitrogen Total phosphorus Changing trend
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Differentiation and restructuring-new space for development of urban center area:a case study of Shizhong District of Ji'nan City
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作者 Shi Xiaoyan Wu Meichuang 《Ecological Economy》 2009年第3期248-258,共11页
Taking the development tendency of current world industrial development as the background, this paper restructures the internal space of urban center area by illustrating reasons for internal space differentiation of ... Taking the development tendency of current world industrial development as the background, this paper restructures the internal space of urban center area by illustrating reasons for internal space differentiation of urban center area, that is to say, according to the tertiary industry interior's high-end of value chain after convergence among the primary, the secondary and the tertiary industries, the space structure of urban center area will form the space development mode with characteristic modern service industry cluster districts through vertical segmentation, horizontal derivatization and block aggregation and realize perfect space renewal of urban center area in the end. 展开更多
关键词 Urban center area DIFFERENTIATION RESTRUCTURING Shizhong District Ji'nan
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The Application of Traditional Chinese Medicine Food Therapy Combined with Baduanjin in the Nursing of Constipation Patients
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作者 XU Ying SU Xiaojie BAO Jinlei 《外文科技期刊数据库(文摘版)医药卫生》 2021年第11期430-432,共5页
Objective: to explore the effect of TCM food therapy combined with Baduanjin in nursing constipation patients. Methods: 82 patients with functional constipation who entered a hospital in Qingyang City from October 202... Objective: to explore the effect of TCM food therapy combined with Baduanjin in nursing constipation patients. Methods: 82 patients with functional constipation who entered a hospital in Qingyang City from October 2020 to February 2021 were selected by convenient sampling for nursing intervention. The experimental group was given Chinese medicine food therapy combined with Baduanjin, and the control group was given general nursing. The intervention lasted 14 days. The scores of symptom checklist 90 (SCL-90) and quality of life analysis of constipation patients were recorded. Results: compared with the control group, the experimental group can significantly reduce the scores of symptom checklist 90 and quality of life analysis of constipation patients, indicating that the experimental group is better than the control group in improving the symptoms of constipation patients (P < 0.05). Conclusion: the experimental group can significantly reduce the scores of SCL-90 and QOL analysis of constipation patients, and the intervention results are better than the control group. 展开更多
关键词 traditional Chinese medicine food therapy BADUANJIN CONSTIPATION
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Comparison Research of Neoproterozoic Strata in Liaodong and Jiaobei Regions
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作者 LIULiping XUWentian +1 位作者 LAIHongyu WANGChunguang 《外文科技期刊数据库(文摘版)工程技术》 2020年第2期116-119,共6页
The research object of this paper is Neoproterozoic strata in Liaodong and Jiaobei. Through collecting data, sorting out data, making maps, analyzing maps, comparative research, summarizing and so on, the comparative ... The research object of this paper is Neoproterozoic strata in Liaodong and Jiaobei. Through collecting data, sorting out data, making maps, analyzing maps, comparative research, summarizing and so on, the comparative study of Neoproterozoic strata characteristics in the two areas is completed. Based on the knowledge of petrology and stratigraphy, as well as the stratigraphic histogram drawn by the collected stratigraphic lithology of the two areas, through the comparative analysis of the lithologic characteristics of the two areas, it is concluded that the Baoshankou formation, Fuziguang formation, Nanzhuang formation and Xiangkuang formation in Jiaobei correspond to Nanfen formation, Qiaotou formation, Changlingzi formation and Nanguanling formation in Liaodong. 展开更多
关键词 NEOPROTEROZOIC stratigraphic division and correlation Liaodong and Jiaobei
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Effect of Fujian tablet on the expression of Nogo-A mRNA in the cervical spinal cord of middle cerebral artery occlusion model rats 被引量:8
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作者 Wei Liu Guo li Zhang 《Neural Regeneration Research》 SCIE CAS CSCD 2007年第10期599-602,共4页
Inhibiting the expression of Nogo-A in cervical spinal cord by use of interaction of antigen and antibody can help the remodeling of corticospinal projection of focal cerebral ischemia model rats to facilitate neurolo... Inhibiting the expression of Nogo-A in cervical spinal cord by use of interaction of antigen and antibody can help the remodeling of corticospinal projection of focal cerebral ischemia model rats to facilitate neurological recovery, which provides a new possible mechanism for drugs to promote neurological recovery. However, the effects of drugs on the expression of Nogo-A in cervical spinal cord are still unclear. OBJECTIVE: To observe the effect of Fujian tablet on the expression of Nogo-A mRNA in cervical spinal cords of middle cerebral artery occlusion (MCAO) rats, and to investigate the possible regulatory effect of Fujian tablet on the regenerated microenvironment of spinal conduction bundle. DESIGN: A randomized and controlled trial taking Wistar rats as experimental animals. SETTING: Department of Neurology, Affiliated Hospital of Shandong University of Traditional Chinese Medicine. MATERIALS: This experiment was carried out in the laboratory of Shandong Academy of Medical Science between June 2005 and July 2006. A total of 40 healthy male Wistar rats, aged 12 weeks, weighing 250 - 300 g, were provided by the Experimental Animal Center of Shandong University. Fujian tablets (main components: Heshouwu, Yinyanghuo, etc) were provided by office of Pharmaceutics of Shandong University of traditional Chinese medicine. Nogo-A detection kit was provided by Wuhan Boster Biotechnology Co.,Ltd., and batch number was 040309009. This experiment was approved by Local Animal Ethics Committee. METHODS: Forty male rats were randomly divided into 4 groups, with 10 in each: normal group, sham-operation group, model group and administration group. Rats in the administration group and model group were subjected to MCAO. Rats in the sham-operation group underwent the same craniotomy, and their middle cerebral arteries (MCA) were not occluded. Rats in the normal group were untouched. Rats in administration group were intragastrically administrated with the solution of Fujian tablet at a dose of 9 g/kg and others were given equal dosage of normal saline two days later. The treatments were done once a day and the course totaled 2 weeks. MAIN OUTCOME MEASURES: The expression of Nogo-A mRNA in slices of cervical spinal cords. RESULTS: Forty rats were involved in the final analysis. The expression of Nogo-A mRNA in the cervical spinal cord of rats in the administration group and model group was significantly decreased as compared with that in the normal group (P 〈 0.01 and P 〈 0.05, respectively). The expression of Nogo-A mRNA in the administration group was also significantly weaker than that in the model group (P 〈 0.05 ) . CONCLUSION: Fujian tablet can inhibit the expression of Nogo-A mRNA in cervical spinal cords of MCAO rats, which facilitates regeneration and remodeling of cervical spinal cords. 展开更多
关键词 middle cerebral artery occlusion spinal cord animal experiment
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He–Ar Isotopic Tracing of Pyrite from Ore-forming Fluids of the Sanshandao Au Deposit, Jiaodong Area 被引量:7
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作者 HAN Zhenyu YU Xiaowei +4 位作者 LI Shoujun Tian Jingxiang WANG Zhongliang YU Xiaojing WANG Ligong 《Acta Geologica Sinica(English Edition)》 SCIE CAS CSCD 2019年第6期1797-1807,共11页
The Sanshandao Au deposit is located in the famous Sanshandao metallogenic belt,Jiaodong area.To date,accumulative Au resources of 1000 t have been identified from the belt.Sanshandao is a world-class gold deposit wit... The Sanshandao Au deposit is located in the famous Sanshandao metallogenic belt,Jiaodong area.To date,accumulative Au resources of 1000 t have been identified from the belt.Sanshandao is a world-class gold deposit with Au mineralization hosted in Early Cretaceous Guojialing-type granites.Thus,studies on the genesis and ore-forming element sources of the Sanshandao Au deposit are crucial.He and Ar isotopic analyses of fluid inclusions from pyrite(the carrier of Au)indicate that the fluid inclusions have 3 He/4 He=0.043–0.21 Ra with an average of 0.096 Ra and 40 Ar/36 Ar=488–664 with an average of 570.8.These values represent the initial He and Ar isotopic compositions of ore-forming fluids for trapped fluid inclusions.The comparison of H–O isotopic characteristics combined with deposit geology and wall rock alteration reveals that the ore-forming fluids of the Sanshandao Au deposit show mixed crust–mantle origin characteristics,and they mainly comprise crust-derived fluid mixed with minor mantle-derived fluid and meteoric water during the uprising process.The ore-forming elements were generally sourced from pre-Cambrian meta-basement rocks formed by Mesozoic reactivation and mixed with minor shallow crustal and mantle components. 展开更多
关键词 Sanshandao Au deposit He and Ar isotopes fluid inclusions crustal fluid mantle-derived fluid
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Research on the Variation of Population Distribution and Its Characteristics Based on Spatial Autocorrelation Method: A Case Study of Poyang Lake Region in Jiangxi Province
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作者 Luo Hui Yang Weichun 《Chinese Journal of Population,Resources and Environment》 2010年第4期76-78,共3页
According to Statistical Yearbook of Jiangxi Province(2001~2006),We analyze the time-space variation of population distribution of Poyang Lake region from the two points of view.The former is quality of population,wh... According to Statistical Yearbook of Jiangxi Province(2001~2006),We analyze the time-space variation of population distribution of Poyang Lake region from the two points of view.The former is quality of population,which involves culture structure,occupational structure,age structure and sex structure of population.The latter is quantity of population,which only involves the amount of population.Furthermore,we can reveal the internal relations and action mechanism of variation of population distribution by analyzing the regional economic development,population urbanization,land use and ecological landscape of Poyang Lake region.It is important to provide help for region planning,ecological landscape planning and environmental protection by correct understanding the man-land relationship of natural-human ecosystem in Poyang Lake region. 展开更多
关键词 population distribution spatial autocorrelation changing characteristics Poyang Lake region
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Spatial and Temporal Variation and Trend Analysis of Water Quality in Large Reservoirs in Ji’an City
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作者 MAO Yan ZHANG Jie +4 位作者 BAN Lei LANG Fengxiang MIN Xiang NIU Ziqiang ZHOU Yin 《Journal of Landscape Research》 2023年第5期48-52,共5页
Based on the water quality monitoring data of 5 large reservoirs in Ji’an City,Jiangxi Province from 2015-2021,the temporal and spatial variation characteristics and trends of water quality of the reservoirs were ana... Based on the water quality monitoring data of 5 large reservoirs in Ji’an City,Jiangxi Province from 2015-2021,the temporal and spatial variation characteristics and trends of water quality of the reservoirs were analyzed by single-factor evaluation method and seasonal Kendall test to evaluate the trophic status of the reservoirs and explore the influencing factors of characteristic pollutants.The results showed that:①the water quality of the reservoirs was good and could meet the water needs of various functions;②the water quality of the reservoirs had generally changed from bad to good in recent years,indicating that the implementation of“river chief system”has achieved certain results;③Kendall test analysis showed that,except for individual projects which showed an upward trend in water quality,other projects showed no obvious change trend or downward trend,indicating that the water quality of the reservoirs is indeed improving;④the causes of water pollution in reservoir area were further analyzed by exploring the natural and human factors of the characteristic pollutant total phosphorus.It is recommended to strengthen supervision in the later stage to control point and non-point source pollution. 展开更多
关键词 Water quality evaluation Single factor evaluation Seasonal Kendall test Large reservoir Trophic status Water quality trends
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Analysis of Unpaid Blood Donors with Positive Anti-HIV Confirmation Results in Ji’nan
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作者 HANPixu LIXuejing 《外文科技期刊数据库(文摘版)医药卫生》 2022年第3期034-038,共5页
Objective: to eliminate the risk of blood donation in high-risk groups. In order to reduce this series of risks, Ji'nan Blood Supply and Insurance Center conducted laboratory tests for anti-HIV primary screening f... Objective: to eliminate the risk of blood donation in high-risk groups. In order to reduce this series of risks, Ji'nan Blood Supply and Insurance Center conducted laboratory tests for anti-HIV primary screening for unpaid blood donors from January 2015 to September 2021.Methods: a total of 259 blood samples were collected and sent to the laboratory of Ji'nan Center for Disease Control and Prevention for confirmation for 100% anti-HIV testing. In addition, the distribution of their blood donation times, age, gender, occupation, education, transmission route and native origin were summarized and analyzed. Results: 40 positive, 26 to 40 (47.5%), male blood donors (97.5%), college degree or above (55%), same-sex group (77.5%) were the majority;Most infections were initial blood donation (92.5%);HIV infection was relatively high in all occupations (10%).Conclusion: the blood safety and quality is especially important to establish a fixed and volunteer team of low-risk unpaid blood donors. Medical staff should strengthen the HIV screening of blood donors, improve the blood safety and quality, in order to do a good job of AIDS, high-risk behavior, confidential blood abandonment, and safe blood donation related knowledge publicity, do a good job of communication with blood donors and the evaluation of blood donors. 展开更多
关键词 unpaid blood donation ANTI-HIV low risk population safe blood donation
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A Survey on the Present Situation of Interpersonal Communication among Problem Pupils in Schools ——A Case Study of Ji'nan Area A
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作者 MAO Mingyue ZHANG Wenjing 《外文科技期刊数据库(文摘版)教育科学》 2021年第10期029-031,共5页
Interpersonal communication in schools is the main form of communication and interaction between students and people, and is also an important part of school education. Good interpersonal communication in schools can ... Interpersonal communication in schools is the main form of communication and interaction between students and people, and is also an important part of school education. Good interpersonal communication in schools can effectively promote the social development of students and lay a foundation for the establishment of good interpersonal relationship in the future. On-campus interpersonal communication of problem pupils is of more important significance to their behavioral, emotional and psychological development, but it is easy to be ignored. Therefore, we need to actively pay attention to on-campus interpersonal communication of problem pupils in order to promote the effective transformation of problem pupils. 展开更多
关键词 problem pupils interpersonal communication in school status quo of communication
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Typical Survey and Research on Returning-home Migrant Workers'Entrepreneurship in Jiangxi Province under Post-financial Crisis
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作者 OUYANG Zhang-sun ZHANG Xin-zhi +3 位作者 WANG Run-qun LUO Feng SHEN Ai-guo WU Xing-lin 《Asian Agricultural Research》 2010年第10期34-37,共4页
In order to study the entrepreneurship situation of Jiangxi return migrant workers after the financial crisis,we have conducted a survey on 107 entrepreneurial return migrant workers in 6 counties of Jiangxi Province.... In order to study the entrepreneurship situation of Jiangxi return migrant workers after the financial crisis,we have conducted a survey on 107 entrepreneurial return migrant workers in 6 counties of Jiangxi Province.The results show that the return of migrant workers is mainly attributed to personal factors,while social factors are also very important.The major difficulties facing return migrant workers are the funding difficulties,complicated formalities to start a business,difficulty in safeguarding their rights and interests,their own low quality and so on.The support needed by the return migrant workers from the government is manifested in five aspects,say,to improve the service and management for the entrepreneurship of migrant workers,to provide financing policy support,to increase the preferential fiscal and taxation policies,to enhance the entrepreneurship training and to provide preference for the return migrant workers to use land. 展开更多
关键词 Post-financial crisis Returning-home migrant workers'entrepreneurship Typical survey Entrepreneurial motivation Jiangxi Province China
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Pathogenic Bacteria Detection in Parasitic Fleas—Jiangxi Province,China,2023
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作者 Lijuan He Zhiyun Liu +4 位作者 Like Duan Lin Zhang Xuexia Hou Jingwen Li Qin Hao 《China CDC weekly》 2025年第16期537-543,共7页
Objective:Fleas are vectors for the transmission of various pathogens.The study is to understand the pathogens carried by parasitic fleas in domestic dogs and to evaluate the pathogenic potential risk to humans.Method... Objective:Fleas are vectors for the transmission of various pathogens.The study is to understand the pathogens carried by parasitic fleas in domestic dogs and to evaluate the pathogenic potential risk to humans.Methods:121 fleas were collected from 6 dogs in different farmers’households in Suichuan County,Ji’an City,Jiangxi Province in July 2023.Flea species were determined through morphological identification and CoII gene detection.Whole genomic DNA was extracted from all 121 fleas,and six pathogens-Borrelia burgdorferi,Borrelia miyamotoi,Anaplasma phagocytophilum,Coxiella burnetii,spotted fever group Rickettsia,and Ehrlichia chaffiensis—were detected using nested polymerase chain reaction(PCR).Results:Positive products were sequenced,and the carrier status of each pathogen was analyzed.Of the 121 fleas identified,118 were Pulex irritans and 3 were Ctenocephalides felis.PCR results revealed that Borrelia burgdorferi(5%,6/118),Borrelia miyamotoi(0.8%,1/118),Borrelia hermsii(9%,11/118),Anaplasma phagocytophilum(0.8%,1/118),spotted fever group Rickettsia(0.8%,1/118),and Coxiella burnetii(0.8%,1/118)were detected in Pulex irritans.Additionally,one sample showed mixed infection with both Borrelia burgdorferi and Anaplasma phagocytophilum(0.8%,1/118).Conclusions:This study suggests that Pulex irritans can carry multiple pathogens,with implications for public health needs that warrant further investigation. 展开更多
关键词 pathogenic bacteria parasitic fleas public health morphological identification domestic dogs coii gene detectionwhole genomic dna FLEAS Jiangxi province
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防寒靴热湿舒适性能的主客观综合评测试点研究
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作者 弓太生 李文博 +3 位作者 杜美娴 吴婷 周素静 刘世超 《皮革科学与工程》 北大核心 2026年第1期80-87,共8页
【目的】为探究防寒靴整鞋热湿舒适性及鞋帮材料、环境温度对防寒靴热湿舒适性的影响。【方法】使用气候仓模拟0、-5和-10°C三个温度条件,用传感器测量两款防寒靴鞋腔的温度和相对湿度,同时分别采用四点和七点强度等级量表进行闷... 【目的】为探究防寒靴整鞋热湿舒适性及鞋帮材料、环境温度对防寒靴热湿舒适性的影响。【方法】使用气候仓模拟0、-5和-10°C三个温度条件,用传感器测量两款防寒靴鞋腔的温度和相对湿度,同时分别采用四点和七点强度等级量表进行闷湿感和热暖感评价。【结果】在三种环境温度下,气凝胶防寒靴各部位的温度都高于17防寒靴2~4°C。气凝胶防寒靴的相对湿度略高于17防寒靴,17防寒靴的平均吸湿量高于气凝胶防寒靴1.39 g。气凝胶防寒靴的热湿舒适性主观评价优于17防寒靴。【结论】气凝胶保温棉可提高鞋靴的保温性能、减轻鞋靴质量。低温环境下Coolmax做内里材料仍有较好的吸湿排汗能力,可提高防寒靴的湿舒适性能。在防寒靴的热湿舒适性测评中,受试者对潮湿和足部不适的感知与高排汗部位并不对应。 展开更多
关键词 防寒靴 热湿舒适性 鞋腔内温湿度 气候仓 主客观评价 革制品
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考虑出砂影响的榆37储气库合理注采参数设计
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作者 王萍 卫永晟 +5 位作者 郭燕妮 汤诗棋 黄海 屈展 王亮 何雅文 《新疆石油地质》 北大核心 2026年第1期64-73,共10页
储气库注采井反复高强度注采,储集层频繁应力变化,易引发出砂,威胁储气库稳定运行。以鄂尔多斯盆地榆37储气库为例,运用节点分析法,考虑支撑剂运移的临界流速模型,结合地层出砂预测的临界出砂压差模型及临界冲蚀流量模型,明确榆37储气... 储气库注采井反复高强度注采,储集层频繁应力变化,易引发出砂,威胁储气库稳定运行。以鄂尔多斯盆地榆37储气库为例,运用节点分析法,考虑支撑剂运移的临界流速模型,结合地层出砂预测的临界出砂压差模型及临界冲蚀流量模型,明确榆37储气库注采井的合理注采区间,确保储气库在极限生产状态下的运行安全。结果表明:通过对储集层裂缝中支撑剂进行受力分析,建立裂缝中支撑剂启动的临界流速模型,计算出当注入速率与采出速率分别为10.89 m/s和8.19 m/s时,支撑剂达到临界启动流速;考虑3种限制性模型,对节点分析法进行改进,计算出榆43-1井安全运行的合理注采区间,分别为1.79×10~4~6.53×10^(4) m^(3)/d和2.82×10~4~6.35×10^(4) m^(3)/d;在安全的前提下,计算出榆37储气库10口井的合理注采区间。 展开更多
关键词 榆37储气库 储气库出砂 节点分析法 支撑剂启动 临界流速 临界出砂压差 冲蚀流量
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促进组织工程血管移植物早期阶段形成的无血清培养基
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作者 陈颖 孙盱衡 +3 位作者 刘青 肖聪 蒋虹婧 林展翼 《中国组织工程研究》 北大核心 2026年第20期5093-5102,共10页
背景:在组织工程领域,利用平滑肌细胞进行三维培养来构建血管移植物具有重要意义。然而,当前广泛采用的含胎牛血清培养基存在道德、安全以及成分复杂性等潜在问题,可能影响实验结果,不利于血管移植物的临床应用。目的:选定一种成分明确... 背景:在组织工程领域,利用平滑肌细胞进行三维培养来构建血管移植物具有重要意义。然而,当前广泛采用的含胎牛血清培养基存在道德、安全以及成分复杂性等潜在问题,可能影响实验结果,不利于血管移植物的临床应用。目的:选定一种成分明确的无血清培养基替代传统含血清培养基用于平滑肌细胞培养,评估无血清培养基用于构建组织工程血管的可行性。方法:采用组织块培养法分离培养牛胸主动脉平滑肌细胞,取第3-8代细胞用于后续实验。配制无血清培养基:含5 mg/mL牛血清白蛋白、50μg/mL L-抗坏血酸、0.4μg/mL胎球蛋白、15 ng/mL碱性成纤维细胞生长因子、15 ng/mL血小板源性生长因子BB和10μg/mL胰岛素-5.5μg/mL转铁蛋白-67ng/mL亚硒酸钠的DMEMF12培养基。①二维培养:将血管平滑肌细胞分4组培养,分别加入无血清培养基与含体积分数0%,5%,10%胎牛血清的DMEM F12培养基,观察细胞形态,通过CCK-8、EdU、CytoCube细胞计数仪、流式细胞仪检测细胞增殖情况,羟脯氨酸定量法检测总胶原蛋白含量,免疫荧光染色与qRT-PCR检测平滑肌细胞收缩表型标志物钙调蛋白1、α-平滑肌肌动蛋白表达,免疫印迹检测Ⅰ型胶原、Ⅲ型胶原表达,划痕实验检测细胞迁移。②三维培养:将血管平滑肌细胞接种于聚乙醇酸支架上分4组培养,分别加入无血清培养基与含体积分数0%,5%,10%胎牛血清的DMEM F12培养基,扫描电镜分析组织外部形态,苏木精-伊红与Masson染色观察组织形态,免疫荧光染色检测组织Ⅰ型/Ⅲ型胶原表达,羟脯氨酸定量法检测总胶原蛋白含量。结果与结论:①二维培养:倒置显微镜下可见0%血清培养组细胞密度最低、细胞体积较大,对比5%,10%血清培养组,无血清培养组细胞呈现更细长的梭形,细胞排列更规则。CCK-8、EdU、CytoCube细胞计数仪、流式细胞仪检测显示,无血清培养组细胞增殖少于10%血清培养组,多于0%,5%血清培养组。5%,10%血清培养组与无血清培养组钙调蛋白1、α-平滑肌肌动蛋白表达均低于0%血清培养组,细胞迁移能力强于0%血清培养组。无血清培养组总胶原蛋白含量高于其他3组。10%血清培养组、无血清培养组Ⅲ型胶原表达高于0%,5%血清培养组。②三维培养:扫描电镜下可见各组平滑肌细胞聚乙醇酸支架上形成了组织工程血管支架的早期组织,其中无血清培养基组组织中细胞外基质沉积最多。无血清培养基组组织中总胶原蛋白含量与Ⅰ型、Ⅲ型胶原表达显著高于其他3组。苏木精-伊红与Masson染色显示,除0%血清培养组外,其余3组均可见组织工程血管支架的形成。③结果表明,无血清培养基可促进体外血管平滑肌细胞的增殖、促进组织工程血管移植物的形成。 展开更多
关键词 组织工程血管移植物 血管平滑肌细胞 无血清培养基 胶原蛋白 增殖 胎牛血清 生物材料
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高动水卵石地层换乘厅接续建设降止水技术研究
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作者 陈贤波 寇鼎涛 +1 位作者 吴圣智 牛帅卿 《价值工程》 2026年第1期101-103,共3页
在高动水卵石地层此类复杂地下环境中开展地铁、隧道等地下工程施工时,经常面临突涌水事故的发生,突涌水严重威胁工程施工质量,会对地下工程造成严重损坏,依托北京地铁19号线一期工程右安门外站换乘厅工程,针对地铁基坑降水工况,开展了... 在高动水卵石地层此类复杂地下环境中开展地铁、隧道等地下工程施工时,经常面临突涌水事故的发生,突涌水严重威胁工程施工质量,会对地下工程造成严重损坏,依托北京地铁19号线一期工程右安门外站换乘厅工程,针对地铁基坑降水工况,开展了高水位卵石地层降水和注浆止水帷幕施工技术研究,对降水和注浆止水帷幕方案进行了整体效果验证和总结。研究表明:生态补水导致城市地下水位持续上升使地下工程在建过程中面临被地下水侵入的风险,常规降水方案无法满足降水要求;基坑内狭小空间下降止水结合的注浆施工技术能有效降低地下水位;水性聚氨酯抗动水冲刷性能强,在注浆后整体留存率高且可形成良好的凝胶体,可与耐久性好的封底硫铝酸盐水泥共同构建起整体围蔽式止水帷幕。 展开更多
关键词 基坑 降水条件 高水位卵石地层 帷幕注浆止水 控制要点 效果分析
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An Outbreak of Dengue Virus Type 1--Jiangxi Province,China,2019 被引量:1
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作者 Fuqiang Yang Guanghui Xia +12 位作者 Zhili Zeng Zhongjian Li Huijian Cheng Daping Che Jun Zong Jianxiong Li Tian Gong Guihua Liu Weidong Zhou Bihai Wei Xiaobo Liu Jiandong Li Sheng Ding 《China CDC weekly》 2020年第35期684-687,共4页
What is known about this topic?Dengue fever is an acute febrile illness caused by four types of dengue virus(DENV 1–4),and is a mosquitoborne infectious disease.The incidence of dengue has increased dramatically arou... What is known about this topic?Dengue fever is an acute febrile illness caused by four types of dengue virus(DENV 1–4),and is a mosquitoborne infectious disease.The incidence of dengue has increased dramatically around the world in recent decades.An estimated of 3.9 billion people in 128 countries are at risk of infection with dengue viruses,70%of whom are in Asia.In 2017,the first local infections of dengue virus(DENV-2)in Jiangxi Province was reported in Zhanggong District,Ganzhou City.What is added by this report?From August to September 2019,the first dengue outbreak happened in Xin’gan County,where 81 local dengue fever cases were reported,35 were laboratoryconfirmed cases,and 46 were clinically-diagnosed cases;all cases were dengue virus type 1.The DENV-virus strains isolated from the cases of Xin’gan County were close to the strains isolated in Singapore(MF033254|25657|Singapore|2016)and Henan(MK905537|He nan201903|China:Henan Province|2019).What are the implications for public health practice?The outbreak might have been caused by imported cases or covert infections.The outbreak in Xin’gan County indicated that more proactive countermeasures should be taken during the dengue epidemic period such as intensifying surveillance for dengue cases,virus serotype,and Aedes vector density and strengthening cooperation with customs and tourism departments.Moreover,the outbreak should prompt training in medical institutions and improved ability for doctor’s to diagnose dengue fever,which is very important for early detection of dengue cases and taking preventive measures. 展开更多
关键词 PROMPT strengthening JIANGXI
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锂矿石冶炼渣关键金属提取与高值化研究进展
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作者 王振州 游延峰 +7 位作者 丁超阁 何承瑞 姚丽 刘添 魏冬冬 陈素华 杨利明 罗旭彪 《能源环境保护》 2026年第1期68-79,共12页
锂矿冶炼渣是当前火法-湿法提锂主流工艺中产生的主要大宗固体副产物。围绕其高效资源化与高值化利用的技术研究,对降低我国锂资源对外依存度、保障新能源产业链安全稳定运行、推动绿色低碳发展至关重要。目前,相关研究已采用火法-湿法... 锂矿冶炼渣是当前火法-湿法提锂主流工艺中产生的主要大宗固体副产物。围绕其高效资源化与高值化利用的技术研究,对降低我国锂资源对外依存度、保障新能源产业链安全稳定运行、推动绿色低碳发展至关重要。目前,相关研究已采用火法-湿法联用、碱焙烧等多种技术,以增强锂的浸出动力学,并实现铷、铯、铝等伴生战略金属的协同回收。同时,锂矿冶炼渣在制备高性能三元地聚合物、水泥及分子筛等高附加值材料方面也展现出潜力,但仍存在锂回收率不高、战略金属回收率低、产品附加值有限及相关标准体系不健全等关键瓶颈问题。为此,系统分析了锂冶炼渣的化学组成、赋存形态等关键物化特性,综述了锂、铷、铯等有价金属的浸出、选择性吸附及电化学回收等主流回收技术,并总结了其在制备三元地聚合物、水泥等高附加值产品的研究进展与存在的问题。进一步提出以源头减量–过程低碳–末端高值为核心的可持续技术路径,着重从快速活化、多组分选择性分离及全流程系统集成等技术与机理方面,为锂矿冶炼渣的绿色循环利用和高值化技术开发提供借鉴。 展开更多
关键词 锂矿冶炼渣 锂提取 资源化 高值化 胶凝材料
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血清PDK1、PAX8、DKK1对子宫内膜异位症的诊断价值
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作者 刘敏 沈承承 《检验医学与临床》 2026年第1期38-42,共5页
目的探讨子宫内膜异位症(EMs)患者血清3-磷脂酰肌醇依赖性蛋白激酶1(PDK1)、配对盒基因8(PAX8)、dickkopf相关蛋白1(DKK1)水平及其诊断价值。方法选取2021年12月至2024年12月该院收治的100例EMs患者作为观察组,根据病情严重程度分为轻度... 目的探讨子宫内膜异位症(EMs)患者血清3-磷脂酰肌醇依赖性蛋白激酶1(PDK1)、配对盒基因8(PAX8)、dickkopf相关蛋白1(DKK1)水平及其诊断价值。方法选取2021年12月至2024年12月该院收治的100例EMs患者作为观察组,根据病情严重程度分为轻度组(43例)和中重度组(57例);另选取同期在该院体检的100例女性健康体检者作为对照组。采用酶联免疫吸附试验检测所有研究对象血清PDK1、PAX8、DKK1水平。采用Pearson相关分析EMs患者血清PDK1、PAX8、DKK1水平与美国生育医学会修正子宫内膜异位症分期(r-AFS)评分的相关性。采用多因素Logistic回归分析EMs发生的影响因素。采用受试者工作特征(ROC)曲线分析血清PDK1、PAX8、DKK1对EMs的诊断价值。结果观察组痛经患者占比,以及血清PDK1、PAX8、DKK1水平均高于对照组,差异均有统计学意义(P<0.05)。中重度组血清PDK1、PAX8、DKK1水平及r-AFS评分均高于轻度组,差异均有统计学意义(P<0.05)。Pearson相关分析结果显示,EMs患者血清PDK1、PAX8、DKK1水平与r-AFS评分均呈正相关(r=0.411、0.557、0.531,P<0.05)。多因素Logistic回归分析结果显示,血清PDK1、PAX8、DKK1水平升高均为EMs发生的危险因素(P<0.05)。ROC曲线分析结果显示,血清PDK1、PAX8、DKK1联合诊断EMs的曲线下面积(AUC)为0.893,大于3项单独诊断的AUC(0.782、0.764、0.766),差异均有统计学意义(Z=4.315、4.496、4.087,P<0.05)。结论EMs患者血清PDK1、PAX8、DKK1水平均升高,3项指标联合对EMs具有较好的诊断价值。 展开更多
关键词 子宫内膜异位症 3-磷脂酰肌醇依赖性蛋白激酶1 配对盒基因8 dickkopf相关蛋白1 诊断
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